Personalized assembly freezing tank for freezing embedding instrument

Abstract

The utility model belongs to the technical field of freezing embedding instruments, and discloses a personalized assembly freezing tank for a freezing embedding instrument, which comprises a tank body, a sample frame is arranged in the tank body, the sample frame is connected with a push-out component, the push-out component is mounted on the tank body, a positioning component is mounted on the sample frame, a tank cover is arranged on the tank body, and the sample frame is connected with a positioning component. The groove cover is connected with the opening and closing driving assembly, the opening and closing driving assembly is arranged on the groove body and drives the sample frame to leave the inner cavity of the groove body, namely, the sample tissue is pushed out of the freezing groove, workers do not need to stretch hands into the freezing groove to take and place the sample tissue, frostbite caused by the fact that the hands stretch into the freezing groove can be effectively avoided, the workers are protected, and the working efficiency is improved. Each layer of tissue sample is positioned and fixed through the positioning assembly after being placed, so that other tissue samples are prevented from falling and being damaged accidentally when the tissue samples are taken and placed, and unnecessary loss is avoided.

Description

Technical Field

[0001] This utility model belongs to the field of cryo-embedding technology, specifically a cryo-embedding tank for personalized assembly of cryo-embedding instruments. Background Technology

[0002] A cryoembedder is an instrument used for the cryoembedding of biological samples, primarily used in fields such as medicine and biology. Its main function is to rapidly freeze biological tissue or cell samples and embed them at low temperatures in a specific medium, such as paraffin, resin, or gelatin. This allows the sample to retain its original morphology, structure, and biological activity, facilitating subsequent analysis such as sectioning, staining, and microscopic observation. The refrigeration system rapidly lowers the temperature of the sample chamber to the required low temperature, typically between -20°C and -80°C, rapidly freezing the biological sample. Simultaneously, utilizing the solidification properties of the embedding medium at low temperatures, the sample is encapsulated within it, forming a robust embedding block.

[0003] The cryo-embedding chamber is a key component of the cryo-embedding system. It is mainly used to contain and freeze the embedding agent and tissue samples. The main function of the cryo-embedding chamber is to lower the internal temperature to the required low temperature range through the refrigeration system to meet the cryo-embedding needs of different tissue samples. It can quickly freeze tissue samples to low temperature, which can prevent tissue deformation and degradation during the embedding process and maintain the morphology and structural integrity of the tissue.

[0004] In existing technologies, when taking or taking tissue samples from a cryostat, staff need to put their hands inside. Since cryostats are usually at low temperatures, this poses certain safety hazards during the process. Furthermore, if multiple tissue samples are to be frozen simultaneously, the samples need to be placed on a sample rack. If the placed samples are not properly positioned, other samples may be accidentally bumped during handling, leading to accidental drops and damage.

[0005] Therefore, a cryogenic bath for personalized assembly of cryoembedding instruments is proposed to address the above problems. Utility Model Content

[0006] To address the problems mentioned in the background art, this utility model provides a personalized assembly freezing tank for cryoembedding instruments, which has the advantage of pushing out tissue samples from the tank and avoiding frostbite caused by workers putting their hands into the tank.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a cryoembedding instrument for personalized assembly cryotherapy, comprising a tank body, a sample rack disposed within the tank body, a push-out component connected to the sample rack, the push-out component mounted on the tank body, a positioning component mounted on the sample rack, a tank cover disposed on the tank body, and an opening and closing drive component connected to the tank cover, the opening and closing drive component being disposed on the tank body.

[0008] Preferably, the ejection assembly includes a lead screw and a positioning slide rod, the bottom end of the lead screw is connected to a first motor, a lifting block is connected between the lead screw and the positioning slide rod, the lead screw is threaded to the lifting block, the positioning slide rod passes through the lifting block, and the lifting block is fixedly connected to the sample holder.

[0009] Preferably, the positioning component includes two sets of clamping members, each clamping member being movably connected to a guide rod and a screw. The guide rod is slidably connected to the clamping member, and the screw is threadedly connected to the clamping member. Fixed blocks are fixedly connected to both ends of the guide rod, and one end of the screw is connected to the corresponding fixed block via a bearing, while the other end passes through the corresponding fixed block and is connected to a knob.

