Intelligent real-time cell culture observation equipment

Abstract

The utility model discloses intelligent real-time cell culture observation equipment, which relates to the technical field of cell culture and comprises an equipment main body, and observation components are arranged on two sides in the equipment main body; the observation assembly comprises a support and a partition plate, a first motor is installed on one side of the outer surface of the support, the output end of the first motor is connected with a first lead screw, and an adjusting frame is connected between the first lead screw and the support. Through the arrangement of the digital microscope, the artificial intelligence controller and the touch screen, a worker can control the digital microscope through the touch screen, so that the cell culture condition in the culture dish is observed in real time through the digital microscope, the culture dish does not need to be taken out, the operation is simple, convenient and safe, and the working efficiency is improved. The digital microscope can be automatically controlled to work through the artificial intelligence controller, so that the cell culture condition in the culture dish can be automatically observed; real-time observation can be realized without taking out the culture dish, and various parameters of the intelligent control equipment can be automatically observed.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to an intelligent real-time cell culture observation device. Background Technology

[0002] Cell culture refers to the process of taking cells from animals or plants and growing them in a suitable artificial environment. It is one of the main tools used in cell and molecular biology, providing an excellent model system for the study of normal cell physiology and biochemistry, the effects of drugs and toxic compounds on cells, and the study of mutagenicity and carcinogenicity. Cell culture can also be used for drug screening and development, as well as the large-scale production of biological compounds.

[0003] In existing cell culture processes, if we want to observe the growth of cells in order to adjust the amount of nutrients added, as well as adjust various parameters such as temperature, humidity, oxygen content, and carbon dioxide content, and to check whether the cells have died or meet the standards, we need to be able to do so.

[0004] However, observation requires staff to remove the culture dishes containing cells from the culture equipment and then put them back after observation. Firstly, the operation is quite cumbersome, requiring opening and closing the sealed door and taking protective measures. Secondly, when removing and placing the culture dishes containing cells, the outside air will exchange with the air inside the equipment, affecting parameters such as temperature, humidity, oxygen content, and carbon dioxide content. This may cause the cells to become unsuited to the environment and weaken or die, thus affecting the culture effect. Utility Model Content

[0005] Based on this, the purpose of this utility model is to provide an intelligent real-time cell culture observation device to solve the technical problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an intelligent real-time cell culture observation device, comprising a main body, with observation components arranged on both sides inside the main body; the observation components include a support and a partition, and a first motor is installed on one side of the outer surface of the support, the output end of the first motor is connected to a first lead screw, and an adjustment frame is connected between the first lead screw and the support; a second motor is installed on one side of the adjustment frame, the output end of the second motor is connected to a second lead screw, an adjustment block is connected to the bottom of the second lead screw, and a digital microscope is installed at the bottom of the adjustment block; a control component is arranged below one side of the main body, and the control component includes an artificial intelligence controller, with a touch screen mounted at an angle on the artificial intelligence controller.

[0007] By adopting the above technical solution, staff can control the digital microscope via a touch screen to observe the cell culture status in the culture dish in real time without removing the culture dish. The operation is simple, convenient, and safe. Alternatively, the digital microscope can be automatically controlled by an artificial intelligence controller to automatically observe the cell culture status in the culture dish. The artificial intelligence controller compares and analyzes the images captured by the digital microscope with a database to automatically control the temperature, humidity, carbon dioxide content, oxygen content, and nutrient addition within the main body of the equipment, reducing the workload of staff. During this process, the output of the first motor drives the first lead screw to rotate, causing the adjustment frame to drive the second motor, the second lead screw, the adjustment block, and the digital microscope to move back and forth. Then, the output of the second motor drives the second lead screw to rotate, causing the adjustment block to drive the digital microscope to move left and right. By adjusting the horizontal position of the digital microscope, it is possible to observe multiple culture dishes in real time and to conduct observations at various positions within the culture dish, offering high flexibility and a large observation area.

[0008] Furthermore, the adjusting frame is slidably connected to the support, and the adjusting frame is threadedly connected to the first lead screw.

