Sample pretreatment tube

Abstract

The utility model discloses a sample pretreatment tube which comprises a sample treatment tube and a sample collection tube, a liquid adding opening and a sealing piece for sealing the liquid adding opening are arranged at the top of the sample treatment tube; a liquid outlet is formed in the bottom of the sample treatment tube; the sample collection pipe is positioned below the sample treatment pipe and is used for collecting the pretreatment sample liquid flowing out of the liquid outlet; and an adsorption material for adsorbing red blood cells is arranged in the sample treatment tube. When the sample pretreatment tube disclosed by the utility model is used, the sealing element is opened, a sample diluent and a whole blood sample are sequentially added into the sample pretreatment tube, and the sample diluent and the whole blood sample can be uniformly mixed in the sample pretreatment tube to obtain a sample solution due to the flow choking effect of the adsorption material on the sample diluent and the whole blood sample; then the sample liquid passes through the adsorption material under the action of seepage, the adsorption material adsorbs liquid such as red blood cells and plasma in the sample liquid, the liquid passes through the adsorption material and then enters the sample collection tube, sample pretreatment is completed, and a pretreated sample liquid is obtained.

Description

Technical Field

[0001] This invention belongs to the field of in vitro diagnostic technology, specifically a sample pretreatment tube. Background Technology

[0002] Chromatographic test strips utilize antibody-antigen reactions through a chromatographic process to quickly and easily detect specific components in a sample. The basic structure of a traditional chromatographic test strip includes a sample pad, a conjugate pad, an NC membrane, and absorbent paper. The sample pad, located on the top layer of the test strip, primarily absorbs the added sample solution, helping to evenly distribute the sample and transfer it to the next layer. Therefore, the sample pad has certain sample pretreatment functions: First, it treats anti-erythrocyte antibodies, filtering out red blood cells and plasma from whole blood samples for subsequent reaction steps; second, it contains blocking agents to eliminate interference from heterophilic antibodies or RF in blood samples; third, components in the sample pad can treat the sample, such as adjusting pH, promoting the release of target substances, or stabilizing them.

[0003] However, traditional test strips using a sample pad structure for sample pretreatment also have significant drawbacks. Firstly, they are sensitive to temperature and humidity. The red blood cell antibodies and blocking agents treated on the sample pad are bioactive materials, greatly affected by environmental conditions (especially temperature and humidity). Under extreme conditions, their activity decreases or even becomes inactive, impacting the efficiency and stability of sample treatment. Secondly, after the sample is added, it rapidly passes through the sample pad to the next stage of the structure, making it difficult to ensure thorough mixing of the effective components treated on the pad, thus significantly reducing the effectiveness of the pretreatment. Utility Model Content

[0004] In view of this, the purpose of this utility model is to provide a sample pretreatment tube that can effectively improve sample pretreatment capabilities.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A sample pretreatment tube includes a sample processing tube and a sample collection tube;

[0007] The sample processing tube has a liquid inlet at the top and a seal for sealing the liquid inlet; the sample processing tube has an outlet at the bottom; the sample collection tube is located below the sample processing tube and is used to collect the pre-processed sample liquid flowing out from the outlet; the sample processing tube is equipped with an adsorbent material for adsorbing red blood cells.

[0008] Furthermore, the sample processing tube contains a drying treatment substance, which is located above the adsorbent material.

[0009] Furthermore, the drying treatment material is disposed on the inner wall of the sample processing tube.

[0010] Furthermore, the drying treatment substance includes at least one of buffer solution, salt ions, protective agent, anticoagulant, and blocking agent.

[0011] Furthermore, the adsorption material is a blood filtration membrane or ultrafine glass fiber.

[0012] Furthermore, the sealing element is a sealing cap covering the liquid inlet; or, the sealing element is a sealing piston that fits tightly with the sample processing tube.

[0013] Furthermore, the bottom of the sample collection tube is provided with a puncture port for puncturing and aspirating the sample with a sampling needle, and a puncture membrane is provided inside the puncture port; or, the bottom of the sample collection tube is provided with a bottom outlet, and a sealing membrane is provided over the bottom outlet.

[0014] Furthermore, the sample collection tube is equipped with a marker.

[0015] Furthermore, the upper part of the sample collection tube is sleeved outside the sample processing tube, and the sample collection tube and the sample processing tube are sealed together.

