Rail type full-automatic sample pretreatment device

Abstract

The utility model discloses a rail type full-automatic sample pretreatment device which comprises a machine table, and an annular guide rail is laid on the machine table. A sample injection bin, a first cap screwing mechanism, an oscillating mechanism, a heating mechanism, a second cap screwing mechanism, a magnetic separation and reagent transfer mechanism, a centrifugal tube feeding mechanism and a nitrogen blowing mechanism are sequentially arranged around the guide rail, are connected with one another through the guide rail and work cooperatively; by adopting a rail type structure and a modularized device, all pretreatment experiment operations of pesticide and veterinary drug residue detection are automated, the instrument cost is reduced, the manpower is liberated, the error of manual operation in the experiment is reduced, the operation efficiency of pretreatment is improved, and the requirement of large-scale detection can be met.

Description

TECHNICAL FIELD

[0001] The utility model relates to a sample pretreatment device, especially to a track type full-automatic sample pretreatment device. BACKGROUND

[0002] In the field of food safety detection, the extraction and detection of pesticide and veterinary drug residues are the key links to ensure the quality and safety of agricultural products. With the rapid development of modern agriculture and animal husbandry, the widespread use of pesticides and veterinary drugs has improved yield and prevented pests and diseases, but also brought the risk of excessive residues. Due to the lack of scientific knowledge of some farmers and breeders, the phenomena of misuse of drugs, over-dose use and even addition of prohibited drugs are common, leading to the increasingly prominent problem of pesticide and veterinary drug residues in agricultural products and processed foods. These residues may cause chronic poisoning, allergic reactions and even cancer when entering the human body through the food chain, seriously threatening public health. Therefore, developing efficient and accurate pesticide and veterinary drug residue extraction and detection technology is not only an urgent need to ensure food safety, but also a necessary measure to promote sustainable agricultural development and maintain consumer confidence.

[0003] Currently, magnetic solid-phase extraction technology has been widely used in sample pretreatment for pesticide and veterinary drug residue detection and has achieved good results. This technology modifies functional groups on the surface of nanometer to micrometer magnetic beads, enabling selective adsorption of target compounds (such as pesticide and veterinary drug residues) in the sample, while removing impurities such as oil, protein, and enzymes, achieving the purpose of purification and enrichment.

[0004] Although magnetic solid-phase extraction technology has shown significant advantages in pesticide and veterinary drug residue detection, it usually requires multiple devices to complete the steps of sample weighing, liquid addition, oscillation, heating, magnetic separation, etc. in practical application, resulting in high instrument cost, large floor area, low running efficiency, long sample processing time, and difficulty in meeting the demand of large-scale detection. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a track type full-automatic sample pretreatment device that automates all pretreatment experimental operations for pesticide and veterinary drug residue detection.

[0006] Technical scheme: The track type full-automatic sample pretreatment device comprises a machine table, a ring-shaped guide rail is laid on the machine table, a sample inlet bin, a first screw cap mechanism, an oscillation mechanism, a heating mechanism, a second screw cap mechanism, a magnetic separation and reagent transfer mechanism, a centrifugal tube feeding mechanism, and a nitrogen blowing mechanism are sequentially arranged around the guide rail, and each mechanism is connected to each other through the guide rail and works cooperatively.

[0007] By the above technical scheme, the efficient cooperation of various mechanisms is realized by using the guide rail, a complete automatic sample pretreatment system is constructed, the whole process automation operation from sample injection to pretreatment completion can be realized, the detection efficiency is significantly improved, the manual operation error is reduced, and the flexibility of processing various samples and detection items is also provided, so that the high-throughput and high-precision detection requirements are met.

[0008] Preferably, the machine table includes a reagent pipeline liquid inlet, a reagent bottle placing groove, a liquid level monitor and a waste liquid bottle, which are used for storage, monitoring and waste liquid treatment of reagents. This design not only simplifies the reagent management process, but also ensures the accuracy and safety of reagent use, reduces manual intervention, and improves the automation level and reliability of the overall experiment.

[0009] Preferably, the sample loading bin includes a sample loading module, a weighing mechanism and an automatic door. The sample loading module can place different specifications of centrifugal tubes and move on the guide rail by driving motor. After the centrifugal tube is placed on the sample loading module, it is moved to the weighing mechanism for weighing. The weighing mechanism includes a cylinder fixing seat, a lifting cylinder, a lifting fixed plate, a lifting guide rod and a carrier base. The lifting cylinder is fixed on the cylinder fixing seat, the piston rod of the lifting cylinder is connected with the lifting fixed plate, the lifting fixed plate is provided with a pressure sensor, the lifting guide rod is fixed on the lifting fixed plate and connected with the carrier base, and the carrier base is used for placing the centrifugal tube. When the centrifugal tube is placed on the carrier base, the lifting cylinder drives the carrier base to rise through the lifting fixed plate, and the pressure sensor detects the weight of the centrifugal tube.

