Sheet carrier loading and culturing device for cell culture
By designing a cell culture device that supports sheet-like carrier membranes, the problem of sheet-like carriers bending and tilting in the culture medium was solved, achieving uniform cell attachment and high yield, and improving the quality of cell products.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-23
- Publication Date
- 2026-04-03
AI Technical Summary
In existing technologies, sheet-like carriers tend to bend and tilt in the culture medium, resulting in uneven cell attachment and affecting the yield and quality of cell products.
A sheet-like carrier loading and culture device for cell culture was designed, including a culture mechanism and a sheet-like carrier loading mechanism. The sheet-like carrier membrane is supported by a suspension frame and a limiting cylinder to prevent it from bending or tilting in the culture medium, and is easy to install and disassemble by a stirring blade and a threaded sleeve.
This method achieves uniform cell attachment on sheet-like carrier membranes, improves the yield and quality of cell products, and facilitates the installation and cleaning of loading boxes.
Smart Images
Figure CN224077425U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of cell culture technology, specifically relating to a sheet-like carrier loading and culture device for cell culture. Background Technology
[0002] Cell culture technology is an indispensable key technology in modern biotechnology, pharmaceuticals, and vaccine production. Traditional cell culture methods, such as those using roller bottles or cell factories, while meeting the needs of cell culture to a certain extent, have gradually revealed limitations as scientific research has deepened and industrial production scales up. To improve the efficiency and density of cell culture, researchers have begun to explore new cell culture methods. Among them, sheet-like carrier culture technology has attracted much attention due to its unique advantages. Sheet-like carrier culture technology is a cell culture method that uses specially designed sheet-like carriers as a cell growth substrate. These sheet-like carriers typically have a large surface area to volume ratio, providing more cell attachment sites and thus supporting higher-density cell culture. Furthermore, sheet-like carriers can be customized to suit different types of cells and culture conditions. During cell culture, cells adhere to the surface of the sheet-like carrier and grow, forming a dense cell layer. This culture method not only promotes cell growth and proliferation but also improves the yield and quality of cell products.
[0003] However, in the current process of culturing cells using sheet-like carriers, because these carriers are usually made of flexible and thin materials, they are prone to bending, tilting, and collapsing when immersed in the culture medium. This results in cells not attaching evenly to the carrier, leading to uneven cell growth and affecting the yield and quality of cell products. To address this problem, the applicant proposes a sheet-like carrier loading and culturing device for cell culture. Utility Model Content
[0004] The purpose of this invention is to provide a sheet-like carrier loading and culture device for cell culture, which facilitates the installation of the sheet-like carrier membrane and supports the sheet-like carrier membrane during cell culture, preventing the sheet-like carrier membrane from bending or tilting. This allows cells to attach evenly to the sheet-like carrier membrane, enhancing the balanced effect of cell growth and improving the yield and quality of cell products.
[0005] To achieve the above objectives, this utility model provides the following technical solution:
[0006] A cell culture sheet-like carrier loading and culture device, comprising:
[0007] A culture mechanism and a sheet-like carrier loading mechanism assembled inside the culture mechanism;
[0008] The culture apparatus includes a culture tank, and a cover plate is connected to the top of the culture tank;
[0009] The sheet carrier loading mechanism includes a sheet carrier loading chamber suspended at the bottom of the cover plate. The sheet carrier loading chamber is suspended from the cover plate by a suspension frame. A fixing frame is fixedly installed inside the sheet carrier loading chamber. A limiting cylinder is connected inside the fixing frame. A loading box is inserted between the limiting cylinder and the fixing frame. The two sides of the loading box are hollow. A sheet carrier film is inserted inside the loading box.
[0010] Preferably, the cover plate has symmetrically distributed mounting holes, and the suspension bracket is inserted into the mounting holes and fixed with bolts.
[0011] Preferably, a rotating rod is rotatably mounted at the center of the cover plate, and the rotating rod extends through the limiting cylinder.
[0012] Preferably, the outer periphery of the rotating rod is connected to a threaded sleeve by a helical thread, and a plurality of stirring blades arranged in a ring array are fixedly installed on the outer periphery of the threaded sleeve.
[0013] Preferably, the top of the cover plate is connected to an injection port on one side of the rotating rod, and the top of the cover plate is fitted with a sealing cap on the other side of the rotating rod.
[0014] Preferably, the cover plate is fixed to the culture tank by a plurality of fixing bolts, which are distributed in a ring array at equal intervals.
[0015] Preferably, the bottom of the loading box has multiple through holes arranged in a straight line at equal intervals. The limiting cylinder is installed in the loading box by insertion through an installation groove. The inner wall of the fixing frame has a limiting groove for installing the loading box.
