Multifunctional fermentation tank for NAD < + > biological preparation

Abstract

The utility model discloses a multifunctional fermentation tank for NAD < + > biological preparation, and relates to the technical field of biological preparation, the multifunctional fermentation tank comprises a fermentation tank main body, a limiting ring and a gear ring, the upper side of the fermentation tank main body is provided with a stirring mechanism; a rotating ring is rotationally connected to the inner side of the limiting ring, a mounting frame is fixedly connected to the bottom of the rotating ring, a constant-temperature mechanism is fixedly mounted on one side of the mounting frame, a scraper is fixedly connected to the other side of the mounting frame, and a rotating mechanism is mounted on the upper side of the fermentation tank main body. The device realizes mild and efficient fermentation and mixing through flexible stirring design, avoids microbial cell damage, improves product integrity, eliminates local temperature difference by combining a dynamic constant temperature technology and utilizing heat uniform diffusion and stirring synergistic effect, remarkably improves NAD + synthesis efficiency and batch consistency, and meanwhile, through a scraper follow-up cleaning function, the device has the advantages of simple structure and low cost. The stirring effect is enhanced, fermentation residues are thoroughly removed, the cleaning process is simplified, and the sanitary safety of rapid reuse and continuous production of equipment is ensured.

Description

Technical Field

[0001] This utility model relates to the field of biopreparation technology, and more specifically, to a multifunctional fermenter for NAD+ biopreparation. Background Technology

[0002] NAD+ (nicotinamide adenine dinucleotide), as an important coenzyme in organisms, is widely involved in key biological processes such as energy metabolism, redox reactions, and cell signaling. In recent years, with the rapid development of biomedicine, functional foods, and cosmetics, the demand for NAD+ has increased significantly, making its efficient and stable biosynthesis technology a research hotspot. Fermentation, due to its advantages of being environmentally friendly, low-cost, and easy to scale up, has become one of the main routes for NAD+ preparation. However, traditional fermenters face many technical bottlenecks in the NAD+ biosynthesis process, restricting further improvements in production efficiency and product quality.

[0003] First, traditional fermenters often use rigid stirring blades for their stirring mechanisms. While these blades can mix the fermentation broth, they can also cause mechanical damage to microbial cells or sensitive biological components during high-speed stirring, leading to reduced fermentation activity or even product loss. Furthermore, rigid stirring structures can generate localized shear forces, disrupting the metabolic balance of microorganisms and affecting the synthesis efficiency of NAD+. Second, traditional fermenters often use static heating or cooling systems, which makes it difficult to achieve uniform temperature distribution throughout the fermentation system. This can easily lead to localized overheating or overcooling, causing abnormal microbial metabolism and affecting the yield and batch consistency of NAD+. Therefore, to address these technical problems, a multifunctional fermenter for NAD+ bioprocessing is proposed. Utility Model Content

[0004] The purpose of this invention is to provide a multifunctional fermenter for NAD+ bioprocessing. Through a flexible stirring design, it achieves gentle and efficient fermentation mixing, avoids damage to microbial cells, and improves product integrity. Furthermore, by combining dynamic constant temperature technology, it utilizes the synergistic effect of uniform heat diffusion and stirring to eliminate local temperature differences, significantly improving the efficiency and batch consistency of NAD+ synthesis. At the same time, the scraper-driven cleaning function enhances the stirring effect and thoroughly removes fermentation residues, simplifies the cleaning process, and ensures the hygiene and safety of rapid equipment reuse and continuous production.

[0005] This utility model is achieved through the following technical solution:

[0006] A multifunctional fermenter for NAD+ bioprocessing, comprising:

[0007] The main body of the fermentation tank is equipped with a stirring mechanism on its upper side;

[0008] A limiting ring is fixedly connected to the inner side of the fermenter body. A rotating ring is rotatably connected to the inner side of the limiting ring. A mounting bracket is fixedly connected to the bottom of the rotating ring. A constant temperature mechanism is fixedly installed on one side of the mounting bracket, and a scraper is fixedly connected to the other side of the mounting bracket. The scraper is in contact with the inner wall of the fermenter body.

[0009] A toothed ring is fixedly connected to the inner side of the rotating ring, and a rotating mechanism is installed on the upper side of the fermenter body.

[0010] Preferably, a feeding pipe is fixedly connected to the upper side of the fermentation tank body, and a cap is threadedly connected to the upper side of the feeding pipe.

