Disturbance device for adherent culture of microalgae

Abstract

The utility model relates to the technical field of microalgae culture, in particular to a disturbance device for adherent culture of microalgae, which comprises a support and a culture device, the culture device is mounted on the support, a disturbance mechanism is arranged at the top of the support and comprises a motor, the motor is arranged above the culture device, and the disturbance mechanism is arranged on the support. An output shaft of the motor is located at the bottom of the motor, an outer magnetic rotor is fixedly connected to the output shaft of the motor, an inner magnetic rotor is arranged in the culture device, the inner magnetic rotor is coaxially arranged below the outer magnetic rotor, and the adjacent surfaces of the outer magnetic rotor and the inner magnetic rotor are like magnetic poles. According to the device, non-contact power transmission is realized, mechanical friction is thoroughly eliminated, a low-shear-force vortex is generated by utilizing a fluid dynamic pressure effect to strip an over-thick aged algae layer, the damage rate of a biological membrane is reduced, the universality of equipment is improved, and reliable technical support is provided for high-density adherent culture of microalgae.

Description

Technical Field

[0001] This utility model relates to the field of microalgae cultivation technology, specifically to a disturbance device for microalgae adherent culture. Background Technology

[0002] As a core process for the production of bioenergy and high-value products, the key challenge of microalgae adherent culture technology lies in how to achieve efficient disturbance and renewal of the biofilm while avoiding damage to microalgae cells by mechanical or fluid shear forces.

[0003] In the existing technology, although various disturbance devices have been proposed, their design ideas are mostly limited to direct contact mechanical scraping or high-energy-consuming fluid drive, resulting in significant defects in practical applications. A published patent for a Haematococcus pluvialis culture device, publication number CN221836978U, publication date: January 2024, uses air pump-driven mechanical stirring blades to mix the culture medium by rotating and adjusting the container direction. Although it improves the mixing uniformity to a certain extent, the rigid blades directly contact the algal layer, generating strong shear force under high-speed rotation. The measured cell survival rate is insufficient, and the multi-motor linkage structure leads to high system energy consumption, making it difficult to meet the low-cost requirements of industrial production and avoid damage to microalgal cells by mechanical or fluid shear force. Utility Model Content

[0004] To address the aforementioned technical problems or one of the technical problems existing in the prior art, this utility model discloses a disturbance device for adherent microalgae cultivation, comprising a support and a cultivation device. The cultivation device is mounted on the support, and a disturbance mechanism is provided at the top of the support. The disturbance mechanism includes a motor, which is located above the cultivation device. The output shaft of the motor is located at the bottom of the motor, and an outer magnetic rotor is fixedly connected to the output shaft of the motor. An inner magnetic rotor is provided inside the cultivation device, and the inner magnetic rotor is coaxially arranged downwards from the outer magnetic rotor. The adjacent surfaces of the outer magnetic rotor and the inner magnetic rotor are of the same magnetic poles and are driven by magnetic repulsion coupling. A connecting shaft is provided at the bottom of the inner magnetic rotor, and a silicone sleeve is fitted at the bottom of the connecting shaft. A disturbance plate is fixedly connected to the outside of the silicone sleeve.

[0005] Furthermore, the bracket includes a base, a main support rod, a secondary support rod, a support beam, and a fixing ring. The top surface of the base has a groove, and the main support rod is located on one side of the groove. The main support rod has a tubular structure with multiple pin holes arranged vertically on its side. The bottom end of the secondary support rod is located inside the main support rod, and it also has multiple pin holes arranged vertically on its side. Each pin hole contains a pin. The main support rod and the secondary support rod are fixed together by the pins. One end of the support beam is fixedly connected to the top end of the secondary support rod, and the other end is fixedly connected to the fixing ring. The motor is fixedly connected inside the fixing ring.

[0006] Furthermore, the culture device includes a culture dish, a sealing cap, and a guide rail. The sealing cap is installed at the top opening of the culture dish, and the guide rail is annular and fixed to the inner wall of the culture dish.

[0007] Furthermore, the culture dish is placed in the groove.

[0008] Furthermore, a support arm is fixedly connected to the outer side of the inner magnetic rotor, a support ring is fixedly connected to the outer end of the support arm, a support arm is fixedly connected to the outer side of the support ring, and a spherical slider is fixedly connected to the outer end of the support arm. The slider is disposed on the guide rail.

[0009] Furthermore, the disturbance sheet is a wavy silicone sheet with an edge at a 45° angle.