[0010] Through the cooperative work of the sample loading module and the weighing mechanism in the sample loading bin, automatic sample injection and accurate weighing of different specifications of centrifugal tubes can be realized. The sample loading module moves on the guide rail by driving motor to ensure accurate positioning and efficient transmission of the centrifugal tube. The weighing mechanism adopts the combination design of lifting cylinder, lifting fixed plate and pressure sensor, which can accurately measure the weight of the centrifugal tube and transmit the data to the control system, so as to provide accurate weight information for subsequent sample processing, improve the automation degree and accuracy of sample pretreatment, and reduce the error and time cost of manual operation.

[0011] Preferably, the first screw cap mechanism comprises a first clamping assembly A for clamping and positioning the centrifugal tube, a liquid adding mechanism for automatically adding liquid into the centrifugal tube, a first clamping assembly B, a first screw cap assembly for unscrewing or screwing the centrifugal tube cap, a first linear module for clamping the centrifugal tube cap and moving it to the position of the first clamping assembly B, and a first lifting assembly for controlling the up and down movement of the first screw cap assembly; when the object carrying module runs to the position of the first clamping assembly A, the blocking cylinder is limited, the first clamping assembly A clamps and positions the centrifugal tube, the first screw cap assembly unscrews the centrifugal tube cap, the first linear module moves the centrifugal tube cap to the position of the first clamping assembly B and waits; after the liquid adding is completed, the object carrying module moves to the position of the first clamping assembly B, and the first screw cap assembly screws the centrifugal tube cap with the centrifugal tube.

[0012] Through the automatic design of the first screw cap mechanism, the unscrewing, liquid adding and screwing operations of the centrifugal tube cap can be efficiently and accurately completed; the cooperative work of the first clamping assembly A and the first screw cap assembly ensures the accurate positioning of the centrifugal tube and the unscrewing of the cap, the liquid adding mechanism realizes the automatic addition of reagent through a high-precision pump valve system, the design of the first linear module and the first clamping assembly B enables the centrifugal tube cap to be temporarily fixed and wait for subsequent operation, and the first lifting assembly ensures the accuracy and stability of the screw cap action.

[0013] Preferably, the oscillation mechanism comprises a second clamping assembly, a second linear module and an oscillator; the second clamping assembly is used to grab the centrifugal tube on the object carrying module and transfer it to the oscillator through the second linear module; the oscillator is used to oscillate the sample in the centrifugal tube; after the oscillation is completed, the second clamping assembly transfers the centrifugal tube back to the object carrying module through the second linear module.

[0014] Through the automatic design of the oscillation mechanism, the grabbing, transferring and oscillation processing of the centrifugal tube can be efficiently and accurately completed; the cooperative work of the second clamping assembly and the second linear module ensures the stable grabbing and accurate transferring of the centrifugal tube, the oscillator realizes the full mixing or reaction of the sample through high-frequency oscillation technology, which improves the uniformity and consistency of sample processing; after the oscillation is completed, the centrifugal tube is automatically transferred back to the object carrying module, realizing the full automation of sample processing and significantly improving the operation efficiency.

[0015] Preferably, the heating mechanism comprises a third clamping assembly, a third linear module and a heater; the third clamping assembly is used to grab the centrifugal tube on the object carrying module and transfer it to the heater through the third linear module, and the heater is used to heat the sample in the centrifugal tube; after the heating is completed, the third clamping assembly transfers the centrifugal tube back to the object carrying module through the third linear module.

[0016] Through the automatic design of the heating mechanism, the centrifuge tube can be efficiently and accurately grabbed, transferred and heated; the cooperative work of the third clamping assembly and the third linear module ensures the stable grabbing and accurate transfer of the centrifuge tube, the heating instrument heats the sample through the high-precision temperature control system, and the specific experimental requirements are met; after heating, the centrifuge tube is automatically transferred back to the sample loading module, realizing the full automation of sample processing.