[0016] Furthermore, the sample collection tube and the sample processing tube are axially constrained; or, the sample processing tube can move relative to the sample collection tube in the axial direction.

[0017] Furthermore, the sample collection tube and the sample processing tube are integrated into one unit.

[0018] The beneficial effects of this utility model are as follows:

[0019] In this sample pretreatment tube, the seal is opened, and sample diluent and whole blood sample are added sequentially. Due to the flow-blocking effect of the adsorbent material on the sample diluent and whole blood sample, they remain in the sample pretreatment tube. At this time, the sample diluent and whole blood sample can be mixed in the sample pretreatment tube to obtain sample solution. Then, the sample solution passes through the adsorbent material through permeation. The adsorbent material adsorbs red blood cells, plasma, and other liquids in the sample solution. After passing through the adsorbent material, the solution flows out from the outlet and enters the sample collection tube, completing the sample pretreatment and obtaining the pretreated sample solution. Then, the pretreated sample solution collected in the sample collection tube can be added to a chromatography reagent card for chromatography reaction. In summary, this sample pretreatment tube can effectively improve the sample pretreatment capacity, and the sample pretreatment tube can be manufactured in various specifications to meet the sample processing needs of different volumes. It can be used for both high and low sample throughput, and the production cost is controllable. Attached Figure Description

[0020] To make the objectives, technical solutions, and beneficial effects of this utility model clearer, the following drawings are provided for illustration:

[0021] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the sample pretreatment tube of this utility model;

[0022] Figure 2 This is a schematic diagram of the structure of Embodiment 2 of the sample pretreatment tube of this utility model;

[0023] Figure 3 This is a schematic diagram of the structure of Embodiment 3 of the sample pretreatment tube of this utility model;

[0024] Figure 4 This is a schematic diagram of the structure of Embodiment 4 of the sample pretreatment tube of this utility model;

[0025] Figure 5 This is a schematic diagram of the structure of embodiment 5 of the sample pretreatment tube of this utility model;

[0026] Figure 6 This is a schematic diagram of the structure of Embodiment 6 of the sample pretreatment tube of this utility model;

[0027] Figure 7 This is a schematic diagram of the structure of embodiment 7 of the sample pretreatment tube of this utility model;

[0028] Figure 8 This is a schematic diagram of the structure of Embodiment 8 of the sample pretreatment tube of this utility model.

[0029] Explanation of reference numerals in the attached figures:

[0030] 10-Sample processing tube; 11-Liquid filling port; 12-Seal; 13-Liquid outlet; 14-Adsorbent material; 15-Drying treatment substance; 16-Lower piston;

[0031] 20-Sample collection tube; 21-Marker; 22-Sealing ring; 23-Puncture port; 24-Puncture membrane; 25-Bottom outlet; 26-Sealing membrane. Detailed Implementation

[0032] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, so that those skilled in the art can better understand and implement the present invention. However, the embodiments are not intended to limit the present invention.

[0033] Example 1

[0034] like Figure 1As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0035] In this embodiment, the sample processing tube 10 is equipped with an adsorbent material 14 for adsorbing red blood cells. Specifically, the adsorbent material 14 is a blood filtration membrane or ultrafine glass fiber, which can achieve the technical purpose of adsorbing red blood cells in the sample solution by physical means. Using an adsorbent material to adsorb red blood cells by physical adsorption has the advantages of stable filtration effect and fast filtration speed, while being unaffected by changes in the activity of anti-red blood cell antibodies and avoiding the influence of non-specific cross-reactions of antibodies.

[0036] In this embodiment, a drying agent 15 is provided inside the sample processing tube 10, and the drying agent 15 is located above the adsorbent material 14. In a preferred embodiment, the drying agent 15 is disposed on the inner wall of the sample processing tube 10, that is, the drying agent 15 is a coating layer disposed on the inner wall of the sample processing tube 10. When diluent and whole blood sample are added to the sample processing tube 10, during the mixing process of the diluent and whole blood sample, the diluent and whole blood sample can also be fully mixed with the drying agent 15. Specifically, the drying agent 15 includes at least one of buffer, salt ions, protective agent, anticoagulant, and blocking agent. When the drying agent 15 includes a blocking agent, it can effectively eliminate heterophilic antibodies or RF interference in the sample.

