Paramecium avoidance reaction detection device

Abstract

The utility model relates to the technical field of biological experiment equipment, in particular to a paramecium avoidance reaction detection device which comprises a placement box and a detection plate, the placement box comprises a box body, a drawer structure, a drainage structure, a temperature control system, a humidity control system, an illumination system and a control panel, and the drawer structure is located on the right side of the placement box; the drainage structure comprises a plurality of stand columns located in all the drawer structures and a water tank communicated with all the drawer structures, and the upper surfaces of all the stand columns are located on the same plane and jointly form a placement plane of the detection plate; the temperature control system comprises a heating device and a temperature sensor; the humidity control system comprises a humidifier and a humidity sensor; the detection plate comprises a chemotactic reaction detection plate and an avoidance reaction detection plate; according to the device, strict control of a single variable during an experiment is realized, and the accuracy of an experiment result can be improved; the placement box can maintain a good physiological state of paramecium while reducing intermolecular acting force between the detection plate and a placement plane, and the success rate of an experiment is improved.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, specifically to a device for detecting the avoidance response of paramecium. Background Technology

[0002] Paramecium's avoidance response is a stress response to external stimuli. Studying paramecium's avoidance response not only helps to understand the adaptation mechanisms of organisms to the environment, but also provides important experimental materials and theoretical basis for studying the stress response and survival strategies of organisms. In environmental science, the avoidance response of paramecium can be used to quickly monitor environmental pollution and determine the ecotoxicity of chemical substances.

[0003] Currently, paramecium avoidance experiments are typically performed on a glass slide. Two drops of low-concentration paramecium culture medium are placed on the slide, and a dissecting needle is used to connect the two drops using a scribbling line. At the start of the experiment, a chemical substance is added to one side of the culture medium, and a certain number of paramecia are inoculated. The number of paramecia that avoid the other droplet at different times is counted under a microscope, allowing the calculation of the paramecium avoidance rate. Alternatively, if a chemical substance is added to one droplet and paramecia are inoculated on the other, the number of paramecia swimming to the droplet containing the chemical substance can be counted, allowing the calculation of the paramecium chemotaxis rate.

[0004] However, using glass slides for paramecium repulsion experiments has certain drawbacks: when adding culture medium to the slide, it is impossible to guarantee that the two drops of culture medium will have the same shape and area on the slide; furthermore, when streaking between two drops of culture medium, it is impossible to guarantee that the width and length of the channel will be uniform. Different areas of culture medium droplets and channels of different lengths and widths will cause significant deviations in the results of repeated experiments in each group.

[0005] In addition, to ensure the physiological state of the paramecia during the experiment and to prevent the solution on the slide from drying out, it is necessary to keep the temperature, humidity and light conditions constant. After the addition and streaking operations are completed, the slide needs to be moved into a constant temperature and humidity chamber. At the same time, when observing the results, the slide also needs to be removed and placed under a microscope. However, there are often certain intermolecular forces between the slide and the surface on which it is placed. During the movement, the solution may mix, which may affect the accuracy of the experimental data.

[0006] Therefore, a device for detecting the paramecium's avoidance response is needed that can ensure the consistency of irrelevant variables during the experiment and eliminate the influence of intermolecular forces between the slide and the placement plane on the experimental operation. Utility Model Content

[0007] This invention provides a device for detecting the attraction and avoidance response of Paramecium, which can ensure the consistency of irrelevant variables during the experiment and eliminate the influence of intermolecular forces between the slide and the placement plane on the experimental operation.

[0008] To solve the above problems, the present invention provides a paramecium repulsion detection device with the following technical solution:

[0009] A device for detecting the repulsion response of paramecia includes a placement box and a detection plate. The placement box includes a box body, multiple drawer structures, a drainage structure, a temperature control system, a humidity control system, a lighting system, and a control panel. The drawer structures are located on the right side of the placement box and are used to place the detection plate. The drainage structure includes multiple uprights located within each drawer structure and a water tank communicating with each drawer structure. The upper surfaces of each upright are on the same plane and together form the placement plane of the detection plate. The temperature control system includes a heating device and a temperature sensor. The humidity control system includes a humidifier and a humidity sensor. The heating device, humidifier, temperature sensor, and humidity sensor are all electrically connected to the control panel.

