Micro-fluidic chip for nucleic acid detection
By designing the structure of a microfluidic chip and using pre-encapsulated primer freeze-drying technology, the problems of multiple pipetting and high contamination risk in existing nucleic acid testing have been solved, achieving the effects of simplified operation and reduced equipment costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-05
- Publication Date
- 2026-03-27
AI Technical Summary
Existing nucleic acid testing methods require multiple pipetting operations, making accurate quantification difficult and posing a high risk of contamination. The equipment is also complex and costly.
Design a microfluidic chip comprising a chip body, a puncture structure, a reaction chamber, and connecting tubing. Pre-encapsulated primers are lyophilized in a reagent package to simplify the operation steps. Reagents are injected through the puncture structure and nucleic acid amplification reaction is performed in the reaction chamber. The flow of reagents is controlled by an exhaust mechanism, eliminating the need for a valve structure.
It simplifies the operation process, reduces the risk of contamination, reduces reliance on expensive equipment, and improves the portability and sensitivity of the detection.
Smart Images
Figure CN224047375U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to nucleic acid detection technical field especially relates to a micro -fluidic chip for nucleic acid detection. BACKGROUND
[0002] At present, in the nucleic acid detection process, the collected nucleic acid sample needs to at least pass through the following steps: lysis extraction nucleic acid, and mixes with nucleic acid amplification reagent (such as enzyme etc.), primer and carries out amplification. One of the more convenient detection methods is to complete all the above steps in a micro -fluidic chip, but it needs to set more chambers in the chip to store lysis and neutralization liquid and other nucleic acid extraction reagents, or provide the space of nucleic acid sample and the mixing reaction of these reagents. In addition, it also needs to set piston, valve and the like on the chip to control the mixing, flow and the like of sample and reagent. This makes the structure of micro -fluidic chip more complex, and the nucleic acid analyzer matched with it also needs to set the driving mechanism for driving these pistons, valves, so that the whole detection system is more complex, and the equipment cost is higher. The traditional manual test method needs to be pipetted for many times, is difficult to accurately quantitatively pipette or needs to use expensive pipette gun, and the pollution risk is higher, the nucleic acid detection has the characteristics of integration, and also has the characteristics of high sensitivity, high specificity detection of system, the reagent after nucleic acid extraction and purification is pre-packaged in reagent bag and integrated on a micro -fluidic chip, which simplifies the operation steps, is convenient and fast, greatly simplifies the human operation, realizes the portable function of simple device, and on this basis, the matched instrument can detect three micro -fluidic chips as a control at the same time. SUMMARY
[0003] The utility model aims at solving the prior art problems, and proposes a micro -fluidic chip for nucleic acid detection.
[0004] In order to realize the above-mentioned purpose, the utility model adopts the following technical scheme:
[0005] A micro -fluidic chip for nucleic acid detection, including chip main part, be provided with opening on the chip main part, the lower portion of opening is provided with reagent inlet, be provided with puncture structure in the opening, the puncture structure is provided with connecting hole through, the connecting hole is linked with reagent inlet, the reagent inlet is connected with amplification reaction area through connecting pipeline, the amplification reaction area includes several reaction bins, the connecting pipeline includes input pipe and several output pipes, the one end of input pipe is linked with reagent inlet, the other end of input pipe is linked with several output pipes, and the other end of several output pipes is linked with corresponding reaction bin.
[0006] Preferably, the puncture structure is a sharp member, and the sharp member is arranged in the opening.
[0007] Preferably, the reaction chamber is provided with pre-stored materials, which are pre-packaged primer lyophilized.
[0008] Preferably, the input pipe is provided with an exhaust mechanism, which comprises exhaust holes, waterproof and breathable membranes and a sealing cover.
[0009] Preferably, the exhaust hole is cylindrical, the side of the waterproof and breathable membrane is circular, and the exhaust hole is matched with the waterproof and breathable membrane.
[0010] Preferably, the opening is cylindrical.
[0011] Preferably, the reaction chambers are arranged in a rectangular array in the chip body.
