Orifice plate and extraction equipment
By designing the end face of the sample loading hole to be higher than the top surface of the frame, the problem of difficult film removal in existing perforated plates is solved, enabling efficient film removal operations for both manual and automated equipment, and improving the utilization efficiency of the perforated plate.
Patent Information
- Application Number
- CN202423239578.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2026-02-27
- Estimated Expiration
- 2034-12-27
AI Technical Summary
The reagent well openings of existing well plates are flush with the top of the plate body, which increases the difficulty of peeling off the membrane. Manual and automated membrane peeling is inefficient, and there is a lack of equipment adapted for automated membrane peeling.
The end face of the sample feeding hole is designed to be higher than the top surface of the frame, forming a certain height difference, so as to facilitate the film tearing operation by manual and automated equipment such as cutting tools.
It combines the convenience of manual film tearing with the high efficiency of automated film tearing equipment, improving operational efficiency and adapting to the application of various automated equipment.
Smart Images
Figure CN223950984U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to molecular biology experiment equipment technical field, concretely relates to a well plate and extraction equipment. BACKGROUND
[0002] With the application of biological technology more and more widely, more and more operations on biological specimens, such as protein / nucleic acid detection or purification, can now be completed quickly through corresponding commercial well plates.
[0003] The well plate, usually with a pre-set corresponding reagent hole, has added corresponding reagents, including liquid and / or powder, in at least part of the sample wells when it leaves the factory. Then the top (opening of the sample well) uses a sealing film made of chemically inert material, which at least needs to be physically or chemically inert with the reagents in the sample well, usually made of high molecular plastic material, such as PE (polyethylene) film, PVC (polyethylene) film, etc., covering and pasting to the hole of each sample well, sealing the sample well to prevent the reagents inside the sample well from leaking, to facilitate carrying and transportation. And some have a layer of aluminum film pasted outside this layer of inert material film. When in use, the operator needs to tear open the sealing film on the top of the sample well to expose the hole of each sample well, allowing the corresponding sample well to load reagents and / or samples to be tested to perform the corresponding biochemical operation.
[0004] In this way, when using the well plate, the operator usually needs to open the pasted sealing film first.
[0005] However, the current well plate has the opening of the reagent hole flush with the top surface of the well plate body, resulting in less remaining film being compressed for the same film compression specification, making it more difficult to tear the film, which requires the operator to spend extra time manually tearing the film when in use. It can be seen that the manual film tearing process caused by the flush of the reagent hole of the well plate with the top surface of the well plate body is very inconvenient, inefficient and not conducive to rapid detection.
[0006] In addition, in order to adapt to automatic production, it is also necessary to be able to automatically tear the film, and currently there is no reagent kit suitable for automatic film tearing.
[0007] Therefore, there is an urgent need for a well plate suitable for manual and automatic film tearing. Utility model content
[0008] In order to solve the above technical problems, the utility model provides a well plate, the orifice end surface of the sample adding hole is higher than the top surface of the frame body, because the orifice end surface of the sample adding hole and the top surface of the frame body have a certain height, therefore under the same film pressing specification, when the well plate is sealed with film, a larger number of unpressed sealing films are formed around, which is easy to tear by hand and can be applied to various automatic equipment (such as cutting tools) for cutting and tearing the film.
[0009] The utility model provides a well plate, comprising:
[0010] The frame body and the sample adding hole;
[0011] The sample adding hole is arranged on the frame body, and the orifice end surface of the sample adding hole is higher than the top surface of the frame body.
[0012] In a specific embodiment, the height of the orifice end surface higher than the top surface of the frame body is greater than 1mm, preferably, the height is 1mm-10mm, further, the height is 3mm-5mm.
[0013] In a specific embodiment, the sample adding hole is multiple, and the multiple sample adding holes are connected or not connected.