[0011] Preferably, the stirring mechanism includes a first motor, a rotating rod, a stirring rod, and a flexible sleeve. The first motor is fixedly connected to the upper side of the cover, the rotating rod is fixedly connected to the lower side of the first motor, and the rotating rod is rotatably connected to the inner side of the fermentation tank body. The stirring rod is fixedly connected to the outside of the rotating rod, and there are several groups of stirring rods arranged symmetrically. The flexible sleeve is fixedly connected to the outside of the stirring rod. The flexible sleeve is made of plastic material, and the outer surface of the flexible sleeve is smooth and without sharp edges.

[0012] Preferably, the constant temperature mechanism includes a fixed base, a mounting rod, and a thermoelectric component. The fixed base and the mounting frame are fixedly connected. The mounting rod is fixedly connected to the inside of the fixed base, and the thermoelectric component is fixedly connected to the outside of the mounting rod.

[0013] Preferably, a thermoplastic polyurethane film is provided on the outside of the fixing base, and the thermoplastic polyurethane film has waterproof and thermal conductivity. A partition net is fixedly connected to the inside of the fixing base, and the partition net is located between the thermoplastic polyurethane film and the thermoelectric component.

[0014] Preferably, the rotating mechanism includes a second motor, a rotating shaft, and a gear. The second motor is fixedly connected to the upper side of the fermenter body, the rotating shaft is fixedly connected to the lower side of the second motor and rotatably connected to the inner side of the fermenter body, and the gear is fixedly connected to the outside of the rotating shaft, and the gear meshes with the gear ring.

[0015] Preferably, an output pump is fixedly connected to the bottom inner side of the fermenter body, and an output pipe is fixedly connected to the lower side of the output pump, with the end of the output pipe passing through the bottom of the fermenter body and extending out of the fermenter body.

[0016] Preferably, the main body of the fermenter is externally fixedly connected with support legs, and the number of support legs is several sets arranged symmetrically.

[0017] The technical solution of this utility model has at least the following beneficial effects:

[0018] 1. This multifunctional fermenter for NAD+ bioprocessing features a flexible stirring design with a smooth, edgeless plastic material covering the stirring rod. This design achieves efficient mixing while avoiding mechanical damage to microbial cells or sensitive components, significantly improving fermentation activity and product integrity. It is especially suitable for NAD+ bioprocessing under high-speed stirring conditions.

[0019] 2. This multifunctional fermenter for NAD+ bioprocessing combines dynamic temperature control with scraper cleaning. It utilizes the synergistic effect of thermoelectric components and rotating rings to achieve precise temperature control across the entire range, eliminating localized temperature deviations. At the same time, the scraper's follow-up movement enhances the stirring effect and thoroughly removes residues inside the tank. This not only improves fermentation efficiency and batch consistency but also significantly simplifies the cleaning process, ensuring rapid equipment reuse and meeting the high efficiency and hygiene requirements of continuous production. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 for Figure 1 Enlarged view of A in the middle;

[0022] Figure 3 This is a partial structural schematic diagram of the present invention;

[0023] Figure 4 for Figure 3 Enlarged view of B

[0024] Figure 5 This is a partial structural front sectional view of the present invention;

[0025] Icons: 1. Fermentation tank body; 2. Feeding pipe; 3. Cover; 4. First motor; 5. Rotating rod; 6. Stirring rod; 7. Flexible sleeve; 8. Limiting ring; 9. Rotating ring; 10. Mounting frame; 11. Fixing base; 12. Mounting rod; 13. Thermoelectric assembly; 14. Thermoplastic polyurethane film; 15. Partition net; 16. Gear ring; 17. Second motor; 18. Rotating shaft; 19. Gear; 20. Scraper; 21. Output pump; 22. Output pipe; 23. Support foot. Detailed Implementation

[0026] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0027] Example:

[0028] Please see Figures 1-5 This application proposes a multifunctional fermenter for NAD+ bioprocessing, comprising a fermenter body 1, a limiting ring 8, and a toothed ring 16. The fermenter body 1 has a stirring mechanism installed on its upper side. The limiting ring 8 is fixedly connected to the inner side of the fermenter body 1, and a rotating ring 9 is rotatably connected to the inner side of the limiting ring 8. A mounting frame 10 is fixedly connected to the bottom of the rotating ring 9. A temperature control mechanism is fixedly installed on one side of the mounting frame 10, and a scraper 20 is fixedly connected to the other side of the mounting frame 10, with the scraper 20 fitting against the inner wall of the fermenter body 1. The toothed ring 16 is fixedly connected to the inner side of the rotating ring 9, and a rotating mechanism is installed on the upper side of the fermenter body 1.

