Pressure-adjustable cassava leaf pathogen inoculator

Abstract

The utility model relates to the technical field of plant pathology research, and discloses a pressure-adjustable cassava leaf germ inoculator which comprises a box body and a gun body placed in the box body, the inner wall of the gun body is fixedly connected with a first clamping block and a second clamping block to fix an injector, and the inner wall of the gun body is fixedly connected with a second sliding rail and a second electric push rod. A first sliding rail is fixedly connected to the bottom end of the gun body, a supporting frame is slidably connected into the first sliding rail, the first electric push rod drives the supporting frame to move along the first sliding rail, a pressure sensor and a soft cushion are fixedly connected to the outer wall of the supporting frame, and the soft cushion corresponds to an injection opening of the injector. The second electric push rod is matched with the second sliding rail to achieve automatic quantitative inoculation, the pressure sensor and the soft cushion are matched with the first electric push rod to achieve pressure closed-loop adjustment, cassava leaves are effectively prevented from being damaged, and inoculation efficiency is remarkably improved.

Description

Technical Field

[0001] This utility model relates to the field of plant pathology research technology, and in particular to an adjustable pressure cassava leaf pathogen inoculator. Background Technology

[0002] In the process of identifying resistance to cassava diseases and conducting pathological research, it is necessary to carry out experiments on artificial inoculation of pathogens. The injection inoculation method is used to inject the suspension of pathogens into the mesophyll tissue of cassava leaves. The existing inoculation method mainly relies on manual operation with a hand-held syringe. The experimenter needs to support the cassava leaf with one hand and hold the syringe with the other hand, keeping the tip of the syringe close to the back of the leaf. Then, the syringe plunger is pushed to press the bacteria into the leaf tissue. After the injection, the leaf continues to grow on the plant. A few days later, small leaflets are cut off for photographing the area of ​​lesions or for conducting experiments.

[0003] When conducting large-scale germplasm resource screening or batch inoculation experiments, this purely manual operation method has obvious limitations. Because it requires repeated pushing of the syringe plunger for a long time, it leads to hand fatigue of the experimenter, which significantly reduces the inoculation efficiency. Manual operation makes it difficult to accurately control the pressure of the syringe tip on the cassava leaf. Cassava leaves are brittle and thin. If the experimenter uses too much force, the syringe tip may damage or even perforate the leaf tissue, causing non-pathological necrosis at the inoculation site and interfering with the accurate determination of experimental results. If the force is too little, the syringe tip will not fit tightly against the leaf surface, causing the bacterial solution to flow out from the gaps and fail to enter the leaf tissue, resulting in inoculation failure.

[0004] Therefore, this invention proposes an adjustable pressure cassava leaf pathogen inoculator to address the shortcomings of existing technologies. Utility Model Content

[0005] In view of the shortcomings of existing technologies that mainly rely on manual hand-held syringe operation, such as low inoculation efficiency, hand fatigue caused by long-term operation, difficulty in accurately controlling the injection force leading to damage to cassava leaves, and inoculation failure due to poor adhesion, this utility model aims to provide an adjustable pressure cassava leaf pathogen inoculator with an improved structure that can effectively solve the above problems.

[0006] This utility model provides an adjustable pressure cassava leaf pathogen inoculator, including a box body, a gun body inside the box body, a locking block one fixedly connected to the inner wall of the gun body, a locking block two fixedly connected to the inner wall of the gun body, a syringe provided on the inner wall of the locking block one, and the rolled edge handle of the syringe being engaged with the inner wall of the locking block two.

[0007] The gun body has two slide rails 2 fixedly connected to its inner wall, and a fixed block is slidably connected to the inner wall of the two slide rails 2. The end of the syringe plunger is placed on the inner wall of the fixed block. An electric push rod 2 is installed on the inner wall of the gun body. The output end of the electric push rod 2 is fixedly connected to the outer wall of the fixed block. A slide rail 1 is fixedly connected to the bottom of the gun body. A support frame is slidably connected to the inner wall of the slide rail 1. An electric push rod 1 is fixedly connected to the right end of the support frame. The outer wall of the electric push rod 1 is fixedly connected to the inner wall of the gun body. A pressure sensor is fixedly connected to the outer wall of the support frame. A soft pad is fixedly connected to the outer wall of the pressure sensor. The soft pad is set at the position of the corresponding syringe injection port.

