In-vitro cell co-culture device
By adjusting the spacing of the telescopic rods, the problem of the inability to flexibly control the cell ratio in existing co-culture devices has been solved, achieving flexible cell ratio adjustment and stable experimental results, thus meeting the needs of in-depth research.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-14
- Publication Date
- 2026-03-20
AI Technical Summary
Existing co-culture devices cannot flexibly control different cell ratios, and the upper chamber area is fixed, which cannot meet the needs of in-depth research.
By adjusting the spacing between the telescopic rods, co-culture chambers of different sizes can be accommodated and placed, enabling flexible control of cell ratios.
It improves the flexibility of the device, can meet the experimental needs of various cell ratios, reduces batch variability in experimental results, and saves time and costs.
Smart Images
Figure CN224015688U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture technical field, especially in vitro cell co -culture device. BACKGROUND
[0002] Cell co -culture can simulate the in -vivo growth environment more truly, and further reflects the interaction between cells, and is widely used in biomedical field to promote tumor microenvironment and drug development research. The existing co -culture device mainly is through the culture of two cells in the small room and the bottom culture dish respectively, and the small room is placed in the upper chamber formed in the culture dish upper, makes two cells in the same space co -culture, reaches the purpose of observing the interaction, signal transmission and metabolic process between two cells.
[0003] At present, the co -culture device of two cells has been commercialized, but as researchers continue to understand the mechanism of the interaction between various systems of human body, the co -culture device of two cells cannot meet the research needs of this kind, and the area of the upper chamber of the double cell co -culture device is fixed, cannot control the co -culture of different cell quantity ratio, and has the problem of low use flexibility. SUMMARY
[0004] In view of the above technical problems, the utility model provides an in vitro cell co -culture device, through the spacing between telescopic rod is adjusted, can supply the different size co -culture small room to place, has the advantage that the use flexibility is higher.
[0005] To achieve the above object, the utility model takes the following technical scheme:
[0006] An in vitro cell co -culture device, comprising:
[0007] Culture dish, the top of culture dish is open, forms the dish mouth, a plurality of limit grooves are arranged on the circumference of the dish mouth at intervals;
[0008] The dish cover is covered on the dish mouth; and
[0009] Telescopic rod, both ends of telescopic rod are hung on the limit groove, and the telescopic rod is provided with at least two, and the telescopic rod forms the receiving cavity for the culture dish to be arranged between two telescopic rods.
[0010] In one of the embodiments, both ends of the telescopic rod are provided with barbs, and the barbs limit the telescopic rod to be horizontally separated from the limit groove.
[0011] In one of the embodiments, the outer edge of the culture dish is fixedly provided with a convex edge, the dish cover is sleeved on the outside of the culture dish, and the bottom of the dish cover is horizontally attached to the convex edge.
[0012] In one embodiment, the telescopic rod comprises a fixed section and a movable section, the fixed section is sleeved outside the movable section, and the fixed section is threadedly connected with the movable section.
[0013] In one embodiment, the inner wall of the culture dish is provided with a ring, and the ring is used for accommodating the culture dish.
[0014] In one embodiment, the ring is 10 mm away from the bottom of the culture dish.
[0015] In one embodiment, the ring is provided with four rings, and the rings are uniformly and circumferentially spaced.
[0016] The utility model discloses the following advantages are obtained by adopting the above technical scheme.
[0017] When using the device, the cover is removed, and the required culture dishes are placed inside according to the requirements. Then, the operator can adjust the distance between the two telescopic rods according to the specific structure and size of the co-culture chamber. After adjustment, the two ends of the telescopic rod may not match the dish opening. At this time, the overall length of the telescopic rod needs to be adjusted to match. After adjustment, the co-culture chamber can be placed in the accommodation cavity formed between the two telescopic rods, and the outer edge of the co-culture chamber can be erected on the two telescopic rods. After all the culture dishes are accommodated and placed, the cover can be closed again. Compared with the prior art, the device can accommodate and place co-culture chambers of different sizes by adjusting the distance between the telescopic rods, and has the advantage of high flexibility. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 It is an external structure diagram of the culture dish in an embodiment of the utility model;
[0019] Figure 2 It is a structure diagram of the culture dish in an embodiment of the utility model;
[0020] Figure 3 It is a top view of the culture dish in an embodiment of the utility model;
[0021] Figure 4 It is a specific structure diagram of the telescopic rod in an embodiment of the utility model;
[0022] Figure 5 It is a use state diagram of the culture dish in an embodiment of the utility model;
[0023] REFERENCE SIGNS:
[0024] 1, culture dish; 2, dish cover; 3, telescopic rod; 4, dish mouth; 5, barb; 6, limiting groove; 7, convex edge; 8, ring. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical scheme and advantages of the utility model more clear, the technical scheme in the utility model is described clearly and completely below, obviously, the described embodiment is a part of the embodiment of the utility model, rather than all the embodiment. Based on the embodiment in the utility model, all other embodiments obtained by the person skilled in the art without making creative labor belong to the scope of protection of the utility model.
