Sephadex purification and separation collector

Abstract

The utility model belongs to the technical field of separation and purification, and particularly relates to a sephadex purification and separation collector, which comprises a chromatographic column and a container, and further comprises a base, a sephadex inlet, a sephadex outlet, a sephadex outlet, a sephadex inlet and a sephadex outlet, the peristaltic pump is fixed at the top of the base; one end of the tee joint is connected with a liquid outlet of the peristaltic pump through a hose, and the other end of the tee joint is connected with an electromagnetic valve; the liquid outlet pipe is fixedly connected with the electromagnetic valve; a loading turntable; the driving motor is fixed on the base and is used for driving the material loading turntable to rotate; according to the device, the material loading turntable is used for bearing a plurality of different containers, and the driving motor is used for driving the material loading turntable to rotate, so that different containers can be quickly switched to correspond to the liquid outlet pipes, and eluents at different positions can be conveniently collected; and through the designed peristaltic pump, the eluent can be pumped out in time, and the problem caused by overlong elution time can also be avoided.

Description

Technical Field

[0001] This invention belongs to the field of separation and purification technology, specifically relating to a dextran gel purification, separation, and collection device. Background Technology

[0002] Dextran gel purification and separation is a commonly used biochemical separation technique. Dextran gel is a high molecular weight polymer with a three-dimensional network structure, formed by cross-linking dextran and a cross-linking agent. It contains many pores of different sizes, which act like molecular sieves. When a mixture containing molecules of different sizes passes through a dextran gel column, substances whose molecular size matches the gel pores enter the gel particles and flow more slowly within the column; while larger molecules cannot enter the pores and flow only in the spaces between the gel particles, passing through the gel column more quickly.

[0003] Chromatography columns are typically used in an inclined or vertical position. The sample is added to the column, separated and filtered through the dextran gel column, and finally eluted. The eluent is then collected for analysis.

[0004] Existing purification and separation collectors often require manual replacement of different collection containers for eluent at different locations, resulting in low efficiency and excessively long elution times at some locations. This can lead to peak broadening, reduced resolution, and worsened tailing.

[0005] To address the aforementioned problems, this invention proposes a dextran gel purification, separation, and collection device. Utility Model Content

[0006] To address the aforementioned problems in the existing technology, this utility model provides a dextran gel purification, separation, and collection device, which is convenient to use and has high safety performance.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a dextran gel purification, separation, and collection device, comprising a chromatography column and a container, and further comprising:

[0008] The base, wherein the chromatography column is mounted on top of the base in an inclined or vertical manner;

[0009] A peristaltic pump, which is fixed to the top of the base, and the inlet of the peristaltic pump is connected to the chromatography column via a hose;

[0010] A three-way valve, one end of which is connected to the outlet of the peristaltic pump via a hose, and the other end is connected to a solenoid valve;

[0011] A liquid outlet pipe, which is fixedly connected to the solenoid valve;

[0012] A material-carrying turntable is rotatably connected to the base, and a plurality of placement slots are machined on the top surface of the material-carrying turntable and are equally spaced along the circumferential direction, and the container is placed in the placement slots;

[0013] A drive motor is fixed on the base and is used to drive the material turntable to rotate.

[0014] As a preferred embodiment of this utility model, the chromatography column includes:

[0015] cylinder;

[0016] The cylinder is fitted with plugs at both ends by means of threaded engagement.

[0017] Dextran gel column, wherein the dextran gel column is filled inside the cylinder;

[0018] A filter screen, which is fixed inside the plug with bolts.

[0019] As a preferred technical solution of this utility model, it also includes:

[0020] A sealing cap is provided on the plug, which has an exhaust port communicating with the cylinder. The sealing cap is installed on the exhaust port by means of threaded engagement.

[0021] As a preferred technical solution of this utility model, it also includes:

[0022] A fixing clamp is fixed to the top surface of the base, and an arc-shaped groove for embedding the chromatography column is machined on the fixing clamp.

