Portable liquid nitrogen freezing device for sample freezing and storage

Abstract

A portable liquid nitrogen freezing device for sample freezing and storage comprises a box body, a sample table, a sampling rod, a sample inlet, a liquid adding opening, a probe and a display screen, the sample inlet, the liquid adding opening and the display screen are all arranged at the top of the box body, the probe is arranged in the box body and fixed to the bottom of the display screen, and a plurality of centrifugal tube grooves are formed in the sample table. A sample inlet cover and a liquid inlet cover which are used for sealing are respectively arranged on the sample inlet and the liquid inlet; and a sampling rod buckle is also arranged on the side surface of the box body. The sample table and the sample inlet with proper sizes can be selected according to the quantity of samples, so that the application range is wide; the sampling rod and the lifting hook ring facilitate entering and exiting of the sample table from the sample inlet, and hands are prevented from being frostbitten during operation; threaded connection is convenient to operate, and waste of liquid nitrogen is reduced; a probe and a display screen are used for monitoring the liquid nitrogen capacity in real time, so that good freezing and storage effects are ensured; a sample table limiting groove is used for conveniently and quickly clamping and limiting the sample table; and the sampling rod is buckled, so that the sampling rod is convenient to store and is prevented from being lost.

Description

Technical Field

[0001] This utility model belongs to the field of sample freezing and storage technology, specifically relating to a portable liquid nitrogen freezing device for sample freezing and storage. Background Technology

[0002] Rapid freezing of leaves with liquid nitrogen is a common technique in molecular laboratories. Utilizing the extremely low temperature (-196℃) of liquid nitrogen, the temperature of leaf tissue is rapidly lowered, fixing the water content within the plant leaves and quickly freezing the sample, which facilitates subsequent leaf grinding and extraction. Liquid nitrogen also plays a crucial role in sample preservation. In the field of biotechnology, positive bacterial strains and viruses, cells, and various biological samples requiring preservation of viability all require cryopreservation. Before cryopreservation, samples need to be pretreated with liquid nitrogen. However, improper or insufficient use of liquid nitrogen is common, not only wasting the nitrogen but also creating unnecessary safety hazards. For example, in the extraction of genomic DNA from tomato leaves, directly pouring liquid nitrogen into a foam box not only causes significant evaporation and waste but also poses a risk of frost damage and may even result in uneven freezing, affecting the quality of DNA extraction. Currently, in research on tomato viral diseases such as tomato brown wrinkle virus and tomato yellow leaf curl virus, effective preservation of positive samples is crucial for virus detection and research. Utility Model Content

[0003] In response to the problems raised in the background technology, this utility model studies and designs a portable liquid nitrogen freezing device for sample freezing and storage. Its purpose is to provide a portable liquid nitrogen freezing device for sample freezing and storage that is safe and convenient to use, has good preservation effect, effectively reduces liquid nitrogen evaporation, and improves liquid nitrogen utilization rate.

[0004] The technical solution of this utility model:

[0005] A portable liquid nitrogen freezing device for sample freezing and storage includes a housing, a sample stage, a sampling rod, a sample inlet, a liquid inlet, a probe, and a display screen. The sample inlet, liquid inlet, and display screen are all located on the top of the housing. The probe is located inside the housing and fixed to the bottom of the display screen. The sample stage is provided with several centrifuge tube slots for placing centrifuge tubes. The sampling rod is used in conjunction with the sample stage. The sample inlet and liquid inlet are respectively provided with a sample inlet cap and a liquid inlet cap for sealing. The side of the housing is also provided with a sampling rod buckle.

[0006] Preferably, an inner liner with the same shape as the box body is tightly attached to the inner side wall of the box, and the inner liner is a silver-plated double-layer vacuum glass structure.

[0007] Preferably, the inlet cap is threaded to the inlet, the liquid filling cap is threaded to the liquid filling port, and the handles of both the inlet cap and the liquid filling cap are provided with anti-slip protrusions.

[0008] Preferably, the sampling rod includes a rod body, a handle, and a hook, with the handle fixedly sleeved at one end of the rod body and the hook disposed at the other end of the rod body.

[0009] Preferably, the sample stage is cylindrical, and a hook ring matching the hook is provided at the middle position of the top of the sample stage.

[0010] Preferably, there are at least two injection ports of different sizes, the diameter of the injection port is slightly larger than the diameter of the sample stage, and the size of the sample stage is adapted to the injection port.

[0011] Preferably, a sample stage limiting groove for engaging the sample stage is provided inside the box and directly below the sample inlet.

[0012] Preferably, on the top of the chamber, the sample inlets are arranged in a row on one side, and the liquid filling port and the display screen are arranged sequentially on the other side.

