Protein purification device

Abstract

The utility model relates to the technical field of protein purification devices, and solves the problems that in the process of using a protein purification instrument, a pipeline needs to continuously extract a solution in a bottle body, a user only needs to simply place the bottle body on the top surface of the protein purification instrument, a structure for fixing the bottle body is lacked, the bottle body is easily influenced by external force and topples over, and the use cost is high. In addition, the problems that the solution intrudes into the protein purification instrument, and the protein purification instrument is damaged are also solved. The protein purification device comprises a protein purification instrument, the top surface of the protein purification instrument is fixedly connected with a plurality of positioning assemblies, and the side surface of the protein purification instrument is fixedly connected with a wire clamp assembly; the positioning assembly comprises stretching damping rods which are fixedly connected to the top surface of the protein purification instrument in a rectangular array manner, the top ends of the stretching damping rods are fixedly connected with positioning blocks, grooves are formed in the side surfaces of the positioning blocks in a rectangular array manner, and rotating blocks are rotationally connected to the interiors of the grooves.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein purification device technical field, specifically is a kind of protein purification device. BACKGROUND

[0002] The gene recombination albumin high-efficiency purification device with publication No. CN210855920U includes a mounting bracket, a machine case is arranged on one side of the top of the mounting bracket, a processing box is arranged on one side of the top end of the mounting bracket, a liquid supply pump is fixedly installed at the bottom end of the mounting bracket, a liquid storage cavity is arranged in the machine case, and a liquid outlet pipeline is arranged at the bottom end of the liquid storage cavity. The beneficial effect is that the processing box is arranged above the top end of the gravity column, a stirrer and an air outlet pipeline are arranged in the processing box, the stirrer is used to stir the protein raw pulp, and the air outlet pipeline blows air bubbles into the raw pulp through an air outlet hole. According to the biological characteristics of proteins, appropriate stirring and vibration can release proteins from the raw pulp or dissolved state while maintaining the biological activity of proteins.

[0003] The processing box is equivalent to a pretreatment device for protein purification, which can accelerate the precipitation of albumin in the gravity column and improve the purification efficiency.

[0004] The technical solution in the comparative document has the effect of improving the purification efficiency, but the defects are also more obvious. For example, during the use of the protein purification instrument, the pipeline needs to continuously extract the solution in the bottle body, and the user usually only places the bottle body simply on the top surface of the protein purification instrument, lacks a structure to fix it, is easily affected by external force to appear to dump, causes the waste of solution, and the solution invades the inside of the protein purification instrument, which also causes the damage of the equipment. UTILITY MODEL CONTENT

[0005] To overcome the shortcomings of the prior art, the utility model provides a protein purification device, which solves the problem that during the use of the protein purification instrument, the pipeline needs to continuously extract the solution in the bottle body, and the user usually only places the bottle body simply on the top surface of the protein purification instrument, lacks a structure to fix it, is easily affected by external force to appear to dump, causes the waste of solution, and the solution invades the inside of the protein purification instrument, which also causes the damage of the equipment.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a protein purification device, comprising a protein purification instrument, a plurality of positioning assemblies are fixedly connected to the top surface of the protein purification instrument, and a wire clamp assembly is fixedly connected to the side surface of the protein purification instrument.

[0007] The positioning assembly comprises stretch damping rods fixedly connected to the top surface of the protein purifier in a rectangular array, and a positioning block is fixedly connected to the top end of the stretch damping rods, the side surface of the positioning block is provided with recesses in a rectangular array, and a rotating block is rotatably connected inside each recess, one end of the rotating block is fixedly connected to the top surface of the protein purifier through a support, and the other end of the rotating block is threadedly connected with a pressing block.

[0008] The wire clamp assembly comprises a frame, and elastic clamping plates are fixedly connected inside the frame in an axial symmetry, and a roller is rotatably connected to one end of the elastic clamping plate.

[0009] In a specific embodiment, the stretch damping rods are distributed in a rectangular array along the bottom surface of the positioning block, and the stretching direction of each stretch damping rod is perpendicular to the top surface of the positioning block.

[0010] In a specific embodiment, the rotating block is rotatably connected to the top end of the support through a rotating shaft, and the rotating plane of the rotating block is consistent with the extension direction of the recess of the positioning block.

[0011] In a specific embodiment, the pressing block is screwed into the end of the rotating block, and the clamping surface of the pressing block is provided with anti-skid lines.

[0012] In a specific embodiment, the elastic clamping plates are symmetrically arranged inside the frame, and an arc-shaped guide groove is arranged in the middle of the elastic clamping plate, and the roller is embedded in the guide groove.