[0023] A movable clamping block is distributed relative to the fixed clamping block, and a V-groove is machined on the movable clamping block;

[0024] An adjustment mechanism controls the movement of the movable clamping block to bring it closer to or away from the fixed clamping block.

[0025] As a preferred embodiment of this utility model, the adjustment mechanism includes:

[0026] Support columns, four of which are diagonally fixed to the top of the fixing block;

[0027] A limiting plate, which is fixed to the top of the support column;

[0028] An adjusting screw is provided, which is screwed onto the limiting plate by means of a thread, and one end of the adjusting screw is rotatably connected to the movable clamping block by means of a bearing;

[0029] A handle, the handle being fixed to the end of the adjusting screw away from the movable clamping block;

[0030] Two guide rods are symmetrically fixed to the outer wall of the movable clamping block and pass through the limiting plate.

[0031] As a preferred technical solution of this utility model, it also includes:

[0032] A rubber liner is bonded and fixed to the inner walls of both the arc-shaped groove and the V-shaped groove.

[0033] As a preferred technical solution of this utility model, it also includes:

[0034] A manual valve, which is fixed to the tee.

[0035] As a preferred technical solution of this utility model, it also includes:

[0036] A through-impeller sight glass is installed at the inlet end of the chromatography column.

[0037] Compared with the prior art, the beneficial effects of this utility model are:

[0038] In this invention, a material-carrying turntable is used to support multiple different containers. The turntable is driven by a drive motor to rotate, so as to quickly switch between different containers and their corresponding liquid outlet pipes, making it convenient to collect eluent from different locations. The designed peristaltic pump can extract the eluent in a timely manner, which can also avoid the problem caused by excessive elution time.

[0039] Other additional advantages and beneficial effects of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this invention. Attached Figure Description

[0040] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0041] Figure 1 This is a schematic diagram of the structure of this utility model;

[0042] Figure 2 This utility model Figure 1 Enlarged structural diagram at point A in the diagram;

[0043] Figure 3 This utility model Figure 1 Enlarged structural diagram at point B in the diagram;

[0044] Figure 4This utility model Figure 1 Enlarged structural diagram at point C;

[0045] Figure 5 This utility model Figure 1 A schematic diagram of the enlarged structure of the adjustment mechanism in the middle;

[0046] Figure 6 This is a schematic diagram of the cross-sectional structure of the chromatography column in this utility model.

[0047] In the diagram: 1. Base; 101. Cylinder; 102. Plug; 103. Dextran gel column; 104. Filter screen; 105. Exhaust port; 106. Sealing cap; 2. Chromatography column; 3. Peristaltic pump; 4. Adjustment mechanism; 41. Support column; 42. Limiting plate; 43. Adjusting screw; 44. Handle; 45. Guide rod; 5. Straight-through impeller sight glass; 6. Fixed clamp; 61. Arc groove; 7. Movable clamp; 71. V-groove; 8. Rubber liner; 9. T-joint; 10. Manual valve; 11. Solenoid valve; 12. Discharge pipe; 13. Material turntable; 131. Placement tank; 14. Drive motor; 15. Container. Detailed Implementation

[0048] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0049] Please see Figures 1-6 The present invention provides the following technical solution: a dextran gel purification and separation collector, including a chromatography column 2 and a container 15, and further including: a base 1, a peristaltic pump 3, a three-way valve 9, a liquid outlet pipe 12, a material carrier turntable 13 and a drive motor 14.

