Extraction device of magnetic bead purification instrument

Abstract

The utility model discloses an extraction device of a magnetic bead purification instrument, the extraction device comprises a magnetic extraction mechanism and a moving mechanism, the magnetic extraction mechanism comprises a magnetic bar assembly and a magnetic bar sleeve assembly; the magnetic bar assembly comprises a magnetic bar and a fixing frame used for fixing the magnetic bar, the fixing frame is connected with the moving mechanism, and the magnetic bar can move horizontally and vertically along with the fixing frame under the driving of the moving mechanism; the magnetic bar sleeve assembly comprises magnetic bar sleeves in one-to-one correspondence with the magnetic bars and a fixing plate used for fixing the magnetic bar sleeves, the fixing plate is located under the magnetic bars, through holes matched with the magnetic bar sleeves are formed in the fixing plate, the upper ends of the magnetic bar sleeves are fixed to the through holes, and the lower ends of the magnetic bar sleeves are fixed to the fixing plate. And the magnetic bar sleeve can be driven by the moving mechanism to do horizontal movement and vertical movement. By improving an existing extraction device, the extraction amount of substances is increased, the detection sensitivity is improved, and the extraction process is simplified.

Description

Technical Field

[0001] This utility model relates to a magnetic bead purification device, specifically to an extraction device for a magnetic bead purification instrument. Background Technology

[0002] Magnetic bead purification systems utilize the magnetic properties of magnetic microparticles to adsorb target molecules (such as proteins, DNA, or RNA) onto the surface of magnetic beads. Then, magnetic force is used to separate the beads and the adsorbed molecules, thus achieving the separation and purification of biological samples. Magnetic bead purification systems are primarily used for the rapid purification of nucleic acids, proteins, and cells. Employing magnetic bead separation and extraction purification technology, they can automatically perform steps such as mixing, bead transfer, washing, and elution. Magnetic bead purification systems have wide applications in biomedical research, clinical diagnostics, and drug development. They can rapidly and efficiently separate and purify target molecules from biological samples, providing high-quality samples for subsequent experiments and analyses, playing a crucial role in biomedical research and clinical diagnostics.

[0003] The extraction mechanism of a magnetic bead purifier is the core component in the magnetic bead purification process. It is responsible for the adsorption, transfer, and release of magnetic beads, thereby completing the extraction of nucleic acids or proteins. During the extraction process, magnetic beads are added to a sample containing nucleic acids or proteins. Through specific chemical reactions or physical actions, they bind to the target molecules. Under the influence of a magnetic field, the magnetic beads, along with their bound target molecules, are adsorbed onto the magnetic rods or poles of the extraction mechanism. Then, by moving the magnetic rods or poles, the magnetic beads and their bound target molecules are transferred to different reagent tanks or containers to achieve the extraction of substances such as nucleic acids or proteins.

[0004] When extracting trace amounts of nucleic acids or proteins, conventional magnetic bead purification methods may not be able to effectively extract sufficient quantities, leading to decreased detection sensitivity and affecting both efficiency and purity. Furthermore, the magnetic bead purification process requires the use of various reagents and equipment, increasing the complexity and cost of the operation. Utility Model Content

[0005] In order to overcome the shortcomings of the prior art, the purpose of this application is to provide an extraction device for a magnetic bead purification instrument, which improves the extraction yield, enhances detection sensitivity, and simplifies the extraction process by improving the existing extraction device.

[0006] To solve the above problems, the technical solution adopted in this application is as follows:

[0007] This application provides an extraction device for a magnetic bead purification instrument, including a magnetic extraction mechanism and a moving mechanism. The magnetic extraction mechanism includes a magnetic rod assembly and a magnetic rod sleeve assembly. The magnetic rod assembly includes a magnetic rod and a fixing frame for fixing the magnetic rod. The fixing frame is connected to the moving mechanism, and the magnetic rod can move horizontally and vertically under the drive of the moving mechanism. The magnetic rod sleeve assembly includes magnetic rod sleeves corresponding to the magnetic rods and a fixing plate for fixing the magnetic rod sleeves. The fixing plate is located directly below the magnetic rods, and the fixing plate is provided with through holes that match the magnetic rod sleeves. The upper end of the magnetic rod sleeve is fixed at the through hole, and the magnetic rod sleeve can move horizontally and vertically under the drive of the moving mechanism.

