Closed cell culture device

Abstract

The utility model relates to the field of cell culture, and provides a closed cell culture device. The closed cell culture device comprises a first accommodating body, a second accommodating body, a pump body and a sterile pipeline on-off assembly, wherein the first accommodating body is connected with a first pipeline and is used for cell culture; the second accommodating body is connected with a second pipeline and is used for accommodating a culture medium or cell culture, and the second pipeline is connected with the first pipeline in an on-off manner; the pump body is used for extruding the outer pipe wall of the first pipeline and / or the outer pipe wall of the second pipeline; the sterile pipeline on-off assembly is used for adjusting the first pipeline and the second pipeline to be switched between a pipeline connection state and a pipeline disconnection state; the pump body is used for pumping the culture medium into the first accommodating body from the second accommodating body under the condition that the second accommodating body is used for accommodating the culture medium, and the pump body is used for pumping the cell medium in the first accommodating body into the second accommodating body under the condition that the second accommodating body is used for cell culture. The closed cell culture device is low in environment requirement and low in culture cost.

Description

Technical Field

[0001] This utility model relates to the field of cell culture and provides a closed cell culture device. Background Technology

[0002] Cell therapy, as an emerging medical technology, has great potential to treat a variety of diseases, especially malignant tumors. However, traditional cell therapy production processes rely on open-system operations, which are susceptible to contamination. This places high demands on the environment for open-system operations, and the production scale is limited, making it difficult to meet clinical medical needs.

[0003] To overcome the aforementioned problems, some companies are currently dedicated to developing automated cell culture systems. However, most existing solutions only address partial automation and closed-loop systems. Furthermore, existing automated systems suffer from overly complex layouts, large footprints, and high hardware and manpower costs, hindering the large-scale production of cell therapy. Therefore, reducing the risk of contamination during cell culture while lowering costs has become a key technical challenge for those skilled in the art. Utility Model Content

[0004] This invention provides a closed cell culture device to address the shortcomings of related technologies, such as high risk of contamination, high environmental requirements, and high culture costs during cell culture.

[0005] This utility model embodiment provides a closed cell culture device, comprising:

[0006] A first container is provided with a first conduit, and the first container is used for cell culture.

[0007] The second container is connected to a second conduit, which is connected to the first conduit in a way that allows it to be switched on or off. The second container is used to hold culture media or for cell culture.

[0008] Pump body, the pump body being used to squeeze the outer wall of the first pipeline and / or the outer wall of the second pipeline;

[0009] A sterile tubing connection / disconnection assembly, wherein the sterile tubing connection / disconnection assembly is used to adjust the switching between the first tubing and the second tubing in a tubing connected state and a tubing disconnected state;

[0010] When the second container is used to contain culture medium, the pump is used to pump the culture medium from the second container into the first container; when the second container is used for cell culture, the pump is used to pump the cell medium in the first container into the second container.

[0011] According to one embodiment of the present invention, a detection element is provided in the first container, the detection element is placed below the first container, and the detection element is used to detect the state parameters of the cell medium in the first container;

[0012] The pump body is equipped with a receiver, which is wirelessly connected to the detection element, and the receiver is used to control the working state of the pump body based on the status parameters.

[0013] According to one embodiment of the present invention, a control element is further included, which is wirelessly connected to at least one of the detection element and the driving element.

[0014] According to one embodiment of the present invention, the pump body includes a driving component, which is wirelessly connected to the receiving component.

[0015] According to one embodiment of the present invention, a flow meter is provided on the pump body. When the pipeline is in the connected state, the flow meter is used to detect the flow rate of the medium flowing through the first pipeline and / or the second pipeline. The flow meter is wirelessly connected to the control component.

[0016] According to one embodiment of the present invention, the aseptic tubing connection / disconnection assembly includes:

[0017] A connecting fitting, the connecting fitting being used to adjust the first pipeline and the second pipeline to switch from the pipeline disconnected state to the pipeline connected state;

[0018] A heat-sealing component is used to adjust the first pipeline and the second pipeline to switch from the pipeline connection state to the pipeline disconnection state.

