Phage extraction device
By designing a phage extraction device that includes a culture container, calibration cap, rotating shaft, and adjustment components, the problem of difficult adjustment of the glass rod was solved, and efficient smoothing of the host bacteria and semi-solid agar mixture was achieved, thereby improving the phage extraction efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-09
- Publication Date
- 2026-04-14
AI Technical Summary
Existing glass rods are difficult to adjust when spreading the mixture of host bacteria and molten semi-solid agar, resulting in multiple smoothing operations, which affects the efficiency of phage culture and extraction.
A phage extraction device was designed, including a culture container, a calibration cap, a rotating shaft, an adjusting component, and a glass rod. By using the adjusting component in combination, the glass rod can be quickly adjusted and smoothed in all directions, avoiding smoothing dead corners and improving smoothing efficiency.
The device, through the design of the adjustment component, can quickly adjust the position of the glass rod to achieve all-round smoothing of the host bacteria and semi-solid agar mixture, thereby improving the efficiency of phage extraction and smoothing, and reducing unevenness.
Smart Images
Figure CN224118998U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of phage culture and extraction, and in particular to a phage extraction device. Background Technology
[0002] When extracting bacteriophages, the host bacteria (such as Escherichia coli) are mixed with melted semi-solid agar and spread evenly on the surface of solidified solid culture medium to form a double-layer plate. After the semi-solid agar solidifies, marked areas are delineated on the plate for subsequent spotting.
[0003] After the molten semi-solid agar and host are mixed and poured into the culture medium in the culture container, it needs to be leveled. The existing leveling method mainly uses a hook-shaped glass rod with a horizontal bottom, which is dipped in alcohol and sterilized by heating. The mixture of semi-solid agar and phage in the culture container is then leveled to facilitate the subsequent spotting of phage. The glass rod is rotated to achieve the leveling effect. However, the leveling end of the existing glass rod is difficult to adjust during use. Therefore, when leveling the culture medium and molten agar with the host bacteria, multiple leveling actions are required, which affects the phage culture itself and the efficiency of phage extraction. Utility Model Content
[0004] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a bacteriophage extraction device to solve the technical problems mentioned in the background art.
[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution:
[0006] A bacteriophage extraction device includes a culture container, a threaded portion on the top of the outer periphery of the culture container, a calibration cap threadedly connected to the top of the culture container via the threaded portion, a rotating shaft connected to the top of the calibration cap, the bottom end of the rotating shaft extending to the bottom of the calibration cap, and a glass rod connected to the bottom of the outer periphery of the rotating shaft via an adjusting member.
[0007] The adjusting component includes a support shaft, an annular groove, a cross hole, a threaded sleeve, an adjusting tube, and a cross rod. The support shaft is horizontally fixedly connected to the outer periphery of the bottom end of the rotating shaft. The annular groove is formed at the end of the support shaft away from the rotating shaft. The cross hole is formed at the center of the end of the support shaft away from the rotating shaft. The threaded sleeve is rotatably connected to the end of the support shaft away from the rotating shaft. The adjusting tube is threadedly connected to the threaded sleeve, and its end extends into the annular groove. The end of the adjusting tube away from the support shaft is closed. The cross rod is fixedly connected to the end of the adjusting tube away from the support shaft, and its end extends into the cross hole. The glass rod is horizontally positioned below the adjusting tube, and its end is connected to the end of the adjusting tube away from the support shaft.
[0008] In a preferred embodiment, the present invention can be further configured such that the glass rod is L-shaped, and the outer wall of the glass rod is in contact with the bottom and side walls of the culture container.
[0009] In a preferred embodiment, the present invention can be further configured such that the end of the glass rod is hook-shaped and the length of the glass rod is greater than the radius of the inner bottom sidewall of the culture container.
[0010] In a preferred embodiment, the present invention can be further configured such that: a positioning ring is rotatably connected to the outer circumference of the rotating shaft, and the positioning ring is threadedly connected to the middle of the top side of the calibration cover.
