Manual test tube culture medium split charging device

Abstract

The utility model relates to the field of culture medium split charging, and discloses a manual test tube culture medium split charging device, which comprises a soft plastic liquid containing bottle, a test tube, a test tube and a test tube, and is characterized in that the soft plastic liquid containing bottle is used for containing a liquid culture medium; the hard plastic bottle cap is in threaded sealing connection with a bottle opening of the soft plastic liquid containing bottle, the liquid outlet pipe is arranged on the hard plastic bottle cap in a penetrating mode and integrally formed with the hard plastic bottle cap, the hard plastic bottle cap is in threaded sealing connection with the bottle opening of the soft plastic liquid containing bottle, and the liquid outlet pipe is arranged on the hard plastic bottle cap in a penetrating mode. The liquid outlet pipe comprises a liquid outlet section protruding out of the hard plastic bottle cap and a connecting section arranged in the soft plastic liquid containing bottle, the liquid outlet section is arranged in a bent arc shape, the inner diameter of the liquid outlet section is gradually reduced from 1-1.5 cm to 0.2-0.3 cm of the inner diameter of a liquid outlet, and the inner diameter of the connecting section is 1-1.5 cm; and the soft pipette is sleeved on the connecting section of the liquid outlet pipe, and the soft pipette extends to the bottom of the soft plastic liquid containing bottle. The split charging device is simple in structure, low in cost and convenient to operate.

Description

Technical Field

[0001] This utility model relates to the field of culture medium dispensing, and in particular to a manual test tube culture medium dispensing device. Background Technology

[0002] In edible fungi production and research, tissue isolation, mycelial culture, and mother culture preservation all require the use of test tube slant culture media. The preparation of test tube culture media involves dispensing a specific amount of melted liquid culture medium into test tubes, sterilizing them, and then cooling them on slant incubators. Currently, there are three main methods for dispensing test tube culture media: First, the traditional method, directly pouring the liquid culture medium from a beaker into the test tubes. This method, if not performed skillfully, can easily result in culture medium contaminating the test tube opening, posing a risk of later contamination by other microorganisms. Second, using a syringe to dispense the culture medium. This method greatly reduces the risk of contamination at the test tube opening, but it can only dispense a few test tubes at a time, requiring repeated aspiration and injection of the medium, making the operation cumbersome and inefficient. Third, using an automated culture medium dispenser. This method allows for precise control of the liquid addition and is highly efficient, but the equipment is expensive. It is suitable for companies or large-scale production of test tube culture media, but not for small-scale individual businesses or production of small quantities of test tube culture media. Utility Model Content

[0003] The technical problem to be solved by this utility model is to overcome the deficiencies and defects mentioned in the background art above, and to provide a manual test tube culture medium dispensing device that is simple in structure, low in cost, and easy to operate. To solve the above technical problem, the technical solution proposed by this utility model is as follows:

[0004] A manual test tube culture medium dispensing device is used for dispensing fluid culture medium containing agar. It consists of a flexible plastic dispensing bottle, an integrally molded rigid plastic cap and dispensing tube, and a flexible suction tube.

[0005] Flexible plastic bottles for holding liquid culture media;

[0006] The rigid plastic bottle cap is threadedly sealed to the mouth of the flexible plastic liquid bottle. The liquid outlet tube passes through the rigid plastic bottle cap and is integrally formed with the rigid plastic bottle cap. The liquid outlet tube includes a liquid outlet section protruding from the rigid plastic bottle cap and a connecting section disposed inside the flexible plastic liquid bottle. The liquid outlet section is arranged in a curved arc, and the inner diameter of the liquid outlet section gradually decreases from 1-1.5cm to the inner diameter of the liquid outlet being 0.2cm~0.3cm. The inner diameter of the connecting section is 1-1.5cm.

[0007] A flexible suction tube is fitted onto the connecting section of the dispensing tube, and extends to the bottom of the flexible plastic liquid bottle.

[0008] In one embodiment, the curvature of the liquid outlet section is 100-110 degrees.

[0009] In one embodiment, the flexible plastic liquid bottle and flexible suction tube are made of low-density polyethylene or ethylene-vinyl acetate copolymer.

