Special pore plate accessory for magnetic intervention cell three-dimensional culture

Abstract

The utility model relates to the field of medical instruments, and discloses a special pore plate accessory for magnetic intervention of three-dimensional cell culture, which comprises a body, an adsorption mechanism and a limiting mechanism, wherein the adsorption mechanism comprises a top cover arranged at the upper end of the body, and an adsorption assembly is arranged in the top cover; the limiting mechanism comprises a balance part arranged on the lower portion of the top cover, and a limiting assembly is arranged at the lower end of the balance part. According to the pore plate accessory special for the magnetic intervention cell three-dimensional culture, the body combining the adsorption mechanism and the limiting mechanism is adopted, and the adsorption assembly can ensure that magnetic microspheres or magnetic hydrogel is kept in a suspension state in a culture medium and is prevented from settling to the bottom of the pore plate; meanwhile, the limiting assembly can effectively prevent the magnetic microspheres or the magnetic hydrogel from floating to the surface of the culture medium under the action of a magnetic field, so that the cells can be uniformly stressed and fully contacted with the culture medium, and the stability of a three-dimensional culture system is ensured.

Description

Technical Field

[0001] This utility model relates to the field of medical devices, and in particular to a special well plate accessory for magnetic intervention in three-dimensional cell culture. Background Technology

[0002] In research settings for magnetically induced stem cell differentiation into chondrocytes, researchers typically use magnetic microspheres or magnetic hydrogels for three-dimensional culture and apply mechanical force using an external magnetic field to promote cell differentiation. However, some problems exist with existing techniques:

[0003] Existing methods of applying magnetic fields make it difficult to precisely control the position of magnetic microspheres or magnetic hydrogels. Placing a magnet directly below the well plate will cause the magnetic material to adhere tightly to the bottom of the well plate, affecting its three-dimensional suspension state. Placing a magnet above the well plate may cause it to float to the surface of the culture medium, preventing cells from fully contacting the culture medium and affecting their survival rate.

[0004] Therefore, there is an urgent need to design a special well plate accessory for magnetic intervention in three-dimensional cell culture that can solve the above technical problems. Utility Model Content

[0005] In view of the problems mentioned in the background art, the purpose of this utility model is to provide a special well plate accessory for magnetic intervention in three-dimensional cell culture, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model mainly provides the following technical solutions:

[0007] A magnetically-assisted three-dimensional cell culture plate accessory, comprising a main body, and further comprising:

[0008] Adsorption mechanism and confinement mechanism; wherein:

[0009] The adsorption mechanism includes a top cover located at the upper end of the body, and an adsorption component is provided inside the top cover;

[0010] The limiting mechanism includes a balancing component located at the bottom of the top cover, and a limiting component is provided at the lower end of the balancing component.

[0011] Furthermore, the adsorption assembly includes a magnet groove formed inside the center of the top cover, and a magnet sheet is fixedly installed inside the magnet groove.

[0012] Furthermore, the limiting component includes a gas-liquid balance groove extending through the periphery of the balancer, and a barrier net is provided at the lower end of the balancer.

[0013] Furthermore, there are multiple gas-liquid balance tanks, which are evenly distributed around the periphery of the balance component;

[0014] The mesh size of the barrier net is slightly smaller than the particle size of the magnetic microspheres or magnetic hydrogels.

[0015] Furthermore, the body has three specifications: 6-hole plate, 12-hole plate and 24-hole plate. The outer diameter of its top cover is slightly larger than the inner diameter of the corresponding small hole plate, and the outer diameter of the balancing component is slightly smaller than the inner diameter of the corresponding small hole plate.

[0016] Furthermore, the top cover is made of a transparent material that is corrosion-resistant and does not affect magnetic field conduction; the balancing component is made of a transparent material that is biocompatible and has a certain mechanical strength; and the barrier net is made of a material that is highly permeable and resistant to biodegradation.

[0017] Compared with the prior art, the beneficial effects of this utility model are mainly reflected in:

[0018] Compared to existing methods that directly place magnets at the top and bottom of well plates, leading to uncontrolled positioning of magnetic microspheres or hydrogels, this invention addresses this issue by designing a magnetically-assisted well plate accessory for three-dimensional cell culture. The accessory combines an adsorption mechanism and a confinement mechanism. The adsorption component ensures the magnetic microspheres or hydrogels remain suspended in the culture medium, preventing them from settling to the bottom of the well plate. Simultaneously, the confinement component effectively prevents the magnetic microspheres or hydrogels from floating to the surface of the culture medium under the influence of the magnetic field. This ensures that cells are evenly stressed and in full contact with the culture medium, thereby guaranteeing the stability of the three-dimensional culture system.