[0010] The detection plate includes a chemotactic reaction detection plate and an avoidance reaction detection plate. The chemotactic reaction detection plate has an experimental cell, and multiple reaction cells are evenly distributed around the experimental cell. There are through grooves between the experimental cell and each reaction cell to connect the experimental cell and the reaction cells. The avoidance reaction detection plate has two placement slots, and there is a connecting groove between the two placement slots.

[0011] This invention relates to a paramecium chemotaxis and avoidance reaction detection device. By replacing the glass slide with chemotaxis and avoidance reaction detection plates, the shape and size of the droplets, as well as the length and width of the channels, can be kept consistent in the chemotaxis and avoidance reaction experiments. This achieves strict control of a single variable, thereby improving the accuracy of the experimental results. In addition, the temperature control, humidity control, and light control systems in the placement box maintain the paramecium in a good physiological state, increasing the success rate of the experiment. Furthermore, the column design reduces the intermolecular forces between the detection plates and the placement plane, thus minimizing their impact on the experimental results.

[0012] Furthermore, the heating device is a water bath heater, which is installed on the rear side of the placement box.

[0013] Furthermore, the lighting system includes an LED light strip installed on the rear side of the placement box to provide lighting conditions for the experiment.

[0014] Furthermore, each of the drawer structures includes a bottom plate, a left side plate, and a right side plate. The temperature sensor and humidity sensor are both mounted on the right side plate. The upper surface of the bottom plate is an inclined surface that slopes from top to bottom and to the left. The left side plate has a drain outlet.

[0015] Furthermore, the water tank is located on the left side of each drawer structure, and the upper side of the water tank has a water guide pipe that is connected to each drain outlet.

[0016] Furthermore, the humidity control system also includes a water distribution network located between the humidifier and each drawer structure, for uniformly dispersing water vapor within each drawer structure.

[0017] Furthermore, each of the drawer structures has a structural plate fixed to the box body on its upper side. The structural plate includes a support plate, a water-absorbing plate, and a fixing net from top to bottom. The support plate is used to support the upper structure. The water-absorbing plate is made of water-absorbing material and is used to prevent water vapor from condensing into water droplets. The water-absorbing plate is detachably installed on the support plate through the fixing net.

[0018] Furthermore, a liquid-sparing ring is movably mounted on the chemotactic reaction detection plate, and a liquid-sparing plate is movably mounted on the avoidance reaction detection plate. Both the liquid-sparing ring and the liquid-sparing plate are used to separate the solutions on both sides of the plate.

[0019] The beneficial effects of the paramecium repulsion detection device provided by this utility model are:

[0020] 1. The Paramecium chemotaxis and avoidance reaction detection device of this invention replaces the glass slide by setting up chemotaxis and avoidance reaction detection plates, so that the droplet shape, size, channel length and width can be kept consistent in the chemotaxis and avoidance reaction experiment, achieving strict control of a single variable and thus improving the accuracy of experimental results. In addition, the temperature control system, humidity control system and light system set in the placement box can maintain the good physiological state of Paramecium and improve the success rate of the experiment. Furthermore, the setting of the column can reduce the intermolecular forces between the detection plate and the placement plane, thereby reducing the impact on the experimental results.

[0021] 2. By installing absorbent plates on the upper side of each drawer structure, water vapor can be prevented from condensing into water droplets on the upper side of the drawer structure, thus preventing water droplets from falling onto the test plate and improving the accuracy of the experimental results. Attached Figure Description

[0022] The above and other objects, features, and advantages of the present invention will become readily understood by reading the following detailed description of exemplary embodiments with reference to the accompanying drawings. In the drawings, several embodiments of the present invention are shown by way of example and not limitation, and like or corresponding reference numerals denote like or corresponding parts, wherein:

[0023] Figure 1 A partial cross-sectional view of a paramecium repulsion detection device provided by this utility model;

[0024] Figure 2Rear view of a paramecium repulsion detection device provided by this utility model;

[0025] Figure 3 for Figure 1 Schematic diagram of the structure of a chemotactic reaction detection plate;

[0026] Figure 4 for Figure 1 Schematic diagram of the structure of the avoidance reaction detection plate;

[0027] Figure 5 for Figure 3 Schematic diagram of the liquid ring structure in the middle partition;

[0028] Figure 6 for Figure 4 A schematic diagram of the structure of the liquid separator plate.

[0029] Explanation of reference numerals in the attached figures:

[0030] 1. Storage box; 11. Box body; 12. Drawer structure; 121. Left side panel; 122. Right side panel; 123. Bottom panel; 124. Drain outlet; 125. Handle; 126. Foot pad;

[0031] 13. Drainage structure; 131. Column; 132. Water tank; 133. Water pipe; 14. Temperature control system; 141. Water bath heater; 142. Temperature sensor; 15. Humidity control system; 151. Humidifier; 152. Humidity sensor; 153. Water distribution network; 16. Lighting system; 161. LED light strip; 17. Control panel;

[0032] 2. Detection plate; 21. Chemotactic reaction detection plate; 211. Experimental tank; 212. Reaction tank; 213. Through channel; 22. Avoidance reaction detection plate; 221. Placement tank; 222. Groove; 223. Liquid separator ring; 224. Baffle; 225. Liquid separator plate; 226. Partition plate. Detailed Implementation

[0033] The principles and spirit of this utility model will be explained in detail below with reference to several representative embodiments.

[0034] Example 1 of the Paramecium repulsion detection device provided by this utility model:

[0035] like Figures 1 to 6As shown, the paramecium repulsion detection device includes a placement box 1 and a detection plate 2. The placement box 1 includes a box body 11, drawer structures 12, a drainage structure 13, a temperature control system 14, a humidity control system 15, a lighting system 16, and a control panel 17. The box body 11 is a rectangular box with feet 126 at the bottom to prevent sliding between the box body 11 and the placement surface. There are three drawer structures 12 arranged vertically on the right side of the box body 11. The drawer structures 12 are slidably installed on the box body 11 in the left-right direction and are used to place the detection plate 2. The drainage structure 13 is used to drain moisture from the drawer structures 12. The temperature control system 14 and the humidity control system 15 are used to adjust the temperature and humidity inside the box body 11 under the control of the control panel 17.

[0036] The drawer structure 12 will be introduced first below, such as Figure 1 As shown, the drawer structure 12 includes a left side panel 121, a right side panel 122, and a bottom panel 123. The bottom panel 123 is a rectangular panel, and its upper surface is a sloping surface that slopes from top to bottom and to the left, so that water vapor condensation flows to the left side of the bottom panel 123. The left side panel 121 is located on the left side of the bottom panel 123 and is fixedly connected to it. The lower end of the left side panel 121 has a drain outlet 124 extending to the left and right for draining water from inside the drawer structure 12. The right side panel 122 is located on the right side of the bottom panel 123 and is fixedly connected to it. The right side of the right side panel 122 has a handle 125 for the operator to apply force.

[0037] The drainage structure 13 is described below, such as Figure 1 As shown, the drainage structure 13 includes multiple vertically extending columns 131 and a water tank 132 evenly distributed on the upper side of the base plate 123. The upper surfaces of each column 131 are located on the same plane and together form the placement plane of the detection plate 2, thereby reducing the intermolecular forces between the detection plate 2 and the placement plane. The water tank 132 is a rectangular transparent graduated box, located at the lower left of the box body 11 and slidably installed inside the box body 11. The upper end of the water tank 132 has vertically extending water guide pipes 133, which are connected to each drain port 124 to drain the water in the drawer structure 12 into the water tank 132.

[0038] The humidity control system 15 is described below, such as... Figure 1As shown, the humidity control system 15 includes a humidifier 151 and three humidity sensors 152, each mounted on the left side of a right-side panel 122. The humidifier 151 is fixedly mounted on the housing 11 and positioned above the water tank 132. Both the humidifier 151 and each humidity sensor 152 are connected to the control panel 17 via wires to maintain the humidity inside the housing 11 between 50% and 60%. A vertically extending water distribution mesh 153, fixedly mounted on the housing 11, is also provided between the humidifier 151 and each drawer structure 12. The water distribution mesh 153 is used to evenly distribute water vapor within each drawer structure 12.

[0039] The temperature control system 14 is described below, such as Figure 1 and Figure 2 As shown, the temperature control system 14 includes a heating device and a temperature sensor 142. There are three temperature sensors 142 installed on the left side of each right side panel 122. The heating device is a water bath heater 141, which is located on the rear side of the chamber 11. The water bath heater 141 and the temperature sensor 142 are both connected to the control panel 17 by wires to maintain the temperature inside the chamber 11 between 23°C and 27°C.

[0040] The following describes the lighting system 16, such as... Figure 2 As shown, the lighting system 16 includes an LED light strip 161 installed on the rear side of the housing 11, which is used to provide the light required for the growth and division of paramecia.

[0041] The following describes detection plate 2, such as... Figures 3 to 6 As shown, the detection plate 2 includes a chemotactic response detection plate 21 and an avoidance response detection plate 22, both made of transparent acrylic sheets. The chemotactic response detection plate 21 is a circular plate with a diameter of 4.5 cm, and has a recessed, circular experimental groove 211 at its center. The experimental groove 211 is used to hold Paramecium culture medium. Four circular reaction grooves 212 are evenly distributed around the outer side of the experimental groove 211. The size of the reaction grooves 212 is the same as that of the experimental groove 211. Each experimental groove 211 and each reaction groove 212 has a through-slot 213 connecting the experimental groove 211 and the reaction grooves 212, allowing Paramecium to pass through. A circular liquid-separating ring 223 is movably mounted on the chemotactic reaction detection plate 21. The lower side of the liquid-separating ring 223 has a semi-circular baffle 224 corresponding to each through groove 213. The shape of the baffle 224 is adapted to the shape of the through groove 213 and is used to separate the solutions on both sides of the baffle 224.

[0042] The avoidance reaction detection plate 22 is a rectangular plate with dimensions of 8 cm by 8 cm. It has five linearly arranged placement grooves 221. Each group of placement grooves 221 includes two concave circular placement grooves 221 with a radius of 0.6 cm and a depth of 0.4 cm. A connecting groove 222, 2 cm long and 0.3 cm deep, is provided between the two placement grooves in each group for the paramecium to pass through. A vertically extending rectangular liquid-separating plate 225 is movably mounted on the avoidance reaction detection plate 22. The lower side of the liquid-separating plate 225 corresponds to each groove 222 and has a semi-circular partition 226. The shape of the partition 226 matches the cross-sectional shape of the groove 222, separating the solutions on both sides of the partition 226.

[0043] The working principle of a paramecium repulsion response detection device is summarized as follows:

[0044] First, place multiple sets of detection plates 2 on the uprights 131 inside the drawer structure 12. Install the liquid-separating ring 223 / liquid-separating plate 225 on the tank / groove 222. Then, add experimental culture medium or solution to the experimental tank 211 / placement tank 221 / reaction tank 212 of the detection plate 2. Adjust the temperature, humidity, and light conditions inside the placement box 1 using the control panel 17 to ensure the physiological state of the paramecium. Then, remove the liquid-separating ring 223 / liquid-separating plate 225 and begin the experiment. The temperature sensor 142 and humidity sensor 152 inside the placement box 1 ensure a constant temperature and humidity inside the box 11, preventing the experimental solution from drying out. After a certain time, the detection plates 2 can be removed and placed under a microscope for observation and recording.

[0045] Example 2 of the Paramecium repulsion detection device provided by this utility model:

[0046] Its main difference from Example 1 is:

[0047] In Example 1, the heating device is a water bath heater.

[0048] In this embodiment, the heating device is an electric heating wire evenly distributed on the side wall of the box.

[0049] Based on the above description in this specification, those skilled in the art will also understand that the following terms used, such as "upper," "lower," "front," "rear," "left," "right," "width," "horizontal," "top," "bottom," "inner," and "outer," are terms indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings of this specification. They are only for the purpose of facilitating the explanation of the present invention and simplifying the description, and do not explicitly or implicitly suggest that the device or element involved must have the specific orientation, or be constructed and operated in a specific orientation. Therefore, the above-mentioned orientation or positional relationship terms should not be understood or interpreted as limitations on the present invention.

[0050] In addition, in the description of this specification, "multiple" means at least two, such as two, three or more, etc., unless otherwise expressly and specifically defined.

Claims

1. A paramecium avoidance reaction detection device comprising a placement case and a detection plate, characterized by, The placing box comprises a box body, a plurality of drawer structures, a drainage structure, a temperature control system, a humidity control system, an illumination system and a control panel. The drawer structures are located on the right side of the placing box and used for placing detection plates. The drainage structure comprises a plurality of vertical columns located in each drawer structure and a water tank communicated with each drawer structure. The upper surfaces of the vertical columns are located on the same plane and jointly form a placing plane of the detection plates. The temperature control system comprises a heating device and a temperature sensor. The humidity control system comprises a humidifier and a humidity sensor. The heating device, the humidifier, the temperature sensor and the humidity sensor are electrically connected with the control panel. The detection plates comprise chemotaxis reaction detection plates and avoidance reaction detection plates. The chemotaxis reaction detection plate has an experimental groove. A plurality of reaction grooves are uniformly distributed on the outer side of the experimental groove. The experimental groove and each reaction groove have a through groove for communicating the experimental groove and the reaction groove. The avoidance reaction detection plate has two placing grooves. The two placing grooves have a communicating groove.

2. The Paramecium avoidance reaction detection device according to claim 1, wherein The heating device is a water bath heater installed on the rear side of the placing box.

3. The Paramecium avoidance reaction detection device according to claim 1, wherein The illumination system comprises LED lamp strips installed on the rear side of the placing box and used for providing illumination conditions for experiments.

4. The Paramecium avoidance reaction detection device according to claim 1, wherein Each drawer structure comprises a bottom plate, a left side plate and a right side plate. The temperature sensor and the humidity sensor are installed on the right side plate. The upper surface of the bottom plate is an inclined surface inclined to the left from top to bottom. The left side plate has a drainage port.

5. The Paramecium avoidance reaction detection device according to claim 4, wherein The water tank is located on the left side of each drawer structure. The upper side of the water tank has a water guide pipe communicated with each drainage port.

6. The Paramecium avoidance reaction detection device according to claim 1, wherein The humidity control system further comprises a water distribution net located between the humidifier and each drawer structure and used for uniformly distributing water vapor in each drawer structure.

7. The Paramecium avoidance reaction detection device according to claim 1, wherein The upper side of each drawer structure has a structural plate fixed on the box body. The structural plate comprises, in sequence from top to bottom, a support plate, a water absorption plate and a fixing net. The support plate is used for supporting an upper layer structure. The water absorption plate is made of a water absorption material and used for preventing water vapor from condensing into water droplets. The water absorption plate is detachably installed on the support plate through the fixing net.

8. The Paramecium avoidance reaction detection device according to claim 1, wherein The chemotaxis reaction detection plate movably has a liquid separation ring. The avoidance reaction detection plate movably has a liquid separation plate. The liquid separation ring and the liquid separation plate are used for separating solutions on both sides thereof.

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