[0019] According to one embodiment of the present invention, the first accommodating body includes:

[0020] A receiving body having a receiving cavity formed therein for receiving the cell medium;

[0021] A cover is movably mounted on the receiving body, and the cover has a mounting hole, through which the first pipeline is installed.

[0022] According to one embodiment of the present invention, the cover is further provided with a vent hole, the vent hole is provided with a filter, the vent hole is in fluid communication with the accommodating cavity, and a vent pipe is installed in the vent hole.

[0023] According to one embodiment of the present invention, it further includes an incubator, wherein a plurality of culture tanks are provided in the incubator, and the first accommodating body is movably installed in the culture tanks.

[0024] According to one embodiment of the present invention, it further includes a first centrifuge element and a second centrifuge element. The first centrifuge element is disposed upstream of the incubator, and the second centrifuge element is disposed downstream of the incubator. The first centrifuge element is used to separate initial cells in the blood sample, and the second centrifuge element is used to separate target cells in the cell medium.

[0025] According to the closed cell culture device provided in this embodiment, the second container is designed to function as both a storage container for the culture medium and another cell culture site, greatly improving the flexibility of cell culture. Users can easily switch the function of the second container according to experimental needs. By introducing a pump and a sterile tubing switching component, the device automates medium transfer and cell culture. This not only improves operational convenience but also reduces errors and contamination risks caused by human intervention. This allows cell culture to be performed without being limited to the harsh culture environments of related technologies, thereby reducing the environmental requirements for cell culture in related technologies and lowering the cost of cell culture. Moreover, the automated design and flexible function switching allow users to perform cell culture experiments more efficiently while ensuring the accuracy and consistency of experimental results. Thus, the closed cell culture device of this invention, through innovative design and ingenious functional integration, provides a highly efficient, flexible, simple, highly automated, intelligently integrated, high-efficiency, and low-cost solution for cell culture experiments. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0027] Figure 1 This is a schematic diagram of the closed cell culture device provided in this embodiment of the utility model.

[0028] Figure 2 This is a schematic enlarged view of the first accommodating body provided in an embodiment of the present utility model.

[0029] Figure label:

[0030] 100. First container; 102. First pipeline; 104. Second container; 106. Second pipeline; 108. Pump body; 110. Control unit; 112. Container body; 114. Cover; 116. Ventilation pipeline; 118. Incubator; 120. Culture tank; 122. First centrifuge unit; 124. Second centrifuge unit. Detailed Implementation

[0031] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0032] like Figures 1 to 2 As shown, this embodiment of the present invention provides a closed cell culture device, comprising:

[0033] A first container 100 is connected to a first conduit 102, and the first container 100 is used for cell culture.

[0034] The second container 104 is connected to a second conduit 106, which is connected to the first conduit 102 in a shunt manner. The second container 104 is used to contain culture medium or for cell culture.

[0035] Pump body 108 is used to squeeze the outer wall of the first pipe 102 and / or the outer wall of the second pipe 106.

[0036] The aseptic tubing on / off assembly is used to adjust the switching between the first tubing 102 and the second tubing 106 in a tubing connected state and a tubing disconnected state.

[0037] When the second container 104 is used to contain culture medium, the pump 108 is used to pump the culture medium from the second container 104 into the first container 100. When the second container 104 is used for cell culture, the pump 108 is used to pump the cell medium in the first container 100 into the second container 104.

[0038] According to the closed cell culture device provided in this embodiment, the second container 104 is designed to function as both a storage container for the culture medium and another cell culture site, greatly improving the flexibility of cell culture. Users can easily switch the function of the second container 104 according to experimental needs. By introducing a pump body 108 and a sterile tubing switching component, the device automates medium transfer and cell culture. This not only improves operational convenience but also reduces errors and contamination risks caused by human intervention. This allows cell culture to be performed without being limited to the harsh culture environments of related technologies, thereby reducing the environmental requirements for cell culture in related technologies and lowering the cost of cell culture. Moreover, the automated design and flexible function switching allow users to perform cell culture experiments more efficiently while ensuring the accuracy and consistency of experimental results. Thus, the closed cell culture device of this invention, through innovative design and ingenious functional integration, provides a highly efficient, flexible, simple, highly automated, intelligently integrated, high-efficiency, and low-cost solution for cell culture experiments.

