Plant tissue culture bottle capable of automatically supplementing liquid

Abstract

The utility model relates to the technical field of plant tissue culture, in particular to a plant tissue culture bottle capable of automatically supplementing liquid, which comprises a culture bottle with a top opening, the top of the culture bottle is detachably connected with a liquid storage tank, the bottom of the liquid storage tank is fixedly communicated with a liquid outlet pipe, and the liquid outlet pipe extends into the culture bottle and is provided with a buoyancy plugging component; the buoyancy plugging assembly comprises a connecting ring fixed in the liquid outlet pipe, a supporting disc is concentrically arranged in the connecting ring, the supporting disc and the connecting ring are connected through a plurality of fixing rods, a movable rod is slidably arranged in the supporting disc in a penetrating mode, a limiting plate is fixed to the top of the movable rod, and a floating ball is fixed to the bottom of the movable rod. The diameter of the floating ball is larger than the inner diameter of the liquid outlet pipe and is made of a foam material; the device is reasonable in structural design, convenient to operate and capable of automatically and accurately supplementing liquid and maintaining balance of culture liquid in real time.

Description

TECHNICAL FIELD

[0001] The utility model relates to plant tissue culture technical field especially relates to a plant tissue culture bottle of automatic liquid supplement. BACKGROUND

[0002] In the plant tissue culture process, the nutrient solution in the culture bottle will gradually reduce along with the absorption and evaporation of the plant, and needs to be supplemented regularly by artificial, otherwise it will affect the plant growth.

[0003] But the traditional culture bottle has the following problems:

[0004] First, the cover needs to be opened frequently to add nutrient solution, which increases the risk of pollution.

[0005] Second, artificial supplement is difficult to control accurately, which affects the culture effect.

[0006] Third, it depends on artificial observation, which may lead to delayed liquid supplement. UTILITY MODEL CONTENT

[0007] The utility model solves the main technical problem, provides a plant tissue culture bottle of automatic liquid supplement, which has reasonable structure design, convenient operation, can automatically and accurately supplement liquid, and can maintain the balance of culture solution in real time.

[0008] To solve the above technical problems, the utility model provides the following technical scheme:

[0009] A plant tissue culture bottle of automatic liquid supplement, comprising a culture bottle with an open top, a liquid storage tank is detachably connected to the top of the culture bottle, a liquid outlet pipe is fixedly connected to the bottom of the liquid storage tank, the liquid outlet pipe extends into the culture bottle and is provided with a buoyancy blocking assembly; the buoyancy blocking assembly comprises a connecting ring fixed in the liquid outlet pipe, a support disc is concentrically arranged in the connecting ring, the support disc and the connecting ring are connected by a plurality of fixed rods, a movable rod is slidably arranged in the support disc, a limiting plate is fixed to the top of the movable rod, and a floating ball is fixed to the bottom of the movable rod; the floating ball has a diameter greater than the inner diameter of the liquid outlet pipe and is made of foam material.

[0010] The utility model further optimizes the above technical scheme as follows:

[0011] A plurality of air vents are formed in the upper side wall of the culture bottle, and a microporous filter membrane is covered and fixed on each air vent.

[0012] Further optimization: a liquid adding pipe is fixedly and communicatively connected to the top of the liquid storage tank, and a sealing cover is threadedly connected to the liquid inlet of the liquid adding pipe.

[0013] Further optimization: a circular groove is formed in the bottom of the liquid storage tank, an internal thread is arranged on the inner side wall of the groove, an external thread matched with the internal thread is arranged on the outer wall of the mouth of the culture bottle, and the liquid storage tank and the culture bottle are connected by threads.

[0014] Further optimization: A sealing gasket is attached to the bottom of the groove. The sealing gasket includes a washer bottom, and an avoidance hole is opened on the washer bottom to avoid the liquid outlet pipe.

[0015] Further optimization: The bottom edge of the washer is provided with an inner ring protrusion and an outer ring protrusion arranged at intervals, and the height of the inner ring protrusion is greater than the height of the outer ring protrusion.

[0016] Further optimization: The inner diameter of the inner ring protrusion is smaller than the inner diameter of the culture bottle mouth and the outer diameter is larger than the inner diameter of the culture bottle mouth; the outer diameter of the outer ring protrusion is larger than the outer diameter of the culture bottle mouth and the inner diameter is smaller than the outer diameter of the culture bottle mouth.