[0010] In one embodiment, the rigid plastic bottle cap and the dispensing tube are made of high-density polyethylene or polypropylene.

[0011] In one embodiment, the inner diameter of the soft suction tube is 1-1.6 cm.

[0012] Compared with existing technologies, the advantages of this invention are as follows: The aforementioned manual test tube culture medium dispensing device includes a soft plastic bottle, an integrally molded hard plastic cap, and a dispensing tube. The dispensing tube gradually curves and extends after emerging from the bottle mouth, and the inward-extending dispensing port effectively prevents contamination of the test tube opening. The inner diameter of the initial part of the dispensing section (the section connecting to the inside of the bottle mouth) is 1-1.5 cm, and the inner diameter of the ending part (the dispensing port) is 0.2-0.3 cm. The dispensing section is arranged in a curved arc, and the inner diameter of the dispensing section gradually decreases from 1-1.5 cm to 0.2-0.3 cm at the dispensing port. The inner diameter of the connecting section is 1-1.5 cm. This design helps to increase pressure, and because the soft plastic bottle is made of soft plastic, test tube culture medium of a certain viscosity can be easily squeezed out. Multiple test tubes can be continuously dispensed without the culture medium cooling and solidifying, thus improving work efficiency. The outlet section is designed with a curved arc, which allows the outlet tube to extend deep into the front of the test tube during the addition of culture medium, thus avoiding the culture medium from contaminating the test tube opening and reducing the possibility of contamination. Attached Figure Description

[0013] Figure 1 A side view of a manual test tube culture medium dispensing device according to one embodiment;

[0014] Figure 2 This is a diagram showing the usage status of a manual test tube culture medium dispensing device according to one embodiment.

[0015] Reference numerals: 10, soft plastic bottle; 20, hard plastic cap; 30, dispensing tube; 32, dispensing section; 34, connecting section; 40, soft suction tube. Detailed Implementation

[0016] To facilitate understanding of this utility model, the following description will be provided in more comprehensive and detailed manner with reference to the accompanying drawings and preferred embodiments. However, the scope of protection of this utility model is not limited to the following specific embodiments.

[0017] like Figure 1-2As shown, one embodiment of a manual test tube culture medium dispensing device is used to dispense fluid culture medium (with a certain viscosity) containing agar. It consists of a soft plastic bottle 10, an integrally formed rigid plastic cap 20, a dispensing tube 30, and a soft suction tube 40. The soft plastic bottle 10 is used to hold the liquid culture medium. The rigid plastic cap 20 is threadedly sealed to the mouth of the soft plastic bottle 10. The dispensing tube 30 passes through the rigid plastic cap 20. The dispensing tube 30 and the rigid plastic cap are integrally formed from the same material to avoid the inability to form a sealed connection during use. The dispensing tube 30 includes a dispensing section 32 protruding from the rigid plastic bottle cap 20 and a connecting section 34 disposed within the flexible plastic liquid bottle 10. The dispensing section 32 is curved and arc-shaped, with its inner diameter gradually decreasing from 1-1.5 cm to 0.2 cm-0.3 cm at the dispensing opening. The connecting section 34 has an inner diameter of 1-1.5 cm. A flexible suction tube 40 is fitted onto the connecting section 34 of the dispensing tube 30, extending to the bottom of the flexible plastic liquid bottle 10. The flexible suction tube 40 has an inner diameter of 1-1.6 cm. The inner diameter of the flexible suction tube is slightly larger than or the same as the outer diameter of the connecting section, utilizing its flexibility to fit onto the connecting section 34. Because the soft plastic bottle 10 is made of soft plastic, it can easily squeeze out test tube culture media of a certain viscosity. The diameter of the dispensing tube 30 is designed to gradually decrease from the inside of the bottle to the dispensing outlet, and the inner diameter of the dispensing outlet is designed to increase pressure. At the same time, the dispensing outlet is not easily blocked by culture media with a certain viscosity. The above-mentioned manual test tube culture media dispensing device dispenses smoothly. The sealed environment after the hard plastic bottle cap 20 reduces heat loss and prevents the culture media from solidifying before it is completely dispensed. It can continuously dispense multiple test tubes without cooling and solidifying the culture media, improving work efficiency. For example, a 500mL soft plastic bottle 10 can continuously dispense about 30 test tubes. The dispensing section 32 is designed with a curved arc, which allows the dispensing tube 30 to be inserted into the front of the test tube during the addition of culture media, avoiding culture media from contaminating the test tube mouth and reducing the possibility of contamination. Preferably, the curvature of the dispensing section 32 is 100-110 degrees. Specifically, in one embodiment, the soft plastic liquid bottle 10 and the soft liquid suction tube 40 are made of low-density polyethylene or ethylene-vinyl acetate copolymer, and the hard plastic bottle cap 20 and the liquid outlet tube 30 are made of high-density polyethylene or polypropylene.