[0016] Compared with the prior art, the beneficial effects of this utility model are:
[0017] (1) This utility model is equipped with a sheet-like carrier loading mechanism, which can insert the loading box between the limiting cylinder and the fixing frame, and can be installed and disassembled quickly and conveniently. At the same time, by inserting the sheet-like carrier membrane into the loading box, the loading box can support the sheet-like carrier membrane, so that the sheet-like carrier membrane is not prone to displacement or bending and tilting when immersed in the culture medium, so that the cells can be evenly attached to the sheet-like carrier membrane for growth, thereby improving the yield and quality of cell products.
[0018] (2) The present invention has a threaded sleeve on the rotating rod, which allows the stirring blade to be easily installed and disassembled with the rotating rod. This allows the workers to remove the stirring blade for cleaning and maintenance, bringing more convenience to the cleaning work. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the overall three-dimensional structure of this utility model;
[0020] Figure 2 This is a cross-sectional structural diagram of the present invention;
[0021] Figure 3 This is a schematic diagram of the cover plate structure of this utility model;
[0022] Figure 4 This is a schematic diagram of the sheet-like carrier loading mechanism of this utility model;
[0023] Figure 5 This is a schematic diagram of the limiting cylinder structure of this utility model;
[0024] Figure 6 This is a bottom view of the loading box structure of this utility model;
[0025] In the diagram: 1. Culture mechanism; 11. Culture tank; 12. Cover plate; 13. Injection port; 14. Rotating rod; 15. Sealing cap; 16. Mounting hole; 17. Threaded sleeve; 18. Stirring blade; 19. Spiral pattern; 110. Fixing bolt; 2. Sheet carrier loading mechanism; 21. Limiting cylinder; 22. Loading box; 23. Sheet carrier membrane; 24. Fixing frame; 25. Suspension frame; 26. Through hole; 27. Mounting groove; 28. Limiting groove; 29. Sheet carrier loading chamber. Detailed Implementation
[0026] The technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0027] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0028] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0029] Example 1:
[0030] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 As shown, a sheet-like carrier loading and culture device for cell culture includes:
[0031] The culture mechanism 1 and the sheet-like carrier loading mechanism 2 assembled inside the culture mechanism 1;
[0032] The culture apparatus 1 includes a culture tank 11, and a cover plate 12 is connected to the top of the culture tank 11;
[0033] The sheet carrier loading mechanism 2 includes a sheet carrier loading chamber 29 suspended at the bottom of the cover plate 12. The sheet carrier loading chamber 29 is suspended from the cover plate 12 by a suspension frame 25. A fixing frame 24 is fixedly installed inside the sheet carrier loading chamber 29. A limiting cylinder 21 is connected inside the fixing frame 24. A loading box 22 is inserted between the limiting cylinder 21 and the fixing frame 24. The two sides of the loading box 22 are hollow. A sheet carrier film 23 is inserted inside the loading box 22.
[0034] As can be seen from the above, by inserting the loading box 22 between the limiting cylinder 21 and the fixing frame 24 during use, the loading box 22 can be quickly installed and disassembled relatively easily. By inserting the sheet-like carrier membrane 23 into the loading box 22 and suspending and fixing the sheet-like carrier loading mechanism 2 to the bottom of the cover plate 12 using the suspension bracket 25, the installation and disassembly operations can be easily performed. By placing the sheet-like carrier loading mechanism 2 into the culture tank 11 and making the cover plate 12 fit against the top of the culture tank 11, the cover plate 12 and the culture tank 11 are fixedly sealed. The culture medium is injected into the culture tank 11, allowing it to enter the sheet carrier loading chamber 29 from bottom to top through the opening at the bottom. This allows the sheet carrier membrane 23 to be immersed in the culture medium, enabling the cells in the culture medium to attach to the surface of the sheet carrier membrane 23. The loading box 22 supports the sheet carrier membrane 23, preventing it from shifting, bending, or tilting under the immersion in the culture medium. This allows the cells to attach evenly to the sheet carrier membrane 23 for growth, improving the yield and quality of cell products.
[0035] Depend on Figure 2 , Figure 3 and Figure 4 It can be seen that the cover plate 12 has symmetrically distributed mounting holes 16, and the suspension bracket 25 is inserted into the mounting holes 16 and fixed with bolts.
[0036] As can be seen from the above, by inserting the suspension bracket 25 into the mounting hole 16, the top of the suspension bracket 25 can extend out of the cover plate 12. The suspension bracket 25 is a threaded bolt, which can be connected to the top of the suspension bracket 25 with bolts. The suspension bracket 25 and the cover plate 12 can be fixed together, which facilitates the installation of the sheet-like carrier loading mechanism 2.
[0037] For details, please refer to Figure 2 As shown, a rotating rod 14 is rotatably installed at the center of the cover plate 12, and the rotating rod 14 passes through the limiting cylinder 21.
[0038] As can be seen from the above, the cover plate 12 extends from the top of the rotating rod 14, and an external motor can be connected to the rotating rod 14 to facilitate the rotation of the rotating rod 14. The limiting sleeve 21 is sleeved around the rotating rod 14, so as not to affect the installation operation of the sheet carrier loading mechanism 2.