[0017] Preferably, the second cap screwing mechanism comprises a fourth clamping assembly for clamping and positioning the centrifuge tube, a second cap screwing assembly for unscrewing the centrifuge tube cap, a fourth linear module for moving the unscrewed centrifuge tube cap to the waste box position and discarding, and a second lifting assembly for controlling the up and down movement of the second cap screwing assembly; when the sample loading module runs to the position of the fourth clamping assembly, the blocking cylinder is limited, the fourth clamping assembly clamps and positions the centrifuge tube, the second cap screwing assembly unscrews the centrifuge tube cap, the fourth linear module moves the centrifuge tube cap to the waste box position and discards, and the second lifting assembly controls the lifting of the second cap screwing assembly to complete the cap screwing operation.

[0018] Through the automatic design of the second cap screwing mechanism, the positioning, cap screwing and waste cap discarding operations of the centrifuge tube can be efficiently and accurately completed; the cooperative work of the fourth clamping assembly and the second cap screwing assembly ensures the accurate positioning of the centrifuge tube and the unscrewing of the cap, the fourth linear module automatically moves the unscrewed centrifuge tube cap to the waste box position for discarding, and the second lifting assembly ensures the accuracy and stability of the cap screwing action.

[0019] Preferably, the magnetic separation and reagent transfer mechanism comprises a fifth clamping assembly for clamping and positioning the centrifuge tube, a fifth linear module for driving the fifth clamping assembly to move, a magnetic bead adding assembly for adding magnetic beads into the centrifuge tube, a magnetic separation assembly for magnetically separating the sample in the centrifuge tube, an adding and reagent transfer assembly for adding reagent into the centrifuge tube and transferring the separated liquid to the sample loading module on the second main track, and a centrifuge tube waste box for storing the waste centrifuge tube after magnetic separation; when the sample loading module runs to the position of the fifth clamping assembly, the blocking cylinder is limited, the fifth clamping assembly clamps the centrifuge tube, the fifth linear module moves the centrifuge tube to the magnetic bead adding position, and the magnetic bead adding assembly adds magnetic beads into the centrifuge tube; then the adding and reagent transfer assembly adds reagent into the centrifuge tube, and the magnetic separation assembly magnetically separates the sample; after the separation is completed, the adding and reagent transfer assembly transfers the separated liquid to the sample loading module on the second main track, and the fifth linear module moves the waste centrifuge tube to the centrifuge tube waste box for discarding.

[0020] By the integrated design of magnetic separation and reagent transfer mechanism, the whole process automation of magnetic bead addition, reagent distribution, magnetic separation and liquid transfer in sample processing is realized; the fifth clamping assembly and the fifth linear module ensure the precise positioning and efficient transfer of the centrifugal tube, the magnetic bead addition assembly and the reagent transfer assembly realize the accurate addition of reagent through the high-precision control system, the magnetic separation assembly separates the target material quickly by using the magnetic field, and the separated liquid is automatically transferred to the second main track, and the waste centrifugal tube is automatically discarded into the waste box; this design not only greatly improves the efficiency and accuracy of sample processing, but also reduces reagent waste and manual operation, and is especially suitable for biological sample experimental scenes that require high throughput and high precision.

[0021] Preferably, the centrifugal tube feeding mechanism includes a sixth clamping assembly for clamping the centrifugal tube, a sixth linear module for driving the sixth clamping assembly to move, and a centrifugal tube feeding assembly; when the carrier module runs to the position of the sixth clamping assembly, the blocking cylinder is limited, the sixth clamping assembly clamps the centrifugal tube provided by the centrifugal tube feeding assembly, and the centrifugal tube is transferred to the carrier module by the sixth linear module.

[0022] Through the automatic design of the centrifugal tube feeding mechanism, the precise feeding and efficient transfer of the empty centrifugal tube are realized; the cooperative work of the sixth clamping assembly and the sixth linear module ensures the stable grabbing and accurate placement of the centrifugal tube, and the centrifugal tube feeding assembly can continuously provide empty centrifugal tubes to meet the demand of high-throughput sample processing; this design significantly reduces manual intervention, improves feeding efficiency, and ensures the continuity and stability of the sample processing process, and is especially suitable for experimental scenes that require rapid and continuous operation.

[0023] Preferably, the nitrogen blowing mechanism includes a seventh clamping assembly for clamping the centrifugal tube, a seventh linear module for driving the seventh clamping assembly to move, and a nitrogen blowing instrument for nitrogen blowing treatment of the sample in the centrifugal tube; when the carrier module runs to the position of the seventh clamping assembly, the seventh clamping assembly clamps the centrifugal tube in the carrier module, and the centrifugal tube is transferred to the nitrogen blowing instrument for nitrogen blowing treatment by the seventh linear module; after nitrogen blowing is completed, the seventh linear module transfers the centrifugal tube back to the carrier module.