[0013] Preferably, the probe is electrically connected to the display screen.

[0014] Preferably, the sampling rod buckle is L-shaped, and there are at least two of them, which are fixed to the side of the box for storing the sampling rod.

[0015] The beneficial effects of this utility model are as follows: This utility model has a reasonable structure and novel design, featuring multiple sample inlets of different sizes. The appropriate sample stage and inlet size can be selected based on the quantity of samples, making it widely applicable. The sampling rod and the hook ring on the sample stage facilitate the entry and exit of the sample stage from the inlet, preventing frostbite to hands during sample freezing and storage. When using the model, a suitable sample inlet is selected, and the inlet and inlet cap are threaded together, which not only facilitates operation but also effectively reduces liquid nitrogen evaporation, increases liquid nitrogen utilization, and reduces waste. A probe and display screen monitor the liquid nitrogen volume in the chamber in real time, ensuring good freezing and preservation effects. The sample stage limiting groove, located inside the chamber and directly below the inlet, facilitates quick locking and limiting of the sample stage, preventing samples from tipping over and scattering during chamber movement. The sampling rod buckle facilitates the storage of the sampling rod, preventing loss and demonstrating high practical value. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the box in this utility model;

[0018] Figure 3This is a schematic diagram of the internal structure of the box in this utility model;

[0019] Figure 4 This is a cross-sectional view of the box body in this utility model;

[0020] Figure 5 This is a schematic diagram of the sample stage in this utility model;

[0021] Figure 6 This is a schematic diagram of the sampling rod in use according to this utility model.

[0022] The components are: 1. Box body, 2. Sample stage, 3. Sample inlet, 4. Liquid inlet, 5. Probe, 6. Display screen, 7. Centrifuge tube trough, 8. Sample inlet cover, 9. Liquid inlet cover, 10. Sampling rod buckle, 11. Inner liner, 12. Hand-tightening plate, 13. Anti-slip protrusion, 14. Rod body, 15. Handle, 16. Hook, 17. Hook ring, 18. Sample stage limiting groove. Detailed Implementation

[0023] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0024] like Figures 1-6 As shown, a portable liquid nitrogen freezing device for sample freezing and storage includes a housing 1, a sample stage 2, a sampling rod, a sample inlet 3, a liquid inlet 4, a probe 5, and a display screen 6. The sample inlet 3, the liquid inlet 4, and the display screen 6 are all located on the top of the housing 1. The probe 5 is located inside the housing 1 and fixed to the bottom of the display screen 6. The sample stage 2 is provided with several centrifuge tube slots 7 for placing centrifuge tubes. The sampling rod is used in conjunction with the sample stage 2. The sample inlet 3 and the liquid inlet 4 are respectively provided with a sample inlet cap 8 and a liquid inlet cap 9 for sealing. The side of the housing 1 is also provided with a sampling rod buckle 10.

[0025] like Figure 4 As shown, in this embodiment, an inner liner 11 with the same shape as the box body is attached to the inner wall of the box body 1. The inner liner 11 is a silver-plated double-layer vacuum glass structure.

[0026] like Figures 1-3 As shown, in this embodiment, the inlet cap 8 is connected to the inlet 3 by a thread, and the liquid filling cap 9 is connected to the liquid filling port 4 by a thread. The hand-tightening plate 12 of both the inlet cap 8 and the liquid filling cap 9 is provided with anti-slip protrusions 13 to prevent the hand from slipping when twisting the inlet cap 8 and the liquid filling cap 9.

[0027] like Figure 1 , Figure 6 As shown, in this embodiment, the sampling rod includes a rod body 14, a handle 15, and a hook 16. The handle 15 is fixedly sleeved on one end of the rod body 14, and the hook 16 is disposed on the other end of the rod body 14.

[0028] like Figure 5 As shown, in this embodiment, the sample stage 2 is a cylinder, and a hook ring 17 matching the hook 16 is provided at the middle position of the top of the sample stage 2.

[0029] like Figures 1-3 As shown, in this embodiment, there are at least two injection ports 3 of different sizes. The diameter of the injection port 3 is slightly larger than the diameter of the sample stage 2. The appropriate size of the sample stage 2 is selected according to the amount of sample, so that the injection port 3 is selected in a suitable position. That is, the size of the sample stage 2 is adapted to the injection port 3.

[0030] like Figure 3 As shown, in this embodiment, a sample stage limiting groove 18 for engaging the sample stage 2 is provided inside the housing 1 and directly below the sample inlet 3.

[0031] like Figures 1-3 As shown in this embodiment, on the top of the box 1, the sample inlet 3 is arranged in a row on one side, and the liquid filling port 4 and the display screen 6 are arranged in sequence on the other side.

