Algae bacteria-isolating circulating culture device

Abstract

The utility model relates to the technical field of algae culture, in particular to an algae bacteria-isolating circulating culture device which comprises a culture cavity, a multi-layer circulating module, a driving mechanism, a transmission shaft, a stirring assembly, a bacteria-isolating filtering unit and an intelligent regulation and control module. The multi-layer circulating module realizes uniform distribution of membrane waves through an annular flow guide plate, a circulating chamber and a flow dividing cone; the mixing effect of the stirring assembly is enhanced through a rotating disc, stirring blades and turbulent flow protrusions; the bacterium-isolating filtering unit adopts multiple layers of filtering membranes to realize strict bacterium isolation; and the intelligent regulation and control module realizes real-time monitoring and automatic regulation through a sensor group and a controller. The algae liquid mixing uniformity can be improved, the pollution risk is reduced, diversified culture requirements are met, meanwhile, the reliability of the device is improved, and the service life of the device is prolonged.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biological culture, specifically an algae bacteria-isolation circulating culture device. BACKGROUND

[0002] Algae culture devices have wide applications in the field of biotechnology, and their main function is to improve the growth efficiency of algae by optimizing the culture environment. However, the algae culture devices on the market still have certain limitations in complex culture environments. For example, traditional devices usually use simple circulation or aeration methods, and the bacteria-isolation effect is difficult to meet the efficient culture needs under sterile conditions, leading to low culture efficiency and increased pollution risk.

[0003] After searching, a kind of automatic algae bacteria-isolation culture device with publication number CN111718852B was disclosed on May 26, 2023. The device realizes automatic circulation of algae input and water in the culture system through control equipment, and maintains a sterile state throughout. However, it relies on a single injector for gas and liquid mixing, which can cause uneven mixing and affect the growth efficiency of algae. At the same time, the adaptability of this device to different types of algae is limited, making it difficult to meet the diversified culture needs.

[0004] After searching, a kind of algae culture system with publication number CN114292731B was disclosed on April 2, 2024. The design uses a wave-shaped culture tube structure, and the algae liquid circulates through a water pump, improving light utilization and promoting algae growth. However, its circulation system is relatively simple and lacks efficient bacteria-isolation measures, which may lead to the entry of pollutants into the culture system and increase the risk of contamination. In addition, the design lacks intelligent control capability for the culture environment, making it difficult to adjust parameters in real time to adapt to the growth needs of different algae.

[0005] The above problems show that the existing algae culture devices have certain limitations in meeting the needs of efficient circulation and strict bacteria-isolation in complex culture environments. Therefore, the utility model provides an intelligent and automated algae bacteria-isolation circulating culture device to overcome these shortcomings and provide a more efficient, intelligent, and adaptable solution to changing environments. SUMMARY

[0006] The utility model discloses an algae bacteria isolation circulating culture device, solve the technical problem that the algae culture device in the prior art is difficult to realize efficient circulation and strict bacteria isolation under complex culture environment. The traditional device usually relies on single injector to mix gas and liquid, which can easily lead to uneven mixing and affect the growth efficiency of algae. At the same time, the lack of efficient bacteria isolation measures in the circulation system can lead to the entry of pollutants into the culture system, increasing the risk of contamination. In addition, the existing design has poor adaptability to different types of algae and insufficient intelligent control capability, making it difficult to adjust parameters in real time to meet the diversified culture needs.

[0007] An algae bacteria isolation circulating culture device includes a culture cavity, a multilayer circulation module arranged in the culture cavity, a drive mechanism fixedly connected to the bottom of the culture cavity, a transmission shaft penetrating through the culture cavity and connected to the drive mechanism, a stirring assembly sleeved on the transmission shaft, a bacteria isolation filtration unit arranged outside the culture cavity, and an intelligent control module installed on the top of the culture cavity. The multilayer circulation module is fixed to the inner wall of the culture cavity by a support, the stirring assembly is located between the multilayer circulation modules, the bacteria isolation filtration unit is in communication with the culture cavity through a pipeline, and the intelligent control module is electrically connected to the drive mechanism and the bacteria isolation filtration unit through a signal line.