[0024] Through the automatic design of the nitrogen blowing mechanism, the precise grabbing, transfer and nitrogen blowing treatment of the centrifugal tube are realized; the cooperative work of the seventh clamping assembly and the seventh linear module ensures the stable grabbing and accurate transfer of the centrifugal tube, and the nitrogen blowing instrument performs nitrogen blowing treatment on the sample through a high-precision airflow control system, effectively removing the solvent or concentrating the sample; this design significantly improves the efficiency and consistency of nitrogen blowing operation, reduces manual intervention and operation errors, and is especially suitable for experimental scenes that require high-precision solvent removal or sample concentration, further enhancing the automation level of sample pretreatment.

[0025] Beneficial effects: compared with the prior art, the utility model has the advantages that: 1, reduce the instrument cost, liberate the manpower, reduce the error of artificial operation in experiment, improve the running efficiency of pretreatment, can satisfy the demand of large-scale detection;2, through the modular mechanism, the operation step is simplified obviously, and different samples, different detection projects carry out pretreatment simultaneously, improve the processing efficiency;3, in the nitrogen blowing mechanism, the nitrogen blowing needle head is driven under the sixth lifting assembly and accurately controls the height, ensures the nitrogen blowing effect, the second heater is combined with the temperature control device, realizes the accurate control of heating temperature, improves the accuracy and consistency of sample processing. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 It is the structural schematic diagram of the utility model;

[0027] Figure 2 It is the structural schematic diagram of the weighing mechanism of the utility model;

[0028] Figure 3 It is the structural schematic diagram of the first cap screwing mechanism of the utility model;

[0029] Figure 4 It is the structural schematic diagram of the oscillation mechanism of the utility model;

[0030] Figure 5 It is the structural schematic diagram of the heating mechanism of the utility model;

[0031] Figure 6 It is the structural schematic diagram of the second cap screwing mechanism of the utility model;

[0032] Figure 7 It is the structural schematic diagram of the magnetic separation and reagent transfer mechanism of the utility model;

[0033] Figure 8 It is the structural schematic diagram of the centrifugal tube feeding mechanism of the utility model;

[0034] Figure 9 It is the structural schematic diagram of the nitrogen blowing mechanism of the utility model. DETAILED DESCRIPTION

[0035] The technical scheme of the utility model is further explained in connection with the drawings.

[0036] As Figure 1As shown, the track type full-automatic sample pretreatment device comprises a machine table (1), a ring-shaped guide rail is laid on the machine table, the guide rail adopts high-precision transmission design, and the guide rail ensures that the object carrying module can be moved stably and accurately; the sample inlet bin (2), the first screw cap mechanism (3), the oscillation mechanism (4), the heating mechanism (5), the second screw cap mechanism (6), the magnetic separation and reagent transfer mechanism (7), the centrifugal tube feeding mechanism (8) and the nitrogen blowing mechanism (9) are sequentially arranged around the guide rail; the mechanisms are connected with each other through the guide rail and work cooperatively to realize full-automatic pretreatment of the sample; the machine table (1) is further provided with a reagent pipeline liquid inlet, a reagent bottle placing groove, a liquid level monitor and a waste liquid bottle for storing, monitoring and treating the reagent and waste liquid; the liquid level monitor can monitor the liquid level in the reagent bottle in real time to ensure the accuracy of reagent addition.

[0037] The sample inlet bin (2) is the first station of the sample entering the pretreatment device and comprises an object carrying module, a weighing mechanism and an automatic door; the object carrying module adopts modular design and can place centrifugal tubes (such as 50mL and 15mL) of different specifications and is moved on the guide rail through a driving motor; the movement of the object carrying module is controlled by a high-precision motor to ensure accurate positioning; after the centrifugal tube is placed on the object carrying module, the object carrying module is moved along the guide rail to the weighing mechanism for weighing; the weighing mechanism comprises a cylinder fixing seat (10), a jacking cylinder (11), a jacking fixed plate (12), a jacking guide rod (13) and a carrier base (14); the jacking cylinder (11) is fixed on the cylinder fixing seat (10), the piston rod thereof is connected with the jacking fixed plate (12), the jacking fixed plate (12) is provided with a high-precision pressure sensor, the jacking guide rod (13) is fixed on the jacking fixed plate (12) and connected with the carrier base (14); when the centrifugal tube is placed on the carrier base (14), the jacking cylinder (11) drives the carrier base (14) to rise through the jacking fixed plate (12), the weight of the centrifugal tube is detected by the pressure sensor, and data is transmitted to the control system to ensure the accuracy of the sample weight.

