Portable real-time fluorescent PCR amplification equipment

Abstract

The utility model discloses portable real-time fluorescent PCR (Polymerase Chain Reaction) amplification equipment which comprises a real-time fluorescent PCR detection shell and a wire coiling mechanism, and an interface board is arranged on the rear side surface of the real-time fluorescent PCR detection shell; the wire winding mechanism comprises wire winding plates, rotating columns, rotating blocks, telescopic rods and springs, the rotating columns are rotationally connected to the rear side face of the real-time fluorescent PCR detection shell, the wire winding plates are arranged at the rear ends of the rotating columns, the two rotating blocks are distributed left and right, the rotating block on the left side is rotationally connected with a rotating frame on the inner wall of the real-time fluorescent PCR detection shell, the rotating columns are rotationally connected with rotating blocks on the right side, and the telescopic rods are connected with the telescopic rods. According to the portable real-time fluorescent PCR amplification equipment, support is provided for coiling of a connecting line of the real-time fluorescent PCR amplification equipment, the coiled connecting line is rapidly limited, the situation that the connecting line swings willfully in the process that a person carries the real-time fluorescent PCR amplification equipment to move is avoided, and the real-time fluorescent PCR amplification equipment is more convenient to carry and move.

Description

TECHNICAL FIELD

[0001] The utility model relates to real -time fluorescent PCR technical field, concretely is a kind of portable real -time fluorescent PCR amplification equipment. BACKGROUND

[0002] Real-time fluorescent PCR is a kind of fluorescence group in PCR reaction system, utilize fluorescence signal accumulation real-time monitoring entire PCR process, finally through standard curve to the initial template quantitative analysis method, fluorescence quantitative PCR has high accuracy, high sensitivity, strong specificity and other advantages, it has been widely used in molecular biology research and medical research etc.Field;In prior art, grant publication No.CN 118546774 B proposes a kind of real-time fluorescent PCR analyzer and its detection method, including display screen module, control mainboard, amplification reaction module and communication mainboard, control mainboard is installed with embedded software, corresponding detection data can be directly displayed in display screen, need not to be connected with computer for analysis operation, the display screen in the equipment has the function of turning over, it is convenient for different body shape of experimental personnel to use, and after turning over, screen and prevent dust etc.Falling into the detection hole of amplification module, reduce the land space of overall equipment, facilitate the carrying movement of equipment, but although the equipment does not need to connect external computer in use process, still need to be connected with external power supply or external joint use equipment, when carrying equipment moves, connecting line is prone to swing, it is inconvenient to carry. UTILITARIAN CONTENT

[0003] The utility model solves the technical problems of overcoming the defects of the prior art, provides a portable real-time fluorescent PCR amplification equipment, provides support for the coiling of the connecting lines of the real-time fluorescent PCR amplification equipment, limits the connecting lines that have been coiled, prevents the connecting lines from swinging freely during the movement of the real-time fluorescent PCR amplification equipment, makes the movement of the real-time fluorescent PCR amplification equipment more convenient, and effectively solves the problems in the background art.

[0004] To achieve the above object, the utility model provides the following technical scheme: a kind of portable real-time fluorescent PCR amplification equipment, including real-time fluorescent PCR detection shell, the rear side of the real-time fluorescent PCR detection shell is equipped with interface board, further including wire coiling mechanism;

[0005] The disc line mechanism comprises a winding plate, a rotating column, a rotating block, an extension rod and a spring, the rotating column is rotationally connected to the rear side of the real-time fluorescent PCR detection shell respectively, the rear end of the rotating column is provided with the winding plate, the rotating block is distributed left and right, the left rotating block is rotationally connected with the rotating frame of the inner wall of the real-time fluorescent PCR detection shell, the rotating column is rotationally connected with the right rotating block, the spring and the extension rod are arranged between the two rotating blocks respectively, the spring is movably sleeved on the outer arc surface of the extension rod, the spring provides support for the winding of the connecting line of the real-time fluorescent PCR amplification equipment, the connecting line which is wound is quickly limited, the connecting line is prevented from swinging freely during the movement of the real-time fluorescent PCR amplification equipment, and the movement of the real-time fluorescent PCR amplification equipment is more convenient.

[0006] Further, the disc line mechanism further comprises a connecting plate, the connecting plate is rotationally connected between the rotating grooves at the front ends of the outer arc surfaces of the two rotating columns, and the right rotating block is rotationally connected with the cylindrical column in the middle of the connecting plate, so that the two rotating columns rotate synchronously.

