Agarose glue boiling device
By integrating stirring and heating into the agarose gel cooking device, and utilizing the cooperation of the drive unit and the circulation pump, the problem of uneven heating of agarose gel was solved, achieving uniform heating of the solution and improving the accuracy of electrophoresis results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2026-03-31
AI Technical Summary
In existing technologies, agarose gels are prone to localized overheating or uneven stirring during the heating process, which affects the gel quality and the accuracy of electrophoresis results.
An agarose gel cooking device was designed, which integrates stirring and heating into one unit. The stirring tube is driven to rotate by a drive component, and the solution is heated evenly by a circulating pump that continuously flows high-temperature hot oil, ensuring uniform heat transfer.
Uniform heating of the agarose solution was achieved, which improved gel quality and accuracy of electrophoresis results. The design of the seal and retainer prevented hot oil leakage.
Smart Images
Figure CN224066443U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the biological experiment equipment technical field, especially relate to an agarose gel boiling device. BACKGROUND
[0002] In biological experiments, agarose gel electrophoresis is a commonly used experimental technique for separating, identifying and purifying biological macromolecules such as DNA, RNA, etc. Before performing agarose gel electrophoresis, the agarose needs to be dissolved in a specific buffer and heated to form a uniform gel solution.
[0003] Currently, the agarose gel is usually boiled using ordinary heating equipment such as a microwave oven, electric stove, etc., and then manually stirred to promote the dissolution of the agarose. However, when using a microwave oven to heat, local overheating easily occurs, resulting in uneven heating of the agarose gel, affecting the quality of the gel and in turn affecting the accuracy of the electrophoresis results. When using an electric stove to heat, if the stirring is not timely or uniform, similar problems may also occur. SUMMARY
[0004] The utility model aims at the problems existing in the prior art, provides an agarose gel boiling device, which integrates stirring and heating in one, so that the stirring part gradually and uniformly heats the solution containing agarose during the stirring process until the agarose melts.
[0005] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme: an agarose gel boiling device, characterized in that it comprises a heating oil tank, a gel boiling barrel is arranged on the heating oil tank, the gel boiling barrel comprises a barrel body and a barrel cover sleeved on the top end of the barrel body, a first pipe member extending out of the heating oil tank is arranged vertically inside the heating oil tank, and a driving member is arranged to drive the first pipe member to rotate, a stirring pipe extending to the bottom of the barrel body is arranged inside the barrel body, the stirring pipe is connected with the first pipe member, a transition pipe is arranged at the center of the barrel cover and detachably connected with the stirring pipe, a circulating pump is arranged on the heating oil tank to draw hot oil inside the heating oil tank, a delivery pipe is arranged at the output end of the circulating pump, and a second pipe member is movably connected with the transition pipe at the other end of the delivery pipe.
[0006] Through the above technical means, the first pipe member is driven to rotate by the driving member, and the stirring pipe connected with the first pipe member rotates synchronously. During the rotation of the stirring pipe, the circulating pump is used to make high-temperature hot oil continuously flow in the stirring pipe, and the stirring pipe is heated by the high-temperature hot oil to become a heating member to heat the solution containing agarose in the barrel body. Since the stirring pipe is constantly rotating, the stirring pipe can quickly and uniformly transfer heat to the solution mixture in the barrel body, thereby achieving uniform heating and effectively ensuring the quality of the gel and improving the accuracy of the electrophoresis results.
[0007] Optionally, the heating oil tank is provided with a heating wire for heating the oil in the heating oil tank, and a controller is arranged on one side of the heating oil tank for controlling the switch and operation time of the heating wire, the driving member and the circulating pump.
[0008] Optionally, the bottom wall of the barrel body is provided with an embedded groove matched with the first pipe, the bottom end of the stirring pipe extends into the embedded groove, the stirring pipe is rotatably connected to the bottom wall of the barrel body through a sealing member, and the outer periphery of the bottom end of the stirring pipe and located in the embedded groove is provided with a clamping ring seat matched with the top end of the first pipe. The design of the embedded groove enables the stirring pipe to be more stably installed in the barrel body, and forms a close fit with the first pipe, ensuring that the hot oil can flow smoothly into the stirring pipe for heating. The design of the clamping ring seat enables the stirring pipe to be clamped with the first pipe, so that the first pipe can drive the stirring pipe to rotate when the first pipe rotates, and the sealing member ensures that the stirring pipe does not leak hot oil during rotation.
