Fermentation device

Abstract

The utility model discloses a fermentation device which comprises a fermentation tank and a sterile water tank, the fermentation tank is communicated with an air pipeline, and the fermentation tank is provided with a stirring mechanism; the sterile water tank is communicated with the air pipeline through a first pipeline, and a spray head is arranged on the first pipeline; or the air pipeline is communicated with the sterile water tank through a second pipeline, and the sterile water tank is communicated with the air pipeline through a third pipeline. According to the fermentation device disclosed by the utility model, sterile dry and hot air is firstly introduced into the sterile water tank for washing before entering the fermentation tank, or is sprayed into the air pipeline through the sterile water tank to humidify and cool the sterile dry and hot air, so that the concentrations of dissolved oxygen and negative oxygen ions in the sterile dry and hot air are increased; the content of dissolved oxygen in fermentation liquor in the fermentation tank can be increased, fermentation of microorganisms is facilitated, and the fermentation efficiency can be improved. The water is atomized into tiny liquid drops through the arrangement of the spraying head, the contact area between the water and the air can be increased, mass transfer between the water and the air is accelerated, and the water is evenly distributed in the sterile dry and hot air.

Description

TECHNICAL FIELD

[0001] The utility model relates to fermentation technical field more particularly, relate to a kind of fermentation device. BACKGROUND

[0002] The sterile air for the existing conventional liquid submerged fermentation is the gas generated by air compressor with temperature about 100 ℃, which is first cooled to 15 ℃-30 ℃ by a cooler to remove the water vapor in the cooled air, then warmed to 40 ℃-70 ℃, and then enters a 2-stage-3-stage membrane filter to produce sterile dry hot air. The sterile dry hot air generated is directly fed into the fermentation tank, and the increased dissolved oxygen under the action of high-strength stirrer facilitates microbial fermentation, but the utilization rate of oxygen in the air is relatively low, generally only about 3%.

[0003] The disadvantage of the prior art is that the dissolved oxygen of dry hot air in the fermentation liquid is relatively small, therefore, how to improve the dissolved oxygen of dry hot air in the fermentation liquid to improve the fermentation efficiency becomes a technical problem to be solved by the technical personnel in the field. SUMMARY

[0004] Therefore, the purpose of the utility model is to provide a fermentation device to improve the dissolved oxygen of dry hot air in the fermentation liquid and improve the fermentation efficiency.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0006] A fermentation device comprises:

[0007] A fermentation tank, which is communicated with an air pipeline, is provided with a stirring mechanism.

[0008] A sterile water tank is communicated with the air pipeline through a first pipeline, and a spray head is arranged on the first pipeline; or the air pipeline is communicated with the sterile water tank through a second pipeline, and the sterile water tank is communicated with the air pipeline through a third pipeline.

[0009] Optionally, in the fermentation device, the fermentation tank comprises a first fermentation tank or a second fermentation tank, the air pipeline comprises a first air pipeline or a second air pipeline, the sterile water tank comprises a first sterile water tank or a second sterile water tank, the first fermentation tank is communicated with the first air pipeline, the first sterile water tank is communicated with the first air pipeline through the first pipeline, and the spray head is arranged on the first pipeline; or,

[0010] The second fermentation tank is communicated with the second air pipeline, the second air pipeline is communicated with the second sterile water tank through the second pipeline, and the second sterile water tank is communicated with the second air pipeline through the third pipeline.

[0011] Optionally, in the fermentation device, a water pump is arranged on the first pipeline, the water pump is arranged downstream of the first sterile water tank, the first pipeline is provided with a bypass pipe connected in parallel with the water pump, and a bypass valve is arranged on the bypass pipe.

[0012] Optionally, in the fermentation device, an ammonia water pipeline is further arranged, the ammonia water pipeline comprises an ammonia water main pipe and first and second ammonia water branch pipes connected with the ammonia water main pipe respectively, the first ammonia water branch pipe is connected with the first pipeline, and the second ammonia water branch pipe is connected with the first air pipeline.

[0013] Optionally, in the fermentation device, a first valve is arranged on the ammonia water main pipe, a second valve is arranged on the first ammonia water branch pipe, and a third valve is arranged on the second ammonia water branch pipe.

[0014] Optionally, in the fermentation device, a filter is arranged on the first pipeline, and the filter is arranged between the first sterile water tank and the water pump.

[0015] Optionally, in the fermentation device, the first air pipeline is connected with a first steam pipeline, and a fourth valve is arranged on the first steam pipeline.

[0016] The first pipeline is connected with a second steam pipeline, the second steam pipeline is arranged upstream of the filter, and a fifth valve is arranged on the second steam pipeline.

[0017] Optionally, in the fermentation device, a first pressure gauge is arranged on the first sterile water tank; and / or,

[0018] a second pressure gauge is arranged on the first pipeline; and / or,

[0019] a third pressure gauge is arranged on the first fermentation tank.

[0020] Optionally, in the fermentation device, a sixth valve and a seventh valve are arranged on the first air pipeline, the second ammonia water branch pipe is arranged downstream of the sixth valve, and the seventh valve is arranged downstream of the second steam pipeline.