[0039] Please continue reading Figures 1 to 2 The closed cell culture device of this utility model mainly includes a first container 100, a second container 104, a pump body 108, and a sterile pipeline on / off assembly.

[0040] The first container 100 is the core component, designed for cell culture. It is connected to a first conduit 102, which serves as the inlet and outlet channel for the culture medium and cell culture medium. Furthermore, it should be noted that there can be two first conduits 102, one for pumping in the culture medium and the other for pumping out the cell culture medium.

[0041] The second container 104 has multiple functions, serving both as a container for culture media (such as nutrient solutions and growth factors) and as another site for cell culture. It is connected to a second conduit 106, which is structurally connected to the first conduit 102 in a way that allows for switching on and off. Furthermore, it should be noted that there can be two second conduits 106, one for pumping in the culture media and the other for pumping out the cell culture medium.

[0042] In this embodiment of the utility model, there may be two first pipes 102 and one second pipe 106, or one first pipe 102 and two second pipes 106, or both the first pipe 102 and the second pipe 106 may be two.

[0043] The pump body 108 is the power source in the entire closed cell culture device. It can generate pressure by squeezing the outer wall of the first pipe 102 or the second pipe 106, thereby realizing the transfer of media or cells.

[0044] It should be noted that the pump body 108 can be a peristaltic pump or other components that do not come into direct contact with the medium.

[0045] The sterile tubing on / off assembly is a control component used to adjust the connection state between the first tubing 102 and the second tubing 106 when needed, that is, to switch between the tubing connected state and the tubing disconnected state, while ensuring a sealed and uncontaminated environment.

[0046] When using this closed cell culture device, the operating procedure will vary depending on the intended use of the second container 104:

[0047] When the second container 104 is used to contain culture medium, the pump 108 is activated to pump the culture medium from the second container 104 through the second pipeline 106, through the sterile pipeline switching component (which is in a connected state), and then through the first pipeline 102 into the first container 100 for cell culture.

[0048] When the second container 104 is used for cell culture, the operation process is reversed. The pump 108 is used to pump the cell medium (including proliferated cells and culture medium with increased volume) in the first container 100 through the first tubing 102, the sterile tubing switch assembly (also in a connected state), then into the second tubing 106, and finally into the second container 104 to achieve further cell culture and expansion.

[0049] According to one embodiment of the present invention, a detection element is provided in the first container 100, and the detection element is placed below the first container 100. The detection element is used to detect the state parameters of the cell medium in the first container 100. A receiver is provided on the pump body 108, and the receiver is wirelessly connected to the detection element. The receiver is used to control the working state of the pump body 108 based on the state parameters.

[0050] In one embodiment of this invention, the detection element is placed below the first container. This design allows the detection element to directly or indirectly acquire the state parameters of the cell media in the first container 100. These state parameters include, but are not limited to, image information. The detection element uses high-precision sensor technology to convert these state parameters into electrical signals in real time or periodically for subsequent analysis and processing.

[0051] The main function of the detection device is to monitor the state parameters of the cell media in the first container 100 in real time. These state parameters may include, but are not limited to, image information.

[0052] Controllers such as microprocessors or controllers 110 are responsible for receiving status parameter signals from the detectors and automatically analyzing and formulating processing rules for the cell medium in the first container based on these signals and predetermined processing standards. These processing rules may involve adjusting the composition of the culture medium, replacing the culture medium, adjusting the culture environment, etc.

[0053] The receiver is integrated into the pump body, offering high reliability and rapid response. It connects wirelessly to the controller 110, enabling it to receive operational commands from the controller in real time. These commands may involve various aspects such as pump start-up, shutdown, and delivery rate adjustment, aiming to ensure that the cell media is processed in a predetermined manner.