[0037] In this embodiment, the sealing element 12 is a sealing cap that covers the liquid inlet 11, and the sealing cap keeps the liquid inlet 11 sealed. When it is necessary to add diluent and whole blood sample into the sample processing tube 10, the sealing cap is opened and the diluent and whole blood sample are added; after the diluent and whole blood sample are added, the sealing cap is placed on the liquid inlet 11 to maintain the sealed state inside the sample processing tube 10.

[0038] In a preferred embodiment of this example, a marker 21 is provided in the sample collection tube 20. After the pre-processed sample solution enters the sample collection tube 20, the marker 21 dissolves and mixes rapidly with the pre-processed sample solution to carry out a marker incubation reaction.

[0039] In this embodiment, the upper part of the sample collection tube 20 is fitted over the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are in a sealed fit. Specifically, at least one sealing ring 22 is provided between the sample collection tube 20 and the sample processing tube 10 to ensure the sealing performance of the internal space of the sample processing tube 10 and the sample collection tube 20, facilitating the sealed drying and preservation of the dried treatment material 15 and the marker 21. It can be opened before use, which is beneficial for long-term preservation. In this embodiment, the sample collection tube 20 and the sample processing tube 10 are axially limited. Specifically, an axial limiting structure is provided between the sample collection tube 20 and the sample processing tube 10. The axial limiting structure can be implemented in various existing ways, such as by providing an axial limiting ring on the outer peripheral wall of the sample processing tube 10. In this embodiment, the sample processing tube 10 includes a first large-diameter section at the top and a first small-diameter section at the bottom. The outer diameter of the first large-diameter section is larger than the outer diameter of the first small-diameter section, and the outer diameter of the first large-diameter section is larger than the inner diameter of the sample collection tube 20. The outer diameter of the first small-diameter section is smaller than the inner diameter of the sample collection tube 20. In this way, the first small-diameter section of the sample processing tube 10 can be fitted inside the sample collection tube 20, and the axis is limited by the first large-diameter section of the sample processing tube 10.

[0040] Specifically, in this embodiment, after obtaining the pre-treated sample solution, the sample processing tube 10 can be removed from the sample collection tube 20. At this time, the opening of the sample collection tube 20 is open, and the pre-treated sample can be aspirated by inserting a sampling needle into the sample collection tube 20. Then, the pre-treated sample solution is transferred to the chromatography reagent card for chromatography reaction using the sampling needle.

[0041] Specifically, in this embodiment, the sample pretreatment tube is used by opening the sealed cap and sequentially adding sample diluent and whole blood sample into the sample treatment tube 10. Due to the flow-blocking effect of the adsorbent material 14 on the sample diluent and whole blood sample, the sample diluent and whole blood sample will remain in the sample treatment tube 10. At this time, the sample diluent and whole blood sample can be mixed in the sample treatment tube 10 to obtain a sample solution. Then, the sample solution passes through the adsorbent material 14 through percolation. The adsorbent material 14 adsorbs red blood cells, plasma, and other liquids in the sample solution and enters the sample collection tube 20 after passing through the adsorbent material 14, completing the sample pretreatment and obtaining the pretreated sample solution. Then, the pretreated sample solution collected in the sample collection tube 20 can be added to a chromatography reagent card for chromatography reaction. In summary, the sample pretreatment tube of this embodiment can effectively improve the sample pretreatment capacity, and the sample pretreatment tube can be manufactured in various specifications to meet the sample processing needs of different volumes. It can be used for both high and low sample throughput, and the production cost is controllable. Specifically, when the pretreated sample solution obtained from the sample pretreatment tube of this embodiment is added to the chromatography reagent card, it is significantly superior to traditional test strips in many aspects, such as reaction kinetic curve control, precision, whole blood filtration, and anti-interference ability.

[0042] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0043] The sample preprocessing method of this embodiment includes the following steps:

[0044] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0045] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0046] In a preferred embodiment of this example, the sample processing tube 10 is provided with a drying treatment substance 15, which is mixed with the diluent and whole blood sample when the diluent and whole blood sample are mixed.