[0021] Optionally, in the fermentation device, an eighth valve, a ninth valve and a tenth valve are arranged on the first pipeline, the eighth valve is arranged between the first sterile water tank and the first steam pipeline, the ninth valve is arranged between the water pump and the spray head, the tenth valve is arranged between the filter and the water pump, and the first ammonia water branch pipe is arranged downstream of the tenth valve.

[0022] From the above scheme can be seen, the utility model discloses a fermentation device, make sterile dry hot air first into the sterile water tank and carry out water washing before entering the fermentation tank, or through the sterile water tank to the air pipeline inside spray, the concentration of dissolved oxygen and negative oxygen ion in sterile dry hot air increase, can improve the content of dissolved oxygen in the fermentation broth in the fermentation tank, be favorable to the fermentation of microorganism, can improve the fermentation efficiency. The setting of the spray head atomizes water into tiny droplets, can increase the contact area of water and air, accelerate the mass transfer with air, make water evenly distribute in sterile dry hot air, can uniformly cool sterile dry hot air. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or prior art, the following will briefly introduce the drawing needed to be used in embodiment or prior art description, obviously, the drawing in the following description only some embodiments of the utility model, for ordinary skilled person in the art, under the premise of not paying the creative labor, still can obtain other drawings according to these drawings.

[0024] Figure 1 For the schematic diagram of the fermentation device disclosed in the embodiments of the utility model Figure One ;

[0025] Figure 2 For the schematic diagram of the fermentation device disclosed in the embodiments of the utility model Figure Two .

[0026] Among them, A is sterile water tank, A1 is first sterile water tank, A2 is second sterile water tank, B is filter, C is water pump, D is spray head, E is fermentation tank, E1 is first fermentation tank, E2 is second fermentation tank, E3 is stirring mechanism, I is heater, J is primary filter, K is fine filter;

[0027] F is first pressure gauge, G is second pressure gauge, H is third pressure gauge;

[0028] 100 is air pipe, 100-1 is first air pipe, 100-2 is second air pipe, 110 is sixth valve, 120 is seventh valve, 130 is twelfth valve, 140 is fifteenth valve, 150 is sixteenth valve, 160 is check valve;

[0029] 200 is second pipe, 210 is second pipe valve;

[0030] 300 is third pipe, 310 is third pipe valve;

[0031] 400 is a pipe, 410 is eighth valve, 420 is ninth valve, 430 is tenth valve;

[0032] 500 is a bypass pipe, and 510 is a bypass valve;

[0033] 600 is an ammonia water pipe, 610 is an ammonia water main pipe, 611 is a first valve, 620 is a first ammonia water branch pipe, 621 is a second valve, 630 is a second ammonia water branch pipe, and 631 is a third valve;

[0034] 700 is a second steam pipe, 710 is a fifth valve, 720 is a third steam pipe, 721 is a seventeenth valve, 730 is a fourth steam pipe, and 731 is an eighteenth valve;

[0035] 800 is a first steam pipe, and 810 is a fourth valve;

[0036] 900 is a fourth pipe, 910 is an eleventh valve,

[0037] 10 is a first exhaust valve, 20 is a second exhaust valve, 30 is a third exhaust valve, 40 is a fourth exhaust valve, 50 is a fifth exhaust valve, 60 is a sixth exhaust valve, and 70 is a seventh exhaust valve. DETAILED DESCRIPTION

[0038] The core of the present application is to disclose a fermentation device to improve the dissolved oxygen of dry hot air in fermentation liquid and improve the fermentation efficiency.

[0039] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0040] As shown in Figure 1 and Figure 2 The present application discloses a fermentation device, which comprises a fermentation tank E and a sterile water tank A. The sterile dry hot air entering the fermentation tank E is cooled and humidified by the sterile water tank A to improve the dissolved oxygen of the sterile dry hot air in the fermentation liquid.

[0041] The fermentation tank E is connected to the air pipe 100, and the sterile dry hot air is introduced into the fermentation tank E through the air pipe 100 to provide dissolved oxygen for the culture medium in the fermentation tank E. The fermentation tank E comprises a stirring mechanism E3, which stirs the culture medium in the fermentation tank E, increases the contact area of the sterile dry hot air and the culture medium, and increases the dissolved oxygen. Figure 1 and Figure 2 are two different implementation modes for cooling and humidifying the sterile dry hot air, Figure 1 After the sterile dry hot air is sprayed, cooled and humidified, it is introduced into the fermentation tank E, Figure 2The sterile dry hot air is introduced into the sterile water tank A, and then introduced into the fermentation tank E after water cooling, humidification and temperature reduction. As shown in Figure 1 The sterile water tank A is connected with the air pipeline 100 through the first pipeline 400, and the first pipeline 400 is provided with a spray head D. The water in the sterile water tank A is introduced into the air pipeline 100 in the form of mist through the first pipeline 400 and the spray head D, so as to humidify and cool the sterile dry hot air. The humidification and cooling can be realized by means of pressure spraying or ultrasonic spraying.

[0042] As shown in Figure 2 The air pipeline 100 is connected with the sterile water tank A through the second pipeline 200, and the sterile water tank A is connected with the air pipeline 100 through the third pipeline 300. The sterile dry hot air is first introduced into the sterile water tank A for water cooling before being introduced into the fermentation tank E, so as to humidify and cool the sterile dry hot air. After the humidification and cooling, the sterile dry hot air is introduced into the fermentation tank E through the air pipeline 100.