[0019] The above description is only an overview of the technical solution of this utility model. In order to clearly understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This utility model relates to a three-dimensional well plate accessory for magnetic intervention in three-dimensional cell culture. Figure 1 (12-hole plate specifications);

[0022] Figure 2 This utility model relates to a three-dimensional well plate accessory for magnetic intervention in three-dimensional cell culture. Figure 2 (24-hole plate specifications);

[0023] Figure 3This utility model relates to a three-dimensional well plate accessory for magnetic intervention in three-dimensional cell culture. Figure 3 (6-hole plate specifications);

[0024] Figure 4 This utility model relates to a three-dimensional well plate accessory for magnetic intervention in three-dimensional cell culture. Figure 4 (In conjunction with the usage of perforated plates);

[0025] Figure 5 This is a structural diagram of a special well plate accessory for magnetic intervention in three-dimensional cell culture according to this utility model;

[0026] Figure 6 For the present utility model Figure 5 Enlarged view of section A;

[0027] Explanation of reference numerals in the attached figures:

[0028] 1. Body; 2. Adsorption mechanism; 3. Confinement mechanism; 4. Orifice plate;

[0029] 21. Top cover; 22. Adsorption assembly;

[0030] 31. Balancing component; 32. Limiting component;

[0031] 221. Magnetic groove; 222. Magnetic sheet;

[0032] 321. Gas-liquid balance tank; 322. Barrier net;

[0033] 41. Small room; Detailed Implementation

[0034] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the relevant utility model and not intended to limit the scope of the utility model. Furthermore, it should be noted that, for ease of description, only the parts relevant to the utility model are shown in the accompanying drawings.

[0035] like Figure 1-6 As shown, this utility model discloses a special well plate accessory for magnetic intervention in three-dimensional cell culture, including a body 1, and further comprising:

[0036] Adsorption mechanism 2 and confinement mechanism 3; wherein:

[0037] The adsorption mechanism 2 includes a top cover 21 located at the upper end of the body 1. An adsorption component 22 is provided inside the top cover 21. The adsorption component 22 is used to provide magnetic field adsorption, so that the magnetic microspheres or magnetic hydrogels float in the culture medium and remain suspended, so that the cells can fully contact the culture medium and ensure the stability of the three-dimensional culture system.

[0038] The limiting mechanism 3 includes a balancing component 31 located at the lower part of the top cover 21, and a limiting component 32 located at the lower end of the balancing component. The limiting component 32 prevents magnetic microspheres or magnetic hydrogels from floating to the surface of the culture medium, thus avoiding affecting the uniformity of cell growth.

[0039] like Figure 5 , 6 As shown, in this embodiment, the adsorption component 22 includes a magnet groove 221 formed inside the center of the top cover 21. A magnet sheet 222 is fixedly installed inside the magnet groove 221. The magnet sheet 222 can provide a stable magnetic field for the culture system, so that the magnetic microspheres or magnetic hydrogels form a three-dimensional distribution under the action of the magnetic field, which helps to uniformly culture cells. The magnet sheet 222 encapsulated inside the magnet groove 221 has the following functions: 1. It isolates the magnet sheet 222 from the external environment, avoiding the risk of contamination caused by direct contact with the culture medium; 2. The fixedly installed magnet can ensure a stable magnetic field strength, preventing the magnet from being displaced due to external forces during the experiment; 3. It ensures that the magnetic field strength generated by all magnets in the same batch of experiments is consistent, reducing the magnetic field deviation between different experimental units, thereby reducing experimental errors caused by uneven magnetic fields.

[0040] like Figure 1 , 5 As shown, in this embodiment, the limiting component 32 includes a gas-liquid balance tank 321 that extends through the periphery of the balancing component 31. The gas-liquid balance tank 321 ensures that the liquid culture medium can freely and smoothly enter and exit the balancing component 31. During the process of the main body 1 being placed into the small chamber 41 of the well plate 4, it avoids the generation of gas cavities due to air pressure, which would cause the magnetic microspheres or magnetic hydrogels to float to the surface of the culture medium after they have floated up. The lower end of the balancing component 31 is provided with a barrier net 322, which is used to limit the magnetic microspheres or magnetic hydrogels from floating to the surface of the culture medium under the action of the magnetic field, thus affecting the uniformity of cell growth.

[0041] like Figure 1 , 2 As shown in Figure 3, in this embodiment, there are multiple gas-liquid balance tanks 321, which are evenly distributed around the balance component 31. The even distribution can ensure that the culture medium flows evenly inside the entire balance component 31, and prevent local liquid stagnation or slow flow rate from affecting the cell state.

[0042] The mesh size of the barrier net 322 is slightly smaller than the particle size of the magnetic microspheres or magnetic hydrogels, ensuring that the liquid culture medium can pass through smoothly, while effectively limiting the floating of the magnetic microspheres or magnetic hydrogels.

