Film cutting device applied to Western blot experiment

Abstract

The utility model provides a film cutting device applied to a Western blot experiment, and belongs to the technical field of film cutting experiments. Comprising a box body; the box cover covers the upper end of the box body; the film cutting platform is arranged in the box body, and a plurality of scale marks are engraved on the film cutting platform; the two lifting plates are arranged at the left end and the right end of the film cutting platform respectively; the lifting driving assembly is arranged in the box body and used for adjusting the use height of the film cutting platform; the sliding frame is arranged at the upper part in the box body; the film cutter is arranged at the lower end of the sliding frame; the two adjusting shafts are fixed to the left end and the right end of the film cutting platform, and the lifting plates are rotationally connected with the adjacent adjusting shafts; the film cutting height of the film cutting platform can be conveniently adjusted, the film cutting angle of the film cutter can be conveniently adjusted, operation is easy and convenient, efficiency is high, a TBST solution is stored in the box body, incubation and cleaning of a film before and after film cutting are facilitated, the film can be kept wet during film cutting, it is ensured that the experiment result is not affected, and the accuracy of the experiment result is guaranteed.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of film cutting experiments, and particularly relates to a film cutting device applied to Western blot experiments. BACKGROUND

[0002] Western Blot, also known as protein blotting, is a commonly used experimental method based on specific immune reactions between antibodies and antigens in molecular biology, biochemistry and immunogenetics. It is mainly used for detecting the expression amount of specific proteins, separating proteins by electrophoresis, then transferring the proteins to a solid support (such as a PVDF membrane), and then detecting using specific antibodies. Western blot is a commonly used protein analysis technology, and film cutting is one of the key steps.

[0003] At present, the traditional film cutting method uses scissors or blades for manual operation. Since manual film cutting needs to repeatedly adjust the position and angle of the film, the operation is cumbersome and inefficient, and manual operation cannot guarantee the cutting precision, which may cause the film pieces to be of different sizes, affecting the experimental results. Moreover, manual operation with sharp scissors or blades has safety hazards such as finger cuts, and the film exposed to the air is prone to dryness, which may adversely affect the experimental results.

[0004] In view of the above problems, the application designs a film cutting device applied to Western blot experiments to solve the technical problems of cumbersome film cutting operation, low efficiency, poor precision, low repeatability, and safety hazards. CONTENT OF THE INVENTION

[0005] The application aims to at least solve one of the technical problems existing in the prior art or related art.

[0006] To this end, the application designs a film cutting device applied to Western blot experiments to solve the technical problems of cumbersome film cutting operation, low efficiency, poor precision, low repeatability, and safety hazards.

[0007] The film cutting device applied to Western blot experiments provided by the application comprises a box body, a box cover on the upper end of the box body, a film cutting platform in the box body, a plurality of scale lines engraved on the film cutting platform, two lifting plates respectively on the left and right ends of the film cutting platform, a lifting drive assembly in the box body for adjusting the use height of the film cutting platform, a sliding frame in the upper part of the box body, a film cutting knife on the lower end of the sliding frame, two adjusting shafts fixed on the left and right ends of the film cutting platform, the lifting plates are rotationally connected with the adjacent adjusting shafts, a film cutting position adjusting assembly above the film cutting knife for adjusting the film cutting position of the film cutting knife, and an angle adjusting assembly on the right side of the film cutting platform for adjusting the inclination angle of the film cutting platform to facilitate observation of the film cutting process and film transfer.

[0008] In some possible embodiments, the lifting drive assembly includes: a guide rod fixed to the left side of the housing, wherein a lifting plate is slidably sleeved with the guide rod; a first lead screw rotatably connected to the right side of the housing, wherein another lifting plate is threadedly sleeved with the first lead screw; a secondary bevel gear fixedly sleeved around the periphery of the first lead screw; a main bevel gear meshing with the secondary bevel gear; and a rotating shaft fixed to the right end of the main bevel gear, wherein the rotating shaft rotatably extends through the right side of the housing, and a rocker arm is fixed to the right end of the rotating shaft.

[0009] In some possible embodiments, the film cutting position adjustment assembly includes: a slide rod, fixed to the upper end of the housing, with the slide frame slidably sleeved with the slide rod; and a first threaded locking rod, threadedly connected to the upper end of the slide frame, with the lower end of the first threaded locking rod contacting the slide rod.

