Cooling device used in stem cell cryopreservation process

Abstract

The utility model discloses a cooling device used in a stem cell cryopreservation process, and relates to the technical field of stem cell cryopreservation equipment. The device comprises a storage box, a cover plate is hinged to one side of the top of the storage box, a placement box is installed in the storage box, a plurality of test tube grooves are formed in the top of the placement box at equal intervals, and a gap groove is formed between the placement box and the inner wall of the storage box. Dry ice storage boxes are fixedly installed on the two opposite sides of the outer wall of the storage box, conveying pipes at the bottoms of the dry ice storage boxes are inserted into the bottoms of the gap grooves, and baffles distributed at intervals are fixedly arranged in the gap grooves. The cooling box, the water inlet pipe and the water outlet pipe can block external high temperature through circularly-cooled cooling water, a proper low-temperature environment in the transfer box is maintained, adverse effects of the external environment on the cooling process are reduced, normal cooling work is guaranteed, and the overall cooling efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of stem cell cryopreservation equipment technology, specifically to a cooling device used in the stem cell cryopreservation process. Background Technology

[0002] Stem cells are a type of pluripotent cell with self-renewal capacity. Under certain conditions, they can differentiate into various APSC pluripotent cells. They are a type of primitive cell with self-replication and multi-directional differentiation potential. After collection and processing, stem cells are placed in a transport box and then transported into a liquid nitrogen storage tank for cryopreservation during a slow cooling process.

[0003] Currently, when cooling is performed during stem cell transport, the surface of the transport box gradually heats up due to interference from the external high-temperature environment. This leads to a decrease in the cooling effect during the cooling process, ultimately affecting the overall cooling efficiency and requiring more time to transport the stem cells to the liquid nitrogen storage tank. Utility Model Content

[0004] In order to solve the above problems, the purpose of this utility model is to provide a cooling device used in the process of stem cell cryopreservation.

[0005] To solve the above technical problems, the present invention adopts the following technical solution: a cooling device used in the cryopreservation of stem cells, including a storage box, a cover plate hinged to one side of the top of the storage box, a placement box installed inside the storage box, a plurality of test tube slots equally spaced on the top of the placement box, a gap groove formed between the placement box and the inner wall of the storage box, dry ice storage boxes fixedly installed on opposite sides of the outer wall of the storage box, a bottom delivery pipe of the dry ice storage box inserted into the bottom of the gap groove, baffles fixedly arranged at intervals in the gap groove, adjacent baffles arranged diagonally from top to bottom, a cooling tank opened inside the storage box, the cooling tank surrounding the placement box, a water outlet pipe connected to one of the adjacent dry ice storage boxes on the outside of the storage box via a pump body, a cooling box fixedly installed at the bottom of the outer wall of the storage box, the bottom of the water outlet pipe connected to the cooling box, a water inlet pipe connected to the bottom of the cooling box, the side of the water inlet pipe away from the cooling box inserted into the cooling tank.

[0006] Preferably, the placement box has grooves on both sides inside the test tube slot, a spring is fixedly installed in the groove, and a positioning block is fixedly connected to the end of the spring away from the groove. The positioning block is arc-shaped.

[0007] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0008] 1. In this utility model, the cooling box, inlet pipe and outlet pipe can block the external high temperature through the circulating cooling water, maintain a suitable low temperature environment inside the transfer box, reduce the adverse effects of the external environment on the cooling process, ensure the normal operation of the cooling work, and improve the overall cooling efficiency.

[0009] 2. The dry ice storage box of this utility model delivers dry ice into the gap groove through the delivery pipe. The baffle in the gap groove makes the gas flow in an up-down path, so that it can pass evenly over the surface of the box. Thus, during the transportation process, the stem cells in the test tube can be cooled slowly and evenly, which is conducive to maintaining the stability of the temperature of the environment in which the stem cells are located and ensuring their quality.

[0010] 3. In this utility model, the spring is used to push the positioning block to limit the test tube, ensuring stable positioning. When there is external shaking, the spring can buffer it and ensure its safety. Attached Figure Description

[0011] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0013] Figure 2 This is a schematic diagram of the internal structure of the storage box of this utility model.

[0014] Figure 3 This is a top view of the storage box structure of this utility model.

