Degradable biological scaffold assisted dairy cow embryo three-dimensional culture system
The biodegradable scaffold-assisted three-dimensional culture system solves the problem that traditional planar culture cannot simulate the uterine environment, improves the success rate of embryo development, reduces costs, and achieves flexibility and independence in multi-stage embryo culture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-28
- Publication Date
- 2026-03-13
AI Technical Summary
Traditional planar culture technology cannot fully simulate the complex environment of the uterus, resulting in restricted embryo development. Furthermore, existing technologies require changing the culture medium at different growth stages, which increases costs.
A biodegradable scaffold-assisted three-dimensional culture system, including a microfluidic chip, hollow fiber tube, and gas regulation device, simulates the three-dimensional environment inside the uterus and achieves multi-stage embryo culture through modular design.
It improves the success rate of embryonic cell growth and development, reduces culture costs, and enables flexibility and independence in multi-stage embryo culture.
Smart Images
Figure CN223991109U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of cell culture, and more specifically, to a biodegradable bioscaffold-assisted three-dimensional culture system for dairy cow embryos. Background Technology
[0002] Traditional planar embryo culture technology has made significant progress in laboratory settings, but it still cannot fully simulate the complex environment of the uterus. In reality, the uterus not only provides nutrients and oxygen but also creates unique three-dimensional spatial and biochemical conditions for embryonic development through dynamic mechanical forces, hormonal regulation, and intercellular interactions. Planar culture, lacking these key factors, restricts embryonic development, making it difficult to achieve the same developmental potential as natural pregnancy. Furthermore, current techniques require changing the culture medium at different stages of embryonic development. This involves removing the oocytes and transferring them into a pre-balanced culture medium, which undoubtedly increases culture costs. Therefore, although embryo culture technology has significant value in research and assisted reproductive technologies, further breakthroughs are needed in the three-dimensional space aspect. Utility Model Content
[0003] To address the aforementioned problems in embryo culture, this application provides a biodegradable bioscaffold-assisted three-dimensional embryo culture system. This system better simulates the uterine environment, improving the success rate of embryo culture and the level of healthy embryo development.
[0004] The technical solution of this application is as follows:
[0005] 1. A biodegradable bioscaffold-assisted three-dimensional embryo culture system, characterized in that the culture system comprises:
[0006] Culture medium, microfluidic chip, hollow fiber tube, gas conditioning device;
[0007] The culture body includes: an inlet, an outlet, a culture chamber, a liquid inlet channel, and a liquid outlet channel;
[0008] The microfluidic chip, the hollow fiber tube, and the culture medium are connected in sequence in the direction of liquid flow, and the gas regulating device is connected to the hollow fiber tube.
[0009] 2. The culture system according to item 1, characterized in that,
[0010] In the culture body, the inlet is connected to one end of the liquid inlet channel, the other end of the liquid inlet channel is connected to the culture chamber, the culture chamber is also connected to one end of the liquid outlet channel, and the other end of the liquid outlet channel is connected to the outlet.
[0011] 3. The culture system according to item 1, characterized in that,
[0012] A biomimetic scaffold is placed in the culture chamber, and the volume of the culture chamber is larger than the volume occupied by the biomimetic scaffold.
[0013] 4. The culture system according to item 3, characterized in that,
[0014] The biomimetic scaffold has a porous structure, and the surface of the biomimetic scaffold is coated with cells to be cultured. The porosity of the biomimetic scaffold is greater than 90%.
[0015] 5. The culture system according to item 1, characterized in that,
[0016] Along the depth of the culture chamber, the inlet channel is located below the outlet channel.
[0017] 6. The culture system according to item 1, characterized in that,
[0018] The culture medium is also provided with a sealing cap, which is adapted to the culture chamber.
[0019] 7. The culture system according to item 1, characterized in that,
[0020] The hollow fiber tube includes a hollow fiber tube channel and a gas channel, and the hollow fiber tube channel has holes.
[0021] 8. The culture system according to item 1, characterized in that,
[0022] The culture system includes multiple hollow fiber tubes and multiple microfluidic chips. The multiple hollow fiber tubes are respectively connected to the multiple microfluidic chips, and all of the multiple hollow fiber tubes are connected to the culture body.
