Microfluidic separation device for separating puccinia striiformis spores
By designing a microfluidic separation device, large particulate impurities are filtered out using a primary filter tube. The pretreatment zone and orthogonally coupled separation zone in the microfluidic chip achieve efficient separation of wheat stripe rust urediniospores, solving the detection problem in existing technologies and achieving efficient and low-cost spore separation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-29
- Publication Date
- 2026-04-14
AI Technical Summary
Existing spore collection technologies are difficult to achieve rapid and accurate detection of wheat stripe rust urediniospores with high precision and high throughput, and are also costly and complex to operate.
A microfluidic separation device is designed using microfluidic technology, including a primary selection mechanism, a separation chamber, and a microfluidic chip. Large particulate impurities are filtered through the primary selection chamber, and the pretreatment zone, axial focusing zone, and orthogonal coupling separation zone in the microfluidic chip achieve efficient separation of spores.
It achieves efficient collection of wheat stripe rust urediniospores, with simple operation, high separation efficiency, and large throughput, and can accurately separate virus spores of different particle sizes.
Smart Images
Figure CN224118997U_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of viral spore isolation technology, specifically a microfluidic separation device for separating stripe rust spores. Background Technology
[0002] Wheat stripe rust is a disease spread through airborne urediniospores. The key to controlling wheat stripe rust lies in monitoring and early warning of the disease in key affected fields, as urediniospore concentration is highly correlated with the disease status in a field. Therefore, large-scale, rapid, and accurate detection of urediniospores is crucial for monitoring and early warning of wheat stripe rust and preventing its spread. Urediniospores are approximately 25-35 micrometers in size and weigh only a few picograms. Existing spore collection technologies typically employ traditional volumetric spore collectors combined with image observation or biological methods. However, these methods result in low collection concentrations, high costs, and complex operations, making it difficult to achieve high-precision, high-throughput collection of wheat stripe rust urediniospores.
[0003] In recent years, with the rapid development of microfluidic technology, microfluidic chips have become a hot topic in disciplines such as biology, chemistry, and materials science due to their advantages such as low sample consumption, high throughput, high sensitivity, and environmental friendliness. A typical microfluidic chip detection system includes functional units such as sample preparation, transport, pretreatment, injection, mixing, reaction, separation, enrichment, detection, and analysis. Compared with traditional methods, the biggest advantage of microfluidics lies in creating a controllable microenvironment, which can precisely drive and control the microfluidic flow within the microchannel, thereby improving detection sensitivity and having an inherent advantage in the rapid detection of microorganisms. Moreover, impactors designed based on aerodynamic principles are widely used in the separation of atmospheric particles with good results. It is theoretically entirely feasible to collect fungal spores using microfluidic chips with an impactor structure, and this method has high collection efficiency and low cost. Therefore, this utility model proposes a microfluidic separation device for separating stripe rust spores to solve the above problems. Utility Model Content
[0004] The purpose of this invention is to provide a microfluidic separation device for separating stripe rust spores, which achieves efficient separation of spores of different sizes through microfluidic technology, solving the problems of high difficulty, high cost and complex operation in the existing technology of spore separation.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a microfluidic separation device for separating stripe rust spores, comprising...
[0006] The preliminary selection mechanism includes an air inlet end, an umbrella-shaped air collection cap is provided at the upper end of the air inlet end, and a number of air inlets are evenly distributed in a ring array on the air inlet end. The lower end of the air inlet end is fixedly connected to the preliminary selection cylinder by a thread, and the lower end of the preliminary selection cylinder is fixedly connected to the settling hopper by a thread. The lower end of the settling hopper is inserted into the separation box.
[0007] The separation chamber has an insertion slot on one side of its upper end. The initial selection mechanism is inserted into the upper end of the insertion slot. An enrichment chamber is formed inside the separation chamber at the lower end of the insertion slot. One end of the enrichment chamber is connected to an airflow inlet, which is connected to the outside of one side of the separation chamber. The other end of the enrichment chamber is connected to an air supply pipe, the end of which is connected to a microfluidic chip. The other end of the microfluidic chip is connected to the outside of the other side of the separation chamber.
[0008] Preferably, an exhaust fan is provided at the lower end of the air inlet, the exhaust fan is connected to a power source and a switch via a wire core, and a threaded connector is provided at the lower end of the air inlet, the air inlet being fixedly connected to the upper end of the primary selection cylinder via the threaded connector.
