Oncolytic virus purification and separation device

Abstract

The utility model relates to the technical field of virus purification and centrifugal separation equipment, and discloses an oncolytic virus purification and separation device which comprises a box seat, a driving motor and a plurality of bins, at the beginning of rotation, the electric telescopic rod is controlled to enable the second support frame to slowly descend, so that the test tube can be gradually close to the level, and the oncolytic virus can be conveniently separated from the bottom of the test tube, and in the process that the driving motor stops providing power to enable the first support frame to rotate at an idle speed, the electric telescopic rod is controlled to enable the second support frame to slowly ascend, so that the test tube can be gradually close to the vertical state; in the resetting process of the test tube, the state of a solution in the test tube does not change greatly, and the solution is layered at the bottom of the test tube, so that the problem that purification and separation products of oncolytic viruses are mixed is effectively avoided, the pressing nut is controlled to rotate according to the length of the test tube, the bin body adapts to test tubes of different sizes, and meanwhile, the bin body is benefited from the supporting pad and the traction spring; and the test tubes are more convenient to take out while the pressure intensity of the test tubes in the bin body is relatively small.

Description

Technical Field

[0001] This utility model relates to the technical field of virus purification and centrifugal separation equipment, specifically an oncolytic virus purification and separation device. Background Technology

[0002] The oncolytic virus purification and separation device is a device that uses the principle of centrifugal force to separate and purify viruses. Its main function is to use the powerful centrifugal force generated by high-speed rotation to rapidly separate, concentrate and purify mixtures of different densities, masses and properties. It is widely used in biomedical research and clinical applications.

[0003] Chinese patent CN217535986U discloses a device for purifying and separating oncolytic viruses, including a centrifuge base, a rotor inside the centrifuge base, a rotating chamber on the outer side wall of the upper part of the rotor, a first annular sieve plate and a second annular sieve plate inside the rotating chamber, the second annular sieve plate being located outside the first annular sieve plate, a receiving cone at the upper end of the rotor, a discharge pipe at the upper end of the receiving cone, a rotatable closing cover on the outside of the discharge pipe, and a liquid outlet unit at the bottom of the rotating chamber located between the first and second annular sieve plates. This invention can quickly purify and concentrate antigen solutions, and it has a small footprint and is easy to replace and maintain.

[0004] The aforementioned device is convenient for replacement and maintenance. However, the tilt angle of the chamber of the existing device is fixed during use, which causes the oncolytic virus solution to fall due to gravity after centrifugation. At the same time, the test tubes need to be uprighted when picking them up and putting them in, which causes the oncolytic virus centrifugation products to mix together when falling and when the test tubes are upright, affecting the purification and separation effect. In addition, the pressure at the bottom of the test tube is relatively high during centrifugation. Therefore, there is a need to provide an oncolytic virus purification and separation device. Utility Model Content

[0005] The purpose of this invention is to provide an oncolytic virus purification and separation device to solve the problems of easy mixing of solutions and high pressure between the chamber and the bottom of the test tube during the centrifugation process of existing devices for purifying and separating oncolytic viruses.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an oncolytic virus purification and separation device, comprising a base, a drive motor, and several chambers, wherein a tilting mechanism is provided on the chambers; the tilting mechanism includes an electric telescopic rod, the electric telescopic rod is fixedly installed at the bottom of the base, and a first support is rotatably installed between the output end of the electric telescopic rod and the base, and a second support is rotatably installed on the output end of the electric telescopic rod, and a cap is threadedly installed on the output end of the electric telescopic rod; a ball bearing assembly is fixedly installed between the second support, the output end of the electric telescopic rod, and the cap, and several sliding seats are slidably installed on the second support.

[0007] Preferably, the output end of the drive motor is connected to the first support frame via gears and a gear ring, and retaining rings are fixedly installed on the output end of the electric telescopic rod, the second support frame, and the buckle.

[0008] Preferably, the lower part of the hopper is rotatably mounted on the first support frame, and the upper part of the hopper is rotatably mounted between two slides, and the tilt angle between the hopper and the first support frame is always less than 80°.

[0009] Preferably, the hopper body is provided with a support mechanism, the support mechanism including a support pad, the support pad being slidably installed inside the hopper body, and a threaded shaft being fixedly installed at the lower part of the support pad and through the hopper body, a pressure nut being threaded on the threaded shaft, and a washer being slidably installed in the middle of the threaded shaft, and a traction spring being fixedly installed between the washer and the hopper body.