[0014] In a specific embodiment, the sample adding hole is any one of 24 holes, 48 holes, 96 holes or 384 holes, or
[0015] The well plate comprises at least one large capacity hole and at least two standard holes arranged in sequence along a first direction, wherein the volume of the large capacity hole is greater than the volume of one standard hole.
[0016] Preferably, the well plate comprises 8 rows of at least one large capacity hole and at least two standard holes arranged in sequence along the first direction.
[0017] In a specific embodiment, the length of the large capacity hole is equal to the length of at least two standard holes connected without obstacles, preferably, the length of the large capacity hole in the first direction is equal to the length of three standard holes connected without obstacles, or the length of the large capacity hole in the first direction is M times the length of a standard hole, and the length of the large capacity hole in the second direction is N times the length of a standard hole; M and N are positive integers, M is greater than or equal to 2, preferably, M is greater than or equal to 3, N is greater than or equal to 2, and the first direction is orthogonal to the second direction.
[0018] In a specific embodiment, the bottom of the large capacity hole comprises a first arc-shaped part recessed from the inside to the outside, a second arc-shaped part connected to the two sides of the first arc-shaped part, and a third arc-shaped part connected to the two second arc-shaped parts, and the connecting line formed by the highest point and the lowest point of the first arc-shaped part, the second arc-shaped part and the third arc-shaped part is a gentle transition curve.
[0019] In one specific embodiment, the bottom center of the standard hole is provided with an arc-shaped smooth recess, preferably a semispherical recess.
[0020] In one specific embodiment, a reinforcing member is further provided between the frame and the sample loading hole.
[0021] In one specific embodiment, the frame, the reinforcing member and the sample loading hole are integrally formed.
[0022] The utility model embodiment further provides an extraction equipment, including the magnetic bar of adsorbing magnetic beads from the large capacity hole, the magnetic bar sleeve is provided on the magnetic bar, the height, the quantity of magnetic bar sleeve correspond with the large capacity hole setting.
[0023] The utility model has the advantages of the following:
[0024] The utility model embodiment provides a kind of hole plate, comprising: frame and sample loading hole, sample loading hole is arranged on frame, and the orifice end surface of sample loading hole is higher than frame top surface.Due to the orifice end surface of sample loading hole is higher than frame top surface, there is a height difference between orifice end surface and frame top surface, so under the same film gauge, when hole plate is sealed, a circle of more un-tightened sealing film is formed in periphery, this structure design not only makes the operation of manual film tearing more convenient, and benefit from this height difference, various automation equipment (such as cutting tool) also can be efficiently applied in hole plate film tearing process.
[0025] Further, height range is preferably 3mm-5mm, can be adapted to the specification height of most cutting tools on market, to ensure the versatility and convenience of hole plate in film tearing process. BRIEF DESCRIPTION OF DRAWINGS
[0026] In order to more clearly illustrate the technical scheme of the utility model, the following will be to the embodiment or prior art description needed to use the drawing briefly introduced, obviously, the following description in the drawing is only some embodiments of the utility model, for those skilled in the art, under the premise of not paying creative labor, other drawings can also be obtained according to these drawings.
[0027] Figure 1 It is the three-dimensional structure schematic diagram of the hole plate 100 provided by the utility model;
[0028] Figure 2 It is the three-dimensional structure schematic diagram of another hole plate 200 provided by the utility model;
[0029] Figures 3-4 It is the cross-sectional structure schematic diagram of the hole plate 100 provided by the utility model;
[0030] Figure 5 Another cross-sectional structure schematic view of the hole plate 200 is provided in the utility model;
[0031] Figure 6 Another structure schematic view of the hole plate 100 from another perspective is provided in the utility model;
[0032] In the drawing:
[0033] 1: frame body; 2: sample hole; 3: reinforcing member;
[0034] 11: top surface; 12: identification part; 21: orifice end face;
[0035] 22: large capacity hole; 23: standard hole; 24: sample pool; 230: recess;
[0036] 220: first arc-shaped part; 221: second arc-shaped part; 222: third arc-shaped part;
[0037] 240: first arc-shaped part; 241: second arc-shaped part; 242: third arc-shaped part; DETAILED DESCRIPTION
[0038] The utility model provides a kind of hole plate and extraction equipment, to make the purpose, technical scheme and effect of the utility model of the utility model more clear, definite, the following referring to the drawing and taking example to the utility model is further detailedly explained.It should be understood that the specific embodiment described herein is merely used to explain the utility model, and is not used to limit the utility model.