[0009] Optionally, the top wall of the heating oil tank is provided with a mounting box, and the first pipe and the driving member are arranged in the mounting box, the first pipe vertically penetrates the mounting box, and the first pipe is rotatably connected to the heating oil tank and the mounting box.
[0010] Optionally, the driving member includes a motor, and a driving gear connected with the first pipe is arranged on the output shaft of the motor.
[0011] Optionally, the top end of the first pipe is provided with a clamping groove matched with the clamping ring seat, the outer periphery of the first pipe is provided with a limiting ring rotatably connected to the inner bottom wall of the mounting box, and the surface of the first pipe is provided with a gear ring engaged with the driving gear.
[0012] Through the above technical solution, the design of the clamping groove enables the stirring pipe to be firmly clamped with the first pipe, and the stirring function is realized by the rotation of the driving gear. The limiting ring ensures the stability of the first pipe during rotation.
[0013] Optionally, the stirring pipe is a serpentine pipe, the top end and the bottom end of the stirring pipe are located on the axis of the barrel body, and the top end of the stirring pipe is provided with a sleeve seat movably connected with the transition pipe.
[0014] Through the above technical solution, the stirring pipe is designed as a serpentine pipe, which increases the stirring area and improves the stirring efficiency. The design of the sleeve seat enables the stirring pipe to be stably connected with the transition pipe and the first pipe.
[0015] Optionally, the second pipe comprises a first adapter pipe connected with the conveying pipe, the first adapter pipe is provided with an extension pipe at the other end, the extension pipe is provided with a second adapter pipe movably connected with the transition pipe, and the extension pipe is flexible and can be connected with or separated from the transition pipe.
[0016] Optionally, the transition pipe is a sleeve pipe with a T-shaped cross section, the thick section of the transition pipe is located at the top of the barrel cover, the bottom end of the second adapter pipe is movably connected with the thick section of the transition pipe, the thin section of the transition pipe passes through the barrel cover, and the bottom end of the thin section of the transition pipe is movably connected with the sleeve seat.
[0017] Compared with the prior art, the utility model has the advantages that: 1. the driving part drives the first pipe to rotate, and the stirring pipe connected with the first pipe rotates synchronously, and in the process of rotating the stirring pipe, the circulating pump keeps the high-temperature hot oil flowing in the stirring pipe, the high-temperature hot oil heats the stirring pipe to become a heating part to heat the solution with agarose in the barrel, the stirring pipe rotates continuously, so that the stirring pipe can quickly and uniformly transfer heat to the solution mixture in the barrel, thereby realizing uniform heating and effectively guaranteeing the gel quality and improving the accuracy of the electrophoresis result; 2. the stirring pipe is designed as a serpentine pipe, the stirring area is increased, and the stirring efficiency is improved; the sleeve seat and the clasp seat are designed to firmly connect the stirring pipe with the transition pipe and the first pipe; and the sealing part is arranged to prevent the hot oil from leaking in the process of rotating the stirring pipe; 3. the extension pipe is flexible and can be connected with or separated from the transition pipe. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 It is a cross-sectional view of the agarose gel boiling device of the utility model;
[0019] Figure 2 It is a front side view of the three-dimensional view of the heating oil tank of the utility model;
[0020] Figure 3 It is a rear side view of the three-dimensional view of the heating oil tank of the utility model;
[0021] Figure 4 It is a top view of the three-dimensional view of the barrel of the utility model;
[0022] Figure 5 It is a bottom view of the three-dimensional view of the barrel of the utility model;
[0023] Figure 6 It is a schematic view of the connection relationship among the second pipe, the transition pipe and the stirring pipe of the utility model;
[0024] Figure 7The utility model discloses a connection relation schematic drawing of stirring pipe, barrel body bottom wall, first pipe fitting and driving part.
[0025] Figure 8 The utility model discloses a first pipe fitting schematic drawing.
[0026] Figure 9 The utility model discloses a second pipe fitting schematic drawing.
[0027] In the figure: 1, heating oil tank;11, heating wire;12, controller;13, placing groove;2, cook glue barrel;21, barrel body;211, inlay groove;22, barrel cover;221, transition pipe;3, first pipe fitting;31, clamping groove;32, limit ring;33, gear ring;4, driving part;5, stirring pipe;51, sleeve seat;52, clamping ring seat;53, sealing element;6, circulating pump;7, delivery pipe;8, second pipe fitting;81, first butt joint pipe;82, telescopic pipe;83, second butt joint pipe. DETAILED DESCRIPTION
[0028] The technical scheme of the utility model will be described clearly and completely below in connection with the drawings in the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model and not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.