[0010] Preferably, the upper part of the support pad is provided with an arc-shaped groove, and both sides of the threaded shaft are provided with strip-shaped grooves, and the pad is adapted to the strip-shaped grooves.

[0011] Preferably, the two ends of the traction spring are fixedly connected to the housing and the gasket, respectively.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] 1) This oncolytic virus purification and separation device controls the insertion of the test tube into the chamber, and further controls the drive motor to rotate the first support. At the beginning of the rotation, the electric telescopic rod is controlled to slowly lower the second support, so that the test tube gradually approaches the horizontal, which facilitates the separation of oncolytic virus at the bottom of the test tube. When the drive motor stops providing power and the first support is idling, the electric telescopic rod is controlled to slowly raise the second support, so that the test tube gradually approaches the vertical. During the repositioning process of the test tube, the state of the solution inside the test tube will not change significantly, and the layers are separated at the bottom of the test tube, effectively avoiding the problem of mixing of oncolytic virus purification and separation products.

[0014] 2) This device for purifying and separating oncolytic viruses changes the position of the gasket on the threaded shaft by controlling the pressure nut, thereby changing the traction force of the traction spring on the threaded shaft, which changes the initial height of the support pad in the chamber. During use, the pressure nut is rotated according to the length of the test tube, so that the chamber can adapt to test tubes of different sizes. At the same time, thanks to the support pad and traction spring, the pressure on the test tube in the chamber is kept low, while making the test tube easier to remove. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural schematic diagram of an oncolytic virus purification and separation device according to an embodiment of the present invention;

[0016] Figure 2 This is a cross-sectional view of the base in an embodiment of the present utility model;

[0017] Figure 3 This is a three-dimensional structural diagram of the ball assembly in an embodiment of the present utility model;

[0018] Figure 4 This is a cross-sectional view of the hopper body in an embodiment of the present invention.

[0019] In the diagram: 1. Box base; 2. Drive motor; 3. Box body; 4. Tilting mechanism; 401. Electric telescopic rod; 402. Support frame 1; 403. Support frame 2; 404. Buckle; 405. Ball bearing assembly; 406. Slide; 5. Support mechanism; 501. Support pad; 502. Threaded shaft; 503. Pressure nut; 504. Washer; 505. Traction spring. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Example 1

[0022] Combination Figures 1-3An oncolytic virus purification and separation device includes a base 1, a drive motor 2, and several chambers 3. A tilting mechanism 4 is provided on each of the chambers 3. The tilting mechanism 4 includes an electric telescopic rod 401, which is fixedly installed at the bottom of the base 1. A first support 402 is rotatably mounted between the output end of the electric telescopic rod 401 and the base 1. A second support 403 is rotatably mounted on the output end of the electric telescopic rod 401, and a cap 404 is threaded onto the output end of the electric telescopic rod 401. Ball bearing assemblies 405 are fixedly installed between the second support 403, the output end of the electric telescopic rod 401, and the cap 404. Several sliding seats 406 are slidably mounted on the second support 403.

[0023] The output end of the drive motor 2 is connected to the first support frame 402 via gear and gear ring meshing. The first support frame 402 is rotatably mounted between the electric telescopic rod 401 and the housing base 1, allowing the drive motor 2 to control the rotation of the first support frame 402. Retaining rings are fixedly installed on the output end of the electric telescopic rod 401, the second support frame 403, and the buckle 404. The retaining rings serve as limiters to prevent the ball bearing assembly 405 from disengaging. The ball bearing assembly 405 reduces the friction between the second support frame 403, the electric telescopic rod 401, and the buckle 404. The lower part of the housing 3 is rotatably mounted on a... The chamber 3 is mounted on the first support 402, and the upper part of the chamber 3 is rotatably installed between two slides 406. The tilt angle between the chamber 3 and the first support 402 is always less than 80°. When the electric telescopic rod 401 is controlled to lower the second support 403, the slide 406 will move on the second support 403 and control the chamber 3 to rotate around the first support 402. When the first support 402 is about to stop rotating, the electric telescopic rod 401 is slowly controlled to raise the second support 403, so that the chamber 3 gradually approaches a vertical state, and the oncolytic virus separation solution is relatively smoothly separated into layers in the test tube.

[0024] Example 2

[0025] See Figure 4 A support mechanism 5 is provided on the hopper body 3. The support mechanism 5 includes a support pad 501, which is slidably installed inside the hopper body 3. A threaded shaft 502 is fixedly installed on the lower part of the support pad 501 and through the hopper body 3. A pressure nut 503 is threaded on the threaded shaft 502, and a washer 504 is slidably installed in the middle of the threaded shaft 502. A traction spring 505 is fixedly installed between the washer 504 and the hopper body 3.