[0050] Furthermore, by Figure 1 , Figure 3 and Figure 4As shown, in this embodiment, the chromatography column 2 is installed on the top of the base 1 in an inclined or vertical position. The peristaltic pump 3 is fixed to the top of the base 1, and the inlet of the peristaltic pump 3 is connected to the chromatography column 2 via a hose. One end of the tee 9 is connected to the outlet of the peristaltic pump 3 via a hose, and the other end is connected to the solenoid valve 11. The outlet pipe 12 is fixedly connected to the solenoid valve 11. The material turntable 13 is rotatably connected to the base 1, and multiple placement slots 131 are equally spaced along the circumferential direction on the top surface of the material turntable 13. The container 15 is placed in the placement slot 131. The drive motor 14 is fixed on the base 1 and is used to drive the material turntable 13 to rotate. With the above scheme, when using it, the chromatography column 2 is first installed on the top of the base 1 in an inclined or vertical position (the chromatography column 2 of this utility model is set in an inclined position; the specific installation state needs to be determined according to actual needs, and the inclination angle of the chromatography column 2 is also considered). The degree of separation also needs to be determined according to actual needs. Then, the sample is added to the chromatography column 2 and filtered and separated using the chromatography column 2. Then, the eluent is introduced for elution. The sample with larger particles is discharged first. At this time, the peristaltic pump 3 is started and the solenoid valve 11 is opened. The sample eluent passes through the peristaltic pump 3, the solenoid valve 11 and the outlet tube 12 into the container 15 below for collection. When collecting the eluent from other positions, the drive motor 14 is started to drive the material carrier turntable 13 to rotate, so that the different containers 15 rotate to the bottom of the outlet tube 12. This utility model uses the material carrier turntable 13 to carry multiple different containers 15. The drive motor 14 drives the material carrier turntable 13 to rotate, so as to quickly switch the different containers 15 to the outlet tube 12, which is convenient for collecting the eluent from different positions. The peristaltic pump 3 designed can extract the eluent in time, which can also avoid the problem caused by excessive elution time.

[0051] Optionally, by Figure 1 and Figure 6 As shown in this embodiment, the chromatography column 2 includes: a cylinder 101, a plug 102, a dextran gel column 103, and a filter screen 104. The plug 102 is installed at both ends of the cylinder 101 by means of threaded engagement. The dextran gel column 103 is filled inside the cylinder 101. The filter screen 104 is fixed inside the plug 102 by bolts. With the above scheme, in use, the filter screen 104 is used to initially filter the sample to remove impurities in the sample, and then the sample is purified and separated by the dextran gel column 103.

[0052] Preferably, by Figure 1 and Figure 6As shown, this embodiment also includes: a sealing cap 106, and an exhaust port 105 communicating with the cylinder 101 is provided on the plug 102. The sealing cap 106 is installed on the exhaust port 105 by screwing on. With the above solution, when loading the column, the sealing cap 106 is unscrewed, and after the dextran gel is loaded into the cylinder 101, the air in the cylinder 101 is discharged from the exhaust port 105. If necessary, the exhaust port 105 can also be connected to an air pump to extract the air in the cylinder 101.

[0053] Preferably, by Figure 1 and Figure 2 As shown, this embodiment also includes: a fixed clamping block 6, a movable clamping block 7, and an adjustment mechanism 4. The fixed clamping block 6 is fixed to the top surface of the base 1, and an arc-shaped groove 61 for embedding the chromatography column 2 is machined on the fixed clamping block 6. The movable clamping block 7 is distributed relative to the fixed clamping block 6, and a V-shaped groove 71 is machined on the movable clamping block 7. The adjustment mechanism 4 controls the movement of the movable clamping block 7 so that the movable clamping block 7 moves closer to or away from the fixed clamping block 6. With the above scheme, when in use, the chromatography column 2 is placed in the arc-shaped groove 61, and then the movable clamping block 7 is controlled to move down by the adjustment mechanism 4. The movable clamping block 7 and the fixed clamping block 6 are used to clamp the chromatography column 2 to ensure the stability of the chromatography column 2, and it is applicable to chromatography columns 2 of different sizes.