[0008] As a further preferred embodiment, the magnetic rod moving mechanism described in this application includes a horizontal driving mechanism and a vertical driving mechanism. The horizontal driving mechanism includes a horizontal driving motor that causes the magnetic extraction mechanism to move horizontally and a horizontal guide rail. The fixing frame includes a sliding seat mounted on the horizontal guide rail and a mounting bracket for mounting the magnetic rod. The sliding seat is connected to the horizontal driving motor, and the horizontal driving motor drives the sliding seat to reciprocate between a starting position and an ending position along the horizontal guide rail. The vertical driving mechanism includes a first driving motor for driving the magnetic rod to move vertically and a second driving motor for driving the magnetic rod sleeve to move vertically. The sliding seat is provided with a vertical guide rail for mounting the mounting bracket and the fixing plate. The first driving motor drives the mounting bracket to move the magnetic rod vertically along the vertical guide rail, and the second driving motor drives the fixing plate to move the magnetic rod sleeve vertically along the vertical guide rail.

[0009] As a further preferred embodiment, the horizontal guide rail described in this application is a linear guide rail, and the magnetic extraction mechanism can reciprocate along the linear guide rail.

[0010] As a further preferred embodiment, the horizontal guide rail described in this application is a ring-shaped guide rail, and the magnetic extraction mechanism can perform cyclical movement along the ring-shaped guide rail.

[0011] As a further preferred embodiment, the number of magnetic rods described in this application is 6, 12, or 24.

[0012] As a further preferred embodiment, the extraction device of the magnetic bead purifier described in this application further includes a liquid-blocking assembly; the liquid-blocking assembly includes a liquid-blocking plate and a liquid-blocking plate driving mechanism for driving the liquid-blocking plate to move horizontally.

[0013] As a further preferred embodiment, the liquid shielding plate driving mechanism described in this application includes a drive motor, a gear, and a rack meshing with the gear. The gear is connected to the drive motor, and the drive motor drives the gear to reciprocate along the rack.

[0014] As a further preferred embodiment, the mounting bracket described in this application includes a mounting plate and a mounting frame, wherein the magnetic rod is fixed on the mounting plate, and the mounting plate is fixed on the mounting frame by a snap-fit ​​or screw.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] 1. The extraction device of the magnetic bead purification instrument described in this application combines a magnetic extraction mechanism with a moving mechanism. Both the magnetic rod assembly and the magnetic rod sleeve assembly can move horizontally and vertically under the drive of the moving mechanism, which makes the steps of magnetic bead adsorption, separation and washing more flexible and improves the convenience and efficiency of operation.

[0017] 2. The magnetic rod assembly and magnetic rod sleeve assembly of the extraction device of the magnetic bead purification instrument described in this application move in coordination, which helps to achieve precise control of the magnetic beads during the magnetic bead purification process. This can not only improve the recovery rate of magnetic beads, but also reduce the loss and contamination of magnetic beads, thereby improving the quality and purity of purification.

[0018] 3. Furthermore, the extraction device of the magnetic bead purification instrument described in this application is equipped with a fixing frame and a fixing plate, so that the magnetic rod and magnetic rod sleeve can be stably fixed in the predetermined position, avoiding problems such as shaking or falling off during operation; at the same time, this design also facilitates the cleaning and maintenance of the device, extending the service life of the equipment.

[0019] 4. Furthermore, in the extraction device of the magnetic bead purification instrument described in this application, since both the magnetic rod assembly and the magnetic rod sleeve assembly can move horizontally and vertically, the extraction device can adapt to sample containers of different sizes and shapes, thus improving the versatility and adaptability of the equipment.

[0020] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the extraction device structure of the magnetic bead purification instrument described in the embodiments of this application.

[0023] Figure 2 This is a partial structural diagram of the magnetic rod assembly and magnetic rod sleeve assembly described in the embodiments of this application.

[0024] The reference numerals in the attached figures are as follows: 10, magnetic rod assembly; 11, magnetic rod; 12, fixing frame; 121, sliding seat; 122, mounting bracket; 20, magnetic rod sleeve assembly; 21, magnetic rod sleeve; 22, fixing plate; 23, through hole; 30, horizontal drive mechanism; 31, horizontal drive motor; 32, horizontal guide rail; 40, vertical drive mechanism; 41, first drive motor; 42, second drive motor; 50, liquid shielding plate; 60, liquid shielding plate drive mechanism; 61, drive motor; 62, gear; 63, rack. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0026] The term "comprising" and other equivalent descriptive terms used in the specification and claims of this application are intended to cover a non-exclusive inclusion, which includes both the contents explicitly described in the specification and claims and steps or units that are not described in the specification and claims but are inherent in the product, method or structure.

