Low-temperature operating device

Abstract

The utility model discloses a low-temperature operating device, which relates to the technical field of biological experimental instruments and comprises a low-temperature operating box, a partition plate is arranged in the low-temperature operating box, an inner cavity of the low-temperature operating box comprises an accommodating cavity and a reaction cavity by the partition plate, the accommodating cavity is filled with refrigerating fluid, and a reaction container is arranged in the reaction cavity; the cover plate is clamped with the port of the low-temperature operation box, and a plurality of clamping holes are uniformly distributed in the cover plate; the low-temperature operation device solves the problems that when an experiment is carried out in a low-temperature environment, due to the fact that the operation process is long, the ice plate used for maintaining the reaction temperature is gradually melted, and the experiment reaction temperatures carried out in different periods are different, the experiment time is shortened, and the experiment time is shortened. And the technical problem that the experimental result is deviated or the same process effect cannot be re-etched is solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological experiment instrument technical field, especially a low temperature operation device. BACKGROUND

[0002] At present, in the laboratory operation process in the fields such as biology, medicine, especially when carrying out some research and development verification experiments, multiple small volume samples are often operated simultaneously, especially protein crosslinking process, often requires operating under low temperature environment, to meet the experimental demand, most selected protein crosslinking operation on ice plate, but ice plate gradually melts along with the operation, leading to uncontrollable reaction temperature, and operating in an open space, the time consumed in the process of multiple experiments is too long, leading to different reaction temperature of protein crosslinking operation in different periods, chemical reaction temperature cannot be kept consistent, finally the whole experiment can appear the result of wrong result direction or cannot be reproduced the same process effect.

[0003] In summary, developing a low temperature operation device for providing stable low temperature environment for low temperature experiment operation is a problem to be solved by the person skilled in the art. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a low temperature operation device, solve the technical problem that present scheme cannot provide stable reaction temperature for low temperature operation.

[0005] To achieve the above-mentioned purpose, the utility model provides a low temperature operation device, including:

[0006] Low temperature operation box, inside being equipped with the baffle, the baffle makes low temperature operation box inner chamber including containing chamber and reaction chamber, containing chamber is filled with frozen liquid, and reaction chamber is provided with reaction container;

[0007] Cover plate, cover plate and second operation box port joint, cover plate is evenly equipped with a plurality of joint holes;

[0008] Heat preservation film, cover set in the end face of cover plate, is used for plugging each joint hole.

[0009] Preferably, the baffle is parallel to the outer wall of the low temperature operation box, and a heat preservation ring is arranged between the baffle and the inner wall of the low temperature operation box, the heat preservation ring seals the containing chamber.

[0010] Preferably, the edge of the cover plate is provided with a plurality of clamping members, the clamping members are clamped with buckles on the side wall of the low temperature operation box, and each even joint hole on the cover plate is communicated with the reaction chamber.

[0011] Preferably, the distance between the bottom surface of the reaction chamber and the cover plate is less than the height of the reaction container, so that the port of the reaction container is located outside the low temperature operation box, and the reaction container can be marked.

[0012] Preferably, the temperature sensor is arranged on the side of the cover plate close to the reaction cavity, and is used for detecting the temperature in the low-temperature operation box.

[0013] Preferably, the heat preservation film is a disposable plastic film, and the heat preservation film is arranged on the end face of the cover plate, and when the reaction container is arranged in the clamping hole, the reaction container penetrates the heat preservation film, and part of the heat preservation film is clamped between the side wall of the reaction container and the clamping hole.

[0014] Preferably, the low-temperature operation box is made of polypropylene material, the partition plate is made of stainless steel material, and the cover plate is made of transparent acrylic plate.

[0015] Preferably, the connecting piece is arranged in the side wall of the low-temperature operation box and sequentially penetrates the low-temperature operation box, the heat preservation ring and the partition plate.

