Protein purification mixing container

Abstract

The utility model relates to the technical field of mixing containers, in particular to a protein purification mixing container. The protein purification mixing container comprises a centrifugal tube body and a threaded tube cover, an arc-shaped air bag is installed in an annular groove formed in the centrifugal tube body, and an adjusting component is further installed on the centrifugal tube body. According to the protein purification mixing container provided by the utility model, the centrifugal tube body is inserted into the tube hole of the test tube rack of the vortex oscillator, and then the threaded ring is pulled upwards, so that the arc-shaped air bag expands and extends outwards in the radial direction, a gap between the centrifugal tube body and the tube hole of the test tube rack is quickly filled, and the friction force between the centrifugal tube body and the tube hole of the test tube rack is obviously improved; the centrifugal tube body is stably locked on the test tube rack, so that in the high-speed oscillation process of the vortex oscillator, the risk that the centrifugal tube body shakes, deviates or jumps out is effectively avoided, it is guaranteed that experiments are carried out safely and orderly, meanwhile, the centrifugal tube body is fixed through the structural design of the centrifugal tube body, auxiliary fixing pieces such as a spring clip do not need to be additionally used, and operation is convenient. And the experiment preparation process is simplified.

Description

Technical Field

[0001] This utility model relates to the field of mixing container technology, and in particular to a protein purification mixing container. Background Technology

[0002] In fields such as biopharmaceutical and molecular biology research, protein purification is a crucial experimental step. Mixing containers, as the core equipment used for mixing packing materials and buffer solutions and preparing reaction systems during protein purification, directly impact experimental efficiency and purification results. Currently, many laboratories use centrifuge tubes as mixing containers for protein purification, combined with vortex mixers to achieve oscillation and mixing of the internal solutions.

[0003] However, existing centrifuge tubes have significant drawbacks in practical applications: the tube racks of vortex shakers come in various sizes, requiring constant adjustments and replacements based on the tube diameters. Furthermore, inconsistencies in the tube rack openings often result in gaps between the centrifuge tubes and the rack openings. During high-speed oscillation, this can cause the tubes to wobble, shift, or even jump out of the openings, affecting mixing efficiency and potentially leading to sample leakage, container damage, and other safety hazards. Consequently, manual support is required during mixing. To address this issue, existing solutions typically require additional fixing components such as spring clips to mechanically secure the centrifuge tubes to the rack. However, this method is cumbersome, and the clamping force of the spring clips is difficult to control precisely. Over-clamping can deform the centrifuge tubes, while under-clamping fails to provide effective fixation. The additional fixing components also increase experimental preparation time and operating costs.

[0004] Therefore, it is necessary to provide a new protein purification mixing container to solve the above-mentioned technical problems. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a protein purification mixing container.

[0006] The protein purification mixing container provided by this utility model includes a centrifuge tube and a threaded cap disposed on the centrifuge tube;

[0007] The centrifuge tube body near the bottom has an annular groove and multiple annularly distributed cutting grooves, and the cutting grooves are located above the annular grooves and communicate with each other. The centrifuge tube body above the cutting grooves has an integrally formed threaded part.

[0008] Multiple annularly distributed arc-shaped airbags are installed in the annular groove of the centrifuge tube, and an adjustment component is also installed on the centrifuge tube to drive the arc-shaped airbags to compress and expand.

[0009] Preferably, the adjusting component comprises an upper abutting ring, the upper abutting ring is sleeved on the annular groove of the centrifugal tube body and is in sliding connection with the annular groove, and a plurality of vertical middle connecting plates are fixedly installed on the upper abutting ring in a ring shape, the middle connecting plates are inserted into the corresponding cut grooves and are in sliding connection with the cut grooves, and a threaded ring is arranged on the top of the middle connecting plates.

[0010] Preferably, the bottom of the threaded ring is provided with a rotating ring in rotating connection, and the rotating ring is fixedly connected with the top of the middle connecting plate.

[0011] Preferably, the threaded ring is sleeved on the centrifugal tube body.

[0012] Preferably, the lower bag surface of the arc-shaped air bag is fixedly embedded on the upper abutting ring, the upper bag surface of the arc-shaped air bag is fixedly connected with the inner top wall of the annular groove, and the outer diameter of the arc-shaped air bag is the same as the outer diameter of the centrifugal tube body.

[0013] Preferably, the top pipe opening of the centrifugal tube body is in a threaded pipe opening structure.

[0014] Preferably, a butt piece is fixedly installed on the threaded pipe cover, and a sleeve ring is fixedly installed at the other end of the butt piece, the sleeve ring is sleeved in the annular groove of the centrifugal tube body and is in rotating connection with the annular groove.