[0009] Preferably, a filter structure is provided inside the lower end of the primary selection cylinder. The filter structure is a filter screen, which is fixed to the inner wall of the lower end of the primary selection cylinder by a fixing member. A threaded joint is provided at the lower end of the primary selection cylinder, and the primary selection cylinder is fixed to the upper end of the settling hopper by the threaded structure.
[0010] Preferably, the upper end of the separation box is provided with an insertion slot corresponding to the settling hopper, the lower end of the insertion slot is connected to a disc-shaped enrichment cavity, one side of the enrichment cavity is connected to an airflow inlet, and the outside of the airflow inlet is connected to an air jet device.
[0011] Preferably, the thickness of the microfluidic chip is 3 mm. The microfluidic chip includes a spore airflow inlet on one side, the range of spore airflow is 5 mm / s to 30 mm / s, the basic value is 5 mm / s, the spore airflow inlet is connected to a microfluidic channel, the height of the microfluidic channel is 70 μm, the end of the microfluidic channel is connected to a pretreatment area, the pretreatment area is set as a curved channel to form a filter impact plate structure, and the end of the pretreatment area is connected to an axial focusing area.
[0012] Preferably, the axial focusing area has a Y-shaped structure, including a microfluidic channel in the middle that is connected to the curved channel of the pretreatment area, and sheath airflow channels extending on both sides of the microfluidic channel. An axial sheath inflow hole is opened on the front of the end of the sheath airflow channel. The end of the microfluidic channel is connected to the positive coupling separation area. The flow velocity of the axial sheath flow is 10 mm / s, which is twice the airflow inlet velocity.
[0013] Preferably, the square impact area of the positive coupling separation zone is connected to the sheath airflow channel at the lower end of the impact area. A radial sheath inflow hole is opened at the end of the sheath airflow channel. An air outlet is connected to the end of the impact area. The air outlet is connected to the exhaust hole opened on the side of the separation box. The flow velocity of the radial sheath flow is 20 mm / s, which is 4 times the airflow inlet velocity.
[0014] Compared with the prior art, the beneficial effects of this utility model are:
[0015] This invention achieves efficient collection of wheat stripe rust urediniospores using microfluidic technology. It boasts advantages such as simple operation, high separation efficiency, and large throughput. The initial selection tube allows for air intake and preliminary filtration to remove large particles. The microfluidic chip in the separation chamber agitates the airborne spores into the pretreatment and separation zones for impact separation. The pretreatment zone initially screens particles entering the fluid channel; larger particles collide with and adhere to the impact plate, while smaller particles follow the airflow through the impactor, removing larger air aerosols and aggregated spores. The axial focusing zone uses the compression of the spore-containing airflow in the middle by the double-sheath airflow to focus the spores towards the centerline of the microchannel, ensuring spore movement along the centerline. The orthogonal coupling separation zone, after the spores have been focused, orthogonally couples with the radial sheath flow. Spores of different sizes are separated due to different forces and trajectories, thus accurately collecting wheat stripe rust urediniospores. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the structure of this utility model;
[0017] Figure 2 This is an exploded view of the structure of this utility model;
[0018] Figure 3 This is a perspective view of the separation box structure in this utility model;
[0019] Figure 4 This is a perspective view of the microfluidic chip in this utility model;
[0020] Figure 5 This is a COMSOL simulation diagram of the particle separation microfluidic chip of this utility model.
[0021] In the figure: 1. Inlet end, 2. Inlet hole, 3. Pre-selection tube, 4. Settling hopper, 5. Separation box, 6. Exhaust hole, 7. Insertion slot, 8. Enrichment chamber, 9. Air flow inlet, 10. Gas delivery pipe, 11. Microfluidic chip, 12. Spore air flow inlet, 13. Pretreatment zone, 14. Axial focusing zone, 15. Orthogonal coupling separation zone, 16. Air outlet. Detailed Implementation
[0022] To make the objectives, technical solutions, and advantages of this utility model clear and complete, the embodiments of this utility model will be further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only some, not all, embodiments of this utility model, and are merely used to explain the embodiments of this utility model. They are not intended to limit the embodiments of this utility model. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.
[0023] Please see Figures 1 to 5 This utility model provides a technical solution: a microfluidic separation device for separating stripe rust spores, comprising...