[0015] Figure 4 This is a front view structural diagram of the placement box of this utility model.

[0016] Figure 5 This utility model Figure 4 Enlarged structural diagram at point A in the middle.

[0017] In the diagram: 1. Storage box; 11. Cover plate; 12. Placement box; 13. Test tube trough; 14. Groove; 15. Spring; 16. Positioning block; 17. Protective pad; 18. Crossbar; 19. Sealing gasket; 191. Sealing groove; 2. Dry ice storage box; 21. Delivery pipe; 22. Gap groove; 23. Baffle; 3. Cooling box; 31. Water outlet pipe; 32. Water inlet pipe; 33. Cooling trough; 34. Heat dissipation fins. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Example: Figure 1-5 As shown, this utility model provides a cooling device used in the cryopreservation of stem cells, including a storage box 1. A cover plate 11 is hinged to one side of the top of the storage box 1. A placement box 12 is installed inside the storage box 1. Multiple test tube slots 13 are opened at equal intervals on the top of the placement box 12. A gap groove 22 is formed between the placement box 12 and the inner wall of the storage box 1. Dry ice storage boxes 2 are fixedly installed on opposite sides of the outer wall of the storage box 1. The bottom delivery pipe 21 of the dry ice storage box 2 is inserted into the bottom of the gap groove 22. The delivery pipe 21 is connected to the dry ice storage box 2 by an exhaust valve. A cooling tank 33 is opened inside the storage box 1 and is arranged around the storage box 12. A water outlet pipe 31 is connected to one of the adjacent sides of the two dry ice storage boxes 2 outside the storage box 1 through a pump body. A cooling box 3 is fixedly installed at the bottom of the outer wall of the storage box 1. The bottom of the water outlet pipe 31 is connected to the cooling box 3. A water inlet pipe 32 is connected to the bottom of the cooling box 3. The side of the water inlet pipe 32 away from the cooling box 3 is inserted into the cooling tank 33.

[0020] The gap groove 22 is fixed with baffles 23 arranged at intervals. The two adjacent baffles 23 are arranged diagonally from top to bottom. The baffles 23 allow the gas to flow up and down, so that it passes evenly over the surface of the placement box 12, thereby improving the uniformity of cooling.

[0021] The lower surface of the cooling tank 3 is fixedly equipped with heat dissipation fins 34. The section of the water inlet pipe 32 located outside the cooling tank 3 is inserted into the heat dissipation fins 34. The heat dissipation fins 34 can cool the cooling water that is subsequently circulated, thereby ensuring that the cooling water in the cooling tank 33 has good cooling effect and achieving the effect of blocking the external high temperature. The section of the water inlet pipe 32 located inside the heat dissipation fins 34 is arranged in an S-shape. The S-shaped arrangement of the water inlet pipe 32 can increase the cooling water flow time, thereby increasing the cooling time and improving the cooling effect.

[0022] The placement box 12 has grooves 14 on both sides inside the test tube slot 13. A spring 15 is fixedly installed in the groove 14. A positioning block 16 is fixedly connected to the end of the spring 15 away from the groove 14. The positioning block 16 is arc-shaped. When the test tube is inserted into the test tube slot 13, the spring 15 will push the positioning block 16 to limit the test tube and ensure stable positioning. The spring 15 can buffer the test tube when it shakes externally to ensure its safety. A protective soft pad 17 is fixedly installed at the end of the positioning block 16 away from the spring 15 to avoid excessive hard contact with the test tube. A crossbar 18 is fixedly installed at the upper and lower ends of the positioning block 16 and the spring 15. The crossbar 18 can be movably inserted into the placement box 12. The crossbar 18 can ensure that the positioning block 16 can move stably and prevent it from shifting after repeated use, which would cause it to malfunction in subsequent uses.

[0023] A sealing gasket 19 is fixedly provided on the side of the cover plate 11 facing the storage box 12. A sealing groove 191 corresponding to the sealing gasket 19 is opened on the top of the inner wall of the storage box 1. The sealing gasket 19 can increase the overall sealing performance of the storage box 1 when the cover plate 11 is closed, and ensure the cooling effect. It is worth noting that there is a locking mechanism between the cover plate 11 and the storage box 1. Based on the conventional locking technology on the market, it is not shown in detail in the figure, but the storage box 1 and the cover plate 11 of this patent have a stable closure.