[0029] The upper side of the fermentation tank body 1 is fixedly connected to the feeding pipe 2, and the upper side of the feeding pipe 2 is threadedly connected to the cap 3. The design of the feeding pipe 2 facilitates the rapid addition of materials, and the threaded connection of the cap 3 ensures airtightness and prevents external contamination from entering the tank.

[0030] The stirring mechanism includes a first motor 4, a rotating rod 5, a stirring rod 6, and a flexible sleeve 7. The first motor 4 is fixedly connected to the upper side of the cover 3, the rotating rod 5 is fixedly connected to the lower side of the first motor 4, and the rotating rod 5 is rotatably connected to the inner side of the fermentation tank body 1. The stirring rod 6 is fixedly connected to the outside of the rotating rod 5, and there are several groups of stirring rods 6 arranged symmetrically. The flexible sleeve 7 is fixedly connected to the outside of the stirring rod 6. The flexible sleeve 7 is made of plastic material, and the outer surface of the flexible sleeve 7 is smooth and without sharp edges. The design of the flexible sleeve 7 not only reduces the stirring resistance, but also effectively protects the microbial cells in the fermentation material and avoids damage caused by high-speed stirring.

[0031] The temperature control mechanism includes a fixed base 11, a mounting rod 12, and a thermoelectric component 13. The fixed base 11 is fixedly connected to the mounting frame 10. The mounting rod 12 is fixedly connected to the inside of the fixed base 11, and the thermoelectric component 13 is fixedly connected to the outside of the mounting rod 12. The thermoelectric component 13 is stably connected to the fixed base 11 through the mounting rod 12, ensuring that heat is efficiently transferred to the fermentation liquid and achieving precise temperature control.

[0032] A thermoplastic polyurethane film 14 is provided on the outside of the fixing base 11. The thermoplastic polyurethane film 14 is waterproof and thermally conductive. A mesh 15 is fixedly connected to the inside of the fixing base 11. The mesh 15 is located between the thermoplastic polyurethane film 14 and the thermoelectric component 13. The high thermal conductivity and waterproofness of the thermoplastic polyurethane film 14 further improve the uniformity of heat distribution.

[0033] The rotating mechanism includes a second motor 17, a rotating shaft 18, and a gear 19. The second motor 17 is fixedly connected to the upper side of the fermenter body 1, the rotating shaft 18 is fixedly connected to the lower side of the second motor 17, and the rotating shaft 18 is rotatably connected to the inner side of the fermenter body 1. The gear 19 is fixedly connected to the outside of the rotating shaft 18, and the gear 19 meshes with the gear ring 16. The meshing design of the gear 19 and the gear ring 16 ensures the smooth operation of the rotating ring 9 and realizes the dynamic coverage of the constant temperature mechanism.

[0034] An output pump 21 is fixedly connected to the bottom inner side of the fermentation tank body 1. An output pipe 22 is fixedly connected to the lower side of the output pump 21. The end of the output pipe 22 passes through the bottom of the fermentation tank body 1 and extends out of the fermentation tank body 1. The combined design of the output pump 21 and the output pipe 22 facilitates the efficient discharge of fermentation products and reduces residues.

[0035] The fermenter body 1 is externally fixedly connected with support legs 23, and there are several sets of support legs 23 arranged symmetrically. The symmetrical distribution of support legs 23 ensures the stability of the fermenter and is suitable for different working environments.

[0036] The working principle of the multifunctional fermenter for NAD+ bioprocessing based on the embodiment is as follows: Upon fermentation start-up, the first motor 4 drives the rotating rod 5 to rotate multiple symmetrically distributed stirring rods 6, thoroughly mixing the fermentation material inside the tank and promoting microbial metabolism and reaction homogenization. The flexible sleeve 7 wrapped around the stirring rods 6 is made of smooth, edgeless plastic material, which reduces stirring resistance, improves fluidity, and avoids mechanical damage to microbial cells or sensitive components caused by traditional rigid structures through flexible contact. Especially during high-speed stirring, it effectively prevents the breakage of biological components, ensuring fermentation activity and product integrity. During stirring, the thermoelectric component 13 is energized and generates heat, which is transferred to the thermoplastic polyurethane film 14, utilizing its high thermal conductivity. The temperature is evenly diffused into the fermentation broth. Simultaneously, the second motor 17 drives the gear 19 at the end of the rotating shaft 18 to mesh with the gear ring 16, causing the rotating ring 9 and the mounting frame 10 to rotate circumferentially along the limiting ring 8. This allows the constant temperature mechanism to dynamically cover the inner wall area of ​​the fermenter, eliminating localized temperature deviations. This design, combining dynamic heat distribution with the synergistic effect of stirring, achieves precise temperature control throughout the entire process, significantly improving the efficiency and batch consistency of NAD+ synthesis. As the rotating ring 9 rotates, the scraper 20 on the side of the mounting frame 10 moves in conjunction with the inner wall of the tank. This enhances the turbulence effect of the fermentation broth, assisting the stirring mechanism in breaking the concentration gradient. Furthermore, it can scrape away fermentation residues adhering to the wall surface in real time, preventing the risk of contamination caused by biofilm accumulation. After fermentation, the output pump 21 discharges the product through the output pipe 22. The continuous operation of the scraper 20 thoroughly removes residual materials from the bottom and walls of the tank, greatly reducing the difficulty of manual cleaning, ensuring rapid equipment reuse, and meeting the hygiene and efficiency requirements of continuous production.