[0039] Therefore, the detailed description of the embodiment of the utility model provided in the drawing below is not intended to limit the scope of the claimed utility model, but only represents selected embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by those of ordinary skill in the art without creative labor are within the scope of protection of the utility model.
[0040] It should be noted that: similar labels and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.
[0041] In the description of the utility model, it needs to be explained that the directions or position relations of the terms "bottom", "middle", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like are based on the directions or position relations shown in the drawings, or the directions or position relations of the utility model product when it is usually placed, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the utility model. In addition, the terms "first", "second", "third" and the like are only used for differentiation in description, and cannot be understood as indicating or implying relative importance.
[0042] Figure 1 The utility model provides a kind of orifice plate 100, including frame 1 and sample addition hole 2, sample addition hole 2 is arranged on frame 1, and the orifice end surface 21 of sample addition hole 2 is higher than the top surface 11 of frame 1.
[0043] In the use of orifice plate 100, first need to open orifice plate, since the orifice end surface 21 of sample addition hole 2 is higher than the top surface 11 of frame 1, i.e. the orifice end surface 21 of sample addition hole 2 and the top surface 11 of frame 1 have height difference h, therefore, under the same film gauge, when orifice plate is sealed, a circle of more un-tightened sealing film is formed around, this structure design not only makes the operation of manual film tearing more convenient, and thanks to this height difference, various automation equipment (such as cutting tool) can also be efficiently applied in orifice plate film tearing process.
[0044] In order to make orifice plate 100 when applicable to various automation equipment, for example orifice plate film tearing machine, cutting tool, such as cutting knife, easy to cut the height part between the orifice end surface 21 of sample addition hole 2 and the top surface 11 of frame 1, the height h of the orifice end surface higher than the top surface 11 of frame 1 needs to be greater than 1mm, for example, in some embodiments, the height range is 1mm-10mm, in other embodiments, in order to be able to adapt to the height of most cutting tool specifications on the market, thereby ensuring the versatility and convenience of orifice plate in film tearing process, the height range can also be 3mm-5mm, the height range between the orifice end surface 21 of sample addition hole 2 and the top surface 11 of frame 1 can be set according to specific application scenarios, and the embodiments of the application do not limit this.
[0045] According to the needs of specific application scenarios, sample addition hole 2 can be multiple, and multiple sample addition holes can be connected or not connected.
[0046] It needs to be explained that although Figure 1 The shape of sample addition hole is shown, Figure 1 It is only for example, that is, Figure 1The sample loading wells in this application shall not be limited to the sample loading wells in the embodiments of this application. For example, in some specific embodiments, the sample loading wells may be any one of 24 wells, 48 wells, 96 wells or 384 wells.
[0047] In other specific embodiments, in order to accommodate a larger volume of sample solution, the sample well 2 may further include at least one large-capacity well 22 and at least two standard wells 23 arranged sequentially along a first direction, such as the x-direction, wherein the volume of the large-capacity well is greater than the volume of one standard well. See [link to relevant documentation]. Figure 1 and Figure 2 .
[0048] In one specific embodiment, the perforated plate 1 is provided with at least one large-capacity hole and at least two standard holes arranged sequentially in eight rows along a first direction, such as the x-direction. See [link to previous embodiment]. Figure 1 .