[0024] Working principle: During use, the operator inserts the test tube containing stem cells into the test tube slot 13 inside the placement box 12, and then closes the cover 11 directly. The dry ice storage box 2 sends dry ice into the gap 22 through the exhaust valve connected to the delivery pipe 21. The baffle 23 installed in the gap 22 allows the gas to flow up and down, thus uniformly passing over the surface of the placement box 12, thereby improving the uniformity of cooling. During the transfer process, the stem cells in the test tube are slowly cooled. The water vapor and carbon dioxide generated by the subsequent sublimation of dry ice can be discharged through the exhaust valve at the bottom. The exhaust valve is located at the four corners of the bottom of the storage box 1. Figure 3 It can be seen that after reaching the destination and meeting the required refrigeration requirements, the cells are placed in a liquid nitrogen storage tank for storage. During the transfer process, the inlet pipe 32 can inject cooling water into the cooling tank 33. After the water level gradually rises, the outlet pipe 31 draws it into the cooling box 3. Subsequently, the circulating cooling water is cooled by the heat dissipation fins 34. This ensures that the cooling water in the cooling tank 33 has good cooling effect during the transfer process, thereby blocking the external high temperature environment and ensuring that the dry ice can effectively cool the stem cells in the test tube.

[0025] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A cooling device used in stem cell cryopreservation, comprising a storage box (1), characterized in that: A cover plate (11) is hinged to one side of the top of the storage box (1). A placement box (12) is installed inside the storage box (1). The top of the placement box (12) has multiple equally spaced test tube slots (13). A gap groove (22) is formed between the placement box (12) and the inner wall of the storage box (1). Dry ice storage boxes (2) are fixedly installed on opposite sides of the outer wall of the storage box (1). The bottom delivery pipe (21) of the dry ice storage box (2) is inserted into the bottom of the gap groove (22). Inside the storage box (1) A cooling tank (33) is provided, which is arranged around the storage box (12). The outside of the storage box (1) is located on one side of the two dry ice storage boxes (2) adjacent to each other and is connected to a water outlet pipe (31) via a pump body. A cooling box (3) is fixedly provided at the bottom of the outer wall of the storage box (1). The bottom of the water outlet pipe (31) is connected to the cooling box (3). The bottom of the cooling box (3) is connected to a water inlet pipe (32). The side of the water inlet pipe (32) away from the cooling box (3) is inserted into the cooling tank (33).

2. The cooling device used in the stem cell cryopreservation process as described in claim 1, characterized in that, The gap groove (22) is fixedly provided with baffles (23) arranged at intervals, and two adjacent baffles (23) are arranged diagonally from top to bottom.

3. The cooling device used in the stem cell cryopreservation process as described in claim 1, characterized in that, The lower surface of the cooling box (3) is fixedly equipped with heat dissipation fins (34), and a section of the water inlet pipe (32) located outside the cooling box (3) is inserted into the heat dissipation fins (34).

4. The cooling device used in the stem cell cryopreservation process as described in claim 3, characterized in that, The section of the water inlet pipe (32) located inside the heat dissipation fins (34) is arranged in an S-shape.

5. The cooling device used in the stem cell cryopreservation process as described in claim 1, characterized in that, The placement box (12) has grooves (14) on both sides inside the test tube groove (13). A spring (15) is fixedly installed in the groove (14). A positioning block (16) is fixedly connected to the end of the spring (15) away from the groove (14). The positioning block (16) is arc-shaped.

6. The cooling device used in the stem cell cryopreservation process as described in claim 5, characterized in that, The end of the positioning block (16) away from the spring (15) is fixed with a protective pad (17).

7. The cooling device used in the stem cell cryopreservation process as described in claim 5, characterized in that, The positioning block (16) is fixedly provided with a crossbar (18) at the upper and lower ends of the spring (15), and the crossbar (18) can be movably inserted into the placement box (12).

8. The cooling device used in the stem cell cryopreservation process as described in claim 1, characterized in that, A sealing gasket (19) is fixedly provided on the side of the cover plate (11) facing the placement box (12), and a sealing groove (191) corresponding to the sealing gasket (19) is opened on the top of the inner wall of the storage box (1).

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