[0009] By adopting the above technical solution, the first lead screw is driven to rotate by the output end of the first motor, which in turn causes the adjustment frame to drive the second motor, the second lead screw, the adjustment block and the digital microscope to move back and forth.

[0010] Furthermore, the adjusting block is slidably connected to the adjusting frame, and the adjusting block is threadedly connected to the second lead screw.

[0011] By adopting the above technical solution, the second lead screw is driven to rotate through the output end of the second motor, so that the adjusting block drives the digital microscope to move left and right.

[0012] Furthermore, the longitudinal section of the adjustment frame is H-shaped, and the longitudinal section of the adjustment block is T-shaped.

[0013] By adopting the above technical solution, the shape of the adjustment frame can prevent the adjustment frame from falling off the support, and the shape of the adjustment block can prevent the adjustment block and the digital microscope from falling off the adjustment frame.

[0014] Furthermore, the bottom of the digital microscope is parallel to the top of the partition.

[0015] By adopting the above technical solution, the space is divided by a partition, which facilitates the division of the internal space of the equipment. Furthermore, since the partition is parallel to the bottom of the digital microscope, it avoids collisions and scratches that could cause damage to the digital microscope.

[0016] Furthermore, a humidity control component and a culture component are respectively installed at the lower part of the main body of the equipment, a temperature control component is installed inside the main body of the equipment, gas control components are installed on both sides of the back of the main body of the equipment, and a sealing door and an inspection door are hinged on both sides of the outer surface of the main body of the equipment.

[0017] By adopting the above technical solution, the images captured by the digital microscope are compared and analyzed with the database through an artificial intelligence controller. At the same time, humidity sensors, temperature sensors, oxygen sensors and carbon dioxide sensors are used as sensing elements to measure various parameters of the internal environment of the main body of the equipment, thereby automatically controlling the temperature, humidity, carbon dioxide content, oxygen content and nutrient addition amount inside the main body of the equipment.

[0018] Furthermore, the humidity control assembly includes a dehumidifier and a humidifier located inside the lower part of the main body of the device. A drain pipe is connected to one side of the dehumidifier, and an exhaust pipe and an exhaust pipe are connected to the back of the dehumidifier. A water inlet pipe is connected to one side of the humidifier, and a spray pipe is connected to the back of the humidifier. A humidity sensor is installed inside the upper part of the main body of the device, and the dehumidifier, humidifier, and humidity sensor are all electrically connected to the artificial intelligence controller.

[0019] By adopting the above technical solution, when the humidity inside the equipment body is high, the dehumidifier starts to draw in air from the equipment body through the suction pipe, and then sends the dry air back to the equipment body through the return pipe, thereby reducing the humidity inside the equipment body. During this process, the moisture captured by the dehumidifier from the air is discharged through the drain pipe. Conversely, when the humidity inside the equipment body is low, soft water is supplied to the humidifier through the water inlet pipe. Then, the humidifier uses ultrasound to break the soft water into fine droplets and sends the droplets into the equipment body through the spray pipe, thereby increasing the humidity inside the equipment body.

[0020] Furthermore, the temperature control component includes a semiconductor cooler installed on the upper sides of both sides of the main body of the device. A heat spreader is fixed inside the main body of the device, and a partition is connected to the top of the heat spreader. A heater is installed at the bottom of the heat spreader. A temperature sensor is also installed on the upper side inside the main body of the device. The semiconductor cooler, the heater, and the temperature sensor are all electrically connected to the artificial intelligence controller.

[0021] By adopting the above technical solution, when the temperature inside the main body of the equipment is high, the semiconductor cooler is activated to cool the inside of the main body of the equipment; conversely, when the temperature inside the main body of the equipment is high, the heat spreader is heated by the heater, and then the heat is transferred to the inside of the main body of the equipment through the heat spreader, thereby raising the temperature inside the main body of the equipment.

[0022] Furthermore, the gas regulating component includes an exhaust pipe that runs through the middle of the top of the main body of the device, oxygen pipes and carbon dioxide pipes are respectively connected to the two sides of the back of the main body of the device, and oxygen sensors and carbon dioxide sensors are also installed inside the upper part of the main body of the device, and both oxygen sensors and carbon dioxide sensors are electrically connected to the artificial intelligence controller.

