Centrifugal device for stem cell exosome processing

Abstract

The utility model discloses a centrifugal device for stem cell exosome processing, which comprises a base, the outer wall of the top of the base is fixedly connected with a side plate, the outer wall of the side plate is provided with a supporting plate, the supporting plate is rotatably connected with a processing box, a first centrifugal mechanism is arranged between the processing box and the side plate, and a second centrifugal mechanism is arranged between the processing box and the side plate. A second centrifugal mechanism is arranged on the outer wall of the treatment box, and the top of the treatment box is in threaded connection with a top cover. Raw materials in the treatment box can be stirred, crushed and scraped through the crushing mechanism arranged in the treatment box, and can be aerated through the aeration mechanism arranged in the treatment box, so that the crushing and centrifuging effects are further improved; the second centrifugal mechanism arranged on the outer wall of the treatment box can enable the treatment box to have the horizontal centrifugal capacity, the first centrifugal mechanism can enable the treatment box to have the vertical centrifugal capacity, and it is guaranteed that raw materials in the treatment box can be efficiently centrifuged.

Description

Technical Field

[0001] This utility model relates to the field of centrifugation device technology, and in particular to a centrifugation device for processing stem cell exosomes. Background Technology

[0002] Exosomes are small membrane vesicles containing complex RNA and proteins. Currently, they specifically refer to disc-shaped vesicles with a diameter of 40 to 100 nanometers. All cultured cell types can secrete exosomes, and exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid, and breast milk. Traditional stem cell exosome centrifugation is usually done manually. During the operation, it is often difficult to accurately control the centrifugation speed, and exosomes are often not separated well. A second manual operation is often required, which wastes a lot of manpower and resources.

[0003] For example, the utility model patent with authorization announcement number CN 112705362 A discloses a centrifuge device for processing stem cell exosomes, which includes a device body, an installation plate fixedly installed on one side wall of the device body, a second motor fixedly installed on the installation plate, a gear fixedly connected to the rotating part of the second motor, a support fixedly installed inside the device body, a fixed cylinder rotatably installed on the support, a gear ring fixedly installed on the outer surface of the fixed cylinder, a first motor fixedly installed on the top of the device body, and a first bevel gear fixedly installed on the rotating part of the first motor. However, the mechanism for processing secretions is simple and cannot guarantee the centrifugation effect. In view of this, a centrifuge device for processing stem cell exosomes is proposed. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a centrifuge device for processing stem cell exosomes.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A centrifuge device for processing stem cell exosomes includes a base, a side plate fixedly connected to the top outer wall of the base, a support plate provided on the outer wall of the side plate, a processing chamber rotatably connected to the support plate, a first centrifugation mechanism provided between the processing chamber and the side plate, a second centrifugation mechanism provided on the outer wall of the processing chamber, a top cover threadedly connected to the top of the processing chamber, a discharge pipe fixedly connected to the bottom of the processing chamber, a discharge valve fixedly connected to the outer wall of the discharge pipe, a crushing mechanism provided on the inner wall of the processing chamber, a filtering mechanism provided at the bottom of the inner wall of the processing chamber, an aeration mechanism provided on the inner wall of the processing chamber, and a feed cover plate rotatably connected to one side outer wall of the processing chamber.

[0007] Preferably, the first centrifugal mechanism includes a rotating plate and a second motor, the second motor and the side plate are fixedly connected, the rotating plate is located on one side of the support plate, and the rotating plate and the second motor are fixedly connected.

[0008] Preferably, a plurality of first springs and limiting telescopic rods are fixedly connected between the rotating plate and the support plate, and a plurality of vibration motors are fixedly connected to the outer wall of the support plate.

[0009] Preferably, the second centrifugal mechanism includes a drive gear and a driven gear. The driven gear is fixedly connected to the processing box, the drive gear is drivenly connected to both ends of the driven gear, and a first motor is fixedly connected to the bottom of the drive gear. The first motor is fixedly connected to the support plate.

