Mouse head fixing device for mouse brain separation

Abstract

The utility model discloses a mouse head fixing device for mouse brain separation, and relates to the technical field of biological research. Comprising an operation plate, a liquid groove is formed in the operation plate, two pairs of supporting legs are arranged on the lower wall face of the operation plate, a U-shaped cavity and an open groove are formed in the operation plate, two-way lead screws are rotationally connected into the two side wall faces of the U-shaped cavity, a sliding rod is connected between the two side wall faces of the U-shaped cavity, and a rotary knob is rotationally connected into the operation plate. The rotary knob is connected with the two-way lead screw, the two-way lead screw is in threaded connection with a pair of moving plates, and the pair of moving plates are both installed on the sliding rod in a sliding mode. According to the auxiliary craniotomy device, a structure for assisting craniotomy is designed, and the auxiliary craniotomy device can be provided with a structure for clamping a fur layer, so that the influence of the fur layer on craniotomy is avoided, the operation of manually fixing the fur layer is reduced, an operator can finish craniotomy more intensively, and the integrity of rat brain separation is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological research technical field, concretely is a fixed mouse head device for mouse brain separation. BACKGROUND

[0002] Brain is the main part of central nervous system, is located in the cranial cavity. It is the organ of regulating body function, also is the material basis of consciousness, spirit, language, learning, memory and intelligence and other high-level neural activities, researchers when researching Alzheimer's disease (AD) or Parkinson's disease (Parkinson's disease, PD) these nervous system degenerative diseases, cannot avoid needing to study brain, SD rat is the mouse species that animal experiment is widely used, so the general nervous system degenerative disease animal model is molded with SD rat, but when the rat brain is taken, its brain skeleton is hard, and when the brain is separated, the brain integrity needs to be guaranteed, so it is not easy to take.

[0003] The existing patent (announcement number: CN211962267U) discloses a device for fixing mouse head when separating rat brain, which comprises a base, a head placing groove, a head fixing clamp, a head fixer and a cleaning groove, a head placing groove is formed in the middle of one side of the upper surface of the base, the bottom surface of the head placing groove is inclined, a drawer type shallow groove is also formed on the base corresponding to one side of the head placing groove, the groove bottom of the shallow groove is lower than the lowest point of the groove bottom of the head placing groove, a mesh wire net is arranged on the groove opening of the shallow groove, a plurality of blood overflow holes are uniformly and interval formed on the side wall of the head placing groove close to the shallow groove, head fixers are symmetrically arranged on the side walls of the base corresponding to the position of the head placing groove, and a head fixing clamp and a cleaning groove are also fixed on the base corresponding to the position of the head placing groove.

[0004] The inventor found that the prior art has the following problems in the process of implementing the utility model: the existing device lacks a structure capable of fixing the fur layer, the volume of the mouse brain is small, and the fur layer of the head will rebound and cover the skull position without control, and it is not convenient to manually press the fur layer, but the existing device lacks a structure capable of fixing the fur layer. UTILITY MODEL CONTENTS

[0005] In view of the defects of the prior art, the utility model provides a device for fixing mouse head for mouse brain separation, which solves the problem that the existing device lacks a structure capable of fixing the fur layer, the volume of the mouse brain is small, and the fur layer of the head will rebound and cover the skull position without control, and it is not convenient to manually press the fur layer, but the existing device lacks a structure capable of fixing the fur layer.

[0006] To achieve the above object, the utility model discloses a fixed mouse head device for mouse brain separation, including operation board, the operation board is opened liquid groove in, the lower wall of operation board is equipped with two pairs of support leg, the operation board is opened U type cavity and slot, both sides wall of U type cavity are rotatably connected with two -way screw rod, the both sides wall between U type cavity is connected with slide rod, rotatably connected with screw knob in the operation board, the screw knob is connected with two -way screw rod, a pair of mobile plate is connected on two -way screw rod, a pair of mobile plate all slide installation on slide rod, a pair of insertion hole is opened between U type cavity and slot, the side wall of a pair of mobile plate all is connected with round bar, a pair of round bar is inserted in a pair of insertion hole respectively, a pair of round bar one end all is connected with clamping plate, a pair of round bar all is connected with U type board, a pair of U type board all is connected with clamp.

[0007] Preferably, the pair of clamps each has an inclination angle.

[0008] Preferably, the liquid groove contains PBS buffer solution.

[0009] Preferably, the slot is provided with a baffle at one side of the lower end, and the baffle is arc-shaped.

[0010] Beneficial effects

[0011] The utility model provides a fixed mouse head device for mouse brain separation, the utility model discloses the structure of auxiliary craniotomy, first mouse head is inserted into the slot, mouse eye is covered by baffle, manually rotates screw knob and drives a pair of clamping plate to move and holds the left and right sides of mouse brain fixed, and the left and right sides of mouse brain fur layer are clamped in a pair of clamps respectively, and the fur layer is separated from both sides by a pair of clamps, and the mouse brain is dug out and placed in the liquid groove with long -handled medicine spoon, and is soaked in PBS buffer solution. The device can clamp the fur layer, avoid the influence of the fur layer on craniotomy, reduce the operation of manual fur layer fixation, make the operator more focused to complete craniotomy, and improve the completeness of mouse brain separation. BRIEF DESCRIPTION OF DRAWINGS

[0012] Fig. 1 It is the whole structure schematic diagram of the utility model.