[0032] like Figure 3 As shown, in this embodiment, probe 5 is electrically connected to display screen 6. The structure of display screen 6 and how it works are existing technologies and will not be described in detail here.

[0033] like Figures 1-3 As shown, in this embodiment, the sampling rod buckle 10 is L-shaped, and there are at least two of them. They are fixed to the side of the box 1 for storing the sampling rod.

[0034] Specific usage procedure: In actual use, after gripping the handle 12 with your thumb and forefinger, unscrew the liquid inlet cap 9. Add liquid nitrogen into the chamber 1 through the liquid inlet 4, and monitor the liquid nitrogen volume in the chamber 1 with the probe 5. When the liquid nitrogen volume in the chamber 1 is sufficient, continue to grip the handle 12 with your thumb and forefinger and screw the liquid inlet cap 9 onto the liquid inlet 4. Select a suitable sample stage 2 according to the number of centrifuge tubes. After placing the centrifuge tubes in the centrifuge tube slot of the sample stage 2, hold the sampling rod handle 15 and hook the hook ring 17 on the sample stage 2 with the hook 16. After gripping the hand-tightening plate 12, unscrew the inlet cap 8 on the corresponding inlet 3, and place the sample stage 2 into the sample stage limiting groove 18 inside the box 1 through the inlet 3. Take out the sampling rod, and continue to grip the hand-tightening plate 12 with your thumb and forefinger. The inlet cap 8 will then be on the inlet 3, sealing the box 1. The sample in the centrifuge tube will be frozen and stored inside the box 1. The rod body 14 of the sampling rod will be engaged in the L-shaped sampling rod buckle 10. The probe 5 will monitor the liquid nitrogen capacity inside the box 1 and transmit the signal to the display screen 6, thereby replenishing liquid nitrogen into the box 1 in a timely manner. This invention features multiple sample inlets 3 of varying sizes, allowing for selection of the appropriate sample stage 2 and inlet 3 based on the quantity of samples, thus broadening its applicability. The sampling rod and hook ring 17 on the sample stage 2 facilitate easy access to and from the inlet 3, preventing frostbite to hands during sample freezing and storage. Selecting the appropriate inlet 3 and threaded connection between the inlet 3 and the inlet cap 8 not only facilitates operation but also effectively reduces liquid nitrogen evaporation, increases liquid nitrogen utilization, and minimizes waste. A probe 5 and display screen 6 monitor the liquid nitrogen capacity within the chamber 1 in real time, ensuring optimal freezing and preservation. A sample stage limiting groove 18, located inside the chamber 1 and directly below the inlet 3, facilitates quick locking and limiting of the sample stage 2, preventing sample spillage during chamber 1 movement. A sampling rod buckle 10 facilitates convenient storage of the sampling rod, preventing loss and demonstrating high practical value.

[0035] In summary, this utility model has achieved the expected results.

[0036] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present utility model. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.

Claims

1. A portable liquid nitrogen freezing device for sample freezing and storage, characterized in that: The device includes a housing, a sample stage, a sampling rod, an inlet, a liquid inlet, a probe, and a display screen. The inlet, liquid inlet, and display screen are all located on the top of the housing. The probe is located inside the housing and fixed to the bottom of the display screen. The sample stage has several centrifuge tube slots for placing centrifuge tubes. The sampling rod is used in conjunction with the sample stage. The inlet and liquid inlet are respectively equipped with inlet caps and liquid inlet caps for sealing. The side of the housing also has a sampling rod buckle. The sampling rod includes a rod body, a handle, and a hook. The handle is fixedly sleeved on one end of the rod body, and the hook is located on the other end of the rod body. There are at least two inlets of different sizes, with the diameter of the inlet slightly larger than the diameter of the sample stage. The size of the sample stage is adapted to the size of the inlets.

2. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The inner wall of the box is tightly fitted with an inner liner that is the same shape as the box body. The inner liner is a silver-plated double-layer vacuum glass structure.

3. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The inlet cap is threaded to the inlet, and the liquid filling cap is threaded to the liquid filling port. Both the inlet cap and the liquid filling cap have anti-slip protrusions on their handles.

4. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The sample stage is cylindrical, and a hook ring matching the hook is provided at the middle of the top of the sample stage.

5. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The sample stage limiting groove is provided inside the box and directly below the sample inlet for locking the sample stage.

6. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The top of the chamber has a row of sample inlets on one side, and a liquid filling port and a display screen on the other side.

7. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The probe is electrically connected to the display screen.

8. The portable liquid nitrogen freezing device for sample freezing and storage as described in claim 1, characterized in that: The sampling rod clips are L-shaped, and there are at least two of them. They are fixed to the side of the box and used for storing the sampling rods.

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