Pulmonary alveolar lavage fluid collecting device for rats and mice
The design of the reverse fork locking structure and smooth needle simplifies the bronchoalveolar lavage fluid collection process, solves the problems of complex operation and sample contamination, and achieves efficient and safe sample recovery.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-03-24
AI Technical Summary
Existing technologies for collecting bronchoalveolar lavage fluid involve complex and time-consuming procedures, and the ligation is not secure, leading to problems such as sample contamination and low recovery rates.
The tracheal clamp with its reverse fork locking structure and smooth needle design simplifies the operation process, ensures no leakage of irrigation fluid, and is suitable for laboratory animals of different sizes.
It improves operational efficiency, increases sample recovery volume and quality, reduces the risk of sample contamination, and adapts to the needs of laboratory animals of different sizes.
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Figure CN224023590U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of auxiliary equipment for biomedical research, specifically to a device for collecting bronchoalveolar lavage fluid from mice and rats. Background Technology
[0002] In mouse and rat experiments, the collection of bronchoalveolar lavage fluid (BALF) is a routine technique used to analyze changes in airway inflammation, assess immune responses, detect pathogens, and even monitor lung damage and study disease models. Through cytological, biochemical, and molecular biological analysis of BALF, researchers can gain a deeper understanding of lung pathology, providing a scientific basis for the diagnosis and treatment of respiratory diseases.
[0003] In current technical practice, the conventional method for collecting bronchoalveolar lavage fluid (BALF) involves using pre-cooled buffer solution as the lavage fluid, surgically incising the neck skin and muscles near the trachea to expose the trachea, placing sutures under the trachea, inserting the endotracheal tube, ligating it with sutures, and then collecting the sample after lavage. However, this conventional method has several problems: First, the procedure is complex and time-consuming, requiring a high level of skill and precision; second, the sutures may not be secure enough, causing the lavage fluid to leak out through the gaps, which could not only contaminate the sample but also affect the sample recovery rate, thus impacting the efficiency and reliability of BLF collection.
[0004] Given the aforementioned limitations, a novel irrigation device is needed for laboratory animal surgery that simplifies the bronchoalveolar lavage fluid collection process and improves experimental efficiency and safety. Utility Model Content
[0005] To address the aforementioned technical problems, this invention aims to provide a simple, high-success-rate, and thorough bronchoalveolar lavage fluid (BALF) collection device for mice and rats, thereby achieving more efficient and safer BALF collection.
[0006] To achieve the above objectives, this utility model provides:
[0007] A device for collecting bronchoalveolar lavage fluid from mice and rats, comprising:
[0008] Irrigation needle (100), the irrigation needle (100) includes a needle (101), a catheter (102) and a tube seat (103) connected sequentially from bottom to top, wherein the outer surface of the needle (101) is smooth to prevent scratching the tracheal wall;
[0009] A tracheal clamp (200) having a reverse fork locking structure, the tracheal clamp (200) being matched with the catheter (102) of the irrigation needle (100) for securely clamping the catheter (102).
[0010] Compared with the prior art, the utility model has the advantages of:
[0011] 1. Simplify the operation process: simplify the ligation step, reduce the operation time, thereby improve the efficiency.
[0012] 2. Improve sample quality: prevent the lavage fluid from leaking out of the gap through the improved structure, which can increase the recovery amount of the sample and prevent the sample from being contaminated.
[0013] 3. Adapt to different sizes of experimental animals: considering different sizes of experimental animals such as rats and mice, a plurality of experimental collection devices of different sizes and structures are provided to meet the needs of different experimental scenes. BRIEF DESCRIPTION OF DRAWINGS
[0014] Figure 1 It is the structure schematic view of the alveolar lavage fluid collection device for rats and mice of the utility model embodiment 1-5.
[0015] The reference signs are as follows:
[0016] 100-lavaging needle; 101-needle head; 102-catheter; 103-tube seat;
[0017] 200-tracheal clamp; 201-clamp mouth; 202-handle. DETAILED DESCRIPTION
[0018] The implementation scheme of the utility model will be described in detail in combination with the embodiments below, but the person skilled in the art needs to understand that the following embodiments are only used to illustrate the utility model, and should not be regarded as limiting the scope of the utility model. The specific conditions are not marked in the embodiments, and are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used are not marked by the manufacturer, and are conventional products that can be purchased in the market.