[0037] Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multifunctional fermenter for NAD+ bioprocessing, characterized in that, include: The main body of the fermentation tank (1) is equipped with a stirring mechanism on its upper side; A limiting ring (8) is fixedly connected to the inner side of the fermenter body (1). A rotating ring (9) is rotatably connected to the inner side of the limiting ring (8). A mounting bracket (10) is fixedly connected to the bottom of the rotating ring (9). A constant temperature mechanism is fixedly installed on one side of the mounting bracket (10), and a scraper (20) is fixedly connected to the other side of the mounting bracket (10). The scraper (20) is in contact with the inner wall of the fermenter body (1). A toothed ring (16) is fixedly connected to the inner side of the rotating ring (9), and a rotating mechanism is installed on the upper side of the fermenter body (1).

2. The multifunctional fermenter for NAD+ bioprocessing according to claim 1, characterized in that: The upper side of the fermenter body (1) is fixedly connected to a feeding pipe (2), and the upper side of the feeding pipe (2) is threadedly connected to a cap (3).

3. The multifunctional fermenter for NAD+ bioprocessing according to claim 1, characterized in that: The stirring mechanism includes a first motor (4), a rotating rod (5), a stirring rod (6), and a flexible sleeve (7). The first motor (4) is fixedly connected to the upper side of the cover (3). The rotating rod (5) is fixedly connected to the lower side of the first motor (4) and is rotatably connected to the inner side of the fermentation tank body (1). The stirring rod (6) is fixedly connected to the outside of the rotating rod (5). The number of stirring rods (6) is several groups and they are arranged symmetrically. The flexible sleeve (7) is fixedly connected to the outside of the stirring rod (6). The flexible sleeve (7) is made of plastic material and has a smooth outer surface without sharp edges.

4. The multifunctional fermenter for NAD+ bioprocessing according to claim 1, characterized in that: The constant temperature mechanism includes a fixed base (11), a mounting rod (12), and a thermoelectric component (13). The fixed base (11) is fixedly connected to the mounting bracket (10). The mounting rod (12) is fixedly connected to the inside of the fixed base (11), and the thermoelectric component (13) is fixedly connected to the outside of the mounting rod (12).

5. The multifunctional fermenter for NAD+ bioprocessing according to claim 4, characterized in that: The fixing base (11) has a thermoplastic polyurethane film (14) on its outside, and the thermoplastic polyurethane film (14) is waterproof and thermally conductive. A mesh (15) is fixedly connected to the inside of the fixing base (11), and the mesh (15) is located between the thermoplastic polyurethane film (14) and the thermoelectric component (13).

6. The multifunctional fermenter for NAD+ bioprocessing according to claim 1, characterized in that: The rotating mechanism includes a second motor (17), a rotating shaft (18), and a gear (19). The second motor (17) is fixedly connected to the upper side of the fermenter body (1). The rotating shaft (18) is fixedly connected to the lower side of the second motor (17) and is rotatably connected to the inner side of the fermenter body (1). The gear (19) is fixedly connected to the outside of the rotating shaft (18) and meshes with the gear ring (16).

7. The multifunctional fermenter for NAD+ bioprocessing according to claim 1, characterized in that: An output pump (21) is fixedly connected to the bottom inner side of the fermentation tank body (1), and an output pipe (22) is fixedly connected to the lower side of the output pump (21). The end of the output pipe (22) passes through the bottom of the fermentation tank body (1) and extends out of the fermentation tank body (1).

8. The multifunctional fermenter for NAD+ bioprocessing according to claim 1, characterized in that: The fermenter body (1) is fixedly connected to the outside of the support legs (23), and the number of support legs (23) is several groups arranged symmetrically.

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