[0038] The first screw cap mechanism (3) is used for unscrewing the centrifuge tube cap and completing the liquid adding operation, comprising a first clamping assembly A (16), a liquid adding mechanism (17), a first clamping assembly B (18), a first screw cap assembly (19), a first linear module (20) and a first lifting assembly (21); the first clamping assembly A (16) is designed with high-precision clamping jaws, which can firmly clamp the centrifuge tube and accurately position it; when the carrier module runs to the position of the first clamping assembly A (16), the blocking cylinder is limited, the first clamping assembly A (16) clamps the centrifuge tube to position it, and the first screw cap assembly (19) unscrews the centrifuge tube cap through a rotating action; the first linear module (20) uses high-precision linear guides to move the centrifuge tube cap to the position of the first clamping assembly B (18) for waiting; the carrier module runs to the position of the liquid adding mechanism (17), the liquid adding mechanism (17) adds reagent into the centrifuge tube through a high-precision pump valve system, after the liquid adding is completed, the carrier module moves to the position of the first clamping assembly B (18), and the first screw cap assembly (19) screws the centrifuge tube cap with the centrifuge tube; the first lifting assembly (21) is driven by a motor to control the up and down movement of the first screw cap assembly (19), ensuring the accuracy of the screw cap operation.

[0039] The oscillation mechanism (4) is used for oscillating the sample to mix or react it, comprising a second clamping assembly (22), a second linear module (23) and an oscillator (24); the second clamping assembly (22) is designed with high-precision clamping jaws, which can firmly grasp the centrifuge tube; when the carrier module runs to the position of the oscillation mechanism (4), the second clamping assembly (22) grasps the centrifuge tube and transfers it to the oscillator (24) through the second linear module (23); the oscillator (24) uses high-frequency oscillation technology to oscillate the sample according to preset parameters; after the oscillation is completed, the second clamping assembly (22) transfers the centrifuge tube back to the carrier module through the second linear module (23), ensuring the continuity and efficiency of the sample processing.

[0040] The heating mechanism (5) is used for heating the sample to meet specific experimental requirements, comprising a third clamping assembly (25), a third linear module (26) and a heating instrument (27); the third clamping assembly (25) is designed with high-precision clamping jaws, which can firmly grasp the centrifuge tube; when the carrier module runs to the position of the heating mechanism (5), the third clamping assembly (25) grasps the centrifuge tube and transfers it to the heating instrument (27) through the third linear module (26); the heating instrument (27) uses a high-precision temperature control system to heat the sample according to the preset temperature. After the heating is completed, the third clamping assembly (25) transfers the centrifuge tube back to the carrier module through the third linear module (26), ensuring the accuracy and consistency of the sample processing.

[0041] The second cap opening mechanism (6) is used for opening the centrifuge tube cap and discarding the waste cap, comprising a fourth clamping assembly (28), a second cap opening assembly (29), a fourth linear module (30) and a second lifting assembly (31); the fourth clamping assembly (28) is designed with high-precision clamping jaws, which can firmly clamp the centrifuge tube and accurately position it; when the object carrying module runs to the position of the fourth clamping assembly (28), the blocking cylinder is limited, the fourth clamping assembly (28) clamps the centrifuge tube to position it, and the second cap opening assembly (29) opens the centrifuge tube cap through rotating action; the fourth linear module (30) uses high-precision linear guide rails to move the centrifuge tube cap to the waste box (32) position for discarding; the second lifting assembly (31) is driven by a motor to control the up-and-down action of the second cap opening assembly (29), ensuring the accuracy of the cap opening operation.

[0042] The magnetic separation and reagent transfer mechanism (7) is used for magnetic bead separation and reagent transfer operation of the sample, comprising a fifth clamping assembly (33), a fifth linear module (34), a magnetic bead adding assembly (35), a magnetic separation assembly (36), an adding and reagent transfer assembly (37) and a centrifuge tube waste box (38); the fifth clamping assembly (33) is designed with high-precision clamping jaws, which can firmly clamp the centrifuge tube; when the object carrying module runs to the position of the fifth clamping assembly (33), the blocking cylinder is limited, the fifth clamping assembly (33) clamps the centrifuge tube, the fifth linear module (34) moves the centrifuge tube to the magnetic bead adding position, the magnetic bead adding assembly (35) adds magnetic beads into the centrifuge tube through a high-precision pump valve system; then, the adding and reagent transfer assembly (37) adds reagent into the centrifuge tube, the magnetic separation assembly (36) performs magnetic separation on the sample through magnetic field action; after the separation is completed, the adding and reagent transfer assembly (37) transfers the separated liquid to the object carrying module on the second main track, the fifth linear module (34) moves the waste centrifuge tube to the centrifuge tube waste box (38) for discarding, ensuring the efficiency and environmental protection of sample processing.