[0010] Preferably, the crushing mechanism includes a third motor and a rotating shaft. The bottom of the third motor is fixedly connected to the rotating shaft. Multiple crushing blades are fixedly connected to the outer wall of the rotating shaft. The crushing blades are located inside the processing box. Multiple connecting rods are fixedly connected to the outer wall of the rotating shaft. A scraper is fixedly connected to the other end of the connecting rod. The scraper is in natural contact with the inner wall of the processing box.

[0011] Preferably, the aeration mechanism includes an air pump and an air guide box, the air guide box and the top cover are fixedly connected, the third motor and the air guide box are fixedly connected, the air pump and the outer wall of the air guide box are fixedly connected, the rotating shaft is hollow, the outer wall of the rotating shaft has an air guide hole, the air guide hole is located inside the air guide box, and multiple aeration pipes are fixedly connected to the outer wall of the rotating shaft, the aeration pipes are located inside the treatment box.

[0012] Preferably, the filtration mechanism includes a filter plate and a fixing ring, the fixing ring being fixedly connected to the inner wall of the processing box, and a plurality of second springs being fixedly connected to the top of the fixing ring, the tops of the second springs being fixedly connected to the filter plate.

[0013] The beneficial effects of this utility model are as follows:

[0014] 1. By setting up a crushing mechanism inside the processing box, the raw materials inside the processing box can be agitated, crushed, and scraped. By setting up an aeration mechanism inside the processing box, the raw materials inside the processing box can be aerated, further improving the crushing and centrifugation effect. By setting up a second centrifugation mechanism on the outer wall of the processing box, the processing box can have horizontal centrifugation capability. By setting up a first centrifugation mechanism, the processing box can have vertical centrifugation capability, ensuring that the raw materials inside the processing box can be centrifuged efficiently.

[0015] 2. By setting a first spring and a limiting telescopic rod between the support plate and the rotating plate, the processing box can be made movable. By setting a vibration motor on the outer wall of the support plate, the processing box can be made to have high-frequency vibration capability, thereby improving the crushing and centrifugation effect. Attached Figure Description

[0016] Figure 1This is a schematic diagram of the main structure of a centrifuge device for processing stem cell exosomes proposed in this utility model;

[0017] Figure 2 This is a schematic diagram of the back structure of a centrifuge device for processing stem cell exosomes proposed in this utility model;

[0018] Figure 3 This is a cross-sectional structural diagram of a centrifuge device for processing stem cell exosomes proposed in this utility model;

[0019] Figure 4 for Figure 3 Enlarged structural diagram at point A in the middle.

[0020] In the diagram: 1. Base, 2. Side plate, 3. Rotating plate, 4. First spring, 5. Limiting telescopic rod, 6. Top cover, 7. Vibration motor, 8. Support plate, 9. Feed cover plate, 10. Processing box, 11. Driven gear, 12. Drive gear, 13. First motor, 14. Discharge valve, 15. Discharge pipe, 16. Second motor, 17. Connecting rod, 18. Scraper, 19. Rotating shaft, 20. Crushing blade, 21. Second spring, 22. Fixing ring, 23. Filter plate, 24. Air guide hole, 25. Air pump, 26. Air guide box, 27. Third motor, 28. Aeration pipe. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0022] Reference Figure 1-4 A centrifuge device for processing stem cell exosomes includes a base 1, a side plate 2 fixedly connected to the top outer wall of the base 1, a support plate 8 provided on the outer wall of the side plate 2, a processing chamber 10 rotatably connected to the support plate 8, a first centrifugation mechanism provided between the processing chamber 10 and the side plate 2, a second centrifugation mechanism provided on the outer wall of the processing chamber 10, a top cover 6 threadedly connected to the top of the processing chamber 10, a discharge pipe 15 fixedly connected to the bottom of the processing chamber 10, a discharge valve 14 fixedly connected to the outer wall of the discharge pipe 15, a crushing mechanism provided on the inner wall of the processing chamber 10, a filtering mechanism provided at the bottom of the inner wall of the processing chamber 10, and a... The treatment box 10 is equipped with an aeration mechanism, and a feed cover plate 9 is rotatably connected to one side of the outer wall. The crushing mechanism inside the treatment box 10 can agitate, crush, and scrape the raw materials inside the treatment box 10. The aeration mechanism inside the treatment box 10 can aerate the raw materials inside the treatment box 10, further improving the crushing and centrifugation effect. The second centrifugation mechanism on the outer wall of the treatment box 10 can enable the treatment box 10 to have horizontal centrifugation capability. The first centrifugation mechanism can enable the treatment box 10 to have vertical centrifugation capability, ensuring that the raw materials inside the treatment box 10 can be centrifuged efficiently.