[0029] In the description of the utility model, it needs to be explained that the orientation or position relation indicated by the terms "intermediate", "upper", "lower", "left", "right", "inner", "outer" and the like is the orientation or position relation based on the shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the utility model.
[0030] As Figure 1 The specific scheme of the embodiment is as follows: an agarose cook glue device, including heating oil tank 1, the heating oil tank 1 is matched with cook glue barrel 2, the cook glue barrel 2 includes barrel body 21 and the barrel cover 22 of the barrel body 21 top end, the inside of heating oil tank 1 is provided with the first pipe fitting 3 of extending out and the driving part 4 of driving first pipe fitting 3 rotation, the inside of barrel body 21 is equipped with the stirring pipe 5 of extending to the bottom of barrel body 21, and stirring pipe 5 is connected with first pipe fitting 3, and the center of barrel cover 22 is provided with the transition pipe 221 of detachable butt joint with stirring pipe 5, and the circulating pump 6 of extracting hot oil in heating oil tank 1 is arranged on heating oil tank 1, and the delivery pipe 7 is arranged on the output end of circulating pump 6, and the second pipe fitting 8 of the movable connection with transition pipe 221 is arranged on the other end of delivery pipe 7.
[0031] AsFigure 1 As shown, in use, the first pipe 3 is driven to rotate by the driving member 4, and the stirring pipe 5 connected with the first pipe 3 rotates synchronously. During the rotation of the stirring pipe 5, the circulating pump 6 is used to keep the high-temperature hot oil flowing in the stirring pipe 5, so that the stirring pipe 5 is heated by the high-temperature hot oil to heat the solution with agarose in the barrel 21. Since the stirring pipe 5 is continuously rotating, the stirring pipe 5 can quickly and uniformly transfer heat to the solution mixture in the barrel 21, thereby achieving uniform heating and effectively ensuring the gel quality and improving the accuracy of the electrophoresis result.
[0032] Preferably, a temperature measuring device such as an electronic thermometer can be arranged on the barrel 21 to continuously monitor the temperature of the solution in the barrel 21, so that the operator can better control the on-off time of the circulating pump 6.
[0033] As shown in the drawings, Figure 1 In the embodiment, the heating wire 11 is arranged in the heating oil tank 1 to heat the oil in the heating oil tank 1. The controller 12 is arranged on one side of the heating oil tank 1 to control the on-off and operation time of the heating wire 11, the driving member 4 and the circulating pump 6. The heating oil tank 1 is provided with a placing groove 13 on the top thereof, which is adapted to the barrel 21 for conveniently placing the gel boiling barrel 2, and the first pipe 3 is located at the center of the placing groove 13.
[0034] It should be noted that in the embodiment, the heating oil tank 1 is designed as an "L" shape, the barrel 21 is placed on the high top surface of the heating oil tank 1, and the circulating pump 6 is arranged at the low top surface of the heating oil tank 1. The heating oil tank 1 is filled with heating oil, which is used as a medium to heat the stirring pipe 5, thereby achieving the purpose of heating the solution.
[0035] In addition, the liquid level of the heating oil is lower than the bottom surface of the driving member 4 to avoid the heating oil contacting the driving member 4.
[0036] As shown in the drawings, Figure 1 , Figure 5 , Figure 7 The barrel 21 is provided with an embedded groove 211 on the bottom wall thereof, which is adapted to the first pipe 3, and the bottom end of the stirring pipe 5 extends into the embedded groove 211. The embedded groove 211 enables the stirring pipe 5 to be more stably installed in the barrel 21 and tightly cooperates with the first pipe 3, thereby ensuring that the hot oil can smoothly flow into the stirring pipe 5 for heating.
[0037] In the embodiment, the stirring pipe 5 is rotatably connected with the bottom wall of the barrel 21 through the sealing member 53, which ensures that the stirring pipe 5 does not leak hot oil during rotation. The sealing member 53 can be a sealing bearing, which is embedded into the bottom wall of the barrel 21, and the stirring pipe 5 penetrates the inner ring of the sealing bearing.
[0038] The outer periphery of the bottom end of the stirring pipe 5 and inside the inner embedding groove 211 are provided with a clamping ring seat 52 which is clamped with the top end of the first pipe 3, through the design of the clamping ring seat 52, the stirring pipe 5 and the first pipe 3 are clamped with each other, so that the first pipe 3 can drive the stirring pipe 5 to rotate when the first pipe 3 rotates.
