Active cell cryopreservation device for stem cell exosome

Abstract

The utility model provides an active cell cryopreservation device for stem cell exosomes, which relates to the technical field of stem cell exosomes and comprises a cryopreservation device body, a cover plate is arranged at the top of the cryopreservation device body, a controller is mounted on the right side of the front side of the top of the cryopreservation device body, a cell frame is arranged in the cryopreservation device body, and the cell frame is connected with the cover plate. According to the cell frame cryopreservation device, the cell frame is installed, specifically, a handle is pulled outwards to enable a positioning block to be separated from a positioning groove, a limiting block is rotated clockwise so that the cell frame can be stably placed in the cryopreservation device body through a vertical rod, the positioning block is loosened to be matched with the positioning groove to position the limiting block, and then the limiting block is rotated anticlockwise to limit the vertical rod; then, liquid nitrogen is released through operation of a controller to enter the cell frame, the temperature of the cell box is rapidly reduced to achieve cryopreservation, meanwhile, the liquid nitrogen release amount and temperature change are closely monitored through an observation window, and key parameters are recorded.

Description

TECHNICAL FIELD

[0001] The utility model relates to stem cell exosome technical field, concretely relates to a stem cell exosome active cell cryopreservation device. BACKGROUND

[0002] Exosome is a discoidal vesicle with a diameter of 40-100 nanometers, and belongs to one kind of extracellular vesicles, and stem cell exosome particularly refers to exosome secreted by stem cell, and the characteristics are that all cultured cell types can secrete exosome, and exosome naturally exists in body fluid, including blood, saliva, urine, cerebrospinal fluid and milk etc., and exosome contains complex RNA and protein and some functional characteristics of stem cell, two, origin and secretion process, origin: the origin, synthesis and secretion of stem cell exosome experience a series of complex processes, first, the cell membrane of mother cell forms early intracellular vesicle through endocytosis or "inward budding", gradually matures into late intracellular vesicle and multivesicular body, and the rudiment of exosome exists in the form of intraluminal vesicle inside multivesicular body, then fuses with cell membrane, and is discharged outside cell to become exosome in exocytosis, secretion process: the secretion process of exosome includes endocytosis, early intracellular vesicle formation, late intracellular vesicle, multivesicular body and final exocytosis discharge.

[0003] Active cell refers to cell with self-renewal ability, high reproduction repair and multi-directional differentiation potential, and these cells play an important role in human body, can continuously divide and differentiate into various different types of cells to maintain normal physiological function and structure of human body.

[0004] Therefore, the existing cryopreservation device has the problems in the limiting design of cell rack, and the cell rack is easy to shake in the device due to the vibration or bump of external environment during transportation, and the shaking not only affects the stability of cell, but also can cause the impact between cell and inner wall of cryopreservation device. UTILITY MODEL CONTENT

[0005] The utility model provides a stem cell exosome active cell cryopreservation device solves the problem of the prior art.

[0006] To solve the above technical problems, the technical scheme of the utility model is as follows:

[0007] The utility model discloses an active cell cryopreservation device of stem cell exosome, including the cryopreservation device body, the top of cryopreservation device body is provided with the apron, the right side of the top front of cryopreservation device body is installed with the controller, the inside of cryopreservation device body is provided with the cell frame, the bottom center of cryopreservation device body inner wall is fixedly connected with the vertical rod, the cell frame is located the surface of vertical rod, the center of cell frame top is provided with the limit structure, the limit structure includes the limit groove, the limit groove is located the top of vertical rod surface, the inside of limit groove is provided with the limit block, the bottom of limit block is connected with the top of cell frame through the round rod, the left side of limit block is provided with the positioning slot, the inside of positioning slot is connected with the positioning block of sliding, the right side of positioning block is fixedly connected with the handle, the surface of positioning block is connected with the fixed block of sliding, the bottom of fixed block is fixedly connected with the top of cell frame, the inside of cell frame is provided with the adjusting structure.

[0008] Through the above technical scheme, through the setting of limit block, limit groove, positioning slot and positioning block, the cell frame can be limited, and the cell frame can be prevented from shaking during transportation.