[0019] Embodiment 1
[0020] The embodiment provides an alveolar lavage fluid collection device for rats and mice, which comprises:
[0021] The lavaging needle 100 comprises a needle head 101, a catheter 102 and a tube seat 103 connected in turn from bottom to top, wherein the outer surface of the needle head 101 is smooth, which is used to prevent scratching the tracheal wall, and the tube seat 103 can be used to connect a disposable syringe;
[0022] The tracheal clamp 200 has a reverse fork lock clamp structure, which matches the catheter 102 of the lavaging needle 100, and is used to clamp the catheter 102 stably.
[0023] In traditional alveolar lavage fluid collection methods, sutures are used to ligate the trachea to secure the catheter, which is a complex and time-consuming process. The tracheal clamp in this embodiment uses a reverse fork lock clamp structure to directly clamp the trachea, eliminating the need for ligation, thereby simplifying the surgical procedure, reducing the operation time, and improving efficiency.
[0024] Because the ligation with sutures in traditional methods may not be secure enough, causing lavage fluid to leak from the gap, resulting in sample contamination or low recovery. The design of the tracheal clamp in this embodiment ensures the tight closure between the lavage needle and the trachea, preventing the leakage of lavage fluid, ensuring the integrity and purity of the sample, increasing the sample recovery amount, and reducing the pollution risk caused by leakage.
[0025] In one embodiment, when in use, the lavage needle 100 is bent 30°-35°, preferably 30°, on the side close to the tube seat 103, to facilitate the connection of the syringe after intubation. This curved design makes it easier to adjust the angle after the lavage needle is inserted into the trachea, so that the disposable syringe can be connected smoothly without worrying about the inconvenience caused by the angle or additional stress and damage to the animal. In addition, such a design also helps the experimenter to operate more flexibly in the limited operating space, improving the convenience and efficiency of the surgical process.
[0026] In one embodiment, the reverse fork lock clamp structure includes a clamp mouth 201 and a handle 202, and the clamp mouth 201 and the handle 202 are respectively arranged on both sides of the reverse fork lock clamp structure.
[0027] In one embodiment, when the tracheal clamp is closed, the reverse fork lock clamp structure will automatically lock through a structural mechanism, without the need for additional external force to fix the tracheal clamp, such as using the gripping force of the hand to make the clamp mouth of the tracheal clamp tight, thereby ensuring that the lavage needle and the trachea are tightly closed without leakage under the action of external force. For example, once the clamp mouth is closed to a certain extent, the locking mechanism is triggered, keeping the clamp closed without easily opening. To achieve the above locking mechanism, the middle of the reverse fork lock clamp structure can be composed of a series of teeth (ratchet) and a component (pawl) that can slide and be locked into these teeth. When the two halves of the tracheal clamp are closed, the pawl will slide along the ratchet until the desired closure degree is reached. At this time, the pawl will automatically lock into a tooth of the ratchet, preventing the clamp from accidentally opening. To achieve the above locking mechanism, a spring can also be used to exert pressure, so that when the clamp mouth is closed to a certain position, the force of the spring will make the latch component in the tracheal clamp enter the locked state. For example, small pins or hooks are arranged inside the tracheal clamp, which are ejected by the force of the spring when the clamp is closed to the predetermined position, and are docked with the opposite recess or hole, thereby achieving locking.
[0028] In another embodiment, the reverse fork lock structure can only exert pressure through a spring, such as installing a spring inside the tracheal clamp, which provides a force to rotate the reverse fork lock structure inward in the rotating direction. After the user opens the clamp mouth to the appropriate clamping angle, the reverse fork lock structure can automatically clamp under the action of the spring recovery force.
[0029] In another embodiment, the reverse fork lock structure can only be provided with a rotating shaft in the middle of the structure, so that the reverse fork lock structure can be freely rotated. When the clamp mouth fixes the object (such as the trachea of a mouse or rat), the user can use a little more force to fix the object.