[0018] In one specific embodiment, the flexible plastic liquid bottle 10 is cylindrical, with a height of 15cm, a bottom diameter of 7cm, and a maximum liquid capacity of 500ml. The flexible suction tube 40 has an inner diameter of 1cm and a length of 14cm. The dispensing tube 30 extends from inside the bottle to the outside and then gradually bends, with the bend forming a 100° arc. The inner diameter of the starting part of the dispensing tube 30 (the part connecting the bottle mouth and the suction tube) is 1cm, and the inner diameter of the ending part (the dispensing port) is 0.2cm. The inner diameter of the middle section of the dispensing tube 30 gradually decreases from 1cm to 0.2cm as the tube extends.

[0019] The aforementioned manual test tube culture medium dispensing device includes a soft plastic bottle 10, an integrally molded hard plastic cap 20, and a dispensing tube 30. The dispensing tube 30 gradually curves and extends after exiting the bottle mouth, with an inwardly extending outlet effectively preventing contamination of the test tube opening. The inner diameter of the initial part (connecting section 34 to the inside of the bottle mouth) of the dispensing section 32 of the dispensing tube 30 is 1-1.5 cm, and the inner diameter of the ending part (dispensing outlet) is 0.2-0.3 cm. The dispensing section 32 is curved and arc-shaped, with its inner diameter gradually decreasing from 1-1.5 cm to 0.2-0.3 cm at the dispensing outlet. The inner diameter of the connecting section 34 is 1-1.5 cm. This design facilitates increased pressure, and because the soft plastic bottle 10 is made of soft plastic, it can easily squeeze out test tube culture medium of a certain viscosity. This allows for continuous dispensing of multiple test tubes without the culture medium cooling and solidifying, improving work efficiency. The outlet section 32 is designed with a curved arc, which allows the outlet section 32 of the outlet tube 30 to extend into the front of the test tube during the addition of culture medium, thus avoiding the culture medium from contaminating the mouth of the test tube and reducing the possibility of contamination.

[0020] The above description is merely a preferred embodiment of this utility model, and the protection scope of this utility model is not limited to the above embodiments. For those skilled in the art, improvements and modifications obtained without departing from the technical concept of this utility model should also be considered within the protection scope of this utility model.

Claims

1. A manual test tube medium aliquoting device for aliquoting fluidic medium containing agar, characterized by, The liquid bottle is composed of a soft plastic liquid bottle, a hard plastic bottle cap and a liquid outlet pipe, and a soft liquid suction pipe. The soft plastic liquid bottle is used for containing liquid medium. The hard plastic bottle cap is threadedly connected to the bottle mouth of the soft plastic liquid bottle. The liquid outlet pipe is arranged on the hard plastic bottle cap and integrally formed with the hard plastic bottle cap.

2. The manual test tube media dispensing device of claim 1, wherein: The soft liquid suction pipe is sleeved on the connecting section of the liquid outlet pipe and extends to the bottom of the soft plastic liquid bottle.

3. The manual test tube media dispensing device of claim 1, wherein: The bending arc of the liquid outlet section is 100-110 degrees.

4. The manual test tube media dispensing device of claim 1, wherein: The material of the soft plastic liquid bottle and the soft liquid suction pipe is low-density polyethylene or ethylene-vinyl acetate copolymer.

5. The manual test tube media dispensing device of claim 1, wherein: The material of the hard plastic bottle cap and the liquid outlet pipe is high-density polyethylene or polypropylene. The inner diameter of the soft liquid suction pipe is 1-1.6 cm.

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