[0039] In this embodiment, the inner top wall of the heating oil tank 1 is provided with a placing box, the first pipe 3 and the driving part 4 are arranged in the placing box, the first pipe 3 vertically penetrates the placing box, and the first pipe 3 is rotatably connected with the heating oil tank 1 and the placing box. The placing box is made of heat insulation and oil-proof material, which can isolate high temperature and prevent oil from adhering to the placing box for a long time.
[0040] The driving part 4 comprises a motor, and a driving gear connected with the first pipe 3 is arranged on the output shaft of the motor.
[0041] In this embodiment, the top end of the first pipe 3 is provided with a clamping groove 31 matched with the clamping ring seat 52, the outer periphery of the first pipe 3 is provided with a limiting ring 32 rotatably connected with the inner bottom wall of the placing box, and the surface of the first pipe 3 is provided with a gear ring 33 engaged with the driving gear.
[0042] As shown in Figure 2 , Figure 3 , Figure 8 , the top end of the first pipe 3 is provided with a clamping groove 31 clamped with the stirring pipe 5, the first pipe 3 is rotatably connected with the heating oil tank 1, the surface of the first pipe 3 is provided with a limiting ring 32 rotatably connected with the inner bottom wall of the placing box, and the surface of the first pipe 3 inside the placing box is provided with a gear ring 33 engaged with the driving gear.
[0043] Through the above technical scheme, the design of the clamping groove 31 and the gear ring 33 makes the stirring pipe 5 firmly clamped with the first pipe 3, and the stirring function is realized through the rotation of the driving gear. The limiting ring 32 ensures the stability of the first pipe 3 during rotation.
[0044] As shown in Figure 1 , Figure 4 , Figure 7 , the stirring pipe 5 adopts a serpentine pipe, and other shaped pipes can also be used to increase the stirring area and improve the stirring efficiency. The top end and the bottom end of the stirring pipe 5 are on the axis of the barrel 21, and the top end of the stirring pipe 5 is provided with a sleeve seat 51 movably connected with the transition pipe 221. The design of the sleeve seat 51 makes the stirring pipe 5 firmly and stably connected with the transition pipe 221 and the first pipe 3.
[0045] As shown in Figure 6 , Figure 9As shown, the second pipe 8 includes a first adapter pipe 81 connected with the delivery pipe 7, the other end of the first adapter pipe 81 is provided with an extension pipe 82, the other end of the extension pipe 82 is provided with a second adapter pipe 83 movably connected with the transition pipe 221, and the extension pipe 82 is telescopic, so that the second pipe 8 can be movably connected with the transition pipe 221.
[0046] As shown, Figure 6 In this embodiment, the transition pipe 221 is a sleeve with a "T" shaped cross section, the thick section of the transition pipe 221 is located on the top of the barrel cover 22, the bottom end of the second adapter pipe 83 is movably connected with the thick section of the transition pipe 221, and the thin section of the transition pipe 221 passes through the barrel cover 22, and the bottom end of the thin section of the transition pipe 221 is movably connected with the sleeve seat 51.
[0047] When the barrel cover 22 is covered on the barrel body 21, the transition pipe 221 is inserted into the sleeve seat 51, and the transition pipe 221 is movably connected with the sleeve seat 51. In order to prevent the hot oil from leaking when the hot oil is poured into the stirring pipe 5, the inner diameter of the transition pipe 221 is less than or equal to the inner diameter of the sleeve seat 51, so that the hot oil output by the transition pipe 221 can be completely accepted by the stirring pipe 5.
[0048] The working principle of the above embodiment is as follows: during operation, first, the gel boiling barrel 2 filled with a specific buffer solution containing agarose is placed on the placing groove 13 on the top of the hot oil tank 1, so that the top end of the first pipe 3 vertically extending in the hot oil tank 1 is inserted into the embedded groove 211 in the bottom wall of the barrel body 21 of the gel boiling barrel 2, at this time, the bottom end of the stirring pipe 5 is clamped with the first pipe 3 through the snap ring seat 52. Then, the heating wire 11 in the hot oil tank 1 is used to heat the oil, and the controller 12 is used to set the working parameters of the heating wire 11, the motor of the driving member 4 and the circulating pump 6.
[0049] When the hot oil reaches the set temperature, the controller 12 starts the driving member 4, and the motor drives the first pipe 3 and the stirring pipe 5 clamped with the first pipe 3 to rotate synchronously through the driving gear. At this time, the circulating pump 6 starts to work, and the high-temperature hot oil in the hot oil tank 1 is pumped into the second pipe 8 through the delivery pipe 7, and the second pipe 8 is movably connected with the transition pipe 221 in the center of the barrel cover 22 of the gel boiling barrel 2, so that the hot oil is sent into the stirring pipe 5, and then reenters the hot oil tank 1.
[0050] The hot oil continuously flows in the stirring pipe 5, so that the stirring pipe 5 is heated to become a heating member, and the solution with agarose in the barrel body 21 is heated. Since the stirring pipe 5 is continuously rotating, the heat can be uniformly and quickly transferred to the solution, so that the solution is uniformly heated, thereby effectively ensuring the quality of the gel and improving the accuracy of the electrophoresis result.
[0051] During the heating process, the temperature of the solution can be continuously monitored by the electronic thermometer arranged on the barrel 21, so that the operator can better control the switching time of the circulating pump 6. When the solution reaches the required temperature, the controller 12 turns off the circulating pump 6 and the heating wire 11, and the gel boiling process is completed.
[0052] After the gel boiling is completed, the second pipe 8 is pulled upward, the telescopic pipe 82 is retracted, the second pipe 8 is separated from the transition pipe 221, and then the gel boiling barrel 2 can be taken out, and then the barrel cover 22 is opened to pour out the gel mixture in the barrel body 21.
[0053] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and modifications can be made to these embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.
Claims
1. An agarose gel cooking apparatus, characterized in that, The application relates to a heating oil tank which is provided with a glue boiling barrel, the glue boiling barrel comprises a barrel body and a barrel cover sleeved on the top end of the barrel body, a first pipe is vertically arranged in the heating oil tank and extends outwards, and a driving element is arranged in the heating oil tank and drives the first pipe to rotate, a stirring pipe is arranged in the barrel body and extends to the bottom of the barrel body, the stirring pipe is connected with the first pipe, a transition pipe is arranged in the center of the barrel cover and detachably connected with the stirring pipe, a circulating pump is arranged on the heating oil tank and draws hot oil in the heating oil tank, a conveying pipe is arranged at the output end of the circulating pump, the other end of the conveying pipe is provided with a second pipe which is movably connected with the transition pipe, an embedded groove is formed in the bottom wall of the barrel body and matched with the first pipe, the bottom end of the stirring pipe extends into the embedded groove, the stirring pipe is rotatably connected with the bottom wall of the barrel body through a sealing element, a clamping ring seat is arranged on the outer periphery of the bottom end of the stirring pipe and located in the embedded groove and matched with the top end of the first pipe, the driving element comprises a motor, and a driving gear is arranged on the output shaft of the motor and connected with the first pipe.
2. The apparatus for cooking gel according to claim 1, wherein: A heating wire is arranged in the heating oil tank, a controller is arranged on one side of the heating oil tank, a placing groove is formed in the top of the heating oil tank and matched with the barrel body, and the first pipe is located in the center of the placing groove.
3. The apparatus of claim 1, wherein: A placing box is arranged on the inner top wall of the heating oil tank, the first pipe and the driving element are arranged in the placing box, the first pipe vertically penetrates through the placing box, and the first pipe is rotatably connected with the heating oil tank and the placing box.
4. The apparatus according to claim 3, wherein: A clamping groove is formed in the top end of the first pipe and matched with the clamping ring seat, a limiting ring is arranged on the outer periphery of the first pipe and rotatably connected with the bottom wall in the placing box, and a gear ring is arranged on the surface of the first pipe and engaged with the driving gear.
5. The apparatus of claim 1 wherein: the apparatus further comprises a heating element; and the heating element is positioned to heat the gelatinous substance. The second pipe comprises a first connecting pipe connected with the conveying pipe, a telescopic pipe is arranged at the other end of the first connecting pipe, and a second connecting pipe is arranged at the other end of the telescopic pipe and movably connected with the transition pipe. 6. An agarose gel cooking apparatus as claimed in claim 5, characterized in that: The transition pipe is a sleeve pipe with a T-shaped cross section, the thick section of the transition pipe is located on the top of the barrel cover, the bottom end of the second connecting pipe is movably connected with the thick section of the transition pipe, the thin section of the transition pipe penetrates through the barrel cover, and the bottom end of the thin section of the transition pipe is movably connected with the sleeve seat.