[0011] In a preferred embodiment, the present invention can be further configured such that: an injection hole is provided on the top side of the calibration cover, and a sealing plug is inserted into the injection hole.
[0012] In a preferred embodiment, the present invention can be further configured such that the top end of the rotating shaft extends above the positioning ring, and a rotating handle is connected to the top end of the rotating shaft.
[0013] In summary, this utility model has at least one of the following beneficial technical effects:
[0014] 1. The phage extraction device allows for rapid adjustment of the glass rod position using adjustable components during use. This enables the glass rod to smooth the host bacteria, semi-solid agar mixture, and culture medium solution from all angles during rotation, facilitating subsequent phage spotting and extraction and improving the efficiency of phage extraction.
[0015] 2. The phage extraction device has a rotating shaft whose top end extends to the top of the positioning ring and is connected to a rotating handle. Twisting the rotating handle will drive the rotating shaft to rotate. With the position limitation of the calibration cover, it can directly and quickly smooth the culture medium and the mixture of host bacteria and semi-solid agar in the culture container from all directions, thereby improving the smoothing efficiency.
[0016] 3. In this phage extraction device, the end of the glass rod is hook-shaped. The rounded end formed by the hook shape prevents the edge of the glass rod from scratching the mixture of host bacteria and semi-solid agar or culture medium, thus avoiding unevenness. In addition, the length of the glass rod is greater than the radius of the bottom side wall of the culture container to avoid dead corners in the smoothing process, which would affect the smoothing work. Attached Figure Description
[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0018] Figure 1 This is a schematic diagram of the overall structure of a bacteriophage extraction device according to the present invention.
[0019] Figure 2 This is a schematic diagram of the internal structure of the culture container of a bacteriophage extraction device according to the present invention.
[0020] Figure 3 This is a schematic diagram of the adjusting component structure of a bacteriophage extraction device according to the present invention.
[0021] Figure 4 This is a schematic diagram of the calibration cap structure of a bacteriophage extraction device according to the present invention.
[0022] In the diagram, 1. Culture container; 2. Threaded part; 3. Calibration cover; 4. Rotating shaft; 5. Glass rod; 6. Adjusting component; 7. Support shaft; 8. Annular groove; 9. Cross hole; 10. Threaded sleeve; 11. Adjusting tube; 12. Cross rod; 13. Positioning ring; 14. Injection hole; 15. Sealing plug; 16. Rotating handle. Detailed Implementation
[0023] The present invention will be further described in detail below with reference to the accompanying drawings.
[0024] Example:
[0025] Reference Figures 1-4The present invention discloses a bacteriophage extraction device, including a culture container 1, a threaded part 2 provided on the top of the outer periphery of the culture container 1, a calibration cover 3 threadedly connected to the top of the culture container 1 through the threaded part 2, a rotating shaft 4 connected to the top of the calibration cover 3, the bottom end of the rotating shaft 4 extending to the bottom of the calibration cover 3, and a glass rod 5 connected to the bottom of the outer periphery of the rotating shaft 4 through an adjusting member 6.
[0026] The adjusting component 6 includes a support shaft 7, an annular groove 8, a cross hole 9, a threaded sleeve 10, an adjusting tube 11, and a cross rod 12. The support shaft 7 is horizontally fixedly connected to the outer periphery of the bottom end of the rotating shaft 4. The annular groove 8 is opened at the end of the support shaft 7 away from the rotating shaft 4. The cross hole 9 is opened at the center of the end of the support shaft 7 away from the rotating shaft 4. The threaded sleeve 10 is rotatably connected to the end of the support shaft 7 away from the rotating shaft 4. The adjusting tube 11 is threadedly connected to the threaded sleeve 10, and its end extends into the annular groove 8. The end of the adjusting tube 11 away from the support shaft 7 is closed. The cross rod 12 is fixedly connected to the end of the adjusting tube 11 away from the support shaft 7. The end of the cross rod 12 extends into the cross hole 9. The glass rod 5 is horizontally arranged below the adjusting tube 11, and its end is connected to the end of the adjusting tube 11 away from the support shaft 7.