[0023] By adopting the above technical solution, when the oxygen content inside the main body of the equipment is low and the carbon dioxide content is high, oxygen is injected into the main body of the equipment through an oxygen pipe, and excess carbon dioxide inside the main body of the equipment is discharged through an exhaust pipe; conversely, when the carbon dioxide content inside the main body of the equipment is low and the oxygen content is high, carbon dioxide is injected into the main body of the equipment through a carbon dioxide pipe, and excess oxygen inside the main body of the equipment is discharged through an exhaust pipe.

[0024] Furthermore, the culture component includes a peristaltic pump installed inside the lower part of the main body of the device, and the liquid inlet end of the peristaltic pump is connected to an additive storage tank through a liquid extraction pipe. Both sides of the top of the heat spreader are connected to culture dishes, and the top of the culture dishes is connected to an end cap. An infusion pipe is connected between the top of the end cap and the liquid outlet end of the peristaltic pump, and the peristaltic pump is electrically connected to an artificial intelligence controller.

[0025] By adopting the above technical solution, when the nutrients such as serum and insulin in the culture dish are insufficient, the peristaltic pump extracts the nutrients from the additive storage tank through the extraction tube and delivers the nutrients into the culture dish through the infusion tube, thereby facilitating the provision of nutrients to the cells.

[0026] In summary, the present invention has the following main advantages:

[0027] 1. This utility model, through the setup of a digital microscope, an artificial intelligence controller, and a touch screen, allows operators to control the digital microscope via the touch screen, enabling real-time observation of cell culture conditions in culture dishes without removing the dishes. The operation is simple, convenient, and safe. Alternatively, the artificial intelligence controller can automatically control the digital microscope, automatically observing the cell culture conditions. The controller compares and analyzes the images captured by the digital microscope with a database, automatically controlling the temperature, humidity, carbon dioxide content, oxygen content, and nutrient addition within the equipment, reducing the workload of operators. Real-time observation is possible without removing the culture dishes, and various parameters of the intelligent control equipment can be automatically monitored.

[0028] 2. This utility model, through the setting of the observation components, drives the first lead screw to rotate through the output end of the first motor, causing the adjustment frame to drive the second motor, the second lead screw, the adjustment block, and the digital microscope to move back and forth. Then, the output end of the second motor drives the second lead screw to rotate, causing the adjustment block to drive the digital microscope to move left and right. By adjusting the horizontal position of the digital microscope, it is convenient to observe multiple petri dishes in real time and to conduct on-site observations at various positions within the petri dishes. It is highly flexible, has a large observation area, and is convenient for observation. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the structure of this utility model;

[0030] Figure 2 This is a schematic diagram of the back structure of this utility model;

[0031] Figure 3 This is a cross-sectional structural diagram of the present invention;

[0032] Figure 4 This is a schematic diagram of the support structure of this utility model;

[0033] Figure 5 This is a schematic diagram of the dehumidifier structure of this utility model;

[0034] Figure 6 This is a schematic diagram of the humidifier structure of this utility model;

[0035] Figure 7 This is a schematic diagram of the peristaltic pump structure of this utility model.

[0036] In the diagram: 1. Main body of the equipment; 2. Control components; 201. Artificial intelligence controller; 202. Touch screen; 3. Sealed door; 4. Inspection door; 5. Observation components; 501. Support frame; 502. First motor; 503. First lead screw; 504. Adjusting frame; 505. Second motor; 506. Second lead screw; 507. Adjusting block; 508. Digital microscope; 509. Partition; 6. Humidity control components; 601. Dehumidifier; 602. Drain pipe; 603. Exhaust pipe; 604. Return pipe; 605. Humidifier; 6 06. Water inlet pipe; 607. Spray humidifier pipe; 608. Humidity sensor; 7. Temperature control assembly; 701. Semiconductor cooler; 702. Heat spreader plate; 703. Heater; 704. Temperature sensor; 8. Gas control assembly; 801. Exhaust pipe; 802. Oxygen pipe; 803. Carbon dioxide pipe; 804. Oxygen sensor; 805. Carbon dioxide sensor; 9. Culture assembly; 901. Peristaltic pump; 902. Liquid extraction pipe; 903. Additive storage tank; 904. Infusion pipe; 905. Culture dish; 906. End cap. Detailed Implementation

[0037] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0038] The embodiments of this utility model will be described below based on its overall structure.