[0043] like Figure 1 , 2As shown in Figures 3, 4, and 5, in this embodiment, the main body 1 has three specifications: 6-well plate, 12-well plate, and 24-well plate. The inner diameter of the small chambers 41 of the three types of plates is different. Therefore, the size of the main body 1 is provided in three specifications corresponding to the inner diameter of the three types of small chambers 41. The outer diameter of its top cover 21 is slightly larger than the inner diameter of the corresponding small chamber 41 of the plate. This ensures that after the main body 1 is placed in the small chamber 41, the top cover 21 can be locked at the opening of the small chamber 41, preventing the main body 1 from sinking to the bottom of the small chamber 41. The outer diameter of the balancing component 31 is slightly smaller than the inner diameter of the corresponding small chamber 41 of the plate. This ensures that the main body 1 can be placed exactly in the small chamber 41 of the corresponding specification of the plate without affecting the flow of the culture medium. At the same time, it can effectively prevent magnetic microspheres or magnetic hydrogels from floating to the surface of the culture medium.

[0044] In this embodiment, the top cover 21 is made of a transparent material that is corrosion-resistant and does not affect magnetic field conduction. This effectively prevents chemical corrosion, improves the durability of the device, and ensures the effective transmission of the magnetic field, allowing the magnetic microspheres or magnetic hydrogels to obtain a stable magnetic field. The transparent material also allows the experimenter to observe the suspension state of the magnetic microspheres or magnetic hydrogels in the chamber 41 through the top cover 21 without removing the main body 1. The balancing component 31 is made of a transparent material with good biocompatibility and a certain mechanical strength, which can avoid adverse reactions with the culture medium or cells, ensuring the biosafety of the experiment. At the same time, it has sufficient mechanical strength to prevent deformation or damage during operation. The transparent material also ensures that the experimenter's observation of the suspension state of the magnetic microspheres or magnetic hydrogels in the chamber 41 through the top cover 21 is not affected. The barrier net 322 is made of a highly permeable and biodegradable material, ensuring that the culture medium can flow smoothly through the mesh, and will not produce biological pollution after long-term use.

[0045] The working principle and usage process of this technical solution are as follows: During use, the culture medium and magnetic microspheres or magnetic hydrogel are first added into the small chamber 41 of the appropriate well plate 4. The appropriate size of the body 1 is selected according to the experimental requirements and installed in the small chamber 41 of the well plate 4, so that its top cover 21 is stably inserted into the opening of the small chamber 41 of the well plate 4.

[0046] The gas-liquid balance tank 321 of the balancer 31 ensures that the culture medium can smoothly enter the interior of the balancer 31. Under the action of the magnet 222 inside the top cover 21, the magnetic microspheres or magnetic hydrogels can rise and suspend stably. At the same time, due to the presence of the barrier net 322, the magnetic microspheres or magnetic hydrogels will not float to the surface of the culture medium due to the attraction of the magnetic field. At this time, the magnetic microspheres or magnetic hydrogels can maintain a good suspension state, thereby effectively maintaining the three-dimensional culture environment of cells.

[0047] Finally, after the culture is complete, the entire microsphere 1 can be removed from the well plate 4. Researchers can then recover the magnetic microspheres or magnetic hydrogel using a pipette or centrifugation method and conduct subsequent cell analysis experiments.

[0048] The present invention has been further described above with reference to the embodiments, but the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A magnetic intervention cell three-dimensional culture dedicated well plate accessory comprising a body (1), characterized in that: Also include: Adsorption mechanism (2) and limiting mechanism (3);Wherein: Adsorption mechanism (2) includes the top cover (21) provided on the upper end of the body (1), the top cover (21) is provided with adsorption assembly (22); Limiting mechanism (3) includes the balance piece (31) provided on the lower part of the top cover (21), the lower end of the balance piece (31) is provided with limiting assembly (32).

2. The magnetic intervention cell three-dimensional culture special well plate accessory of claim 1, wherein: The adsorption assembly (22) includes the magnet slot (221) opened in the center of the top cover (21), and the magnet piece (222) is fixedly installed in the magnet slot (221).

3. The magnetic intervention cell three-dimensional culture special well plate accessory of claim 1, wherein: The limiting assembly (32) includes the gas-liquid balance groove (321) penetratingly opened in the circumferential side of the balance piece (31), and the lower end of the balance piece (31) is provided with the blocking net (322).

4. The magnetic intervention cell three-dimensional culture special well plate accessory of claim 3, wherein: The number of the gas-liquid balance groove (321) is multiple, and is uniformly distributed on the circumferential side of the balance piece (31); The mesh size of the blocking net (322) is slightly smaller than the particle size of the magnetic microspheres or the magnetic hydrogel.

5. The magnetic intervention cell three-dimensional culture special well plate accessory of claim 1, wherein: The body (1) has three specifications of 6 hole plate, 12 hole plate and 24 hole plate, the outer diameter of the top cover (21) is slightly larger than the inner diameter of the chamber (41) of the corresponding hole plate (4), and the outer diameter of the balance piece (31) is slightly smaller than the inner diameter of the chamber (41) of the corresponding hole plate (4).

6. The magnetic intervention cell three-dimensional culture special well plate accessory of claim 3, wherein: The top cover (21) is made of transparent material with corrosion resistance and does not affect the conduction of magnetic field;The balance piece (31) is made of transparent material with good biocompatibility and certain mechanical strength;The blocking net (322) is made of material with high water permeability and biodegradation resistance.

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