[0008] Preferably, a control system is installed on the inner wall of the gun body. The control system is electrically connected to electric push rod one, electric push rod two, and pressure sensor respectively. The control system realizes centralized control of each electric component and processing of pressure signals.

[0009] Preferably, a button is fixedly connected to the outer wall of the gun body. The button is electrically connected to the control system and is used to send a signal to control the start and stop of the electric push rod, so that the user can operate the control support frame to move with one hand.

[0010] Preferably, a second button is fixedly connected to the outer wall of the gun body. The second button is electrically connected to the control system. The second button is used to send a signal to control the start and stop of the electric push rod, so that the user can control the inoculation action after confirming that the blade is fixed.

[0011] Preferably, a placement platform is fixedly connected to the inner wall of the box, and two fixing clips are rotatably connected to the top of the placement platform. The fixing clips are used to press the cassava leaves placed on the placement platform, thereby providing a stable support platform for subsequent photo recording.

[0012] Preferably, the support frame is a U-shaped structure, with the bottom end of the support frame slidably connected to the inner wall of the slide rail, and the pressure sensor fixedly connected to the vertical side of the support frame. The U-shaped structure can effectively avoid the space under the syringe and provide vertical extrusion force.

[0013] Preferably, the outer walls of the fixed block are provided with grooves on both sides that cooperate with the two slide rails. The fixed block moves back and forth in a straight line between the two slide rails through the grooves. The cooperation between the grooves and the slide rails restricts the degree of freedom of the fixed block to ensure the straightness of the injection direction.

[0014] Preferably, the pad is made of a micro-elastic material, and the surface area of ​​the pad is larger than the cross-sectional area of ​​the syringe injection port. The elastic deformation of the pad buffers the rigid impact of the injection port on the blade and increases the force-bearing area.

[0015] Preferably, the control system includes a microprocessor and a power module. The control system is configured to receive pressure signals from a pressure sensor and adjust the advance stroke of the electric push rod, thereby automatically stopping the clamping action based on real-time pressure feedback to protect the blade.

[0016] This utility model has the following beneficial effects:

[0017] 1. This utility model solves the problems of slow manual injection speed, hand fatigue, and uneven injection force in the existing cassava leaf inoculation process by setting an electric push rod 2 inside the gun body in conjunction with a slide rail 2 to push the fixed block and the syringe core rod to move linearly. It achieves the effects of realizing automated quantitative inoculation, significantly improving inoculation efficiency, reducing labor intensity, and ensuring consistent inoculation force.

[0018] 2. This utility model solves the problems of cassava leaves being crushed and damaged due to improper pressure control and leakage due to poor adhesion during inoculation by setting soft pads and pressure sensors on the support frame and using the control system to adjust the extension and retraction stroke of the electric push rod one according to pressure feedback. It achieves the effects of accurately controlling the clamping force, using soft contact buffer to protect the integrity of cassava leaves, and ensuring reliable sealing of the inoculation port.

[0019] 3. This utility model solves the problem of inconvenience caused by the lack of a fixing device when taking pictures of the cut leaflets with disease spots after the inoculation experiment by setting a placement platform on the inner wall of the box and rotating and connecting the fixing clamp on the placement platform. It achieves the effects of making full use of the box space, providing a standardized shooting and sampling platform, and facilitating users to record experiments. Attached Figure Description

[0020] Figure 1 A perspective view of an adjustable pressure cassava leaf pathogen inoculator proposed in this utility model;

[0021] Figure 2 This is a schematic diagram of the gun body of an adjustable pressure cassava leaf pathogen inoculator proposed in this utility model;

[0022] Figure 3 This is a top cross-sectional view of the gun body of an adjustable pressure cassava leaf pathogen inoculator proposed in this utility model.

[0023] Figure 4 This is a cross-sectional view of the gun body of an adjustable pressure cassava leaf pathogen inoculator proposed in this utility model.