[0015] Compared with the related art, the protein purification mixing container has the following beneficial effects:

[0016] The centrifugal tube body is inserted into the tube hole of the vortex oscillator test tube rack, and then the threaded ring is pulled up, so that the arc-shaped air bag is radially expanded and extended outward, and then the threaded ring is screwed along the threaded part, so that the gap between the centrifugal tube body and the tube hole of the test tube rack is quickly filled, the friction between the two is significantly improved, the centrifugal tube body is stably locked on the test tube rack, the risk of shaking, deviation or jumping out of the centrifugal tube body during high-speed oscillation of the vortex oscillator is effectively avoided, the experiment is safely and orderly carried out, the centrifugal tube body is fixed through the structure design, and no additional auxiliary fixing member such as a spring clamp is needed, so that the experimental preparation process is simplified, the operation steps and time cost are reduced, and the experimental efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 A structure schematic view of a preferred embodiment of the protein purification mixing container provided by the utility model;

[0018] Figure 2 A structure schematic view of a preferred embodiment of the protein purification mixing container provided by the utility model; Figure 1 A structure schematic view of a preferred embodiment of the protein purification mixing container provided by the utility model;

[0019] Figure 3 A structure schematic view of a preferred embodiment of the protein purification mixing container provided by the utility model; Figure 1 A connection structure schematic view of the arc-shaped air bag and the adjusting component.

[0020] Figure 4 for Figure 3 The diagram shows the structure of the adjustment component.

[0021] Figure 5 for Figure 3 The diagram shows the structure of the arc-shaped airbag.

[0022] Figure 6 for Figure 1 The diagram shows the structure of the threaded pipe cap.

[0023] The following are the labels in the diagram: 1. Centrifuge tube body; 1a. Annular groove; 1b. Cut groove; 1c. Threaded part; 11. Threaded tube opening; 2. Threaded tube cap; 21. Connecting piece; 22. Collar; 3. Arc-shaped air bladder; 4. Adjusting component; 41. Upper abutment ring; 42. Middle connecting plate; 43. Threaded ring; 431. Rotating ring. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0025] The specific implementation of this utility model will be described in detail below with reference to specific embodiments.

[0026] Please see Figures 1 to 6 This utility model provides a protein purification mixing container, which includes a centrifuge tube 1, a threaded cap 2, an arc-shaped air bladder 3, and an adjustment component 4.

[0027] In the embodiments of this utility model, please refer to Figures 1 to 6 The centrifuge tube 1 is provided with a threaded tube cap 2. Specifically, the top opening of the centrifuge tube 1 is a threaded tube opening 11 structure, and a connecting piece 21 is fixedly installed on the threaded tube cap 2. A collar 22 is fixedly installed on the other end of the connecting piece 21. The collar 22 is fitted into the annular groove opened in the centrifuge tube 1 and is rotatably connected to the annular groove.

[0028] It should be noted that after the filler and buffer solution are placed into the centrifuge tube 1, the threaded cap 2 is then placed over the threaded opening 11 on the centrifuge tube 1 and the threads are tightened.

[0029] In the embodiments of this utility model, please refer to Figures 1 to 6 Figures 1 to 6The centrifuge tube 1 has an annular groove 1a and multiple annularly distributed grooves 1b on the tube body near the bottom. The grooves 1b are located above the annular groove 1a and are connected to the annular groove 1a. The centrifuge tube 1 above the grooves 1b has an integrally formed threaded part 1c. Multiple annularly distributed arc-shaped airbags 3 are installed in the annular groove 1a of the centrifuge tube 1. The centrifuge tube 1 is also equipped with an adjustment component 4, which drives the arc-shaped airbags 3 to compress and expand.

[0030] The adjusting component 4 includes an upper abutment ring 41, which is sleeved on the annular groove 1a of the centrifuge tube 1 and slidably connected to the annular groove 1a. Multiple vertically arranged and annularly distributed connecting plates 42 are fixedly installed on the upper abutment ring 41. The connecting plates 42 are inserted into the corresponding cut grooves 1b and slidably connected to the cut grooves 1b. A threaded ring 43 is mounted on the top of the connecting plate 42. A rotating ring 431 is rotatably connected to the bottom of the threaded ring 43. The rotating ring 431 is fixedly connected to the top of the connecting plate 42. The threaded ring 43 is sleeved on the centrifuge tube 1. The lower surface of the arc-shaped airbag 3 is fixedly fitted on the upper abutment ring 41, and the upper surface of the arc-shaped airbag 3 is fixedly connected to the inner top wall of the annular groove 1a. The outer diameter of the arc-shaped airbag 3 is the same as the outer diameter of the centrifuge tube 1.

