Molecular molecular sieve collagen desalting and concentrating equipment

Abstract

The utility model discloses molecular sieve separation collagen desalting and concentrating equipment which comprises a supporting frame, a screening tank is installed on the outer wall of the top of the supporting frame, a concentrated solution outlet pipe is installed on the outer wall of one side of the screening tank, a pressure gauge is installed on the outer wall of the top of the screening tank, and a plurality of connecting pipes are connected to the outer wall of the screening tank. The ends, away from the screening tank, of the multiple connecting pipes are connected with a pressure pump, a circulating pump, a permeate collecting tank and an infusion pump correspondingly, and valves are installed at the ends, close to the screening tank, of the concentrated solution outlet pipe, the pressure pump and the circulating pump correspondingly. The filtering mechanism comprises a stock solution tank installed on the outer wall of one side of the supporting frame, and the outer wall of one side of the stock solution tank is connected with a liquid conveying pipe. The molecular sieve separation collagen desalting and concentrating equipment disclosed by the utility model has the effects of removing impurities in a solution, improving the separation effect and avoiding local concentration difference in the tank body.

Description

TECHNICAL FIELD

[0001] The utility model relates to chemical separation technical field especially relates to a molecular sieve divides collagen protein desalination concentration equipment. BACKGROUND

[0002] In the field of bioengineering and food industry, the extraction and purification of collagen protein are crucial. High-quality collagen protein has a wide application prospect in the pharmaceutical, cosmetic, health care product and other industries. As the key equipment in the process of collagen protein extraction and purification, the performance of the molecular sieve collagen protein desalination concentration equipment directly affects the quality and production efficiency of collagen protein products. With the continuous progress of science and technology, the market requires higher quality and purity of collagen protein products.

[0003] However, the traditional molecular sieve collagen protein desalination concentration equipment does not sufficiently remove impurities from the collagen protein solution entering the molecular sieve membrane, which makes some large particle impurities, colloidal substances or denatured proteins in the solution easily adhere to the surface of the molecular sieve membrane, thereby blocking the membrane pores, affecting the separation effect and the running stability of the equipment.

[0004] For example, in the collagen protein production process, the molecular sieve collagen protein desalination concentration equipment used lacks a perfect pretreatment system. When processing collagen protein solution, large particle impurities and colloidal substances in the solution enter the molecular sieve membrane with the solution. Over time, these impurities gradually adhere to the membrane surface, causing membrane pore blockage and reducing the separation effect. UTILITY MODEL CONTENT

[0005] The utility model discloses a molecular sieve collagen protein desalination concentration equipment, which aims to solve the technical problem that the traditional molecular sieve collagen protein desalination concentration equipment does not sufficiently remove impurities from the collagen protein solution entering the molecular sieve membrane, which makes some large particle impurities, colloidal substances or denatured proteins in the solution easily adhere to the surface of the molecular sieve membrane, thereby blocking the membrane pores, affecting the separation effect and the running stability of the equipment.

[0006] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0007] The application discloses a molecular sieve collagen desalination and concentration device, which comprises a support frame, a screening tank is installed on the top outer wall of the support frame, a concentrated liquid outlet pipe is installed on one side outer wall of the screening tank, a pressure gauge is installed on the top outer wall of the screening tank, a plurality of connecting pipes are connected to the outer wall of the screening tank, and the far ends of the connecting pipes are respectively connected with a pressure pump, a circulating pump, a permeated liquid collecting tank and a liquid pumping pump; the near end of the concentrated liquid outlet pipe, the pressure pump and the circulating pump are all provided with a valve; the device further comprises a filtering mechanism, which comprises an original liquid tank installed on one side outer wall of the support frame; one side outer wall of the original liquid tank is connected with a liquid feeding pipe; one end of the liquid feeding pipe is connected with a connecting plate; a sealing gasket is fixed to one side inner wall of the connecting plate away from the liquid feeding pipe; one side outer wall of the two connecting plates is connected with a same filter cartridge; a connecting cylinder is installed on the inner wall of the filter cartridge; the two side outer walls of the connecting cylinder are symmetrically provided with fixing blocks; two coarse filtering plates are connected to the end of the connecting cylinder close to the liquid feeding pipe; and two fine filtering plates are connected to the end of the connecting cylinder away from the liquid feeding pipe; and a stirring mechanism is arranged on the top outer wall of the screening tank.