[0023] In this utility model, the first centrifugal mechanism includes a rotating plate 3 and a second motor 16. The second motor 16 and the side plate 2 are fixedly connected. The rotating plate 3 is located on one side of the support plate 8. The rotating plate 3 and the second motor 16 are fixedly connected. By setting the first centrifugal mechanism, the processing box 10 can have vertical centrifugal capability, ensuring that the raw materials in the processing box 10 can be centrifuged efficiently.

[0024] Multiple first springs 4 and limiting telescopic rods 5 are fixedly connected between the rotating plate 3 and the support plate 8. Multiple vibration motors 7 are fixedly connected to the outer wall of the support plate 8. By setting the first springs 4 and limiting telescopic rods 5 between the support plate 8 and the rotating plate 3, the processing box 10 can be movable. By setting the vibration motors 7 on the outer wall of the support plate 8, the processing box 10 can have high-frequency vibration capability, thereby improving the crushing and centrifugation effect.

[0025] The second centrifugal mechanism includes a drive gear 12 and a driven gear 11. The driven gear 11 is fixedly connected to the processing box 10. The drive gear 12 is connected to both ends of the driven gear 11. The drive gear 12, by setting the second centrifugal mechanism on the outer wall of the processing box 10, can enable the processing box 10 to have a horizontal centrifugal capability.

[0026] The crushing mechanism includes a third motor 27 and a rotating shaft 19. The bottom of the third motor 27 is fixedly connected to the rotating shaft 19. Multiple crushing blades 20 are fixedly connected to the outer wall of the rotating shaft 19. The crushing blades 20 are located inside the processing box 10. Multiple connecting rods 17 are fixedly connected to the outer wall of the rotating shaft 19. A scraper 18 is fixedly connected to the other end of the connecting rod 17. The scraper 18 is in natural contact with the inner wall of the processing box 10. By setting the crushing mechanism inside the processing box 10, the raw materials inside the processing box 10 can be agitated, crushed, and scraped.

[0027] The aeration mechanism includes an air pump 25 and an air guide box 26. The air guide box 26 is fixedly connected to the top cover 6. The third motor 27 is fixedly connected to the air guide box 26. The air pump 25 is fixedly connected to the outer wall of the air guide box 26. The rotating shaft 19 is hollow. An air guide hole 24 is opened on the outer wall of the rotating shaft 19. The air guide hole 24 is located inside the air guide box 26. Multiple aeration pipes 28 are fixedly connected to the outer wall of the rotating shaft 19. The aeration pipes 28 are located inside the processing box 10. By setting the aeration mechanism in the processing box 10, the raw materials in the processing box 10 can be aerated to further improve the crushing and centrifugation effect.

[0028] The filtration mechanism includes a filter plate 23 and a fixing ring 22. The fixing ring 22 is fixedly connected to the inner wall of the processing box 10. Multiple second springs 21 are fixedly connected to the top of the fixing ring 22. The top of the second springs 21 is fixedly connected to the filter plate 23, which can filter the material after centrifugation.

[0029] Working Principle: In the idle area of ​​this device, all the components mentioned above, which refer to structural parts, are connected. The specific connection method should refer to the working principle described below, and the connection is completed in the order of operation of each component. The detailed connection method is a well-known technology in this field. The following mainly introduces the working principle and process, and will not explain it further. When using this device, the crushing mechanism in the processing box 10 can be set to agitate, crush and scrape the raw materials in the processing box 10. The aeration mechanism in the processing box 10 can be set to aerate the raw materials in the processing box 10, further improving the crushing and centrifugation effect. The second centrifugation mechanism on the outer wall of the processing box 10 can make the processing box 10 have horizontal centrifugation ability. The first centrifugation mechanism can make the processing box 10 have vertical centrifugation ability, ensuring that the raw materials in the processing box 10 can be centrifuged efficiently.