[0010] In some possible embodiments, the film cutting position adjustment assembly further includes: a sliding block, wherein the lower end of the sliding frame has a sliding groove, and the sliding block is slidably connected to the sliding groove; a second threaded locking rod, threadedly connected to the outside of the sliding frame, and the second threaded locking rod extends into the sliding groove; a damping shaft, rotatably connected to the lower end of the sliding block; a mounting block, disposed below the damping shaft, wherein the film cutting blade is fixed to the lower end of the mounting block; and a threaded connecting rod, fixed to the upper end of the mounting block, wherein the lower end of the damping shaft has a threaded connecting groove, and the threaded connecting rod is threadedly connected to the threaded connecting groove.

[0011] In some possible embodiments, the angle adjustment assembly includes: a concave guide plate fixed to the upper end of an adjacent lifting plate; a toothed plate slidably connected inside the concave guide plate; a synchronous gear fixedly sleeved around an adjacent adjusting shaft, the toothed plate meshing with the synchronous gear; and a second lead screw rotating from front to back through the concave guide plate, the toothed plate being threadedly sleeved with the second lead screw.

[0012] In some possible embodiments, pressure rods are provided on both sides of the upper end of the film cutting platform.

[0013] In some possible embodiments, a transparent observation plate is embedded in the front of the box, and the transparent observation plate is made of transparent acrylic material.

[0014] In some possible embodiments, a drain valve is connected to the lower right end of the housing.

[0015] In some possible embodiments, the lower end of the box is connected to an anti-slip pad.

[0016] Compared with the prior art, the technical solution provided in this application has at least the following beneficial effects:

[0017] This application provides a membrane cutting device for Western blot experiments. The cutting platform is manually raised and lowered for easy adjustment of the cutting height. The sliding frame allows for left-right adjustment of the cutting blade's position, and the sliding block controls the blade's forward and backward movement. The damping shaft allows for adjustment of the cutting angle. The device is simple to operate and highly efficient. Since the cutting blade is fixed to the lower end of the mounting block, it avoids the safety hazards associated with manual operation. The graduated lines on the cutting platform and the movable pressure rod allow for precise membrane positioning, ensuring cutting accuracy and repeatability. The presence of TBST solution within the container facilitates membrane incubation and cleaning before and after cutting, keeping the membrane moist during cutting and ensuring that the experimental results are accurate and unaffected.

[0018] Additional aspects and advantages of this application will become apparent in the following description or may be learned by practice of this application. Attached Figure Description

[0019] The above and / or additional aspects and advantages of this application will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0020] Figure 1 This is a schematic diagram of the overall internal structure of a membrane cutting device used in Western blot experiments according to some embodiments of this application;

[0021] Figure 2 This is a partially enlarged structural diagram of a membrane cutting device used in Western blot experiments according to some embodiments of this application;

[0022] Figure 3 This is a partial enlarged structural diagram of a membrane cutting device used in Western blot experiments according to some embodiments of this application;

[0023] Figure 4 This is a top view of the membrane cutting platform of a membrane cutting device applied to a Western blot experiment according to some embodiments of this application;

[0024] Figure 5 This is a schematic diagram of the internal structure of the sliding frame of a membrane cutting device applied to a Western blot experiment according to some embodiments of this application;

[0025] Figure 6 This is a schematic diagram of the internal structure of the damping shaft of a film cutting device applied to a Western blot experiment according to some embodiments of this application;

[0026] Figure 7This is a side view of the lifting plate structure of a film cutting device applied to a Western blot experiment according to some embodiments of this application;

[0027] Figure 8 This is a schematic diagram of the overall external structure of a membrane cutting device used in Western blot experiments according to some embodiments of this application.