[0039] For details, please refer to Figure 3 As shown, the outer periphery of the rotating rod 14 is connected to a threaded sleeve 17 by a spiral thread 19, and multiple stirring blades 18 arranged in a ring array are fixedly installed on the outer periphery of the threaded sleeve 17.
[0040] As can be seen from the above, the threaded sleeve 17 can be easily installed on the rotating rod 14 through the spiral pattern 19, which facilitates the installation and disassembly of the stirring blade 18. By disassembling the stirring blade 18 from the rotating rod 14, the sheet carrier loading mechanism 2 can be easily installed and disassembled.
[0041] Example 2:
[0042] refer to Figure 2 and Figure 3 As shown, the top of the cover plate 12 is connected to an injection port 13 on one side of the rotating rod 14, and the top of the cover plate 12 is plugged with a sealing cap 15 on the other side of the rotating rod 14.
[0043] As can be seen from the above, the culture medium can be easily injected into the culture tank 11 through the injection port 13, which facilitates cell culture operations. By opening the sealing cap 15, the staff can easily use tools to remove the sheet-like carrier membrane 23, which facilitates sampling and analysis operations.
[0044] refer to Figure 2 and Figure 3 As shown, the cover plate 12 is fixed to the culture tank 11 by multiple fixing bolts 110, which are distributed in a ring array at equal intervals.
[0045] As can be seen from the above, the cover plate 12 and the culture tank 11 can be easily fixed and sealed by rotating the fixing bolt 110. Multiple fixing bolts 110 can evenly fix the cover plate 12, which can effectively ensure the sealing effect.
[0046] refer to Figure 5 As shown, the bottom of the loading box 22 has multiple through holes 26, which are arranged in a straight line at equal intervals. The limiting cylinder 21 is connected to the loading box 22 by an installation groove 27. The inner wall of the fixing frame 24 has a limiting groove 28 for the loading box 22 to be installed.
[0047] As can be seen from the above, when the culture medium is introduced into the culture tank 11, the culture medium can enter the loading box 22 from the bottom through the through hole 26, so that the culture medium can be evenly contacted with the sheet carrier membrane 23. By inserting the loading box 22 into the installation groove 27, the loading box 22 can be easily installed. The limiting groove 28 can limit the loading box 22, so that the loading box 22 will not shake, which facilitates the installation and disassembly of the loading box 22.
[0048] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A device for loading and culturing sheet-like carriers for cell culture, characterized in that, The utility model relates to a culture device, which comprises a culture mechanism (1) and a sheet carrier loading mechanism (2) assembled inside the culture mechanism (1). The culture mechanism (1) comprises a culture tank (11), and a cover plate (12) is connected to the top of the culture tank (11). The sheet carrier loading mechanism (2) comprises a sheet carrier loading chamber (29) suspended from the bottom of the cover plate (12), which is suspended and installed between the cover plate (12) through a suspension frame (25), and a fixed frame (24) is fixedly installed inside the sheet carrier loading chamber (29), a limiting cylinder (21) is connected to the inside of the fixed frame (24), a loading box (22) is inserted and installed between the limiting cylinder (21) and the fixed frame (24), and both sides of the loading box (22) are hollow, and a sheet carrier film (23) is inserted into the inside of the loading box (22). Symmetrical mounting holes (16) are formed in the cover plate (12), the suspension frame (25) is inserted into the mounting holes (16) and fixed through bolts.
2. The cell culture sheet carrier loading and culture device according to claim 1, wherein: A rotating shaft (14) is rotatably installed at the center of the cover plate (12), and the rotating shaft (14) penetrates through the limiting cylinder (21).
3. The cell culture sheet carrier loading and culture device according to claim 1, wherein: A threaded sleeve (17) is threadedly connected to the periphery of the rotating shaft (14) through a helical thread (19), and a plurality of stirring blades (18) are fixedly installed around the threaded sleeve (17) in an annular array.
4. The cell culture sheet carrier loading and culture device according to claim 3, wherein: A liquid injection port (13) is connected to the top of the cover plate (12) on one side of the rotating shaft (14), and a sealing cover (15) is inserted into the top of the cover plate (12) on the other side of the rotating shaft (14).
5. The cell culture sheet carrier loading and culture device according to claim 3, wherein: The cover plate (12) and the culture tank (11) are fixed through a plurality of fixing bolts (110), which are arranged in an annular array at equal intervals.
6. The cell culture sheet carrier loading and culture device according to claim 1, wherein: A plurality of through holes (26) are formed in the bottom of the loading box (22), which are arranged in a linear array at equal intervals, the limiting cylinder (21) and the loading box (22) are inserted and installed through an installation groove (27), and a limiting groove (28) for installing the loading box (22) is formed in the inner wall of the fixed frame (24).
7. The cell culture sheet carrier loading and culturing device according to claim 1, wherein: