Novel stem cell resuscitation culture device

Abstract

The utility model discloses a novel stem cell resuscitation culture device, which relates to the technical field of stem cell resuscitation and comprises a constant-temperature water tank, a sealing plate is arranged at the top of the constant-temperature water tank, fixing plates are fixedly connected to two ends of the bottom of the sealing plate, and a connecting shaft is rotatably connected between the two fixing plates. A plurality of fixing frames are fixedly connected to the bottom side of the connecting shaft, fixing assemblies are arranged at the bottom ends of the fixing frames, and cryopreservation pipes are installed at the bottoms of the fixing frames through the fixing assemblies. According to the cryopreservation tube fixing device, the cryopreservation tube can be fixed through the arranged fixing assembly, the movable disc is driven to rotate by pushing the handle, the three movable rods can be driven to move outwards, the spring is stressed to be compressed, then the cryopreservation tube is inserted into the inner ring of the movable disc, at the moment, the handle is loosened, and the cryopreservation tube is clamped and fixed through the acting force of the spring; and by arranging the multiple fixing frames and the fixing assemblies, the multiple cryopreservation tubes can be fixed at a time, and the working efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to stem cell resuscitation technical field, concretely is a novel stem cell resuscitation culture device. BACKGROUND

[0002] Stem cells are a kind of cells with self-renewal ability and multi-directional differentiation potential, can differentiate into multiple different types of cells under certain conditions, have important biological significance and extensive application prospect, stem cells can continuously produce the same cells as itself through cell division, maintain the stability of its quantity, stem cells can be used for treating a variety of diseases and drug research and development, stem cell research has made remarkable progress in the past few decades, but still faces some challenges.

[0003] Stem cell resuscitation is the process of restoring frozen stem cells to active state, stem cells are placed in low-temperature environment during freezing process, so that the metabolic activity of cells almost stops, the existing thawing resuscitation mode is usually to put the frozen tube into constant-temperature water bath, and gently shake it, so that it melts as soon as possible, in the prior art, the process is mostly manually operated by personnel, the personnel need to hold the frozen tube in warm water and shake it constantly, which not only increases the labor intensity of personnel, but also the number of frozen tubes taken by personnel at a time is limited, resulting in low thawing resuscitation efficiency, therefore, a novel stem cell resuscitation culture device is needed to solve the existing problems. CONTENT OF THE UTILITY MODEL

[0004] TECHNICAL PROBLEM SOLVED

[0005] The utility model aims at making up for the deficiency of prior art, providing a novel stem cell resuscitation culture device, which can fix multiple frozen tubes at a time, and automatically replace personnel to shake the frozen tube in constant-temperature water bath, to achieve the effect of stem cell resuscitation, to facilitate the operation of users.

[0006] TECHNICAL SCHEME

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a novel stem cell resuscitation culture device, comprising a constant-temperature water tank, the top of the constant-temperature water tank is provided with a sealing plate, the bottom of the sealing plate is fixedly connected with a fixed plate at both ends, a connecting shaft is rotatably connected between the two fixed plates, a plurality of fixed frames are fixedly connected to the bottom side of the connecting shaft, the bottom end of the fixed frame is provided with a fixed assembly, the bottom of the fixed frame is installed with a frozen tube through the fixed assembly, one side of the outer side of the fixed plate is fixedly connected with a driving motor, the output shaft of the driving motor is fixedly connected with one end of the connecting shaft, the rear side of the constant-temperature water tank is provided with a lifting assembly, the output end of the lifting assembly is fixedly connected with the top end of the sealing plate, the inner wall of one side of the constant-temperature water tank is fixedly connected with a temperature control electric heating rod, the outer side of the constant-temperature water tank is fixedly connected with a controller.

[0008] The utility model further sets up, the fixed component includes fixed disc, movable disc, movable rod and locating wheel, the top fixed connection of fixed disc is in the bottom of fixed frame, the outside rotation is connected in the inner wall of fixed disc of movable disc, the bottom respectively of movable rod and fixed disc and movable disc rotation is connected, the bottom fixed connection of locating wheel is in the top of movable rod.

[0009] The utility model further sets up, the fixed component still includes recess, connecting plate and spring, the recess sets up in the outside of constant temperature water tank, one end fixed connection of connecting plate is in the outside of movable disc, and connecting plate is located in the inside of recess, one end fixed connection of spring is in the inner wall of recess, the other end fixed connection of spring is in the outside of connecting plate.

[0010] The utility model further sets up, the fixed component still includes handle, one end fixed connection of handle is in the outside of movable disc, handle is located in the inside of recess.