[0028] Figure label:

[0029] 1. Box body; 2. Box cover; 3. Film cutting platform; 4. Lifting plate; 5. Infusion tube; 6. Main bevel gear; 7. Secondary bevel gear; 8. Rotating shaft; 9. Rocker arm; 10. Film pressing rod; 11. Guide rod; 12. Slide rod; 13. Sliding frame; 14. First threaded locking rod; 15. Slide groove; 16. Slider; 17. Second threaded locking rod; 18. Damping rotating shaft; 19. Mounting block; 20. Film cutting knife; 21. Threaded connecting rod; 22. Threaded connecting groove; 23. First lead screw; 24. Concave guide plate; 25. Convex tooth plate; 26. Adjusting shaft; 27. Synchronous gear; 28. Second lead screw; 29. ​​Transparent observation plate; 30. Drain valve; 31. Anti-slip pad. Detailed Implementation

[0030] To better understand the above-mentioned objectives, features, and advantages of this application, the application will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0031] Many specific details are set forth in the following description in order to provide a full understanding of this application. However, this application may also be implemented in other ways different from those described herein. Therefore, the scope of protection of this application is not limited to the specific embodiments disclosed below.

[0032] The following reference Figures 1 to 8 This application describes a membrane cutting device for use in Western blot experiments, according to some embodiments thereof.

[0033] Please see Figures 1 to 8 The present application provides a membrane cutting device for Western blot experiments, comprising a box body 1; a box cover 2 covering the upper end of the box body 1; and a membrane cutting platform 3 disposed inside the box body 1. The membrane cutting platform 3 is engraved with several scale lines, and pressure rods 10 are provided on both sides of the upper end of the membrane cutting platform 3.

[0034] In this embodiment, by opening the box cover 2 and with TBST solution inside the box body 1, the membrane to be cut is placed on the membrane cutting platform 3, and then the membrane is pressed down by the membrane pressing rod 10. The membrane can be accurately positioned by combining the scale lines on the membrane cutting platform 3 and the movable membrane pressing rod 10, preventing membrane position displacement and ensuring membrane cutting accuracy and repeatability. Moreover, the TBST solution is in contact with the membrane, which facilitates membrane incubation and cleaning before and after cutting, helps to keep the membrane moist during cutting, ensures that it will not affect the experimental results, and guarantees the accuracy of the experimental results.

[0035] In some embodiments, two lifting plates 4 are respectively disposed at the left and right ends of the film cutting platform 3; a lifting drive assembly is disposed inside the box 1 and is used to adjust the working height of the film cutting platform; the lifting drive assembly includes: a guide rod 11, fixed inside the left side of the box 1, wherein one lifting plate 4 is slidably sleeved with the guide rod 11; a first lead screw 23, rotatably connected inside the right side of the box 1, wherein the other lifting plate 4 is threadedly sleeved with the first lead screw 23; a secondary bevel gear 7, fixedly sleeved around the first lead screw 23; a main bevel gear 6, meshing with the secondary bevel gear 7; a rotating shaft 8, fixed to the right end of the main bevel gear 6, and the rotating shaft 8 rotatably passes through the right side of the box 1, wherein a rocker arm 9 is fixed to the right end of the rotating shaft 8.

[0036] In this embodiment, after the film is placed on the film cutting platform 3, the rotating shaft 8 and the main bevel gear 6 are rotated by the hand crank 9. The main bevel gear 6 will drive the secondary bevel gear 7 and the first lead screw 23 to rotate. When the first lead screw 23 rotates, it will drive the film cutting platform 3 to move up and down along the guide rod 11, thereby controlling the lifting and lowering of the film cutting platform 3 and making it convenient to adjust the film cutting height of the film cutting platform 3 until the film cutting knife 20 can cut the film.

[0037] In some embodiments, a sliding frame 13 is disposed in the upper part of the box body 1; a film cutting blade 20 is disposed at the lower end of the sliding frame 13; and a film cutting position adjustment assembly is disposed above the film cutting blade 20 for adjusting the alignment of the film cutting blade 20 with the film cutting position. The film cutting position adjustment assembly includes: a sliding rod 12, fixed in the upper part of the box body 1, with the sliding frame 13 slidably sleeved with the sliding rod 12; and a first threaded locking rod 14, threadedly connected to the upper end of the sliding frame 13, with the lower end of the first threaded locking rod 14 contacting the sliding rod 12.

[0038] In this embodiment, by twisting the first threaded locking rod 14 upwards and ensuring that the first threaded locking rod 14 is no longer pressed against the slide rod 12, the sliding frame 13 is pulled to move left and right along the slide rod 12, which facilitates the adjustment of the left and right cutting position of the film cutting knife 20. Moreover, by tightening the first threaded locking rod 14 downwards, the first threaded locking rod 14 is pressed against the slide rod 12, preventing the sliding frame 13 from causing the film cutting knife 20 to slide accidentally, thus ensuring the stability of the film cutting knife 20 after the position is adjusted.