[0043] The centrifuge tube feeding mechanism (8) is used for automatically feeding empty centrifuge tubes, comprising a sixth clamping assembly (39), a sixth linear module (40) and a centrifuge tube feeding assembly (41); the sixth clamping assembly (39) is designed with high-precision clamping jaws, which can firmly clamp the centrifuge tube; when the object carrying module runs to the position of the sixth clamping assembly (39), the blocking cylinder is limited, the sixth clamping assembly (39) clamps the centrifuge tube provided by the centrifuge tube feeding assembly (41) and transfers the centrifuge tube to the object carrying module through the sixth linear module (40), ensuring the continuity and efficiency of sample processing.

[0044] The nitrogen blowing mechanism (9) is used for nitrogen blowing treatment of the sample to remove the solvent or concentrate the sample, and comprises a seventh clamping assembly (42), a seventh linear module (43) and a nitrogen blowing instrument (44); the seventh clamping assembly (42) is designed in a high-precision clamping jaw and can firmly clamp the centrifugal tube; when the sample loading module runs to the position of the seventh clamping assembly (42), the seventh clamping assembly (42) clamps the centrifugal tube in the sample loading module, and the centrifugal tube is transferred to the nitrogen blowing instrument (44) through the seventh linear module (43) to be subjected to nitrogen blowing treatment; the nitrogen blowing instrument (44) adopts a high-precision airflow control system and can perform nitrogen blowing treatment on the sample according to preset parameters. After the nitrogen blowing is completed, the seventh linear module (43) transfers the centrifugal tube back to the sample loading module, so that the accuracy and consistency of sample treatment are ensured.

[0045] The use process of the utility model is as follows:

[0046] 1. Sample injection and weighing: place the centrifugal tube containing the sample on the sample loading module of the sample injection bin (2), and move the sample loading module along the guide rail to the weighing mechanism for weighing. The weighing mechanism accurately measures the weight of the centrifugal tube through the jacking cylinder (11) and the pressure sensor, and transmits the data to the control system. After weighing is completed, the sample loading module continues to move along the guide rail to the next processing mechanism;

[0047] 2. First cap screwing and liquid adding: the sample loading module runs to the position of the first cap screwing mechanism (3), the first clamping assembly A (16) clamps the centrifugal tube to position, the first cap screwing assembly (19) unscrews the centrifugal tube cap, the first linear module (20) moves the centrifugal tube cap to the position of the first clamping assembly B (18) to wait, the sample loading module runs to the position of the liquid adding mechanism (17), the liquid adding mechanism (17) adds reagent into the centrifugal tube through the high-precision pump valve system, after liquid adding is completed, the sample loading module moves to the position of the first clamping assembly B (18), and the first cap screwing assembly (19) screws the centrifugal tube cap with the centrifugal tube;

[0048] 3. Oscillation treatment: the sample loading module runs to the position of the oscillation mechanism (4), the second clamping assembly (22) grabs the centrifugal tube and transfers it to the oscillation instrument (24) for oscillation treatment, the oscillation instrument (24) performs high-frequency oscillation on the sample according to preset parameters, so that the sample is fully mixed or reacted, and after oscillation is completed, the second clamping assembly (22) transfers the centrifugal tube back to the sample loading module;

[0049] 4. Heating treatment: the sample loading module runs to the position of the heating mechanism (5), the third clamping assembly (25) grabs the centrifugal tube and transfers it to the heating instrument (27) for heating treatment, the heating instrument (27) heats the sample according to the preset temperature to meet the requirements of specific experiments. After heating is completed, the third clamping assembly (25) transfers the centrifugal tube back to the sample loading module;

[0050] 5、Second screw cap and waste cap processing: the object carrying module runs to the second screw cap mechanism (6) position, the fourth clamping assembly (28) clamps the centrifugal tube positioning, the second screw cap assembly (29) unscrews the centrifugal tube cover, the fourth linear module (30) moves the centrifugal tube cover to the waste box (32) and discards, the second lifting assembly (31) controls the up and down action of the second screw cap assembly (29), and the accuracy of the screw cap operation is ensured.