[0054] By directly mounting the receiver on the pump body and wirelessly connecting it to the controller, the path and time for command transmission are significantly shortened, thereby improving the system's response speed. This allows the system to more quickly adjust the pump's operating state to adapt to various changes during cell culture. As a direct communication interface between the pump and controller, the receiver ensures accurate transmission of operating commands to the pump. This avoids potential misunderstandings or distortions during command transmission, thus improving the accuracy of command execution. Integrating the receiver onto the pump body reduces the need for additional communication modules or interfaces, simplifying the overall system design. This not only reduces system complexity and cost but also improves system reliability and stability. Because the receiver can receive and respond to operating commands from the controller in real time, the system can flexibly adjust the pump's operating state according to actual needs. This allows the system to adapt to various cell culture scenarios and requirements, improving its flexibility and adaptability.

[0055] According to one embodiment of the present invention, the pump body 108 includes a drive component, and the receiver is wirelessly connected to the drive component.

[0056] In one embodiment of the present invention, the pump body 108 is a key component in the cell culture or processing system, responsible for pumping or extracting cell media into the first container 100.

[0057] In this embodiment, the pump body 108 includes not only the previously mentioned receiving component but also a driving component. The driving component is the power source for the pump body 108, responsible for providing the energy required for the pump body 108 to operate. The driving component can be a motor or other device capable of providing stable power output.

[0058] A wireless connection is established between the receiver and the drive unit. This means that the receiver can receive status parameter data from the detection unit and send control signals to the drive unit based on this data. The drive unit adjusts its operating state according to the received control signals, thereby achieving precise control of the operating state of the pump body 108.

[0059] Because a wireless connection is established between the receiver and the actuator, the system can respond more quickly to changes in state parameters detected by the detector. This means that when the state parameters of the cell medium change, the system can adjust the operating state of the pump 108 more quickly, thereby maintaining the cell medium in an optimal environment. Precise control of the actuator's operating state ensures more stable output from the pump 108. This helps maintain the stability of the cell medium's state, thereby improving the efficiency and quality of cell culture or processing. Direct wireless connection between the receiver and the actuator eliminates the need for some intermediate transmission devices or control mechanisms. This simplifies the system structure and reduces system complexity and maintenance costs. Since the receiver can flexibly adjust the actuator's operating state based on data transmitted by the detector, this technology can adapt to different types of cells and different culture or processing needs.

[0060] According to one embodiment of the present invention, a control element 110 is also included, which is wirelessly connected to the detection element.

[0061] In one embodiment of this utility model, in addition to the pump body 108 including a drive component, a receiver component and a drive component wirelessly connected to the drive component, a control component 110 is added, and the control component 110 is wirelessly connected to the detection component.

[0062] The control unit 110 is a key component in the closed cell culture device. It is responsible for receiving status parameter data from the detection unit and analyzing and processing this data according to preset logic or algorithms. The control unit 110 can also send control signals to the drive unit based on the analysis results to adjust the operating state of the pump body 108.

[0063] A wireless connection is established between the control unit 110 and the detection unit, meaning that the control unit 110 can receive status parameter data from the detection unit in real time, especially cell culture status image information. This data includes, but is not limited to, image information.

[0064] By introducing the control unit 110, the closed cell culture device achieves intelligent analysis and processing of state parameters. The control unit 110 can precisely control the pump 108 based on accurate data transmitted from the detection unit. This helps maintain the stability of the cell medium state, improving the efficiency and quality of cell culture or processing. As the central hub of the system, the control unit 110 can monitor and process data from the detection unit in real time. If an abnormal state is detected, the control unit 110 can quickly take measures, such as stopping the pump 108 or issuing an alarm, to protect the cells and the culture environment. Intelligent and automated control simplifies user operation. Users can monitor and control the status of the closed cell culture device through an interface or remotely, reducing operational difficulty and cost.

[0065] In addition, the pump body 108 is also controlled by the control unit 110 and can non-invasively feed back information on the liquid flow rate and volume to the control unit 110.

[0066] According to one embodiment of the present invention, a flow meter is provided on the pump body 108. When the pipeline is connected, the flow meter is used to detect the flow rate of the medium in the first pipeline 102 and / or the second pipeline 106. The flow meter is wirelessly connected to the control unit.

[0067] In one embodiment of the present invention, a flow meter is a key component on the pump body 108, used to detect the flow rate of the medium in the first pipeline 102 and / or the second pipeline 106 when the pipelines are connected.