[0027] like Figure 1 , Figure 2As shown, this application embodiment provides an extraction device for a magnetic bead purification instrument, which is fixed to the base of the magnetic bead purification instrument by a crossbeam and a horizontal beam; it includes a magnetic extraction mechanism and a moving mechanism. The magnetic extraction mechanism includes a magnetic rod assembly 10 and a magnetic rod sleeve assembly 20. The magnetic extraction mechanism is the core part of the extraction device, used to capture, move, and release magnetic beads through magnetic action, thereby achieving the purification of target substances. The magnetic rod assembly 10 includes a magnetic rod 11 and a fixing frame 12 for fixing the magnetic rod. The magnetic rod 11 can attract and fix magnetic beads, which are usually used to bind and separate substances such as proteins, nucleic acids, and plasmids in biological samples; the fixing frame 12 can firmly fix the magnetic rod 11 to prevent it from moving or falling off during operation. The fixed frame 12 is connected to the moving mechanism. The magnetic rod 11 can move horizontally and vertically under the drive of the moving mechanism along with the fixed frame 12. Through the movement of the magnetic rod, precise control of the magnetic beads and the target substances they are bonded to can be achieved, such as mixing, separating, and extracting. The moving mechanism provides power to the magnetic extraction mechanism, enabling it to move along a predetermined trajectory and speed. By precisely controlling the movement of the moving mechanism, precise control of the magnetic beads and target substances can be achieved, improving the accuracy and efficiency of the experiment. The magnetic rod sleeve assembly 20 includes magnetic rod sleeves 21 corresponding to magnetic rods 11 and fixing plates 22 for fixing the magnetic rod sleeves 21. The magnetic rod sleeves 21 are used to indirectly manipulate magnetic beads through magnetic action when the magnetic rods do not directly contact the sample solution, which helps to reduce the contamination and interference of the magnetic rods on the sample solution. The fixing plate provides stable support and fixation, ensuring the stability and accuracy of the magnetic rod sleeves during movement. The fixing plate 22 is located directly below the magnetic rods 11. The fixing plate 22 is provided with through holes 23 that match the magnetic rod sleeves 21. The upper end of the magnetic rod sleeves 21 is fixed at the through holes 23. The magnetic rod sleeves can move horizontally and vertically under the drive of the moving mechanism. In the scheme of this application, the extraction device of the magnetic bead purifier achieves precise control and purification of magnetic beads and target substances through the synergistic effect of various components; among them, the magnetic extraction mechanism is responsible for capturing and moving the magnetic beads, the moving mechanism provides power support, and the magnetic rod sleeve assembly reduces contamination and interference to the sample solution by indirectly controlling the magnetic beads; the cooperation between these components constitutes a highly efficient and accurate magnetic bead purification system.

[0028] In some specific embodiments of this application, the magnetic rod moving mechanism includes a horizontal drive mechanism 30 and a vertical drive mechanism 40. The horizontal drive mechanism 30 includes a horizontal drive motor 31 and a horizontal guide rail 32 that cause the magnetic extraction mechanism to move horizontally. The fixing frame 12 includes a sliding seat 121 mounted on the horizontal guide rail 32 and a mounting bracket 122 for mounting the magnetic rod 21. The sliding seat 121 is connected to the horizontal drive motor 31, which drives the sliding seat 121 to reciprocate between the starting position and the ending position along the horizontal guide rail. Driven by the horizontal drive motor, the sliding seat reciprocates on the horizontal guide rail, allowing the magnetic extraction mechanism to move flexibly between the starting position and the ending position, meeting the needs of different positions during the experiment. The horizontal guide rail ensures the smooth operation of the sliding seat, reduces friction and vibration during movement, and improves the accuracy and stability of the movement. The vertical drive mechanism 40 includes a first drive motor 41 for driving the magnetic rod to move vertically and a second drive motor 42 for driving the magnetic rod sleeve to move vertically. The sliding seat 121 is equipped with a vertical guide rail (not shown in the figure) for mounting the mounting bracket 122 and the fixing plate 22. The first drive motor 41 drives the mounting bracket 122, causing the magnetic rod 11 to move vertically along the vertical guide rail. The second drive motor 42 drives the fixing plate 22, causing the magnetic rod sleeve 21 to move vertically along the vertical guide rail. The cooperation of the first drive motor and the transmission mechanism allows the magnetic rod to move up and down in the vertical direction, meeting the requirements of steps such as the binding and separation of the magnetic bead with the target substance during the experiment. The second drive motor causes the magnetic rod sleeve to move up and down in the vertical direction. This design allows the magnetic rod sleeve to flexibly cooperate with the movement of the magnetic rod to complete the capture and release of the magnetic bead. The straightness and precision of the vertical guide rail ensure the smooth operation of the mounting bracket and the fixing plate, reducing friction and vibration during movement and improving the accuracy and stability of the movement.

