Cell treatment consumable pipe group and cell treatment equipment

Abstract

The utility model provides a cell treatment consumable pipe group and cell treatment equipment. The cell treatment consumable pipe group comprises a centrifugal container, a first pipe group, a second pipe group and a first communicating pipeline, wherein the first pipe group is communicated with the centrifugal container and used for being matched with a first driving pump; the second pipe group is communicated with the centrifugal container and used for being matched with a second driving pump; one end of the first communicating pipeline communicates with the first pipe set and is located at a first communicating point, and the other end of the first communicating pipeline communicates with the second pipe set and is located at a second communicating point. The first communication point and the centrifugal container are located on opposite sides of the first drive pump, and the second communication point and the centrifugal container are located on opposite sides of the second drive pump. According to the utility model, the pollution of cells is avoided, and meanwhile, the cell treatment efficiency is improved.

Description

Technical Field

[0001] This utility model belongs to the field of cell processing technology, and in particular relates to a cell processing consumable tube assembly and cell processing equipment. Background Technology

[0002] Currently, in the field of cell therapy, cell processing generally involves multiple procedures, such as the isolation, culture, and harvesting of PBMCs (peripheral blood mononuclear cells). In existing technologies, each of these procedures requires operators to change different equipment or tubing separately, resulting in cumbersome processes and low efficiency. Furthermore, changing equipment can easily lead to sample contamination. Summary of the Invention

[0003] This invention addresses the technical problems of low cell processing efficiency and easy contamination in the prior art by providing a cell processing consumable tube assembly and cell processing equipment.

[0004] In view of the above technical problems, this utility model provides a cell processing consumable tubing assembly, including a centrifuge container, a first tubing assembly connected to the centrifuge container and used for matching a first drive pump, a second tubing assembly connected to the centrifuge container and used for matching a second drive pump, and a first connecting pipe;

[0005] One end of the first connecting pipe is connected to the first pipe group and located at the first connection point, and the other end of the first connecting pipe is connected to the second pipe group and located at the second connection point; the first connection point and the centrifuge container are located on opposite sides of the first driving pump, and the second connection point and the centrifuge container are located on opposite sides of the second driving pump.

[0006] Optionally, the cell processing consumable tubing assembly further includes a second connecting tubing; one end of the second connecting tubing is connected to the second tubing assembly and located at a third connecting point, and the other end of the second connecting tubing is connected to the second tubing assembly and located at a fourth connecting point; the third connecting point and the centrifuge container are located on opposite sides of the second drive pump, and the fourth connecting point is located between the second drive pump and the centrifuge container.

[0007] Optionally, the cell processing consumable tubing assembly further includes a third connecting tubing; one end of the third connecting tubing is connected to the first tubing assembly and located at a fifth connecting point, and the other end of the second connecting tubing is connected to the second tubing assembly and located at a sixth connecting point; the fifth connecting point is located between the first drive pump and the centrifuge container, and the sixth connecting point is located between the second drive pump and the centrifuge container.

[0008] Optionally, the cell processing consumable tubing assembly further includes a sampling tubing for sampling; one end of the sampling tubing is connected to the second tubing assembly and located at a seventh connection point; the seventh connection point is located between the second drive pump and the centrifuge container.

[0009] Optionally, the cell processing consumable tubing assembly further includes peripheral equipment tubing for connecting peripheral devices; one end of the peripheral equipment tubing is connected to the first tubing assembly and located at an eighth connection point; the eighth connection point is located between the first drive pump and the centrifuge container.

[0010] Optionally, the first tubing assembly includes a first centrifugal line for matching the first drive pump and a first process line for connecting to a first process solution container; one end of the first centrifugal line is connected to the centrifugal container, and the other end of the first centrifugal line is connected to the end of the first process line away from the first process solution container.

[0011] Optionally, the first pipe assembly further includes a first transfer line for connecting to the first transfer liquid container; the end of the first transfer line away from the first transfer liquid container is connected to the first process line.

[0012] Optionally, the second tubing assembly includes a second centrifugal line for matching the second drive pump and a second process line for connecting to the second process solution container; one end of the second centrifugal line is connected to the centrifugal container, and the other end of the second centrifugal line is connected to the end of the second process line away from the second process solution container.

[0013] Optionally, the second tube set further includes a sample line for connecting to a sample container; one end of the second centrifuge line away from the centrifuge container is connected to the other end of the sample line away from the sample container; and / or

[0014] The second tubing assembly further includes a waste liquid line for connecting to a waste liquid container; one end of the second centrifuge line away from the centrifuge container is connected to the end of the waste liquid line away from the waste liquid container; and / or

[0015] The second pipe assembly further includes a harvesting pipe for connecting to a harvesting container, wherein the end of the second centrifugal pipe away from the centrifugal container is connected to the end of the harvesting pipe away from the harvesting container; and / or

[0016] The second tubing assembly also includes a second transfer line for connecting to the second transfer liquid container; one end of the second transfer line away from the second transfer liquid container is connected to the second process line.

[0017] A cell processing device includes a first drive pump, a second drive pump, at least one first switching valve for controlling the on / off state of a pipeline in a first pipeline group, at least one second switching valve for controlling the on / off state of a pipeline in a second pipeline group, a third switching valve for controlling the on / off state of the first connecting pipeline, and the cell processing consumable pipeline group.

[0018] The first tubing assembly is installed on the first drive pump, which is located between the first connection point and the centrifuge container; the second tubing assembly is installed on the second drive pump, which is located between the second connection point and the centrifuge container; the first tubing assembly is installed on the first switch valve, which and the centrifuge container are located on opposite sides of the first drive pump; the second tubing assembly is installed on the second switch valve, which and the centrifuge container are located on opposite sides of the second drive pump; the first connecting pipe is installed on the third switch valve.

[0019] The cell processing consumable tubing assembly provided by this utility model includes a centrifuge container, a first tubing assembly connected to the centrifuge container and used for matching a first drive pump, a second tubing assembly connected to the centrifuge container and used for matching a second drive pump, and a first connecting pipe. One end of the first connecting pipe is connected to the first tubing assembly and located at a first connection point, and the other end of the first connecting pipe is connected to the second tubing assembly and located at a second connection point. The first connection point and the centrifuge container are located on opposite sides of the first drive pump, and the second connection point and the centrifuge container are located on opposite sides of the second drive pump. The cell processing consumable tubing assembly provided by this utility model connects the first tubing assembly and the second tubing assembly to the centrifuge container respectively, and a connecting pipe is provided between the first tubing assembly and the second tubing assembly. This allows for selection based on requirements: the solution connected to the first tubing assembly is input into the centrifuge container through the second tubing assembly; the solution connected to the second tubing assembly is input into the centrifuge container through the first tubing assembly; the solution circulates within the cell processing consumable tubing assembly; or the liquid in the centrifuge container is input from different positions within the centrifuge container into different containers connected to the first or second tubing assembly. In this way, multiple processes can be completed sequentially within the cell processing consumables tubing during cell processing, eliminating the need to change equipment after each process to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency. Attached Figure Description

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Figure 1 This is a schematic diagram of the structure of a cell processing consumable tube assembly provided in one embodiment of the present invention.

[0022] Figure 2This is a schematic diagram of the structure of a cell processing device provided in one embodiment of the present invention.

[0023] The reference numerals in the accompanying drawings are as follows:

[0024] 110. Centrifuge container; 120. First tube set; 121. First centrifuge line; 122. First process line; 123. First transfer container; 124. First transfer line; 130. Second tube set; 131. Second centrifuge line; 132. Second process line; 133. Sample line; 134. Waste container; 135. Waste line; 136. Harvest line; 137. Second transfer container; 138. Second transfer line Piping; 140, First connecting pipe; 150, Second connecting pipe; 160, Third connecting pipe; 170, Sampling pipe; 180, Peripheral equipment pipe; 210, First drive pump; 220, Second drive pump; 310, First switching valve; 320, Second switching valve; 330, Third switching valve; 400, Pipe clamp; 500, Plate clamp; 600, Bubble sensor; 700, Pressure sensor; 800, Check valve. Detailed Implementation

[0025] To make the technical problems solved, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this utility model and are not intended to limit this utility model.