[0024] The primary selection mechanism includes an air inlet 1, an umbrella-shaped air collection cap on the upper end of the air inlet 1, and several air inlets 2 evenly distributed in a ring array on the air inlet 1. The lower end of the air inlet 1 is fixedly connected to the primary selection cylinder 3 by a thread, and the lower end of the primary selection cylinder 3 is fixedly connected to the settling hopper 4 by a thread. The lower end of the settling hopper 4 is inserted into the separation box 5.
[0025] The separation box 5 has an insertion slot 7 on one side of its upper end. The initial selection mechanism is inserted into the upper end of the insertion slot 7. The separation box 5 at the lower end of the insertion slot 7 has an enrichment chamber 8. One end of the enrichment chamber 8 is connected to the airflow inlet 9, which is connected to the outside of one side of the separation box 5. The other end of the enrichment chamber 8 is connected to the gas delivery pipe 10. The end of the gas delivery pipe 10 is connected to the microfluidic chip 11, and the other end of the microfluidic chip 11 is connected to the outside of the other side of the separation box 5.
[0026] An exhaust fan is installed at the lower end of the air inlet 1. The exhaust fan is connected to the power supply and switch through a wire core. A threaded connector is installed at the lower end of the air inlet 1. The air inlet 1 is fixedly connected to the upper end of the primary selection cylinder 3 through the threaded connector. The air intake operation is achieved by using a low-power exhaust fan. The air containing spores can be drawn into the air inlet from the air inlet 2. The umbrella-shaped air collecting cap can effectively expand the air collecting surface and has a rainproof effect.
[0027] The lower end of the primary selection cylinder 3 is equipped with a filter structure, which is a filter screen. The filter screen is fixed to the inner wall of the lower end of the primary selection cylinder 3 by a fastener. A threaded joint is provided at the lower end of the primary selection cylinder 3. The primary selection cylinder 3 is fixed to the upper end of the settling hopper 4 by the threaded structure. Through the filter screen structure, the spore-containing gas entering the primary selection cylinder 3 can be initially filtered to remove large particulate impurities. The filtered spore-containing gas will settle under gravity in the settling hopper 4 and finally fall into the enrichment chamber 8.
[0028] The upper end of the separation box 5 is provided with an insertion slot 7 corresponding to the sedimentation hopper 4. The lower end of the insertion slot 7 is connected to the disc-shaped enrichment cavity 8. One side of the enrichment cavity 8 is connected to the airflow inlet 9, and the outside of the airflow inlet 9 is connected to the jet device. Through the enrichment cavity 8, the number of particles entering the microfluidic chip 11 region can be increased by gravity sedimentation, while reducing their speed.
[0029] The microfluidic chip 11 has a thickness of 3 mm and includes a spore airflow inlet 12 on one side. The spore airflow range is 5 mm / s to 30 mm / s, with a basic value of 5 mm / s. The spore airflow inlet 12 is connected to a microfluidic channel with a height of 70 μm. The end of the microfluidic channel is connected to a pretreatment zone 13. The pretreatment zone 13 is configured as a curved channel to form a filter impact plate structure. The end of the pretreatment zone 13 is connected to an axial focusing zone 14. The pretreatment zone 13 is used to initially screen the particles entering the fluid channel. Particles with greater inertia collide with and adhere to the impact plate, while smaller particles follow the airflow through the impactor, removing larger air aerosols and aggregated spores.
[0030] Therefore, the axial focusing zone 14 has a Y-shaped structure, including a microfluidic channel that connects to the curved channel of the pretreatment zone 13 in the middle, and sheath airflow channels that extend to both sides of the microfluidic channel. An axial sheath inlet hole is opened on the front of the end of the sheath airflow channel. The end of the microfluidic channel connects to the forward coupling separation zone 15. The flow velocity of the axial sheath flow is 10 mm / s, which is twice the airflow inlet velocity. The main function of the axial focusing zone 14 is to achieve the focusing of spores towards the center line of the microchannel by the squeezing effect of the double sheath airflow on the spore-containing airflow in the middle, so as to ensure that the spores move along the center line.
[0031] The orthogonal coupling separation zone 15 is a square impact area. The lower end of the impact area is connected to the sheath flow channel. The end of the sheath flow channel is provided with a radial sheath inlet hole. The end of the impact area is connected to the air outlet 16. The air outlet 16 is connected to the exhaust hole 6 opened on the side of the separation box 5. The flow velocity of the radial sheath flow is 20 mm / s, which is 4 times the air inlet velocity. The orthogonal coupling separation zone 15 is mainly used for the orthogonal coupling between the spores and the radial sheath flow after the spores are focused. Spores of different sizes are separated due to different forces and movement trajectories, thereby accurately separating virus spores of different diameters.
