Sample nucleic acid extraction equipment

Abstract

The utility model provides sample nucleic acid extraction equipment. Comprising a storage device, a transfer device, a liquid separation device for automatically dispensing a reagent into a to-be-treated sample, and a centrifugal device for carrying out centrifugal treatment on a mixture of the sample and the reagent, the storage device is used for centrifuging a sample plate, the temperature control device is used for carrying out temperature control treatment on the centrifuged sample plate, the extraction device is used for carrying out nucleic acid extraction treatment on the sample after temperature control treatment, the storage device comprises a first storage part and a second storage part, the first storage part is used for storing the sample to be treated, and the second storage part is used for storing consumables and required reagent materials; the first storage part, the liquid separation device, the centrifugal device, the temperature control device and the extraction device are located within the transfer range of the transfer device, and the transfer device is used for automatically transferring samples, consumables and reagent materials at least among the first storage part, the liquid separation device, the centrifugal device, the temperature control device and the extraction device. The sample nucleic acid extraction equipment can effectively save labor and improve efficiency.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of medical equipment, concretely relates to a sample nucleic acid extraction equipment. BACKGROUND

[0002] The operation mode of the existing CRC, Meta, WGS and other extraction procedures is relatively backward, manual dispensing, film sealing, film tearing and sample transfer consume time and effort, and manual feeding and discharging are needed, and manual sample, material and consumable transfer is needed, the personnel operation proficiency is closely related to the quality of the post, quality accidents are prone to occur, and there are technical problems of low efficiency, large manual operation workload and error risk.

[0003] Therefore, to meet the market demand, it is urgent to improve the existing preparation mode to save labor cost and improve the efficiency of sample extraction. UTILITY MODEL CONTENT

[0004] The utility model mainly solves the technical problem that a sample nucleic acid extraction equipment is provided to save labor, improve efficiency and reduce the working strength of experimenters.

[0005] The scheme for realizing the technical purpose of the utility model is a sample nucleic acid extraction equipment, which comprises:

[0006] The storage device comprises a first storage part and a second storage part, the first storage part is used for placing samples to be treated, and the second storage part is used for storing consumables and required reagent materials;

[0007] The dispensing device is used for automatically dispensing reagents into the samples to be treated;

[0008] The centrifugal device is used for centrifugal treatment of the mixture of the sample and the reagent;

[0009] The temperature control device is used for temperature control treatment of the sample plate after centrifugation;

[0010] The extraction device is used for nucleic acid extraction treatment of the sample after temperature control treatment;

[0011] The first storage part, the dispensing device, the centrifugal device, the temperature control device and the extraction device are located within the transfer range of the transfer device, and the transfer device is used for automatic transfer of the sample, the consumables and the reagent materials between the first storage part, the dispensing device, the centrifugal device, the temperature control device and the extraction device.

[0012] In an alternative embodiment, the transfer device comprises a track, a mechanical arm slidingly installed on the track, and a driving mechanism drivingly connected to the mechanical arm, the driving mechanism driving the mechanical arm to move along the track, the first storage part, the second storage part, the distribution device, the centrifugal device, the temperature control device, and the extraction device being distributed on at least one side of the track.

[0013] In an alternative embodiment, the track is a straight track, the first storage part, the second storage part, the distribution device, the centrifugal device, the temperature control device, and the extraction device being distributed on opposite sides of the track.

[0014] In an alternative embodiment, the extraction device is in a plurality, the plurality of extraction devices being arranged on the same side of the track and sequentially arranged along the length direction of the track.

[0015] In an alternative embodiment, the storage device comprises a storage mechanism and an interactive shelf, the storage mechanism having a containing cavity, the containing cavity constituting the second storage part; the first storage part is provided on the interactive shelf, and at least the interactive shelf is located within the transfer range of the transfer device.

[0016] In an alternative embodiment, the sample nucleic acid extraction apparatus further comprises a film tearing device, the film tearing device being used for film tearing treatment on the film-covered well plate, the film tearing device being located within the transfer range of the transfer device.