[0068] The first pipeline 102 can be connected to the first container 100 for pumping or extracting media therein; the second pipeline 106 is connected to other processing units or storage containers for the circulation, recovery or discharge of media.

[0069] The pipeline connectivity state refers to the state in which the medium can flow freely between the pump body 108, the first pipeline 102, the second pipeline 106, and other components connected to them (such as the first container 100). In this state, the flow meter can accurately measure the flow rate of the medium in the pipeline.

[0070] A wireless connection is established between the flow meter and the control unit, meaning that the flow meter can transmit the detected medium flow data to the control unit in real time. The control unit can adjust the pump's operating state based on the received flow data, thereby achieving precise control of the pump's output flow.

[0071] By introducing a flow meter and wirelessly connecting it to the control unit, the system can monitor and control the flow rate of the medium in the pipeline in real time. This helps improve the accuracy of flow control, ensuring that the cellular medium is circulated or processed at an appropriate rate. Precise flow control helps avoid media waste or insufficiency. The system can adjust the output flow rate of pump 108 according to actual needs, thereby optimizing the utilization efficiency of the medium. The real-time monitoring function of the flow meter can help the system detect potential flow anomalies or faults in a timely manner. Once an anomaly is detected, the system can take corresponding measures to adjust or alarm, thereby enhancing the stability and reliability of the system. Users can monitor and control the flow meter readings and the operating status of pump 108 through an interface or remotely. This allows users to have a more intuitive understanding of the system's operating status and make adjustments or optimizations as needed.

[0072] According to one embodiment of the present invention, the aseptic tubing connection / disconnection component includes:

[0073] Connecting fitting, used to switch the first pipeline 102 and the second pipeline 106 from a disconnected state to a connected state;

[0074] The heat-sealing component is used to switch the first pipeline 102 and the second pipeline 106 from a pipeline connected state to a pipeline disconnected state.

[0075] In one embodiment of this invention, the connecting fitting is a key component used to adjust the connection state between the first pipe 102 and the second pipe 106 when needed, switching from a disconnected state to a connected state. The connecting fitting allows users or the system to automatically or manually connect the two pipes, enabling the medium to flow freely between them.

[0076] The heat seal is used to adjust the connection between the first conduit 102 and the second conduit 106 when needed, but to switch from a connected state to a disconnected state. The heat seal typically achieves permanent closure or disconnection of the conduit by heating a portion of it to melt or fuse the conduit material. This method is often used in single-use systems to avoid cross-contamination or reuse.

[0077] In the initial state, the first pipe 102 and the second pipe 106 are disconnected to ensure the safety and cleanliness of the system. When it is necessary for the medium to flow between the two pipes, they can be connected using a fitting to achieve pipe connectivity. If it is necessary to permanently disconnect the connection between the two pipes, a heat-sealing fitting can be used to heat-seal the pipes.

[0078] By combining fittings and heat-sealing devices, the system allows for flexible adjustment of the connections between pipes as needed. This enables the system to adapt to different operational requirements, such as media circulation, recovery, discharge, or isolation. Heat-sealing devices provide a way to permanently seal the piping, helping to prevent cross-contamination or accidental leaks. This is particularly important for cell culture or processing systems requiring high cleanliness or sterile conditions. The use of fittings and heat-sealing devices helps to precisely control the flow path and volume of the media. This helps optimize media utilization efficiency, reduce waste, and ensure that cells grow and are processed under optimal conditions. Although the heat-sealing process may require specialized equipment or tools, once completed, it provides a simple and reliable method to disconnect the piping connections. The use of fittings is also generally relatively simple, allowing for quick connection or disconnection of piping, either manually or automatically.

[0079] According to one embodiment of the present invention, the first accommodating body 100 includes:

[0080] The accommodating body 112 has an accommodating cavity formed therein for accommodating cell media;

[0081] The cover 114 is movably installed on the housing body 112. The cover 114 has an installation hole, and the first pipe 102 is installed in the installation hole.

[0082] In one embodiment of this invention, the accommodating body 112 is the main body of the first accommodating body 100, and an accommodating cavity for accommodating cell media is formed inside it. The size and shape of the accommodating cavity need to be designed according to the specific needs of cell culture or processing to ensure that the cell media can be uniformly distributed therein and provide a suitable growth environment. The accommodating body 112 may be made of transparent or translucent material to facilitate observation of the state of the cell media and the growth of cells.