[0039] Example 1:

[0040] An intelligent real-time cell culture observation device, such as Figure 3 and Figure 4 As shown, it includes a main body 1, observation components 5 are arranged on both sides inside the main body 1, and a control component 2 is arranged on the lower side of one side of the main body 1;

[0041] Specifically, the observation component 5 includes a support 501 and a partition 509. A first motor 502 is mounted on one side of the outer surface of the support 501. A first lead screw 503 is connected to the output end of the first motor 502. An adjusting frame 504 is connected between the first lead screw 503 and the support 501. The adjusting frame 504 has an H-shaped longitudinal section and is slidably connected to the support 501. The adjusting frame 504 is threadedly connected to the first lead screw 503. A second motor 505 is mounted on one side of the adjusting frame 504. A second lead screw 506 is connected to the output end of the second motor 505. An adjusting block 507 is connected to the bottom of the second lead screw 506. The adjusting block 507 has a T-shaped longitudinal section and is slidably connected to the adjusting frame 504. The adjusting block 507 is threadedly connected to the second lead screw 506. The unit is equipped with a digital microscope 508. The bottom of the digital microscope 508 is coated with a PTFE film to prevent fogging caused by humidity inside the main body of the equipment. The bottom of the digital microscope 508 is parallel to the top of the partition 509. The output of the first motor 502 drives the first lead screw 503 to rotate, which causes the adjustment frame 504 to drive the second motor 505, the second lead screw 506, the adjustment block 507, and the digital microscope 508 to move back and forth. Then, the output of the second motor 505 drives the second lead screw 506 to rotate, which causes the adjustment block 507 to drive the digital microscope 508 to move left and right. By adjusting the horizontal position of the digital microscope 508, it is possible to conduct real-time observation of multiple petri dishes 905 and to conduct on-site observation of various positions within the petri dishes 905. It is highly flexible and has a large observation area.

[0042] Specifically, the control component 2 includes an artificial intelligence controller 201. The artificial intelligence controller 201 is tilted and equipped with a touch screen 202. The touch screen 202, the first motor 502, the second motor 505, and the digital microscope 508 are all electrically connected to the artificial intelligence controller 201. The operator can control the digital microscope 508 through the touch screen 202, thereby observing the cell culture status in the culture dish 905 in real time without removing the culture dish 905. The operation is simple, convenient, and safe. The artificial intelligence controller 201 can also automatically control the operation of the digital microscope 508, thereby automatically observing the cell culture status in the culture dish 905. The artificial intelligence controller 201 compares and analyzes the images captured by the digital microscope 508 with the database, thereby automatically controlling the temperature, humidity, carbon dioxide content, oxygen content, and nutrient addition amount inside the main body of the equipment 1, reducing the workload of the operator.

[0043] See Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 and Figure 7 In the above embodiment, a humidity control component 6 and a culture component 9 are respectively provided at the lower part of the inside of the main body 1, a temperature control component 7 is provided inside the main body 1, an air control component 8 is provided on both sides of the back of the main body 1, and a sealing door 3 and an inspection door 4 are hinged on both sides of the outer surface of the main body 1.