[0026] Unless otherwise defined, the technical terms or scientific terms used in the utility model should be the usual meaning understood by the person skilled in the art in the field to which the utility model belongs. The "first", "second", "third", "fourth" and similar words used in the utility model do not represent any order, quantity or importance, but are only used to distinguish different components. "Include" or "contain" and similar words mean that the elements or objects before the word cover the elements or objects listed after the word and its equivalent, and do not exclude other elements or objects. "Connection" or "connection" and similar words are not limited to physical or mechanical connection, but can include electrical connection, whether direct or indirect.
[0027] Cell co-culture can more truly simulate in-vivo growth environment, further reflect the interaction between cells, and is widely used in the biomedical field to promote tumor microenvironment and drug development research. At present, the co-culture device of two kinds of cells has been commercialized, but as researchers continuously deepen the understanding of the mechanism of action between various systems of human body, the co-culture device of two kinds of cells cannot meet the research needs of the kind; and the area of the upper chamber of the double-cell co-culture device is fixed, the co-culture of different cell proportions cannot be controlled, and there is a problem of low use flexibility. In view of the above technical problems, the utility model provides an in-vitro cell co-culture device, which can accommodate and place co-culture chambers of different sizes by adjusting the distance between the telescopic rods, has the advantage of high use flexibility, and the technical scheme of the utility model will be described in detail below in combination with specific examples.
[0028] Referring to Figure 1 , Figure 2 and Figure 3 , the utility model relates to an in-vitro cell co-culture device, which comprises a culture dish 1, a dish cover 2 and a telescopic rod 3, wherein the top of the culture dish 1 is open to form a dish mouth 4, a plurality of limiting grooves 6 are arranged circumferentially at intervals in the dish mouth 4, and the dish cover 2 covers the dish mouth 4.
[0029] The two ends of the telescopic rod 3 are hung on the limiting groove 6, and the telescopic rod 3 is provided with at least two telescopic rods 3, and the two telescopic rods 3 form a receiving cavity for arranging the culture vessel.
[0030] In an embodiment, the overall structure of the telescopic rod 3 is further refined, and the telescopic rod 3 includes a fixed section and a movable section, the fixed section is sleeved outside the movable section, and the fixed section is threadedly connected with the movable section.
[0031] Exemplarily, when using the device, the dish cover 2 needs to be removed, and the required culture vessel is placed in the inside of the culture dish 1 according to the need, then the operator can adjust the distance between the two telescopic rods 3 according to the specific structure size of the co-culture chamber, after the adjustment is completed, the two ends of the telescopic rod 3 may not match the dish mouth 4, at this time, the overall length of the telescopic rod 3 needs to be adjusted to adapt, after the adjustment is completed, the co-culture chamber can be placed in the receiving cavity formed between the two telescopic rods 3, and the outer edge of the co-culture chamber can be arranged on the two telescopic rods 3, after the receiving and placing of all the culture vessels are completed, the dish cover 2 can be covered again, compared with the prior art, the device can accommodate different sizes of co-culture chambers by adjusting the distance between the telescopic rods 3, and has the advantage of high flexibility.
[0032] It should be noted that, because the limiting groove 6 is circumferentially provided with a plurality of limiting grooves 6, in order to further adapt to a plurality of co-culture chambers, the telescopic rod 3 can be configured with a plurality of telescopic rods 3 according to actual needs, and the specific use is mainly based on the actual use.
[0033] Among them, the plurality of telescopic rods 3 form a storage rack with a receiving cavity, and the existence of the storage rack ensures that the same experimental system can simultaneously place a plurality of different or same polycarbonate pore size chambers, when the chamber pore size is small, the cells are not allowed to pass through, and the mutual influence of secreted substances between cells can be detected. When the polycarbonate membrane pore size is large, the migration and invasion ability of the upper chamber cells under the influence of the lower chamber cells can be detected. Different detection indexes of the upper chamber cells can be completed under the same batch and condition, and the difference between different batches and groups is reduced.
[0034] In addition, the co-culture chamber is placed in the square cavity of the storage rack, and when a plurality of chambers are placed, the adjacent chambers are individually hung, and there is no contact between the chambers, so there is no need to worry about the cross growth of cells in different adjacent chambers.
[0035] Referring to Figure 4 In an embodiment, more preferably, the two ends of the telescopic rod 3 are provided with barbs 5, and the barbs 5 limit the telescopic rod 3 from being horizontally separated from the limiting groove 6.
[0036] It should be noted that after the position adjustment of the telescopic rod is completed, the overall length of the telescopic rod 3 needs to be adjusted for adaptation, at which time the barb 5 can be hung on the limiting groove 6 to limit the telescopic rod 3 from being horizontally separated from the limiting groove 6, thereby playing a role in positioning and fixing the telescopic rod 3 and providing overall use stability of the device.