[0049] In a preferred embodiment, each row includes two sets of sample loading wells. Each set of sample loading wells includes, for example, large-capacity wells, two washing wells, and one elution well arranged sequentially. The large-capacity wells are used to contain lysis buffer, the washing wells are used to contain washing buffer, and the elution wells are used to contain purification buffer. The well plate 1 in this embodiment can also be modified from a conventional 96-well plate. In existing 96-well plates, all sample loading wells are standard wells arranged in a 12*8 matrix, with 12 standard wells per row. Typically, these are divided into two groups of six standard wells each, arranged sequentially. During modification, it is only necessary to completely open up the first few, for example, the first three standard wells in each group to form large-capacity wells.
[0050] In another specific embodiment, see Figure 2 To accommodate a larger volume of sample solution, the well plate 1 is provided with two sample loading chambers 24 (large capacity wells) and corresponding standard wells. The length of the sample loading chamber 24 in the first direction, such as the x-direction, is M times the length of the standard well, and the length of the sample loading chamber 24 in the second direction, such as the y-direction, is N times the length of the standard well. M and N are positive integers, M≥2, preferably M≥3 and N≥2, and the first and second directions are orthogonal. For example, in a more preferred embodiment, the length of the sample loading chamber 24 in the first direction, such as the x-direction, is 3 times the length of the standard well, and the length of the sample loading chamber in the second direction, such as the y-direction, is 8 times the length of the standard well. In this embodiment, the well plate 1 can also be modified from the existing 96-well plate. All the sample loading wells in the existing 96-well plate are standard wells, arranged in a 12*8 matrix with 12 standard wells in each row. They are usually divided into two groups with 6 standard wells in each group, arranged sequentially. When modifying, it is only necessary to open up the first few, for example, the first three standard wells in each group in the first direction, and open up some or all (for example, 4 or 8 rows) of the 8 rows of standard wells in the second direction to form a sample loading pool.
[0051] SeeFigures 3-4 In order to make the well plate more stable when placed on the base (not shown), the bottom of the large-volume well 22 includes a first arc-shaped recess 220 that is concave from the inside to the outside, a second arc-shaped recess 221 connected to the two sides of the first arc-shaped recess 220, and a third arc-shaped recess 222 connected to the two second arc-shaped recesses 221, and the connecting line formed by the highest point and the lowest point of the first arc-shaped recess 220, the second arc-shaped recess 221 and the third arc-shaped recess 222 is a smooth transition curve. The central part of the bottom of the standard well 23 is provided with an arc-shaped smooth recess 230 to facilitate the precipitation of the sample liquid, and the shape of the recess 230 may, for example, be hemispherical, and of course the recess 230 can also be of other shapes.
[0052] Referring to Figure 5 When the large-volume well is a sample addition pool 24, in order to make the well plate 200 more stable when placed on the base (not shown), the bottom of the sample addition pool 24 also includes a first arc-shaped recess 240 that is concave from the inside to the outside, a second arc-shaped recess 241 connected to the two sides of the first arc-shaped recess 240, and a third arc-shaped recess 242 connected to the two second arc-shaped recesses 241, and the connecting line formed by the highest point and the lowest point of the first arc-shaped recess 240, the second arc-shaped recess 241 and the third arc-shaped recess 242 is a smooth transition curve.
[0053] In order to reduce the probability of thermal shrinkage holes occurring during the injection molding of the well plate, at the same time to prevent the well plate from deforming, and to more stably clamp the well plate on the base, a reinforcing member 3 is further provided between the frame 1 and the sample addition well 2, which may, for example, be a reinforcing rib or the like, as shown in Figure 3 .
[0054] In order to facilitate the carrying and operation of the well plate, the frame 1, the sample addition well 2 and the reinforcing member 3 can be integrally formed.
[0055] In order to quickly position the well plate in the up-down and left-right directions, a marking portion 12 can also be provided at the corners of the frame 1, wherein the marking portion 12 can be a cut corner, the marking portion 12 can also be provided on the top surface 11 of the frame 1, and the marking portion 12 can also be some pattern sprayed.