[0026] like Figure 1 As shown, one embodiment of this utility model provides a cell processing consumable tubing assembly, including a centrifuge container 110, a first tubing assembly 120 connected to the centrifuge container 110 and used for matching a first drive pump 210, a second tubing assembly 130 connected to the centrifuge container 110 and used for matching a second drive pump 220, and a first connecting pipe 140; one end of the first connecting pipe 140 is connected to the first tubing assembly 120 and located at a first connection point, and the other end of the first connecting pipe 140 is connected to the second tubing assembly 130 and located at a second connection point; the first connection point and the centrifuge container 110 are located on opposite sides of the first drive pump 210, and the second connection point and the centrifuge container 110 are located on opposite sides of the second drive pump 220.

[0027] Understandably, the centrifuge container 110 can be used for centrifugation or culture operations. The ends of the first tube group 120 and the second tube group 130 furthest from the centrifuge container 110 can be connected to various process solution containers (including but not limited to one or more of the following: a washing solution container for holding washing solution, an antibody container for holding antibodies, a culture medium container for holding culture medium, a separation solution container for holding separation solution, a cryopreservation solution container for holding cryopreservation solution, or a virus sample container for holding virus samples), a transfer container for holding excess PBMC solution from the centrifuge container 110 during centrifugation, a sample container for holding sample solution, a waste liquid container 134 for holding waste liquid, or a harvest container for holding cell solution that needs to be sealed after cell processing.

[0028] The first pipe assembly 120 can be a single, integral pipe assembly with the same diameter; alternatively, it can be a single or segmented pipe assembly with different diameters, so that the diameter of the portion where the first drive pump 210 is located is set according to the parameters of the first drive pump 210 to match the first drive pump 210. The second pipe assembly 130 can be a single, integral pipe assembly with the same diameter; alternatively, it can be a single or segmented pipe assembly with different diameters, so that the diameter of the portion where the second drive pump 220 is located is set according to the parameters of the second drive pump 220 to match the second drive pump 220. The connection method between the centrifuge container 110, the first pipe assembly 120, the second pipe assembly 130, and the first connecting pipe 140 includes, but is not limited to, one or more of the following: integral connection or connection via a multi-port connector. The ends of the first pipe assembly 120 and the second pipe assembly 130 that connect to the centrifuge container 110 can be located in different positions within the centrifuge container 110, for example, at the top and bottom, or in the middle and on the side, respectively.

[0029] The cell treatment consumable tubing can be equipped with, for example... Figure 2 The components shown include switching valves (first switching valve 310, second switching valve 320, and third switching valve 330, etc.) for controlling the on / off state of the pipeline, pipe clamps 400 and 500 for controlling the on / off state of the pipeline, a bubble sensor 600 for detecting the fluid flow state in the pipeline, a pressure sensor 700 for detecting the fluid pressure in the pipeline, and a first drive pump 210 and a second drive pump 220 for controlling the flow of fluid in the cell processing consumable tubing assembly, thereby achieving automated cell processing. The parameters of the above-mentioned switching valves can be set according to actual conditions; they can be the same type of switching valve or different types of switching valves.

[0030] In this embodiment, the connecting pipe connects the first tube group 120 and the second tube group 130, thereby enabling the solution connected to the first tube group 120 to be input into the centrifuge container 110 through the second tube group 130, and the solution connected to the second tube group 130 to be input into the centrifuge container 110 through the first tube group 120. Simultaneously, a circulation pipeline can be formed as needed to allow the liquid to circulate within the cell processing consumable tube group. Furthermore, the liquid in the centrifuge container 110 can also be input from different positions within the centrifuge container 110 (e.g., above or below) into different containers connected to the first tube group 120 or the second tube group 130, as needed.