[0017] And / or; the sample nucleic acid extraction apparatus further comprises a film sealing device, the film sealing device being used for automatically sealing the wells of the well plate containing the sample, the film sealing device being located within the transfer range of the transfer device.

[0018] In an alternative embodiment, the storage mechanism and the interactive shelf are both located within the transfer range of the transfer device.

[0019] The sample nucleic acid extraction apparatus comprises a film tearing device and a film sealing device; the interactive shelf, the centrifugal device, the film tearing device, the film sealing device, and the temperature control device are distributed on a first side of the track in the width direction, the storage mechanism and the plurality of extraction devices are provided on a second side of the track in the width direction, the first side being opposite to the second side.

[0020] In the moving direction of the mechanical arm along the track, the interactive shelf, the centrifugal device, the film tearing device, and the film sealing device are arranged in a row, and the storage mechanism and the plurality of extraction devices are arranged in a row adjacent to each other.

[0021] In an alternative embodiment, the sample nucleic acid extraction apparatus further comprises a base, the interactive shelf, the centrifugal device, the extraction device, the track, the temperature control device, the film tearing device, and the film sealing device all being installed on the top end of the base.

[0022] In an alternative embodiment, the base has a hollow chamber with an opening facing the side of the base, the top surface of the base is provided with a through hole in communication with the hollow chamber, and the sample nucleic acid extraction device further comprises a collection mechanism, which is detachably installed in the hollow chamber, and the waste plate and consumables can be put into the collection mechanism through the through hole.

[0023] In an alternative embodiment, the sample nucleic acid extraction device further comprises a capping piece, the placement device comprises a first support, the first support is provided with a first positioning part matched with the capping piece, the capping piece is placed on the first support through the first positioning part, the first support is within the transfer range of the transfer device, and the capping piece can be transferred from the transfer device to the temperature control device, so that the capping piece can be placed on the sample plate with a film when the temperature control device controls the temperature of the sample plate after centrifugation.

[0024] In an alternative embodiment, the sample nucleic acid extraction device further comprises a reagent tool for centrifugal balancing, the placement device further comprises a second support, the second support is provided with a second positioning part matched with the reagent tool, the capping piece is placed on the second support through the second positioning part, and the second support is within the transfer range of the transfer device, and the reagent tool can be transferred between the centrifugal device and the second support by the transfer device.

[0025] According to the sample nucleic acid extraction device provided in the above embodiments, the sample nucleic acid extraction device can be used for extraction and purification of a 96-well plate sample, has the functions of carrying, centrifugation, pipetting, temporary storage and extraction, can automatically complete the carrying, centrifugation, pipetting, temporary storage and extraction of the well plate, reagents and consumables, can effectively reduce the work intensity of manual work, can improve the pipetting quality of automatic pipetting, can eliminate the risk of adding too much or missing adding reagents, can effectively reduce the error rate and improve the work efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 The overall schematic view of the sample nucleic acid extraction device provided in some embodiments of the utility model.

[0027] Figure 2 The arrangement schematic view of the temperature control device and the interactive shelf in Figure 1

[0028] Figure 3 The arrangement schematic view of the film sealing device, the film tearing device and the first support in Figure 1

[0029] ​​Reference signs: 10 - storage device; 11 - storage mechanism; 111 - connecting block; 12 - interactive frame; 13 - limiting angle block; 14 - first support; 15 - second support; 20 - liquid separation device; 30 - centrifugal device; 40 - temperature control device; 50 - extraction device; 60 - transfer device; 71 - film tearing device; 72 - film sealing device; 73 - buffer; 74 - proximity switch; 80 - base; 81 - through hole; 82 - base; 92 - material guide; 93 - code scanning gun; 94 - capping piece; 95 - protective plate. DETAILED DESCRIPTION

[0030] The utility model will be described in further detail below through specific implementation modes combined with drawings. Similar elements in different implementation modes adopt relevant similar element signs. In the following implementation modes, many detailed descriptions are for the sake of better understanding of the present application. However, those skilled in the art can easily realize that part of the features can be omitted in different cases or can be replaced by other elements, materials or methods. In some cases, some operations related to the present application are not shown or described in the specification, so as to avoid the core part of the present application from being overwhelmed by too much description, and it is not necessary for those skilled in the art to describe these related operations in detail according to the description in the specification and general technical knowledge in the art to completely understand the related operations.