[0083] The cover 114 is a component movably mounted on the receiving body 112 for sealing the opening of the receiving cavity. The cover 114 has one or more mounting holes for mounting the first conduit 102 or other necessary connections. The design and material selection of the cover 114 should ensure that it can tightly seal the receiving cavity, preventing cell media leakage or the entry of external contaminants.

[0084] The first conduit 102 is connected to the accommodating cavity through the mounting hole on the cover 114, and is used to pump or extract cell media into the accommodating cavity.

[0085] During cell culture or processing, the cell medium is placed in the containment cavity and circulated, replaced, or replenished via the first tubing 102. The movable mounting design of the cover 114 allows the user or system to automatically or manually open and close the containment cavity for cell manipulation, sampling, or maintenance. The mounting hole design allows the first tubing 102 to be easily connected to the containment cavity while maintaining the overall seal and cleanliness of the system.

[0086] The movable mounting design of the cover 114 allows users to easily open and close the container cavity, facilitating cell manipulation and maintenance. The mounting hole design makes connecting and disconnecting the first tubing 102 simple and quick. The tight fit between the cover 114 and the container body 112, along with the sealing design of the mounting hole, helps prevent cell medium leakage or external contaminants from entering the container cavity. This is crucial for maintaining the cleanliness and stability of the cell culture or processing environment. The size and shape design of the container cavity, as well as the selection of transparent or translucent materials, contribute to providing a suitable cell growth environment. Users can monitor the state of the cell medium and cell growth by observing the inside of the container cavity, allowing for timely adjustments to culture conditions. The design of the first tubing 102 connecting to the container cavity via the mounting hole allows the system to flexibly adjust the medium flow path and operating mode as needed. This helps meet different cell culture or processing requirements.

[0087] According to one embodiment of the present invention, a vent hole is also provided on the cover 114, which is in fluid communication with the accommodating cavity, and a vent pipe 116 is installed in the vent hole.

[0088] In one embodiment of this invention, the vent is one or more small holes formed on the cover 114 to allow fluid communication between the accommodating cavity and the external environment. The design of the vent should ensure that it meets the needs of gas exchange while preventing external pollutants or microorganisms from entering the accommodating cavity.

[0089] Vent line 116 is a tubular assembly installed in a vent hole for guiding and regulating the flow of gas within the vent hole. Vent line 116 may include filters, valves, or other connecting devices to further control the entry and exit of gas and prevent contamination.

[0090] During cell culture or processing, cell metabolism produces carbon dioxide and other gases, while consuming oxygen. The combined design of the vents and ventilation channels 116 allows these gases to exchange between the containment chamber and the external environment, thereby maintaining a suitable gaseous environment for cell growth. Additional devices such as filters can further purify the gases entering the containment chamber, preventing the intrusion of external contaminants or microorganisms.

[0091] The combined design of the vent and venting line 116 enables the cell culture or processing system to maintain a suitable gaseous environment, including appropriate oxygen and carbon dioxide concentrations. This helps promote healthy cell growth and metabolic activity. By installing additional devices such as filters, the vent and venting line 116 can effectively prevent external contaminants or microorganisms from entering the containment chamber. This helps reduce the risk of contamination during cell culture or processing and improves the overall safety of the system. The venting line 116 may include valves or other connecting devices, allowing users to finely control gas exchange as needed. This enables the system to adapt to different cell culture or processing requirements, improving its flexibility and adaptability. The design of the vent and venting line 116 simplifies and facilitates the management of the gas exchange process. Users can monitor and control the gas environment by observing the status of the venting line 116 or adjusting relevant parameters, thereby simplifying the operation process and improving work efficiency.

[0092] According to one embodiment of the present invention, it further includes an incubator 118, in which a plurality of culture tanks 120 are provided, and a first accommodating body 100 is movably installed in the culture tank 120.