[0054] Optionally, by Figure 1 , Figure 2 and Figure 5 As shown, in this embodiment, the adjustment mechanism 4 includes: support columns 41, limiting plate 42, adjusting screw 43, handle 44, and guide rod 45. The four support columns 41 are diagonally fixed to the top of the fixed clamping block 6. The limiting plate 42 is fixed to the top of the support columns 41. The adjusting screw 43 is screwed onto the limiting plate 42, and one end of the adjusting screw 43 is rotatably connected to the movable clamping block 7 via a bearing. The handle 44 is fixed to the end of the adjusting screw 43 away from the movable clamping block 7. The two guide rods 45 are symmetrically fixed to the outer wall of the movable clamping block 7 and pass through the limiting plate 42. With the above scheme, in use, the adjusting screw 43 is rotated by the handle 44. The adjusting screw 43 drives the movable clamping block 7 to move under the screwing action, and the two guide rods 45 guide the movable clamping block 7 to ensure the stability of the movable clamping block 7.

[0055] Preferably, by Figure 1 and Figure 2 As shown, in this embodiment, it also includes a rubber liner 8. The rubber liner 8 is bonded and fixed to the inner walls of both the arc groove 61 and the V-groove 71. With the above solution, the rubber liner 8 is flexible during use. By clamping the chromatography column 2 with the rubber liner 8, the stability and safety of the chromatography column 2 are further improved.

[0056] Optionally, by Figure 1and Figure 3 As shown, this embodiment also includes a manual valve 10, which is fixed on the tee 9. With the above solution, the manual valve 10 is in the closed state during operation. When temporary sampling is required, the manual valve 10 is opened and a temporary sampling device, such as a syringe, is used to take a sample from the tee 9.

[0057] Preferably, by Figure 1 As shown, this embodiment also includes a straight-through impeller sight glass 5. The straight-through impeller sight glass 5 is installed at the liquid inlet end of the chromatography column 2. With the above scheme, when there is medium flowing in the pipeline, the medium directly acts on the impeller of the straight-through impeller sight glass 5 through the set straight-through impeller sight glass 5, driving the impeller to rotate. The rotation speed of the impeller is closely related to the flow rate of the medium. By observing the rotation of the impeller through the window of the straight-through impeller sight glass 5, it is possible to intuitively determine whether the medium in the pipeline is flowing and how fast it is flowing.

[0058] It should be noted that the through impeller sight glass 5 is a very common component, and its specific composition and principle are all publicly available technologies. For details, please refer to the through impeller sight glass 5 in the existing technology. It will not be elaborated on further in this article.

[0059] It should be noted that the peristaltic pump 3, solenoid valve 11 and drive motor 14 are all commercially available conventional devices with built-in power switches. Those skilled in the art can make conventional selections according to their needs. Their working principles are common knowledge known to those skilled in the art and have been fully disclosed in the prior art, so they will not be elaborated on further in this article.

[0060] The circuit connection involved in this utility model is a common method used by those skilled in the art, and technical inspiration can be obtained through a limited number of experiments. It belongs to the widely used prior art.

[0061] Components not described in detail in this article are existing technologies.

[0062] The working principle and usage process of this utility model: When using the dextran gel purification and separation collector of this utility model, first install the chromatography column 2 in an inclined or vertical position on the top of the base 1, then add the sample into the chromatography column 2, and use the chromatography column 2 for filtration and separation.

[0063] Then, eluent is introduced for elution. Samples with larger particles are discharged first. At this time, peristaltic pump 3 is started and solenoid valve 11 is opened. The sample eluent passes through peristaltic pump 3, solenoid valve 11 and outlet pipe 12 into container 15 below for collection.

[0064] When collecting eluent from other locations, start the drive motor 14 to drive the material turntable 13 to rotate, so that the different containers 15 rotate to below the outlet pipe 12.

[0065] This utility model uses a material-carrying turntable 13 to carry multiple different containers 15. The turntable 13 is driven to rotate by a drive motor 14 to quickly switch between different containers 15 and the corresponding liquid outlet pipes 12, making it convenient to collect the eluent from different positions. The designed peristaltic pump 3 can extract the eluent in time, which can also avoid the problem caused by excessive elution time.