[0031] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate way to form various implementation modes. At the same time, the steps or actions in the method description can also be sequentially changed or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for the purpose of clearly describing a certain embodiment, and do not mean that the sequence is necessary, unless otherwise stated that a certain sequence must be followed.

[0032] The serial numbers of components in this paper, such as "first", "second", etc., are only used to distinguish the described objects, and do not have any sequence or technical meaning. Unless otherwise specified, the "connection" and "coupling" in this application include direct and indirect connection (coupling).

[0033] The existing CRC, Meta, WGS and other blood sample nucleic acid extraction procedures have a relatively backward operation mode, among which manual liquid separation, film sealing and film tearing are time-consuming and labor-intensive, manual loading and unloading and manual code scanning are prone to errors, and it is urgent to upgrade them to a more automated device to save labor and improve efficiency.

[0034] To solve the above technical problems, the utility model provides a sample nucleic acid extraction equipment, please refer to Figures 1-3The sample nucleic acid extraction device includes a placement device 10, a dispensing device 20, a centrifugal device 30, a temperature control device 40, an extraction device 50, and a transfer device 60. The placement device 10 includes a first placement part and a second placement part. The first placement part is used to place samples to be processed. The second placement part is used to store consumables and required reagent materials. The dispensing device 20 is used to automatically dispense reagents into the samples to be processed. The centrifugal device 30 is used to centrifugally process the mixture of the samples and the reagents. The temperature control device 40 is used to control the temperature of the sample plate after centrifugation. The extraction device 50 is used to extract nucleic acids from the samples after temperature control. The first placement part, the dispensing device 20, the centrifugal device 30, the temperature control device 40, and the extraction device 50 are within the transfer range of the transfer device 60. The transfer device 60 is used to automatically transfer the samples, consumables, and reagent materials between at least the first placement part, the dispensing device 20, the centrifugal device 30, the temperature control device 40, and the extraction device 50.

[0035] According to the sample nucleic acid extraction device of the above-mentioned embodiments, the device can be used for the extraction and purification of 96-well plate samples. The temperature control mechanism is suitable for the pretreatment of blood samples that need to be subjected to temperature bath, i.e., enzyme reaction. The sample nucleic acid extraction device has the functions of sample handling, centrifugation, pipetting, temperature bath, and nucleic acid extraction. The device can automatically complete the handling, centrifugation, pipetting, temperature bath, and extraction of the well plate, reagents, and consumables, thereby effectively reducing the work intensity of the human labor, improving the pipetting quality through automatic pipetting, eliminating the risk of adding too much or missing reagents, and effectively reducing the error rate and improving the work efficiency.

[0036] In addition, the function of the temperature control device 40 can also be adaptively adjusted and selected according to actual needs. For example, in some embodiments, the temperature control device 40 can only be used to heat the samples and stabilize the temperature of the samples within a required range. In other embodiments, in addition to the function of heating the sample plate, the temperature control device 40 can also have the function of accelerating the cooling of the sample plate to room temperature, so as to effectively shorten the time of the entire fecal sample lysis incubation process.

[0037] In some embodiments, please refer to Figures 1-3 The transfer device 60 includes a track, a mechanical hand, and a driving mechanism. The mechanical hand is slidingly installed on the track. The driving mechanism is drivingly connected to the mechanical hand. The driving mechanism drives the mechanical hand to move along the track. The first placement part, the second placement part, the dispensing device 20, the centrifugal device 30, the temperature control device 40, and the extraction device 50 are distributed on at least one side of the track. On the one hand, by providing the track and enabling the mechanical hand to move along the track, the transfer range of the mechanical hand can be expanded. On the other hand, by arranging the placement device 10, the centrifugal device 30, the dispensing device 20, the temperature control device 40, and the extraction device 50 around the track, the volume of the sample nucleic acid extraction device can be controlled, and the device is convenient for users to use.