[0047] The sample chromatography method of this embodiment includes the following steps:

[0048] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0049] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0050] Specifically, in this embodiment, after obtaining the pre-treated sample solution, the sample processing tube 10 can be removed from the sample collection tube 20. At this time, the opening of the sample collection tube 20 is open, and the pre-treated sample can be aspirated by inserting a sampling needle into the sample collection tube 20. Then, the pre-treated sample solution is transferred to the chromatography reagent card for chromatography reaction using the sampling needle.

[0051] In a preferred embodiment of this example, a marker 21 is provided in the sample collection tube 20. After the pre-processed sample solution enters the sample collection tube 20, the marker 21 dissolves and mixes rapidly with the pre-processed sample solution to carry out a marker incubation reaction.

[0052] Example 2

[0053] like Figure 2 As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0054] In this embodiment, the upper part of the sample collection tube 20 is fitted over the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are sealed together. An axial limiting structure is provided between the sample collection tube 20 and the sample processing tube 10. In this embodiment, the bottom of the sample collection tube 20 is provided with a puncture port 23 for aspiration needle puncture, and a puncture membrane 24 is provided inside the puncture port 23. After the aspiration needle punctures the puncture membrane 24, it enters the sample collection tube 20 through the puncture port 23 to aspirate the pre-treated sample solution, facilitating the transfer of the pre-treated sample solution to the chromatography reagent card for chromatography reaction.

[0055] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0056] The sample preprocessing method of this embodiment includes the following steps:

[0057] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0058] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0059] The sample chromatography method of this embodiment includes the following steps:

[0060] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0061] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0062] Specifically, in this embodiment, after obtaining the pretreated sample solution, the sampling needle pierces the puncture membrane 24 and enters the sample collection tube 20 through the puncture port 23 to draw the pretreated sample solution. Then, the sampling needle is used to transfer the pretreated sample solution to the chromatography reagent card for chromatography reaction.

[0063] The other specific implementation methods in this embodiment are the same as those in Embodiment 1, and will not be described in detail hereafter.

[0064] Example 3

[0065] like Figure 3 As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0066] In this embodiment, the upper part of the sample collection tube 20 is fitted over the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are in a sealed fit. In this embodiment, the sample processing tube 10 can move axially relative to the sample collection tube 20. Specifically, a lower piston 16 is fitted over the lower end of the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are in a sealed fit through the lower piston 16. The bottom of the sample collection tube 20 is provided with a bottom outlet 25, which is covered with a sealing film 26. Thus, after the pretreatment sample solution is collected in the sample collection tube 20, the sealing film 26 can be torn open, driving the sample processing tube 10 to move axially downward relative to the sample collection tube 20. Under the action of the lower piston 16, the pretreatment sample solution in the sample collection tube 20 is driven to flow out from the bottom outlet 25 and transferred to the chromatography reagent card for chromatography reaction.

[0067] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0068] The sample preprocessing method of this embodiment includes the following steps:

[0069] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0070] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0071] The sample chromatography method of this embodiment includes the following steps:

[0072] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0073] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0074] Specifically, in this embodiment, after obtaining the pre-treated sample solution, the sealing film 26 is torn open, and the sample processing tube 10 is driven to move axially downward relative to the sample collection tube 20. Under the action of the lower piston 16, the pre-treated sample solution in the sample collection tube 20 is driven to flow out from the bottom outlet 25 and transferred to the chromatography reagent card for chromatography reaction.

[0075] The other specific implementation methods in this embodiment are the same as those in Embodiment 1, and will not be described in detail hereafter.

[0076] Example 4

[0077] like Figure 4 As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0078] In this embodiment, the sample collection tube 20 and the sample processing tube 10 are integrated. Specifically, the sample pretreatment tube in this embodiment includes a second large-diameter section located at the top and a second small-diameter section located at the bottom, with the inner diameter of the second large-diameter section being larger than the inner diameter of the second small-diameter section. Thus, the second large-diameter section is the sample processing tube 10 in this embodiment, and the second small-diameter section is the sample collection tube 20 in this embodiment.

[0079] In this embodiment, the bottom of the sample collection tube 20 is provided with a puncture port 23 for the aspiration needle to puncture and aspirate the sample, and a puncture membrane 24 is provided inside the puncture port 23. After the aspiration needle punctures the puncture membrane 24, it enters the sample collection tube 20 through the puncture port 23 to aspirate the pretreatment sample solution, so that the pretreatment sample solution can be transferred to the chromatography reagent card for chromatography reaction.