[0017] Further optimization: The inner wall of the inner ring protrusion is a vertical annular surface, and the outer wall is an arc-shaped surface extending towards the bottom of the washer; the outer wall of the outer ring protrusion is a vertical surface, and the inner wall is an inclined surface extending towards the bottom of the washer.

[0018] Further optimization: A pressure part is provided between the inner ring protrusion and the outer ring protrusion. The thickness of the pressure part is less than the height of the inner ring protrusion and the outer ring protrusion, but greater than the thickness of the bottom of the washer.

[0019] Further optimization: The surface of the float is coated with a hydrophobic coating to prevent liquid adhesion from affecting buoyancy performance.

[0020] In this invention, the buoyancy sealing component uses a foam float that moves a movable rod as the liquid level rises and falls. When the liquid level in the culture bottle drops, the float sinks and opens the outlet, allowing the storage tank to replenish the liquid. When the liquid level rises again, the float rises and re-seals the outlet, thus achieving dynamic balance of the liquid level, reducing manual intervention, and ensuring that the plant tissue is always in the optimal culture environment.

[0021] With the above technical solution, the present invention has a reasonable structural design, is easy to operate, and can automatically and accurately replenish liquid and maintain the balance of culture medium in real time.

[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model;

[0025] Figure 2It is a structure schematic view of the floating plugging assembly in the embodiment of the utility model.

[0026] Figure 3 It is a structure schematic view of the liquid storage tank in the embodiment of the utility model.

[0027] Figure 4 It is a structure schematic view of the sealing gasket in the embodiment of the utility model.

[0028] In the figure: 1-culture bottle; 2-liquid storage tank; 3-liquid outlet pipe; 4-buoyancy plugging assembly; 41-connection ring; 42-supporting disc; 43-fixed rod; 44-movable rod; 45-limiting plate; 46-float ball; 5-vent hole; 6-micro porous filter membrane; 7-liquid adding pipe; 8-sealing cover; 9-groove; 10-sealing gasket; 101-gasket bottom; 102-avoidance hole; 103-inner ring protruding part; 104-outer ring protruding part; 105-pressurizing part; 11-anti-skid base. DETAILED DESCRIPTION

[0029] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0030] As shown in the figure, Figures 1-4 An automatic liquid supplementing plant tissue culture bottle comprises a culture bottle 1 with an open top, a liquid storage tank 2 is detachably connected to the top of the culture bottle 1, a liquid outlet pipe 3 is fixedly connected to the bottom of the liquid storage tank 2 and extends into the culture bottle 1, and a buoyancy plugging assembly 4 is arranged at the liquid outlet end of the liquid outlet pipe 3.

[0031] The buoyancy plugging assembly 4 comprises a connection ring 41 fixedly installed in the liquid outlet pipe 3, a supporting disc 42 concentrically arranged in the connection ring 41, a plurality of fixed rods 43 connecting the supporting disc 42 and the connection ring 41 together, a movable rod 44 slidingly arranged in the supporting disc 42, a limiting plate 45 fixedly connected to the top of the movable rod 44, and a float ball 46 fixedly connected to the bottom of the movable rod 44, wherein the diameter of the float ball 46 is greater than that of the liquid outlet pipe 3, the float ball 46 is made of foam material, and the float ball 46 drives the movable rod 44 to open and close the liquid outlet according to the change of the liquid level.

[0032] In this way, firstly, the buoyancy plugging assembly 4 drives the movable rod 44 to move according to the change of the liquid level through the float ball 46 made of foam material, when the liquid level in the culture bottle 1 decreases, the float ball 46 sinks to open the liquid outlet, and the liquid storage tank 2 supplements liquid; after the liquid level rises, the float ball 46 floats to reseal the liquid outlet. This design realizes the dynamic balance of the liquid level, reduces manual intervention, and ensures that the plant tissue is always in the best culture environment.