[0031] The cell processing consumable tubing assembly provided by this invention connects a first tubing assembly 120 and a second tubing assembly 130 to a centrifuge container 110, and establishes a connecting pipe between the first tubing assembly 120 and the second tubing assembly 130. This allows for selective input of solutions connected to the first tubing assembly 120 into the centrifuge container 110 via the second tubing assembly 130, solutions connected to the second tubing assembly 130 into the centrifuge container 110 via the first tubing assembly 120, circulation of the solution within the cell processing consumable tubing assembly, or input of liquid from different locations within the centrifuge container 110 into different containers connected to the first tubing assembly 120 or the second tubing assembly 130. Thus, multiple processes can be sequentially completed within the cell processing consumable tubing assembly during cell processing, eliminating the need to change equipment after each process to proceed to the next, avoiding cell contamination, and improving cell processing efficiency.

[0032] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a second connecting conduit 150; one end of the second connecting conduit 150 is connected to the second tubing assembly 130 and located at a third connecting point, and the other end of the second connecting conduit 150 is connected to the second tubing assembly 130 and located at a fourth connecting point; the third connecting point and the centrifuge container 110 are located on opposite sides of the second drive pump 220, and the fourth connecting point is located between the second drive pump 220 and the centrifuge container 110. It is understood that the second connecting point and the third connecting point can be the same connecting point or different connecting points. The connection method between the second connecting conduit 150 and the second tubing assembly 130 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The second connecting conduit 150 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby allowing the liquid flow path to be selected according to the actual situation.

[0033] like Figure 2As shown, in one embodiment, the cell treatment consumable tubing assembly further includes a third connecting tubing 160; one end of the third connecting tubing 160 is connected to the first tubing assembly 120 and located at a fifth connecting point, and the other end of the second connecting tubing 150 is connected to the second tubing assembly 130 and located at a sixth connecting point; the fifth connecting point is located between the first drive pump 210 and the centrifuge container 110, and the sixth connecting point is located between the second drive pump 220 and the centrifuge container 110. It is understood that the sixth connecting point and the fourth connecting point can be the same connecting point or different connecting points. The connection method between the third connecting tubing 160 and the first tubing assembly 120 and the second tubing assembly 130 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The third connecting tubing 160 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby allowing the liquid flow path to be selected according to the actual situation.

[0034] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a sampling tubing 170 for sampling; one end of the sampling tubing 170 is connected to the second tubing assembly 130 and located at a seventh connection point; the seventh connection point is located between the second drive pump 220 and the centrifuge container 110. It is understood that the connection method between the sampling tubing 170 and the second tubing assembly 130 includes, but is not limited to, one or more of a one-piece connection or a multi-port connector connection. A sampling interface may be provided at the end of the sampling tubing 170 away from the second tubing assembly 130 to connect a sampling device, allowing sampling in the centrifuge container 110 through the sampling tubing 170 and the second tubing assembly 130. The sampling tubing 170 may be equipped with, for example... Figure 2 The clip 500 shown opens when sampling is needed and closes when sampling is not needed, preventing liquid from flowing out of the sampling line 170.

[0035] like Figure 2 As shown, in one embodiment, the cell treatment consumable tubing assembly further includes a peripheral device tubing 180 for connecting to peripheral devices; one end of the peripheral device tubing 180 is connected to the first tubing assembly 120 and located at an eighth connection point; the eighth connection point is located between the first drive pump 210 and the centrifuge container 110. It is understood that the connection method between the peripheral device tubing 180 and the first tubing assembly 120 includes, but is not limited to, one or more of a one-piece connection or a multi-port connector connection. An interface for connecting peripheral devices may be provided at the end of the peripheral device tubing 180 away from the first tubing assembly 120. The peripheral device tubing 180 may be provided with, for example... Figure 2The one-way valve 800 shown prevents liquid from flowing out of the peripheral equipment line 180.

[0036] like Figure 2 As shown, in one embodiment, the first tubing assembly 120 includes a first centrifugal line 121 for matching the first drive pump 210, and a first process line 122 for connecting to a first process solution container; one end of the first centrifugal line 121 is connected to the centrifugal container 110, and the other end of the first centrifugal line 121 is connected to the end of the first process line 122 away from the first process solution container. It is understood that the connection point between the first centrifugal line 121 and the first process line 122 can be the same as the first connection point, or it can be a different connection point. The connection method between the first centrifugal line 121 and the first process line 122 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. There can be one or more first process lines 122, thereby connecting different containers. In one embodiment, the first process solution container includes, but is not limited to, a cleaning solution container or an antibody container; the first process line 122 includes, but is not limited to, a cleaning line for connecting to a cleaning solution container or an antibody line for connecting to an antibody container. The first centrifugal line 121 and the first process line 122 can be provided with, for example... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing the selection of the required process solution based on actual conditions.

[0037] As shown in Figure 2, in one embodiment, the first pipe assembly 120 further includes a first transfer pipe 124 for connecting to a first transfer liquid container 123; one end of the first transfer pipe 124, away from the first transfer liquid container 123, is connected to the first process pipe 122. It is understood that the connection between the first transfer pipe 124 and the first process pipe 122 can be, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. There can be one or more first transfer pipes 124, thereby allowing connection to one or more first transfer liquid containers 123 as needed. Figure 2 As shown, the first transfer pipeline 124 can share a switch valve with the first process pipeline 122. At the same time, pipe clamps 400 are respectively installed on the first transfer pipeline 124 and the first process pipeline 122, so that the flow path of the liquid can be selected according to the actual situation.

[0038] like Figure 2As shown, in one embodiment, the second tubing assembly 130 includes a second centrifugal line 131 for matching the second drive pump 220, and a second process line 132 for connecting to a second process solution container; one end of the second centrifugal line 131 is connected to the centrifuge container 110, and the other end of the second centrifugal line 131 is connected to the end of the second process line 132 away from the second process solution container. It is understood that the connection point between the second centrifugal line 131 and the second process line 132 can be the same connection point or a different connection point. The connection method between the second centrifugal line 131 and the second process line 132 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. There can be one or more second process lines 132, thereby connecting different containers. In one embodiment, the second process solution container includes, but is not limited to, a culture medium container, a separation solution container, a cryopreservation solution container, or a virus sample container; the second process tubing 132 includes, but is not limited to, a culture medium tubing for connecting to the culture medium container, a separation solution tubing for connecting to the separation solution container, a cryopreservation solution tubing for connecting to the cryopreservation solution container, or a virus sample tubing 133 for connecting to the virus sample container. The second centrifuge tubing 131 and the second process tubing 132 may be equipped with, for example... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing the selection of the required process solution based on actual conditions.

[0039] like Figure 2 As shown, in one embodiment, the second tube assembly 130 further includes a sample tube 133 for connecting to a sample container; the end of the second centrifuge tube 131 away from the centrifuge container 110 is connected to the end of the sample tube 133 away from the sample container. It is understood that the connection point between the second centrifuge tube 131 and the sample tube 133 can be the same connection point or a different connection point. The connection method between the second centrifuge tube 131 and the sample tube 133 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The sample tube 133 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing selection of whether or not sample solution needs to be introduced based on actual conditions.

[0040] like Figure 2As shown, in one embodiment, the second pipe assembly 130 further includes a waste liquid pipe 135 for connecting to the waste liquid container 134; the end of the second centrifugal pipe 131 away from the centrifugal container 110 is connected to the end of the waste liquid pipe 135 away from the waste liquid container 134. It is understood that the connection point between the second centrifugal pipe 131 and the waste liquid pipe 135 can be the same connection point or a different connection point. The connection method between the second centrifugal pipe 131 and the waste liquid pipe 135 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The waste liquid pipe 135 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that waste liquid can be output according to the actual situation.

[0041] like Figure 2 As shown, in one embodiment, the second pipe assembly 130 further includes a harvesting pipe 136 for connecting to a harvesting container, and the end of the second centrifugal pipe 131 away from the centrifugal container 110 is connected to the end of the harvesting pipe 136 away from the harvesting container. It is understood that the connection point between the second centrifugal pipe 131 and the harvesting pipe 136 can be the same connection point as the second connection point, or it can be a different connection point. The connection method between the second centrifugal pipe 131 and the harvesting pipe 136 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The harvesting pipe 136 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing selection of whether a final product needs to be output based on actual conditions.