[0007] Further, the disc line mechanism further comprises a gear and a rack plate, the gear is arranged at the front end of the rotating column, a guide support is arranged at the right end of the connecting plate, a sliding column is vertically and slidably connected in the guide support, and the rack plate is arranged at the upper end and the lower end of the sliding column; the rack plate is meshingly connected with the adjacent gear, so that the two winding plates rotate synchronously, and the connecting line can be conveniently taken off from the winding plate.

[0008] Further, the disc line mechanism further comprises a torsional spring, the torsional spring is movably sleeved on the outer arc surface of the rotating column, the front end of the torsional spring is fixedly connected with the adjacent gear, and the rear end of the torsional spring is fixedly connected with the connecting plate, so as to limit the position of the winding plate.

[0009] Further, the winding plate is a hollow plate, the left end of the winding plate is of an open structure, the inside of the winding plate is horizontally and slidably connected with an extension winding plate, a screw hole is formed in the right end of the extension winding plate, and a screw is threadedly connected in the screw hole; the screw passes through the insertion hole in the surface of the winding plate, so that the length of the disc line component is increased, and more connecting lines can be wound on the disc line component.

[0010] Further, the left end of the extension winding plate is provided with a limiting block, so as to limit the connecting line from being separated from the winding component.

[0011] Further, the rear side of the real-time fluorescent PCR detection shell is provided with a rubber pad, the rubber pad corresponds to the front and rear positions of the winding plate, and provides elastic support for the limiting of the connecting line.

[0012] Compared with the prior art, the portable real-time fluorescent PCR amplification equipment has the following advantages:

[0013] In order to provide support for the coiling of the connecting line of the real-time fluorescent PCR amplification equipment, limit the completed connecting line, avoid the swinging of the connecting line during the carrying of the real-time fluorescent PCR amplification equipment, and make the carrying of the real-time fluorescent PCR amplification equipment more convenient. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a structural schematic view of the utility model;

[0015] Figure 2 It is a structural schematic view of the utility model from the top of the wire winding mechanism;

[0016] Figure 3 It is a structural schematic view of the utility model from the top of the wire winding mechanism;

[0017] Figure 4 It is a structural schematic view of the utility model from the top of the wire winding mechanism.

[0018] In the figure: 1 real-time fluorescent PCR detection shell, 2 interface plate, 3 wire winding mechanism, 31 winding plate, 32 rotating column, 33 rotating block, 34 telescopic rod, 35 spring, 36 connecting plate, 37 gear, 38 rack plate, 39 torsional spring, 4 telescopic winding plate, 5 bolt, 6 limiting block, 7 rubber pad. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0020] Please refer to Figures 1-4 The embodiment provides a technical scheme: a portable real-time fluorescent PCR amplification equipment, which comprises a real-time fluorescent PCR detection shell 1, real-time fluorescent PCR detection components in the real-time fluorescent PCR detection shell 1, the real-time fluorescent PCR detection components adopt a common real-time fluorescent PCR detector in the prior art, mainly composed of a thermal cycling device, an optical detection system and a fluorescent detection channel, the thermal cycling device controls the temperature change of PCR reaction, provides appropriate temperature for the amplification of samples, the optical detection system is usually composed of a laser, a filter, a photodiode and the like, and is used for realizing the change of the fluorescent signal in the PCR reaction process, the fluorescent detection channel is used for simultaneously detecting multiple target sequences or multiple fluorescent labels, the rear surface of the real-time fluorescent PCR detection shell 1 is provided with an interface plate 2, the connecting line is conveniently connected with the real-time fluorescent PCR amplification equipment, and the wire winding mechanism 3 is further included.