[0012] Compared with the prior art, the utility model has the beneficial effects that:
[0013] The utility model discloses a chip body, in use, the reagent bag can be through the puncture structure and make the reagent in the reagent bag flow into the reagent entrance through the connecting hole, then flow into the chip body through the reagent entrance, so that the reagent flows into the reaction chamber through the input pipe and the output pipe, and starts to react with other pre-stored materials in the reaction chamber, forms nucleic acid amplification reaction, then, through the external detection module detection can, compared with the prior art, the reaction chamber is arranged, and the reagent after nucleic acid extraction and purification is pre-packaged in the reagent bag and integrated on a microfluidic chip with detection, which simplifies the operation steps, solves the problems of the test method needing multiple pipetting, difficult accurate quantitative pipetting or needing to use expensive pipette gun in the prior art, and higher pollution risk. BRIEF DESCRIPTION OF DRAWINGS
[0014] Fig. 1 It is an overall schematic view of a microfluidic chip for nucleic acid detection according to the utility model;
[0015] Fig. 2 It is a top view of a microfluidic chip for nucleic acid detection according to the utility model.
[0016] In the figure: 1, chip body;2, reagent bag;3, opening;4, reagent entrance;5, reaction chamber;6, input pipe;7, output pipe;9, sharp part;10, exhaust hole;11, waterproof and breathable membrane;12, sealing cover. DETAILED DESCRIPTION
[0017] Clearly, the described embodiments are merely a part of the embodiments of the present application, but not all the embodiments.
[0018] With reference to Figs. 1-2 A microfluidic chip for nucleic acid detection, comprising a chip body 1, an opening 3 is arranged on the chip body 1, a reagent inlet 4 is arranged below the opening 3, a puncture structure is arranged in the opening 3, a connecting hole is arranged through the puncture structure, the connecting hole is in communication with the reagent inlet 4, the reagent inlet 4 is connected with an amplification reaction zone through a connecting pipeline, the amplification reaction zone comprises a plurality of reaction bins 5, the connecting pipeline comprises an input pipe 6 and a plurality of output pipes 7, one end of the input pipe 6 is in communication with the reagent inlet 4, the other end of the input pipe 6 is in communication with the plurality of output pipes 7, and the other end of the plurality of output pipes 7 is in communication with the corresponding reaction bin 5.
[0019] In use, the reagent bag 2 can flow into the reagent inlet 4 through the connecting hole by the puncture structure, and then flow into the chip body 1 through the reagent inlet 4, so that the reagent flows into the reaction bin 5 through the input pipe 6 and the output pipe 7, and reacts with the pre-stored other materials in the reaction bin 5 to form a nucleic acid amplification reaction, and then is detected by an external detection module. Compared with the prior art, the reaction bin 5 is arranged, the reagent after nucleic acid extraction and purification is pre-packaged in the reagent bag, and detection is integrated on a microfluidic chip, which simplifies the operation steps, solves the problem that the test method in the prior art needs to be pipetted multiple times, it is difficult to accurately pipette or needs to use an expensive pipette gun, and the pollution risk is high.
[0020] Further, the puncture structure is a sharp piece 9, the sharp piece 9 is arranged in the opening 3, and the reagent bag 2 arranged outside can be pierced by the sharp piece 9, so that the reagent can flow into the reagent inlet 4 through the connecting hole.
[0021] Further, the reaction bin 5 is provided with pre-stored materials, the pre-stored materials are pre-packaged primer lyophilization, and the pre-stored materials can react with the materials flowing into the reaction bin 5, so that the operation steps can be simplified.
[0022] Further, the exhaust mechanism comprises a plurality of exhaust holes 10, a plurality of waterproof air permeable membranes 11 and a sealing cover 12, the plurality of exhaust holes 10 are arranged on the corresponding output pipe 7, the plurality of waterproof air permeable membranes 11 are arranged in the corresponding exhaust hole 10, and the sealing cover 12 is arranged on the chip body 1 and connected with the exhaust hole 10. Through the arrangement of the plurality of exhaust holes 10, the plurality of waterproof air permeable membranes 11 and the sealing cover 12, the sealing cover 12 can be selectively bent in use, so that the exhaust hole 10 is closed, so that the flow of the reagent can be controlled in use, and a valve for controlling the liquid does not need to be arranged, so that the use is more convenient. Through the arrangement of the waterproof air permeable membrane 11, the reagent can be prevented from being discharged from the exhaust hole 10, so that the exhaust hole 10 can only discharge gas.