[0043] The fermentation device disclosed in the embodiment of the present application can first introduce the sterile dry hot air into the sterile water tank A for water cooling before the sterile dry hot air is introduced into the fermentation tank E, or spray the sterile dry hot air into the air pipeline 100 through the sterile water tank A, so as to humidify and cool the sterile dry hot air. The concentration of dissolved oxygen and negative oxygen ions in the sterile dry hot air is increased, the content of dissolved oxygen in the fermentation liquid in the fermentation tank E is increased, the fermentation of microorganisms is facilitated, and the fermentation efficiency is improved. The water is atomized into small droplets by the spray head D, the contact area between the water and the air is increased, the mass transfer with the air is accelerated, the water is uniformly distributed in the sterile dry hot air, and the sterile dry hot air can be uniformly cooled.

[0044] It should be noted that the humidification and cooling of the sterile dry hot air need to be determined according to the fermentation requirements. The fermentation device disclosed in the present application increases the concentration of negative oxygen ions and the dissolved oxygen in the fermentation liquid by means of humidification and cooling. Any method for improving the fermentation efficiency or saving energy and reducing consumption by increasing the concentration of negative oxygen ions and the dissolved oxygen in the fermentation liquid through the humidification and cooling of the sterile dry hot air falls within the protection scope of the embodiment of the present application, such as pressure spraying or ultrasonic spraying.

[0045] Further, in order to distinguish the embodiments of Figure 1 and Figure 2 , the fermentation tank E includes a first fermentation tank E1 or a second fermentation tank E2, the air pipeline 100 includes a first air pipeline 100-1 or a second air pipeline 100-2, and the sterile water tank A includes a first sterile water tank A1 or a second sterile water tank A2. In the first embodiment, as shown in Figure 1As shown in the first embodiment, the first fermenter E1 is connected to the first air pipeline 100-1, the first sterile water tank A1 is connected to the first air pipeline 100-1 through the first pipeline 400, and the spray head D is arranged on the first pipeline 400. The water in the first sterile water tank A1 is sprayed into the first air pipeline 100-1 in the form of mist through the first pipeline 400 and the spray head D to humidify and cool the sterile dry hot air. For the first fermenter E1 with a large volume, such as the volume of the first fermenter E1 being 30 cubic meters to 300 cubic meters, this scheme is preferred to reduce the volume of the first sterile water tank A1.

[0046] In the second embodiment, as shown in the second embodiment, Figure 2 the second fermenter E2 is connected to the second air pipeline 100-2, the second air pipeline 100-2 is connected to the second sterile water tank A2 through the second pipeline 200, and the second sterile water tank A2 is connected to the second air pipeline 100-2 through the third pipeline 300, that is, the second sterile water tank A2 is connected in parallel with part of the second air pipeline 100-2. The sterile dry hot air is first introduced into the second sterile water tank A2 for water washing before entering the second fermenter E2 to humidify and cool the sterile dry hot air. After humidification and cooling, the sterile dry hot air enters the second fermenter E2 through the second air pipeline 100-2. The second sterile water tank A2 can contain cold water or ammonia water. For the second fermenter E2 with a small volume, such as the volume of the second fermenter E2 being 10 liters to 50 liters, this scheme is preferred. The selection of the scheme of the first embodiment and the scheme of the second embodiment can be determined according to the actual situation.

[0047] Hereinafter, the specific structure and composition of the fermentation device will be described by taking the scheme of the first embodiment as an example. Figure 1 As shown in the first embodiment,

[0048] Further, the spray head D and the first pipeline 400 are preferably connected through a quick chuck to form a live joint for convenient disassembly and cleaning. Of course, the spray head D and the first pipeline 400 can also be connected through a flange connection or a threaded connection. The specific type of the spray head D can be selected according to the actual situation.

[0049] Further, as shown in the first embodiment, Figure 1 the first pipeline 400 is provided with a water pump C. The water pump C provides power. The water pump C is arranged downstream of the first sterile water tank A1. The water pump C can be a frequency pump or a variable frequency pump. Preferably, the water pump C is a high-temperature pump. Specifically, the water pump C can be a high-temperature magnetic pump or other types. In order to meet the flow regulation and pressure control of the water pump C and facilitate sterilization, the first pipeline 400 is provided with a bypass pipe 500 connected in parallel with the water pump C. The bypass pipe 500 is provided with a bypass valve 510. The specific type of the bypass valve 510 can be selected according to the actual situation. Preferably, the bypass valve 510 is a diaphragm valve.

[0050] In order to further cool the sterile dry hot air, the fermentation device further comprises an ammonia water pipeline 600, as shown in Figure 1 The ammonia water pipeline 600 comprises an ammonia water main pipeline 610 and a first ammonia water branch pipeline 620 and a second ammonia water branch pipeline 630 which are in communication with the ammonia water main pipeline 610, the first ammonia water branch pipeline 620 is in communication with the first pipeline 400, and the second ammonia water branch pipeline 630 is in communication with the first air pipeline 100-1. The connection point of the first ammonia water branch pipeline 620 and the first pipeline 400 is preferably located upstream of the water pump C. Preferably, the ammonia water main pipeline 610, the first ammonia water branch pipeline 620 and the second ammonia water branch pipeline 630 are connected by a three-way connector.

[0051] The ammonia water can flow into the first pipeline 400 through the first ammonia water branch pipeline 620, and the ammonia water and the water in the first sterile water tank A1 are mixed in the first pipeline 400 and sprayed into the first air pipeline 100-1 through the spray head D to cool and humidify the sterile dry hot air. The ammonia water can also be directly supplemented into the first air pipeline 100-1 through the second ammonia water branch pipeline 630 to cool and humidify the sterile dry hot air.

[0052] Further, as shown in Figure 1 In order to control the communication and disconnection of the ammonia water pipeline 600, a first valve 611 is arranged on the ammonia water main pipeline 610, a second valve 621 is arranged on the first ammonia water branch pipeline 620, and a sixth exhaust valve 60 is arranged on the second valve 621 for sterilization and disinfection. A third valve 631 is arranged on the second ammonia water branch pipeline 630. The second valve 621 shown in the figure is provided with two, and is arranged near the first pipeline 400 and near the ammonia water main pipeline 610, respectively. The arrangement of the two second valves 621 can be used as backup for each other, and at the same time facilitates the sterilization of the ammonia water pipeline 600. The specific type of the first valve 611, the second valve 621 and the third valve 631 is not limited, and a diaphragm valve is preferably selected, and a stop valve can also be selected, which needs to meet the requirements of sterility, corrosion resistance, easy cleaning and reliable sealing.

[0053] Further, as shown in Figure 1 In order to filter the water entering the water pump C and prevent the water pump C from being blocked, a filter B is arranged on the first pipeline 400, the filter B is arranged between the first sterile water tank A1 and the water pump C, the filter B is preferably a metal filter, and the specific type of the filter B can be selected according to actual needs.

[0054] Further, in order to sterilize the first fermenter E1, the filter B and the pipes and valves through which water and sterile dry hot air pass, the first air pipe 100-1 is connected with the first steam pipe 800, the fourth valve 810 is arranged on the first steam pipe 800, the first pipe 400 is connected with the second steam pipe 700, and the second steam pipe 700 is arranged upstream of the filter B, and the fifth valve 710 is arranged on the second steam pipe 700. Preferably, the number of the fourth valve 810 and the fifth valve 710 is two, a double valve isolation system is adopted, double isolation is realized, and sterility is ensured. The fourth valve 810 and the fifth valve 710 are preferably diaphragm valves, and other types such as ball valves and butterfly valves can also be selected, which need to meet the requirements of sterility, corrosion resistance, easy cleaning and reliable sealing.

[0055] Further, the first fermenter E1 needs to be sterilized before fermentation, and in order to observe the pressure during sterilization, the first sterile water tank A1 is provided with the first pressure gauge F, and / or the first pipe 400 is provided with the second pressure gauge G, and / or the first fermenter E1 is provided with the third pressure gauge H. The first sterile water tank A1 is provided with the first exhaust valve 10, the first fermenter E1 is provided with the second exhaust valve 20, and the second pressure gauge G is provided with the third exhaust valve 30. The first exhaust valve 10 and the first pressure gauge F are connected by a pipe, the second exhaust valve 20 and the third pressure gauge H are connected by a pipe. The first exhaust valve 10, the second exhaust valve 20 and the third exhaust valve 30 are preferably diaphragm valves.

[0056] Further, in order to realize the on-off of the first air pipe 100-1 and facilitate sterilization, the sixth valve 110 and the seventh valve 120 are arranged on the first air pipe 100-1, the second ammonia water branch pipe 630 is arranged downstream of the sixth valve 110, and the seventh valve 120 is arranged downstream of the second steam pipe 700. The sixth valve 110 and the seventh valve 120 are preferably diaphragm valves, and other types such as ball valves and butterfly valves can also be selected, which need to meet the requirements of sterility, corrosion resistance, easy cleaning and reliable sealing.

[0057] Furthermore, to facilitate the connection and disconnection of the first sterile water tank A1 and the first air duct 100-1, and to facilitate sterilization, the first duct 400 is equipped with an eighth valve 410, a ninth valve 420, and a tenth valve 430. The eighth valve 410 is located between the first sterile water tank A1 and the first steam duct 800, and integrates a fourth exhaust valve 40. The ninth valve 420 is located between the water pump C and the spray head D, and the tenth valve 430 is located between the filter B and the water pump C. The first ammonia branch pipe 620 is located downstream of the tenth valve 430. The tenth valve 430 is preferably a diaphragm valve. The eighth valve 410, ninth valve 420, and tenth valve 430 are preferably diaphragm valves, but other types of valves can be selected according to actual needs. The eighth valve 410, ninth valve 420, and tenth valve 430 can be connected to the first duct 400 by clamps, flanges, or unions; the specific connection method is not specifically limited.

[0058] Furthermore, in order to ensure positive pressure between the first sterile water tank A1 and the first pipeline 400, a portion of sterile dry hot air is connected to the first sterile water tank A1 through the fourth pipeline 900. An eleventh valve 910 is provided on the fourth pipeline 900, and a fifth exhaust valve 50 is provided on the eleventh valve 910. Preferably, two fifth exhaust valves 50 are provided.

[0059] Furthermore, the second embodiment ( Figure 2 The specific components of the fermentation apparatus (shown) are described below: A second pipeline valve 210 is installed on the second pipeline 200, and a third pipeline valve 310 is installed on the third pipeline 300. The second sterile water tank A2 is preferably connected in parallel with the second air pipeline 100-2. A fifteenth valve 140 and a sixteenth valve 150 are installed on the parallel section of the second air pipeline 100-2. When it is necessary to cool and humidify the sterile dry hot air, the fifteenth valve 140 and the sixteenth valve 150 are closed, and the second pipeline valve 210 and the third pipeline valve 310 are opened. The dehumidified air is heated by heater I, passes through the primary filter J and the fine filter K, and is washed by the second sterile water tank A2 before entering the second fermentation tank E2. When no water replenishment is needed, the second pipeline valve 210 and the third pipeline valve 310 are closed, and the fifteenth valve 140 and the sixteenth valve 150 are opened. The sterile dry hot air enters the second fermentation tank E2 directly without passing through the second sterile water tank A2.

[0060] It should be noted that heater I, primary filter J, and fine filter K are all existing equipment in the fermentation unit. These components are used to sterilize the second fermenter E2, the second sterile water tank A2, and all pipes and valves. Figure 2The system shown in the embodiment is provided with a third steam pipeline 720 and a fourth steam pipeline 730, the third steam pipeline 720 is provided with a seventeenth valve 721, the fourth steam pipeline 730 is provided with an eighteenth valve 731, a twelfth valve 130 is arranged on the second air pipeline 100-2 and is arranged upstream of the third steam pipeline 720 to facilitate steam sterilization. The third steam pipeline 720 is arranged between the heater I and the primary filter J, and the fourth steam pipeline 730 is connected downstream of the second pipeline 200, and the specific connection position can be referred to Figure 2 To prevent the backflow of the sterile dry hot air into the second fermentation tank E2, a check valve 160 is arranged before the second air pipeline 100-2 enters the second fermentation tank E2. It should be noted that, in the embodiment, the second sterile water tank A2, the second pipeline 200, the third pipeline 300, the second pipeline valve 210, the third pipeline valve 310, the fifteenth valve 140 and the sixteenth valve 150 are equipment and valves added on the basis of the existing fermentation device. Figure 2

[0061] The fermentation device disclosed by the embodiment of the utility model takes the first fermentation tank E1 as an example to illustrate the specific working process: Figure 1

[0062] Firstly, before the first fermentation tank E1 is fed and sterilized, the first fermentation tank E1, the first sterile water tank A1, the water pump C, the filter B, each valve and the pipeline connection are completed, and at the same time, air pressure test is carried out, and each valve, pipeline and joint are qualified if no leakage is found.

[0063] ​​Secondly, the first fermentation tank E1 and the first sterile water tank A1 are sterilized, which can adopt the way of sterilizing both at the same time, or the way of sterilizing both separately. The method of sterilizing the first fermentation tank E1 and the first sterile water tank A1 at the same time is as follows: first, put the material into the first fermentation tank E1, then add water into the first sterile water tank A1, the specific water volume is determined according to the actual demand, open the fourth valve 810 on the first steam pipeline 800 and the fifth valve 710 on the second steam pipeline 700, sterilize the first fermentation tank E1 and the first sterile water tank A1 by steam warming, so that the temperature of the first fermentation tank E1 and the first sterile water tank A1 reaches 121-123℃, the pressure is 0.11-0.13Mpa for half an hour, to ensure that there is no dead angle and full sterilization, that is, all the pipelines for water and ammonia water supplement must be sterilized by steam flow for 1 hour, the pressure is maintained at 0.11-0.13Mpa, strictly according to the liquid deep fermentation industry standard manual, according to the requirements of sterile sterilization 121-123℃, pressure 0.11-0.13Mpa, to ensure that all pipelines, valves, filters B, etc. through which sterile water and air pass must be sterilized by high-temperature steam flow to reach 121℃ for 30 minutes, observe the pressure values of the first pressure gauge F, the second pressure gauge G and the third pressure gauge H, which should all reach 0.11-0.13Mpa, the sixth exhaust valve 60, the fifth exhaust valve 50, the fourth exhaust valve 40, the third exhaust valve 30 and the fifth valve 710 exhaust powerfully and obviously with part of blue color, to ensure that the temperature in the pipeline reaches 121℃, if the exhaust valves exhaust weakly in white mist, the sterilization is unqualified.

[0064] The sterilization process of the first fermenter El and the first sterile water tank Al is as follows: first, sterilize the first fermenter El, add the material according to the process requirements, and set the volume. According to the preset volume (usually half of the volume of the first fermenter El), set the volume and raise the temperature. Before sampling, check the temperature measuring element, PH measuring element, dissolved oxygen probe and display instrument to maintain normal state. When the temperature reaches 121℃, the pressure value of the third pressure gauge H reaches 0.1Mpa-0.13Mpa, start timing for 30 minutes, and open the ninth valve 420 in front of the spray head D, the third valve 631 of the ammonia supplement water, the second valve 621, and the sixth exhaust valve 60. Open the first steam pipeline 800, observe the second pressure gauge G to display the pressure of about 0.1Mpa, and the third exhaust valve 30 of the second pressure gauge G, the sixth exhaust valve 60 and the seventh exhaust valve 70 of the ammonia supplement three-way valve exhaust powerfully. When the first fermenter El is normally sterilized, close the ninth valve 420, the third valve 631, the second valve 621, the sixth exhaust valve 60 and the seventh exhaust valve 70, and open the first valve 611 at the same time. Let the ammonia water fill the three-way valve, ensure that all the peripheral water supplement valves and ammonia supplement valves connected with the first fermenter El are sterilized, then close the first fermenter El and other sampling valves, steam valves, etc. Finally, open the sixth valve 110 of the first air pipeline 100-1 and adjust the size of the inlet and outlet valves 110, 120 and the second exhaust valve 20 to the appropriate tank pressure.

[0065] Then sterilize the first sterile water tank Al. According to the fermentation process requirements, the sterilization can be delayed for several hours. According to the sterilization requirements of the deep fermentation tank, the temperature is 121℃, and the pressure is maintained for 30 minutes. When the first sterile water tank Al is heated, about 0.2Mpa steam flows through the filter B, the water pump C includes a half bypass valve 510 to display 0.1Mpa on the second pressure gauge G, and the sixth exhaust valve 60, the third exhaust valve 30 and the fourth exhaust valve 40 exhaust powerfully. When the temperature of the first sterile water tank Al reaches 121℃ and the first pressure gauge F displays 0.11MPa, the water supplement and ammonia supplement pipelines have been sterilized for half an hour. Then time for half an hour and ensure that the pressure of the first pressure gauge F, the second pressure gauge G and the third pressure gauge H displays 0.11Mpa-0.13Mpa, and the thermometer displays 121℃-123℃. Close the sixth exhaust valve 60, the third exhaust valve 30 and the fourth exhaust valve 40, then close the fourth valve 810, and immediately open the eighth valve 410 to fill the first pipeline 400 to the ninth valve 420 with high-temperature sterile water. Finally, open the eleventh valve 910 and adjust the first exhaust valve 10 to the stable tank pressure, then open the cooling water to reduce the temperature of the sterile water to below 30℃ for standby.

[0066] Finally, the water spraying operation is performed. When the fermentation reaches the water supplement requirement (usually 6-10 hours of fermentation, the dissolved oxygen decreases to below 50%), start water supplement. In special cases, water supplement can be started at the beginning of fermentation.

[0067] Open bypass valve 510 halfway, then start water pump C and observe that there are no abnormalities. Gradually close bypass valve 510. When the pointer of the second pressure gauge G rises to 0.5 MPa, open the ninth valve 420. It can be seen that the pressure of the second pressure gauge G drops to 0.3 MPa-0.4 MPa. Finally, close bypass valve 510 completely to stabilize the pressure of the second pressure gauge G at 0.5 MPa-0.6 MPa. If it is greater than 0.6 MPa or less than 0.5 MPa, adjust water pump C to stabilize the spray pressure at 0.5 MPa-0.6 MPa. At the same time, touch the first air pipe 100-1 after water replenishment. It should be noticeably cool, especially when switching to ammonia replenishment. Touch the air pipe and it should be icy cold, like 5℃-10℃.

[0068] During this period, keep detailed records, including recording pressure gauge data every 2 hours, checking the temperature of the first air pipe 100-1 by hand, and observing the liquid level in the first sterile water tank A1. According to the fermentation process requirements, water replenishment spraying should generally be stopped when the biomass, such as wet weight (OD value), or enzyme activity, no longer increases. For example, for saccharifying enzymes (Aspergillus niger), the normal cycle is 140-160 hours, and the enzyme activity is above 120,000-130,000 U. Water replenishment should generally be stopped 10 hours in advance, i.e., by stopping water pump C and closing valve 420 (ninth valve). After fermentation is complete and the tanks are empty, check that all valves and instruments are in good working order before waiting for the next batch of production.

[0069] To verify that the fermentation device disclosed in this utility model embodiment can increase dissolved oxygen and negative oxygen ions, the applicant conducted... Figure 2 The fermentation apparatus shown was experimentally verified. First, the dehumidified sterile air was indirectly heated to 60℃-70℃ with steam, filtered through the primary filter J (0.3μm) and the fine filter K (0.01μm), and then sterilized to obtain dry hot sterile air at 50℃-55℃ and a pressure of 0.08Mpa-0.09Mpa. The sterile dry hot air from one of the second fermentation tanks E2, at a rate of 5-10 liters / minute, was humidified and cooled by water washing in the second sterile water tank A2 to increase dissolved oxygen and negative oxygen ions. The other second fermentation tank E2 was used for a comparative experiment using dry hot air. Two 20-liter secondary fermenters E2 were sterilized with the same formula, and 10L of culture medium was added to each. Then, 2L of seed culture (from the same seed tank) was added to each of the two secondary fermenters E2. The seed culture cycle was 48 hours, and the viability was 19,000 u. The two secondary fermenters E2 were fermented simultaneously, with ammonia added to adjust the pH to 4.6-4.8 according to process requirements. Sugar syrup was added until the reducing sugar content in the fermentation broth reached 0.2%-0.5%, and the corresponding airflow rate and speed were maintained until fermentation was complete. One secondary fermenter E2 (a) was directly vented with hot dry air, while the other secondary fermenter E2 (b) was vented with cold, humid air after the dissolved oxygen level dropped to 50% (approximately 6 hours of fermentation). The air passed through the second sterile water tank A2 before entering the secondary fermenter E2. The experimental results are as follows:

[0070]

[0071] As shown in the table above, under the same fermentation time, the oxygen content in the exhaust gas of tank B is lower than that of tank A. The average oxygen content in the exhaust gas of tank A is 18.08%, while that of tank B is 17.79%. The maximum oxygen utilization rate in tank B is (20.6-16.3)% = 4.3%, and the oxygen utilization rate is increased by (18.08%-17.79%) / 4.3%×100%≈7%. Under the same fermentation time, the enzyme activity value in tank B is greater than that in tank A. The above experiments show that cooling and humidifying with sterile dry hot air can increase the dissolved oxygen content in the second fermenter E2, thereby increasing enzyme activity and fermentation efficiency. Furthermore, the fermentation device disclosed in this invention reduces costs compared to existing fermentation devices. Taking the above experiments as an example, preliminary estimates suggest that the total cost can be reduced by approximately 11%.

[0072] In addition, the applicant also through Figure 1 The fermentation apparatus shown was used in the experiment. After sterilization of the first fermentation tank E1 with a volume of 60 cubic meters, 30 cubic meters of base material was inoculated with 20% of the seed liquid (approximately 6 cubic meters) with a viability of 18,000 u. Fermentation was carried out according to the process requirements for 6 hours. When the dissolved oxygen level dropped to 30%-40%, cold water spraying was started at 40-50 liters / hour and a water pressure of over 0.4 MPa. The temperature of the sterile dry hot air dropped from 50℃-58℃ to 28℃-30℃ (the first air pipe 100-1 was very cool to the touch). The water replenishment time ended at 96 hours, with a total water replenishment of 2.8 cubic meters. At 48 hours of fermentation, when the first fermentation tank E1 was almost full, ammonia water could be introduced by water pump C spraying, and the pH range could be met. At this time, the eighth valve 410 was closed, and the cold water and ammonia water were mixed together by water pump C and sprayed through spray head D for cooling and humidification. Finally, the fermentation ended at 136-140 hours (a few hours earlier than the original dry hot air fermentation).

[0073] The control tank had an average monthly discharge volume of 61 cubic meters and an average activity of 101,300 u, with a total average of 618 standard tons (industry standard is 1 cubic meter = 1 standard ton for every 10,000 u of activity). The first fermentation tank E1, which was spray-hydrated, had an average discharge volume of 64 cubic meters and an average activity of 105,100 u, with a total output of 673 standard tons. The total enzyme activity increased by (673 - 618 / 618 * 100%) = 8.89%. Based on a rough estimate, the efficiency increased by about 30%.

[0074] As can be seen from the above data, the fermentation device disclosed in this utility model embodiment can increase the dissolved oxygen in the first fermentation tank E1 by spraying sterile dry hot air to cool and humidify, thereby improving enzyme activity and reducing costs.

[0075] At the same time, in combination with theoretical derivation, the beneficial effects of the fermentation device disclosed in the embodiment of the utility model can be obtained, that is, 2 grams of water per cubic meter of dry hot air containing 0.15Mpa, 50℃ is pressurized and sprayed with 20℃ cold water, so that the water content is 25 grams per cubic meter and the temperature is reduced to 30℃, and the air pressure is unchanged, wherein the content of dissolved oxygen is increased by about 22.6%, and the specific calculation process is as follows:

[0076] 1. Calculate the water vapor partial pressure

[0077] According to the ideal gas equation PV=nRT, wherein P is pressure (Pa), V is volume (m³), n is the amount of substance (mol), R is the gas constant (8.314 J / mol.k), and T is temperature (K),

[0078] The molar mass of water is 18g / mol, so 2g of water corresponds to 0.111mol, assuming the volume is 1m³ and the temperature is 50℃ (323.15K), then the water vapor partial pressure is P=nRT÷V=(0.111×8.314×323.15)÷1≈298.2pa

[0079] 25g of water corresponds to 1.389mol, and the water vapor partial pressure at 30℃ (303.15K) is P=(1.389×8.314×303.15)÷1≈3500.8pa,

[0080] 2. Determine the change of oxygen partial pressure

[0081] Initial state P O2 Initial=0.21×(150000-298.2)≈31437.4pa

[0082] Final state P O2 Final=0.21×(150000-3500.8)≈30764.8pa

[0083] 3. Calculate the change of dissolved oxygen: according to Henry's law: C=KH×P O2

[0084] Initial dissolved oxygen concentration Cinitial=1.2×10 -3 ×0.314≈0.3768×10 -3 mol / L

[0085] Final dissolved oxygen concentration Cinitial=1.5×10 -3 ×0.308≈0.462×10 -3 mol / L

[0086] Increase ΔC=Cfinal-Cinitial=0.0852×10 -3mol / L, the increasing rate AC / Cinitial*100%=(0.0852*10 -3 )÷(0.3768*10 -3 )×100%=22.6%.

[0087] From the above calculation, it can be seen that the fermentation device disclosed in the embodiment of the utility model, through the way of spraying humidification, can improve the content of dissolved oxygen in sterile dry hot air.

[0088] The fermentation device disclosed in the embodiment of the utility model is suitable for deep liquid fermentation devices which need a large amount of oxygen for high-intensity fermentation, and the water supply amount and air volume can be adjusted according to the strain process and equipment difference, so that the effect of increasing dissolved oxygen and improving fermentation efficiency can be achieved.

[0089] It should be noted that each embodiment in the specification adopts a progressive manner for description, and each embodiment focuses on the difference from other embodiments, and the same and similar parts between each embodiment can be referred to.

[0090] Hereinafter, the terms "first" and "second" are only for description purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features defined with "first" and "second" can explicitly or implicitly include one or more features.

[0091] In this paper, "and / or" is only a description of the association relationship between the associated objects, which means that there can be three relationships, for example, A and / or B, which can represent three cases: A exists alone, A and B exist together, and B exists alone.

[0092] In this paper, specific examples are applied to describe the principles and implementation modes of the utility model, and the above embodiment description is only used to help understand the core idea of the utility model. It should be pointed out that for ordinary skilled persons in the technical field, without departing from the principles of the utility model, the utility model can be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the utility model claims.

Claims

1. A fermentation apparatus, characterized by, The application relates to a fermentation system, which comprises: a fermentation tank (E) connected with an air pipeline (100), wherein the fermentation tank (E) is provided with a stirring mechanism (E3); a sterile water tank (A) connected with the air pipeline (100) through a first pipeline (400), wherein a spraying head (D) is arranged on the first pipeline (400); or the air pipeline (100) is connected with the sterile water tank (A) through a second pipeline (200), and the sterile water tank (A) is connected with the air pipeline (100) through a third pipeline (300).

2. The fermentation device of claim 1, wherein, The fermentation tank (E) comprises a first fermentation tank (E1) or a second fermentation tank (E2), the air pipeline (100) comprises a first air pipeline (100-1) or a second air pipeline (100-2), the sterile water tank (A) comprises a first sterile water tank (A1) or a second sterile water tank (A2), the first fermentation tank (E1) is connected with the first air pipeline (100-1), the first sterile water tank (A1) is connected with the first air pipeline (100-1) through the first pipeline (400), and the spraying head (D) is arranged on the first pipeline (400); or the second fermentation tank (E2) is connected with the second air pipeline (100-2), the second air pipeline (100-2) is connected with the second sterile water tank (A2) through the second pipeline (200), and the second sterile water tank (A2) is connected with the second air pipeline (100-2) through the third pipeline (300).

3. The fermentation device of claim 2, wherein, A water pump (C) is arranged on the first pipeline (400) and downstream of the first sterile water tank (A1), the first pipeline (400) is provided with a bypass pipe (500) connected with the water pump (C) in parallel, and a bypass valve (510) is arranged on the bypass pipe (500).

4. The fermentation device of claim 3, wherein, An ammonia water pipeline (600) is further arranged, the ammonia water pipeline (600) comprises an ammonia water main pipe (610) and a first ammonia water branch pipe (620) and a second ammonia water branch pipe (630) connected with the ammonia water main pipe (610) respectively, the first ammonia water branch pipe (620) is connected with the first pipeline (400), and the second ammonia water branch pipe (630) is connected with the first air pipeline (100-1).

5. The fermentation device of claim 4, wherein, A first valve (611) is arranged on the ammonia water main pipe (610), a second valve (621) is arranged on the first ammonia water branch pipe (620), and a third valve (631) is arranged on the second ammonia water branch pipe (630).

6. The fermentation device of claim 5, wherein, A filter (B) is arranged on the first pipeline (400) and between the first sterile water tank (A1) and the water pump (C).

7. The fermentation device of claim 6, wherein, The first air pipeline (100-1) is connected with a first steam pipeline (800), and a fourth valve (810) is arranged on the first steam pipeline (800). The first pipeline (400) is communicated with a second steam pipeline (700), the second steam pipeline (700) is arranged upstream of the filter (B), and the second steam pipeline (700) is provided with a fifth valve (710).

8. The fermentation device of claim 7, wherein, The first sterile water tank (A1) is provided with a first pressure gauge (F); and / or, The first pipeline (400) is provided with a second pressure gauge (G); and / or, The first fermentation tank (E1) is provided with a third pressure gauge (H).

9. The fermentation device of claim 8, wherein, The first air pipeline (100-1) is provided with a sixth valve (110) and a seventh valve (120), the second ammonia water branch pipeline (630) is arranged downstream of the sixth valve (110), and the seventh valve (120) is arranged downstream of the second steam pipeline (700).

10. The fermentation device of claim 7, wherein, The first pipeline (400) is provided with an eighth valve (410), a ninth valve (420) and a tenth valve (430), the eighth valve (410) is arranged between the first sterile water tank (A1) and the first steam pipeline (800), the ninth valve (420) is arranged between the water pump (C) and the spray head (D), the tenth valve (430) is arranged between the filter (B) and the water pump (C), and the first ammonia water branch pipeline (620) is arranged downstream of the tenth valve (430).

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