[0042] like Figure 2 As shown, in one embodiment, the second tubing assembly 130 further includes a second transfer line 138 for connecting to the second transfer liquid container 137; one end of the second transfer line 138, away from the second transfer liquid container 137, is connected to the second process line 132. It is understood that the connection between the second transfer line 138 and the second process line 132 can be, but is not limited to, one or more of the following: integral connection or connection via a multi-port connector. There can be one or more second transfer lines 138, thereby allowing connection to one or more second transfer liquid containers 137 as needed. Figure 2 As shown, the second transfer pipeline 138 can share a switch valve with the second process pipeline 132. At the same time, pipe clamps 400 are respectively installed on the second transfer pipeline 138 and the second process pipeline 132, so that the flow path of the liquid can be selected according to the actual situation.

[0043] The cell processing consumable assembly of this invention allows for the initial separation of PBMCs from the sample solution in centrifuge container 110. Then, antibodies and viral solutions are added to the PBMCs in centrifuge container 110 for transduction. Next, culture medium is introduced into centrifuge container 110, where the transduced PBMCs are incubated to produce antibody-containing cells. Finally, the antibody-containing cells are harvested into a harvesting container. In this cell processing procedure, multiple steps can be completed sequentially within the consumable assembly, eliminating the need to change equipment after each step to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency.

[0044] like Figure 2 As shown, one embodiment of the present invention also provides a cell processing device, including a first drive pump 210, a second drive pump 220, at least one first switching valve 310 for controlling the on / off state of the pipeline in the first tube group 120, at least one second switching valve 320 for controlling the on / off state of the pipeline in the second tube group 130, a third switching valve 330 for controlling the on / off state of the first connecting pipeline 140, and the cell processing consumable tube group. The first tubing assembly 120 is mounted on the first drive pump 210, which is located between the first connection point and the centrifuge container 110. The second tubing assembly 130 is mounted on the second drive pump 220, which is located between the second connection point and the centrifuge container 110. The first tubing assembly 120 is mounted on the first switching valve 310, which and the centrifuge container 110 are located on opposite sides of the first drive pump 210. The second tubing assembly 130 is mounted on the second switching valve 320, which and the centrifuge container 110 are located on opposite sides of the second drive pump 220. The first connecting pipe 140 is mounted on the third switching valve 330. Understandably, the first drive pump 210 is used to drive the liquid flow in the first tubing assembly 120. The first drive pump 210 includes, but is not limited to, a peristaltic pump, which can drive the liquid flow without contacting the liquid, thus preventing contamination. The second drive pump 220 is used to drive the liquid flow in the second tubing assembly 130. The second drive pump 220 includes, but is not limited to, a peristaltic pump, which can drive the liquid flow without contacting the liquid, thus preventing contamination of the liquid. The first drive pump 210 or the second drive pump 220 can also be other types of liquid pumps, as long as they can achieve the effect of driving liquid flow.

[0045] The cell processing device provided by this invention connects a first tube group 120 and a second tube group 130 to a centrifuge container 110, and establishes a connecting pipe between the first tube group 120 and the second tube group 130. This allows for selective input of solutions connected to the first tube group 120 into the centrifuge container 110 via the second tube group 130, solutions connected to the second tube group 130 into the centrifuge container 110 via the first tube group 120, circulation of the solution within the cell processing consumable tube group, or input of liquid from different positions within the centrifuge container 110 into different containers connected to the first tube group 120 or the second tube group 130. Thus, multiple processes can be sequentially completed within the cell processing consumable tube group during cell processing, eliminating the need to change equipment after each process to proceed to the next, avoiding cell contamination, and improving cell processing efficiency.

[0046] The above are merely embodiments of the cell processing consumable tubing and cell processing equipment of this utility model, and are not intended to limit this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A cell treatment consumable assembly, characterized in that, It includes a centrifuge container, a first tubing assembly connected to the centrifuge container and used for matching a first drive pump, a second tubing assembly connected to the centrifuge container and used for matching a second drive pump, and a first connecting pipeline; One end of the first connecting pipe is connected to the first pipe group and located at the first connection point, and the other end of the first connecting pipe is connected to the second pipe group and located at the second connection point; the first connection point and the centrifuge container are located on opposite sides of the first driving pump, and the second connection point and the centrifuge container are located on opposite sides of the second driving pump.