[0093] In one embodiment of this invention, the incubator 118 is a closed or semi-closed device used to provide constant environmental conditions, such as temperature, humidity, and light, required for cell culture or processing. The incubator 118 may be equipped with heating, cooling, humidification, and dehumidification systems to ensure that cells grow under optimal conditions.

[0094] A culture tank 120 is a container or area inside an incubator 118 for placing and culturing cells. In this embodiment, multiple culture tanks 120 are provided in the incubator 118 to simultaneously culture multiple cell samples or perform different cell treatment experiments. The size and shape of the culture tank 120 need to be designed according to the specific needs of cell culture or treatment.

[0095] The first container 100 can be movably installed in the culture tank 120. This means that the user can remove or insert the first container 100 from the culture tank 120 as needed to facilitate cell manipulation, sampling, media replacement, or maintenance. The movable installation design may include slide rails, snap-fit ​​mechanisms, locking devices, etc., to ensure the stability and ease of operation of the first container 100 in the culture tank 120.

[0096] During cell culture or processing, the first container 100 is placed in the culture tank 120, and the cell medium is placed in the container cavity, with gas exchange occurring through vents and ventilation lines 116. The incubator 118 provides constant environmental conditions to ensure that cells grow and are processed under optimal conditions. Users can remove or place the first container 100 from the culture tank 120 at any time according to experimental needs, performing necessary operations and maintenance.

[0097] By setting up multiple culture tanks 120, the incubator 118 can simultaneously culture multiple cell samples or conduct different cell treatment experiments, thereby improving culture efficiency. The constant environmental conditions provided by the incubator 118 help maintain the appropriate temperature, humidity, and light required for cell growth, thus optimizing the culture environment. The movable installation design of the first container 100 allows users to easily remove or place it from the culture tank 120, facilitating cell manipulation, sampling, or maintenance. The multiple culture tanks 120 and the movable first container 100 enable the system to adapt to different cell culture or treatment needs, improving the system's flexibility and adaptability. The closed or semi-closed design of the incubator 118 and the airtightness of the first container 100 help prevent external contaminants or microorganisms from entering the culture environment, ensuring the safety of the culture.

[0098] According to one embodiment of the present invention, it further includes a first centrifuge element 122 and a second centrifuge element 124. The first centrifuge element 122 is disposed upstream of the incubator 118, and the second centrifuge element 124 is disposed downstream of the incubator 118. The first centrifuge element 122 is used to separate initial cells in the blood sample, and the second centrifuge element 124 is used to separate target cells in the cell medium.

[0099] In one embodiment of the present invention, the first centrifuge element 122 is located upstream of the incubator 118, and its main function is to separate the initial cells from blood samples or other biological samples.

[0100] During centrifugation, the difference in centrifugal force generated by different substances during rotation is used to effectively separate cells from other components (such as plasma, serum, etc.). The separated initial cells can then be transferred to the first container 100 or other suitable containers for further culture or processing.

[0101] The second centrifuge element 124 is positioned downstream of the incubator 118, and its primary function is to separate target cells from the cell culture medium. During cell culture or processing, the cell culture medium may contain multiple cell types, and the second centrifuge element 124 can use centrifugal force to separate these cells in order to obtain specific target cells. The separated target cells can be used for subsequent cell analysis, expansion, treatment, or other applications.

[0102] It should be noted that in this embodiment, "upstream" and "downstream" refer to their relative positions in the cell processing flow. Upstream typically refers to the initial stage of the processing flow, while downstream refers to subsequent stages of the processing flow.

[0103] The first centrifuge element 122, as an upstream component, is responsible for separating the initial cells from the original sample; the second centrifuge element 124, as a downstream component, is responsible for separating the target cells from the cell medium.

[0104] In the cell processing procedure, the original blood sample or other biological sample is first sent to the first centrifuge 122 for centrifugation to obtain the initial cells.

[0105] The initial cells are then transferred to the first container 100 or other container in the incubator 118 for culture, amplification or other treatment.

[0106] The processed cell medium (which may contain multiple cell types) is then sent to a second centrifuge unit 124 for centrifugation to obtain the target cells.

[0107] Ultimately, the isolated target cells can be used for subsequent cell analysis, expansion, treatment, or other applications.