[0044] Specifically, the humidity control component 6 includes a dehumidifier 601 and a humidifier 605 located inside and below the main body 1. A drain pipe 602 is connected to one side of the dehumidifier 601, and an exhaust pipe 603 and an exhaust pipe 604 are connected to its back. A water inlet pipe 606 is connected to one side of the humidifier 605, and a spray pipe 607 is connected to its back. A humidity sensor 608 is installed inside and above the main body 1. The dehumidifier 601, humidifier 605, and humidity sensor 608 are all electrically connected to the artificial intelligence controller 201. When the humidity inside the main body 1 is high... When the dehumidifier 601 is activated, it draws in air from the main body 1 through the suction pipe 603 and then returns the dry air to the main body 1 through the return pipe 604, thereby reducing the humidity in the main body 1. During this process, the moisture captured by the dehumidifier 601 from the air is discharged through the drain pipe 602. Conversely, when the humidity in the main body 1 is low, soft water is supplied to the humidifier 605 through the water inlet pipe 606. The humidifier 605 then uses ultrasound to break the soft water into fine droplets and sends the droplets into the main body 1 through the spray pipe 607, thereby increasing the humidity in the main body 1.

[0045] Specifically, the temperature control component 7 includes a semiconductor cooler 701 installed on the upper sides of both sides of the main body 1, a heat spreader 702 fixed inside the main body 1, a partition 509 connected to the top of the heat spreader 702, a heater 703 installed at the bottom of the heat spreader 702, and a temperature sensor 704 installed on the upper inside of the main body 1. The semiconductor cooler 701, heater 703, and temperature sensor 704 are all electrically connected to the artificial intelligence controller 201. When the temperature inside the main body 1 is high, the semiconductor cooler 701 is activated to cool the inside of the main body 1; conversely, when the temperature inside the main body 1 is high, the heater 703 heats the heat spreader 702, and then the heat is transferred to the inside of the main body 1 through the heat spreader 702, thereby raising the temperature inside the main body 1.

[0046] Specifically, the gas regulating component 8 includes an exhaust pipe 801 that runs through the middle of the top of the main body 1. Oxygen pipes 802 and carbon dioxide pipes 803 are respectively connected to the two sides of the back of the main body 1. An oxygen sensor 804 and a carbon dioxide sensor 805 are also installed inside the upper part of the main body 1. Both the oxygen sensor 804 and the carbon dioxide sensor 805 are electrically connected to the artificial intelligence controller 201. When the oxygen content in the main body 1 is low and the carbon dioxide content is high, oxygen is injected into the main body 1 through the oxygen pipe 802, and excess carbon dioxide in the main body 1 is discharged through the exhaust pipe. Conversely, when the carbon dioxide content in the main body 1 is low and the oxygen content is high, carbon dioxide is injected into the main body 1 through the carbon dioxide pipe 803, and excess oxygen in the main body 1 is discharged through the exhaust pipe 801.

[0047] Specifically, the culture component 9 includes a peristaltic pump 901 installed inside the lower part of the main body 1. The inlet end of the peristaltic pump 901 is connected to an additive storage tank 903 through a suction pipe 902. Both sides of the top of the heat spreader 702 are connected to culture dishes 905. The top of the culture dish 905 is connected to an end cap 906. An infusion pipe 904 is connected between the top of the end cap 906 and the outlet end of the peristaltic pump 901. The peristaltic pump 901 is electrically connected to the artificial intelligence controller 201. When the nutrients such as serum and insulin in the culture dish 905 are insufficient, the peristaltic pump 901 extracts the nutrients from the additive storage tank 903 through the suction pipe 902 and delivers the nutrients into the culture dish 905 through the infusion pipe 904, thereby facilitating the provision of nutrients to the cells.

[0048] Example 2:

[0049] Based on the above embodiment one, in order to prevent the fluid from flowing freely, the following settings are now adopted.

[0050] See Figure 1 , Figure 2 , Figure 3 , Figure 5 and Figure 6In the above embodiments, solenoid valves are installed on the drain pipe 602, the exhaust pipe 603, the return pipe 604, the water inlet pipe 606, the humidification pipe 607, the exhaust pipe 801, the oxygen pipe 802, and the carbon dioxide pipe 803. The solenoid valves are electrically connected to the artificial intelligence controller 201, thereby facilitating the control of the opening and closing of the pipes and preventing the fluid from flowing randomly and affecting cell culture.

[0051] The implementation principle of this utility model is as follows: First, the staff opens the sealed door 3 and places the culture dish 905 containing cells and end cap 906 on the heat spreader 702. Then, the staff passes the infusion tube 904 through the top of the end cap 906. After that, the staff closes the sealed door 3.