[0011] The utility model further sets up, the lifting assembly includes vertical board, rotating rod and connecting rod no.

[0012] The utility model further sets up, the lifting assembly still includes limit stop, one side fixed connection of limit stop is in the outside of vertical board, the limit stop is covered in the surface of connecting rod no.

[0013] The utility model further sets up, the lifting assembly still includes connecting rod no.

[0014] Beneficial effects:

[0015] One, the utility model discloses a fixed component can freeze the storage pipe and fix, through the handle, drive movable disc rotation, can drive three movable rods and move outward, and make spring force compression, then insert the freeze storage pipe into the inner ring of movable disc, loosen the handle at this moment, through the force of spring freeze the storage pipe and hold fixed, through setting up a plurality of fixed frame and fixed component, can fix a plurality of freeze storage pipes at a time, improve work efficiency.

[0016] Second, the utility model discloses through the lifting assembly set, can freeze the storage pipe fixed at the bottom side of the sealing plate into the constant temperature water tank, through the drive motor drives the connecting shaft reciprocating rotation, can drive freeze the storage pipe and reciprocate, make stem cell fast recovery, replace personnel manual operation, reduce personnel labor intensity.

[0017] Other advantages, objects and features of the present utility model will be in part elucidated in the following specification, and in part, will be obvious to those skilled in the art based on the study of the following, or can be taught from the practice of the present utility model. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is the device whole schematic view of the utility model;

[0019] Figure 2 It is the constant temperature water tank internal structure schematic view of the utility model;

[0020] Figure 3 It is the sealing plate bottom structure schematic view of the utility model;

[0021] Figure 4 It is the fixed assembly structure schematic view of the utility model;

[0022] Figure 5 It is the fixed assembly structure schematic view of the utility model;

[0023] Figure 6 It is the lifting assembly structure schematic view of the utility model.

[0024] In the drawing: 1, constant temperature water tank;2, sealing plate;3, fixed plate;4, connecting shaft;5, fixed frame;6, fixed assembly;601, fixed disc;602, movable disc;603, movable rod;604, positioning wheel;605, recess;606, connecting plate;607, spring;608, handle;7, freeze tube;8, drive motor;9, lifting assembly;901, vertical plate;902, rotating rod;903, connecting rod one;904, connecting rod two;905, limiting plate;906, air cylinder;10, temperature control electric heating rod;11, controller. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present utility model will be described clearly and completely below with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present utility model.

[0026] AsFigures 1-6 The utility model provides a technical scheme: a novel stem cell recovery culture device, including constant temperature water tank 1, the top of constant temperature water tank 1 is provided with sealing plate 2, the setting can be sealed to the top of constant temperature water tank 1 when shaking freeze storage tube 7, avoid the water overflow in constant temperature water tank 1, the bottom both ends of sealing plate 2 are fixedly connected with fixed plate 3, and the rotatable connection of two fixed plates 3 has connecting shaft 4, and the bottom side fixed connection of connecting shaft 4 has several fixed frames 5, and the bottom of fixed frame 5 is provided with fixed assembly 6, and freeze storage tube 7 is installed to fixed assembly 6 in the bottom of fixed frame 5, and the outside fixed connection of one of fixed plate 3 has drive motor 8, and the reciprocating rotation of connecting shaft 4 is driven by drive motor 8, can drive freeze storage tube 7 to reciprocate and shake, make stem cell fast recovery, and the output shaft of drive motor 8 is fixedly connected with one end of connecting shaft 4, and the rear side of constant temperature water tank 1 is provided with lifting assembly 9, and the output end of lifting assembly 9 is fixedly connected with the top of sealing plate 2, and the inside wall of one side of constant temperature water tank 1 is fixedly connected with temperature control electric heating stick 10, and the inside water is heated by temperature control electric heating stick 10, and after heating to 38-40 DEG C, stem cell fast recovery effect can be reached, and the outside of constant temperature water tank 1 is fixedly connected with controller 11, and controller 11 can show the water temperature in constant temperature water tank 1, and personnel is convenient to the water temperature control;

[0027] When using, lifting assembly 9 drives sealing plate 2 to move to the top of constant temperature water tank 1, then personnel takes out the stem cell freeze storage tube 7 in cold storage, and freeze storage tube 7 is fixed in the bottom of fixed frame 5 by fixed assembly 6, by setting multiple fixed frames 5 and fixed assembly 6, multiple freeze storage tubes 7 can be fixed at a time, when freeze storage tube 7 is fixed, freeze storage tube 7 is moved to the inside of constant temperature water tank 1 by lifting assembly 9 and recovers thawing.