[0013] In a specific embodiment, an annular groove is arranged on the inner circumferential surface of the elastic clamping plate, and the groove width is matched with the diameter of the pipeline.

[0014] Compared with the prior art, the protein purification device has the following beneficial effects:

[0015] In the technical scheme, the pressing trigger mechanism of the positioning assembly and the elastic fixation of the wire clamp assembly are cooperatively used to ensure the double stability of the bottle body and the pipeline, and prevent solution leakage or equipment damage.

[0016] The positioning assembly is provided with the stretching damping rods arranged in a rectangular array and connected with the positioning blocks, the stretching damping rods provide uniform buffer resistance when the bottle is pressed, the positioning blocks are ensured to be vertically and stably lowered, and the bottle is prevented from being inclined due to uneven stress; when the bottle is placed on the top surface of the positioning block and is pressed, the positioning block moves downwards, the side surface groove of the positioning block pushes the rotating block to rotate around the rotating shaft of the support, the extrusion block connected with the screw is driven to move towards the center synchronously, multi-directional synchronous clamping is realized, the screw connection design of the extrusion block allows the clamping force to be adjusted, the bottle body of different diameters is adapted, the anti-skid lines of the clamping surface further prevent the bottle from sliding, the problems that the bottle body is not stably fixed and the pipeline is easily detached are avoided, the positioning assembly has the characteristics of convenient operation, compact structure and strong adaptability, and the safety of the protein purification process is significantly improved. BRIEF DESCRIPTION OF DRAWINGS

[0017] The drawings described herein are used to provide further understanding of the present application, constitute a part of the present application, the illustrative embodiments of the present application and the description thereof are used to explain the present application, and do not constitute improper limitation on the present application. In the drawings:

[0018] Figure 1 It is a whole structure schematic view of the utility model;

[0019] Figure 2 It is a positioning assembly structure schematic view of the utility model;

[0020] Figure 3 It is a wire clamp assembly structure schematic view of the utility model;

[0021] Figure 4 It is a split structure schematic view of the utility model.

[0022] In the drawings: 1, protein purification instrument; 2, positioning assembly; 21, stretching damping rod; 22, positioning block; 23, rotating block; 24, support; 25, extrusion block; 3, wire clamp assembly; 31, frame; 32, elastic clamping plate; 33, roller. DETAILED DESCRIPTION

[0023] The embodiments of the present application will be described in detail below with the drawings and examples, so that the realization process of how to apply technical means to solve technical problems and achieve technical effects of the present application can be fully understood and implemented.

[0024] Figures 1-4 For an embodiment of the utility model, a protein purification device, comprising a protein purification instrument 1, characterized by: the top surface of the protein purification instrument 1 is fixedly connected with a plurality of positioning assemblies 2, and the side surface of the protein purification instrument 1 is fixedly connected with a wire clamp assembly 3.

[0025] The specific problem that the specific embodiment aims at is that in the process of using the protein purification instrument, the pipeline needs to continuously extract the solution in the bottle, and the user generally only simply places the bottle on the top surface of the protein purification instrument, lacks a structure for fixing it, is easy to be affected by external force and appear to be tilted, causes the waste of the solution, and the solution invades the inside of the protein purification instrument, which also causes the damage of the equipment. The utility model utilizes the synergistic effect of the pressing trigger mechanism of the positioning assembly 2 and the elastic fixing of the wire clamp assembly 3, ensures that the bottle and the pipeline are double-stable, and prevents the solution from leaking or the equipment from being damaged.

[0026] The positioning assembly 2 includes a stretch damping rod 21 fixedly connected to the top surface of the protein purification instrument 1 in a rectangular array, the top end of the stretch damping rod 21 is fixedly connected with a positioning block 22, the side surface of the positioning block 22 is provided with a recess in a rectangular array, the inside of the recess is rotatably connected with a rotating block 23, one end of the rotating block 23 is fixedly connected with the top surface of the protein purification instrument 1 through a support 24, the other end of the rotating block 23 is screwedly connected with a pressing block 25, in the specific embodiment, the stretch damping rods 21 are distributed in a rectangular array along the bottom surface of the positioning block 22, and the extension direction of each stretch damping rod 21 is perpendicular to the top surface of the positioning block 22, the rotating block 23 is rotatably connected with the top end of the support 24 through a rotating shaft, and the rotating plane of the rotating block 23 is consistent with the extension direction of the recess of the positioning block 22, the pressing block 25 is screwed into the end portion of the rotating block 23, and the clamping surface of the pressing block 25 is provided with anti-skid lines. The stretch damping rods 21 in a rectangular array are connected with the positioning block 22, the stretch damping rods 21 provide uniform buffer resistance when the bottle is pressed down, ensure that the positioning block 22 stably descends vertically, and avoid that the bottle is inclined due to uneven stress; when the bottle is placed on the top surface of the positioning block 22 and is pressed down, the positioning block 22 moves downward, the side surface recess of the positioning block 22 drives the rotating block 23 to rotate around the rotating shaft of the support 24, drives the screwedly connected pressing block 25 to move synchronously to the center, and realizes multi-directional synchronous clamping; the screwedly connected design of the pressing block 25 allows the clamping force to be adjusted, and is suitable for bottles of different diameters; the anti-skid lines on the clamping surface further prevent the bottle from sliding, thereby avoiding the problems that the bottle is not fixed stably and the pipeline is easy to fall off, and the utility model has the characteristics of convenient operation, compact structure and strong adaptability, and significantly improves the safety of the protein purification process.