[0052] Staff can control the digital microscope 508 via the touchscreen 202, allowing real-time observation of cell culture in the culture dish 905 without removing the dish. This operation is simple, convenient, and safe. Alternatively, the digital microscope 508 can be automatically controlled via the artificial intelligence controller 201, enabling automatic observation of cell culture in the dish 905. The controller 201 compares and analyzes the images captured by the digital microscope 508 with a database. Simultaneously, humidity sensor 608, temperature sensor 704, oxygen sensor 804, and carbon dioxide sensor 805 serve as sensing elements to measure various parameters of the internal environment of the main body of the device 1, thereby automatically... The device controls the temperature, humidity, carbon dioxide content, oxygen content, and nutrient addition within the main body 1 of the dynamic control equipment, reducing the workload of staff. During operation, the output of the first motor 502 drives the first lead screw 503 to rotate, causing the adjustment frame 504 to drive the second motor 505, the second lead screw 506, the adjustment block 507, and the digital microscope 508 to move back and forth. Then, the output of the second motor 505 drives the second lead screw 506 to rotate, causing the adjustment block 507 to drive the digital microscope 508 to move left and right. By adjusting the horizontal position of the digital microscope 508, it is possible to conduct real-time observation of multiple culture dishes 905 and to conduct on-site observation of various positions within the culture dishes 905. It is highly flexible and has a large observation area.

[0053] When the serum, insulin and other nutrients in the culture dish 905 are insufficient, the peristaltic pump 901 extracts the nutrients from the additive storage tank 903 through the extraction tube 902 and delivers the nutrients into the culture dish 905 through the infusion tube 904, thereby facilitating the provision of nutrients to the cells.

[0054] When the humidity inside the main body 1 is high, the dehumidifier 601 starts to draw in air from the main body 1 through the suction pipe 603, and then sends the dry air back to the main body 1 through the return pipe 604, thereby reducing the humidity inside the main body 1. During this process, the moisture captured by the dehumidifier 601 from the air is discharged through the drain pipe 602. Conversely, when the humidity inside the main body 1 is low, soft water is supplied to the humidifier 605 through the water inlet pipe 606. Then, the humidifier 605 uses ultrasound to break the soft water into fine droplets and sends the droplets into the main body 1 through the spray pipe 607, thereby increasing the humidity inside the main body 1.

[0055] When the temperature inside the main body 1 is high, the semiconductor cooler 701 is activated to cool the inside of the main body 1; conversely, when the temperature inside the main body 1 is high, the heat spreader 702 is heated by the heater 703, and then the heat is transferred to the inside of the main body 1 through the heat spreader 702, thereby raising the temperature inside the main body 1.

[0056] When the oxygen content in the main body 1 is low and the carbon dioxide content is high, oxygen is injected into the main body 1 through the oxygen pipe 802, and excess carbon dioxide in the main body 1 is discharged through the exhaust pipe; conversely, when the carbon dioxide content in the main body 1 is low and the oxygen content is high, carbon dioxide is injected into the main body 1 through the carbon dioxide pipe 803, and excess oxygen in the main body 1 is discharged through the exhaust pipe 801.

[0057] Solenoid valves are installed on the drain pipe 602, the exhaust pipe 603, the return pipe 604, the water inlet pipe 606, the humidification pipe 607, the exhaust pipe 801, the oxygen pipe 802, and the carbon dioxide pipe 803, so as to facilitate the control of the opening and closing of the pipes and avoid the situation where the fluid flows randomly and affects the cell culture.

[0058] It should be noted that the culture container is not limited to the culture dish 905; structures that can replace the culture dish 905 are also within the scope of protection.