[0008] Further technical solutions are as follows: the multilayer circulation module includes a plurality of sets of annular flow guides, flow-through holes opened in the annular flow guides, and a flow splitting cone arranged inside the annular flow guides; the annular flow guides are arranged in a top-down stacking manner and are fixed to the inner wall of the culture cavity by bolts; the flow-through holes are uniformly distributed along the circumference of the annular flow guides; the flow splitting cone is fixed to the center of the annular flow guide and is connected to the annular flow guide by a support rod; the tip of the flow splitting cone faces upwards, and its surface is provided with a plurality of sets of inclined flow guide grooves. When the algae liquid flows from bottom to top, the flow splitting cone splits the algae liquid to the outside of the annular flow guide, and then forms a uniformly distributed circulating flow through the flow-through holes.

[0009] Further technical solutions are as follows: the stirring assembly includes a rotating disc sleeved on the transmission shaft, stirring blades uniformly distributed on the outer edge of the rotating disc, and turbulence protrusions arranged on the surface of the stirring blades; the number of stirring blades is more than three sets, and the inclination angle of each set of stirring blades is adjustable; the turbulence protrusions are semispherical and uniformly distributed along the length direction of the stirring blades. The rotating disc is fixedly connected to the transmission shaft by a key groove structure, and the stirring blades are installed on the rotating disc by a threaded interface, which facilitates the adjustment of the inclination angle of the stirring blades according to actual needs.

[0010] Further technical solutions are as follows: the bacteria isolation filtering unit comprises a shell, a plurality of layers of filtering membranes arranged inside the shell, and an air inlet and an air outlet installed at both ends of the shell; the plurality of layers of filtering membranes comprise, from inside to outside, a microporous filter membrane, an activated carbon adsorption layer, and an ultrafiltration membrane; the air inlet is communicated with an external air source through a first pipeline, and the air outlet is communicated with the culture cavity through a second pipeline; one-way valves are arranged on the first pipeline and the second pipeline; the shell is fixedly connected with the culture cavity through a flange structure, and the plurality of layers of filtering membranes are fixed to the inner wall of the shell through a buckle structure.

[0011] Further technical solutions are as follows: the intelligent control module comprises a shell, a controller arranged inside the shell, a display screen and operation buttons installed on the controller, and a sensor group electrically connected with the controller; the sensor group comprises a temperature sensor, an illumination sensor, a dissolved oxygen sensor, and a pH value sensor; the temperature sensor and the pH value sensor are inserted into the culture cavity through probes, the illumination sensor is fixed to the outer wall of the culture cavity through a support, and the dissolved oxygen sensor is in contact with the algal liquid in the culture cavity through a wire; the controller is electrically connected with the driving mechanism and the bacteria isolation filtering unit through signal lines, and is used for receiving data collected by the sensor group and outputting control instructions.

[0012] Further technical solutions are as follows: the driving mechanism comprises a motor, a speed reducer connected to the output shaft of the motor, and a shaft coupling fixedly connected to the output end of the speed reducer; the shaft coupling is fixedly connected with the transmission shaft through a pin; the motor is fixed to the bottom of the culture cavity through bolts, and the speed reducer is connected with the output shaft of the motor through a flange structure; and the transmission shaft is fixed to the bottom of the culture cavity through a bearing seat, so that the transmission shaft can be kept stable during high-speed rotation.

[0013] The technical solutions provided by the utility model have at least the following technical effects or advantages:

[0014] Due to the design of the multi-layer circulation module, the uniform distribution and efficient circulation of the algal liquid in the culture cavity are realized through the combination of the annular flow guide plate, the flow-through hole and the flow splitting cone. Specifically, the flow splitting cone splits the algal liquid to the outside of the annular flow guide plate, and then forms a uniformly distributed circulating flow through the flow-through hole, thereby improving the uniformity of the algal liquid mixing and the light utilization rate.

