Reactor capable of storing liquid and used for staged plant culture

By designing a reactor for staged plant cultivation with liquid storage, the problems of high agar consumption and high risk of contamination in solid culture methods were solved, enabling precise management of the culture medium and long-term aseptic culture, thereby improving the efficiency and stress resistance of plant cultivation.

CN224124866UActive Publication Date: 2026-04-17JIUJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
JIUJIANG UNIV
Filing Date
2025-05-23
Publication Date
2026-04-17

AI Technical Summary

Technical Problem

Existing plant culture reactors use solid culture methods, which result in high agar consumption, complex operation, high risk of contamination, and difficulty in achieving precise phased management of the culture medium.

Method used

Design a reactor for staged plant culture with liquid storage, comprising a tank body, a tank cover, a classification culture component and a filtration and sterilization component. It can store multiple culture solutions and achieve uniform dispersion of the culture solutions through water guide baffles and water distribution plates. Air quality is controlled by an air pump and an air filter to support long-term aseptic culture.

Benefits of technology

It enables precise control of the plant cultivation process, reduces the risk of contamination, enhances the plant's stress resistance, improves cultivation efficiency and flexibility, and supports long-term cultivation without the need to change the culture medium.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model belongs to the technical field of plant biology, and particularly relates to a reactor capable of storing liquid and used for plant cultivation in stages, which comprises a tank body, a tank cover is mounted at the top of the tank body, and a plurality of tank built-in threaded holes are formed in the top of the tank body. The tank cover is provided with a plurality of cover built-in threaded holes used in cooperation with the tank body built-in threaded holes, each tank body built-in threaded hole and the corresponding cover built-in threaded hole are internally connected with sealing screws in a threaded mode, and a classified culture assembly is arranged in the tank body. The classified culture assembly comprises a water guide partition plate fixedly connected to an inner cavity of the tank body. According to the utility model, culture solutions with various formulas can be stored, the accurate control on the plant culture process is realized, the requirements of each stage of plant growth are met, the long-term use is supported, the culture solutions do not need to be frequently replaced, and the pollution problem in the tissue culture process is effectively controlled; and a ventilation facility is arranged, so that the stress resistance of the plants is enhanced, and a solid guarantee is provided for subsequent seedling hardening operation.
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Description

Technical Field

[0001] This utility model belongs to the field of plant biotechnology, and specifically relates to a reactor for staged plant cultivation with liquid storage. Background Technology

[0002] A plant culture reactor is a bioreactor used for the culture of plant cells, tissues, or organs. It can simulate the environmental conditions of plants at different growth stages, providing precise control and optimization for plant culture to achieve efficient and large-scale plant culture. Staged plant culture reactors provide suitable growth conditions for plant cells, tissues, or organs by precisely controlling various parameters in the culture environment, such as temperature, light, humidity, gas composition, and nutrient supply. At different culture stages, these parameters can be automatically adjusted according to the growth needs of the plant to promote plant growth, development, and the synthesis of metabolites.

[0003] Currently, most plant culture reactors on the market use solid culture methods, with agar in culture bottles as the culture medium. However, this method consumes a large amount of agar, and numerous containers need to be handled during filling and cleaning, which undoubtedly increases the complexity and labor intensity of the operation. Solid culture is cumbersome to change the culture medium, making it difficult to achieve precise management of the culture medium in stages. If not cleaned properly, there is a risk of cross-contamination, which to some extent limits the efficiency and quality of plant culture. Utility Model Content

[0004] The purpose of this invention is to provide a reactor for staged plant culture with liquid storage, capable of storing culture solutions of various formulations and achieving precise control over the plant culture process to meet the needs of each stage of plant growth. At the same time, it supports long-term use without frequent replacement of culture solutions, effectively controlling pollution problems in the tissue culture process. It also has ventilation facilities, which significantly enhances the plant's stress resistance and provides a solid guarantee for subsequent hardening-off operations.

[0005] The specific technical solution adopted by this utility model is as follows:

[0006] A reactor for staged plant cultivation with liquid storage includes a tank body, a tank cover installed on the top of the tank body, a plurality of internal threaded holes in the top of the tank body, a plurality of internal threaded holes in the tank cover that cooperate with the internal threaded holes in the tank body, a sealing screw threadedly connected to each internal threaded hole in the tank body and the internal threaded hole in the cover, and a classification culture component disposed inside the tank body.