2. The cell treatment consumable tubing assembly according to claim 1, characterized in that, The cell processing consumable tubing assembly further includes a second connecting tubing; one end of the second connecting tubing is connected to the second tubing assembly and located at a third connecting point, and the other end of the second connecting tubing is connected to the second tubing assembly and located at a fourth connecting point; the third connecting point and the centrifuge container are located on opposite sides of the second drive pump, and the fourth connecting point is located between the second drive pump and the centrifuge container.

3. The cell treatment consumable tubing assembly according to claim 2, characterized in that, The cell processing consumable tubing assembly further includes a third connecting tubing; one end of the third connecting tubing is connected to the first tubing assembly and located at the fifth connecting point, and the other end of the second connecting tubing is connected to the second tubing assembly and located at the sixth connecting point; the fifth connecting point is located between the first drive pump and the centrifuge container, and the sixth connecting point is located between the second drive pump and the centrifuge container.

4. The cell treatment consumable tubing assembly according to claim 1, characterized in that, The cell processing consumable tubing set also includes a sampling tubing for sampling; one end of the sampling tubing is connected to the second tubing set and is located at the seventh connection point; the seventh connection point is located between the second drive pump and the centrifuge container.

5. The cell treatment consumable tubing assembly according to claim 1, characterized in that, The cell processing consumable tubing assembly also includes peripheral equipment tubing for connecting peripheral devices; one end of the peripheral equipment tubing is connected to the first tubing assembly and located at the eighth connection point; the eighth connection point is located between the first drive pump and the centrifuge container.

6. The cell treatment consumable assembly according to claim 1, characterized in that, The first tubing assembly includes a first centrifugal line for matching the first drive pump and a first process line for connecting to a first process solution container; one end of the first centrifugal line is connected to the centrifugal container, and the other end of the first centrifugal line is connected to the end of the first process line away from the first process solution container.

7. The cell treatment consumable assembly according to claim 6, characterized in that, The first pipe assembly further includes a first transfer line for connecting to the first transfer liquid container; the end of the first transfer line away from the first transfer liquid container is connected to the first process line.

8. The cell treatment consumable tubing assembly according to claim 1, characterized in that, The second tubing assembly includes a second centrifugal line for matching the second drive pump and a second process line for connecting to a second process solution container; one end of the second centrifugal line is connected to the centrifugal container, and the other end of the second centrifugal line is connected to the end of the second process line away from the second process solution container.

9. The cell treatment consumable assembly according to claim 8, characterized in that, The second tubing set further includes a sample tubing for connecting to a sample container; one end of the second centrifuge tubing away from the centrifuge container is connected to the other end of the sample tubing away from the sample container; and / or The second tubing assembly further includes a waste liquid line for connecting to a waste liquid container; one end of the second centrifuge line away from the centrifuge container is connected to the end of the waste liquid line away from the waste liquid container; and / or The second pipe assembly further includes a harvesting pipe for connecting to a harvesting container, wherein the end of the second centrifugal pipe away from the centrifugal container is connected to the end of the harvesting pipe away from the harvesting container; and / or The second tubing assembly also includes a second transfer line for connecting to the second transfer liquid container; the end of the second transfer line away from the second transfer liquid container is connected to the second process line.

10. A cell processing device, characterized in that, The assembly includes a first drive pump, a second drive pump, at least one first switching valve for controlling the on / off state of the tubing in the first tubing assembly, at least one second switching valve for controlling the on / off state of the tubing in the second tubing assembly, a third switching valve for controlling the on / off state of the first connecting tubing, and a cell treatment consumable tubing assembly as described in any one of claims 1 to 9. The first tube assembly is mounted on the first drive pump, which is located between the first connection point and the centrifuge container. The second tubing assembly is installed on the second drive pump, which is located between the second connection point and the centrifuge container; the first tubing assembly is installed on the first switch valve, which and the centrifuge container are located on opposite sides of the first drive pump. The second pipe assembly is installed on the second switch valve, and the second switch valve and the centrifuge container are located on opposite sides of the second drive pump; the first connecting pipe is installed on the third switch valve.

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