[0038] The number of tracks and mechanical hands can be adjusted according to requirements, and the utility model is not specifically limited. In some embodiments, referring to Figure 1 and Figure 2 , the track and the mechanical hand can be used in pairs, that is, one track is provided with one mechanical hand. In other embodiments, at least two mechanical hands can be provided on one track.

[0039] In some embodiments, referring to Figure 1 and Figure 2 , the mechanical hand can include a six-axis mechanical arm and an electric gripper. In order to increase the clamping area to stabilize the clamping of the sample plate, the reagent plate and the consumables, a clamping block can also be provided on the electric gripper.

[0040] The selection of the driving assembly can be any feasible technical solution, such as belt drive, screw drive, etc., and the utility model is not limited. In some embodiments, referring to Figure 2 and Figure 3 , the driving assembly can include a driving motor, a speed reducer and matching rack and pinion. The pinion is driven by the driving motor and the speed reducer, so that the mechanical hand can move along the rack and the track.

[0041] In some embodiments, referring to Figure 1 and Figure 2 , in order to transfer device 60 can also include an oil pump and an oil felt wheel connected by an oil circuit. The oil pump and the oil felt wheel move along the track with the mechanical hand. The oil felt wheel and the pinion are both provided on the mechanical hand and engaged with the rack. The oil pump can pump oil to the oil felt wheel to lubricate the rack during the movement of the mechanical hand, thereby ensuring that the mechanical hand can move smoothly along the track and effectively prevent it from being stuck.

[0042] In some embodiments, referring to Figures 1-3 , the track is a straight track, so that the mechanical hand can have a larger transfer range. The first storage part, the second storage part, the dispensing device 20, the centrifugal device 30, the temperature control device 40 and the extraction device 50 are distributed on opposite sides of the track. On the basis of the straight track, by reasonably distributing the layout of each mechanism and each device, the mechanical hand can automatically transfer the hole plate containing the sample to the next automatic process.

[0043] In some embodiments, referring to Figure 1 and Figure 3 , the number of extraction devices 50 is multiple, which can simultaneously extract multiple blood samples, which is beneficial to improve the work efficiency. The multiple extraction devices 50 are arranged on the same side of the track and sequentially arranged along the length direction of the track. The length direction of the track is the moving direction of the mechanical hand along the track. The multiple extraction devices 50 are arranged in a row, so that the space layout is more rationalized, and the overall volume of the sample nucleic acid extraction equipment can be effectively controlled.

[0044] In some embodiments, please refer to Figures 1-3 , the storage device 10 includes a storage mechanism 11 and an interactive rack 12, the storage mechanism 11 has a containing cavity, the containing cavity constitutes a second storage part; the first storage part is arranged on the interactive rack 12 for the operator to load, and at least the interactive rack 12 is located within the transfer range of the transfer device 60. The transfer device 60 can automatically transfer the sample plate to the dispensing device 20, and after completing the final processing, the transfer device 60 can also automatically transfer the sample plate after the extraction process is completed to the interactive rack 12, waiting for the worker to manually unload to perform subsequent detection processing.

[0045] In some embodiments, in order to reduce the error rate of the automatic process, the interactive rack 12 can be provided with a loading area and an unloading area.

[0046] The specific structure of the storage mechanism 11 is not limited in this embodiment, and in some embodiments, the storage mechanism 11 can be a fixed rack structure, and the taking out and placing of the hole plate and consumables are directly realized by the transfer device 60. In some embodiments, the storage mechanism 11 can also have the function of automatic unloading, and can adjust the required consumables or reagent plate and wait for the mechanical hand to clamp.

[0047] In some embodiments, please refer to Figures 1-3 , the first storage part can be a placement space defined by four limiting corner blocks 13, and a sample plate is positioned by the four limiting corner blocks 13. Since the four corners of the sample plate are only limited, the two opposite sides of the sample plate can be exposed to facilitate the mechanical hand to clamp the sample plate.

[0048] In some embodiments, in order to improve the automation degree of the equipment and reduce the error probability, the sample nucleic acid extraction equipment further includes a plurality of detection sensors, one detection sensor is arranged in one first storage part, and is used to detect whether a sample plate is placed in the first storage part.