[0080] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0081] The sample preprocessing method of this embodiment includes the following steps:

[0082] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0083] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0084] The sample chromatography method of this embodiment includes the following steps:

[0085] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0086] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0087] Specifically, in this embodiment, after obtaining the pretreated sample solution, the sampling needle pierces the puncture membrane 24 and enters the sample collection tube 20 through the puncture port 23 to draw the pretreated sample solution. Then, the sampling needle is used to transfer the pretreated sample solution to the chromatography reagent card for chromatography reaction.

[0088] The other specific implementation methods in this embodiment are the same as those in Embodiment 1, and will not be described in detail hereafter.

[0089] Example 5

[0090] like Figure 5 As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0091] In this embodiment, the sealing element 11 is a sealing piston that fits tightly with the sample processing tube 10, and the sealing piston keeps the liquid inlet 11 sealed. When it is necessary to add diluent and whole blood sample into the sample processing tube 10, the sealing piston is removed and the diluent and whole blood sample are added; after the diluent and whole blood sample are added, the sealing piston is inserted into the liquid inlet 11 to maintain the sealed state inside the sample processing tube 10; after the diluent and whole blood sample are mixed to obtain the sample solution, the sealing piston is driven to move along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorbent material 14.

[0092] In this embodiment, the upper part of the sample collection tube 20 is fitted over the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are in a sealed fit, with an axial limiting fit between them. In this embodiment, after obtaining the pre-treated sample solution, the sample processing tube 10 can be removed from the sample collection tube 20. At this time, the opening of the sample collection tube 20 is open, and the pre-treated sample can be aspirated by inserting a sampling needle into the sample collection tube 20. Then, the pre-treated sample solution is transferred to the chromatography reagent card for chromatography reaction using the sampling needle.

[0093] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0094] The sample preprocessing method of this embodiment includes the following steps:

[0095] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0096] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0097] In this embodiment, after the diluent and whole blood sample are mixed and a sample solution is obtained, the driving sealing piston moves along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorption material 14, increasing the speed at which the sample solution passes through the adsorption material 14, and thus improving efficiency.

[0098] The sample chromatography method of this embodiment includes the following steps:

[0099] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0100] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0101] Other specific implementation methods in this embodiment are the same as in Embodiment 1, and will not be described in detail here.

[0102] Example 6

[0103] like Figure 6 As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0104] In this embodiment, the sealing element 11 is a sealing piston that fits tightly with the sample processing tube 10, and the sealing piston keeps the liquid inlet 11 sealed. When it is necessary to add diluent and whole blood sample into the sample processing tube 10, the sealing piston is removed and the diluent and whole blood sample are added; after the diluent and whole blood sample are added, the sealing piston is inserted into the liquid inlet 11 to maintain the sealed state inside the sample processing tube 10; after the diluent and whole blood sample are mixed to obtain the sample solution, the sealing piston is driven to move along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorbent material 14.

[0105] In this embodiment, the upper part of the sample collection tube 20 is fitted over the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are sealed together. An axial limiting structure is provided between the sample collection tube 20 and the sample processing tube 10. In this embodiment, the bottom of the sample collection tube 20 is provided with a puncture port 23 for aspiration needle puncture, and a puncture membrane 24 is provided inside the puncture port 23. After the aspiration needle punctures the puncture membrane 24, it enters the sample collection tube 20 through the puncture port 23 to aspirate the pre-treated sample solution, facilitating the transfer of the pre-treated sample solution to the chromatography reagent card for chromatography reaction.

[0106] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0107] The sample preprocessing method of this embodiment includes the following steps:

[0108] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0109] In this embodiment, after the diluent and whole blood sample are mixed and a sample solution is obtained, the driving sealing piston moves along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorption material 14, increasing the speed at which the sample solution passes through the adsorption material 14, and thus improving efficiency.

[0110] The sample chromatography method of this embodiment includes the following steps:

[0111] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0112] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0113] Specifically, in this embodiment, after obtaining the pretreated sample solution, the sampling needle pierces the puncture membrane 24 and enters the sample collection tube 20 through the puncture port 23 to draw the pretreated sample solution. Then, the sampling needle is used to transfer the pretreated sample solution to the chromatography reagent card for chromatography reaction.