[0039] In some embodiments, the film cutting position adjustment assembly further includes: a sliding block 16, a sliding groove 15 is provided at the lower end of the sliding frame 13, and the sliding block 16 is slidably connected to the sliding groove 15; a second threaded locking rod 17, which is threadedly connected to the outside of the sliding frame 13 and extends into the sliding groove 15; and a damping rotating shaft 18, which is rotatably connected to the lower end of the sliding block 16.

[0040] In this embodiment, the sliding block moves back and forth within the groove 15, facilitating the movement of the cutting blade 20 for cutting. When the sliding block does not need to move, tightening the second threaded locking rod 17 presses the sliding block, preventing it from continuing to slide along the groove 15. Furthermore, the rotation of the damping shaft 18 drives the cutting blade 20 to rotate, allowing for easy adjustment of the cutting angle. In summary, this makes the cutting operation simple and efficient.

[0041] In some embodiments, the mounting block 19 is located below the damping shaft 18, and the cutting blade 20 is fixed to the lower end of the mounting block 19; the threaded connecting rod 21 is fixed to the upper end of the mounting block 19, and the lower end of the damping shaft 18 has a threaded connecting groove 22, and the threaded connecting rod 21 is threadedly connected to the threaded connecting groove 22.

[0042] In this embodiment, by unscrewing the threaded connecting rod 21 out of the threaded connecting groove 22, the film cutting knife 20 can be easily disassembled, thereby allowing the user to replace the film cutting knife 20 with different specifications according to different experimental needs.

[0043] In some embodiments, two adjusting shafts 26 are fixed to the left and right ends of the film cutting platform 3, and the lifting plates 4 are rotatably connected to the adjacent adjusting shafts 26. An angle adjustment assembly is located on the right side of the film cutting platform 3 and is used to adjust the tilt angle of the film cutting platform 3 to facilitate observation of the film cutting process and film transfer. The angle adjustment assembly includes: a concave guide plate 24, fixed to the upper end of the adjacent lifting plate 4; a toothed plate 25, slidably connected to the concave guide plate 24; a gear 27, fixedly sleeved around the periphery of the adjacent adjusting shaft 26, and the toothed plate 25 meshes with the gear 27; and a second lead screw 28, which rotates from front to back and passes through the concave guide plate 24, and the toothed plate 25 is threadedly sleeved with the second lead screw 28.

[0044] In this embodiment, by turning the second lead screw 28, the toothed plate 25 is driven to slide back and forth along the concave plate. The toothed plate 25 will drive the gear and the adjusting shaft 26 to rotate. Therefore, the rotating shaft 8 will drive the film cutting platform 3 to tilt, which is beneficial to tilt the film cutting platform 3 at a certain angle, making it convenient to observe the film cutting process and the film transfer.

[0045] In some embodiments, a transparent observation plate 29 is embedded in the front of the box body 1, and the transparent observation plate 29 is made of transparent acrylic material.

[0046] In this embodiment, by providing a transparent observation plate 29 on the box 1, it is convenient to observe the liquid level of the TBST solution and the incubation status of the membrane inside the box 1.

[0047] In some embodiments, a drain valve 30 is connected to the lower right end of the housing 1.

[0048] In this embodiment, by setting a drain valve 30, the drain valve 30 is opened after the experiment to facilitate the discharge of waste liquid in the box 1.

[0049] In some embodiments, an anti-slip pad 31 is attached to the lower end of the box body 1.

[0050] In this embodiment, by providing an anti-slip pad 31 under the box body 1, the friction between the box body 1 and the supporting surface is increased, which helps to prevent the box body 1 from sliding during the experiment and improves the safety of the experiment.