[0021] The disc winding mechanism 3 comprises a winding plate 31, a rotating column 32, a rotating block 33, an extension rod 34 and a spring 35, the rotating column 32 is rotatably connected to the rear side of the real-time fluorescent PCR detection shell 1 respectively, the rear end of the rotating column 32 is provided with the winding plate 31, the rotating block 33 is distributed left and right, the left rotating block 33 is rotatably connected with the rotating frame of the inner wall of the real-time fluorescent PCR detection shell 1, the rotating column 32 is rotatably connected with the right rotating block 33, the spring 35 and the extension rod 34 are arranged between the two rotating blocks 33, the spring 35 is movably sleeved on the outer arc surface of the extension rod 34, the disc winding mechanism 3 further comprises a connecting plate 36, the connecting plate 36 is rotatably connected between the rotating grooves of the outer arc surface front ends of the two rotating columns 32, the right rotating block 33 is rotatably connected with the cylindrical body in the middle of the connecting plate 36, when it is needed to transfer the real-time fluorescent PCR amplification equipment, the connecting line is disconnected with the external equipment, then the connecting line is wound on the two winding plates 31 distributed upwards and downwards, after winding, the winding plate 31 is pressed, the rotating column 32 is driven to move forward, under the connection of the connecting plate 36, the right rotating block 33 is driven to move forward, because the position of the left rotating block 33 is unchanged, the change of the interval between the two rotating blocks 33 will drive the spring 35 and the extension rod 34 to contract, when the right rotating block 33 moves to the front side of the left rotating block 33, the extension rod 34 is in a gradually forward inclined state from left to right, under the elastic force of the spring 35, the extension rod 34 is stretched and contracted, the right rotating block 33 is driven to move forward, the position of the winding plate 31 is limited, the connecting line is pressed on the rear side of the real-time fluorescent PCR detection shell 1 by the winding plate 31, the connecting line is prevented from swinging randomly during the carrying and transferring process of the real-time fluorescent PCR amplification equipment, the carrying of the real-time fluorescent PCR amplification equipment is more convenient, the disc winding mechanism 3 further comprises a gear 37 and a rack plate 38, the gear 37 is arranged at the front end of the rotating column 32, a guide support is arranged at the right end of the connecting plate 36, a slide column is vertically and slidably connected in the guide support, the rack plate 38 is arranged at the upper and lower ends of the slide column, the rack plate 38 is rotatably connected with the adjacent gear 37, the disc winding mechanism 3 further comprises a torsional spring 39, the torsional spring 39 is movably sleeved on the outer arc surface of the rotating column 32, the front end of the torsional spring 39 is fixedly connected with the adjacent gear 37, the rear end of the torsional spring 39 is fixedly connected with the connecting plate 36, under the torsional force of the torsional spring 39, the winding plate 31 is kept in a horizontal state, when it is needed to take the connecting line off the winding plate 31, one of the winding plates 31 is rotated, under the connection of the rotating column 32, the corresponding gear 37 is driven to rotate, under the meshing connection of the gear 37 and the rack plate 38, the rack plate 38 is driven to move vertically, under the connection of the slide column between the two rack plates 38, the two rack plates 38 are synchronously moved vertically, the two winding plates 31 are synchronously rotated, because the two rack plates 38 are left and right symmetrical, the two rack plates 38 contact the adjacent gears 37 at different positions, so under the condition that the moving directions of the two rack plates 38 are same, the rotating directions of the two gears 37 are opposite, the relative rotation of the two winding plates 31 is facilitated, the connecting line is conveniently taken off the winding plate 31, the winding plate 31 is a hollow plate,The left end of the winding plate 31 is an open structure, the inside of the winding plate 31 is horizontally and slidably connected with the telescopic winding plate 4, the screw hole in the right end of the telescopic winding plate 4 is threadedly connected with the bolt 5, the bolt 5 penetrates the jack on the surface of the winding plate 31 respectively, the length of the telescopic winding plate 4 from the inside of the winding plate 31 is changed by the different plug-in positions of the bolt 5 and the winding plate 31, so that the winding part can provide winding support for different numbers of connecting lines, the left end of the telescopic winding plate 4 is provided with the limiting block 6, the connecting line is prevented from falling off from the winding part, the rear side of the real-time fluorescent PCR detection shell 1 is provided with the rubber pad 7, the rubber pad 7 corresponds to the front and rear positions of the winding plate 31, after the connecting line is fixed by the winding plate 31, the friction force between the connecting line and the winding plate 31 is increased by the acting force generated by the deformation of the rubber pad 7.

