Portable field LAMP (loop-mediated isothermal amplification) device for quantitatively detecting pathogenic bacteria

Abstract

The utility model relates to the technical field of spectrum detection devices, and discloses a portable field LAMP (Loop-Mediated Isothermal Amplification) quantitative pathogenic bacterium detection device. Comprising an LAMP spectrum detection chamber and a photoelectric signal processing electric box, a pair of concave square fixing grooves are formed in the central axis of the bottom of the LAMP spectrum detection chamber; a light source chamber and a signal receiving chamber are arranged on the left side and the right side of the central axis in a mirror symmetry manner; a halogen tungsten bulb, a monochromator and an optical collimation system are arranged in the light source chamber, and a photoelectric tube is arranged in the optical signal receiving chamber; a photoelectric signal amplification module and a signal processing module are arranged in the photoelectric signal processing electric box; and the photoelectric tube is in signal connection with the signal processing module after passing through the photoelectric signal amplification module. According to the utility model, the successful application of the LAMP quantitative detection technology in the field is realized, so that the early detection and the drug resistance monitoring of pathogens are realized, and the scientific and accurate drug application is further realized.

Description

Technical Field

[0001] This invention belongs to the field of spectral detection device technology, specifically relating to a portable field LAMP device for quantitative detection of pathogens. Background Technology

[0002] Crop diseases cause yield losses in years of widespread outbreaks, and early detection and resistance monitoring of major crop diseases are crucial for disease control and a prerequisite for the scientific and precise use of pesticides. Traditional pathogen detection required laboratory testing, making online field testing impossible and time-consuming. However, the newly developed loop-mediated isothermal amplification PCR technology allows for rapid, efficient, and specific amplification of target DNA under isothermal conditions. It has been successfully applied to plant pathogen identification and pesticide resistance detection, facilitating timely adjustments to pesticide application strategies, improving pesticide efficacy, and reducing pesticide usage.

[0003] The isothermal environment required for isothermal amplification PCR (LAMP) detection is mainly provided by laboratory water baths or PCR instruments. Existing water baths and PCR instruments require fixed power supplies, are bulky, inconvenient to move, and unsuitable for providing an isothermal environment in the field. Patent CN 210481352 U, entitled "A Device for Providing Reaction Conditions for LAMP Detection of Plant Pathogens in the Field," includes a constant temperature treatment chamber and a mobile power supply box connected from top to bottom. It provides a small, portable, energy-efficient device that provides reaction conditions for LAMP detection without requiring a fixed power supply. However, this device lacks quantitative detection capabilities; it can only observe the color differences of the reactants with the naked eye, making it impossible to accurately determine the quantity of pathogens, which is detrimental to pesticide science and precise application. Therefore, improvements to existing technologies are needed. Utility Model Content

[0004] The technical problem to be solved by this invention is to provide a portable field LAMP device for quantitative detection of pathogens that is simple in structure and easy to use.

[0005] To solve the above-mentioned technical problems, this utility model provides a portable field LAMP quantitative detection device for pathogens, including a constant temperature treatment box, a mobile power supply box, a LAMP spectral detection chamber, and a photoelectric signal processing box;

[0006] A pair of recessed square fixing slots are provided on the central axis of the bottom of the LAMP spectral detection chamber. A first inner cover plate and a second inner cover plate are mirrored on the left and right sides of the central axis. The first inner cover plate and the second inner cover plate, together with the box body of the LAMP spectral detection chamber, form a light source chamber and a signal receiving chamber, respectively. Two gaps are opened face to face on the first inner cover plate and the second inner cover plate. The gaps on the first inner cover plate, the square fixing slots and the gaps on the second inner cover plate are located on the same straight line.

[0007] The light source chamber contains a halogen tungsten bulb, a monochromator, and an optical collimation system, while the optical signal receiving chamber contains a phototube;

[0008] The photoelectric signal processing box is equipped with a photoelectric signal amplification module and a signal processing module. The phototube is connected to the signal processing module after passing through the photoelectric signal amplification module.