[0051] 6、Magnetic separation and reagent transfer: the object carrying module runs to the magnetic separation and reagent transfer mechanism (7) position, the fifth clamping assembly (33) clamps the centrifugal tube, the magnetic bead adding assembly (35) adds magnetic beads into the centrifugal tube, the adding and reagent transfer assembly (37) adds reagents, the magnetic separation assembly (36) performs magnetic separation on the sample through the action of a magnetic field, after separation, the adding and reagent transfer assembly (37) transfers the separated liquid to the object carrying module on the second main track, and the fifth linear module (34) moves the waste centrifugal tube to the centrifugal tube waste box (38) and discards.

[0052] 7、Centrifugal tube feeding: the object carrying module runs to the centrifugal tube feeding mechanism (8) position, the sixth clamping assembly (39) clamps the centrifugal tube feeding assembly (41) provided centrifugal tube, and transfers it to the object carrying module, so that the continuity and efficiency of sample processing are ensured.

[0053] 8、Nitrogen blowing treatment: the object carrying module runs to the nitrogen blowing mechanism (9) position, the seventh clamping assembly (42) clamps the centrifugal tube and transfers it to the nitrogen blowing instrument (44) for nitrogen blowing treatment, the nitrogen blowing instrument (44) performs nitrogen blowing on the sample according to preset parameters, so as to remove solvent or concentrate the sample, after nitrogen blowing, the seventh linear module (43) transfers the centrifugal tube back to the object carrying module.

[0054] Through the above steps, the utility model realizes full automation of sample pretreatment from sample feeding, weighing, screw cap, liquid adding, oscillation, heating, magnetic separation, reagent transfer, centrifugal tube feeding to nitrogen blowing, significantly improves the efficiency and accuracy of sample processing, and is suitable for various scenes such as market supervision, production enterprise quality supervision, food safety risk spot check and the like.

Claims

1. A track-based fully automated sample pretreatment device, characterized in that, The machine table (1) is provided with a ring-shaped guide rail, a sample feeding bin (2), a first cap screwing mechanism (3), a shaking mechanism (4), a heating mechanism (5), a second cap screwing mechanism (6), a magnetic separation and reagent transferring mechanism (7), a centrifugal tube feeding mechanism (8) and a nitrogen blowing mechanism (9) arranged around the guide rail in sequence.

2. The sample pretreatment device according to claim 1, characterized by The machine table (1) comprises a reagent pipeline liquid inlet, a reagent bottle placing groove, a liquid level monitor and a waste liquid bottle, which are used for storing, monitoring and treating waste liquid of the reagent.

3. The sample pretreatment device according to claim 1, characterized by The sample feeding bin (2) comprises a carrier module, a weighing mechanism and an automatic door, the carrier module can place centrifugal tubes of different specifications and is driven to move on the guide rail by a driving motor, after the centrifugal tube is placed on the carrier module, it is moved to the weighing mechanism to be weighed; the weighing mechanism comprises a cylinder fixing seat (10), a jacking cylinder (11), a jacking fixed plate (12), a jacking guide rod (13) and a carrier base (14); the jacking cylinder (11) is fixed on the cylinder fixing seat (10), the piston rod of the jacking cylinder (11) is connected with the jacking fixed plate (12), the jacking fixed plate (12) is provided with a pressure sensor, the jacking guide rod (13) is fixed on the jacking fixed plate (12) and connected with the carrier base (14), the carrier base (14) is used for placing the centrifugal tube, when the centrifugal tube is placed on the carrier base (14), the jacking cylinder (11) drives the carrier base (14) to rise through the jacking fixed plate (12), and the pressure sensor detects the weight of the centrifugal tube.

4. The sample pretreatment device according to claim 1, characterized by The first cap screwing mechanism (3) comprises a first clamping assembly A (16) for clamping and positioning the centrifugal tube, a liquid adding mechanism (17) for automatically adding liquid into the centrifugal tube, a first clamping assembly B (18), a first cap screwing assembly (19) for unscrewing or screwing the cap of the centrifugal tube, a first linear module (20) for clamping the cap of the centrifugal tube and moving it to the position of the first clamping assembly B (18), and a first lifting assembly (21) for controlling the up-down action of the first cap screwing assembly; when the carrier module runs to the position of the first clamping assembly A (16), the blocking cylinder is limited, the first clamping assembly A (16) clamps and positions the centrifugal tube, the first cap screwing assembly (19) unscrews the cap of the centrifugal tube, and the first linear module (20) moves the cap of the centrifugal tube to the position of the first clamping assembly B (18) to wait; after the carrier module runs to the position of the liquid adding mechanism (17) to complete liquid adding, it moves to the position of the first clamping assembly B (18), and the first cap screwing assembly (19) screws the cap of the centrifugal tube with the centrifugal tube to complete.