[0023] 9. The culture system according to item 8, characterized in that,
[0024] The culture medium has multiple inlets, the number of which is equal to the number of hollow fiber tubes.
[0025] 10. The culture system according to item 1, characterized in that,
[0026] The culture system is connected to a computer program, automated equipment, or circuit board.
[0027] The beneficial effects of this application are as follows:
[0028] The culture system provided in this application can simulate the three-dimensional environment inside the uterus through a biodegradable scaffold and a gas control device, which can help embryonic cells grow and develop normally and increase the blastocyst conversion rate.
[0029] The culture system provided in this application enables multiple stages of embryo culture to be completed in a single culture chamber by connecting multiple microfluidic chips in parallel. At the same time, the modular design allows those skilled in the art to flexibly adjust the various modules of embryo culture, with each module being independent of the others, providing a high degree of freedom and flexibility. Attached Figure Description
[0030] The accompanying drawings are provided to better understand this application and do not constitute an undue limitation thereof. Wherein:
[0031] Figure 1 This is a top view of one embodiment of the cultivation subject of this application.
[0032] Figure 2 This is a cross-sectional schematic diagram of the culture subject of this application.
[0033] Figure 3 This is a schematic diagram of the hollow fiber tube structure in this application.
[0034] Figure 4 This is a schematic diagram of the microfluidic chip of this application.
[0035] Figure 5 This is a schematic diagram of one implementation of the cultivation system of this application.
[0036] Figure 6 This is a schematic diagram of one implementation of the cultivation system of this application.
[0037] Among them, 1 is the culture body, 2 is the inlet, 3 is the liquid inlet channel, 4 is the culture chamber, 5 is the outlet, 6 is the liquid outlet channel, 7 is the biomimetic scaffold, 8 is the hollow fiber tube port, 9 is the hollow fiber tube channel, 10, 101, 102, and 103 are hollow fiber tubes, 11 is the gas channel, 12, 121, 122, and 123 are microfluidic chips, 13 is the component unit, 14 is the inlet unit, 15 is the track, and 16, 161, 162, and 163 are gas regulating devices. Detailed Implementation
[0038] The present application is further illustrated below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present application and are not intended to limit the present application.
[0039] The embodiments of this application will be further described in detail below with reference to the accompanying drawings and examples. The detailed description and drawings of the following embodiments are used to illustrate the principles of this application by way of example, but should not be used to limit the scope of this application, that is, this application is not limited to the described embodiments. In the description of this application, it should be noted that, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," etc., indicating orientation or positional relationship are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. In addition, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0040] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this application depending on the specific circumstances.
[0041] like Figures 1 to 6 As shown, this application provides a biodegradable bioscaffold-assisted three-dimensional embryo culture system, characterized in that the culture system comprises:
[0042] Culture substrate 1, microfluidic chip 12, hollow fiber tube 10, gas conditioning device 16;
[0043] The culture body 1 includes: an inlet 2, an outlet 5, a culture chamber 4, a liquid inlet channel 3, and a liquid outlet channel 6;
[0044] The microfluidic chip 12, the hollow fiber tube 10, and the culture body 1 are connected in sequence in the liquid flow direction, and the gas regulating device 16 is connected to the hollow fiber tube 10.
[0045] In one embodiment of this application, in the culture body 1, the inlet 2 is connected to one end of the liquid inlet channel 3, the other end of the liquid inlet channel 3 is connected to the culture chamber 4, the culture chamber 4 is also connected to one end of the liquid outlet channel 6, and the other end of the liquid outlet channel 6 is connected to the outlet 5.
[0046] from Figure 1As can be seen, in one embodiment, the culture body 1 may have two or more culture chambers 4. It should be understood that the nutrient solution flows into the interior of the culture body 1 from the inlet 2 and flows in the inlet channel 3. The inlet channel 3 may have a diversion function (diverting flow through sub-channels). When there are two or more culture chambers 4, the nutrient solution will enter the two or more culture chambers 4 evenly through the diversion effect of the inlet channel 3. When there is sufficient nutrient solution in the culture chamber 4, the nutrient solution will be discharged through the outlet channel 6 and out of the outlet 5. It should be understood that the outlet channel 6 may have a confluence function (corresponding to the diversion function of the inlet channel 3), so when there are multiple culture chambers 4, the culture solution in each culture chamber 4 can be collected at the outlet 5 through the outlet channel 6. The arrangement of multiple culture chambers 4 helps to culture more cells simultaneously and improves culture efficiency.