[0028] As Figure 3 , Figure 4 , Figure 5 And Figure 6As shown, the fixing component 6 includes a fixed plate 601, a movable plate 602, a movable rod 603, a positioning wheel 604, a groove 605, a connecting plate 606, a spring 607, and a handle 608. The top of the fixed plate 601 is fixedly connected to the bottom of the fixing frame 5. The outer side of the movable plate 602 is rotatably connected to the inner wall of the fixed plate 601. The bottom of the movable rod 603 is rotatably connected to both the fixed plate 601 and the movable plate 602. The bottom of the positioning wheel 604 is fixedly connected to the top of the movable rod 603. The groove 605 is formed on the outer side of the constant temperature water tank 1. One end of the connecting plate 606 is fixedly connected to the outer side of the movable plate 602, and the connecting plate 606 is located inside the groove 605. One end of the spring 607 is fixedly connected to the inner wall of the groove 605, and the other end of the spring 607 is fixedly connected to the outer side of the connecting plate 606. One end of the handle 608 is fixedly connected to the outer side of the movable plate 602, and the handle 608 is located inside the groove 605.

[0029] The cryopreservation tube 7 is fixed to the bottom of the fixing frame 5 by the fixing component 6. By pushing the handle 608, the movable plate 602 is rotated, which can drive the three movable rods 603 to move outward and compress the spring 607. Then, the cryopreservation tube 7 is inserted into the inner ring of the movable plate 602. At this time, the handle 608 is released, and the cryopreservation tube 7 is clamped and fixed by the force of the spring 607. By setting multiple fixing frames 5 and fixing components 6, multiple cryopreservation tubes 7 can be fixed at one time, improving work efficiency.

[0030] like Figure 1 , Figure 2 and Figure 6 As shown, the lifting assembly 9 includes a vertical plate 901, a rotating rod 902, a first connecting rod 903, a second connecting rod 904, a limiting plate 905, and a cylinder 906. The bottom end of the vertical plate 901 is fixedly connected to the top end of the constant temperature water tank 1. The two sides of the rotating rod 902 are rotatably connected to the top end of the vertical plate 901. The top end of the first connecting rod 903 is movably connected to one end of the rotating rod 902 via a rotating shaft, and the bottom end of the first connecting rod 903 is fixedly connected to the top end of the sealing plate 2. The top end of the second connecting rod 904 is movably connected to one end of the rotating rod 902 via a rotating shaft. One side of the limiting plate 905 is fixedly connected to the outside of the vertical plate 901. The limiting plate 905 is sleeved on the surface of the first connecting rod 903, and the limiting plate 905 is slidably connected to the surface of the first connecting rod 903. The bottom end of the cylinder 906 is fixedly connected to the top end of the constant temperature water tank 1, and the output shaft of the cylinder 906 is fixedly connected to the bottom end of the second connecting rod 904.

[0031] Through the lifting assembly 9 arranged, the cryopreservation tube 7 fixed on the bottom side of the sealing plate 2 can be extended into the constant-temperature water tank 1, the connecting rod two 904 is driven to move up and down by starting the air cylinder 906, the rotating rod 902 is rotated on the vertical plate 901, the connecting rod one 903 is limited by the limiting plate 905, the connecting rod one 903 is driven to move up and down, and the sealing plate 2 is driven to move up and down.

[0032] Working principle: when in use, the constant-temperature water tank 1 is filled with water, and the water inside is heated to 38-40 DEG C by the temperature control electric heating rod 10, the connecting rod two 904 is driven to move downwards by starting the air cylinder 906, the rotating rod 902 is rotated on the vertical plate 901, the connecting rod one 903 is limited by the limiting plate 905, the connecting rod one 903 is driven to move upwards, and the sealing plate 2 is moved to the top of the constant-temperature water tank 1, then the staff takes out the cryopreservation tube 7 of the frozen stem cells, and the cryopreservation tube 7 is fixed at the bottom of the fixed frame 5 through the fixing assembly 6, a plurality of cryopreservation tubes 7 can be fixed at one time by arranging a plurality of fixed frames 5 and fixing assemblies 6, when the cryopreservation tube 7 is fixed, the connecting rod two 904 is driven to move upwards by starting the air cylinder 906, the sealing plate 2 is moved downwards and covers the top of the constant-temperature water tank 1, and the cryopreservation tube 7 is moved to the inside of the constant-temperature water tank 1, finally the connecting shaft 4 is driven to rotate reciprocatingly by the driving motor 8, and the cryopreservation tube 7 is driven to reciprocate, so that the stem cells are quickly recovered.