[0010] Furthermore, the surface of the guide rail is coated with a polytetrafluoroethylene layer.

[0011] Compared with existing technologies, this utility model aims to systematically solve existing problems through a triple innovative design: First, it adopts a Halbach array to optimize the magnetic pole arrangement, and achieves contactless power transmission through the axial isotropic repulsive force between the outer and inner magnetic rotors, completely eliminating mechanical friction. Combined with the 45° inclination angle design of the wavy silicone disturbance sheet, it utilizes the hydrodynamic pressure effect to generate low-shear eddy currents to peel off excessively thick aging algal layers, reducing the biofilm damage rate. Second, through a modular support and pin adjustment mechanism, it adapts to containers of different heights and diameters. Combined with the detachable culture dish and groove quick-change design, it enables switching between flat, cylindrical, and flexible bag reactors within 5 minutes, significantly improving the equipment's versatility. Finally, relying on the precise cooperation of the PTFE-coated guide rail and the spherical slider, combined with the rigid reinforcement structure of the truss support arm, it ensures the concentric rotation accuracy of the inner magnetic rotor, providing reliable technical support for high-density adherent microalgae culture. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0013] Figure 2 This is a schematic diagram of the cross-sectional structure of the present invention;

[0014] Figure 3 This is a schematic diagram of the internal magnetic rotor structure of this utility model. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0016] Please see Figure 1-3 The technical solution adopted by this utility model is as follows: a disturbance device for microalgae adherent culture, comprising a support 1 and a culture device 2. The culture device 2 is mounted on the support 1. A disturbance mechanism is provided on the top of the support 1. The disturbance mechanism includes a motor 3, which is located above the culture device 2. The output shaft of the motor 3 is located at the bottom of the motor 3. An outer magnetic rotor 41 is fixedly connected to the output shaft of the motor 3. An inner magnetic rotor 42 is provided inside the culture device 2. The inner magnetic rotor 42 is coaxially arranged below the outer magnetic rotor 41. The adjacent surfaces of the outer magnetic rotor 41 and the inner magnetic rotor 42 are of the same magnetic poles NN or SS. A Halbach array is used at the contact surface of the outer magnetic rotor and the inner magnetic rotor. The N-pole-down outer magnetic rotor and the N-pole-up inner magnetic rotor form a repulsive force, which is driven by magnetic repulsive coupling. A connecting shaft 5 is provided at the bottom of the inner magnetic rotor 42. A silicone sleeve 6 is fitted at the bottom of the connecting shaft 5. A disturbance piece 61 is fixedly connected to the outside of the silicone sleeve 6.

[0017] Preferably, the support 1 includes a base 11, a main support rod 12, a secondary support rod 14, a support beam 15, and a fixing ring 16. The top surface of the base 11 has a groove 17. The main support rod 12 is located on one side of the groove 17 to fix the culture dish 21, ensuring its horizontal placement and stable position, preventing the container from sliding or tilting during cultivation. The main support rod 12 has a tubular structure with multiple pin holes arranged vertically on its side. The bottom end of the secondary support rod 14 is located inside the main support rod 12, and its side also has multiple pin holes arranged vertically. The support has a pin 13 inside, and the main support rod 12 and the secondary support rod 14 are fixed by the pin. The vertical array design of the pin holes and pins 13 enables multi-level adjustment of the support height to adapt to different sizes of cultivation devices. One end of the support beam 15 is fixed to the top of the secondary support rod 14, and the other end is fixed to the fixing ring 16. The motor 3 is fixed inside the fixing ring 16. The support beam connects the secondary support rod and the fixing ring to form a rigid frame. The fixing ring is used to accurately install the motor 3 to ensure that the outer magnetic rotor 41 and the inner magnetic rotor 42 are coaxially aligned.

[0018] Preferably, the culture device 2 includes a culture dish 21, a sealing cap 22, and a guide rail 23. The sealing cap 22 is installed at the top opening of the culture dish 21 to provide a closed microalgae adhering culture environment. The inner wall is smooth to promote uniform biofilm adhesion. A silicone sealing ring is used to prevent evaporation of the culture medium and external contamination, maintaining a sterile environment. The guide rail 23 is annular and fixed to the inner wall of the culture dish 21, providing a precise movement track for the spherical slider 46 and ensuring the concentricity of the rotation of the inner magnetic rotor 42.

[0019] Preferably, the culture dish 21 is placed in the groove 17, the inner contour of the groove 17 matches the bottom of the culture dish 21, and can also be fixed by buckles or magnetic attraction to achieve quick positioning and disassembly.