[0030] The above are merely preferred embodiments of this utility model, but the scope of protection of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this utility model, based on the technical solution and inventive concept of this utility model, should be included within the scope of protection of this utility model.

Claims

1. A centrifugation device for stem cell exosome processing comprising a base (1), characterized in that, The outer wall of the side plate (2) is provided with a support plate (8), the support plate (8) is rotatably connected with a processing box (10), a first centrifugal mechanism is arranged between the processing box (10) and the side plate (2), the outer wall of the processing box (10) is provided with a second centrifugal mechanism, the top of the processing box (10) is threadedly connected with a top cover (6), the bottom of the processing box (10) is fixedly connected with a discharge pipe (15), the outer wall of the discharge pipe (15) is fixedly connected with a discharge valve (14), the inner wall of the processing box (10) is provided with a crushing mechanism, the inner wall of the processing box (10) is provided with a filtering mechanism, the inner wall of the processing box (10) is provided with an aeration mechanism, and the outer wall of one side of the processing box (10) is rotatably connected with a feeding cover plate (9).

2. The centrifuge device for processing stem cell exosomes according to claim 1, characterized in that, The first centrifugal mechanism comprises a rotating plate (3) and a second motor (16), the second motor (16) is fixedly connected with the side plate (2), and the rotating plate (3) is located on one side of the support plate (8) and is fixedly connected with the second motor (16).

3. The centrifuge device for processing stem cell exosomes according to claim 2, characterized in that, A plurality of first springs (4) and limiting telescopic rods (5) are fixedly connected between the rotating plate (3) and the support plate (8), and a plurality of vibration motors (7) are fixedly connected to the outer wall of the support plate (8).

4. The centrifuge device for processing stem cell exosomes according to claim 1, wherein, The second centrifugal mechanism comprises a driving gear (12) and a driven gear (11), the driven gear (11) is fixedly connected with the processing box (10), the driving gear (12) is in transmission connection with the two ends of the driven gear (11) respectively, the bottom of the driving gear (12) is fixedly connected with a first motor (13), and the first motor (13) is fixedly connected with the support plate (8).

5. The centrifuge device for processing stem cell exosomes according to claim 1, wherein, The crushing mechanism comprises a third motor (27) and a rotating shaft (19), the bottom of the third motor (27) is fixedly connected with the rotating shaft (19), a plurality of crushing knives (20) are fixedly connected to the outer wall of the rotating shaft (19), the crushing knives (20) are located in the processing box (10), a plurality of connecting rods (17) are fixedly connected to the outer wall of the rotating shaft (19), the other ends of the connecting rods (17) are fixedly connected with scrapers (18), and the scrapers (18) are in natural contact with the inner wall of the processing box (10).

6. The centrifuge device for processing stem cell exosomes according to claim 5, wherein, The aeration mechanism comprises an air pump (25) and a gas guide box (26), the gas guide box (26) is fixedly connected with the top cover (6), the third motor (27) is fixedly connected with the gas guide box (26), the air pump (25) is fixedly connected to the outer wall of the gas guide box (26), the rotating shaft (19) is hollow, the outer wall of the rotating shaft (19) is provided with a gas guide hole (24), the gas guide hole (24) is located in the gas guide box (26), and a plurality of aeration pipes (28) are fixedly connected to the outer wall of the rotating shaft (19).

7. The centrifuge device for processing stem cell exosomes according to claim 1, wherein, The filtering mechanism comprises a filtering plate (23) and a fixed ring (22), the fixed ring (22) is fixedly connected with the inner wall of the processing box (10), a plurality of second springs (21) are fixedly connected to the top of the fixed ring (22), and the top of the second spring (21) is fixedly connected with the filtering plate (23).

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