[0114] The other specific implementation methods in this embodiment are the same as those in Embodiment 2, and will not be described in detail hereafter.

[0115] Example 7

[0116] like Figure 7As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0117] In this embodiment, the sealing element 11 is a sealing piston that fits tightly with the sample processing tube 10, and the sealing piston keeps the liquid inlet 11 sealed. When it is necessary to add diluent and whole blood sample into the sample processing tube 10, the sealing piston is removed and the diluent and whole blood sample are added; after the diluent and whole blood sample are added, the sealing piston is inserted into the liquid inlet 11 to maintain the sealed state inside the sample processing tube 10; after the diluent and whole blood sample are mixed to obtain the sample solution, the sealing piston is driven to move along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorbent material 14.

[0118] In this embodiment, the upper part of the sample collection tube 20 is fitted over the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are in a sealed fit. In this embodiment, the sample processing tube 10 can move axially relative to the sample collection tube 20. Specifically, a lower piston 16 is fitted over the lower end of the sample processing tube 10, and the sample collection tube 20 and the sample processing tube 10 are in a sealed fit through the lower piston 16. The bottom of the sample collection tube 20 is provided with a bottom outlet 25, which is covered with a sealing film 26. Thus, after the pretreatment sample solution is collected in the sample collection tube 20, the sealing film 26 can be torn open, driving the sample processing tube 10 to move axially downward relative to the sample collection tube 20. Under the action of the lower piston 16, the pretreatment sample solution in the sample collection tube 20 is driven to flow out from the bottom outlet 25 and transferred to the chromatography reagent card for chromatography reaction.

[0119] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0120] The sample preprocessing method of this embodiment includes the following steps:

[0121] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0122] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0123] In this embodiment, after the diluent and whole blood sample are mixed and a sample solution is obtained, the driving sealing piston moves along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorption material 14, increasing the speed at which the sample solution passes through the adsorption material 14, and thus improving efficiency.

[0124] The sample chromatography method of this embodiment includes the following steps:

[0125] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0126] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0127] Specifically, in this embodiment, after obtaining the pre-treated sample solution, the sealing film 26 is torn open, and the sample processing tube 10 is driven to move axially downward relative to the sample collection tube 20. Under the action of the lower piston 16, the pre-treated sample solution in the sample collection tube 20 is driven to flow out from the bottom outlet 25 and transferred to the chromatography reagent card for chromatography reaction.

[0128] The other specific implementation methods in this embodiment are the same as those in Embodiment 3, and will not be described in detail hereafter.

[0129] Example 8

[0130] like Figure 8 As shown, the sample pretreatment tube of this embodiment includes a sample processing tube 10 and a sample collection tube 20. Specifically, the top of the sample processing tube 10 is provided with a liquid inlet 11 and a sealing member 12 for sealing the liquid inlet 11. The bottom of the sample processing tube 10 is provided with a liquid outlet 13. The sample collection tube 20 is located below the sample processing tube 10 and is used to collect the pretreated sample liquid flowing out from the liquid outlet 13.

[0131] In this embodiment, the sealing element 11 is a sealing piston that fits tightly with the sample processing tube 10, and the sealing piston keeps the liquid inlet 11 sealed. When it is necessary to add diluent and whole blood sample into the sample processing tube 10, the sealing piston is removed and the diluent and whole blood sample are added; after the diluent and whole blood sample are added, the sealing piston is inserted into the liquid inlet 11 to maintain the sealed state inside the sample processing tube 10; after the diluent and whole blood sample are mixed to obtain the sample solution, the sealing piston is driven to move along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorbent material 14.

[0132] In this embodiment, the sample collection tube 20 and the sample processing tube 10 are integrated. Specifically, the sample pretreatment tube in this embodiment includes a second large-diameter section located at the top and a second small-diameter section located at the bottom, with the inner diameter of the second large-diameter section being larger than the inner diameter of the second small-diameter section. Thus, the second large-diameter section is the sample processing tube 10 in this embodiment, and the second small-diameter section is the sample collection tube 20 in this embodiment.