[0049] In some embodiments, please refer to Figure 1 and Figure 3 , the interactive rack 12 is provided with a protective plate 95 for protecting the detection sensor, which can effectively protect the detection sensor and prolong the service life of the detection sensor.

[0050] In some embodiments, please refer to Figure 1 and Figure 3The sample nucleic acid extraction device further comprises a film tearing device 71 configured to tear the film of the covered well plate to expose the deep well and the liquid in the deep well. The film tearing device 71 is located in the transfer range of the transfer device 60. The film tearing of the well plate is performed by the automatic device instead of manual operation, which is beneficial to improve the efficiency and reduce the error rate. The transfer device 60 automatically transfers the sample between the devices and mechanisms, which can greatly improve the efficiency, reduce the manual workload, and ensure the yield.

[0051] In some embodiments, the sample nucleic acid extraction device further comprises a film sealing device 72 configured to automatically seal the well of the well plate containing the sample. The film sealing device 72 is located in the transfer range of the transfer device 60. The film tearing of the well plate is performed by the automatic device instead of manual operation, which is beneficial to improve the efficiency and reduce the error rate. The transfer device 60 automatically transfers the sample between the devices and mechanisms, which can greatly improve the efficiency, reduce the manual workload, and ensure the yield.

[0052] In some embodiments, the sample nucleic acid extraction device can simultaneously comprise the film tearing device 71 and the film sealing device 72 to realize the fully automatic film sealing and film tearing operations by the automatic device, which is beneficial to improve the efficiency and reduce the error rate.

[0053] In some embodiments, please refer to Figures 1-3 The storage mechanism 11 and the interaction rack 12 are located in the transfer range of the transfer device 60. The sample nucleic acid extraction device comprises the film tearing device 71 and the film sealing device 72. The interaction rack 12, the centrifugal device 30, the film tearing device 71, the film sealing device 72, and the temperature control device 40 are distributed on the first side of the track in the width direction, and the storage mechanism 11 and the plurality of extraction devices 50 are arranged on the second side of the track in the width direction, and the first side is opposite to the second side. The transfer device 60 automatically transfers the blood sample on the interaction rack 12 to the film tearing device 71 for film tearing, and then transfers the sample after film tearing to the dispensing device 20. The transfer device 60 can automatically transfer the reagent plate and consumables in the storage mechanism 11 to the dispensing device 20. In the moving direction of the mechanical arm along the track, the interaction rack 12, the centrifugal device 30, the film tearing device 71, and the film sealing device 72 are arranged in a row, and the storage mechanism 11 and the plurality of extraction devices 50 are arranged in a row adjacent to each other.

[0054] In some embodiments, please refer to Figures 1-3 The sample nucleic acid extraction device further comprises a base 80. The interaction rack 12, the centrifugal device 30, the extraction device 50, the track, the temperature control device 40, the film tearing device 71, and the film sealing device 72 are all installed on the top end of the base 80.

[0055] In some embodiments, please refer to Figure 3The film tearing device 71 and the film sealing device 72 are pullably installed on the base 80, can be moved close to the track and away from the track, and are moved away from the track when pulled out, facilitating manual addition of consumables required by the film tearing device 71 and the film sealing device 72. The base 80 can also be correspondingly provided with a buffer 73 and a proximity switch 74. The buffer 73 is used to buffer when the film tearing device 71 and the film sealing device 72 are pushed back, so as to avoid generating a large impact force. The proximity switch 74 is used to detect whether the film tearing device 71 and the film sealing device 72 are pushed back in place.

[0056] In some embodiments, referring to Figures 1-3 The base 80 includes a plurality of base tables 82, which are fixed by splicing assembly, facilitating assembly and subsequent use and maintenance. The storage mechanism 11 has a connecting block 111, and the storage mechanism 11 is connected and fixed with the adjacent base table 82 through the connecting block 111.