[0027] In this embodiment, reference Figure 1 When using, pour the culture medium into culture container 1, as per the instructions. Figure 2 The calibration cap 3 is threaded into the culture container 1 using the threaded part 2. When the calibration cap 3 is threaded into the culture container 1, the calibration cap 3 rotates on the threaded part 2, which will cause the glass rod 5 to descend. As the glass rod 5 descends, it will gradually approach the inner bottom side wall of the culture container 1. After the glass rod 5 moves to the designated position, the rotation of the calibration cap 3 is stopped. Then the rotating shaft 4 is rotated. The rotating shaft 4 drives the support shaft 7, the threaded sleeve 10 and the adjusting tube 11 to make circular motion, which in turn drives the glass rod to make circular motion, thereby smoothing the culture medium in the culture container 1, so as to smooth it when the host bacteria and semi-solid agar mixture is injected into the top of the culture medium.
[0028] When dealing with culture containers 1 of different diameters, refer to Figure 3The threaded sleeve 10 is rotated to drive the adjustment tube 11 to move, so that the adjustment tube 11 can drive the glass rod 5 to move. After the glass rod 5 is moved to the appropriate position, the rotation of the threaded sleeve 10 is stopped, so that when the rotating shaft 4 is rotated, the glass rod 5 can quickly smooth the host bacteria and semi-solid agar mixture on the top surface of the culture medium. The cross rod 12 and the cross hole 9 are set to limit the adjustment tube 11 and prevent it from rotating with the threaded sleeve 10, while the annular groove 8 is set to avoid the movement of the adjustment tube 11.
[0029] After the culture medium solidifies, remove the calibration cap 3, place the host bacteria and semi-solid agar mixture into the mixture, and then replace the calibration cap 3. At the same time, move the glass rod 5 to the designated position and rotate the rotating shaft 4. The rotating shaft 4 drives the support shaft 7, the adjusting tube 11, and the glass rod 5 to rotate, directly smoothing the host bacteria and semi-solid agar mixture to facilitate subsequent spotting and phage extraction.
[0030] In a further preferred embodiment of this utility model, such as Figure 2 As shown, the glass rod 5 is L-shaped, and the outer wall of the glass rod 5 is attached to the bottom side wall and the side wall of the culture container 1.
[0031] In this embodiment, reference Figure 2 The glass rod 5 is L-shaped, which allows the outer wall of the glass rod 5 to fit against the bottom and inner circumference of the culture container 1, thereby facilitating the smoothing of the culture medium at the bottom of the culture container 1 and the subsequent mixture of host bacteria and semi-solid agar.
[0032] In a further preferred embodiment of this utility model, such as Figure 2 As shown, the end of the glass rod 5 is hook-shaped, and the length of the glass rod 5 is greater than the radius of the inner bottom sidewall of the culture container 1.
[0033] In this embodiment, reference Figure 2 The glass rod 5 has a hook-shaped end. The rounded end of the hook-shaped end prevents the edge of the glass rod 5 from scratching the mixture of host bacteria and semi-solid agar or culture medium, thus avoiding unevenness. In addition, the length of the glass rod 5 is greater than the radius of the inner bottom side wall of the culture container 1 to avoid dead corners in the smoothing process and affect the smoothing work.
[0034] In a further preferred embodiment of this utility model, such as Figure 2 As shown, a positioning ring 13 is rotatably connected to the outer circumference of the rotating shaft 4, and the positioning ring 13 is threadedly connected to the middle of the top side of the calibration cover 3.
[0035] In this embodiment, reference Figure 2The positioning ring 13 is threaded to the outer circumference of the rotating shaft 4 and is rotatably connected to the top center of the calibration cover 3. When adjusting the longitudinal position of the glass rod 5, the positioning ring 13 can be directly rotated, which will drive the rotating shaft 4 to move up and down to adjust the longitudinal position of the glass rod 5.
[0036] In a further preferred embodiment of this utility model, such as Figure 2 and Figure 4 As shown, an injection hole 14 is provided on the top side of the calibration cover 3, and a sealing plug 15 is inserted into the injection hole 14.