[0056] In order to facilitate the movement and transportation of the well plate and the standardized extraction, the well plate 100 further includes a sealing film layer such as an aluminum foil film, which is used for the sample addition well. Since there is a height difference between the orifice end face of the sample addition well and the top surface of the frame, when the well plate is sealed, a larger number of un-tightened sealing films will be formed around the well plate under the same film pressing specification. This structural design not only makes the manual film tearing operation more convenient, but also benefits from the height difference, so that various automatic equipment (such as cutting tools) can also be efficiently applied to the well plate film tearing process.
[0057] The utility model embodiment further provides an extraction equipment (not shown), including from the big capacity hole adsorbs the magnetic bead of magnetic bar, the magnetic bar is sleeved with the magnetic bar cover, in order to make the magnetic bar cover still can contact the bottom of the hole plate, the height, quantity of magnetic bar cover need and big capacity hole corresponding arrangement.
[0058] In conclusion, the utility model embodiment provides a kind of hole plate and extraction equipment, comprising: frame and sample addition hole, sample addition hole is arranged on frame, and the orifice end surface of sample addition hole is higher than the top surface of frame. Because the orifice end surface of sample addition hole is higher than the top surface of frame, there is a height difference between orifice end surface and the top surface of frame, so under the same film gauge, when hole plate is sealed, a circle of more un-tightened sealing film is formed around, this structure design not only makes the operation of manual film tearing more convenient, and benefit from this height difference, various automation equipment (such as cutting tool) can also be efficiently applied in hole plate film tearing process.
[0059] The above description has fully disclosed the specific embodiments of the utility model. It should be pointed out that any modification made by skilled person in the specific embodiments of the utility model does not deviate from the scope of the claims of the utility model. Correspondingly, the scope of the claims of the utility model is not limited to the foregoing specific embodiments.
Claims
1. An orifice plate, characterized by The application relates to a frame and a sample adding hole. The sample adding hole is arranged on the frame, and the hole opening end surface of the sample adding hole is higher than the top surface of the frame. The sample adding hole is any one of 24 holes, 48 holes, 96 holes or 384 holes. The frame comprises at least one large capacity hole and at least two standard holes arranged in sequence along a first direction. The volume of the large capacity hole is larger than that of one standard hole. The height of the hole opening end surface of the sample adding hole is greater than 1 mm.
2. The well plate of claim 1, wherein, The sample adding hole is a plurality of sample adding holes, and the plurality of sample adding holes are connected or not connected.
3. The well plate of claim 1 wherein, The sample adding hole comprises at least one large capacity hole and at least two standard holes arranged in sequence along a first direction.
4. The well plate of claim 3, wherein, The length of the large capacity hole is equal to the length of the at least two standard holes connected without obstacles.
5. The well plate of claim 4, wherein, The bottom of the large capacity hole comprises a first arc-shaped part recessed from the inside to the outside, a second arc-shaped part connected with the two sides of the first arc-shaped part, and a third arc-shaped part connected with the two second arc-shaped parts, and the connecting line formed by the highest point and the lowest point of the first arc-shaped part, the second arc-shaped part and the third arc-shaped part is a gentle transition curve.
6. The well plate of claim 4, wherein, The bottom of the standard hole is provided with an arc-shaped smooth recess in the center.
7. The well plate of claim 4, wherein The application further relates to a reinforcing part arranged between the frame and the sample adding hole.
8. The well plate of any one of claims 1-7, wherein, The frame, the reinforcing part and the sample adding hole are integrally formed.
9. The well plate of claim 8, wherein, The application further relates to a magnetic rod for adsorbing magnetic beads in the large capacity hole, and a magnetic rod sleeve is arranged on the magnetic rod, and the height and the number of the magnetic rod sleeve correspond to the large capacity hole.
10. An extraction apparatus, characterized by