[0027] In the specific embodiment, the wire clamp assembly 3 includes a frame 31, the inside of the frame 31 is fixedly connected with an elastic clamping plate 32 in an axial symmetry, one end of the elastic clamping plate 32 is rotatably connected with a roller 33; the elastic clamping plate 32 is symmetrically arranged on the inside of the frame 31, and the middle part of the elastic clamping plate 32 is provided with an arc-shaped guide groove, and the roller 33 is embedded in the guide groove; the inner circumferential surface of the clamping plate 32 is provided with an annular groove, and the width of the groove is suitable for the diameter of the pipeline.

[0028] The elastic clamping plate 32 is arranged in the frame 31 in an axisymmetric manner, and is self-adapted to clamp pipes with different diameters through its elasticity, and the rotation of the roller 33 reduces the friction resistance when the pipe is pressed into the wire clamp assembly 3; the rotation of the roller 33 reduces the abrasion of the cable, and the open structure of the frame 31 facilitates the quick insertion and extraction of the pipe.

[0029] The control mode of the utility model is automatically controlled through the controller, the control circuit of the controller can be realized through simple programming of the person skilled in the art, the power supply also belongs to the common knowledge in the field, and the utility model is mainly used for protecting the mechanical device, so the control mode and the circuit connection of the utility model will not be explained in detail.

[0030] It should be noted that in this document, the terms "comprise", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, so that a process, method, article, or apparatus that comprises a list of elements does not only include those elements, but also includes other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0031] Although the embodiments of the utility model have been shown and described, it can be understood by those of ordinary skill in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A protein purification apparatus comprising a protein purification instrument (1), characterized in that: The top surface of the protein purification instrument (1) is fixedly connected with a plurality of positioning assemblies (2), and the side surface of the protein purification instrument (1) is fixedly connected with a wire clamp assembly (3); The positioning assembly (2) comprises a tensile damping rod (21) fixedly connected to the top surface of the protein purification instrument (1) in a rectangular array, the top end of the tensile damping rod (21) is fixedly connected with a positioning block (22), the side surface of the positioning block (22) is provided with a recess in a rectangular array, and the recess is rotatably connected with a rotating block (23); one end of the rotating block (23) is fixedly connected with the top surface of the protein purification instrument (1) through a support (24), and the other end of the rotating block (23) is threadedly connected with a pressing block (25). The wire clamp assembly (3) comprises a frame (31), and the frame (31) is fixedly connected with an elastic clamping plate (32) in an axisymmetric manner inside the frame (31), and one end of the elastic clamping plate (32) is rotatably connected with a roller (33).

2. A protein purification device according to claim 1, characterized in that: The tensile damping rods (21) are distributed in a rectangular array along the bottom surface of the positioning block (22), and the stretching direction of each tensile damping rod (21) is perpendicular to the top surface of the positioning block (22).

3. A protein purification device according to claim 1, wherein: The rotating block (23) is rotatably connected with the top end of the support (24) through a rotating shaft, and the rotating plane of the rotating block (23) is consistent with the extension direction of the recess of the positioning block (22).

4. A protein purification device according to claim 1, characterized in that: The pressing block (25) is screwed into the end of the rotating block (23), and the clamping surface of the pressing block (25) is provided with anti-skid lines.

5. A protein purification device according to claim 1, wherein: The elastic clamping plate (32) is symmetrically arranged inside the frame (31), and an arc-shaped guide groove is arranged in the middle of the elastic clamping plate (32), and the roller (33) is embedded in the guide groove.

6. A protein purification device according to claim 1, wherein: The inner circumferential surface of the elastic clamping plate (32) is provided with an annular recess, and the recess width is matched with the pipe diameter. The inner circumferential surface of the elastic clamping plate (32) is provided with an annular recess, and the recess width is matched with the pipe diameter.

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