[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A microfluidic separation device for separating urediniospores, characterized in that: The utility model relates to a kind of microfluidic chip and microfluidic chip-based spore concentration device, including Primary election mechanism, the primary election mechanism includes air inlet end (1), the umbrella-shaped gas collection cap is provided on air inlet end (1) upper end, air inlet end (1) is opened with several ring array uniform distribution air inlet hole (2), the primary election cylinder (3) is fixedly connected to air inlet end (1) lower end by screw thread, the primary election cylinder (3) lower end is fixedly connected to sediment bucket (4) by screw thread, the sediment bucket (4) lower end is inserted into separation tank (5); The separation tank (5) is provided with an insertion slot (7) on one side of the upper end, and the primary election mechanism is inserted into the insertion slot (7) on the upper end. The separation tank (5) is provided with an enrichment chamber (8) inside the lower end of the insertion slot (7). One end of the enrichment chamber (8) is connected to an airflow inlet (9), and the airflow inlet (9) is connected to the outside of one side of the separation tank (5). The other end of the enrichment chamber (8) is connected to a gas delivery pipe (10), and the end of the gas delivery pipe (10) is connected to a microfluidic chip (11). The other end of the microfluidic chip (11) is connected to the outside of the other side of the separation tank (5).
2. The microfluidic separation device for separating urediniospores according to claim 1, characterized in that: An air extraction fan is arranged at the lower end inside the air inlet end (1). The air extraction fan is connected to a power source and a switch through a wire. A threaded joint is arranged at the lower end of the air inlet end (1). The air inlet end (1) is fixedly connected to the upper end of the primary election cylinder (3) through the threaded joint.
3. The microfluidic separation device for separating urediniospores according to claim 2, characterized in that: A filter structure is arranged inside the lower end of the primary election cylinder (3). The filter structure is a filter screen. The filter screen is fixed to the inner wall of the lower end of the primary election cylinder (3) through a fixing member. A threaded joint is arranged at the lower end of the primary election cylinder (3). The primary election cylinder (3) is fixed to the upper end of the sediment bucket (4) through the threaded structure.
4. The microfluidic separation device for separating urediniospores according to claim 1, wherein: The separation tank (5) is provided with an insertion slot (7) on the upper end, which corresponds to the sediment bucket (4). The insertion slot (7) is connected to a disc-shaped enrichment chamber (8) at the lower end. One side of the enrichment chamber (8) is connected to an airflow inlet (9). The outside of the airflow inlet (9) is connected to a gas injection device.
5. The microfluidic separation device for separating urediniospores according to claim 1, wherein: The thickness of the microfluidic chip is 3 mm. The microfluidic chip (11) includes a spore airflow inlet (12) on one side. The range of the spore airflow is 5 mm / s to 30 mm / s, and the basic value is 5 mm / s. The spore airflow inlet (12) is connected to a microfluidic channel. The height of the microfluidic channel is 70 um. The end of the microfluidic channel is connected to a pretreatment zone (13). The pretreatment zone (13) is arranged in a curved channel, forming a filter impact plate structure. The end of the pretreatment zone (13) is connected to an axial focusing zone (14).
6. The microfluidic separation device for separating urediniospores according to claim 5, characterized in that: Therefore, the axial focusing zone (14) is in a Y-shaped structure, including a microfluidic channel connected to the curved channel of the pretreatment zone (13) in the middle. On both sides of the microfluidic channel, sheath airflow channels are extended and connected. The end of each sheath airflow channel is provided with an axial sheath inlet hole in the front. The end of the microfluidic channel is connected to a forward coupling separation zone (15). The flow rate of the axial sheath flow is 10 mm / s, which is twice the speed of the airflow inlet.
7. The microfluidic separation device for separating urediniospores according to claim 6, characterized in that: The square impact area of the forward coupling separation area (15) is communicated with a sheath gas flow channel at the lower end of the impact area, a radial sheath inflow hole is arranged at the end of the sheath gas flow channel, and an outlet (16) is communicated with the end of the impact area. The outlet (16) is communicated with the exhaust hole (6) arranged on the side of the separation box (5), and the flow rate of the radial sheath flow is 20 mm / s, which is 4 times the velocity of the gas flow inlet.