[0033] Secondly, the combination of the connecting ring 41, the support disc 42 and the fixing rod 43 not only ensures the smoothness of the liquid outlet pipe 3, but also provides a stable sliding track for the movable rod 44; meanwhile, the cooperation of the floating ball 46 and the limiting plate 45 prevents the assembly from disengaging, the lightweight design of the foam material improves the sensitivity, and the overall structure is durable and easy to disassemble and maintain.

[0034] A plurality of air vents 5 are formed on the upper side of the outer side wall of the culture bottle 1, and a microporous filter membrane 6 is covered and fixed on the inner side wall of each air vent 5.

[0035] In this way, first, the plurality of air vents 5 cover the microporous filter membrane 6 to ensure the free exchange of oxygen and carbon dioxide, effectively prevent microbial contamination, and maintain the sterility of the culture environment, especially suitable for long-term or sensitive plant tissue culture.

[0036] Secondly, the air vents 5 are evenly distributed on the upper side of the side wall, avoiding local gas concentration differences, reducing the risk of culture liquid blockage, and adapting to oscillation or tilting operation, improving the consistency of plant tissue growth.

[0037] Thirdly, the filter membrane is directly fixed in the air vent 5, which is not easy to fall off or seepage, and has good air permeability and sealing performance, which simplifies the design and reduces the cost, and is suitable for high-throughput or special culture scenes.

[0038] The microporous filter membrane used in the embodiment is a standardized product commercially purchased, and its working principle, use method and installation method are all known to those skilled in the art, so they will not be described here.

[0039] The top of the liquid storage tank 2 is fixedly connected with a liquid adding pipe 7, and a sealing cover 8 is threadedly connected to the liquid inlet of the liquid adding pipe 7.

[0040] In this way, the sealing cover 8 connected by threads can ensure the airtightness of the liquid inlet of the liquid adding pipe 7, prevent liquid leakage or external pollution, and facilitate quick opening and closing to realize safe and efficient liquid adding operation, improve the convenience and reliability of the liquid storage process.

[0041] The bottom of the liquid storage tank 2 is provided with a circular groove 9, the inner side wall of the groove 9 is provided with an internal thread, and the outer wall of the mouth of the culture bottle 1 is provided with an external thread matched with the internal thread, and the liquid storage tank 2 and the culture bottle 1 are detachably connected through the internal thread and the external thread.

[0042] In this way, first, through the precise cooperation of the internal and external threads, the liquid storage tank 2 and the culture bottle 1 are connected tightly, effectively preventing liquid leakage or external pollutants from entering, and maintaining the cleanliness and safety of the culture environment.

[0043] Secondly, the screw design enables the culture bottle 1 to be quickly screwed or disassembled, facilitating individual replacement, cleaning or sterilization, and improving the flexibility and efficiency of the experimental or production process.

[0044] In the present embodiment, the internal thread and the external thread adopt standard bottle mouth threads, and the thread profile is trapezoidal or triangular.

[0045] In this way, the standardized threaded interface can achieve universal adaptation of different culture bottles 1 and liquid storage tanks 2, reducing system complexity.

[0046] The depth of the groove 9 is 5-15 mm, and the diameter of the groove 9 is 0.5-2 mm larger than the outer diameter of the bottle mouth of the culture bottle 1.

[0047] The bottom of the groove 9 is pasted with a sealing gasket 10 to form a double-sealing structure when the liquid storage tank 2 is connected to the culture bottle 1.

[0048] In the present embodiment, the sealing gasket 10 is made of elastic sealing material, preferably silicone rubber, ethylene propylene diene rubber (EPDM) or polytetrafluoroethylene (PTFE) material, to ensure good sealing performance, corrosion resistance and long-term stability.

[0049] In the present embodiment, the sealing gasket 10 is made of silicone rubber, ethylene propylene diene rubber (EPDM) or polytetrafluoroethylene (PTFE) material. These materials are conventional sealing materials in the field, and their physical and chemical properties and sealing mechanisms are well known to those skilled in the art, so they will not be described here.

[0050] The sealing gasket 10 includes a gasket bottom 101, and the gasket bottom 101 is provided with a relief hole 102 for avoiding the liquid outlet pipe 3.

[0051] The gasket bottom 101 is provided with an inner ring protruding portion 103 and an outer ring protruding portion 104 arranged at a distance on one side edge, and the height of the inner ring protruding portion 103 is greater than that of the outer ring protruding portion 104.