[0010] Preferably, each set of clamping components consists of two symmetrically arranged arc-shaped rods, and the two arc-shaped rods in the same set move in opposite directions.

[0011] Preferably, the arc-shaped rod is provided with guide holes and threaded holes corresponding to the guide rod and the screw, respectively.

[0012] Preferably, the lifting block is provided with a positioning sliding hole and a threaded groove, the positioning sliding rod is disposed in the positioning sliding hole, and the threaded groove is threadedly connected to the lead screw.

[0013] Preferably, the opening and closing drive assembly includes a U-shaped frame that is fixedly connected to the slot cover. The U-shaped frame is connected to a diagonal brace via a connecting shaft. A first rotating shaft is installed at the bottom of the U-shaped frame. The first rotating shaft is connected to an electric telescopic rod. The electric telescopic rod is connected to a second rotating shaft, which is located on the slot body.

[0014] Preferably, a stirring assembly is provided on both sides of the tank. The stirring assembly includes a rotating shaft disposed in the tank, and uniformly distributed stirring blades are disposed on the rotating shaft. The rotating shaft is connected to a second motor through a motor shaft.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] 1. This utility model, by setting up an ejection component, drives the sample holder to leave the inner cavity of the freezing tank, that is, to eject the sample tissue from the freezing tank. This eliminates the need for staff to put their hands into the freezing tank to pick up or put in the sample tissue, effectively avoiding frostbite caused by putting their hands into the freezing tank, thus protecting the staff.

[0017] 2. By setting up a positioning component, this utility model allows multiple tissue samples to be placed on a sample rack when freezing. After each layer of tissue samples is placed, it is positioned and fixed by the positioning component to avoid accidental drop and damage to other tissue samples when taking or taking out tissue samples, thus avoiding unnecessary losses. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a structural schematic diagram of the present invention from another angle;

[0020] Figure 3 This is a schematic diagram of the opening and closing drive assembly of this utility model;

[0021] Figure 4 This is a schematic diagram of the internal structure of the tank body of this utility model;

[0022] Figure 5 This is a schematic diagram of the sample holder of this utility model;

[0023] Figure 6 This is a structural schematic diagram of the component proposed in this utility model;

[0024] Figure 7 This is a schematic diagram of the positioning component of this utility model;

[0025] Figure 8 This is a schematic diagram of the structure of the stirring assembly of this utility model.

[0026] In the diagram: 1. Tank; 2. Sample rack;

[0027] 3. Ejection components; 301. Lead screw; 302. Positioning slide bar; 303. First motor; 304. Lifting block;

[0028] 4. Positioning assembly; 401. Clamping component; 4011. Arc-shaped rod; 402. Guide rod; 403. Screw; 404. Fixing block; 405. Knob;

[0029] 5. Slot cover;

[0030] 6. Opening and closing drive assembly; 601. U-shaped frame; 602. Connecting shaft; 603. Diagonal brace; 604. First shaft; 605. Electric telescopic rod; 606. Second shaft;

[0031] 7. Stirring assembly; 701. Rotating shaft; 702. Stirring blades; 703. Second motor. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] like Figures 1 to 8 As shown, this utility model provides a cryogenic bath for personalized assembly of cryogenic embedding instruments, including a bath body 1. The bath body 1 is usually made of materials with good low-temperature resistance and corrosion resistance, such as stainless steel or aluminum alloy. The cryogenic bath has a dedicated position for installing components such as the evaporator and temperature sensor of the refrigeration system. Its shape and size are determined according to different cryogenic embedding instrument models and usage requirements, and are generally cuboid or cylindrical. In order to reduce heat loss and maintain the low-temperature environment inside the cryogenic bath, the outside of the bath body 1 is usually wrapped with a layer of insulation material, such as polyurethane foam or polystyrene. A sample rack 2 is set inside the bath body 1. The sample rack 2 is composed of multiple layers of grid plates, and the adjacent grid plates are connected by support rods. The sample rack 2 is connected to a push-out component 3, which is installed on the bath body 1. A positioning component 4 is installed on the sample rack 2. A bath cover 5 is set on the bath body 1, and the bath cover 5 is connected to an opening and closing drive component 6, which is located on the bath body 1.

[0034] Specifically, the ejection component 3 includes a lead screw 301 and a positioning slide bar 302. The bottom end of the lead screw 301 is connected to a first motor 303. A lifting block 304 is connected between the lead screw 301 and the positioning slide bar 302. The lead screw 301 is threadedly connected to the lifting block 304. The positioning slide bar 302 passes through the lifting block 304. The lifting block 304 is fixedly connected to the sample holder 2, driving the sample holder 2 to leave the inner cavity of the tank 1, that is, to eject the sample tissue from the freezing tank. This eliminates the need for staff to put their hands into the freezing tank to pick up or put down the sample tissue, effectively preventing frostbite caused by putting their hands into the freezing tank, thus protecting the staff.

[0035] Furthermore, the positioning component 4 includes two sets of clamping members 401. The clamping members 401 are movably connected to a guide rod 402 and a screw 403, respectively. The guide rod 402 is slidably connected to the clamping member 401, and the screw 403 is threadedly connected to the clamping member 401. The two ends of the guide rod 402 are fixedly connected to fixing blocks 404. One end of the screw 403 is connected to the corresponding fixing block 404 through a bearing, and the other end passes through the corresponding fixing block 404 and is connected to a knob 405. When freezing multiple tissue samples, the tissue samples can be placed on the sample rack 2. After each layer of tissue samples is placed, it is positioned and fixed by the positioning component 4 to avoid accidental drop and damage to other tissue samples when picking up or placing tissue samples, thus avoiding unnecessary losses.

[0036] Furthermore, each set of clamping components 401 consists of two symmetrically arranged arc-shaped rods 4011. The two arc-shaped rods 4011 in the same set move in opposite directions, so that the two arc-shaped rods 4011 can clamp and fix tissue samples.

[0037] It is worth noting that the arc-shaped rod 4011 is provided with guide holes and threaded holes corresponding to the guide rod 402 and the screw 403, and the displacement of the arc-shaped rod 4011 is driven by the threaded structure and the guide structure.

[0038] It is worth noting that the lifting block 304 is provided with a positioning sliding hole and a threaded groove, and the positioning sliding rod 302 is located in the positioning sliding hole. The threaded groove is threadedly connected to the lead screw 301, and the structure of the lead screw 301 can drive the lifting block 304 to move.

[0039] It is worth mentioning that the opening and closing drive assembly 6 includes a U-shaped frame 601 that is fixedly connected to the slot cover 5. The U-shaped frame 601 is connected to a diagonal support frame 603 via a connecting shaft 602. A first shaft 604 is installed at the bottom of the U-shaped frame 601. The first shaft 604 is connected to an electric telescopic rod 605. The electric telescopic rod 605 is connected to a second shaft 606. The second shaft 606 is located on the slot body 1. The automatic opening and closing of the slot cover 5 can reduce the time for operators to manually open and close the door, while improving the convenience and automation of operation.

[0040] It is worth emphasizing that stirring components 7 are provided on both sides of the tank 1. The stirring components 7 include a rotating shaft 701 located inside the tank 1, and uniformly distributed stirring blades 702 on the rotating shaft 701. The rotating shaft 701 is connected to a second motor 703 through a motor shaft, so that the refrigerant (such as air or coolant) circulates in the tank 1, avoiding temperature stratification and ensuring temperature uniformity.

[0041] The first motor 303, the electric telescopic rod 605, and the second motor 703 are existing technologies and will not be described in detail. In addition, this utility model also includes a power supply, a controller, and a switch, which are not the main technical points of this patent and will not be described in detail.