[0030] In one embodiment, the clamp mouth 201 is provided with a semicircular groove structure, and the two sides of the clamp mouth 201 form a complete circle when closed. When the tracheal clamp is closed to form a complete circle, it can provide uniform pressure distribution on the entire circumference, thereby ensuring the close contact between the lavage needle and the trachea. This tight seal prevents lavage fluid from leaking from the interface, avoids sample contamination or loss, and improves sample recovery rate and experimental result reliability. The tracheal wall is relatively soft and easily damaged. By designing the clamp mouth to be circular, the pressure can be dispersed as much as possible during clamping, reducing the risk of high local pressure causing damage to the tracheal wall and protecting the animal's airway structure. In addition, the circular design is based on the cross-sectional shape of most catheters, including the lavage needle in this embodiment. Since the catheter of the lavage needle is usually circular, using a circular tracheal clamp can better match the shape of the catheter, ensuring that there is no gap between the two, further enhancing the sealing performance.
[0031] In one embodiment, the inner groove wall of the semicircular groove structure of the clamp mouth 201 on both sides is provided with a silicone sheet, which has a certain elasticity and can better fit the trachea. In order to better fit the trachea, silicone sheets are also arranged inside the clamp mouth, which not only increases the friction to help maintain the closed state, but also the softness of the silicone can adapt to the shape of the trachea, reducing the pressure and potential damage to the tissue.
[0032] In one embodiment, the needle 101 has a water droplet-like structure with a narrow upper head and a wide lower head, and the outer surface of the needle 101 is smooth, which can avoid scratching the tracheal wall and reduce the possibility of red blood cells and other impurities mixing into the sample, further improving the quality of the sample.
[0033] In one embodiment, the lavage needle 100 and the tracheal clamp 200 are made of 304 stainless steel, which has good corrosion resistance and formability, and is easy to clean. The lavage needle and the tracheal clamp are made of 304 stainless steel, which has good corrosion resistance and formability, and is easy to clean, ensuring long-term use and hygiene standards of the equipment, and indirectly promoting the improvement of sample quality.
[0034] In this embodiment, the diameter of the catheter 102 of the lavage needle 100 is 0.7mm, and the bending diameter of the tracheal clamp 200 is 0.9mm.
[0035] Embodiment 2
[0036] This embodiment provides a lung alveolar lavage fluid collection device for rats and mice, which is different from embodiment 1 in that the diameter of the catheter 102 of the lavage needle 100 is 0.9mm, and the bending diameter of the tracheal clamp 200 is 1.16mm, and the rest is the same.
[0037] In order to adapt to experimental animals of different sizes, the utility model provides a plurality of models, and the sizes of the lavage needle and the tracheal clamp of each model are carefully designed to match the trachea of a specific size.
[0038] Embodiment 3
[0039] This embodiment provides a lung alveolar lavage fluid collection device for rats and mice, which is different from embodiment 1 in that the diameter of the catheter 102 of the lavage needle 100 is 1.25mm, and the bending diameter of the tracheal clamp 200 is 1.6mm, and the rest is the same.
[0040] In order to adapt to experimental animals of different sizes, the utility model provides a plurality of models, and the sizes of the lavage needle and the tracheal clamp of each model are carefully designed to match the trachea of a specific size.
[0041] Embodiment 4
[0042] This embodiment provides a lung alveolar lavage fluid collection device for rats and mice, which is different from embodiment 1 in that the diameter of the catheter 102 of the lavage needle 100 is 1.6mm, and the bending diameter of the tracheal clamp 200 is 2.1mm, and the rest is the same.
[0043] In order to adapt to experimental animals of different sizes, the utility model provides a plurality of models, and the sizes of the lavage needle and the tracheal clamp of each model are carefully designed to match the trachea of a specific size.
[0044] Embodiment 5
[0045] The embodiment provides a kind of alveolar lavage fluid collection device for mouse, different from the embodiment 1, the diameter of the catheter 102 of the lavage needle 100 is 2.5mm, the bending diameter of the tracheal clamp 200 is 3.0mm, the same remains.
[0046] To adapt to different sizes of experimental animals, the present application provides a variety of models, and the size of the lavage needle and tracheal clamp of each model is carefully designed to match the trachea of a specific size.
[0047] Mouse bronchoalveolar lavage method
[0048] The experimental steps are as follows:
[0049] 1. First, the mouse is properly anesthetized.
[0050] 2. Fix the mouse: place the mouse on its back, fix the limbs with medical tape, and fix the head by hanging the teeth on a tight line.
[0051] 3. Dissect and expose the trachea: use scissors to open the mouse's chest, separate the muscle tissue around the trachea, and expose the trachea.