[0037] In an embodiment, the overall structure of the culture dish 1 is further refined, and a raised edge 7 is fixedly arranged at the outer edge of the culture dish 1. The dish cover 2 is sleeved outside the culture dish 1, and the bottom of the dish cover 2 is horizontally attached to the raised edge 7.
[0038] The raised edge 7 not only plays a role in positioning the dish cover 2, but also further optimizes the sealing performance of the dish cover 2 by forming a horizontal attachment between the top surface of the raised edge 7 and the bottom of the dish cover 2. At the same time, by arranging the raised edge 7, the operator can easily pick up and place the culture dish 1, increasing the convenience and safety of picking up and placing the co-culture device.
[0039] Referring to Figure 2 , Figure 3 and Figure 4 , in an embodiment, more preferably, a circular ring 8 is arranged on the inner wall of the culture dish 1, and the circular ring 8 is used for accommodating and placing the culture vessel.
[0040] During the experiment, the operator can place the culture vessel in the middle of the culture dish 1 according to the actual needs. Specifically, in this embodiment, the circular ring 8 is 10 mm away from the bottom of the culture dish 1. In addition, in order to further optimize the use flexibility of the device, four circular rings 8 are arranged uniformly along the circumference of the culture dish 1.
[0041] It should be noted that there is a sufficient height difference between the co-culture chamber, the side wall hanging small dish, and the bottom large culture dish 1 of the device, and the shelf is a hollow device. Therefore, when co-culturing multiple cells, the cells in the upper, middle, and lower chambers can be laid down simultaneously without moving the chamber, and the cells can be collected in the order of upper, middle, and lower when the co-culture is completed, preventing contamination caused by moving the chamber.
[0042] In some extended embodiments, the circular ring 8 is an adjustable inner diameter binding cord. Specifically, the locking head part of the binding cord is fixed to the inner wall of the culture dish 1, and the tail part of the binding cord is inserted into the locking head to form a ring as a whole, so as to accommodate and place the culture vessel. At the same time, by pulling the rack part of the binding cord, the inner diameter of the circular ring 8 can be adjusted to flexibly adapt to the culture vessel.
[0043] Exemplarily, the small dish fixed on the ring 8 can be used for co-culturing multiple cells, and the bottom of the hanging small dish can be selected from a non-porous plastic material, the same kind of cells are planted in the bottom dish and the side wall small dish, and subsequent observation of different indexes of one kind of cell is realized, for example, the protein expression localization is observed by immunofluorescence observation of cell climbing sheet, the expression content of the target protein is observed by Western blot of extracted cell protein, the expression level of the target RNA is observed by QPCR technology of extracted RNA, the problem that the bottom cells in the existing co-culturing system can only observe a single index is solved, the defect that different indexes need to be detected by multiple batches of experiments is solved, the batch effect of experimental results can be avoided by the structure, and time and money cost are further saved. In addition, since the height difference between the side wall small dish and the bottom culture dish 1 is sufficient, cell transfection in an independent space can be realized first, and co-culturing experiment is carried out after transfection is completed.
[0044] Specifically, the specific mounting structure is that a vertical hole is formed on the inner wall of the culture dish 1, so that the binding rope can be inserted into the binding installation.
[0045] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.
Claims
1. An in vitro cell co-culture device, characterized in that, include: A petri dish, the top of which is open to form a mouth, and the mouth of which is provided with multiple limiting grooves at intervals around its circumference; A lid that fits over the mouth of the dish; as well as The telescopic rod has its two ends hung on the limiting groove. At least two telescopic rods are provided, and a storage cavity for the culture vessel is formed between the two telescopic rods.
2. The in vitro cell co-culture device according to claim 1, characterized in that, Both ends of the telescopic rod are provided with barbs, which prevent the telescopic rod from horizontally disengaging from the limiting groove.
3. The in vitro cell co-culture device according to claim 1, characterized in that, The outer edge of the culture dish is fixedly provided with a raised edge, and the dish lid is fitted over the outside of the culture dish, with the bottom of the dish lid horizontally fitting against the raised edge.
4. The in vitro cell co-culture device according to claim 1, characterized in that, The telescopic rod includes a fixed section and a movable section. The fixed section is sleeved on the outside of the movable section, and the fixed section is threadedly connected to the movable section.
5. The in vitro cell co-culture device according to claim 1, characterized in that, A ring is provided on the inner wall of the culture dish for storing and placing the culture dish.
6. The in vitro cell co-culture device according to claim 5, characterized in that, The ring is 10 mm away from the bottom of the culture dish.
7. The in vitro cell co-culture device according to claim 5, characterized in that, Four rings are provided and are evenly spaced along the circumference of the culture dish.