[0013] Fig. 2 It is the local structure schematic diagram of the utility model.

[0014] In the drawing: 1, operation board, 2, liquid groove, 3, slot, 4, U type cavity, 5, two -way screw rod, 6, slide rod, 7, mobile plate, 8, round bar, 9, insertion hole, 10, baffle, 11, U type board, 12, clamping plate, 13, clamp, 14, screw knob. DETAILED DESCRIPTION

[0015] The technical solutions in the embodiments of the present application will be clearly and completely described with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0016] Please refer to Figs. 1-2 The present application provides a technical solution: a device for fixing the head of a mouse for brain separation, comprising an operation plate 1, a liquid tank 2 is formed in the operation plate 1, two pairs of supporting legs are arranged on the lower wall surface of the operation plate 1, a U-shaped cavity 4 and a slot 3 are formed in the operation plate 1, a pair of bidirectional lead screws 5 are rotatably connected to the two side wall surfaces of the U-shaped cavity 4, a sliding rod 6 is connected between the two side wall surfaces of the U-shaped cavity 4, a screw knob 14 is rotatably connected to the operation plate 1, the screw knob 14 is connected to the bidirectional lead screws 5, a pair of moving plates 7 are threadedly connected to the bidirectional lead screws 5, the moving plates 7 are slidably installed on the sliding rod 6, a pair of insertion holes 9 are formed between the U-shaped cavity 4 and the slot 3, a pair of side wall surfaces of the moving plates 7 are connected to a pair of round rods 8, the round rods 8 are movably inserted into the insertion holes 9, a pair of clamping plates 12 are connected to one end of the round rods 8, a pair of U-shaped plates 11 are connected to the round rods 8, and a pair of clamps 13 are connected between the U-shaped plates 11.

[0017] The present embodiment is further provided with a pair of clamps 13, which have an inclined angle.

[0018] The present embodiment is further provided with a pair of clamps 13, which have an inclined angle.

[0019] The present embodiment is further provided with a pair of clamps 13, which have an inclined angle.

[0020] The detailed connection means is a known technology in the art, and the working principle and process will be mainly introduced below, and the specific work is as follows.

[0021] Example: First, place the euthanized mouse with severed neck under the operating board 1. Insert the mouse's head into the slot 3 with one hand, covering the mouse's eyes with the baffle 10. Manually rotate the knob 14 to rotate the bidirectional lead screw 5. The rotation of the bidirectional lead screw 5 causes a pair of moving plates 7 to move relative to each other along the slide bar 6. The movement of the pair of moving plates 7 causes a pair of round rods 8 to move. The movement of the pair of round rods 8 causes a pair of clamping plates 12 to move. The movement of the pair of clamping plates 12 clamps and fixes the mouse's brain from the left and right sides. Spray the back of the mouse's brain with PBS to prevent the fur from sticking. Cut the mouse's neck to the nose with surgical scissors. Clamp the fur layer on both sides of the mouse's brain into a pair of clamps 13. Gently slide the scalpel along the midline of the mouse's skull with a scalpel blade. Manually rotate the knob 14 in the opposite direction to separate the pair of clamping plates 12 and release the clamps. The pair of clamps 13 separate the fur layer from both sides. Separate the skull with surgical scissors. Use a long-handled medicine spoon to remove the mouse's brain and place it in the liquid tank 2 to soak in PBS buffer.

[0022] It should be noted that in this paper, relational terms such as first and second are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations.

Claims

1. A device for fixing a mouse head for brain dissection, comprising an operation plate (1), characterized in that, The operation plate (1) is provided with a liquid tank (2), the lower wall of the operation plate (1) is provided with two pairs of supporting legs, the operation plate (1) is provided with a U-shaped cavity (4) and a slot (3), the two side walls of the U-shaped cavity (4) are rotatably connected with a bidirectional screw rod (5), the two side walls of the U-shaped cavity (4) are connected with a sliding rod (6), the operation plate (1) is rotatably connected with a rotating knob (14), the rotating knob (14) is connected with the bidirectional screw rod (5), the bidirectional screw rod (5) is threadedly connected with a pair of moving plates (7), the pair of moving plates (7) are slidably installed on the sliding rod (6), a pair of insertion holes (9) are formed between the U-shaped cavity (4) and the slot (3), the side walls of the pair of moving plates (7) are connected with a pair of round rods (8), the pair of round rods (8) are movably inserted into the pair of insertion holes (9) respectively, the ends of the pair of round rods (8) are connected with clamping plates (12), the pair of round rods (8) are connected with U-shaped plates (11), and the U-shaped plates (11) are connected with clamps (13).

2. The apparatus for fixing a mouse head for mouse brain dissociation according to claim 1, wherein The pair of clamps (13) have an inclined angle.

3. The apparatus for fixing a mouse head for mouse brain dissociation according to claim 1, wherein The liquid tank (2) is placed with PBS buffer solution.

4. The apparatus for fixing a mouse head for brain dissection according to claim 1, wherein The slot (3) is provided with a baffle (10) at the lower end of one side, and the baffle (10) is arc-shaped.

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