[0008] The support frame provides a solid support foundation for the whole filtering mechanism; the original liquid in the original liquid tank is connected with the filter cartridge through the liquid feeding pipe, so that the original liquid can flow into the filter cartridge from the liquid feeding pipe; the sealing gaskets are arranged on the two side outer walls of the filter cartridge and the two connecting plates and the side outer wall of the connecting plate in close contact with the two side outer walls, so that the sealing property of the connecting part of the connecting plate and the filter cartridge is improved, and the leakage of the original liquid in the conveying process is avoided; the connecting cylinder is arranged in the filter cartridge through the fixing blocks, and the two coarse filtering plates and the fine filtering plates are arranged on the inner wall of the connecting cylinder, so that the coarse filtering plates and the fine filtering plates are supported and fixed; the original liquid is preliminarily filtered through the coarse filtering plates, the impurities with large particles are intercepted, and the filtering pressure of the subsequent fine filtering plates is reduced; the liquid filtered through the coarse filtering plates is finely filtered through the fine filtering plates, the impurities with small particles are removed, and the purity of the filtered liquid is further improved; and the collagen solution is fully impurity-removed, so that the membrane holes are prevented from being blocked due to the adhesion of some large-particle impurities and colloidal substances in the solution to the surface of the molecular sieve membrane.

[0009] In a preferred scheme, the stirring mechanism comprises a motor installed on the top outer wall of the screening tank, a stirring paddle is installed at the output end of the motor, a chromatographic column is installed on the inner wall of the screening tank, and a plurality of flow guides are installed on the inner wall of the screening tank.

[0010] The motor is started to drive the stirring paddle to rotate, thereby fully stirring the liquid in the screening tank, at this time, the liquid components in the tank are mixed more uniformly, thereby avoiding local concentration differences, meanwhile, the multiple guide plates installed on the inner wall of the screening tank play a role of guiding the flow direction of the liquid, the guide plates can make the liquid in the tank form an orderly flow path, avoiding turbulence or dead angles of the liquid, when the stirred liquid flows through the chromatography column, at this time, the separation membrane with a specific pore size is arranged in the chromatography column, thereby separating different components in the mixed liquid, achieving the purposes of screening and purification.

[0011] As can be seen from the above, the support frame is provided with the screening tank on the top outer wall, the concentrated liquid outlet pipe is installed on one side outer wall of the screening tank, the pressure gauge is installed on the top outer wall of the screening tank, the multiple connecting pipes are connected to the outer wall of the screening tank, the one ends of the multiple connecting pipes away from the screening tank are respectively connected with the pressure pump, the circulating pump, the permeate collection tank and the liquid pumping pump, the one ends of the concentrated liquid outlet pipe, the pressure pump and the circulating pump close to the screening tank are all provided with the valve, and the filtering mechanism is further arranged, which comprises the raw liquid tank installed on one side outer wall of the support frame, the raw liquid tank is connected with the liquid inlet pipe, the one end of the liquid inlet pipe is connected with the connecting plate, the sealing pad is fixed on the one side inner wall of the connecting plate away from the liquid inlet pipe, the same filter cartridge is connected to the one side outer wall of the two connecting plates, the connecting cylinder is installed on the inner wall of the filter cartridge, the fixing blocks are symmetrically arranged on the two side outer walls of the connecting cylinder, the two coarse filter plates are connected to the one end of the connecting cylinder close to the liquid inlet pipe, and the two fine filter plates are connected to the one end of the connecting cylinder away from the liquid inlet pipe. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 The overall structure schematic view of the molecular screening collagen protein desalination and concentration equipment is provided.

[0013] Figure 2 The filter cartridge structure schematic view of the molecular screening collagen protein desalination and concentration equipment is provided.

[0014] Figure 3 The filtering mechanism schematic view of the molecular screening collagen protein desalination and concentration equipment is provided.

[0015] Figure 4 The stirring mechanism schematic view of the molecular screening collagen protein desalination and concentration equipment is provided.