[0023] Further, the exhaust hole 10 is in a cylindrical shape, the side of the waterproof air permeable membrane 11 is circular, and the exhaust hole 10 is matched with the waterproof air permeable membrane 11. Through the arrangement of the exhaust hole 10 in a cylindrical shape, the exhaust hole 10 can be conveniently sealed by the sealing cover 12. Through the arrangement of the side of the air permeable membrane in a circular shape, the air permeable membrane can be matched with the exhaust hole 10, so that the reagent is prevented from flowing out.
[0024] Further, the opening 3 is in a cylindrical shape. Through the arrangement of the opening 3 in a cylindrical shape, the reagent bag 2 can be conveniently put into the opening 3, and the reagent bag 2 can be conveniently pierced by the sharp member 9, so that the use is more convenient.
[0025] Further, the plurality of reaction bins 5 are arranged in a rectangular array in the chip body 1. Through the arrangement of the reaction bin 5 in a rectangular array, the reagent can be uniformly flowed into the corresponding reaction bin 5.
[0026] In use, the reagent bag can be put into the opening 3, the sharp member 9 arranged in the opening 3 can be pierced into the reagent bag 2 by pressing the reagent bag 2, the reagent can be flowed into the reagent inlet 4 through the connecting hole, and then flowed into the chip body 1 through the reagent inlet 4, so that the reagent can be flowed into the reaction bin 5 through the input pipe 6 and the output pipe 7, and react with the pre-stored material in the reaction bin 5 to form a nucleic acid amplification reaction. If it is necessary to control the flow of the reagent, the sealing cover 12 can be selectively bent, so that the exhaust hole 10 is closed, so that the flow of the reagent can be controlled in use, and a valve for controlling the liquid does not need to be arranged to control the flow of the reagent, so that the use of the device is convenient.
[0027] The above merely describes a preferred specific implementation of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can make equivalent replacements or changes according to the technical scheme and the inventive concept of the present application within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.
Claims
1. A microfluidic chip for nucleic acid detection comprising a chip body (1), characterized in that: The chip body (1) is provided with an opening (3), and the opening (3) is provided with a reagent inlet (4) below; a puncture structure is arranged in the opening (3), and a connecting hole is arranged through the puncture structure and communicates with the reagent inlet (4); the reagent inlet (4) is connected with an amplification reaction area through a connecting pipeline; the amplification reaction area comprises a plurality of reaction bins (5); the connecting pipeline comprises an input pipe (6) and a plurality of output pipes (7); one end of the input pipe (6) communicates with the reagent inlet (4); the other end of the input pipe (6) communicates with the plurality of output pipes (7); and the other end of the plurality of output pipes (7) communicates with the corresponding reaction bins (5). 2.The microfluidic chip for nucleic acid detection according to claim 1, wherein: The puncture structure is a sharp piece (9) arranged in the opening (3). 3.The microfluidic chip for nucleic acid detection according to claim 1, wherein: The reaction bin (5) is provided with pre-stored materials, and the pre-stored materials are pre-packaged primer lyophilized.
4. The microfluidic chip for nucleic acid detection according to claim 1, wherein: A plurality of input pipes (6) are provided with exhaust mechanisms, and the exhaust mechanisms comprise a plurality of exhaust holes (10), a plurality of waterproof air permeable membranes (11) and a sealing cover (12); the plurality of exhaust holes (10) are arranged on the corresponding output pipes (7); the plurality of waterproof air permeable membranes (11) are arranged in the corresponding exhaust holes (10); and the sealing cover (12) is arranged on the chip body (1) and connected with the exhaust holes (10).
5. The microfluidic chip for nucleic acid detection according to claim 4, wherein: The exhaust hole (10) is in a cylindrical shape, the side surface of the waterproof air permeable membrane (11) is circular, and the exhaust hole (10) is matched with the waterproof air permeable membrane (11).
6. The microfluidic chip for nucleic acid detection according to claim 1, wherein: The opening (3) is in a cylindrical shape.
7. The microfluidic chip for nucleic acid detection according to claim 1, wherein: A plurality of reaction bins (5) are arranged in a rectangular array in the chip body (1).