[0015] Due to the design of the stirring assembly, the sufficient mixing of the algal liquid is realized through the combination of the rotating disc, the stirring blade and the turbulence protrusion. The inclination angle of the stirring blade can be adjusted according to actual needs, and the turbulence protrusion further enhances the turbulent effect of the algal liquid, thereby avoiding the problem of low algal growth efficiency caused by uneven mixing in traditional devices.

[0016] Due to the adoption of the bacteria isolation filter unit design, strict bacteria isolation effect is realized through the combination of multiple layers of filter membranes. The synergistic effect of the microporous filter membrane, activated carbon adsorption layer and ultrafiltration membrane effectively prevents pollutants from entering the culture cavity, thereby reducing the risk of contamination and meeting the efficient culture demand under sterile conditions.

[0017] Due to the adoption of the intelligent control module design, real-time monitoring and automatic adjustment of the culture environment are realized through the combination of sensor groups and controllers. The synergistic work of temperature sensors, light sensors, dissolved oxygen sensors and pH sensors can accurately collect environmental parameters in the culture cavity, and the controller outputs control instructions according to the collected data, thereby meeting the diversified culture demand of different types of algae.

[0018] Due to the adoption of the drive mechanism and transmission shaft design, stable operation of the stirring assembly is realized through the combination of motors, reducers and couplings. The transmission shaft is fixed to the bottom of the culture cavity through the bearing seat, ensuring the stability of the transmission shaft during high-speed rotation, thereby improving the reliability and service life of the entire device. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0020] Figure 2 It is a schematic diagram of the structure of the multi-layer filter membrane.

[0021] Figure 3 It is a schematic diagram of the structure of the multi-layer circulation module.

[0022] Figure 4 It is a schematic diagram of the structure of the drive mechanism.

[0023] The reference signs are as follows:

[0024] 1, culture cavity; 2, multi-layer circulation module; 3, drive mechanism; 4, transmission shaft; 5, stirring assembly; 6, bacteria isolation filter unit; 7, intelligent control module; 8, annular flow guide plate; 9, flow-through hole; 10, flow dividing cone; 11, rotating disc; 12, stirring blade; 13, turbulence protrusion; 14, shell; 15, multi-layer filter membrane; 16, air inlet; 17, air outlet; 18, controller; 19, sensor group. DETAILED DESCRIPTION

[0025] The utility model provides an algae bacteria isolation circulation culture device, and the specific implementation mode combines the drawings of the utility model with the description of the utility model. Figure 1 to the drawings Figure 4Detailed description will be given. The device comprises a culture cavity 1, a multi-layer circulation module 2, a driving mechanism 3, a transmission shaft 4, a stirring assembly 5, a bacteria isolation and filtration unit 6 and an intelligent control module 7. The connection relationship, positional relationship and mutual cooperation relationship between each part will be described one by one in the following.

[0026] The culture cavity 1 is the core component of the whole device, which is used to contain the algae liquid and realize the cultivation of algae. The material of the culture cavity 1 is selected from transparent high-strength plastic or glass to ensure that the light can uniformly penetrate into the cavity. The multi-layer circulation module 2 is fixed on the inner wall of the culture cavity 1 by a support, and is arranged in a stacked manner and is tightly connected with the inner wall of the culture cavity 1 by bolts. The multi-layer circulation module 2 is composed of a ring-shaped flow guide plate 8, a flow-through hole 9 and a flow splitting cone 10. The ring-shaped flow guide plate 8 is in a ring structure, and its outer diameter matches the inner diameter of the culture cavity 1 to ensure the stability after installation. The flow-through holes 9 are uniformly distributed along the circumference of the ring-shaped flow guide plate 8, and their diameters are designed to be between 2 mm and 5 mm according to the flow characteristics of the algae liquid to ensure smooth flow of the algae liquid. The flow splitting cone 10 is located at the center of the ring-shaped flow guide plate 8 and is fixedly connected with the ring-shaped flow guide plate 8 by a support rod. The tip of the flow splitting cone 10 is upward, and the surface is provided with a plurality of inclined flow guide grooves. The design angle of these flow guide grooves is 30 to 45 degrees, which is used to guide the algae liquid to flow in a specific direction.