[0057] In some embodiments, referring to Figures 1-3 The base 80 has a hollow chamber with an opening facing the side surface of the base 80, and the top surface of the base 80 is provided with a through hole 81 communicating with the hollow chamber. The sample nucleic acid extraction device further includes a storage mechanism 91, which is detachably installed in the hollow chamber. The discarded plate and consumables can be thrown into the storage mechanism 91 through the through hole 81, so as to keep the surface of the base 80 clean and reduce the risk of sample contamination.

[0058] In some embodiments, referring to Figure 1 The base 80 is provided with a funnel-shaped material guide 92 communicating with the through hole 81 of the base 80, so as to facilitate accurate throwing of the discarded reagent plate and consumables into the storage mechanism 91, and effectively reduce the probability of cross contamination.

[0059] In some embodiments, referring to Figures 1-3 The sample nucleic acid extraction device further includes a code scanning gun 93, which is arranged on the interactive shelf 12 or arranged on the base 80 and close to the interactive shelf 12, so as to perform code scanning operation at the beginning of the automatic process, so as to reduce the error rate. The code scanning gun 93 is also arranged at the centrifugal device 30, so as to perform code scanning operation before centrifugal operation.

[0060] In some embodiments, referring to Figures 1-3, the sample nucleic acid extraction device further comprises a capping piece 94, the storage device 10 comprises a first support 14, the first support 14 is provided with a first positioning part matched with the capping piece 94, the capping piece 94 is placed on the first support 14 through the first positioning part, the first support 14 is located within the transfer range of the transfer device 60, and the capping piece 94 can be transferred by the transfer device 60 to the temperature control device 40, so that the capping piece 94 can be placed on the film-coated sample plate when the temperature control device 40 controls the temperature of the centrifuged sample plate. After the sample plate is coated, the bath treatment can effectively prevent liquid evaporation, by additionally placing the capping piece 94 on the sample plate, the capping piece 94 applies a certain pressure to the sample plate, which further effectively prevents liquid evaporation, and the capping piece 94 can also conduct heat and reduce the heat dissipation rate, which can effectively improve the bath effect of the sample.

[0061] In some embodiments, please refer to Figure 1 and Figure 3 , the sample nucleic acid extraction device further comprises a reagent tool for centrifugal balancing, the storage device 10 further comprises a second support 15, the second support 15 is provided with a second positioning part matched with the reagent tool, the capping piece 94 is placed on the second support 15 through the second positioning part, the second support 15 is located within the transfer range of the transfer device 60, and the reagent tool can be transferred by the transfer device 60 between the centrifugal device 30 and the second support 15, the reagent tool can be placed in the centrifugal device 30 together with the blood sample for centrifugation, and also plays a role in balancing.

[0062] The first positioning part and the second positioning part can refer to the structure of the first storage part, or can be any other feasible scheme.

[0063] In some embodiments, please refer to Figure 1 , the base 80 is further provided with a display, a keyboard, a mouse and a three-color lamp for human-computer interaction.

[0064] Next, taking the action flow of nucleic acid extraction of a blood sample as an example, the use method of the sample nucleic acid extraction device is described and introduced:

[0065] Before starting the automatic process, the reagent plate and consumables are manually filled into the storage mechanism 11, and then the sample plate to be processed is manually loaded and placed in the corresponding position of the interactive rack 12, and the capping piece 94 and the reagent tool are placed in the corresponding position. Then, the device is initialized, and the automatic process is started. The automatic process is as follows:

[0066] a) The transfer device 60 transports the sample plate to the film tearing device 71 to tear the film, and then transports the sample plate after tearing the film to the liquid separation device 20; the transfer device 60 transports the 96-hole suction head box from the storage mechanism 11 to the liquid separation device 20;

[0067] b) The transport device 60 transports the 96-well EB plate, standard plate, reagent conversion plate from the storage mechanism 11 to the film tearing device 71, and after the film tearing is completed, it is transferred to the dispensing device 20, and the dispensing device 20 performs the action of dispensing reagents into the sample;

[0068] c) After the pipetting is completed, the transport device 60 transfers the sample plate to the film sealing device 72, and after the film sealing is completed, it transports the sample plate to the centrifugal device 30 for centrifugation, and after the centrifugation is completed, it transfers the sample plate to the temperature control device 40, and the mechanical hand transfers the capping piece 94 to the temperature control device 40 and places the capping plate above the sample plate, and waits for the sample plate to react;