[0066] In another aspect of this utility model, by setting a straight-through impeller sight glass 5, when there is a medium flowing in the pipeline, the medium directly acts on the impeller of the straight-through impeller sight glass 5, driving the impeller to rotate. The rotation speed of the impeller is closely related to the flow rate of the medium. By observing the rotation of the impeller through the window of the straight-through impeller sight glass 5, it is possible to intuitively determine whether the medium in the pipeline is flowing and how fast it is flowing.

[0067] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A dextran gel purification and separation collector, comprising a chromatography column (2) and a container (15), characterized in that, Also includes: The base (1) is on top of the chromatography column (2) in an inclined or vertical manner; A peristaltic pump (3) is fixed to the top of the base (1), and the inlet of the peristaltic pump (3) is connected to the chromatography column (2) via a hose. A three-way valve (9) is connected at one end to the outlet of the peristaltic pump (3) via a hose, and at the other end to a solenoid valve (11). The liquid outlet pipe (12) is fixedly connected to the solenoid valve (11). A material-carrying turntable (13) is rotatably connected to the base (1), and a plurality of placement slots (131) are equally spaced along the circumferential direction on the top surface of the material-carrying turntable (13), and the container (15) is placed in the placement slots (131). A drive motor (14) is fixed on the base (1) and is used to drive the material turntable (13) to rotate.

2. The dextran gel purification, separation, and collection device according to claim 1, characterized in that: The chromatography column (2) comprises: Cylinder (101); The plugs (102) are installed at both ends of the cylinder (101) by means of threaded engagement. A dextran gel column (103) is filled inside the cylinder (101); The filter screen (104) is fixed inside the plug (102) by bolts.

3. The dextran gel purification, separation, and collection device according to claim 2, characterized in that: Also includes: A sealing cap (106) is provided on the plug (102) with an exhaust port (105) communicating with the cylinder (101). The sealing cap (106) is installed on the exhaust port (105) by means of thread engagement.

4. The dextran gel purification, separation, and collection device according to claim 1, characterized in that: Also includes: A fixing clamp (6) is fixed to the top surface of the base (1), and an arc-shaped groove (61) for the chromatography column (2) to be embedded is machined on the fixing clamp (6). Movable clamping block (7), which is distributed relative to the fixed clamping block (6), and a V-groove (71) is machined on the movable clamping block (7); Adjustment mechanism (4) controls the movement of the movable clamping block (7) so that the movable clamping block (7) moves closer to or further away from the fixed clamping block (6).

5. The dextran gel purification, separation, and collection device according to claim 4, characterized in that: The adjustment mechanism (4) includes: Support columns (41), four of the support columns (41) are fixed diagonally to the top of the fixing clamp (6); A limiting plate (42) is fixed to the top of the support column (41); Adjusting screw (43) is screwed onto the limiting plate (42) by means of thread engagement, and one end of the adjusting screw (43) is rotatably connected to the movable clamp (7) by means of bearing; Handle (44), the handle (44) is fixed to the end of the adjusting screw (43) away from the movable clamp (7); Guide rods (45), two of the guide rods (45) are symmetrically fixed to the outer wall of the movable clamp (7) and pass through the limiting plate (42).

6. The dextran gel purification, separation, and collection device according to claim 4, characterized in that: Also includes: The rubber liner (8) is bonded and fixed to the inner walls of both the arc groove (61) and the V-groove (71).

7. The dextran gel purification, separation, and collection device according to claim 1, characterized in that: Also includes: Manual valve (10) is fixed on the tee (9).

8. The dextran gel purification, separation, and collection device according to claim 1, characterized in that: Also includes: A straight-through impeller sight glass (5) is installed at the inlet end of the chromatography column (2).

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