[0033] When the fixing assembly 6 fixes the cryopreservation tube 7, the staff drives the movable disc 602 to rotate by pushing the handle 608, the connecting plate 606 is moved and the spring 607 is compressed, the spring 607 is compressed under stress, the movable disc 602 is moved, the three movable rods 603 are moved outwards, then the cryopreservation tube 7 is inserted into the inner ring of the movable disc 602, the handle 608 is released, the spring 607 is reset under the action of force, the connecting plate 606 and the movable disc 602 are reset, and the three connecting rods movable rods 603 are moved towards the cryopreservation tube 7, so that the cryopreservation tube 7 is clamped and fixed.

[0034] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, not limit the present application, although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the spirit and scope of the technical solutions of the present application, which should be covered in the scope of claims of the present application.

Claims

1. A novel stem cell recovery culture device comprising a thermostatic water tank (1), characterized in that: The top of the constant-temperature water tank (1) is provided with a sealing plate (2), the bottom of the sealing plate (2) is fixedly connected with a fixed plate (3) at both ends, a connecting shaft (4) is rotatably connected between the two fixed plates (3), a plurality of fixed frames (5) are fixedly connected to the bottom side of the connecting shaft (4), a freezing tube (7) is installed on the bottom of the fixed frame (5) through a fixing assembly (6), the outer side of one of the fixed plates (3) is fixedly connected with a driving motor (8), the output shaft of the driving motor (8) is fixedly connected with one end of the connecting shaft (4), the rear side of the constant-temperature water tank (1) is provided with a lifting assembly (9), the output end of the lifting assembly (9) is fixedly connected with the top end of the sealing plate (2), the inner wall of one side of the constant-temperature water tank (1) is fixedly connected with a temperature control electric heating rod (10), and the outer side of the constant-temperature water tank (1) is fixedly connected with a controller (11).

2. A novel stem cell recovery culture device according to claim 1, wherein: The fixing assembly (6) comprises a fixed disc (601), a movable disc (602), a movable rod (603) and a positioning wheel (604), the top end of the fixed disc (601) is fixedly connected to the bottom end of the fixed frame (5), the outer side of the movable disc (602) is rotatably connected to the inner wall of the fixed disc (601), the bottom of the movable rod (603) is rotatably connected with the fixed disc (601) and the movable disc (602) respectively, and the bottom end of the positioning wheel (604) is fixedly connected to the top end of the movable rod (603).

3. A novel stem cell recovery culture device as claimed in claim 2, wherein: The fixing assembly (6) further comprises a groove (605), a connecting plate (606) and a spring (607), the groove (605) is formed in the outer side of the constant-temperature water tank (1), one end of the connecting plate (606) is fixedly connected to the outer side of the movable disc (602), and the connecting plate (606) is located in the inside of the groove (605), one end of the spring (607) is fixedly connected to the inner wall of the groove (605), and the other end of the spring (607) is fixedly connected to the outer side of the connecting plate (606).

4. The novel stem cell recovery culture device according to claim 3, wherein: The fixing assembly (6) further comprises a handle (608), one end of the handle (608) is fixedly connected to the outer side of the movable disc (602), and the handle (608) is located in the inside of the groove (605).

5. The novel stem cell recovery culture device according to claim 4, wherein: The lifting assembly (9) comprises a vertical plate (901), a rotating rod (902) and a connecting rod (903), the bottom end of the vertical plate (901) is fixedly connected to the top end of the constant-temperature water tank (1), the two sides of the rotating rod (902) are rotatably connected to the top end of the vertical plate (901), and the top end of the connecting rod (903) is movably connected to one end of the rotating rod (902) through a rotating shaft, and the bottom end of the connecting rod (903) is fixedly connected to the top end of the sealing plate (2).

6. A novel stem cell recovery culture device as claimed in claim 5, wherein: The lifting assembly (9) further comprises a limiting plate (905), one side of the limiting plate (905) is fixedly connected to the outer side of the vertical plate (901), the limiting plate (905) is sleeved on the surface of the connecting rod (903), and the limiting plate (905) is slidably connected with the surface of the connecting rod (903).

7. A novel stem cell recovery culture device as claimed in claim 6, wherein: The lifting assembly (9) further comprises a connecting rod II (904) and a cylinder (906), the top end of the connecting rod II (904) is movably connected to one end of the rotating rod (902) through a rotating shaft, the bottom end of the cylinder (906) is fixedly connected to the top end of the constant-temperature water tank (1), and the output shaft of the cylinder (906) is fixedly connected to the bottom end of the connecting rod II (904).

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