[0027] The stirring assembly 5 is sleeved on the transmission shaft 4 and located between the multi-layer circulation modules 2. The stirring assembly 5 comprises a rotating disc 11, stirring blades 12 and turbulence protrusions 13. The rotating disc 11 is fixedly connected with the transmission shaft 4 through a key groove structure to ensure that the transmission shaft 4 can drive the rotating disc 11 to rotate synchronously when the transmission shaft 4 rotates. The stirring blades 12 are uniformly distributed on the outer edge of the rotating disc 11, and the number of the stirring blades 12 is designed to be more than three. Each stirring blade 12 is installed on the rotating disc 11 through a threaded interface to facilitate adjusting the inclination angle of the stirring blade 12 according to actual needs. The inclination angle of the stirring blade 12 ranges from 15 degrees to 45 degrees, and the specific angle can be adjusted according to the viscosity and flowability of the algae liquid. The turbulence protrusions 13 are semispherical and are uniformly distributed along the length direction of the stirring blades 12. The diameter of the turbulence protrusions 13 is 3 mm to 8 mm, which is used to enhance the turbulent effect of the algae liquid to improve the mixing efficiency.

[0028] The driving mechanism 3 is fixedly connected to the bottom of the culture cavity 1. The driving mechanism 3 comprises a motor, a speed reducer and a shaft coupling. The motor is fixed to the bottom of the culture cavity 1 by bolts, and the output shaft of the motor is connected with the speed reducer through a flange structure. The output end of the speed reducer is fixedly connected with the transmission shaft 4 through the shaft coupling. The transmission shaft 4 penetrates through the culture cavity 1 and is fixed to the bottom of the culture cavity 1 through a bearing seat. The design of the bearing seat ensures that the transmission shaft 4 remains stable when rotating at high speed. The top of the transmission shaft 4 extends into the culture cavity 1 and is connected with the stirring assembly 5. When the motor is started, the transmission shaft 4 drives the stirring assembly 5 to rotate, thereby realizing the sufficient mixing of the algae liquid.

[0029] The bacteria isolation and filtration unit 6 is arranged outside the culture cavity 1 and communicates with the culture cavity 1 through a pipeline. The bacteria isolation and filtration unit 6 comprises a shell 14, a multi-layer filtration membrane 15, an air inlet 16 and an air outlet 17. The shell 14 is fixedly connected with the culture cavity 1 through a flange structure, and the inside of the shell 14 is provided with the multi-layer filtration membrane 15. The multi-layer filtration membrane 15 comprises, from inside to outside, a microporous filter membrane, an activated carbon adsorption layer and an ultrafiltration membrane. The microporous filter membrane has a pore size of 0.2 microns and is used for preliminarily filtering particulate matters in the air; the activated carbon adsorption layer is used for adsorbing harmful gases and odors; and the ultrafiltration membrane has a pore size of 0.01 microns and is used for further filtering microorganisms and bacteria. The air inlet 16 communicates with an external air source through a first pipeline, and the air outlet 17 communicates with the culture cavity 1 through a second pipeline. Unidirectional valves are arranged on the first pipeline and the second pipeline to prevent the algae liquid in the culture cavity 1 from flowing back to the bacteria isolation and filtration unit 6.