[0052] 4. Intubation: cut a small opening near the upper part of the trachea close to the mouse's head, select the appropriate alveolar lavage fluid collection device according to the alveolar lavage fluid collection device for mouse described in embodiments 1-5, insert the lavage needle 100 into the upper end of the trachea, and clamp it with the tracheal clamp 200.
[0053] 5. Prepare the lavage fluid: prepare physiological saline containing 1% PBS (Phosphate Buffered Saline) in advance, and store it on ice for use in alveolar lavage.
[0054] 6. Lavage: use a 1mL syringe to remove the needle and insert it into the lavage needle to lavage the mouse's lungs. Extract 0.3mL of pre-cooled PBS with the syringe, slowly lavage the left lung, and after three strokes, slowly withdraw the liquid and massage the mouse's chest at the same time. The obtained lavage fluid is placed in a 1.5mL EP tube (Eppendorf tube), and stored on ice. Each mouse is lavaged 4 times, and the total amount of recovered lavage fluid is greater than or equal to 1.0mL.
[0055] 7. Lavage fluid treatment: after lavage, place the lavage fluid test tube on ice or in the refrigerator, centrifuge the lavage fluid, and separate the cell components and supernatant for subsequent detection.
[0056] 8. Cell counting and analysis: cell components can be analyzed by total cell or white blood cell counting. The number and activity of cells in lavage fluid obtained by different methods may be different.
[0057] 9. Subsequent experiments: supernatant in lavage fluid can be used for biochemical detection and cytokine analysis, while cell precipitate can be used for flow cytometry analysis.
[0058] The above merely describes a preferred embodiment of the present application, but the protection scope of the present application is not limited thereto, any skilled person in the art, according to the technical scheme and concept of the present application, makes equivalent replacement or change within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.
Claims
1. A device for collecting bronchoalveolar lavage fluid from rats and mice, characterized in that, include: Irrigation needle (100), the irrigation needle (100) includes a needle (101), a catheter (102) and a tube seat (103) connected sequentially from bottom to top, wherein the outer surface of the needle (101) is smooth to prevent scratching the tracheal wall; A tracheal clamp (200) having a reverse fork locking structure, the tracheal clamp (200) being matched with the catheter (102) of the irrigation needle (100) for securely clamping the catheter (102).
2. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 1, characterized in that, When in use, the irrigation needle (100) is bent at 30°-35° on the side near the tube seat (103).
3. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 1, characterized in that, The reverse fork lock structure includes a clamp (201) and a handle (202), with the clamp (201) and the handle (202) respectively located on both sides of the reverse fork lock structure.
4. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 3, characterized in that, The clamp (201) has a semi-circular groove structure, and the clamps (201) on both sides form a complete circle when closed.
5. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 1, characterized in that, The inner walls of the semi-circular groove structure of the clamps (201) on both sides are provided with silicone sheets.
6. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 1, characterized in that, The needle (101) has a teardrop-shaped structure that is narrow at the top and wide at the bottom.
7. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 1, characterized in that, The irrigation needle (100) and the tracheal clamp (200) are both made of 304 stainless steel.
8. The bronchoalveolar lavage fluid collection device for mice and rats according to any one of claims 1-7, characterized in that, The diameter of the catheter (102) of the irrigation needle (100) is 0.7mm-2.5mm, and the bending diameter of the tracheal clamp (200) is 0.9mm-3.0mm.
9. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 8, characterized in that, The diameter of the catheter (102) of the irrigation needle (100) is 0.7 mm, and the bending diameter of the tracheal clamp (200) is 0.9 mm.
10. The bronchoalveolar lavage fluid collection device for mice and rats according to claim 8, characterized in that, The diameter of the catheter (102) of the irrigation needle (100) is 0.9 mm, and the bending diameter of the tracheal clamp (200) is 1.16 mm. Alternatively, the diameter of the catheter (102) of the irrigation needle (100) is 1.25 mm, and the bending diameter of the tracheal clamp (200) is 1.6 mm; Alternatively, the diameter of the catheter (102) of the irrigation needle (100) is 1.6 mm, and the bending diameter of the tracheal clamp (200) is 2.1 mm; Alternatively, the diameter of the catheter (102) of the irrigation needle (100) is 2.5 mm, and the bending diameter of the tracheal clamp (200) is 3.0 mm.