Cell glass slide device

Abstract

The utility model discloses a cell slide device. The cell slide device comprises a culture dish 10, a sealing ring, a cell slide and a base which are sequentially arranged from top to bottom, wherein the culture dish 10 is of a hollow through structure, the culture dish comprises a culture dish upper section and a culture dish lower section, the culture dish upper section and the culture dish lower section are coaxially arranged, the cross section area of the culture dish upper section is a, the cross section area of the culture dish lower section is b, and a is smaller than b; the culture dish is detachably connected with the base through the culture dish lower section. The cell climbing slide device has the beneficial effects that the cell climbing slide device can overcome the two defects, so that cells can be directly cultured on the climbing slide, and the climbing slide can be quickly taken out for subsequent experiments after the culture is completed.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical instrument technical field especially relates to a cell climbing slide device. BACKGROUND

[0002] Cell climbing slide is to immerse slide in cell culture medium, so that cells grow on slide, and subsequent experiment can directly take out slide for use, which avoids damage of cells from culture plate to slide.

[0003] The existing cell climbing slide is used by first dropping a small amount of culture medium in each hole to prepare a position for climbing slide, so that slide and culture dish are adhered together by the tension of culture medium, and then slide is placed.

[0004] CN 204265761U discloses a cell climbing slide culture device, which comprises a slide plate (1), a plurality of grooves (2) arranged in order on the slide plate (1), a slide (3) arranged in the groove (2) and matched with the shape of the bottom of the groove (2), a slide taking notch (4) arranged on the side wall of the groove (2), and a handle (5) arranged in the slide taking notch (4) and matched with the slide (3).

[0005] The device of the prior art and the operation process realized by the matching device are relatively complex and are easy to be contaminated.

[0006] In summary, the utility model discloses a cell climbing slide device which can overcome the above two shortcomings, so that cells can be directly cultured on climbing slide, and climbing slide can be quickly taken out for subsequent experiment after culture. UTILITY MODEL CONTENTS

[0007] The utility model aims at solving the shortcomings in the prior art and provides a cell sorting and separating tube set.

[0008] In order to achieve the above object, the utility model adopts the following technical scheme:

[0009] A cell climbing slide device comprises, from top to bottom, a culture dish 10, a sealing ring, a cell climbing slide and a base.

[0010] The culture dish 10 is a hollow through structure, and comprises a culture dish upper segment and a culture dish lower segment which are coaxially arranged, and the cross-sectional area of the culture dish upper segment is a, and the cross-sectional area of the culture dish lower segment is b, and the quantity relationship a

[0011] The culture dish is detachably connected with the base through the lower section of the culture dish.

[0012] As a further preferred scheme, the upper section of the culture dish is an upper section cylinder, and the lower section of the culture dish is a lower section cylinder.

[0013] The culture dish is an axially-through hollow stepped cylinder composed of the upper section cylinder and the lower section cylinder coaxially arranged.

[0014] The diameter of the upper section cylinder is smaller than that of the lower section cylinder, and the two section cylinders are connected through a radial mutation surface to form a stepped transition, and the overall axial direction keeps the through channel.

[0015] As a further preferred scheme, the culture dish is an integrally formed structure.

[0016] As a further preferred scheme, the culture dish is detachably connected with the base through the lower section of the culture dish by screw threads.

[0017] As a further preferred scheme, the sealing ring is made of silica gel material.

[0018] The utility model has the following beneficial effects:

[0019] 1) The device can be used multiple times, and only the cell climbing sheet needs to be replaced, and the rest can withstand high-pressure steam sterilization.

[0020] 2) The cell climbing sheet is made of high-boron-silicon glass material and is more easily adhered to cells after surface treatment.

[0021] 3) The cell climbing sheet does not need to be fixed before use and is more convenient to take out.

[0022] 4) The extrusion force of the silica gel ring and the threaded structure ensures the sealing between the cell climbing sheet and the culture dish, so that the entire device is hollow while the sealing performance is guaranteed. The hollow feature can observe the cell growth state at any time through a microscope. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a perspective view of the cell climbing sheet device of the utility model;

[0024] Figure 2 It is a bottom view of the cell climbing sheet device of the utility model;

[0025] Figure 3 It is a front view of the cell climbing sheet device of the utility model;

[0026] Figure 4 It is a sectional view of the cell climbing sheet device of the utility model;

[0027] Figure 5 It is an explosion view of the cell climbing sheet device of the utility model. DETAILED DESCRIPTION

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Reference Figures 1-5 This utility model provides a cell climbing device.

[0030] Example 1

[0031] A cell climbing device includes a culture dish 10, a sealing ring 20, a cell climbing sheet 30, and a base 40 arranged sequentially from top to bottom.

[0032] The culture dish 10 has a hollow through-hole structure. The culture dish 10 includes an upper section 11 and a lower section 12. The upper section 11 and the lower section 12 are coaxially arranged. The cross-sectional area of ​​the upper section 11 is a, and the cross-sectional area of ​​the lower section 12 is b, satisfying the quantitative relationship: a < b.

[0033] The petri dish 10 is detachably connected to the base 40 via the lower section 12 of the petri dish.

[0034] The operation process achieved by existing devices and their auxiliary devices is relatively complex and prone to contamination.

[0035] In this embodiment of the present invention, a cell climbing device is provided, which can overcome the two disadvantages mentioned above, enabling cells to be cultured directly on the climbing sheet, and allowing the climbing sheet to be quickly removed for subsequent experiments after culture.