[0051] In this application, it should be noted that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0052] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0053] In this application, unless otherwise expressly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. The term "multiple" refers to two or more, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0054] In this application, unless otherwise expressly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0055] In this application, the terms "one embodiment," "some embodiments," "specific embodiment," etc., refer to a specific feature, structure, material, or characteristic described in connection with that embodiment or example, which is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0056] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A membrane cutting device for use in Western blot experiments, characterized in that, It includes: Box body (1); Box cover (2), cover on the upper end of box body (1); Film cutting platform (3) is provided in the box body (1), the film cutting platform (3) is engraved with several scale lines; Two lifting plates (4) are respectively arranged at the left and right ends of the film cutting platform (3); Lifting drive assembly is arranged in the box body (1), which is used for adjusting the use height of the film cutting platform; Sliding frame (13) is arranged in the upper part of the box body (1); Film cutting knife (20) is arranged at the lower end of the sliding frame (13); Two adjusting shafts (26) are fixed at the left and right ends of the film cutting platform (3), and the lifting plates (4) are rotatably connected with the adjacent adjusting shafts (26); Film cutting position adjusting assembly is arranged above the film cutting knife (20), which is used for adjusting the film cutting position of the film cutting knife (20); Angle adjusting assembly is arranged on the right side of the film cutting platform (3), which is used for adjusting the inclination angle of the film cutting platform (3) to facilitate observation of the film cutting process and the transfer of the film.

2. The membrane cutter for use in Western blot experiments according to claim 1, characterized in that The lifting drive assembly includes: A guide rod (11) is fixed to the left side of the box body (1), one of the lifting plates (4) is slidably connected with the guide rod (11); A first screw rod (23) is rotatably connected to the right side of the box body (1), the other lifting plate (4) is threadedly connected with the first screw rod (23); A sub bevel gear (7) is fixedly connected to the periphery of the first screw rod (23); A main bevel gear (6) is engaged with the sub bevel gear (7); A rotating shaft (8) is fixed to the right end of the main bevel gear (6), and the rotating shaft (8) penetrates through the right side of the box body (1), and a rocker (9) is fixed to the right end of the rotating shaft (8).

3. The membrane cutter of claim 1, wherein the membrane cutter is applied to a Western blot experiment. The film cutting position adjusting assembly includes: A sliding rod (12) is fixed to the upper end of the box body (1), and the sliding frame (13) is slidably connected with the sliding rod (12); A first threaded locking rod (14) is threadedly connected to the upper end of the sliding frame (13), and the lower end of the first threaded locking rod (14) is in contact with the sliding rod (12).

4. The membrane cutter of claim 3, wherein the membrane cutter is configured to cut a membrane into a plurality of strips, each strip having a width of 1 mm or less. The film cutting position adjusting assembly further includes: A sliding block (16) is slidably connected with a sliding groove (15) formed in the lower end of the sliding frame (13); A second threaded locking rod (17) is threadedly connected to the outer side of the sliding frame (13), and the second threaded locking rod (17) extends into the sliding groove (15); A damping rotating shaft (18) is rotatably connected to the lower end of the sliding block (16); An installation block (19) is arranged below the damping rotating shaft (18), and the film cutting knife (20) is fixed to the lower end of the installation block (19); A threaded connecting rod (21) is fixed to the upper end of the installation block (19), a threaded connecting groove (22) is formed in the lower end of the damping rotating shaft (18), and the threaded connecting rod (21) is threadedly connected with the threaded connecting groove (22).

5. The membrane cutter of claim 1, wherein the membrane cutter is applied to a Western blot experiment. The angle adjusting assembly includes: A concave guide plate (24) is fixed to the upper end of the adjacent lifting plate (4); A convex tooth plate (25) is slidably connected in the concave guide plate (24); A synchronous gear (27) is fixedly connected to the periphery of the adjacent adjusting shaft (26), and the convex tooth plate (25) is engaged with the synchronous gear (27). Second screw rod (28) rotates from front to back through the concave guide plate (24), and the convex tooth plate (25) is threadedly connected with the second screw rod (28).

6. The membrane cutter of claim 1, wherein, The film pressing rods (10) are arranged on both sides of the upper end of the film cutting platform (3).

7. The membrane cutter of claim 1, wherein the membrane cutter is applied to a Western blot experiment. The front face of the box body (1) is inlaid with a transparent observation plate (29) made of transparent acrylic material.

8. The membrane cutter of claim 1, wherein, The box body (1) is communicated with a liquid discharge valve (30) at the lower right end.

9. The membrane cutter of claim 1, wherein, The box body (1) is connected with an anti-skid pad (31) at the lower end.

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