[0009] As an improvement to the portable field LAMP quantitative detection device for pathogens of this invention:

[0010] In the LAMP spectral detection chamber, the four lower walls protrude inward to form a mounting platform. The first inner cover plate and the second inner cover plate are L-shaped, and the transverse arm sides of the first inner cover plate and the second inner cover plate are fixedly connected to the top surface of the mounting platform.

[0011] As a further improvement to the portable field LAMP quantitative detection device for pathogens of this invention:

[0012] The LAMP spectral detection chamber is equipped with a top cover, which is hinged to the LAMP spectral detection chamber.

[0013] As a further improvement to the portable field LAMP quantitative detection device for pathogens of this invention:

[0014] The halogen tungsten bulb, phototube photoelectric signal amplification module, and signal processing module are all electrically connected to the DC power interface on the mobile power supply box.

[0015] As a further improvement to the portable field LAMP quantitative detection device for pathogens of this invention:

[0016] The LAMP spectral detection chamber is located above the photoelectric signal processing box and is fixedly connected to it.

[0017] As a further improvement to the portable field LAMP quantitative detection device for pathogens of this invention:

[0018] The constant temperature processing chamber is connected to the LAMP spectral detection chamber via a snap-fit ​​mechanism.

[0019] As a further improvement to the portable field LAMP quantitative detection device for pathogens of this invention:

[0020] The gap is located on the vertical arm side of the first inner cover plate and the second inner cover plate, and the gap is rectangular.

[0021] The beneficial effects of this utility model are mainly reflected in:

[0022] This invention provides a portable device that requires no fixed power supply and can be quickly installed on the existing patent (patent number: CN 210481352U) "A device for providing reaction conditions for LAMP detection of plant pathogens in the field". After the reaction is complete, quantitative detection can be performed directly, achieving rapid and accurate pathogen detection. The aim is to realize the successful application of LAMP quantitative detection technology in the field, thereby achieving early detection of pathogens and monitoring of drug resistance, ultimately enabling scientific and precise pesticide application, delaying the emergence of drug-resistant strains, preventing the loss of varietal resistance, and reducing pesticide use and environmental pollution. This invention can reduce unnecessary pesticide use, is convenient to carry and use, and can be used for sample testing in the field or production site. Attached Figure Description

[0023] The specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings.

[0024] Figure 1 This is a schematic diagram of the structure of the LAMP spectral detection chamber and the photoelectric signal processing box of this utility model;

[0025] Figure 2 for Figure 1 Schematic diagram of the Zhongguang Optoelectronic Signal Processing Box;

[0026] Figure 3 This is a schematic diagram showing the usage status of the portable field LAMP quantitative detection device for pathogens according to this utility model. Detailed Implementation

[0027] The present invention will be further described below with reference to specific embodiments, but the scope of protection of the present invention is not limited thereto:

[0028] Example 1: A portable field LAMP device for quantitative detection of pathogens, such as... Figure 3 As shown, it includes a LAMP spectral detection chamber 1 and a photoelectric signal processing box 2. The LAMP spectral detection chamber 1 and the photoelectric signal processing box 2 can be installed on the existing patent (patent number: CN 210481352 U) "A device for providing reaction conditions for LAMP detection of plant pathogens in the field".

[0029] The existing patent (patent number: CN 210481352 U) "Apparatus for Providing Reaction Conditions for LAMP Detection of Plant Pathogens in the Field" includes a constant temperature treatment chamber and a mobile power supply box. A buckle for connecting to the LAMP spectral detection chamber 1 is added to one side of the constant temperature treatment chamber. A slot is provided on one outer wall of the LAMP spectral detection chamber 1. After the buckle on the constant temperature treatment chamber is inserted into the slot of the LAMP spectral detection chamber 1, the LAMP spectral detection chamber 1 and the constant temperature treatment chamber are connected and fixed together. Figure 3 As shown.

[0030] LAMP spectral detection chamber 1 is a rectangular box with an open top, such as Figure 2 As shown, a top cover 15 is provided at the top opening. The top cover 15 is connected to the LAMP spectral detection chamber 1 by a hinge and is used to shield the LAMP spectral detection chamber 1 from dust when used in the field. The bottom of the LAMP spectral detection chamber 1 is provided with a downward protruding step for engaging with the photoelectric signal processing box 2.