[0022] The working principle of the portable real-time fluorescent PCR amplification device is as follows: in use, the sample with a fluorescent group is placed into the inside of the real-time fluorescent PCR detection shell 1, the thermal cycle device in the inside of the real-time fluorescent PCR detection shell 1 provides appropriate temperature for the amplification of the sample, the requirement of the PCR reaction is met, in the process of the sample amplification, the optical detection system in the inside of the real-time fluorescent PCR detection shell 1 is used for monitoring the change of the fluorescent signal in the PCR reaction process in real time, the amplification of the sample is monitored in real time according to the change of the fluorescent signal, when the real-time fluorescent PCR amplification device needs to be transferred, the connecting line is disconnected with the external device, then the connecting line is wound on the two winding plates 31 distributed upwards and downwards, after winding is completed, the winding plate 31 is pressed, the rotating column 32 is driven to move forward, under the connection of the connecting plate 36, the rotating block 33 on the right is driven to move forward, because the position of the rotating block 33 on the left is unchanged, the change of the distance between the two rotating blocks 33 will drive the spring 35 and the telescopic rod 34 to contract, when the rotating block 33 on the right moves to the front side of the rotating block 33 on the left, the telescopic rod 34 is in a gradually forward tilting state from left to right, the telescopic rod 34 is telescoped under the elastic force of the spring 35, the rotating block 33 on the right is driven to move forward, the position of the winding plate 31 is limited, the connecting line is pressed on the surface of the rubber pad 7 by the winding plate 31, the connecting line is prevented from swinging randomly in the carrying and transferring process of the real-time fluorescent PCR amplification device, the carrying of the real-time fluorescent PCR amplification device is more convenient.

[0023] The above is only an embodiment of the utility model, and does not limit the patent range of the utility model, and equivalent structures or equivalent process transformations using the utility model specification and drawing contents, or direct or indirect application in other related technical fields are all included in the patent protection range of the utility model.

Claims

1. A portable real-time fluorescent PCR amplification device, comprising a real-time fluorescent PCR detection shell (1), the rear side of the real-time fluorescent PCR detection shell (1) is provided with an interface plate (2), characterized in that: Also include the disc line mechanism (3); The disc line mechanism (3) comprises a winding plate (31), a rotating column (32), a rotating block (33), a telescopic rod (34) and a spring (35), the rotating column (32) is rotatably connected to the rear side of the real-time fluorescent PCR detection shell (1) respectively, the rear end of the rotating column (32) is provided with the winding plate (31), the rotating block (33) has two and is distributed left and right, the left rotating block (33) is rotatably connected with the rotating frame of the inner wall of the real-time fluorescent PCR detection shell (1), the rotating column (32) is rotatably connected with the right rotating block (33), the spring (35) and the telescopic rod (34) are respectively arranged between the two rotating blocks (33), and the spring (35) is movably sleeved on the outer arc surface of the telescopic rod (34).

2. The portable real-time fluorescent PCR amplification device according to claim 1, characterized in that: The disc line mechanism (3) further comprises a connecting plate (36), the connecting plate (36) is rotatably connected between the rotating grooves of the outer arc surfaces of the two rotating columns (32), and the right rotating block (33) is rotatably connected with the cylindrical column in the middle of the connecting plate (36).

3. The portable real-time fluorescent PCR amplification device according to claim 2, characterized in that: The disc line mechanism (3) further comprises a gear (37) and a rack plate (38), the gear (37) is arranged at the front end of the rotating column (32), the guiding support at the right end of the connecting plate (36) is vertically slidably connected with a slide column, the rack plate (38) is arranged at the upper and lower ends of the slide column, and the rack plate (38) is rotatably connected with the adjacent gear (37).

4. The portable real-time fluorescent PCR amplification device according to claim 3, characterized in that: The disc line mechanism (3) further comprises a torsional spring (39), the torsional spring (39) is movably sleeved on the outer arc surface of the rotating column (32), the front end of the torsional spring (39) is fixedly connected with the adjacent gear (37), and the rear end of the torsional spring (39) is fixedly connected with the connecting plate (36).

5. The portable real-time fluorescent PCR amplification device according to claim 1, wherein: The winding plate (31) is a hollow plate, the left end of the winding plate (31) is an open structure, the inside of the winding plate (31) is horizontally slidably connected with a telescopic winding plate (4), the screw hole in the right end of the telescopic winding plate (4) is threadedly connected with a bolt (5), and the bolt (5) penetrates the jack (5) on the surface of the winding plate (31).

6. The portable real-time fluorescent PCR amplification device according to claim 5, wherein: The left end of the telescopic winding plate (4) is provided with a limiting block (6).

7. The portable real-time fluorescent PCR amplification device according to claim 1, wherein: The rear side of the real-time fluorescent PCR detection shell (1) is provided with a rubber pad (7), and the rubber pad (7) corresponds to the front and rear positions of the winding plate (31).

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