Cell culture tube
By designing cell culture tubes that are wider at the top and narrower at the bottom, and combining culture and centrifugation functions, the problems of culture medium transfer contamination and inaccurate aspiration were solved, thereby increasing the cell growth area and the accuracy of experiments, and simplifying the operation process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-28
- Publication Date
- 2026-03-27
AI Technical Summary
In current cell culture processes, the culture medium is easily contaminated by external factors during transfer, the supernatant is not accurately removed after centrifugation, the cell growth area is insufficient, and high sealing requirements are required.
Design a cell culture tube that is wider at the top and narrower at the bottom, combining culture and centrifugation functions. It has an upper culture tube section and a lower centrifugation tube section, with flat and stepped surfaces on the inner wall, and is equipped with baffles and a cover to ensure the culture medium is sealed and the liquid is accurately aspirated.
It avoids contamination during culture medium transfer, increases cell growth area, ensures the accuracy and reliability of experiments, and simplifies the operation process.
Smart Images
Figure CN224047410U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of cell culture equipment, and in particular to a cell culture tube. Background Technology
[0002] In biological and medical research, cell culture tubes are commonly used equipment for in vitro cell culture, used to study the biological properties and molecular biological mechanisms of cells.
[0003] Conventional cell culture involves two steps: First, cells are cultured in a culture flask. Second, after culturing, the culture medium is transferred to centrifuge tubes, which are then centrifuged. After centrifugation, the supernatant is aspirated, and the precipitate is retained for later use. However, current culture media are exposed to the environment during transfer, making them susceptible to contamination. Furthermore, the aspiration of the supernatant after centrifugation can easily result in the accidental aspiration of the precipitate. While some methods involve directly placing cells into centrifuge tubes, these are currently circular. When placed horizontally or vertically, cells tend to accumulate at the lowest point, reducing the growth surface area and hindering cell division and growth. Additionally, if the centrifuge tubes are laid horizontally, the culture medium can easily leak out, contaminating the incubator and requiring a high degree of airtightness. Utility Model Content
[0004] The purpose of this invention is to provide a cell culture tube that can solve the problem of easy contamination during the above-mentioned culture process. It eliminates the need to transfer culture medium, has the advantages of saving the transfer step, making it more convenient to use, avoiding external environmental interference, and ensuring culture quality.
[0005] To achieve the above objectives, the solution of this utility model is:
[0006] A cell culture tube, comprising a tube body and a cap;
[0007] The tube is a hollow tube that is wider at the top and narrower at the bottom, including a culture tube section that is wider at the top and a centrifuge tube section that is narrower at the bottom.
[0008] The culture tube has a culture cavity, and at least one inner wall surface of the culture cavity is a plane; the upper end of the culture tube is a tube opening that communicates with the culture cavity, and the cover is detachably sealed at the tube opening;
[0009] The lower end of the culture tube has a lower opening that connects to the culture cavity, and this lower opening is connected to the centrifuge tube opening at the upper end of the centrifuge tube. The edge of the lower opening is smoothly connected to the edge of the centrifuge tube opening, and a stepped surface is formed between the lower end of the culture tube and the upper end of the centrifuge tube.
[0010] Further, the culture tube part comprises a left half-circular tube and a right half-rectangular tube connected to each other; the centrifugal tube part is in the shape of a top-opened cylindrical test tube; the centrifugal tube part is connected to a left bottom plate arranged below the left half-circular tube, the inner diameter of the centrifugal tube part is smaller than that of the half-circular tube, and a right bottom plate is arranged at the bottom of the right half-rectangular tube to form the stepped surface.
[0011] Further, the top of the culture tube part further comprises a hollow cylindrical connecting section and a hollow transition connecting section, the bottom of the cylindrical connecting section is connected to the top of the left half-circular tube and the right half-rectangular tube through the transition connecting section, the outer periphery of the cylindrical connecting section is formed with external threads, and the cover body is used for being screwed to the outer periphery of the cylindrical connecting section.
[0012] Further, the inner wall surface of the right bottom plate of the right half-rectangular tube is in the shape of an arc surface.
[0013] Further, the opening of the tube body is arranged close to one side of the plane and is provided with a baffle extending towards the other side, and the baffle does not completely block the opening of the tube body.
[0014] Further, the extension length of the baffle is one fourth to one half of the width of the opening of the tube body.
[0015] Further, the baffle is integrally formed with the tube body.
[0016] Further, the height of the upper culture tube part is greater than that of the lower centrifugal tube part.
[0017] Further, the bottom of the centrifugal tube part is in the shape of a circle or a cone.
[0018] After the above technical scheme is adopted, the culture tube part and the centrifugal tube part can be combined in the same tube body, the culture liquid does not need to be transferred in the whole test process, the culture liquid is always enclosed in the tube body, external pollution can be avoided, and the test accuracy and reliability are ensured. In addition, the plane of the culture cavity of the culture tube part can increase the culture area, when the tube body is horizontally placed for culture, the cells can be laid on the plane, and the growth of the cells is facilitated. After the tube body is directly taken to the centrifugal machine for centrifugal separation, the stepped surface can be formed between the upper culture tube part and the lower centrifugal tube part due to the narrow centrifugal tube part, therefore, when the supernatant is sucked by a pipette, the pipette tip can abut against the stepped surface to suck the supernatant, the precipitate can be avoided, and the test result is accurate and lossless.
[0019] Further, the baffle can prevent leakage and avoid pollution of the incubator even if the cover body is not sealed well. BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1 It is a perspective view of the embodiment of the utility model.
[0021] Figure 2 The front view of the utility model embodiment;
[0022] Figure 3 The exploded view of the utility model embodiment;
[0023] Figure 4 The cross-sectional view of the utility model embodiment;
[0024] Figure 5 The schematic view of the horizontal placement culture of the utility model embodiment;
[0025] Figure 6 The schematic view after centrifugation of the utility model embodiment;
[0026] Figure 7 The schematic view of the utility model embodiment placed in the centrifuge.
[0027] Explanation of reference numerals: tube body 1, culture tube part 11, culture inner cavity 111, plane 112, tube body opening 113, lower end opening 114, left side semicircular tube 115, right side semicircular tube 116, left bottom plate 117, right bottom plate 118, placement plane 119, centrifugal tube part 12, centrifugal tube opening 121, step surface 13, cylindrical connecting section 14, external thread 141, transition connecting section 15, baffle 16, cover 2, culture solution 3, precipitate 4, supernatant 5, centrifuge 6, centrifugal groove 61. DETAILED DESCRIPTION
[0028] To make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.
[0029] As shown in Figures 1 to 6 The cell culture tube of the embodiment comprises a tube body 1 and a cover 2.
[0030] The tube body 1 is a hollow tube body 1 with the upper part wide and the lower part narrow, comprising an upper culture tube part 11 and a lower centrifugal tube part 12;
[0031] The culture tube part 11 has a culture inner cavity 111, and at least one inner wall surface of the culture inner cavity 111 is a plane 112; the upper end of the culture tube part 11 is a tube body opening 113 communicating with the culture inner cavity 111, and the cover 2 is detachably and sealingly arranged at the tube body opening 113;
[0032] The lower end of the culture tube part 11 has a lower end opening 114 communicating with the culture inner cavity 111, and the lower end opening 114 communicates with the centrifugal tube opening 121 at the upper end of the centrifugal tube part 12, and the edge of the lower end opening 114 is connected to the edge of the centrifugal tube opening 121 in a smooth transition, and a step surface 13 is formed between the lower end of the culture tube part 11 and the upper end of the centrifugal tube part 12.
[0033] Therefore, when using the cell culture tube, first, the cover 2 is opened, and the culture medium is added into the culture inner cavity 111 from the tube body opening 113, and then the cells to be cultured are added, for example, 4 ml of culture medium is added, and 0.5 ml of blood is added; then the two are mixed to form a culture solution 3, and the cover 2 is closed to seal the tube body opening 113, and then the tube body 1 is placed horizontally with the plane 112 facing up (see Figure 5 ) in the incubator, the plane 112 of the culture inner cavity 111 can increase the culture area, and when the tube body 1 is placed horizontally, the cells in the culture solution 3 can be spread on the plane 112, which is beneficial to the growth of the cells.
[0034] After a period of culture, for example, after three days, the tube body 1 and the cover 2 are directly taken to the centrifuge 6 for centrifugation, so that the culture solution is centrifuged and layered, and during the centrifugation process, the tube body opening 113 faces up and the bottom faces down, so that the centrifugal precipitate 4 can be precipitated and collected in the centrifugal tube part 12 at the bottom of the tube body 1 (see Figure 6 ), after the centrifugation is completed, the cover 2 can be opened, and the supernatant 5 is removed by a pipette to retain the precipitate 4, and because the centrifugal tube part 12 is narrower and can form a step surface 13 with the bottom of the upper culture tube part 11, when the supernatant 5 is removed by the pipette, the tip of the pipette can abut against the step surface 13 to suck the supernatant 5, which can avoid sucking the precipitate 4, and ensures the accuracy of the test results without loss.
[0035] As shown in Figure 7 , the low-speed centrifuge 6 with model KDC-2046 (Keda Innovation Co., Ltd.) can be used for centrifugation operation of the cell culture tube, and only the outer shape size of the cell culture tube needs to be inserted into the centrifugal groove 61.
[0036] In this embodiment, the culture and centrifugation are combined in the same tube body 1, and during the whole test process, the culture solution does not need to be transferred, and the culture solution is always sealed in the tube body 1, which can avoid external pollution and ensure the accuracy and reliability of the test.
[0037] In this embodiment, as shown in Figure 3 and Figure 4The culture tube part 11 can include a left half-circular tube 115 and a right half-rectangular tube 116 connected to each other; the centrifugal tube part 12 is in the shape of a top-opened cylindrical test tube; the centrifugal tube part 12 is connected to a left bottom plate 117 arranged below the left half-circular tube 115, the inner diameter of the centrifugal tube part 12 is smaller than that of the half-circular tube, and the right half-rectangular tube 116 is provided with a right bottom plate 118 at the bottom to form the stepped surface 13.
[0038] The inner wall of the right half-rectangular tube 116 can form the above-mentioned flat surface 112, and the outer wall can form a placement flat surface 119, which can be used to horizontally place the tube body 1 with the right side downward in a culture box, so as to keep the tube body 1 stable and prevent it from rolling, and the tube body 1 does not need to be fixed by a test tube clamp, which is more convenient to use.
[0039] In the embodiment, the culture tube part 11 is further provided with a hollow cylindrical connecting section 14 and a hollow transition connecting section 15 at the top, the bottom of the cylindrical connecting section 14 is connected to the top of the left half-circular tube 115 and the right half-rectangular tube 116 through the transition connecting section 15 to form an integrated whole; the outer periphery of the cylindrical connecting section 14 is provided with external threads 141; and the cover body 2 is used to be screwed and sealed to the outer periphery of the cylindrical connecting section 14. That is, the cover body 2 is provided with internal threads, so that the cover body 2 can be conveniently screwed and locked or unscrewed and removed, which is more convenient to use.
[0040] The tube body 1 of the embodiment can be made of plastic material. The whole tube body 1 can be integrally formed by injection molding, so that the left half-circular tube 115 and the right half-rectangular tube 116 and other structures can be conveniently produced.
[0041] As Figure 4 , the inner wall surface of the right bottom plate 118 of the right half-rectangular tube 116 can be in the shape of an arc, that is, the stepped surface 13 can be arc-shaped, so that the cells can conveniently slide and precipitate in the centrifugal tube part 12 at the bottom during centrifugation.