[0059] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. An intelligent real-time cell culture observation device, comprising a main body (1), characterized in that: The main body (1) of the equipment has observation components (5) arranged on both sides inside; and the observation components (5) include a bracket (501) and a partition (509), and a first motor (502) is installed on one side of the outer surface of the bracket (501), the output end of the first motor (502) is connected to a first lead screw (503), and an adjustment frame (504) is connected between the first lead screw (503) and the bracket (501), a second motor (505) is installed on one side of the adjustment frame (504), and a second lead screw (506) is connected to the output end of the second motor (505), an adjustment block (507) is connected to the bottom of the second lead screw (506), and a digital microscope (508) is installed at the bottom of the adjustment block (507); a control component (2) is arranged below one side of the main body (1), and the control component (2) includes an artificial intelligence controller (201), and a touch screen (202) is installed at an angle on the artificial intelligence controller (201).

2. The intelligent real-time cell culture observation device according to claim 1, characterized in that: The adjusting frame (504) is slidably connected to the bracket (501), and the adjusting frame (504) is threadedly connected to the first lead screw (503).

3. The intelligent real-time cell culture observation device according to claim 2, characterized in that: The adjusting block (507) is slidably connected to the adjusting frame (504), and the adjusting block (507) is threadedly connected to the second lead screw (506).

4. The intelligent real-time cell culture observation device according to claim 3, characterized in that: The longitudinal section of the adjusting frame (504) is "H" shaped, and the longitudinal section of the adjusting block (507) is "T" shaped.

5. The intelligent real-time cell culture observation device according to claim 1, characterized in that: The bottom of the digital microscope (508) is parallel to the top of the partition (509).

6. The intelligent real-time cell culture observation device according to claim 1, characterized in that: The lower part of the main body of the equipment (1) is provided with a humidity control component (6) and a culture component (9). The main body of the equipment (1) is provided with a temperature control component (7). The main body of the equipment (1) is provided with air control components (8) on both sides of the back. The main body of the equipment (1) is provided with a sealing door (3) and an inspection door (4) on both sides of the outer surface.

7. The intelligent real-time cell culture observation device according to claim 6, characterized in that: The humidity control component (6) includes a dehumidifier (601) and a humidifier (605) located inside the lower part of the main body (1). A drain pipe (602) is connected to one side of the dehumidifier (601), and an exhaust pipe (603) and an exhaust pipe (604) are connected to the back of the dehumidifier (601). A water inlet pipe (606) is connected to one side of the humidifier (605), and a spray pipe (607) is connected to the back of the humidifier (605). A humidity sensor (608) is installed inside the upper part of the main body (1), and the dehumidifier (601), the humidifier (605), and the humidity sensor (608) are all electrically connected to the artificial intelligence controller (201).

8. The intelligent real-time cell culture observation device according to claim 7, characterized in that: The temperature control component (7) includes a semiconductor cooler (701) installed on both sides of the device body (1). A heat spreader (702) is fixed inside the device body (1), and a partition (509) is connected to the top of the heat spreader (702). A heater (703) is installed at the bottom of the heat spreader (702). A temperature sensor (704) is also installed inside the device body (1). The semiconductor cooler (701), heater (703) and temperature sensor (704) are all electrically connected to the artificial intelligence controller (201).

9. The intelligent real-time cell culture observation device according to claim 8, characterized in that: The gas regulating component (8) includes an exhaust pipe (801) that runs through the middle of the top of the main body (1). The back of the main body (1) is connected to an oxygen pipe (802) and a carbon dioxide pipe (803) respectively. An oxygen sensor (804) and a carbon dioxide sensor (805) are also installed inside the upper part of the main body (1). Both the oxygen sensor (804) and the carbon dioxide sensor (805) are electrically connected to the artificial intelligence controller (201).

10. The intelligent real-time cell culture observation device according to claim 9, characterized in that: The culture component (9) includes a peristaltic pump (901) installed inside the lower part of the main body (1), and the inlet end of the peristaltic pump (901) is connected to an additive storage tank (903) through a liquid extraction pipe (902). Both sides of the top of the heat spreader (702) are connected to culture dishes (905), and the top of the culture dish (905) is connected to an end cap (906). The top of the end cap (906) is connected to the outlet end of the peristaltic pump (901) through an infusion pipe (904), and the peristaltic pump (901) is electrically connected to an artificial intelligence controller (201).

Images