[0052] The inner diameter of the inner ring protruding portion 103 is smaller than the inner diameter of the bottle mouth, and the outer diameter is greater than the inner diameter of the bottle mouth. The outer diameter of the outer ring protruding portion 104 is greater than the outer diameter of the bottle mouth, and the inner diameter is smaller than the outer diameter of the bottle mouth.

[0053] The inner side wall of the inner ring protruding portion 103 is a vertical annular surface, and the outer side wall is an arc surface extending to the gasket bottom 101. The outer side wall of the outer ring protruding portion 104 is a vertical surface, and the inner side wall is an inclined surface extending to the gasket bottom 101.

[0054] The inner ring protruding portion 103 and the outer ring protruding portion 104 are provided with a pressurizing portion 105 therebetween, and the thickness of the pressurizing portion 105 is smaller than the height of the inner ring protruding portion 103 and the outer ring protruding portion 104, and greater than the thickness of the gasket bottom 101.

[0055] In this way, firstly, the inner ring protruding part 103 and the outer ring protruding part 104 are designed with different heights and diameters, and are tightly fitted with the inner and outer sides of the bottle mouth respectively, the vertical annular surface of the inner ring protruding part 103 enhances the radial sealing, and the inclined surface of the outer ring protruding part 104 cooperates with the pressurizing part 105 to further strengthen the axial compression sealing, effectively preventing gas penetration and ensuring sealing reliability.

[0056] Secondly, the arc surface of the inner ring protruding part 103 and the inclined surface of the outer ring protruding part 104 enable the sealing gasket 10 to adapt to different sizes or slightly deformed bottle mouths, and fill the small gaps through deformation under pressure, thereby improving the fault tolerance to manufacturing errors or installation deviations, and reducing the stringent requirements on processing precision.

[0057] Thirdly, the avoidance hole 102 precisely matches the outlet pipe 3 to avoid installation interference, and the pressurizing part 105 is arranged to uniformly transmit the pressure to the inner and outer protruding parts when the bottle cap is screwed, thereby reducing local wear and tear, simplifying the installation operation, and balancing the sealing durability and use convenience.

[0058] The surface of the floating ball 46 is coated with a hydrophobic coating to prevent liquid adhesion from affecting the buoyancy performance.

[0059] In this embodiment, the floating ball 46 is made of lightweight closed-cell foam material, preferably polyethylene (PE) foam or polyurethane (PU) foam, which meets the conventional technical requirements in terms of density, buoyancy characteristics and water resistance. The application of such materials in buoyancy devices is well known and specific physical property parameters are not described here.

[0060] The culture bottle 1 and the liquid storage tank 2 are both made of transparent material.

[0061] In this way, firstly, the transparent material allows direct observation of the growth of plant tissues (such as callus formation and root development), real-time monitoring of contamination or browning, timely adjustment of culture conditions, and reduction of contamination risk caused by opening inspection.

[0062] Secondly, the transparent bottle body allows natural light or artificial light source to penetrate uniformly, meeting the light period requirements of certain plant tissues (such as light-induced differentiation), while avoiding local overexposure or insufficient light, and improving the culture efficiency.

[0063] Thirdly, the transparent design facilitates the recording of morphological changes of plant tissues at different growth stages (such as bud point germination and organogenesis).

[0064] In this embodiment, the transparent material of the culture bottle 1 and the liquid storage tank 2 is polycarbonate or polystyrene.

[0065] In the embodiment, the culture bottle 1 and the liquid storage tank 2 are made of polycarbonate (PC) or polystyrene (PS) transparent material. The two materials have excellent optical transparency, chemical stability and mechanical strength, and are mature materials commonly used in the field of cell culture containers. The material properties and processing technology are well known to those skilled in the art, and thus will not be described.

[0066] The culture bottle 1 is provided with an anti-skid base 11 at the bottom, and the anti-skid base 11 is provided with anti-skid lines at the bottom.

[0067] In this way, first, the anti-skid base 11 and the lines can prevent the bottle from sliding or falling accidentally.

[0068] In the embodiment, the anti-skid lines 14 are concentric circle lines or grid lines.