[0133] In this embodiment, the bottom of the sample collection tube 20 is provided with a puncture port 23 for the aspiration needle to puncture and aspirate the sample, and a puncture membrane 24 is provided inside the puncture port 23. After the aspiration needle punctures the puncture membrane 24, it enters the sample collection tube 20 through the puncture port 23 to aspirate the pretreatment sample solution, so that the pretreatment sample solution can be transferred to the chromatography reagent card for chromatography reaction.

[0134] The following describes the specific implementation of the sample pretreatment method and sample chromatography method using the sample pretreatment tube of this embodiment.

[0135] The sample preprocessing method of this embodiment includes the following steps:

[0136] After opening the seal 11, add the diluent and whole blood sample into the sample processing tube 10 in sequence, then install the seal 11 at the liquid inlet 11, mix the diluent and whole blood sample to obtain the sample solution.

[0137] During and after the sample solution is mixed, the red blood cells in the sample solution are adsorbed by the adsorbent material 14, and the remaining liquid enters the sample collection tube 20 to obtain the pre-treated sample solution.

[0138] In this embodiment, after the diluent and whole blood sample are mixed and a sample solution is obtained, the driving sealing piston moves along the sample processing tube 10 toward the sample collection tube 20, driving the sample solution to flow through the adsorption material 14, increasing the speed at which the sample solution passes through the adsorption material 14, and thus improving efficiency.

[0139] The sample chromatography method of this embodiment includes the following steps:

[0140] Step 1: Obtain the pretreated sample solution using the sample pretreatment method described above in this embodiment;

[0141] Step 2: Transfer the pretreatment sample solution to the chromatography reagent card for chromatography reaction.

[0142] Specifically, in this embodiment, after obtaining the pretreated sample solution, the sampling needle pierces the puncture membrane 24 and enters the sample collection tube 20 through the puncture port 23 to draw the pretreated sample solution. Then, the sampling needle is used to transfer the pretreated sample solution to the chromatography reagent card for chromatography reaction.

[0143] The other specific implementation methods in this embodiment are the same as those in Embodiment 4, and will not be described in detail hereafter.

[0144] The above-described embodiments are merely preferred embodiments provided to fully illustrate the present invention, and the scope of protection of the present invention is not limited thereto. Equivalent substitutions or modifications made by those skilled in the art based on the present invention are all within the scope of protection of the present invention. The scope of protection of the present invention is defined by the claims.

Claims

1. A sample pretreatment tube, characterized in that: Includes sample processing tubes and sample collection tubes; The sample processing tube has a liquid inlet at the top and a seal for sealing the liquid inlet; the sample processing tube has an outlet at the bottom; the sample collection tube is located below the sample processing tube and is used to collect the pre-processed sample liquid flowing out from the outlet; the sample processing tube is equipped with an adsorbent material for adsorbing red blood cells.

2. The sample pretreatment tube according to claim 1, characterized in that: The sample processing tube contains a drying agent, which is located above the adsorbent material.

3. The sample pretreatment tube according to claim 2, characterized in that: The drying treatment material is disposed on the inner wall of the sample processing tube.

4. The sample pretreatment tube according to claim 1, characterized in that: The adsorption material is a blood filtration membrane or ultrafine glass fiber.

5. The sample pretreatment tube according to claim 1, characterized in that: The sealing element is a sealing cap covering the liquid inlet; or, the sealing element is a sealing piston that fits tightly with the sample processing tube.

6. The sample pretreatment tube according to claim 1, characterized in that: The sample collection tube has a puncture port at the bottom for aspiration needle, and a puncture membrane is provided inside the puncture port; or, the sample collection tube has a bottom outlet at the bottom, and a sealing membrane is provided over the bottom outlet.

7. The sample pretreatment tube according to claim 1, characterized in that: The sample collection tube is equipped with a marker.

8. The sample pretreatment tube according to any one of claims 1-7, characterized in that: The upper part of the sample collection tube is fitted over the sample processing tube, and the sample collection tube and the sample processing tube are sealed together.

9. The sample pretreatment tube according to claim 8, characterized in that: The sample collection tube and the sample processing tube are axially constrained; or, the sample processing tube can move relative to the sample collection tube in the axial direction.

10. The sample pretreatment tube according to any one of claims 1-7, characterized in that: The sample collection tube and the sample processing tube are integrated into one unit.

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