[0042] Working principle and process: The electric telescopic rod 605 of the opening / closing drive assembly 6 is activated, causing it to retract. Under the action of the first rotating shaft 604 and the second rotating shaft 606, the U-shaped frame 601 rotates around the connecting rotating shaft 602, allowing the tank cover 5 to leave the tank body 1, thus opening the freezing tank. The first motor 303 of the ejection assembly 3 is activated, causing the lead screw 301 to rotate. Under the action of the threaded groove and the positioning slide rod 302, the lifting block 304 moves the sample holder 2 upward, allowing the sample holder 2 to leave the inner cavity of the tank body 1. The tissue sample is then placed on the sample holder 2. The positioning assembly 4 is then rotated. Knob 405 is turned, which in turn rotates screw 403. Under the action of threaded hole and guide rod 402, two arc rods 4011 of the same group move closer to each other, which can clamp and fix tissue sample. Run push component 3 again to let tissue sample enter tank 1 with sample holder 2. Run open and close drive component 6 again to close tank cover 5. During freezing, start second motor 703 of stirring component 7, which in turn rotates shaft 701 to drive stirring blade 702 to rotate, so that freezing medium (such as air or coolant) circulates in tank 1 to avoid temperature stratification and ensure temperature uniformity.

[0043] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0044] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cryomodle for individualized assembly of cryotubes, comprising a tub (1), characterized in that: The sample frame (2) is arranged in the groove body (1), the sample frame (2) is connected with the push-out assembly (3), the push-out assembly (3) is installed on the groove body (1), the sample frame (2) is installed with the positioning assembly (4), the groove cover (5) is arranged on the groove body (1), the groove cover (5) is connected with the opening and closing drive assembly (6), and the opening and closing drive assembly (6) is arranged on the groove body (1).

2. A personalized assembly freezing tank for a freezing embedding instrument according to claim 1, characterized in that: The push-out assembly (3) comprises correspondingly arranged lead screws (301) and positioning sliding rods (302), the bottom end of the lead screw (301) is connected with a first motor (303), the lead screw (301) and the positioning sliding rod (302) are connected with a lifting block (304), the lead screw (301) is screwed with the lifting block (304), the positioning sliding rod (302) penetrates the lifting block (304), and the lifting block (304) is fixedly connected with the sample frame (2).

3. A personalized assembly freezing tank for a freezing embedding instrument according to claim 1, characterized in that: The positioning assembly (4) comprises two groups of clamping pieces (401) arranged correspondingly, the clamping piece (401) is movably connected with a guide rod (402) and a screw rod (403) respectively, the guide rod (402) is slidably connected with the clamping piece (401), the screw rod (403) is screwed with the clamping piece (401), both ends of the guide rod (402) are fixedly connected with a fixed block (404), one end of the screw rod (403) is connected with the corresponding fixed block (404) through a bearing, and the other end penetrates the corresponding fixed block (404) and is connected with a knob (405).

4. A personalized assembly freezing tank for a freezing embedding instrument according to claim 3, characterized in that: Each group of clamping pieces (401) is composed of two symmetrically arranged arc-shaped rods (4011), and the moving directions of the two arc-shaped rods (4011) of the same group are opposite.

5. A personalized assembly freezing tank for a freezing embedding instrument according to claim 4, characterized in that: Corresponding guide holes and threaded holes are formed in the arc-shaped rods (4011) respectively, and the guide holes and the threaded holes are correspondingly arranged with the guide rods (402) and the screw rods (403).

6. A personalized assembly freezing tank for a freezing embedding instrument according to claim 2, characterized in that: A positioning sliding hole and a threaded groove are formed in the lifting block (304) respectively, the positioning sliding rod (302) is arranged in the positioning sliding hole, and the threaded groove is screwed with the lead screw (301).

7. A personalized assembly freezing tank for a freezing embedding instrument according to claim 1, characterized in that: The opening and closing drive assembly (6) comprises a U-shaped frame (601) fixedly connected with the groove cover (5), the U-shaped frame (601) is connected with an inclined support frame (603) through a connecting shaft (602), a first rotating shaft (604) is arranged at the bottom of the U-shaped frame (601), the first rotating shaft (604) is connected with an electric telescopic rod (605), the electric telescopic rod (605) is connected with a second rotating shaft (606), and the second rotating shaft (606) is arranged on the groove body (1).

8. A personalized assembly freezing tank for a freezing embedding instrument according to claim 1, characterized in that: Both sides of the groove body (1) are provided with stirring assemblies (7), the stirring assembly (7) comprises a rotating shaft (701) arranged in the groove body (1), the rotating shaft (701) is provided with uniformly distributed stirring paddles (702), and the rotating shaft (701) is connected with a second motor (703) through a motor shaft.

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