[0016] Figure 5The utility model discloses a kind of molecular sieve collagen protein desalination concentration equipment, which is mainly applied to the scene that traditional molecular sieve collagen protein desalination concentration equipment does not carry out insufficient impurity removal treatment to collagen protein solution entering molecular sieve membrane, which makes some large-particle impurities, colloidal substances or denatured proteins in solution easily adhere to the surface of molecular sieve membrane, and then block membrane holes, affect separation effect and the running stability of equipment. Figure 3

[0017] In the drawings: 1, support frame; 2, stock solution tank; 3, filter cartridge; 4, screening tank; 5, concentrated liquid outlet pipe; 6, pressure pump; 7, circulating pump; 8, permeate collection tank; 9, liquid pump; 10, connecting plate; 11, bolt; 12, nut; 13, coarse filter plate; 14, fine filter plate; 15, gasket; 16, chromatography column; 17, stirring paddle; 18, guide plate; 19, motor; 20, fixed block; 21, connecting cylinder. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all embodiments. The components of the embodiments of the present application described and indicated in the drawings can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0019] The molecular sieve collagen protein desalination concentration equipment disclosed by the utility model is mainly applied to the scene that traditional molecular sieve collagen protein desalination concentration equipment does not carry out insufficient impurity removal treatment to collagen protein solution entering molecular sieve membrane, which makes some large-particle impurities, colloidal substances or denatured proteins in solution easily adhere to the surface of molecular sieve membrane, and then block membrane holes, affect separation effect and the running stability of equipment.

[0020] Reference is made to Figure 1 , Figure 2 , Figure 3 and Figure 5 ​The utility model provides a kind of molecular sieve collagen desalination concentration equipment, including support frame 1, the top outer wall of support frame 1 is equipped with screening tank 4, the outer wall of one side of screening tank 4 is equipped with concentrated liquid outlet pipe 5, the top outer wall of screening tank 4 is equipped with pressure gauge, the outer wall of screening tank 4 is connected with multiple connecting pipes, the end of multiple connecting pipes away from screening tank 4 is respectively connected with pressure pump 6, circulating pump 7, permeate collection tank 8 and liquid pumping pump 9, concentrated liquid outlet pipe 5, pressure pump 6 and circulating pump 7 the end close to screening tank 4 is equipped with valve, and it further includes: filtering mechanism: filtering mechanism includes raw liquid tank 2 installed in the outer wall of one side of support frame 1, the outer wall of one side of raw liquid tank 2 is connected with infusion tube, the end of infusion tube is connected with connecting plate 10, the inner wall of one side of connecting plate 10 away from infusion tube is fixed with sealing gasket 15, the outer wall of one side of two connecting plates 10 is connected with the same filter cartridge 3, the inner wall of filter cartridge 3 is equipped with connecting cylinder 21, the outer wall of both sides of connecting cylinder 21 is symmetrically distributed with fixed block 20, the end of connecting cylinder 21 close to infusion tube is connected with two coarse filter plates 13, the end of connecting cylinder 21 away from infusion tube is connected with two fine filter plates 14;Stirring mechanism: the stirring mechanism is located on the top outer wall of screening tank 4.

[0021] Support frame 1 provides solid support foundation for the whole filtering mechanism in the scheme, the raw liquid to be filtered in raw liquid tank 2 is connected with filter cartridge 3 through infusion tube, so that the raw liquid flows from infusion tube into filter cartridge 3, at this time, sealing gasket 15 is installed on the outer wall of both sides of filter cartridge 3 and the outer wall of one side of two connecting plates 10, so that the sealing property of the connecting part of connecting plate 10 and filter cartridge 3 is enhanced, to prevent leakage of raw liquid during transportation, at this time, connecting cylinder 21 is installed in the inside of filter cartridge 3 by using fixed block 20, and two coarse filter plates 13 and fine filter plates 14 are installed in the inner wall of connecting cylinder 21, so as to support and fix coarse filter plates 13 and fine filter plates 14, at this time, the flowing raw liquid is preliminarily filtered through coarse filter plates 13, to intercept larger impurity particles and reduce the filtering pressure of subsequent fine filter plates 14, at this time, the liquid filtered through coarse filter plates 13 is finely filtered through fine filter plates 14, to remove small impurity particles and further improve the purity of filtrate, at this time, the collagen solution is fully decontaminated, so as to avoid that some large-particle impurities and colloidal substances in the solution adhere to the surface of molecular sieve membrane and cause the membrane holes to be blocked.

[0022] The outer wall of both sides of two connecting plates 10 and filter cartridge 3 is equipped with lifting lug, the outer wall of both sides of two connecting plates 10 is connected with bolt 11, the outer wall of one side of bolt 11 away from connecting plate 10 is connected with nut 12, the threads of bolt 11 and nut 12 are screwed or loosened, so as to disassemble and fix connecting cylinder 21.