[0029] Based on the above scheme, in Embodiment 1, the horizontal guide rail is a linear guide rail, and the magnetic extraction mechanism can reciprocate along the linear guide rail. The linear guide rail ensures that the magnetic extraction mechanism maintains extremely high stability and accuracy during horizontal movement; moreover, the linear guide rail typically uses rolling or sliding friction pairs, which have a lower coefficient of friction compared to other types of guide rails, reducing energy loss and wear during movement; the smooth movement and high precision of the linear guide rail ensure that the magnetic extraction mechanism can move quickly and accurately to the designated position, helping to shorten experimental time, improve experimental efficiency, and reduce the experimental failure rate caused by inaccurate movement.

[0030] Based on the above scheme, in Embodiment 1, the horizontal guide rail is a ring-shaped guide rail, and the magnetic extraction mechanism can circulate along the ring-shaped guide rail. In this scheme, on the one hand, the closed-loop structure of the ring-shaped guide rail allows the magnetic extraction mechanism to continuously circulate without returning to the starting point after each movement, effectively improving the continuity and efficiency of the experiment; it is suitable for experimental scenarios requiring long-term, continuous processing, such as the purification of large-scale samples using magnetic beads. On the other hand, using a ring-shaped guide rail eliminates the need for additional space to accommodate the return path, making it more compact and space-saving. The ring-shaped guide rail can also be customized according to experimental needs, including the diameter, shape, and speed of the track, to adapt to different experimental conditions and sample processing requirements, enabling the magnetic extraction mechanism to flexibly adapt to various experimental scenarios and effectively improve the equipment's versatility and adaptability. Furthermore, the design of the ring-shaped guide rail makes it easier to integrate the magnetic extraction mechanism with an automated control system, achieving automation and intelligence in the experiment; the connection points between the curved and straight sections of the ring-shaped guide rail are usually smoothly transitioned, helping to reduce vibration and noise during high-speed operation.

[0031] Based on the above embodiment 1 or embodiment 2, as a further preferred embodiment, the number of magnetic rods in this application is 6, 12, 24, 48 or 96, etc., corresponding to 6-well plates, 12-well plates, 24-well plates, 48-well plates, etc., and different ones can be provided as needed.

[0032] Based on the solutions of Embodiment 1 or Embodiment 2 described above, the extraction device of the magnetic bead purifier described in this application further includes a liquid-shielding assembly; the liquid-shielding assembly includes a liquid-shielding plate 50 and a liquid-shielding plate driving mechanism 60 for driving the liquid-shielding plate 50 to move horizontally. On the one hand, the main function of the liquid-shielding plate is to shield and cover the area where liquid may splash during the magnetic bead purification process; through horizontal movement, the liquid-shielding plate can precisely cover or move away to control the exposure and splashing of liquid, thereby preventing liquid from splashing out and contaminating the experimental environment or equipment. On the other hand, the liquid-shielding plate can also prevent external impurities or air from entering the sample solution, protecting the purity and integrity of the sample. The liquid-shielding plate can also reduce the loss of magnetic beads during the purification process, improving the utilization rate of magnetic beads and purification efficiency. The liquid-shielding assembly plays a crucial role in the extraction device of the magnetic bead purifier; it not only improves the safety and accuracy of the experiment, but also optimizes the experimental process and enhances the functionality of the equipment. The liquid-shielding plate is made of corrosion-resistant and easy-to-clean materials, such as stainless steel, plastic, or special alloys, to ensure that its performance and appearance can be maintained during long-term use. The liquid shielding plate drive mechanism can use a stepper motor, servo motor or DC motor to ensure that the liquid shielding plate can move accurately and smoothly.