[0024] Figure 5 This is a split view of the syringe of an adjustable pressure cassava leaf pathogen inoculator proposed in this utility model;

[0025] Figure 6 This is a split view of the pad of an adjustable pressure cassava leaf pathogen inoculator proposed in this utility model.

[0026] Legend:

[0027] 1. Housing; 2. Gun body; 3. Syringe; 4. Locking block one; 5. Locking block two; 6. Fixing block; 7. Slide rail one; 8. Support frame; 9. Button one; 10. Control system; 11. Electric push rod one; 12. Soft pad; 13. Button two; 14. Electric push rod two; 15. Slide rail two; 16. Pressure sensor; 17. Placement platform; 18. Fixing clamp. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the protection scope of this utility model. Example

[0029] Please refer to Figures 1 to 6 This utility model provides an adjustable pressure cassava leaf pathogen inoculator, which aims to solve the problems of slow manual operation, hand fatigue, and cassava leaf damage due to improper injection force control in existing cassava leaf inoculation technology, which leads to inoculation failure.

[0030] Please refer to Figure 1 and Figure 2 An adjustable pressure cassava leaf pathogen inoculator includes a housing 1, which serves as the storage carrier for the entire device. A gun body 2 is housed inside the housing 1, acting as the main structure for handheld operation. In the non-working state, the gun body 2 is stored inside the housing 1; in the working state, it is removed by the user for handheld operation. To accommodate a container for holding the bacterial solution, a locking block 4 and a locking block 5 are fixedly connected to the inner wall of the gun body 2. The injection port of the syringe 3 is supported and abuts against the inner wall of the locking block 4, and the rolled edge handle of the syringe 3 is engaged with the inner wall of the locking block 5. The cooperation of the locking blocks 4 and 5 securely restricts the position of the outer cylinder of the syringe 3, preventing displacement during injection. Furthermore, the syringe 3 is a disposable item, avoiding cross-contamination between different bacterial solutions and ensuring the reliability of the experiment.

[0031] Please refer to Figure 3 , Figure 4 and Figure 5Two slide rails 15 are fixedly connected to the inner wall of the gun body 2. A fixing block 6 is slidably connected to the inner wall of the two slide rails 15. The end of the syringe rod is placed and fixed to the inner wall of the fixing block 6. In order to provide stable injection power, an electric push rod 14 is provided on the inner wall of the gun body 2. The output end of the electric push rod 14 is fixedly connected to the outer wall of the fixing block 6. Slide grooves that cooperate with the two slide rails 15 are respectively opened on both sides of the outer wall of the fixing block 6. The fixing block 6 moves back and forth in a straight line between the two slide rails 15 through the slide grooves. When the electric push rod 14 is started, it pushes the fixing block 6 to move smoothly along the direction of the slide rails 15, thereby driving the syringe rod of the syringe 3 to perform a precise pushing motion in the syringe 3 barrel to complete the quantitative injection of liquid.

[0032] Please refer to Figure 2 , Figure 4 and Figure 6 The bottom end of the gun body 2 is fixedly connected to a slide rail 7, which provides a guide for the movement of the support components. A support frame 8 is slidably connected to the inner wall of the slide rail 7. The support frame 8 has a U-shaped structure, is fully vertical, and ensures no pressure deviation, preventing excessive local pressure and damage to the blades. The bottom end of the support frame 8 is slidably connected to the inner wall of the slide rail 7. To achieve automated movement of the support frame 8, an electric push rod 11 is fixedly connected to the right end of the support frame 8. The outer wall of the electric push rod 11 is fixedly connected to the inner wall of the gun body 2. The telescopic end of the electric push rod 11 drives the support frame 8 to reciprocate along the length of the slide rail 7, thereby changing the distance between the support frame 8 and the injection port of the syringe 3. To provide buffer protection for the cassava leaves and achieve precise pressure sensing, the outer wall of the support frame 8... A pressure sensor 16 is fixedly connected, and a soft pad 12 is fixedly connected to the outer wall of the pressure sensor 16. The soft pad 12 is positioned at the injection port of the corresponding syringe 3. During the inoculation process, the cassava leaf is placed between the soft pad 12 and the injection port of the syringe 3. The soft pad 12 is made of elastic rubber material, and its surface area is larger than the cross-sectional area of ​​the injection port of the syringe 3, so that the leaf around the injection point is not damaged. The soft pad 12 can prevent the injection port of the syringe 3 from making hard contact with the cassava leaf and causing damage. The soft pad 12 fits closely to the injection port, so that there is no leakage between the leaf and the injection port during injection. At the same time, the pressure generated between the soft pad 12 and the injection port should not be too large to prevent the leaf from breaking. Then, the pressure sensor 16 can detect the contact pressure data in real time when the soft pad 12 presses the cassava leaf.