[0108] By configuring the first centrifuge element 122 and the second centrifuge element 124, the system can efficiently separate the initial cells in the blood sample from the target cells in the cell medium. This helps reduce the time and cost in the cell processing process while improving cell purity and quality. The upstream and downstream layout of the first centrifuge element 122 and the second centrifuge element 124 makes the cell processing flow clearer and more orderly. This helps reduce operational complexity and improve the overall efficiency and reliability of the system. The system can flexibly adjust the operating parameters (such as centrifugation speed, time, etc.) of the first centrifuge element 122 and the second centrifuge element 124 according to different cell processing needs. This allows the system to adapt to various cell types and processing conditions, improving the system's adaptability and flexibility. During centrifugation, the effective separation of cells from other components helps reduce the risk of cross-contamination. At the same time, the closed or semi-closed design of the system also helps prevent the intrusion of external contaminants or microorganisms, ensuring the safety of cell processing.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A closed cell culture device, characterized in that, include: A first container (100) is connected to a first conduit (102), and the first container (100) is used for cell culture; The second container (104) is connected to a second conduit (106), which is connected to the first conduit (102) in a shunt manner. The second container (104) is used to contain culture medium or for cell culture. Pump body (108), the pump body (108) is used to squeeze the outer wall of the first pipeline (102) and / or the outer wall of the second pipeline (106); A sterile tubing connection / disconnection assembly is used to adjust the first tubing (102) and the second tubing (106) to switch between a tubing connection state and a tubing disconnection state; When the second container (104) is used to contain culture medium, the pump (108) is used to pump the culture medium from the second container (104) into the first container (100). When the second container (104) is used for cell culture, the pump (108) is used to pump the cell medium in the first container (100) into the second container (104).

2. The closed cell culture device according to claim 1, characterized in that, The first container (100) is provided with a detection element, which is placed below the first container (100) and is used to detect the state parameters of the cell medium in the first container (100). A receiver is provided on the pump body (108), the receiver is wirelessly connected to the detection element, and the receiver is used to control the working state of the pump body (108) based on the state parameters.

3. The closed cell culture device according to claim 2, characterized in that, It also includes a control unit (110) that is wirelessly connected to the detection unit.

4. The closed cell culture device according to claim 3, characterized in that, The pump body (108) includes a drive unit that is wirelessly connected to the receiver.

5. The closed cell culture device according to claim 3, characterized in that, A flow meter is provided on the pump body (108). When the pipeline is connected, the flow meter is used to detect the flow rate of the medium flowing through the first pipeline (102) and / or the second pipeline (106). The flow meter is wirelessly connected to the control unit.

6. The closed cell culture apparatus according to any one of claims 1 to 5, characterized in that, The aseptic tubing connection / disconnection assembly includes: A connecting fitting is used to switch the first pipeline (102) and the second pipeline (106) from a disconnected state to a connected state. A heat-sealing component is used to switch the first pipeline (102) and the second pipeline (106) from the pipeline connected state to the pipeline disconnected state.

7. The closed cell culture apparatus according to any one of claims 1 to 5, characterized in that, The first accommodating body (100) includes: A receiving body (112) having a receiving cavity formed therein for receiving the cell medium; A cover (114) is movably mounted on the receiving body (112), and an installation hole is provided on the cover (114), and the first pipeline (102) is installed in the installation hole.

8. The closed cell culture device according to claim 7, characterized in that, The cover (114) is also provided with a vent hole, the vent hole is equipped with a filter, the vent hole is in fluid communication with the accommodating cavity, and a vent pipe (116) is installed in the vent hole.

9. The closed cell culture apparatus according to any one of claims 1 to 5, characterized in that, It also includes an incubator (118) in which a plurality of culture tanks (120) are provided, and the first accommodating body (100) is movably installed in the culture tank (120).

10. The closed cell culture device according to claim 9, characterized in that, It also includes a first centrifuge element (122) and a second centrifuge element (124). The first centrifuge element (122) is located upstream of the incubator (118), and the second centrifuge element (124) is located downstream of the incubator (118). The first centrifuge element (122) is used to separate initial cells from the blood sample, and the second centrifuge element (124) is used to separate target cells from the cell medium.

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