5. The sample pretreatment device according to claim 1, characterized by The shaking mechanism (4) comprises a second clamping assembly (22), a second linear module (23) and a shaker (24); the second clamping assembly (22) is used for grabbing the centrifugal tube on the carrier module and transferring the centrifugal tube to the shaker (24) through the second linear module (23); the shaker (24) is used for shaking the sample in the centrifugal tube; After shaking, the second clamping assembly (22) transfers the centrifugal tube back to the carrier module through the second linear module (23).

6. The sample pretreatment device according to claim 1, characterized by The heating mechanism (5) comprises a third clamping assembly (25), a third linear module (26) and a heating instrument (27); the third clamping assembly (25) is used for grabbing the centrifugal tube on the carrier module, and the centrifugal tube is transferred to the heating instrument (27) through the third linear module (26); the heating instrument (27) is used for heating treatment of the sample in the centrifugal tube; After heating is completed, the third clamping assembly (25) transfers the centrifugal tube back to the carrier module through the third linear module (26).

7. The sample pretreatment device according to claim 1, characterized by The second cap screwing mechanism (6) comprises a fourth clamping assembly (28) for clamping and positioning the centrifugal tube, a second cap screwing assembly (29) for screwing off the cap of the centrifugal tube, a fourth linear module (30) for moving the screwed-off cap of the centrifugal tube to the position of the waste box (32) and discarding, and a second lifting assembly (31) for controlling the up-down action of the second cap screwing assembly; when the carrier module runs to the position of the fourth clamping assembly (28), the blocking cylinder is limited, the fourth clamping assembly (28) clamps and positions the centrifugal tube, the second cap screwing assembly (29) screws off the cap of the centrifugal tube, the fourth linear module (30) moves the cap of the centrifugal tube to the position of the waste box (32) and discards, and the second lifting assembly (31) controls the lifting of the second cap screwing assembly (29) to complete the cap screwing operation.

8. The sample pretreatment device according to claim 1, characterized by The magnetic separation and reagent transfer mechanism (7) comprises a fifth clamping assembly (33) for clamping and positioning the centrifugal tube, a fifth linear module (34) for driving the fifth clamping assembly to move, a magnetic bead adding assembly (35) for adding magnetic beads into the centrifugal tube, a magnetic separation assembly (36) for magnetically separating the sample in the centrifugal tube, an adding and reagent transfer assembly (37) for adding reagent into the centrifugal tube and transferring the separated liquid to the carrier module on the second main track, and a centrifugal tube waste box (38) for storing the waste centrifugal tube after magnetic separation; when the carrier module runs to the position of the fifth clamping assembly (33), the blocking cylinder is limited, the fifth clamping assembly (33) clamps the centrifugal tube, the fifth linear module (34) moves the centrifugal tube to the position of adding magnetic beads, and the magnetic bead adding assembly (35) adds magnetic beads into the centrifugal tube; then the adding and reagent transfer assembly (37) adds reagent into the centrifugal tube, and the magnetic separation assembly (36) magnetically separates the sample; after separation is completed, the adding and reagent transfer assembly (37) transfers the separated liquid to the carrier module on the second main track, and the fifth linear module (34) moves the waste centrifugal tube to the centrifugal tube waste box (38) for discarding.

9. The sample pretreatment device according to claim 1, characterized by The centrifugal tube loading mechanism (8) comprises a sixth clamping assembly (39) for clamping the centrifugal tube, a sixth linear module (40) for driving the sixth clamping assembly to move, and a centrifugal tube loading assembly (41); when the carrier module runs to the position of the sixth clamping assembly (39), the blocking cylinder is limited, the sixth clamping assembly (39) clamps the centrifugal tube provided by the centrifugal tube loading assembly (41), and the centrifugal tube is transferred to the carrier module through the sixth linear module (40).

10. The sample pretreatment device according to claim 1, characterized by, The nitrogen blowing mechanism (9) comprises a seventh clamping assembly (42) for clamping the centrifugal tube, a seventh linear module (43) for driving the seventh clamping assembly to move, and a nitrogen blowing instrument (44) for nitrogen blowing treatment of the sample in the centrifugal tube; when the sample module runs to the position of the seventh clamping assembly (42), the seventh clamping assembly (42) clamps the centrifugal tube in the sample module, and the centrifugal tube is transferred to the nitrogen blowing instrument (44) for nitrogen blowing treatment through the seventh linear module (43); after the nitrogen blowing is completed, the seventh linear module (43) transfers the centrifugal tube back to the sample module.

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