[0016] With respect to the above background technology, the low-temperature operation device provided by the utility model comprises: a low-temperature operation box, a partition plate is arranged in the inner cavity of the low-temperature operation box, the partition plate divides the inner cavity of the low-temperature operation box into a containing cavity and a reaction cavity, wherein the containing cavity is arranged around the outer periphery of the reaction cavity, the containing cavity is filled with frozen liquid, the frozen liquid absorbs heat from the reaction cavity through the partition plate, so that the temperature in the reaction cavity reaches the target range of low-temperature reaction, in addition, a cover plate is clamped at the port of the low-temperature operation box, the cover plate separates the inner cavity of the low-temperature operation box from the outside, reduces the air convection between the inner cavity of the low-temperature operation box and the outside, slows down the temperature rising speed in the low-temperature operation box, further, a plurality of clamping holes are uniformly arranged on the cover plate, the clamping hole has a hole diameter matched with the side wall of the reaction container, the reaction container is inserted into the reaction cavity through the clamping hole, so that the reactants in the reaction container are in a preset low-temperature environment, and the reaction can be ensured to be normally carried out, a heat preservation film is arranged on the end face of the cover plate, the heat preservation film is used for plugging the clamping hole without the reaction container, the heat preservation property of the low-temperature operation box is further improved, and the temperature environment of each reaction container during the reaction is ensured to be the same. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the drawings needed to be used in the following embodiment or prior art description will be briefly introduced, and obviously, the drawings in the following description are only the embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative labor.

[0018] Figure 1 The explosion diagram of the low-temperature operation device provided by the embodiments of the utility model.

[0019] 1-low temperature operation box; 2- partition; 3- containing cavity; 4- reaction cavity; 5- cover plate; 51- clamping hole; 6- heat preservation film; 7- clamping piece; 8- buckle; 9- temperature sensor; 10- temperature display; 11- connecting piece. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0021] In order for those skilled in the art to better understand the technical solutions of the present application, the present application will be further described in detail below with reference to the drawings and specific embodiments.

[0022] The low temperature operation device comprises a low temperature operation box 1, a partition 2 is arranged in the inner cavity of the low temperature operation box 1, the low temperature operation box 1 is divided into two parts by the partition 2, that is, a reaction cavity 4 located at the center of the low temperature operation box 1 and a containing cavity 3 arranged at the outer periphery of the reaction cavity 4, wherein the reaction cavity 4 is used for placing a reaction container to be reacted, and a refrigerant is poured into the containing cavity 3, the refrigerant absorbs the temperature in the reaction cavity 4 through the partition 2, and provides a suitable low temperature environment for the reaction container, a cover plate 5 is clamped at the port of the low temperature operation box 1, the cover plate 5 isolates the low temperature operation box 1 from the outside, avoids air convection between the inside of the low temperature operation box 1 and the outside, and reduces the heat preservation effect of the low temperature operation box 1, further, a plurality of clamping holes 51 are arranged on the cover plate 5, the clamping holes 51 are uniformly arranged, the clamping holes 51 can be attached to the side wall of the reaction container, the reaction container is inserted into the inside of the low temperature operation box 1 through the clamping holes 51, so that the reaction container is in a suitable low temperature environment, and the reaction is ensured to be normal, in addition, a heat preservation film 6 is arranged on the end face of the cover plate 5, the heat preservation film 6 is pre-covered on the cover plate 5, the clamping holes 51 are blocked, and the cold air in the low temperature operation box 1 is prevented from escaping, when the reaction container needs to be arranged in the clamping hole 51, the end part of the reaction container penetrates the heat preservation film 6, the heat preservation film 6 continues to block the unused clamping holes 51, so that when a plurality of reaction containers are processed, the reaction temperatures of the reaction containers are all in the appropriate range, and the experimental results obtained through the reaction are more accurate.

[0023] Please refer to the drawings in the description Figure 1The partition 2 is parallel to the side wall of the cryogenic operation box 1 and is located at the center of the cryogenic operation box 1, dividing the inner cavity of the cryogenic operation box 1 into a non-connected receiving cavity 3 and a reaction cavity 4. That is, the area enclosed by the partition 2 is the reaction cavity 4, and the interlayer between the partition 2 and the side wall of the cryogenic operation box 1 is the receiving cavity 3. The reaction cavity 4 is filled with a refrigerant, which can evenly absorb the heat in the reaction cavity 4 and lower the temperature in the reaction cavity 4 to a suitable temperature. An insulation ring (not shown in the figure) is also provided between the partition 2 and the cryogenic operation box 1. The insulation ring is located on the top of the receiving cavity 3 and fits against the end face of the cover plate 5. The insulation ring further enhances the sealing of the receiving cavity 3. Preferably, the insulation ring can be made of elastic rubber. When the refrigerant acts on the insulation ring, the insulation ring undergoes a certain degree of deformation, which relieves the stress caused by temperature changes and prevents the insulation ring from being damaged due to temperature changes, thus reducing the insulation performance of the cryogenic operation device.