[0007] Preferably, the classification culture component includes a water-guiding baffle fixedly connected to the inner cavity of the tank body, a water-dividing plate fixedly connected to the inner wall of the tank body and at the top of the water-guiding baffle, a tank sealing ring fixedly connected to the inner wall of the tank body and at the bottom of the water-guiding baffle, four tank baffles arranged in a circumferential array fixedly connected to the inner wall of the tank body and at the bottom of the tank sealing ring, multiple water-guiding pipes connected around the bottom of the water-guiding baffle, and a filtration and sterilization component connected to the surface of the tank body.

[0008] Preferably, the filtration and sterilization assembly includes a first tank connector connected to the surface of the tank body and near the bottom of the tank lid. A first air filter is connected to one side of the first tank connector. Multiple second tank connectors are connected to the surface of the tank body and around the bottom of the water guide baffle. An air valve switch is fixedly installed at one end of each second tank connector. The end of the air valve switch away from the second tank connector is connected to a second air filter. An air pump is installed on one side of the tank body. The output end of the air pump is connected to a silicone hose, and one end of the silicone hose is connected to the second air filter. A timer is electrically connected to one side of the air pump.

[0009] Preferably, a grid is fixedly connected to the inner wall of the tank body and to the top of the water distribution plate.

[0010] Preferably, a plurality of tank sealing buckles are fixedly installed on the inner wall of the tank body and around the top of the water guide baffle, and each of the tank sealing buckles is triangular in shape.

[0011] Preferably, a lid sealing ring is fixedly connected to the bottom of the can lid, and the surface of the lid sealing ring is in contact with the top of the inner cavity of the can body.

[0012] The technical effects achieved by this utility model are as follows:

[0013] This invention relates to a reactor for staged plant cultivation with liquid storage. It includes a categorized cultivation component. Plant material is placed on a water-guiding baffle. Cultivation solution is then injected into four spaces at the bottom of the tank body, separated by baffles. A perforated plate evenly distributes the cultivation solution from the water-guiding pipes at the bottom of the baffles, ensuring direct contact with the plant material and avoiding direct impact. This guarantees uniform nutrient absorption and a favorable growth environment, improving cultivation efficiency and flexibility, and preventing contamination. A first air filter and a first tank connection pipe are combined to filter air, maintaining sterile conditions within the tank. A second air filter... The second tank connection ensures sterility of the air in the culture medium area, reducing the risk of internal contamination. The air pump is controlled by a timer to adjust the immersion culture cycle. A silicone hose connects the air pump and filter, and an air valve regulates the air flow to ensure that air enters and exits the tank body at a predetermined frequency. This device can store culture media with various formulations and achieve precise control of the plant culture process, meeting the needs of each stage of plant growth. It also supports long-term use without frequent culture medium changes, effectively controlling contamination during tissue culture. The ventilation system significantly enhances the plant's resistance to stress, providing a solid guarantee for subsequent hardening-off operations. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the main structure of this utility model;

[0015] Figure 2 This is a three-dimensional structural view of the classification and cultivation component of this utility model;

[0016] Figure 3 This is a three-dimensional structural view of the filtration and sterilization component of this utility model;

[0017] Figure 4 This is a structural cross-sectional view of the tank body of this utility model.

[0018] The attached diagram lists the components represented by each number as follows:

[0019] 1. Tank body; 2. Tank lid; 3. Internal threaded hole in the tank body; 4. Internal threaded hole in the lid; 5. Sealing screw; 6. Water guide baffle; 7. Water distribution plate; 8. Tank sealing ring; 9. Tank baffle; 10. Water guide pipe; 11. First tank connecting pipe; 12. First air filter; 13. Second tank connecting pipe; 14. Air valve switch; 15. Second air filter; 16. Air pump; 17. Silicone hose; 18. Timer; 19. Grid; 20. Tank sealing buckle; 21. Lid sealing ring. Detailed Implementation

[0020] To make the objectives and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific embodiments of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0021] like Figures 1-4 As shown, a reactor for staged plant cultivation with liquid storage includes a tank body 1, a tank cover 2 installed on the top of the tank body 1, a plurality of internal threaded holes 3 on the top of the tank body 1, a plurality of internal threaded holes 4 for the cover to cooperate with the internal threaded holes 3, a sealing screw 5 threadedly connected to each internal threaded hole 3 and the internal threaded hole 4, and a classification culture component is provided inside the tank body 1.

[0022] The tank body 1 is designed to cultivate plant materials and hold nutrient solutions. The tank lid 2 is made of transparent material to facilitate light exposure for the plant materials. After the threaded hole 3 inside the tank body 1 aligns with the threaded hole 4 inside the lid 2, a sealing screw 5 secures the connection, ensuring a tight seal. The categorized cultivation component uses a partition 9 to divide the interior of the tank body 1, allowing for the storage of different nutrient solutions and controlled cultivation to meet the needs of different stages of plant cultivation. The design of the water-guiding partition 6 and water-guiding pipe 10 addresses the issue of contamination that can easily occur when changing the nutrient solution during the cultivation process.