[0037] In this embodiment, reference Figure 2 and Figure 4 The injection hole 14 is used to inject the host bacteria and semi-solid agar mixture and culture medium solution into the culture container 1, so that the host bacteria and semi-solid agar mixture or culture medium in the culture container 1 have less contact with the outside air, thereby enabling rapid smoothing and reducing the influence of the external environment on the bacteriophage.
[0038] In a further preferred embodiment of this utility model, such as Figure 2 As shown, the top end of the rotating shaft 4 extends above the positioning ring 13, and a rotating handle 16 is connected to the top end of the rotating shaft 4.
[0039] In this embodiment, reference Figure 2 The rotating shaft 4 extends to the top of the positioning ring 13 and is connected to the rotating handle 16. Twisting the rotating handle 16 will drive the rotating shaft 4 to rotate. With the position limitation of the calibration cover 3, it can directly and quickly smooth the culture medium and the mixture of host bacteria and semi-solid agar in the culture container 1 from all directions, thus improving the smoothing efficiency.
[0040] The implementation principle of the above embodiment is as follows: When dealing with culture containers 1 of different diameters, the rotating threaded sleeve 10 drives the movement of the adjusting tube 11, so that the adjusting tube 11 can drive the glass rod 5 to move. After the glass rod 5 is moved to a suitable position, the rotating threaded sleeve 10 is stopped, so that when the rotating shaft 4 is rotated, the glass rod 5 can quickly smooth the host bacteria and semi-solid agar mixture on the top surface of the culture medium. The cross rod 12 and the cross hole 9 are set to limit the adjusting tube 11 and prevent it from rotating with the threaded sleeve 10, while the annular groove 8 is set to avoid the movement of the adjusting tube 11.
[0041] The embodiments described herein are preferred embodiments of this utility model and are not intended to limit the scope of protection of this utility model. Therefore, all equivalent changes made to the structure, shape, and principle of this utility model should be included within the scope of protection of this utility model.
Claims
1. A bacteriophage extraction device, comprising a culture container, characterized in that, The top of the outer periphery of the culture container is provided with a threaded part, and the top of the culture container is threadedly connected to a calibration cover through the threaded part. The top of the calibration cover is connected to a rotating shaft, the bottom end of the rotating shaft extends to the bottom of the calibration cover, and the bottom of the outer periphery of the rotating shaft is connected to a glass rod through an adjusting component. The adjusting component includes a support shaft, an annular groove, a cross hole, a threaded sleeve, an adjusting tube, and a cross rod. The support shaft is horizontally fixedly connected to the outer periphery of the bottom end of the rotating shaft. The annular groove is formed at the end of the support shaft away from the rotating shaft. The cross hole is formed at the center of the end of the support shaft away from the rotating shaft. The threaded sleeve is rotatably connected to the end of the support shaft away from the rotating shaft. The adjusting tube is threadedly connected to the threaded sleeve, and its end extends into the annular groove. The end of the adjusting tube away from the support shaft is closed. The cross rod is fixedly connected to the end of the adjusting tube away from the support shaft, and its end extends into the cross hole. The glass rod is horizontally positioned below the adjusting tube, and its end is connected to the end of the adjusting tube away from the support shaft.
2. The phage extraction device according to claim 1, characterized in that, The glass rod is L-shaped, and its outer sidewall is in contact with the bottom and sidewalls of the culture container.
3. The phage extraction device according to claim 2, characterized in that, The end of the glass rod is hook-shaped, and the length of the glass rod is greater than the radius of the inner bottom sidewall of the culture container.
4. The phage extraction device according to claim 3, characterized in that, A positioning ring is rotatably connected to the outer circumference of the rotating shaft, and the positioning ring is threadedly connected to the middle of the top side of the calibration cover.
5. The phage extraction device according to claim 4, characterized in that, An injection hole is provided on the top side of the calibration cover, and a sealing plug is inserted into the injection hole.
6. The phage extraction device according to claim 5, characterized in that, The top end of the rotating shaft extends above the positioning ring, and a rotating handle is connected to the top end of the rotating shaft.