[0030] The intelligent control module 7 is installed on the top of the culture cavity 1, and the shell of the intelligent control module 7 is provided with a controller 18, a display screen, operation buttons and a sensor group 19. The sensor group 19 comprises a temperature sensor, an illumination sensor, a dissolved oxygen sensor and a pH value sensor. The temperature sensor and the pH value sensor are inserted into the culture cavity 1 through probes and are used for monitoring the temperature and the pH value of the algae liquid in real time. The illumination sensor is fixed to the outer wall of the culture cavity 1 through a support and is used for detecting the illumination intensity. The dissolved oxygen sensor is in contact with the algae liquid in the culture cavity 1 through a wire and is used for monitoring the dissolved oxygen content in the algae liquid. The controller 18 is electrically connected with the driving mechanism 3 and the bacteria isolation and filtration unit 6 through signal lines, receives the data collected by the sensor group 19 and outputs control instructions. For example, when the temperature sensor detects that the temperature of the algae liquid is lower than a set value, the controller 18 will start the heating device to increase the temperature of the algae liquid; and when the dissolved oxygen sensor detects that the dissolved oxygen content in the algae liquid is insufficient, the controller 18 will supplement oxygen into the culture cavity 1 through the bacteria isolation and filtration unit 6.

[0031] In actual operation, the algae liquid enters from the bottom of the culture cavity 1 and rises layer by layer through the multi-layer circulation module 2. The shunt cone 10 divides the algae liquid into the outside of the annular flow guide plate 8, and then forms a uniform circulating flow through the flow-through holes 9. At the same time, the driving mechanism 3 drives the transmission shaft 4 to rotate, and the stirring assembly 5 on the transmission shaft 4 rotates accordingly. The stirring blades 12 and the turbulence protrusions 13 jointly produce a turbulent effect on the algae liquid, so as to realize sufficient mixing of the algae liquid. The bacteria isolation and filtration unit 6 strictly filters the air entering the culture cavity 1 through the multi-layer filtration membrane 15, so as to ensure efficient culture under sterile conditions. The intelligent control module 7 adjusts the culture environment parameters, such as the temperature, the illumination intensity, the dissolved oxygen content and the pH value, in real time according to the data collected by the sensor group 19, so as to meet the diversified culture requirements of different types of algae.

[0032] It can be seen from the above specific embodiments that the algal bacteria-isolation circulating culture device provided by the utility model realizes efficient circulation, strict bacteria isolation and intelligent control of algal liquid through the synergistic effect of the multi-layer circulating module 2, the stirring assembly 5, the bacteria-isolation filtration unit 6 and the intelligent control module 7, and solves the technical problem that the algal culture device in the prior art is difficult to realize efficient circulation and strict bacteria isolation under a complex culture environment.

[0033] In order to make the relevant personnel in the technical field better understand and realize the utility model, the specific implementation principle of the utility model is further supplemented in the following by combining with one specific application scene.

[0034] In the initial stage, an appropriate amount of algal liquid is injected into the culture cavity 1, and the height of the algal liquid needs to ensure that it covers the bottommost annular flow guide plate 8 of the multi-layer circulating module 2. The initial parameters, including the target ranges of temperature, light intensity, dissolved oxygen content and pH value, are set through the operation keys of the intelligent control module 7. The controller 18 starts the motor in the driving mechanism 3 according to the preset parameters, the motor output shaft drives the transmission shaft 4 to rotate through the speed reducer and the shaft coupling, so that the stirring assembly 5 starts to work. The rotating disc 11 rotates synchronously with the transmission shaft 4, the stirring blade 12 forms a preliminary stirring in the algal liquid, and the turbulence protrusion 13 further enhances the turbulence effect, so as to promote the rapid mixing of the algal liquid in the culture cavity 1.

[0035] Subsequently, the algal liquid is pushed to flow upwards under the action of the stirring assembly 5 and enters the first layer of annular flow guide plates 8 of the multi-layer circulating module 2. The flow splitter 10 guides the algal liquid along the surface of the flow guide groove to the outside of the annular flow guide plate 8, and then the algal liquid is uniformly distributed to the next layer of annular flow guide plates 8 through the flow-through holes 9. In this process, the design of the annular flow guide plate 8 ensures that the algal liquid can flow along a fixed path, avoiding local retention or short-circuit phenomenon. In the process of ascending layer by layer, the algal liquid forms a stable circulating flow between each layer of annular flow guide plates 8, which significantly improves the mixing uniformity and light utilization rate of the algal liquid.