[0047] It should also be understood that the culture subject 1 may have only one culture chamber, in which case the liquid inlet channel 3 and / or liquid outlet channel 6 may not have the function of diversion and / or confluence.
[0048] exist Figure 2 As can be seen, culture chamber 4 is a chamber with a certain depth. The inlet channel 3 is located below the culture body 1 (in other words, at a deeper level), and connects to the bottom of culture chamber 4 (in other words, at a deeper level). The outlet channel 6 is located above the culture body 1 (in other words, at a shallower level), and connects to the upper part of culture chamber 4 (in other words, at a shallower level). Therefore, the nutrient solution will gradually fill culture chamber 4 from bottom to top. When there is too much nutrient solution, it can flow out through the outlet channel 6, at which point culture chamber 4 will still contain a certain volume of nutrient solution.
[0049] like Figure 2 As shown, a biomimetic scaffold 7 is placed in the culture chamber 4. The volume of the culture chamber 4 is larger than the volume occupied by the biomimetic scaffold 7, allowing the biomimetic scaffold 7 to be completely placed inside. Specifically, when the biomimetic scaffold 7 is in the culture chamber 4, a certain volume of nutrient solution described above can completely submerge the biomimetic scaffold 7. In other words, the height of the outlet channel 6 in the depth direction of the culture chamber 4 can be higher than the height of the biomimetic scaffold 7. In one embodiment of this application, the height of the outlet channel 6 in the depth direction of the culture chamber 4 is higher than the height of the cells on the biomimetic scaffold 7. In one embodiment of this application, the culture body 1 also includes a sealing cap, which is adapted to the culture body 1, specifically to the culture chamber 4. This sealing cap can completely seal the culture chamber 4, preventing the nutrient solution from overflowing while isolating external air.
[0050] In one embodiment of this application, the bionic scaffold 7 is a scaffold with a porous structure, which can have various shapes, such as, but not limited to, fence-shaped, cubic, cuboid, cylindrical, spherical, fibrous mesh, sheet-like, or frustum-shaped, trapezoidal, or customized shapes, such as the shape that simulates the uterus or related anatomical structures (such as fallopian tubes).
[0051] In one embodiment of this application, the cells to be cultured are located at the center of the biomimetic scaffold 7. For example, if the biomimetic scaffold 7 is cubic in shape, the cells to be cultured are preferably located at its geometric center. The purpose of this arrangement is that the cells to be cultured are completely enveloped by the nutrient solution throughout the entire culture process, and during the process of the cells to be cultured into embryos, they will not come into contact with the culture chamber 4 from any direction, which is beneficial to cell growth during the culture process.
[0052] In one embodiment, the biomimetic scaffold 7 minimizes the contact area between the cells to be cultured and the scaffold 7, allowing the cells to be cultured stably on (or within) the scaffold 7 without easily changing their position. Simultaneously, the surface of the cells to be cultured allows for maximum contact with the nutrient solution within the culture chamber 4, promoting better cell growth. For example, the biomimetic scaffold 7 can be a mesh structure, and the cells to be cultured can be situated within the "grid" of the mesh structure.
[0053] The biomimetic scaffold 7 has a porosity greater than 90%, for example, the porosity of the biomimetic scaffold 7 may be 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher. The porosity can be determined by methods well known to those skilled in the art, such as the biomimetic gas adsorption method (BET method). In one embodiment of this application, the biomimetic scaffold 7 is coated with cells to be cultured, such as uterine epithelial cells; the biomimetic scaffold 7 may also be coated with components that promote cell growth and / or development, such as integrin αvβ3.