[0023] In particular, the sealing gasket 15 is fixed at both ends of the filter cartridge 3, and the sealing gasket 15 is installed on the filter cartridge 3 and the connecting plate 10 to play a sealing role.

[0024] In particular, the filter hole in the coarse filter plate 13 is slightly larger than the filter hole in the fine filter plate 14, and at the initial stage of the filtration process, the coarse filter plate 13 can quickly remove the impurities with a large volume in the raw solution by virtue of the larger filter hole. After the coarse filter plate 13 completes the preliminary filtration, the fine filter plate 14 can intercept impurities with a smaller particle size due to the smaller filter hole.

[0025] With reference to Figure 1 , Figure 2 and Figure 4 , in a preferred embodiment, the stirring mechanism includes a motor 19 installed on the outer wall of the top of the screening tank 4, the output end of the motor 19 is provided with a stirring paddle 17, the inner wall of the screening tank 4 is provided with a chromatography column 16, and the inner wall of the screening tank 4 is provided with a plurality of flow guides 18.

[0026] In this scheme, the motor 19 is started to drive the stirring paddle 17 to rotate, thereby fully stirring the liquid in the screening tank 4. At this time, the composition of the liquid in the tank is mixed more uniformly, thereby avoiding local concentration differences. Meanwhile, the plurality of flow guides 18 installed on the inner wall of the screening tank 4 play a role in guiding the flow direction of the liquid. The flow guides 18 can make the liquid in the tank form an orderly flow path, avoiding turbulence or dead angles of the liquid. When the stirred liquid flows through the chromatography column 16, a separation membrane with a specific pore size is arranged in the chromatography column 16, thereby separating different components in the mixed liquid, achieving the purposes of screening and purification.

[0027] The inside of the chromatography column 16 is composed of a separation membrane with a specific pore size, and the small holes on the outer wall of the chromatography column 16 can allow the liquid that has passed through the membrane to pass smoothly. By selecting a membrane with a suitable pore size, small molecular salt ions and water can pass through the membrane, while large molecular collagen proteins are intercepted, thereby achieving the effects of desalination and concentration.

[0028] In particular, the inner wall of the screening tank 4 is provided with a liquid accumulation space between the outer wall of the chromatography column 16 and the bottom outer wall of the chromatography column 16, respectively. The liquid accumulation space between the inner wall of the screening tank 4 and the outer wall of the chromatography column 16 is used to collect the permeate, and the liquid accumulation space between the inner wall of the screening tank 4 and the bottom outer wall of the chromatography column 16 is used to collect the concentrated solution.

[0029] Working principle: in use, first, the collagen stock solution to be treated is stored in the stock solution tank 2, at the same time, the stock solution tank 2 is connected with the connecting plate 10 through the infusion tube, at this time, the stock solution is smoothly transported from the stock solution tank 2 to the filter cartridge 3 by starting the liquid pumping pump 9, at this time, the connecting cylinder 21 is installed in the inner wall of the filter cartridge 3 through the fixing block 20, at this time, the filter cartridge 3 is connected with the two connecting plates 10 through the bolts 11 and the nuts 12, so as to achieve the disassembly and fixation of the connecting cylinder 21, at the same time, the sealing gaskets 15 are installed at the connecting positions of the two, so as to achieve the effect of preventing the stock solution from leaking, when the stock solution enters the filter cartridge 3, it is preliminarily filtered through the two coarse filter plates 13, and the larger impurity particles, such as undissolved solid particles, large size colloids and the like, are intercepted, then, the liquid filtered coarsely continues to flow to the two fine filter plates 14, the fine filter plates 14 finely filter the liquid, and remove the small impurity particles, such as small colloidal particles, denatured proteins and the like, so as to further improve the purity of the filtrate and provide high-quality raw material liquid for subsequent molecular sieving, at this time, the collagen solution pretreated by filtering is connected with the connecting pipe, and enters the sieving tank 4 under the action of the pressure pump 6, at this time, the motor 19 installed on the top outer wall of the sieving tank 4 is started, so as to drive the stirring paddle 17 to rotate, and then the solution in the tank is fully stirred, so that the solution components are uniformly mixed, and local concentration difference is avoided, at the same time, the flow guide plate 18 guides the liquid to form an orderly flow path, so as to prevent turbulence or dead angle, at this time, when the uniformly stirred liquid flows through the chromatography column 16, the chromatography column 16 is internally composed of a separation membrane with a specific pore size, and small holes are equidistantly distributed on the outer wall thereof, at this time, the small molecules of salt ions and water can pass through the separation membrane with a specific pore size, and then flow out through the small holes on the outer wall of the chromatography column 16, and enter the liquid accumulation space between the inner wall of the sieving tank 4 and the outer wall of the chromatography column 16, and then are transported to the permeate collection tank 8, so as to complete the desalination process, while the macromolecular collagen is intercepted, and as the liquid continuously enters, the liquid accumulation space between the inner wall of the sieving tank 4 and the bottom outer wall of the chromatography column 16 is gradually concentrated, at this time, the concentrated collagen solution is sent to the inlet again by starting the circulating pump 7, so as to improve the sieving effect, at this time, the valve on the top of the concentrated liquid outlet pipe 5 is opened, so as to discharge the concentrated collagen solution from the sieving tank 4, at the same time, the pressure gauge installed on the top outer wall of the sieving tank 4 monitors the pressure in the tank in real time, so as to ensure the safe and stable operation of the equipment.