[0031] The lower half of the inner cavity of the LAMP spectral detection chamber 1 has a smaller cross-sectional area than the upper half, thus forming a mounting platform 14 protruding into the interior of the chamber on the four walls. Inside the LAMP spectral detection chamber 1, there are two L-shaped inner cover plates, a first inner cover plate 111 and a second inner cover plate 112, on the left and right sides respectively. The structure and shape of the first inner cover plate 111 and the second inner cover plate 112 are completely identical, and they are mirror images of each other relative to the central axis of the LAMP spectral detection chamber 1. The first inner cover plate 111 on the left and the chamber of the LAMP spectral detection chamber 1 form the light source chamber 11, and the second inner cover plate 112 on the right and the chamber of the LAMP spectral detection chamber 1 form the optical signal receiving chamber 12. Each of the four corners of the horizontal arm side of the first inner cover plate 111 and the second inner cover plate 112 has a screw hole, and the first inner cover plate 111 and the second inner cover plate 112 are fixedly connected to the top surface of the mounting platform 14 by screws. The first inner cover plate 111 and the second inner cover plate 112 each have two rectangular slits facing each other on the vertical arm sides, and the size of the slits is 1cm.

[0032] A pair of recessed square fixing grooves 13 are set on the central axis at the bottom of the LAMP spectral detection chamber 1 for placing and fixing cuvettes. The cuvettes contain the sample solution to be tested. Light of a fixed wavelength passes through the sample solution, and the sample solution will absorb or scatter the light, so the light passing through the cuvette will change.

[0033] The gap on the first inner cover plate 111 on the left, the square fixing groove 13 in the middle, and the gap on the second inner cover plate 112 on the right are on the same straight line, so that the light in the light source chamber 11 is emitted from the gap on the first inner cover plate 111 on the left, passes through the cuvette in the middle, and then enters the light signal receiving chamber 12 from the gap on the second inner cover plate 112 on the right.

[0034] The light source chamber 11 contains a halogen tungsten bulb, a monochromator, and an optical collimation system (lens). The halogen tungsten bulb emits broad-spectrum white light containing multiple wavelengths. To ensure that the sample in the cuvette receives only light of a specific wavelength, a monochromator is used to selectively filter out other wavelengths, allowing only the specific single wavelength to pass through. The optical collimation system is used to focus the light into a parallel beam, thereby accurately illuminating the cuvette. In this embodiment, the halogen tungsten bulb is the 721G model from Shanghai Bulb Factory No. 3. The existing patent (patent number: CN 210481352 U) "A device for providing reaction conditions for LAMP detection of plant pathogens in the field" has a DC power interface for the portable power supply box, and the halogen tungsten bulb is electrically connected to the DC power interface via a power cord to ensure that the halogen tungsten bulb operates under a stable voltage and avoid brightness fluctuations. The monochromator uses a Bohong Zhida BHNBF01-455 monochromatic filter.

[0035] The optical signal receiving chamber 12 is equipped with phototubes (GD-2A type, Jiangsu Yizheng Electron Tube Factory), located in the two gaps of the second inner cover plate 112, which are responsible for receiving photons.

[0036] The photoelectric signal processing box 2 is a rectangular box with a concave stepped top. There is an opening at each of the four corners of the concave step. The LAMP spectral detection chamber 1 is stacked on the photoelectric signal processing box 2. The convex bottom of the LAMP spectral detection chamber 1 and the concave top of the photoelectric signal processing box 2 engage with each other. Then, the LAMP spectral detection chamber 1 and the photoelectric signal processing box 2 are fixedly connected to each other by four hexagon socket screws.

[0037] The photoelectric signal processing box 2 is equipped with a photoelectric signal amplification module and a signal processing module. The phototube in the photoelectric signal receiving chamber 12 is connected to the signal processing module after passing through the photoelectric signal amplification module. The photoelectric signal amplification module is responsible for filtering and amplifying the electrical signal generated by the phototube, while the signal processing module is responsible for analog-to-digital conversion and data processing of the electrical signal, and outputting the calculated results. The temperature controller of the existing patent (patent number: CN 210481352 U) "A device for providing reaction conditions for LAMP detection of plant pathogens in the field" has a digital display. The signal processing module is connected to the digital display to display the calculation results of the signal processing module. The phototube, photoelectric signal amplification module, and signal processing module are all electrically connected to the DC power interface on the mobile power supply box to obtain the working power input. The specific usage method of this utility model is as follows:

[0038] After the latches on the constant temperature chamber are inserted into the slots of the LAMP spectral detection chamber 1, the LAMP spectral detection chamber 1 and the constant temperature chamber are connected and fixed together. The sample given in the centrifuge tube after reaction is poured into a 1cm cuvette and placed in the fixing slot 13. Light with a fixed wavelength is emitted from the light source chamber 11, shines on the cuvette through the gap of the first inner cover plate 111, and the light passing through the cuvette is received by the phototube in the light signal receiving chamber 12 after passing through the gap of the second inner cover plate 112, generating an electrical signal. The electrical signal is amplified by the photoelectric signal amplification module and then input to the signal processing module for processing. The results are displayed by the temperature controller with a digital display of the prior art patent (patent number: CN 210481352 U) "A device for providing reaction conditions for LAMP detection of plant pathogens in the field".

[0039] Finally, it should be noted that the above examples are merely a few specific embodiments of this utility model. Obviously, this utility model is not limited to the above embodiments and many variations are possible. All variations that can be directly derived or conceived by those skilled in the art from the disclosure of this utility model should be considered within the protection scope of this utility model.

Claims

1. A device for portable field LAMP quantitative detection of pathogenic bacteria, comprising a constant temperature treatment box and a mobile power supply box, characterized in that: It comprises a LAMP spectrum detection chamber (1) and a photoelectric signal processing electric box (2). A pair of concave square fixed grooves (13) are arranged on the middle axis of the bottom of the LAMP spectrum detection chamber (1), and a first inner cover plate (111) and a second inner cover plate (112) are symmetrically arranged on the left and right sides of the middle axis, and the first inner cover plate (111) and the second inner cover plate (112) each enclose a light source chamber (11) and a signal receiving chamber (12) with the box body of the LAMP spectrum detection chamber (1); two slits are respectively arranged on the first inner cover plate (111) and the second inner cover plate (112) in a face-to-face manner, and the slits on the first inner cover plate (111), the square fixed grooves (13) and the slits on the second inner cover plate (112) are located on the same straight line. A halogen tungsten bulb, a monochromator and an optical collimation system are arranged in the light source chamber (11), and a phototube is arranged in the light signal receiving chamber (12). A photoelectric signal amplification module and a signal processing module are arranged in the photoelectric signal processing electric box (2), and the phototube is connected with the signal processing module through the photoelectric signal amplification module.

2. The portable field LAMP quantitative detection pathogen device according to claim 1, wherein: In the LAMP spectrum detection chamber (1), the four walls of the lower part are inwardly convex to form a clamping table (14), the first inner cover plate (111) and the second inner cover plate (112) are L-shaped, and the lateral arms of the first inner cover plate (111) and the second inner cover plate (112) are fixedly connected with the top surface of the clamping table (14).

3. The portable field LAMP quantitative detection pathogen device according to claim 2, wherein: A top cover (15) is arranged on the top of the LAMP spectrum detection chamber (1), and the top cover (15) is hingedly connected with the LAMP spectrum detection chamber (1).

4. The portable field LAMP quantitative detection pathogen device according to claim 3, wherein: The halogen tungsten bulb, the phototube, the photoelectric signal amplification module and the signal processing module are all electrically connected with a direct current power supply interface on a mobile power supply box.

5. The portable field LAMP quantitative detection pathogen device according to claim 4, wherein: The LAMP spectrum detection chamber (1) is located above the photoelectric signal processing electric box (2) and is fixedly connected with the photoelectric signal processing electric box (2).

6. The portable field LAMP quantitative detection pathogen device according to claim 5, wherein: The constant temperature treatment box is connected with the LAMP spectrum detection chamber (1) through buckles.

7. The portable field LAMP quantitative detection pathogen device according to claim 6, wherein: The slits are arranged on the vertical arms of the first inner cover plate (111) and the second inner cover plate (112), and the slits are rectangular.

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