[0054] In one embodiment of this application, the biomimetic scaffold 7 is fabricated using 3D printing technology. 3D printing technology can obtain a specific shape for the biomimetic scaffold 7 according to specific needs. To simulate the uterine environment and synchronize cell and embryonic development cycles, the biomimetic scaffold 7 needs to be gradually degraded, for example, through gradient degradation. In this application, the gradient degradation design allows for functional regulation from microscopic molecules to macroscopic materials. In one embodiment, the biomimetic scaffold 7 degrades by 30-70% within 7 days, for example, by 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, and 70% within 7 days. In another embodiment, the biomimetic scaffold 7 degrades by 50% within 7 days, and the biomimetic scaffold is completely degraded within 14 days. In one embodiment, the raw material for the 3D-printed biomimetic scaffold 7 is a PLGA / collagen complex, which on the one hand meets the degradation requirements, and on the other hand, collagen can provide nutrients for the cultured cells on the biomimetic scaffold 7. Those skilled in the art can flexibly adjust the material selection and material ratio of the biomimetic scaffold 7 according to actual needs (such as the degradation rate of the scaffold, the nutrients required for cell growth, the specific shape of the scaffold, etc.).
[0055] Figure 3 This is an exemplary schematic diagram of the hollow fiber tube 10 of this application, where 8 is the hollow fiber tube port, 9 is the hollow fiber tube channel, and 11 is the gas channel. It should be understood that a hollow fiber tube 10 may contain multiple hollow fiber tube channels 9. Figure 3 (Only one example is shown in this application). In this application, the nutrient solution enters the hollow fiber tube 10 through the hollow fiber tube port 8 and is evenly distributed to each hollow fiber tube channel 9. Multiple holes exist in the hollow fiber tube channel 9. Figure 3 (As shown in the diagram), the pore allows only gas molecules to pass through but not molecules in the nutrient solution. The hollow fiber tube 10 may also include a corresponding nested support so that the hollow fiber tube 10 is secured in a desired manner.
[0056] Gas channel 11 can be connected to gas regulating device 16, which is used to deliver gas of specific components and concentrations to hollow fiber tube 10. The gas diffuses sufficiently inside hollow fiber tube 10 and maintains a certain concentration, then enters the nutrient solution in hollow fiber tube channel 9 through the holes on hollow fiber tube channel 9 and flows with the nutrient solution. Excess gas is discharged through gas channel 11. Therefore, through gas regulating device 16 and hollow fiber tube 10, the nutrient solution passing through the hollow fiber tube can be made to carry a desired concentration of gas, such as oxygen or carbon dioxide. For example, in one embodiment, the nutrient solution flowing out of the hollow fiber tube includes 5% oxygen to simulate the hypoxic environment in the uterus; in another embodiment, the nutrient solution flowing out of the hollow fiber tube also includes 5% carbon dioxide. It should be understood that port 8 at one end of hollow fiber tube 10 is connected to the culture body 1, specifically, to the inlet 2 of the culture body 1.
[0057] Figure 4 This is a schematic diagram of the microfluidic chip 12 of this application, where 13 represents a component unit. It should be understood that the nutrient solution includes multiple components; therefore, multiple component units 13 can be provided, each component unit 13 capable of containing one or more components of the nutrient solution. Multiple components converge into the inflow unit 14 along predetermined tracks, and after thorough mixing and equilibration, form the nutrient solution, which then flows out of the microfluidic chip 12 through track 15. In one embodiment, the components of the nutrient solution include EGF (epidermal growth factor), VEGF (vascular endothelial growth factor), etc. The number of component units 13 can be determined by those skilled in the art.
[0058] In some nutrient solutions, interactions may exist between components, thus requiring control over the timing of different component additions or the amount of each component in the nutrient solution. Therefore, component unit 13 can be connected to control modules such as microfluidic pumps or syringes. Track 15 is connected to hollow fiber tube 10, specifically, track 15 is connected to hollow fiber tube port 8 (different from port 8 connected to culture body 1).