[0023] like Figure 2 and Figure 3 As shown, the classification culture component includes a water guide baffle 6 fixedly connected to the inner cavity of the tank body 1, a water distribution plate 7 fixedly connected to the inner wall of the tank body 1 and at the top of the water guide baffle 6, a tank sealing ring 8 fixedly connected to the inner wall of the tank body 1 and at the bottom of the water guide baffle 6, four tank baffles 9 arranged in a circular array fixedly connected to the inner wall of the tank body 1 and at the bottom of the tank sealing ring 8, multiple water guide pipes 10 connected around the bottom of the water guide baffle 6, and a filter sterilization component connected to the surface of the tank body 1.

[0024] Specifically, the water-guiding baffle 6 is used to divide the interior of the tank body 1 into two parts: the upper part is for the cultivation of plant materials, and the lower part is for the storage of culture medium. The water-guiding pipe 10 is used to guide the culture medium to the upper part for cultivating plant materials. The water-distributing plate 7 is used to evenly disperse the culture medium discharged from the water-guiding pipe 10 at the bottom of the water-guiding baffle 6, preventing the discharged culture medium from impacting the plant materials and causing accumulation, which would affect plant growth. The tank body baffle 9 can evenly divide the lower part of the tank body into four parts, so that the bottom cavity of the tank body 1 has four spaces for storing liquid. Different types of culture medium can be stored in the four spaces to meet the requirements of different culture media at different stages of plant growth or the different cultivation conditions at different stages of plant growth. Different types of culture media can also be set up as needed. The system allows for the selection of different culture medium types to meet the needs of single or several stages of cultivation. The selected culture medium is introduced from the lower culture medium compartment (10) to the upper plant material compartment (6) via the water guide pipe 10. During this process, the design of the water distribution plate 7 ensures uniform dispersion of the culture medium, avoiding accumulation caused by direct impact on the plant material and guaranteeing uniform hydration and a suitable growth environment. Subsequently, as plant cultivation progresses, the culture medium type in the four spaces separated by the tank partition 9 at the bottom of the tank body 1 can be changed or adjusted according to the different growth stages of the plants. This satisfies the specific nutritional and environmental requirements of the plants at different growth stages, improving the efficiency and flexibility of plant cultivation and effectively avoiding the risk of contamination during the cultivation process.

[0025] like Figure 2 and Figure 3 As shown, the filtration and sterilization assembly includes a first tank pipe 11 connected to the surface of the tank body 1 and near the bottom of the tank cover 2. A first air filter 12 is connected to one side of the first tank pipe 11. Multiple second tank pipes 13 are connected to the surface of the tank body 1 and around the bottom of the water guide baffle 6. An air valve switch 14 is fixedly installed at one end of each second tank pipe 13. The end of the air valve switch 14 away from the second tank pipe 13 is connected to a second air filter 15. An air pump 16 is installed on one side of the tank body 1. The output end of the air pump 16 is connected to a silicone hose 17, and one end of the silicone hose 17 is connected to the second air filter 15. A time controller 18 is electrically connected to one side of the air pump 16.

[0026] The first air filter 12 is used to filter air, ensuring that the air entering and exiting the tank body 1 meets the sterility standard. The first tank connector 11 works in conjunction with the first air filter 12 to ensure air filtration and sterilization in the upper plant culture material area, effectively preventing microorganisms in the air from invading the culture environment, thereby maintaining the sterile conditions for plant culture. The second air filter 15 also has the function of filtering air, ensuring that the air in the lower part of the tank body 1 where the culture medium is placed is sterile, reducing the possibility of the culture medium being contaminated. The second tank connector 13 is responsible for connecting the second air filter 15 to the tank body 1. The air pump 16 provides the air pressure required for operation. The timer 18 operates the air pump 16 by controlling the on and off of the current, and controls the immersion culture cycle of the device by adjusting the on and off frequency of the current. The silicone hose 17 is used to connect the air pump 16 to the second air filter 15. The air valve switch 14 is used to adjust the air flow rate entering and exiting the tank body 1, ensuring that air can enter or exit the tank body 1 at a predetermined frequency and time interval during the immersion culture and aeration culture stages.

[0027] like Figure 3 and Figure 4 As shown, a grid 19 is fixedly connected to the inner wall of the tank body 1 and to the top of the water distribution plate 7.