[0030] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited to this. The replacement can be the replacement of part of the structure, device, method step, or the complete technical solution. According to the technical solution and the utility model concept of the present application, equivalent replacement or change should be covered within the protection scope of the present application.

Claims

1. A molecular sieving collagen desalting and concentration device, comprising a support frame (1), a sieving tank (4) installed on the top outer wall of the support frame (1), a concentrate outlet pipe (5) installed on one side outer wall of the sieving tank (4), a pressure gauge installed on the top outer wall of the sieving tank (4), and multiple connecting pipes connected to the outer wall of the sieving tank (4). The ends of the multiple connecting pipes away from the sieving tank (4) are respectively connected to a pressure pump (6), a circulation pump (7), a permeate collection tank (8), and a pumping pump (9). Valves are installed at the ends of the concentrate outlet pipe (5), the pressure pump (6), and the circulation pump (7) near the sieving tank (4), characterized in that... Also includes: Filtration mechanism: The filtration mechanism includes a raw liquid tank (2) installed on one side of the outer wall of the support frame (1). An infusion tube is connected to one side of the outer wall of the raw liquid tank (2). A connecting plate (10) is connected to one end of the infusion tube. A sealing gasket (15) is fixed to the inner wall of the connecting plate (10) away from the infusion tube. The same filter cylinder (3) is connected to one side of the outer wall of both connecting plates (10). A connecting cylinder (21) is installed on the inner wall of the filter cylinder (3). Fixing blocks (20) are symmetrically distributed on both sides of the outer wall of the connecting cylinder (21). Two coarse filter plates (13) are connected to the end of the connecting cylinder (21) near the infusion tube. Two fine filter plates (14) are connected to the end of the connecting cylinder (21) away from the infusion tube. Stirring mechanism: The stirring mechanism is located on the top outer wall of the screening tank (4).

2. The molecular sieving collagen desalting and concentration equipment according to claim 1, characterized in that, Lifting lugs are installed on both sides of the outer walls of the two connecting plates (10) and the filter cartridge (3). Bolts (11) are connected to both sides of the outer walls of the two connecting plates (10). Nuts (12) are connected to the outer wall of the bolts (11) away from the connecting plates (10).

3. The molecular sieving collagen desalting and concentration equipment according to claim 1, characterized in that, The sealing gasket (15) is fixed at both ends of the filter cartridge (3).

4. The molecular sieving collagen desalting and concentration equipment according to claim 1, characterized in that, The filter holes inside the coarse filter plate (13) are slightly larger than the filter holes inside the fine filter plate (14).

5. The molecular sieving collagen desalting and concentration equipment according to claim 1, characterized in that, The stirring mechanism includes a motor (19) installed on the top outer wall of the screening tank (4), a stirring paddle (17) installed at the output end of the motor (19), a chromatography column (16) installed on the inner wall of the screening tank (4), and multiple guide plates (18) installed on the inner wall of the screening tank (4).

6. The molecular sieving collagen desalting and concentration equipment according to claim 5, characterized in that, The interior of the chromatography column (16) is composed of a separation membrane with a specific pore size, and the small holes evenly distributed on the outer wall of the chromatography column (16) allow the liquid permeating the membrane to pass through smoothly.

7. The molecular sieving collagen desalting and concentration equipment according to claim 5, characterized in that, Liquid accumulation spaces are provided between the inner wall of the screening tank (4), the outer wall of the chromatography column (16), and the bottom outer wall of the chromatography column (16).

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