[0033] In a preferred embodiment, a control system 10 is fixedly installed on the inner wall of the gun body 2. The control system 10 is electrically connected to the electric push rod 11, the electric push rod 14 and the pressure sensor 16. The control system 10 integrates a microprocessor and a power module. The control system 10 is configured to receive the pressure signal fed back by the pressure sensor 16 in real time, and automatically adjust the pushing stroke and stopping position of the electric push rod 11 according to the preset pressure threshold through an algorithm, thereby effectively preventing physical damage to the cassava leaves due to excessive clamping force.

[0034] In another preferred embodiment, a button 9 is fixedly connected to the outer wall of the gun body 2, and a button 13 is fixedly connected to the outer wall of the gun body 2. Button 9 is electrically connected to the control system 10 and serves as an operating switch to control the start and stop of the electric push rod 11. Button 13 is electrically connected to the control system 10 and serves as an operating switch to control the start and stop of the electric push rod 14. By pressing three times in succession, the injection mode is entered. After that, a light press is made to apply a pushing force to the device, so as to inject the bacterial solution into the leaf. The user can press button 9 and button 13 with their fingers to complete the clamping of the leaf and the automatic injection of the bacterial solution in sequence. Moreover, the stability of electronic control is stronger than that of manual injection, avoiding large differences in the injection force and causing large differences in the disease effect at the injection site of the leaf.

[0035] As another preferred embodiment, a placement platform 17 is fixedly connected to the inner wall of the box 1. Two fixing clips 18 are rotatably connected to the top of the placement platform 17. After the inoculation experiment is completed, the user lays the cut cassava leaf sample flat on the top surface of the placement platform 17 and presses the edge of the cassava leaf by rotating the two fixing clips 18, thereby ensuring the flatness and stability of the leaf during the photography process.

[0036] Working principle: When inoculating cassava leaves, the user first opens the cover of box 1 and takes out gun body 2 from inside box 1. Then, the user holds the handle of gun body 2 and installs syringe 3 containing the inoculation solution on the top of gun body 2. Specifically, the injection port end of syringe 3 is inserted into and abuts against the inner wall of locking block 4, the rolled edge handle of syringe 3 is inserted into the inner wall of locking block 5, and the core rod end of syringe 3 is embedded and fixed in the inner wall of fixing block 6. Through the cooperation and connection of syringe 3 with locking block 4, locking block 5 and fixing block 6, the syringe 3 is stably installed on gun body 2.

[0037] After installation, the user places the cassava leaf to be inoculated between the support frame 8 and the injection port of the syringe 3, and then presses the button 9 located on the outer wall of the gun body 2. After receiving the start signal from the button 9, the control system 10 controls the electric push rod 11 to start. The telescopic rod of the electric push rod 11 extends and pushes the support frame 8 to slide along the inner wall of the slide rail 7 towards the syringe 3. The support frame 8 drives the pressure sensor 16 and the soft pad 12 to gradually approach and press the cassava leaf. During the process of the soft pad 12 contacting the cassava leaf and generating squeezing force, the pressure sensor 16 detects the pressure value in real time and transmits the signal to the control system 10. When the pressure reaches the preset safety threshold, the control system 10 immediately controls the electric push rod 11 to stop extending. At this time, the soft pad 12 and the injection port of the syringe 3 clamp the cassava leaf with appropriate force, which not only ensures the sealing of the inoculation position, but also effectively avoids damage to the cassava leaf due to excessive pressure by utilizing the buffering property of the soft pad 12 and the closed-loop control of the pressure sensor 16.