[0069] d) After the sample plate reacts for a preset time, the transport device 60 removes the capping piece 94 to the first support 14, and then grabs the sample plate from the temperature control device 40 to the dispensing device 20;

[0070] e) The transport device 60 transports the purified magnetic bead plate from the storage mechanism 11 to the film tearing device 71, and after the film tearing is completed, it is transferred to the dispensing device 20, and waits for the dispensing device 20 to perform the dispensing action;

[0071] f) After the pipetting is completed, the transport device 60 transfers the sample plate to the film sealing device 72, and after the film sealing is completed, it transports the sample plate to the extraction device 50; and then grabs the waste reagent conversion plate and the suction head box to the storage mechanism 91;

[0072] g) The transport device 60 sequentially grabs the washing plate, desulfurization plate, dissolution plate, and magnetic sleeve plate from the storage mechanism 11 to the extraction device 50, and the extraction device 50 performs the corresponding nucleic acid extraction processing operation;

[0073] h) After the nucleic acid extraction is completed, the mechanical hand takes and places the sample plate to the film sealing device 72, and after the film sealing is completed, it is transferred to the centrifugal device 30, and then sequentially grabs the waste washing plate, desulfurization plate, and magnetic sleeve plate to the storage mechanism 91;

[0074] i) The mechanical hand grabs and places the suction head box from the storage mechanism 11 to the dispensing device 20, and the mechanical hand sequentially takes and places the sample plate from the centrifugal device 30 to the film tearing device 71 and the dispensing device 20. The mechanical hand takes and places the real-time fluorescent quantitative PCR premix plate from the storage mechanism 11 to the film tearing device 71, and after the film tearing is completed, the mechanical hand moves the real-time fluorescent quantitative PCR premix plate to the dispensing device 20, and the dispensing device 20 performs the action of dispensing real-time fluorescent quantitative PCR premix into the sample;

[0075] j) After the pipetting is completed, the transport device 60 transfers the sample plate to the film sealing device 72 for film sealing, and after the film sealing is completed, the mechanical hand transports the sample plate to the centrifugal device 30 for centrifugation, and after the above actions are completed, the mechanical hand places the sample plate to the unloading area of the interaction rack 12;

[0076] k) The above actions are sequentially cycled.

[0077] The sample nucleic acid extraction device provided by the embodiment can be used for 96-well plate sample extraction and purification, and automatic pipetting can effectively improve pipetting quality, eliminate the risk of human reagent addition or omission, and reduce work intensity. Automatic code scanning and checking are performed, and manual sample plate transfer is not required, thereby reducing work intensity. The device has the functions of carrying, enzyme reaction, film sealing, film tearing, centrifugation, pipetting, temporary storage, and extraction, is suitable for medium and high sample extraction development, and can be used for sample extraction of intestinal cancer, Meta, HPV, WGS, and the like, and can complete the full-automatic nucleic acid extraction and purification process of medium and high throughput and unattended operation.

[0078] The above application of specific examples is used to describe the utility model, which is only used to help understand the utility model and does not limit the utility model. For the skilled person in the technical field to which the utility model belongs, according to the idea of the utility model, a number of simple deductions, deformations or substitutions can be made.

Claims

1. A sample nucleic acid extraction apparatus, characterized by, The sample nucleic acid extraction equipment comprises: a sample placing device, which comprises a first sample placing part and a second sample placing part, the first sample placing part is used for placing samples to be treated, and the second sample placing part is used for storing consumables and required reagent materials; a dispensing device, which is used for automatically dispensing reagents into the samples to be treated; a centrifugal device, which is used for centrifuging the mixture of the samples and the reagents; a temperature control device, which is used for controlling the temperature of the sample plate after centrifugation; an extraction device, which is used for extracting nucleic acid from the samples after temperature control; and a transfer device, the first sample placing part, the dispensing device, the centrifugal device, the temperature control device and the extraction device are located within the transfer range of the transfer device, and the transfer device is used for automatically transferring the samples, the consumables and the reagent materials between at least the first sample placing part, the dispensing device, the centrifugal device, the temperature control device and the extraction device.