[0028] Specifically, the grid 19 can further disperse the culture solution dispersed by the water distribution plate 7, ensuring that the plant material can fully absorb the nutrients in the culture solution and promote the healthy growth of the plant; at the same time, it can prevent the plant material from falling below the tank body 1.

[0029] like Figure 2 As shown, multiple tank sealing buckles 20 are fixedly installed on the inner wall of the tank body 1 and around the top of the water guide baffle 6. Each tank sealing buckle 20 has a triangular structure.

[0030] The tank sealing buckle 20 is used to limit and engage the water guide baffle 6, ensuring that the water guide baffle 6 is close to the tank sealing ring 8 and the tank baffle 9, so that the tank sealing ring 8 can seal the water guide baffle 6 with the protruding part of the inner wall of the tank body 1 and the tank baffle 9.

[0031] like Figure 1 As shown, a lid sealing ring 21 is fixedly connected to the bottom of the lid 2, and the surface of the lid sealing ring 21 is in contact with the top of the inner cavity of the can body 1.

[0032] Specifically, the lid sealing ring 21 is made of high-temperature and high-pressure resistant materials such as silicone to ensure the sealing effect between the lid sealing ring 21 and the top of the inner cavity of the can body 1, ensuring the sterility of the can body 1 and providing a reliable guarantee for the healthy growth of plant materials.

[0033] The working principle of this utility model is as follows: First, the plant material is placed on the water-guiding baffle 6. Then, the culture medium is injected into the four spaces separated by the tank baffle 9 at the bottom of the tank body 1. The culture medium is guided to the upper part through the water-guiding pipe 10 for culturing the plant material. The water-dividing plate 7 is used to evenly disperse the culture medium discharged from the water-guiding pipe 10 at the bottom of the water-guiding baffle 6, so that it comes into direct contact with the plant material. After a period of cultivation, if it is necessary to continue to use the remaining culture medium in the tank body 1 for cultivation, the second air filter 15 can be replaced and the corresponding air valve switch 14 can be opened, while the original air valve switch 14 is closed. This ensures that only one of the four air valve switches 14 in the lower part of the tank body 1 is always open. Then, cultivation continues, thus completing the cultivation of plants with a sufficient amount of culture medium for a long time.

[0034] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A reactor for the cultivation of plants in stages with liquid storage, characterized in that: The container includes a tank body (1), a tank cover (2) is installed on the top of the tank body (1), the top of the tank body (1) is provided with a plurality of internal threaded holes (3), the tank cover (2) is provided with a plurality of internal threaded holes (4) that cooperate with the internal threaded holes (3), and each internal threaded hole (3) and internal threaded hole (4) is threaded with a sealing screw (5), and a classification culture component is provided inside the tank body (1).

2. The reactor according to claim 1, wherein: The classification and culture component includes a water guide baffle (6) fixedly connected to the inner cavity of the tank body (1), a water distribution plate (7) fixedly connected to the inner wall of the tank body (1) and the top of the water guide baffle (6), a tank sealing ring (8) fixedly connected to the inner wall of the tank body (1) and the bottom of the water guide baffle (6), four tank baffles (9) arranged in a circular array fixedly connected to the inner wall of the tank body (1) and the bottom of the tank sealing ring (8), multiple water guide pipes (10) connected around the bottom of the water guide baffle (6), and a filter sterilization component connected to the surface of the tank body (1).

3. The reactor according to claim 2, wherein: The filtration and sterilization assembly includes a first tank connector (11) connected to the surface of the tank body (1) and near the bottom of the tank cover (2). A first air filter (12) is connected to one side of the first tank connector (11). Multiple second tank connectors (13) are connected to the surface of the tank body (1) and around the bottom of the water guide baffle (6). An air valve switch (14) is fixedly installed at one end of each second tank connector (13). The end of the air valve switch (14) away from the second tank connector (13) is connected to a second air filter (15). An air pump (16) is installed on one side of the tank body (1). The output end of the air pump (16) is connected to a silicone hose (17), and one end of the silicone hose (17) is connected to the second air filter (15). A time controller (18) is electrically connected to one side of the air pump (16).

4. The reactor according to claim 1, wherein: A grid (19) is fixedly connected to the inner wall of the tank body (1) and to the top of the water distribution plate (7).

5. The reactor according to claim 2, wherein: Multiple tank sealing buckles (20) are fixedly installed on the inner wall of the tank body (1) and around the top of the water guide baffle (6). Each tank sealing buckle (20) has a triangular structure.

6. The reactor according to claim 1, wherein: The bottom of the can lid (2) is fixedly connected to a lid sealing ring (21), and the surface of the lid sealing ring (21) is in contact with the top of the inner cavity of the can body (1).