[0038] After the cassava leaves are securely fixed, the user presses button 13 on the outer wall of the gun body 2. Upon receiving the signal, the control system 10 activates the electric push rod 14. The electric push rod 14 pushes the fixing block 6 forward smoothly along the guide direction of the two slide rails 15. The fixing block 6 drives the core rod of the syringe 3 to advance at a constant speed inside the syringe 3, injecting the bacterial solution into the back tissue of the cassava leaves through the injection port, thus completing the automated inoculation. At the same time, the pressure sensor 16 detects the pressure value in real time and transmits the signal to the control system 10. The control system 10 controls the electric push rod 11 to adjust the position of the support frame 8 according to the pressure. After inoculation, the user presses button 9 again. The control system 10 controls the electric push rod 11 to retract, and the support frame 8 drives the soft pad 12 to move in the opposite direction and release the cassava leaves. The user can then remove the gun body 2 to perform the inoculation operation on the next leaf. If it is necessary to record the small leaf with disease spots that have been cut off, the user lays the small leaf flat on the placement platform 17 inside the box 1, rotates the fixing clamp 18 to press down the edge of the leaf, and then takes a stable picture for sampling.

Claims

1. An adjustable pressure cassava leaf blight inoculator, comprising a box (1), the inside of the box (1) is provided with a gun body (2), the inner wall of the gun body (2) is fixedly connected with a clamping block one (4), the inner wall of the gun body (2) is fixedly connected with a clamping block two (5), the inner wall of the clamping block one (4) is provided with a syringe (3), the crimped handle of the syringe (3) is clamped in the inner wall of the clamping block two (5); characterized in that The inner wall of the gun body (2) is fixedly connected with two slide rails two (15), the inner wall of the two slide rails two (15) is slidably connected with a fixed block (6), the inner wall of the gun body (2) is provided with an electric push rod two (14), the output end of the electric push rod two (14) is fixedly connected with the outer wall of the fixed block (6), the bottom end of the gun body (2) is fixedly connected with a slide rail one (7), the inner wall of the slide rail one (7) is slidably connected with a support frame (8), the right end of the support frame (8) is fixedly connected with an electric push rod one (11), the outer wall of the support frame (8) is fixedly connected with a pressure sensor (16), the outer wall of the pressure sensor (16) is fixedly connected with a soft pad (12).

2. The adjustable pressure cassava leaf blight inoculator according to claim 1, wherein, The inner wall of the gun body (2) is provided with a control system (10), the control system (10) is electrically connected with the electric push rod one (11), the electric push rod two (14) and the pressure sensor (16) respectively.

3. A pressure-adjustable Colletotrichum leaf blight inoculator according to claim 2, characterized in that, The outer wall of the gun body (2) is fixedly connected with a button one (9), the button one (9) is electrically connected with the control system (10).

4. The adjustable pressure cassava leaf blight inoculator according to claim 2, wherein, The outer wall of the gun body (2) is fixedly connected with a button two (13), the button two (13) is electrically connected with the control system (10).

5. The adjustable pressure cassava leaf blight inoculator of claim 1, wherein, The inner wall of the box (1) is fixedly connected with a placing table (17), the top end of the placing table (17) is rotatably connected with two fixed clamps (18).

6. The adjustable pressure cassava leaf blight inoculator of claim 1, wherein, The support frame (8) is a U-shaped structure, the bottom end of the support frame (8) is slidably connected with the inner wall of the slide rail one (7), the vertical surface of the support frame (8) is fixedly connected with the pressure sensor (16).

7. The adjustable pressure cassava leaf blight inoculator of claim 1, wherein, The outer wall of the fixed block (6) is provided with a sliding groove on both sides, which matches with the two slide rails two (15), the fixed block (6) moves linearly back and forth between the two slide rails two (15) through the sliding groove.

8. The adjustable pressure cassava leaf blight inoculator of claim 1, wherein, The soft pad (12) is made of micro-elastic material, the surface area of the soft pad (12) is greater than the cross-sectional area of the injection port of the syringe (3).

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