[0036] At the same time, the bacteria-isolation filtration unit 6 starts to work, the external air enters the inside of the shell 14 through the air inlet 16, and is subjected to multi-stage filtration treatment through the microporous filter membrane, the activated carbon adsorption layer and the ultrafiltration membrane in sequence. The microporous filter membrane effectively intercepts particulate matter with a diameter greater than 0.2 microns in the air, the activated carbon adsorption layer adsorbs harmful gases and odors, and the ultrafiltration membrane further filters microorganisms and bacteria, so as to ensure that the air entering the culture cavity 1 reaches the sterile standard. The one-way valves on the first pipeline and the second pipeline prevent the algal liquid from flowing back to the bacteria-isolation filtration unit 6, thereby ensuring the safety of the whole system.

[0037] During the cultivation process, the sensor group 19 monitors the environmental parameters in the cultivation cavity 1 in real time. The temperature sensor detects the temperature of the algal liquid and transmits data to the controller 18 through a signal line. If the temperature is lower than the set value, the controller 18 starts the heating device to raise the temperature of the algal liquid. The light sensor detects the light intensity of the outer wall of the cultivation cavity 1. When the light is insufficient, the controller 18 adjusts the power of the external light source to meet the growth needs of the algae. The dissolved oxygen sensor detects the dissolved oxygen content in the algal liquid. If the content is insufficient, the controller 18 supplements oxygen to the cultivation cavity 1 through the sterile filtration unit 6. The pH sensor detects the acidity and alkalinity of the algal liquid. When the pH value deviates from the target range, the controller 18 controls the external acid-base adjusting device to make corresponding adjustment.

[0038] As the cultivation time is prolonged, the algal liquid continuously maintains a high-efficiency circulation state under the synergistic action of the multi-layer circulation module 2 and the stirring assembly 5. The combination design of the diverging cone 10 and the annular flow guide plate 8 ensures that the algal liquid forms a stable laminar flow structure in the cultivation cavity 1, and the stirring blade 12 and the turbulence protrusion 13 further optimize the mixing effect of the algal liquid. The sterile filtration unit 6 continuously provides sterile air for the cultivation cavity 1, avoiding the invasion of pollutants. The intelligent control module 7 dynamically adjusts the cultivation environmental parameters according to the data collected by the sensor group 19, ensuring that the algae are always in the best growth conditions.

[0039] Finally, when the algal growth cycle is over, the motor in the driving mechanism 3 is turned off, and the operation of the stirring assembly 5 is stopped. The algal liquid is discharged through the discharge port at the bottom of the cultivation cavity 1, completing a complete cultivation process. During the entire cultivation period, the algal sterile circulation cultivation device provided by the present application realizes efficient circulation, strict sterilization and intelligent control of the algal liquid through the synergistic action of the multi-layer circulation module 2, the stirring assembly 5, the sterile filtration unit 6 and the intelligent control module 7, solving the technical problems that the algal cultivation device in the prior art is difficult to realize efficient circulation and strict sterilization in a complex cultivation environment.

[0040] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. An algae culture device with cyclic bacterial circulation, characterized in that, The system includes a culture chamber (1), a multi-layer circulation module (2) disposed within the culture chamber (1), a drive mechanism (3) fixedly connected to the bottom of the culture chamber (1), a transmission shaft (4) passing through the culture chamber (1) and connected to the drive mechanism (3), a stirring assembly (5) sleeved on the transmission shaft (4), a microbial filtration unit (6) disposed outside the culture chamber (1), and an intelligent control module (7) installed on the top of the culture chamber (1). The multi-layer circulation module (2) is fixed to the inner wall of the culture chamber (1) by a bracket, the stirring assembly (5) is located between the multi-layer circulation modules (2), the microbial filtration unit (6) is connected to the culture chamber (1) through a pipe, and the intelligent control module (7) is electrically connected to the drive mechanism (3) and the microbial filtration unit (6) through a signal line.