[0059] In one embodiment of this application, the culture system may further include a heating device disposed between the microfluidic chip 12 and the hollow fiber tube 10, for fully preheating or heating the nutrient solution. In another embodiment of this application, the heating device may also be disposed between the hollow fiber tube 10 and the culture body 1. The purpose of providing the heating device is to simulate human body temperature, ensuring that the nutrient solution is used to culture cells at a suitable temperature. It should be understood that due to the presence of the heating device, temperature changes in the nutrient solution may affect certain components or gas concentrations. Those skilled in the art can adjust accordingly to ensure that the nutrient solution entering the culture chamber is within a suitable temperature range, such as 36.1-37.5°C, while simultaneously ensuring that the concentration or content of each component in the nutrient solution is sufficient for cell culture.
[0060] In one embodiment of this application, the culture system may further include a rotating suspension culture system connected to the culture body 1. More preferably, the rotating suspension culture system is rigidly connected to the culture body 1, or the culture body 1 is placed (or fixedly placed) on the rotating suspension culture system. This arrangement allows the culture body 1 to vibrate or shake according to the frequency and amplitude of the rotating suspension culture system, simulating the Brownian motion of the fallopian tube. In one embodiment of this application, the rotating suspension culture system is a magnetic levitation platform or a six-axis magnetic levitation platform. In one implementation, the precision of the rotating suspension culture system is 2 μm, meaning that the rotating suspension culture system can vibrate or shake with an amplitude not exceeding 2 μm.
[0061] The exemplary usage of the culture system provided in this application will now be described in detail with reference to the accompanying drawings.
[0062] Figure 5 This is a schematic diagram of one embodiment of the culture system of this application. The microfluidic chip 12, hollow fiber tube 10, and culture body 1 are sequentially connected, and the gas regulating device 16 is connected to the hollow fiber tube 10. The various components of the nutrient solution are injected into the component unit 13 using a syringe or microfluidic pump, and then thoroughly mixed in the inlet unit 14 to form the nutrient solution. The nutrient solution flows into the hollow fiber tube 10 through the track 15 and the hollow fiber tube port 8.
[0063] The gas regulating device 16 is connected to the gas channel 11 of the hollow fiber tube 10. Carbon dioxide and oxygen can enter and fill the interior of the hollow fiber tube 10 through the gas regulating device 16 and the gas channel 11, and enter and dissolve into the nutrient solution through the holes in the hollow fiber tube channel 9. If necessary, both the nutrient solution and the gas can remain in the hollow fiber tube 10 for a sufficient time to ensure that the nutrient solution has a sufficient concentration of gas.
[0064] A biomimetic scaffold 7 coated with uterine epithelial cells and integrin αvβ3 is placed in the culture chamber beforehand, and a sealing cap is installed. A nutrient solution containing gas flows out from another port 8 of the hollow fiber tube 10, enters the culture body 1 through inlet 2, and then enters and fills the culture chamber 4 via the inlet channel 3, completely submerging the biomimetic scaffold 7 in the culture chamber 4. The biomimetic scaffold 7 undergoes gradient degradation in the nutrient solution, synchronizing with the cell development cycle, allowing the cells to enter the next stage of embryo culture. Waste liquid generated during culture can flow out from outlet 5 through outlet channel 6. In one embodiment of this application, the nutrient solution can be assisted in flowing or remaining in the culture system by installing a suction pump at outlet 5 or by using a microfluidic pump at component unit 13.
[0065] In another embodiment of this application, such as in Figure 6 In this configuration, multiple microfluidic chips (121, 122, 123) can be connected in parallel, along with corresponding hollow fiber tubes (101, 102, 103) and gas regulating devices (161, 162, 163). Each hollow fiber tube is connected to one of the microfluidic chips, and all hollow fiber tubes are connected to the culture medium 1. In one embodiment, the culture medium 1 may also have multiple inlets 2, the number of inlets equal to the number of hollow fiber tubes, to connect to different hollow fiber tube (101, 102, 103) ports 8.