[0033] As a further preferred embodiment, the liquid-blocking plate driving mechanism described in this application includes a drive motor 61, a gear 62, and a rack 63 meshing with the gear 62. The gear is connected to the drive motor, and the drive motor drives the gear to reciprocate along the rack. In this embodiment, the gear and rack serve as a transmission mechanism to convert the rotational motion of the motor into the horizontal motion of the liquid-blocking plate. Besides the combination of gears and racks, structures such as lead screws, guide rails, and sliders can also be used as transmission mechanisms.

[0034] In the embodiments of this application, the drive motors involved, such as horizontal drive motors, first drive motors, and second drive motors, can be selected from, but are not limited to, stepper motors, servo motors, or DC motors.

[0035] As a further preferred embodiment, the mounting bracket described in this application includes a mounting plate and a mounting frame, wherein the magnetic rod is fixed on the mounting plate, and the mounting plate is fixed on the mounting frame by a snap-fit ​​or screw.

[0036] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. An extraction device for a magnetic bead purification instrument, characterized in that, The device includes a magnetic extraction mechanism and a moving mechanism. The magnetic extraction mechanism includes a magnetic rod assembly and a magnetic rod sleeve assembly. The magnetic rod assembly includes a magnetic rod and a fixing frame for fixing the magnetic rod. The fixing frame is connected to the moving mechanism, and the magnetic rod can move horizontally and vertically under the drive of the moving mechanism. The magnetic rod sleeve assembly includes a magnetic rod sleeve corresponding to each magnetic rod and a fixing plate for fixing the magnetic rod sleeve. The fixing plate is located directly below the magnetic rod and has a through hole that matches the magnetic rod sleeve. The upper end of the magnetic rod sleeve is fixed at the through hole, and the magnetic rod sleeve can move horizontally and vertically under the drive of the moving mechanism.

2. The extraction device of the magnetic bead purification instrument according to claim 1, characterized in that, The magnetic rod moving mechanism includes a horizontal drive mechanism and a vertical drive mechanism. The horizontal drive mechanism includes a horizontal drive motor that enables the magnetic extraction mechanism to move horizontally and a horizontal guide rail. The fixing frame includes a sliding seat mounted on the horizontal guide rail and a mounting bracket for mounting the magnetic rod. The sliding seat is connected to the horizontal drive motor, and the horizontal drive motor drives the sliding seat to reciprocate between a starting position and an ending position along the horizontal guide rail. The vertical drive mechanism includes a first drive motor for driving the magnetic rod to move vertically and a second drive motor for driving the magnetic rod sleeve to move vertically. The sliding seat is provided with a vertical guide rail for mounting the mounting bracket and the fixing plate. The first drive motor drives the mounting bracket to move the magnetic rod vertically along the vertical guide rail, and the second drive motor drives the fixing plate to move the magnetic rod sleeve vertically along the vertical guide rail.

3. The extraction device of the magnetic bead purification instrument according to claim 2, characterized in that, The horizontal guide rail is a linear guide rail, and the magnetic extraction mechanism can reciprocate along the linear guide rail.

4. The extraction device of the magnetic bead purification instrument according to claim 2, characterized in that, The horizontal guide rail is a ring-shaped guide rail, and the magnetic extraction mechanism can move cyclically along the ring-shaped guide rail.

5. The extraction device of the magnetic bead purification instrument according to claim 1, characterized in that, The number of magnetic rods is 6, 12, or 24.

6. The extraction device of the magnetic bead purification instrument according to claim 1, characterized in that, It also includes a liquid-blocking assembly; the liquid-blocking assembly includes a liquid-blocking plate and a liquid-blocking plate driving mechanism for driving the liquid-blocking plate to move horizontally.

7. The extraction device of the magnetic bead purification instrument according to claim 6, characterized in that, The liquid shielding plate driving mechanism includes a drive motor, a gear, and a rack meshing with the gear, wherein the gear is connected to the drive motor.

8. The extraction device of the magnetic bead purification instrument according to any one of claims 2-4, characterized in that, The mounting bracket includes a mounting plate and a mounting frame. The magnetic rod is fixed on the mounting plate, and the mounting plate is fixed on the mounting frame by a snap-fit ​​or screw.

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