[0024] Please continue to refer to the instruction manual appendix. Figure 1 Several snap-fit ​​pieces 7 are provided at the edge of the cover plate 5, and buckles 8 are provided at the edge of the end of the cryogenic operation box 1. After the cover plate 5 is placed over the port of the cryogenic operation box 1, the snap-fit ​​pieces 7 and buckles 8 are aligned, and the edge of the cover plate 5 is pressed to snap the snap-fit ​​pieces 7 and buckles 8 together, thus completing the fixed installation of the cryogenic operation box 1 and the cover plate 5. It should be noted that the snap-fit ​​holes 51 on the cover plate 5 are all located within the area enclosed by the partition plate 2, ensuring that the reaction containers fixed through the snap-fit ​​holes 51 are all inserted into the reaction chamber 4. The partition plate 2 separates the inner cavity of the cryogenic operation box 1, so that the reaction containers do not come into direct contact with the cryogenic liquid, avoiding the need to clean the outer wall of the reaction containers after the reaction is completed, and simplifying the operation steps. In addition, the distance between the bottom surface of the inner cavity of the cryogenic operation box 1 and the cover plate 5 is less than the height of the reaction containers. That is, when the reaction containers are completely inserted into the reaction chamber 4, the port of the reaction containers is still outside the cryogenic operation box 1, and can be marked on the outer end of the reaction containers.

[0025] Preferably, the cryogenic operation box 1 can be made of polypropylene material, so that the cryogenic operation box 1 has a certain heat preservation performance and high cold resistance, avoiding damage to the cryogenic operation box 1 due to contact with the coolant. The partition 2 is used to separate the reaction chamber 4 and the containment chamber 3, and the coolant in the containment chamber 3 absorbs the heat in the reaction chamber 4 through the partition 2. The partition 2 is required to have a certain strength and good thermal conductivity. In some embodiments of this application, the partition 2 is made of stainless steel material. The coolant absorbs heat from the containment chamber 3 efficiently through the stainless steel plate, so that the temperature in the containment chamber 3 can quickly reach the suitable reaction temperature. Furthermore, the cover plate 5 can be made of transparent acrylic plate, so that the operator can observe the reaction in the reaction vessel in real time.

[0026] Preferably, the distance between the side wall of the low-temperature operation box 1 and the partition plate 2 is 2-3 cm, and the distance between the bottom surface of the low-temperature operation box 1 and the cover plate 5 is half to two-thirds of the length of the reaction container, so that the reaction container has sufficient length for marking while ensuring that the reactants in the reaction container are all in the reaction cavity 4.

[0027] A connecting piece 11 is provided on the side wall of the low-temperature operation box 1, which penetrates the side wall of the low-temperature operation box 1, the heat preservation ring, and the partition plate 2 in sequence. The connecting piece 11 fixes and connects the above-mentioned components, so that the heat preservation ring is stably clamped between the low-temperature operation box 1 and the partition plate 2, avoiding the heat preservation ring from falling off due to size reduction caused by low temperature, which affects the experimental process. Please refer to the drawings. Figure 1 A temperature sensor 9 is provided on the side end surface of the cover plate 5 close to the reaction cavity 4. When the cover plate 5 is fixedly connected with the low-temperature operation box 1, the temperature sensor 9 is located within the range surrounded by the partition plate 2, that is, the temperature sensor 9 is located in the reaction cavity 4. In addition, the temperature sensor 9 is connected with a temperature display 10 through a cable signal. The temperature display 10 is located outside the low-temperature operation box 1. The temperature display 10 receives the electrical signal detected by the temperature sensor 9 and displays the temperature in the reaction cavity 4 on the display screen, so that the operator can intuitively understand whether the temperature of the reaction container meets the standard.

[0028] Preferably, the heat preservation film 6 can be a disposable plastic film, which can be replaceably laid on the end surface of the cover plate 5. After the low-temperature operation box 1 and the cover plate 5 are assembled, a layer of heat preservation film 6 is arranged on the partition plate 2, which seals the clamping holes 51. When low-temperature operation is performed, the reaction container penetrates the heat preservation film 6 through external force. In the process of penetrating the heat preservation film 6, the heat preservation film 6 deforms and passes through the clamping hole 51 together with the reaction container. When the deformation exceeds the bearing range of the heat preservation film 6, the reaction container penetrates the heat preservation film 6 and clamps part of the heat preservation film 6 between the clamping hole 51 and the side wall of the reaction container. The heat preservation film 6 fills the gap between the clamping hole 51 and the reaction container, further stabilizes the setting state of the reaction container, and also enhances the airtightness of the low-temperature operation device, avoiding the escape of cold air in the reaction cavity 4 to the outside.