[0042] Referring back to Figure 3 , the tube body opening 113 can be provided with a baffle 16 extending towards the other side on the side close to the flat surface 112, and the baffle 16 does not completely block the tube body opening 113. In the embodiment, the baffle 16 extends from the right side to the left side of the tube body opening 113. Taking the circular opening as an example, the baffle 16 can be in the shape of a semicircle. The baffle 16 can be arranged to shield the culture solution when the tube body 1 is horizontally placed for culture, and the baffle 16 also has a shielding effect during the liquid extraction process after centrifugation, which can prevent liquid from spilling out.
[0043] Specifically, the baffle 16 can be integrally formed with the tube body 1. The extension length of the baffle 16 can be one fourth to one half of the width of the tube body opening 113. In the embodiment, the width of the tube body opening 113, that is, one half of its diameter, is taken as an example.
[0044] In the embodiment, the upper and lower height of the upper culture tube part 11 is greater than the upper and lower height of the lower centrifugal tube part 12. The bottom of the centrifugal tube part 12 can be circular or conical, and both can perform centrifugal operation. The embodiment takes the circular as an example.
[0045] The above is only the preferred embodiment of the present application, and the protection scope of the present application is not limited to the above-mentioned embodiments. Any technical solution falling within the concept of the present application belongs to the protection scope of the present application. It should be noted that equivalent changes and modifications without departing from the principles of the present application are still within the protection scope of the present application.
[0046] In the description of the embodiments of the present application, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly used when the product of the present application is placed, or the orientation or positional relationship commonly understood by those skilled in the art, and is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In the description of the present application, the meaning of "a plurality of" and "several" is two or more, unless otherwise specifically limited.
Claims
1. A cell culture tube, characterized in that: it comprises a tube body and a cover; the tube body is a hollow tube body with a wide upper part and a narrow lower part, comprising an upper wide culture tube part and a lower narrow centrifugal tube part; the culture tube part has a culture inner cavity, at least one side inner wall surface of which is a plane; the upper end of the culture tube part is a tube body opening communicating with the culture inner cavity, and the cover is detachably and sealingly arranged at the tube body opening; the lower end of the culture tube part has a lower end opening communicating with the culture inner cavity, and the lower end opening is connected to the centrifugal tube opening at the upper end of the centrifugal tube part, and the edge of the lower end opening and the edge of the centrifugal tube opening are connected in a smooth transition, and a step surface is formed between the lower end of the culture tube part and the upper end of the centrifugal tube part.
2. The cell culture tube of claim 1, wherein: the culture tube part comprises a left half-circular tube and a right half-rectangular tube connected in series; the centrifugal tube part is in the shape of an open-top cylindrical test tube; the centrifugal tube part is connected to a left bottom plate arranged below the left half-circular tube, and the inner diameter of the centrifugal tube part is smaller than that of the half-circular tube; and a right bottom plate is arranged at the bottom of the right half-rectangular tube to form the step surface.
3. The cell culture tube of claim 2, wherein: the top of the culture tube part further has a hollow cylindrical connecting section and a hollow transition connecting section; the bottom of the cylindrical connecting section is connected to the top of the left half-circular tube and the right half-rectangular tube through the transition connecting section; the outer periphery of the cylindrical connecting section is formed with external threads; and the cover is used for screwing and sealing to the outer periphery of the cylindrical connecting section.
4. The cell culture tube of claim 2, wherein: the inner wall surface of the right bottom plate of the right half-rectangular tube is in the shape of an arc surface.
5. The cell culture tube of claim 1, wherein: the side of the tube body opening close to the plane is provided with a baffle extending towards the other side, and the baffle does not completely block the tube body opening.
6. The cell culture tube of claim 5, wherein: the extension length of the baffle is one fourth to one half of the width of the tube body opening.
7. The cell culture tube of claim 5, wherein: the baffle is integrally formed with the tube body.
8. The cell culture tube of claim 1, wherein: the height of the upper culture tube part is greater than that of the lower centrifugal tube part.
9. The cell culture tube of claim 2, wherein: the bottom of the centrifugal tube part is in the shape of a circle or a cone.