2. The algae-isolated bacterial circulation culture device as described in claim 1, characterized in that, The multi-layer circulation module (2) includes several sets of annular guide plates (8), flow holes (9) opened on the annular guide plates (8), and flow divider cones (10) disposed on the inner side of the annular guide plates (8). The annular guide plates (8) are stacked vertically and fixed to the inner wall of the culture chamber (1) by bolts. The flow holes (9) are evenly distributed along the circumference of the annular guide plates (8). The flow divider cones (10) are fixed at the center position of the annular guide plates (8) and connected to the annular guide plates (8) by support rods. The tip of the flow divider cones (10) faces upward and its surface is provided with several sets of oblique flow guide grooves.

3. The algae culture device with bacterial circulation as described in claim 1, characterized in that, The stirring assembly (5) includes a rotating disk (11) sleeved on the drive shaft (4), stirring blades (12) evenly distributed on the outer edge of the rotating disk (11), and turbulence protrusions (13) provided on the surface of the stirring blades (12); the number of stirring blades (12) is three or more, and the tilt angle of each set of stirring blades (12) is adjustable; the turbulence protrusions (13) are hemispherical and evenly distributed along the length direction of the stirring blades (12); the rotating disk (11) is fixedly connected to the drive shaft (4) through a keyway structure; and the stirring blades (12) are installed on the rotating disk (11) through a threaded interface.

4. The algae culture device with bacterial circulation as described in claim 1, characterized in that, The bacterial filtration unit (6) includes a housing (14), a multi-layer filter membrane (15) disposed inside the housing (14), and an air inlet (16) and an air outlet (17) installed at both ends of the housing (14). The multi-layer filter membrane (15) consists of a microporous filter membrane, an activated carbon adsorption layer, and an ultrafiltration membrane from the inside to the outside. The air inlet (16) is connected to an external air source through a first pipe, and the air outlet (17) is connected to the culture chamber (1) through a second pipe. Both the first pipe and the second pipe are equipped with one-way valves.

5. The algae culture device with bacterial circulation as described in claim 1, characterized in that, The intelligent control module (7) includes a housing, a controller (18) disposed inside the housing, a display screen and operation buttons mounted on the controller (18), and a sensor group (19) electrically connected to the controller (18). The sensor group (19) includes a temperature sensor, a light sensor, a dissolved oxygen sensor, and a pH sensor. The temperature sensor and the pH sensor are inserted into the culture chamber (1) through probes. The light sensor is fixed to the outer wall of the culture chamber (1) through a bracket. The dissolved oxygen sensor is in contact with the algal solution in the culture chamber (1) through a wire.

6. The algae culture device with bacterial circulation as described in claim 1, characterized in that, The drive mechanism (3) includes a motor, a reducer connected to the output shaft of the motor, and a coupling fixedly connected to the output end of the reducer; the coupling is fixedly connected to the transmission shaft (4) by a pin, the motor is fixed to the bottom of the culture chamber (1) by bolts, the reducer is connected to the output shaft of the motor by a flange structure, and the transmission shaft (4) is fixed to the bottom of the culture chamber (1) by a bearing seat.

7. The algae-isolated bacterial circulation culture device as described in claim 2, characterized in that, The diameter of the flow hole (9) is 2 mm to 5 mm, and the angle of the inclined guide groove on the surface of the flow divider cone (10) is 30 degrees to 45 degrees.

8. The algae-isolated bacterial circulation culture device as described in claim 3, characterized in that, The tilt angle of the stirring blade (12) ranges from 15 degrees to 45 degrees, and the diameter of the turbulence protrusion (13) ranges from 3 mm to 8 mm.

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