[0031] It should be noted that when using a vortex mixer to mix the solution in centrifuge tube 1, centrifuge tube 1 is inserted into the test tube holder hole of the vortex mixer, and then the threaded ring 43 is pulled up. At this time, the connecting plate 42 drives the upper abutment ring 41 to slide up along the annular groove 1a opened in the centrifuge tube 1, thereby the arc-shaped air bag 3 is compressed upward. At this time, the arc-shaped air bag 3 is compressed and expanded. Since the inner wall of the arc-shaped air bag 3 is in contact with the groove wall of the annular groove 1a, the arc-shaped air bag 3 can expand and extend radially outward. Then, the threaded ring 43 is rotated along the thread. Simply tighten part 1c to quickly fill the gap between the centrifuge tube 1 and the tube hole of the test tube rack, significantly increasing the friction between them and achieving a stable lock of the centrifuge tube 1 on the test tube rack. This effectively avoids the risk of the centrifuge tube 1 shaking, shifting, or jumping out during the high-speed oscillation of the vortex oscillator, ensuring the safe and orderly conduct of the experiment. At the same time, the centrifuge tube 1 is fixed by its own structural design, eliminating the need for additional auxiliary fixing parts such as spring clips, simplifying the experimental preparation process, reducing operation steps and time costs, and improving experimental efficiency.

[0032] It should also be noted that after mixing is complete, the reverse-rotating threaded ring 43 is pressed down, thereby the connecting plate 42 drives the upper abutment ring 41 to slide down along the annular groove 1a opened in the centrifuge tube 1. At this time, the arc-shaped airbag 3 elastically recovers, and then the centrifuge tube 1 can be removed.

[0033] Furthermore, the arc-shaped airbag 3 can expand to different degrees through the adjustment component 4, which can adapt to the tube holes of the test tube rack with small size differences, meet the usage needs of various experimental scenarios, and reduce the investment cost of experimental equipment.

[0034] In addition, medical-grade silicone rubber or fluororubber, which is resistant to chemical corrosion, aging, and has excellent elastic recovery properties, is selected as the material for the arc-shaped airbag 3 to improve its weather resistance and service life. Furthermore, an anti-fouling coating can be applied to the surface of the arc-shaped airbag 3 to reduce impurity residue and chemical corrosion.

[0035] The circuits and controls involved in this utility model are all existing technologies, and will not be described in detail here.

[0036] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A protein purification mixing container, comprising a centrifuge tube (1) and a threaded cap (2) disposed on the centrifuge tube (1), characterized in that: The centrifuge tube (1) has an annular groove (1a) and multiple annularly distributed cutting grooves (1b) on the tube body near the bottom. The cutting grooves (1b) are located above the annular grooves (1a) and are interconnected with the annular grooves (1a). The centrifuge tube (1) above the cutting grooves (1b) is provided with an integrally formed threaded part (1c). Multiple circularly distributed arc-shaped airbags (3) are installed in the annular groove (1a) of the centrifuge tube (1), and an adjustment component (4) is also installed on the centrifuge tube (1), which drives the arc-shaped airbags (3) to compress and expand.

2. The protein purification mixing container according to claim 1, characterized in that, The adjusting component (4) includes an upper abutment ring (41), which is sleeved on the annular groove (1a) opened in the centrifuge tube (1) and slidably connected to the annular groove (1a). Multiple vertically arranged and annularly distributed connecting plates (42) are fixedly installed on the upper abutment ring (41). The connecting plates (42) are inserted into the corresponding cut grooves (1b) and slidably connected to the cut grooves (1b). A threaded ring (43) is mounted on the top of the connecting plate (42).

3. The protein purification mixing container according to claim 2, characterized in that, The bottom of the threaded ring (43) is fitted with a rotating ring (431) that is rotatably connected, and the rotating ring (431) is fixedly connected to the top of the connecting plate (42).

4. The protein purification mixing container according to claim 3, characterized in that, The threaded ring (43) is fitted onto the centrifuge tube (1).

5. The protein purification mixing container according to claim 2, characterized in that, The lower surface of the arc-shaped airbag (3) is fixedly fitted onto the upper abutment ring (41), and the upper surface of the arc-shaped airbag (3) is fixedly connected to the inner top wall of the annular groove (1a). The outer diameter of the arc-shaped airbag (3) is the same as the outer diameter of the centrifuge tube (1).

6. The protein purification mixing container according to claim 1, characterized in that, The centrifuge tube (1) has a threaded tube opening (11) at the top.

7. The protein purification mixing container according to claim 6, characterized in that, A mating piece (21) is fixedly installed on the threaded tube cap (2), and a collar (22) is fixedly installed on the other end of the mating piece (21). The collar (22) is fitted into the annular groove opened in the centrifuge tube body (1) and is rotatably connected to the annular groove.

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