[0009] Further, the adjusting structure includes an isolation plate, the surface of the isolation plate is slidably connected with an adjusting plate, the top of the adjusting plate is fixedly connected with a vertical block, the front surface of the vertical block is fixedly connected with a horizontal rod, and the surface of the horizontal rod is sleeved with a horizontal block.

[0010] Through the above technical scheme, through the setting of the adjusting structure, the space inside the cell frame can be adjusted conveniently, and multiple groups of cells can be placed.

[0011] Further, the top of the adjusting plate is provided with a moving groove, the vertical block is located in the moving groove, the left side of the adjusting plate is provided with a horizontal groove, and the horizontal block is located in the horizontal groove.

[0012] Through the above technical scheme, through the setting of the moving groove, the vertical block can be limited through the moving groove, and through the setting of the horizontal groove, the adjusting plate can be limited in cooperation with the horizontal block.

[0013] Further, the bottom of the cryopreservation device body is provided with a sealing groove, and the sealing groove is internally provided with a sealing strip.

[0014] Through the above technical scheme, through the setting of the sealing groove and the sealing strip, the gap between the apron and the cryopreservation device body can be sealed.

[0015] Further, the bottom of the cryopreservation device body is provided with a sealing groove, and the sealing groove is internally provided with a sealing strip.

[0016] Through the technical scheme, the four sides of the cell holder can be limited through the setting of the circular groove, and the handle positioning block can be reset through the setting of the damping rod.

[0017] Further, the surface of the horizontal rod is fixedly connected with a torsional spring, and the other end of the torsional spring is fixedly connected with the outside of the horizontal block.

[0018] Through the technical scheme, the horizontal block can be prevented from separating from the inside of the horizontal groove through the elasticity of the torsional spring.

[0019] Further, the two sides of the cryopreservation device body are provided with observation windows, and the periphery of the bottom of the cryopreservation device body is fixedly connected with anti-skid blocks.

[0020] Through the technical scheme, the inside of the cryopreservation device body can be observed by the user through the setting of the observation window, and the anti-skid work of the cryopreservation device body can be increased through the setting of the anti-skid blocks.

[0021] The above scheme of the utility model has at least the following beneficial effects:

[0022] 1. The utility model discloses a cell holder installation: pull the handle to the outside and make the positioning block separate from the positioning groove, rotate the limiting block clockwise to place the cell holder stably in the inside of the cryopreservation device body through the vertical rod, loosen the positioning block to position the limiting block with the positioning groove, then rotate the limiting block counterclockwise to limit the vertical rod, ensure the stability of the cell holder, then release liquid nitrogen into the inside of the cell holder through the controller operation, rapidly reduce the temperature of the cell box to realize cryopreservation, closely monitor the liquid nitrogen release amount and temperature change through the observation window, and record the key parameters, when the cell reaches the required cryopreservation state, close the controller, carefully take out the cell box and clean the cell holder and the cell holder for next use.

[0023] 2. The utility model discloses a cell holder installation: pull the handle to the outside and make the positioning block separate from the positioning groove, rotate the limiting block clockwise to place the cell holder stably in the inside of the cryopreservation device body through the vertical rod, loosen the positioning block to position the limiting block with the positioning groove, then rotate the limiting block counterclockwise to limit the vertical rod, ensure the stability of the cell holder, then release liquid nitrogen into the inside of the cell holder through the controller operation, rapidly reduce the temperature of the cell box to realize cryopreservation, closely monitor the liquid nitrogen release amount and temperature change through the observation window, and record the key parameters, when the cell reaches the required cryopreservation state, close the controller, carefully take out the cell box and clean the cell holder and the cell holder for next use. DRAWINGS

[0024] Figure 1 It is the active cell cryopreservation device structure schematic view of the stem cell exosome of the utility model;

[0025] Figure 2 It is the cryopreservation device body section view schematic view of the utility model;

[0026] Figure 3 It is the cell holder schematic view of the utility model;

[0027] Figure 4It is the vertical rod schematic diagram of the utility model;

[0028] Figure 5 It is the Figure 3 Enlarged view of position A in the middle;

[0029] Figure 6 It is the Figure 4 Enlarged view of position B in the middle.

[0030] Mark for explaining:

[0031] 1, cryopreservation device body;2, cover plate;3, controller;4, cell rack;5, vertical rod;6, limiting structure;61, limiting groove;62, limiting block;63, positioning groove;64, positioning block;65, handle;66, fixed block;7, adjusting structure;71, isolation plate;72, adjusting plate;73, vertical block;74, horizontal rod;75, horizontal block;8, moving groove;9, horizontal groove;10, sealing groove;11, sealing strip;12, round groove;13, damping rod;14, torsional spring;15, observation window;16, anti-skid block. Specific implementation

[0032] Exemplary embodiments of the present application will be described herein below with reference to the accompanying drawings. While exemplary embodiments of the present application are shown in the drawings, it is understood that the present application can be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that this application will be thoroughly and completely understood as a complete disclosure of the present application and will fully convey the scope of the application to those skilled in the art.

[0033] As Figures 1 to 6 shown, the utility model provides a kind of stem cell exosome active cell cryopreservation device, including cryopreservation device body 1, the top of cryopreservation device body 1 is provided with cover plate 2, the right side of the front of cryopreservation device body 1 top is equipped with controller 3, the inside of cryopreservation device body 1 is provided with cell rack 4, the bottom of the inner wall of cryopreservation device body 1 is fixedly connected with vertical rod 5 in the center, cell rack 4 is located on the surface of vertical rod 5, the center of cell rack 4 top is provided with limiting structure 6, limiting structure 6 includes limiting groove 61, limiting groove 61 is located on the top of vertical rod 5 surface, limiting groove 61 is provided with limiting block 62 in the inside, limiting block 62 is movably connected with the top of cell rack 4 by round rod on the bottom, limiting block 62 left side is equipped with positioning groove 63, positioning groove 63 is slidably connected with positioning block 64 in the inside, positioning block 64 right side is fixedly connected with handle 65, positioning block 64 surface is slidably connected with fixed block 66, the bottom of fixed block 66 is fixedly connected with the top of cell rack 4, the inside of cell rack 4 is provided with adjusting structure 7.

[0034] As Figures 1 to 6As shown, the adjusting structure 7 comprises an isolation plate 71, the surface of the isolation plate 71 is slidably connected with an adjusting plate 72, the top of the adjusting plate 72 is fixedly connected with a vertical block 73, the front surface of the vertical block 73 is fixedly connected with a horizontal rod 74, and the surface of the horizontal rod 74 is sleeved with a horizontal block 75.

[0035] As shown in the figure, Figures 1 to 6 The top of the adjusting plate 72 is provided with a moving groove 8, the vertical block 73 is located in the moving groove 8, and the left side of the adjusting plate 72 is provided with a horizontal groove 9, and the horizontal block 75 is located in the horizontal groove 9.

[0036] As shown in the figure, Figures 1 to 6 The bottom of the freezing device body 1 is provided with a sealing groove 10, and the sealing groove 10 is internally provided with a sealing strip 11.

[0037] As shown in the figure, Figures 1 to 6 The bottom of the freezing device body 1 is provided with a sealing groove 10, and the sealing groove 10 is internally provided with a sealing strip 11.

[0038] As shown in the figure, Figures 1 to 6 The surface of the horizontal rod 74 is fixedly connected with a torsion spring 14, and the other end of the torsion spring 14 is fixedly connected with the outer side of the horizontal block 75.

[0039] As shown in the figure, Figures 1 to 6 Both sides of the freezing device body 1 are provided with an observation window 15, and the four peripheries of the bottom of the freezing device body 1 are fixedly connected with anti-skid blocks 16.

[0040] In the embodiment of the utility model, first, the cover plate 2 is opened, then the cell box to be frozen is properly placed in the cell rack 4 matched with its size, so as to effectively utilize the space, subsequently, the horizontal block 75 is rotated clockwise on the surface of the horizontal rod 74 to cancel the limiting of the adjusting plate 72, and the adjusting plate 72 is moved to the appropriate position according to the size of the cell box, then, the installation of the cell rack 4 is carried out: the handle 65 is pulled outward to make the positioning block 64 disengage from the positioning groove 63, the limiting block 62 is rotated clockwise so as to stably place the cell rack 4 into the freezing device body 1 through the vertical rod 5, the positioning block 64 is positioned in cooperation with the positioning groove 63 to position the limiting block 62, and then the limiting block 62 is counterclockwise rotated to limit the vertical rod 5, so as to ensure the stability of the cell rack 4, then, the controller 3 is operated to release liquid nitrogen into the cell rack 4, the temperature of the cell box is rapidly reduced to realize freezing, the release amount of liquid nitrogen and the temperature change are closely monitored through the observation window 15, and the key parameters are recorded, when the cell reaches the required freezing state, the controller 3 is closed, the cell box is carefully taken out, and the cell rack 4 is cleaned for next use.

[0041] The above is the preferred embodiment of the present application, it should be pointed out that, for those skilled in the ordinary in the art, without departing from the principles described in the present application, can be made several improvements and refinements, these improvements and refinements should also be considered as the scope of protection of the present application.

Claims

1. An active cell cryogenic device for stem cell exosomes, comprising a cryogenic device body (1), the top of the cryogenic device body (1) is provided with a cover plate (2), the right side of the front of the top of the cryogenic device body (1) is installed with a controller (3), the inside of the cryogenic device body (1) is provided with a cell rack (4), characterized in that, The bottom of the inner wall of the freezing device body (1) is fixedly connected with a vertical rod (5), the cell rack (4) is located on the surface of the vertical rod (5), the top of the cell rack (4) is provided with a limiting structure (6), the limiting structure (6) comprises a limiting groove (61), the limiting groove (61) is located on the top of the surface of the vertical rod (5), the inside of the limiting groove (61) is provided with a limiting block (62), the bottom of the limiting block (62) is movably connected with the top of the cell rack (4) through a round rod, a positioning groove (63) is formed in the left side of the limiting block (62), a positioning block (64) is slidably connected in the positioning groove (63), a handle (65) is fixedly connected to the right side of the positioning block (64), a fixed block (66) is slidably connected to the surface of the positioning block (64), the bottom of the fixed block (66) is fixedly connected with the top of the cell rack (4), and the inside of the cell rack (4) is provided with an adjusting structure (7).

2. The active cell cryo-preservation device of stem cell exosomes according to claim 1, wherein, The adjusting structure (7) comprises an isolation plate (71), the surface of the isolation plate (71) is slidably connected with an adjusting plate (72), the top of the adjusting plate (72) is fixedly connected with a vertical block (73), the front surface of the vertical block (73) is fixedly connected with a horizontal rod (74), and the surface of the horizontal rod (74) is sleeved with a horizontal block (75).

3. The active cell cryo-preservation device of stem cell exosomes according to claim 2, wherein, The top of the adjusting plate (72) is provided with a moving groove (8), the vertical block (73) is located in the moving groove (8), and a horizontal groove (9) is formed in the left side of the adjusting plate (72).

4. The active cell cryo-preservation device of stem cell exosomes according to claim 1, wherein, The bottom of the freezing device body (1) is provided with a sealing groove (10), and the sealing groove (10) is internally provided with a sealing strip (11).

5. The active cell cryo-preservation device of stem cell exosomes according to claim 1, wherein, The bottom of the freezing device body (1) is provided with a sealing groove (10), and the sealing groove (10) is internally provided with a sealing strip (11).

6. The active cell cryo-preservation device of stem cell exosomes according to claim 2, wherein, The surface of the horizontal rod (74) is fixedly connected with a torsional spring (14), and the other end of the torsional spring (14) is fixedly connected with the outer side of the horizontal block (75).

7. The active cell cryo-preservation device of stem cell exosomes according to claim 1, wherein, The two sides of the freezing device body (1) are provided with observation windows (15), and the four around the bottom of the freezing device body (1) are fixedly connected with anti-skid blocks (16).

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