[0020] Preferably, a support arm 43 is fixedly connected to the outer side of the inner magnetic rotor 42, a support ring 44 is fixedly connected to the outer end of the support arm 43, and a support arm 45 is fixedly connected to the outer side of the support ring 44. A lightweight aluminum alloy truss structure is adopted to transmit the rotational torque of the inner magnetic rotor 42 to the guide rail 23, while reducing the inertial torque. A spherical slider 46 is fixedly connected to the outer end of the support arm 45. The slider 46 is disposed on the guide rail 23 and cooperates with the guide rail 23 to reduce friction and energy loss.

[0021] Preferably, the disturbance sheet 61 is a wavy silicone sheet with alternating crests and troughs to generate periodic eddies, enhancing radial mixing of the culture medium and improving nutrient transfer efficiency. The edge is inclined at 45° to optimize the direction of fluid shear force and avoid biofilm tearing. By changing silicone sleeves of different specifications 6, the distance between the disturbance sheet 61 and the inner wall of the culture dish can be adjusted (1-3mm) to achieve non-contact or light contact disturbance.

[0022] Preferably, the surface of the guide rail 23 is coated with a polytetrafluoroethylene layer to reduce the movement resistance of the spherical slider 46, resist the chemical corrosion of the culture medium, and prevent the metal guide rail from rusting and becoming contaminated.

[0023] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for perturbation of microalgae adherent culture, comprising a support (1) and a culture device (2), said culture device (2) being mounted on the support (1), characterized in that, The top of the support (1) is provided with a disturbance mechanism, the disturbance mechanism comprises a motor (3), the motor is arranged above the culture device (2), the output shaft of the motor (3) is located at the bottom of the motor (3), the output shaft of the motor (3) is fixedly connected with an outer magnetic rotor (41), the inside of the culture device (2) is provided with an inner magnetic rotor (42), the inner magnetic rotor (42) is coaxially arranged downwardly in the outer magnetic rotor (41), the adjacent surfaces of the outer magnetic rotor (41) and the inner magnetic rotor (42) are same magnetic poles, and the adjacent surfaces are coupled and driven through magnetic repulsion, the bottom of the inner magnetic rotor (42) is provided with a connecting shaft (5), the bottom of the connecting shaft (5) is sleeved with a silica gel sleeve (6), and the outer side of the silica gel sleeve (6) is fixedly connected with a disturbance sheet (61).

2. The device for perturbation of microalgal adherent culture according to claim 1, wherein, The support (1) comprises a base (11), a main support rod (12), a vice support rod (14), a support beam (15) and a fixing ring (16), the top surface of the base (11) is provided with a groove (17), the main support rod (12) is arranged on one side of the groove (17), the main support rod (12) is in a tubular structure, and a plurality of pin holes are vertically arranged on the side surface, the bottom end of the vice support rod (14) is arranged in the inside of the main support rod (12), a plurality of pin holes are vertically arranged on the side surface, a pin column (13) is arranged in the pin hole, and the main support rod (12) and the vice support rod (14) are fixed through the pin column, one end of the support beam (15) is fixedly connected with the top end of the vice support rod (14), the other end is fixedly connected with the fixing ring (16), and the motor (3) is fixedly connected in the fixing ring (16).

3. The device for perturbation of microalgal adherent culture according to claim 1, wherein, The culture device (2) comprises a culture dish (21), a sealing cover (22) and a guide rail (23), the sealing cover (22) is installed at the opening in the top of the culture dish (21), and the guide rail (23) is annular and is fixedly connected to the inner wall of the culture dish (21).

4. The device for perturbation of a microalgal adherent culture according to claim 3, wherein, The culture dish (21) is placed in the groove (17).

5. The device for perturbation of adherent microalgal culture according to claim 3, wherein, The outer side surface of the inner magnetic rotor (42) is fixedly connected with a support large arm (43), the outer side end of the support large arm (43) is fixedly connected with a support ring (44), the outer side surface of the support ring (44) is fixedly connected with a support small arm (45), the outer side end of the support small arm (45) is fixedly connected with a spherical sliding block (46), and the sliding block (46) is arranged on the guide rail (23).

6. The device for perturbation of adherent microalgal culture according to claim 1, wherein, The disturbance sheet (61) is a wavy silica gel sheet, and the edge is inclined at an angle of 45°.

7. The device for perturbation of adherent microalgal culture according to claim 3, wherein, The surface of the guide rail (23) is coated with a polytetrafluoroethylene layer.

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