[0069] In the embodiment, the anti-skid base 11 is made of elastic rubber material, preferably natural rubber (NR), styrene-butadiene rubber (SBR) or silicone rubber, and the friction coefficient and damping performance meet the conventional technical requirements in the field. The selection of the material can effectively enhance the stability of the equipment placement, and the material properties and processing technology are well known to those skilled in the art, so specific parameters will not be described.

[0070] For those skilled in the art, according to the teachings of the present application, changes, modifications, replacements and variations of the embodiments without departing from the principles and spirits of the present application still fall within the protection scope of the present application.

Claims

1. An automatic liquid supplementing plant tissue culture bottle comprising a top open culture bottle (1), characterized in that: The culture bottle (1) top detachable connection has liquid storage tank (2), liquid storage tank (2) bottom fixed communication has liquid outlet pipe (3), liquid outlet pipe (3) extends to culture bottle (1) and is equipped with buoyancy blocking assembly (4); the buoyancy blocking assembly (4) includes the connecting ring (41) fixed in the liquid outlet pipe (3), the concentric support disc (42) is equipped in the connecting ring (41), and the support disc (42) is connected with the connecting ring (41) through multiple fixed rods (43), the movable rod (44) is slidably arranged in the support disc (42), the movable rod (44) top is fixed with the limiting plate (45), and the bottom is fixed with the float ball (46), the float ball (46) diameter is greater than the inner diameter of the liquid outlet pipe (3) and is made of foam material.

2. The self-replenishing plant tissue culture bottle of claim 1, wherein: The culture bottle (1) side wall is provided with a plurality of air holes (5) above, and each air hole (5) is covered and fixed with a microporous filter membrane (6).

3. The self-replenishing plant tissue culture bottle of claim 1, wherein: The top of the liquid storage tank (2) is fixedly connected with a liquid adding pipe (7), and the liquid inlet of the liquid adding pipe (7) is threadedly connected with a sealing cover (8).

4. The self-replenishing plant tissue culture bottle of claim 1, wherein: The bottom of the liquid storage tank (2) is provided with a circular groove (9), and the inner wall of the groove (9) is provided with an internal thread. The outer wall of the bottle mouth of the culture bottle (1) is provided with an external thread matched with the internal thread. The liquid storage tank (2) and the culture bottle (1) are connected through threads.

5. The self-replenishing plant tissue culture bottle of claim 4, wherein: The bottom of the groove (9) is pasted with a sealing gasket (10), and the sealing gasket (10) includes a gasket bottom (101), and the gasket bottom (101) is provided with an avoiding hole (102) avoiding the liquid outlet pipe (3).

6. The self-replenishing plant tissue culture bottle of claim 5, wherein: The inner circle protruding part (103) and the outer circle protruding part (104) are arranged at the one side edge of the gasket bottom (101), and the height of the inner circle protruding part (103) is greater than that of the outer circle protruding part (104).

7. The self-replenishing plant tissue culture bottle of claim 6, wherein: The inner diameter of the inner circle protruding part (103) is less than the inner diameter of the bottle mouth of the culture bottle (1), and the outer diameter is greater than the inner diameter of the bottle mouth of the culture bottle (1). The outer diameter of the outer circle protruding part (104) is greater than the outer diameter of the bottle mouth of the culture bottle (1), and the inner diameter is less than the outer diameter of the bottle mouth of the culture bottle (1).

8. The self-replenishing plant tissue culture bottle of claim 7, wherein: The inner side wall of the inner circle protruding part (103) is a vertical annular surface, and the outer side wall is an arc surface extending to the gasket bottom (101). The outer side wall of the outer circle protruding part (104) is a vertical surface, and the inner side wall is an inclined surface extending to the gasket bottom (101).

9. The self-replenishing plant tissue culture bottle of claim 8, wherein: The inner circle protruding part (103) and the outer circle protruding part (104) are provided with a pressurizing part (105), and the thickness of the pressurizing part (105) is less than the height of the inner circle protruding part (103) and the outer circle protruding part (104), and greater than the thickness of the gasket bottom (101).

10. The self-replenishing plant tissue culture bottle of claim 1, wherein: The surface of the float ball (46) is coated with a hydrophobic coating to prevent liquid adhesion from affecting the buoyancy performance.

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