[0029] In one embodiment of the utility model, before low temperature operation, fill in the accommodating cavity 3 of low temperature operation box 1 with appropriate amount of frozen liquid, put low temperature operation box 1 into the freezer and carry out cooling, cover heat preservation film 6 on the end face of cover plate 5 during the freezing process of low temperature operation box 1, then take out low temperature operation box 1 after completing freezing, the clamping piece 7 of cover plate 5 is clamped with the buckle 8 of low temperature operation box 1, complete the assembly of cover plate 5 and low temperature operation box 1, detect temperature through temperature sensor 9, when the temperature in low temperature operation box 1 satisfies the experimental temperature, insert the reaction container into the clamping hole 51, mark the part of reaction container outside the reaction cavity 4, facilitate the record of each group experimental result.

[0030] It should be noted that the relative terms, such as first and second, in the description are used only to differentiate one entity from another entity, and do not necessarily require or imply any such actual relationship or order between or among these entities.

[0031] The principles and implementation modes of the utility model are described by applying specific examples in the present document, and the above description of the examples is only for helping to understand the method of the utility model and its core idea. It should be pointed out that, for ordinary skilled persons in the technical field, without departing from the principles of the utility model, the utility model can be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the utility model.

Claims

1. A cryogenic operation device, characterized in that, include: The low-temperature operation box (1) has a partition (2) inside, which divides the inner cavity of the low-temperature operation box (1) into a receiving cavity (3) and a reaction cavity (4). The receiving cavity (3) is filled with a cryogenic liquid, and the reaction cavity (4) is equipped with a reaction container. Cover plate (5), the cover plate (5) is snapped into the port of the low temperature operation box (1), and a plurality of snap-fit ​​holes (51) are evenly distributed on the cover plate (5). A heat-insulating film (6) is applied to the end face of the cover plate (5) to seal each of the snap-fit ​​holes (51).

2. The cryogenic operation device according to claim 1, characterized in that, The partition (2) is parallel to the outer wall of the low-temperature operation box (1), and a heat-insulating ring is provided between the partition (2) and the inner wall of the low-temperature operation box (1) to seal the receiving cavity (3).

3. The cryogenic operation device according to claim 2, characterized in that, The cover plate (5) has several snap-fit ​​parts (7) along its edge. The snap-fit ​​parts (7) are snapped into the buckles (8) on the side wall of the low temperature operation box (1). Each snap-fit ​​hole (51) on the cover plate (5) is connected to the reaction chamber (4).

4. The cryogenic operation device according to claim 3, characterized in that, The distance between the bottom surface of the reaction chamber (4) and the cover plate (5) is less than the height of the reaction container, so that the port of the reaction container is located outside the low temperature operation box (1), and the reaction container can be marked.

5. The cryogenic operation device according to claim 4, characterized in that, A temperature sensor (9) is provided on the side of the cover plate (5) near the reaction chamber (4). The temperature sensor (9) is used to detect the temperature inside the low-temperature operation box (1). The temperature sensor (9) is connected to a temperature display (10), which displays the temperature detected by the temperature sensor (9).

6. The cryogenic operation apparatus according to claim 1, characterized in that, The heat insulation film (6) is specifically a disposable plastic film. The heat insulation film (6) is replaceably provided on the end face of the cover plate (5). When the reaction container is placed in the snap-fit ​​hole (51), the reaction container penetrates the heat insulation film (6), and part of the heat insulation film (6) is sandwiched between the side wall of the reaction container and the snap-fit ​​hole (51).

7. The cryogenic operation apparatus according to claim 1, characterized in that, The cryogenic operation box (1) is made of polypropylene, the partition (2) is made of stainless steel, and the cover (5) is made of transparent acrylic sheet.

8. The cryogenic operation apparatus according to claim 2, characterized in that, A connector (11) is provided on the side wall of the low-temperature operation box (1), and the connector (11) passes through the low-temperature operation box (1), the insulation ring and the partition (2) in sequence.

Images