[0026] The upper part of the support pad 501 has an arc-shaped groove so that the bottom of the test tube can be wrapped in the arc-shaped groove. Both sides of the threaded shaft 502 have strip-shaped grooves, and the gasket 504 is adapted to the strip-shaped grooves. When the pressure nut 503 is rotated, the gasket 504 will move up and down under the support of the pressure nut 503, thereby changing the traction force of the gasket 504 on the traction spring 505, thus changing the initial height and buffer force of the support pad 501 in the chamber 3. The two ends of the traction spring 505 are fixedly connected to the chamber 3 and the gasket 504 respectively.

[0027] In actual operation, the drive motor 2 can control the rotation of the first support 402. The output end of the electric telescopic rod 401, the second support 403, and the buckle 404 are all fixedly installed with retaining rings. The retaining rings play a limiting role to prevent the ball assembly 405 from disengaging. The ball assembly 405 can reduce the friction between the second support 403, the electric telescopic rod 401, and the buckle 404. When the electric telescopic rod 401 is controlled to lower the second support 403, the slide 406 will move on the second support 403 and control the chamber 3 to rotate around the first support 402. When the first support 402 is about to stop rotating, the electric telescopic rod 401 is slowly controlled to raise the second support 403, so that the chamber 3 gradually approaches the vertical state, and the oncolytic virus separation solution is separated into layers in the test tube more smoothly.

[0028] The upper part of the support pad 501 has an arc-shaped groove so that the bottom of the test tube can be wrapped in the arc-shaped groove. Both sides of the threaded shaft 502 have strip-shaped grooves, and the gasket 504 is adapted to the strip-shaped grooves. When the pressure nut 503 is rotated, the gasket 504 will move up and down under the support of the pressure nut 503, thereby changing the traction force of the gasket 504 on the traction spring 505, thus changing the initial height and buffer force of the support pad 501 in the chamber 3. The two ends of the traction spring 505 are fixedly connected to the chamber 3 and the gasket 504 respectively.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An oncolytic virus purification and separation device, comprising a base (1), a drive motor (2), and several chambers (3), characterized in that: A tilting mechanism (4) is provided on several of the aforementioned compartments (3); The tilting mechanism (4) includes an electric telescopic rod (401), which is fixedly installed at the bottom of the base (1). A first support (402) is rotatably installed between the output end of the electric telescopic rod (401) and the base (1). A second support (403) is rotatably installed on the output end of the electric telescopic rod (401). A buckle (404) is threaded onto the output end of the electric telescopic rod (401). A ball bearing assembly (405) is fixedly installed between the second support (403), the output end of the electric telescopic rod (401), and the buckle (404). Several slide blocks (406) are slidably installed on the second support (403).

2. The oncolytic virus purification and separation device according to claim 1, characterized in that: The output end of the drive motor (2) is connected to the first support frame (402) through gear and gear ring meshing. The output end of the electric telescopic rod (401), the second support frame (403) and the buckle (404) are all fixedly installed with retaining rings.

3. The oncolytic virus purification and separation device according to claim 1, characterized in that: The lower part of the silo body (3) is rotatably mounted on the first support frame (402), and the upper part of the silo body (3) is rotatably mounted between two slides (406). The tilt angle between the silo body (3) and the first support frame (402) is always less than 80°.

4. The oncolytic virus purification and separation device according to claim 3, characterized in that: A support mechanism (5) is provided on the hopper (3). The support mechanism (5) includes a support pad (501). The support pad (501) is slidably installed inside the hopper (3). A threaded shaft (502) is fixedly installed on the lower part of the support pad (501) and through the hopper (3). A pressure nut (503) is threaded on the threaded shaft (502). A washer (504) is slidably installed in the middle of the threaded shaft (502). A traction spring (505) is fixedly installed between the washer (504) and the hopper (3).

5. The oncolytic virus purification and separation device according to claim 4, characterized in that: The upper part of the support pad (501) is provided with an arc-shaped groove, and both sides of the threaded shaft (502) are provided with strip-shaped grooves, and the gasket (504) is adapted to the strip-shaped grooves.

6. The oncolytic virus purification and separation device according to claim 5, characterized in that: The two ends of the traction spring (505) are fixedly connected to the housing (3) and the gasket (504) respectively.

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