[0066] As is well known, the growth and development of oocytes can be simply divided into three stages: the maturation IVM stage, the fertilization IVF stage, and the culture IVC stage. Different culture media are required for different culture stages. Therefore, a biomimetic scaffold 7 coated with uterine epithelial cells can be placed in the culture chamber 4 beforehand and sealed. During the oocyte maturation IVM stage, only three devices—microfluidic chip 121, hollow fiber tube 101, and gas regulator 161—are activated, and embryo culture is carried out as described above. After the oocyte maturation IVM stage is completed, the above three devices are turned off, and the microfluidic chip 122, hollow fiber tube 102, and gas regulator 162 are activated. At this time, the nutrient solution from the microfluidic chip 121 in the culture chamber 4 becomes waste liquid and is discharged from the outlet 5. The nutrient solution from the microfluidic chip 122 enters the culture chamber 4, and the oocyte fertilization IVF stage begins. This process continues until those skilled in the art consider the embryo culture complete.
[0067] It should be understood that those skilled in the art can adjust the culture system by distinguishing the culture stage or culture status of the cells. Adjustments include, but are not limited to, the components of the nutrient solution in the microfluidic chip (even in the IVF stage, the components can be sperm cells), the structure of the hollow fiber tube, the composition and type of gas provided in the gas regulating device, and the desired degradation time of the biomimetic scaffold. In one embodiment, the culture system can also be connected to a computer program, automated equipment, circuit boards, etc., for example, to regulate the input and output of nutrient solution components, the transformation of culture stages, gas content control, and the setting of culture time, in order to automatically control the culture system of this application.
[0068] The microfluidic chip, hollow fiber tube, gas conditioning device and culture body are independent of each other and are modularly designed. Those skilled in the art can flexibly adjust the specific details of each module as needed. For example, if some nutrient solutions contain only three components, a microfluidic chip 12 with three component units 13 can be selected.
[0069] The culture system provided in this application effectively simulates the three-dimensional environment of the uterus using a biodegradable scaffold, and a gas regulation device is used to simulate the low-oxygen environment inside the uterus, helping cells to develop into blastocysts better and increasing the blastocyst conversion rate. Simultaneously, the modular design allows those skilled in the art to adjust the culture conditions controlled by each module of the culture system according to actual needs. Each module is independent and does not affect the others, thus enabling continuous multi-stage cell culture without the need for additional cell transfer. Therefore, this application offers extremely high flexibility.
[0070] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A biodegradable biological scaffold assisted three-dimensional culture system for bovine embryos, characterized in that, The culture system comprises: a culture main body, a microfluidic chip, a hollow fiber tube, and a gas regulating device; the culture main body comprises an inlet, an outlet, a culture cavity, a liquid inlet channel, and a liquid outlet channel; the microfluidic chip, the hollow fiber tube, and the culture main body are sequentially connected in the liquid flow direction, and the gas regulating device is connected to the hollow fiber tube.
2. The culture system according to claim 1, wherein in the culture main body, the inlet is connected to one end of the liquid inlet channel, the other end of the liquid inlet channel is connected to the culture cavity, the culture cavity is further connected to one end of the liquid outlet channel, and the other end of the liquid outlet channel is connected to the outlet.
3. The culture system according to claim 1, wherein a biomimetic scaffold is placed in the culture cavity, and the volume of the culture cavity is greater than the volume occupied by the biomimetic scaffold.
4. The culture system according to claim 3, wherein the biomimetic scaffold has a porous structure, and the cells to be cultured are located at the center of the biomimetic scaffold, and the porosity of the biomimetic scaffold is greater than 90%.
5. The culture system according to claim 1, wherein in the direction of the depth of the culture cavity, the liquid inlet channel is located below the liquid outlet channel.
6. The culture system according to claim 1, wherein the culture main body is further provided with a sealing cover, and the sealing cover is adapted to the culture cavity.
7. The culture system according to claim 1, wherein the hollow fiber tube comprises a hollow fiber tube channel and a gas channel, and the hollow fiber tube channel has a hole.
8. The culture system according to claim 1, wherein the culture system comprises a plurality of hollow fiber tubes and a plurality of microfluidic chips, the plurality of hollow fiber tubes are respectively connected to the plurality of microfluidic chips, and the plurality of hollow fiber tubes are all connected to the culture main body.
9. The culture system according to claim 8, wherein the culture main body has a plurality of inlets, and the number of the inlets is equal to the number of the hollow fiber tubes.
10. The culture system according to claim 1, wherein the culture system is connected to a computer program, an automatic device, or a circuit board.