2. The sample nucleic acid extraction apparatus according to claim 1, wherein The transfer device comprises a track, a mechanical hand and a driving mechanism, the mechanical hand is slidingly installed on the track, the driving mechanism is drivingly connected to the mechanical hand, the driving mechanism drives the mechanical hand to move along the track, and the first sample placing part, the second sample placing part, the dispensing device, the centrifugal device, the temperature control device and the extraction device are distributed on at least one side of the track.

3. The sample nucleic acid extraction apparatus according to claim 2, wherein The track is a straight track, and the first sample placing part, the second sample placing part, the dispensing device, the centrifugal device, the temperature control device and the extraction device are distributed on opposite sides of the track.

4. The sample nucleic acid extraction apparatus according to claim 3, wherein The number of the extraction devices is multiple, and multiple extraction devices are arranged on the same side of the track and sequentially arranged along the length direction of the track.

5. The sample nucleic acid extraction apparatus according to claim 3, wherein The sample placing device comprises a storage mechanism and an interactive frame, the storage mechanism has a containing cavity, and the containing cavity constitutes the second sample placing part; the first sample placing part is arranged on the interactive frame, and at least the interactive frame is located within the transfer range of the transfer device.

6. The sample nucleic acid extraction apparatus according to claim 5, wherein The sample nucleic acid extraction equipment further comprises a film tearing device, the film tearing device is used for tearing the film of the hole plate with the film, and the film tearing device is located within the transfer range of the transfer device. The sample nucleic acid extraction equipment further comprises a film sealing device, the film sealing device is used for automatically sealing the holes of the hole plate containing the samples, and the film sealing device is located within the transfer range of the transfer device.

7. The sample nucleic acid extraction apparatus according to claim 6, wherein The storage mechanism and the interactive frame are located within the transfer range of the transfer device. The sample nucleic acid extraction equipment comprises the film tearing device and the film sealing device; the interactive frame, the centrifugal device, the film tearing device, the film sealing device and the temperature control device are distributed on a first side in the width direction of the track, the storage mechanism and multiple extraction devices are arranged on a second side in the width direction of the track, the first side is opposite to the second side, and in the moving direction of the mechanical hand along the track, the interactive frame, the centrifugal device, the film tearing device and the film sealing device are arranged in a column, and the storage mechanism and multiple extraction devices are arranged in a column and adjacent to each other.

8. The sample nucleic acid extraction apparatus according to claim 7, wherein The sample nucleic acid extraction device further comprises a base, and the interaction rack, the centrifugal device, the extraction device, the track, the temperature control device, the film tearing device and the film sealing device are all installed on the top end of the base.

9. The sample nucleic acid extraction apparatus according to claim 8, wherein The base has a hollow chamber with an opening facing the side of the base, and the top surface of the base is provided with a through hole in communication with the hollow chamber. The sample nucleic acid extraction device further comprises a storage mechanism which is detachably installed in the hollow chamber. The waste plate and consumables can be put into the storage mechanism through the through hole.

10. The sample nucleic acid extraction apparatus of any one of claims 1-9, wherein, The sample nucleic acid extraction device further comprises a capping piece, and the storage device comprises a first support provided with a first positioning part matched with the capping piece. The capping piece is placed on the first support through the first positioning part. The first support is within the transfer range of the transfer device, and the capping piece can be transferred from the transfer device to the temperature control device, so that the capping piece can be placed on the film-coated sample plate when the temperature control device controls the temperature of the centrifuged sample plate.

11. The sample nucleic acid extraction apparatus of claim 10, wherein The sample nucleic acid extraction device further comprises a reagent tool for centrifugal balancing, and the storage device further comprises a second support provided with a second positioning part matched with the reagent tool. The capping piece is placed on the second support through the second positioning part. The second support is within the transfer range of the transfer device, and the reagent tool can be transferred between the centrifugal device and the second support by the transfer device.

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