[0036] The specific working principle is as follows:

[0037] Assembly of S1 base 40 and petri dish 10

[0038] S11 Invert the culture dish 10 and place the sealing ring 20 and cell climbing sheet 30 in sequence;

[0039] S12 connects and fixes the base 40 to the petri dish 10;

[0040] S2 adds the cells to be cultured and the culture medium to the cell crawling slide 30 to complete the cell culture.

[0041] After S3 has been cultured, remove the excess liquid culture medium, unscrew culture dish 10, take out cell smear 30, and then proceed with subsequent experiments.

[0042] In this embodiment, since the culture dish 10 has a hollow through-structure, the cell growth status can be observed at any time during cell culture.

[0043] Example 2

[0044] In this embodiment:

[0045] The petri dish 10 is an axially penetrating hollow stepped cylinder, consisting of two coaxial cylinders, cylinder one and cylinder two.

[0046] The upper cylinder has a smaller diameter than the lower cylinder, and the two cylinders are connected by a radially abrupt transition surface to form a stepped transition, while the overall axial direction remains a continuous channel.

[0047] The structure of the more specific petri dish 10 is as follows:

[0048] Main body shape: It adopts a coaxial double cylindrical superimposed structure with a continuous central through hole in the axial direction;

[0049] Dimensional characteristics: Diameter D1 of the upper cylinder < Diameter D2 of the lower cylinder; Transition form: The joint surface of the upper and lower cylinders is abruptly connected in a vertical step shape;

[0050] Structural characteristics: There is a continuous cavity running through the entire structure along the axial direction.

[0051] In this embodiment, the specific operation steps can be referred to in Embodiment 1. The above-mentioned structural design makes the operation convenient and effectively prevents pollution.

[0052] Example 3

[0053] In this embodiment:

[0054] The petri dish 10 is detachably connected to the base 40 via the lower section 12 of the petri dish through threads.

[0055] In specific operation, in this embodiment:

[0056] The culture dish 10 has a hollow cylindrical structure. The upper part can be regarded as an ordinary culture dish, which is the cell culture functional area, while the inner side of the lower part has a screw-on cap.

[0057] The sealing ring 20 is inserted from under the culture dish 10, its diameter matching the central step of the dish, thus securing it. Next, the cell spreader 30 is inserted from under the dish 10; the sealing ring 20, through its interference fit at the edge, holds the cell spreader 30 in place. The base 40, also a hollow cylinder with threads, is screwed into the lower threaded portion of the culture dish 10, applying pressure to the sealing ring 20 and the cell spreader 30 to ensure a complete seal. The entire device is hollow, allowing for continuous observation of cell growth during culture.

[0058] The specific working principle is as follows: After assembly, it can be used directly. The cells to be prepared into a cell slide are directly transferred into the device and an appropriate amount of culture medium is added. After the culture is completed, the culture medium is aspirated, the screw is unscrewed and the cell slide is taken out, and then subsequent experiments are carried out.

[0059] Example 4

[0060] In this embodiment, the sealing ring 20 is made of silicone.

[0061] The choice of silicone material as the material for sealing ring 20 has the following advantages:

[0062] High elastic recovery rate: small permanent compression deformation and excellent sealing durability.

[0063] Low surface viscosity: Low coefficient of friction during dynamic sealing, suitable for sealing rotating shafts or reciprocating motion.

[0064] Electrical insulation: High resistivity

[0065] No plasticizer migration: No harmful substances are released during long-term use, avoiding media contamination.

[0066] In summary, the structures in embodiments 1 to 4 of this utility model have the following beneficial effects:

[0067] 1) Compared with the complex operations of fixing cell smears before use and removing them after use in the prior art, this invention optimizes these two steps, reducing experimental steps, saving time and reducing the risk of contamination.

[0068] 2) The device can be used multiple times; only the cell slides need to be replaced, and the rest can withstand autoclaving.

[0069] 3) The cell adhesion slide is made of high borosilicate glass and has undergone surface treatment, which makes it easier for cells to adhere to the wall.

[0070] 4) Cell slides do not require fixation before use, making them easier to remove;

[0071] 5) The compression force of the silicone ring and the threaded structure ensures the seal between the cell slide and the culture dish, so that the entire device is hollow while ensuring the sealing performance. The hollow feature allows for observation of cell growth status through a microscope at any time during use.

[0072] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cell-climbing device, characterized in that, The components, arranged from top to bottom, include a culture dish, a sealing ring, a cell spreader, and a base. The culture dish has a hollow through-hole structure. The culture dish includes an upper section and a lower section, which are coaxially arranged. The cross-sectional area of ​​the upper section is a, and the cross-sectional area of ​​the lower section is b, satisfying the quantitative relationship: a < b. The petri dish is detachably connected to the base via the lower section of the petri dish.

2. The cell-climbing device according to claim 1, characterized in that, The upper section of the petri dish is cylindrical, and the lower section of the petri dish is cylindrical. The petri dish is an axially penetrating hollow stepped cylinder, consisting of an upper cylinder and a lower cylinder arranged coaxially. The upper cylinder has a smaller diameter than the lower cylinder, and the two cylinders are connected by a radially abrupt transition surface to form a stepped transition, while the overall axial direction remains a continuous channel.

3. The cell-climbing device according to claim 1, characterized in that, The petri dish is a one-piece molded structure.

4. The cell spreading device according to any one of claims 1 or 2, characterized in that, The petri dish is detachably connected to the base via threads at the bottom.

5. The cell spreading device according to any one of claims 1, 2, or 4, characterized in that, The sealing ring is made of silicone.

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