Carbon dioxide proportioning device

Abstract

The utility model discloses a carbon dioxide proportioning device, which relates to the technical field of gas proportioning, and comprises two gas input mechanisms arranged in parallel, the output ends of the two gas input mechanisms are both connected to the same gas storage tank, the input end of one gas input mechanism is connected with a gas source only storing carbon dioxide, and the output end of the other gas input mechanism is connected with a gas pump. The input end of one gas input mechanism is arranged in the air, the input end of the other gas input mechanism is arranged in the air, the two gas input mechanisms are driven by the same driving device, the air and the carbon dioxide are synchronously and quantitatively input into the gas storage tank for multiple times, and the amount of the air and the amount of the carbon dioxide input each time are adjustable. According to the utility model, synchronous and multi-time quantitative input of air and carbon dioxide is realized, the carbon dioxide requirement of mesenchymal stem cell balling culture can be accurately matched, the gas proportioning efficiency and stability are remarkably improved while the gas circuit structure is simplified and the equipment cost is reduced, and the cell ball microenvironment uniformity is ensured.

Description

Technical Field

[0001] This utility model relates to the field of gas proportioning technology, specifically a carbon dioxide proportioning device. Background Technology

[0002] During the spheroidization culture of mesenchymal stem cells, a quantitative concentration of carbon dioxide is required to maintain a suitable culture environment. Current technologies typically employ a single gas source (such as a high-pressure CO2 cylinder) mixed with air before being introduced into the incubator. However, this method relies on the coordinated operation of various sensors and solenoid valves to achieve precise carbon dioxide concentration control. This approach is not only structurally complex but also incurs high production and operating costs, leading to numerous inconveniences in practical applications. Furthermore, existing technologies lack a device capable of simultaneously and repeatedly quantitatively introducing air and carbon dioxide, failing to meet the requirements for efficient and precise gas mixing. Therefore, improvements to existing technologies are urgently needed to address these issues. Utility Model Content

[0003] The purpose of this invention is to provide a carbon dioxide proportioning device to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution:

[0005] A carbon dioxide proportioning device includes two parallel gas input mechanisms, the output ends of which are connected to the same gas storage tank. The input end of one gas input mechanism is connected to a gas source that stores only carbon dioxide, while the input end of the other gas input mechanism is placed in the air. The two gas input mechanisms are driven by the same drive device to synchronously and quantitatively input air and carbon dioxide into the gas storage tank multiple times. The amount of air and carbon dioxide input in each instance is adjustable.

[0006] Preferably, the gas input mechanism includes a fixed mixing cylinder and a piston disposed inside the mixing cylinder that can move along the axial direction of the mixing cylinder. The piston is driven by a power mechanism to reciprocate in the mixing cylinder. A three-way pipe is fixed at the opening of the mixing cylinder, and a one-way valve is fixed on both ends of the three-way pipe. The one-way valve makes one end of the three-way pipe an input end and the other end an output end. As the piston reciprocates in the mixing cylinder, gas is drawn and sent into the gas storage tank.

[0007] Preferably, the power mechanisms of the two gas input mechanisms are connected in series, with one power mechanism being driven by a drive motor to rotate, thereby causing the two power mechanisms to rotate synchronously.

[0008] Preferably, the power mechanism includes a U-shaped rotating crank, a connecting rod, and a piston rod. Each of the two feet of the rotating crank is equipped with an adjustable transmission shaft. The axis of the transmission shaft is perpendicular to the axis of the foot of the rotating crank, and the fixed end of the transmission shaft is circularly connected to the foot of the rotating crank, while the movable end rotates around the circumference of the fixed end. The rotating cranks are connected in series via the transmission shafts and rotate synchronously. The rotating cranks rotate around the axis of the transmission shaft. The middle part of the rotating crank is rotatably connected to one end of the connecting rod, and the other end of the connecting rod is rotatably connected to one end of the piston rod. One end of the piston rod is fixedly connected to a piston. When the rotating cranks rotate, the piston in the proportioning cylinder is driven to reciprocate sequentially through the connecting rod and the piston rod.

[0009] Preferably, all the drive shafts are coaxially arranged, with the two drive shafts furthest apart being rotatably connected to a bearing seat, and one end of the two drive shafts being connected to the drive shaft of a fixedly installed drive motor. The movable end of the drive shaft between the two rotating cranks abuts and is fixed, and the drive motor drives all the rotating cranks to rotate through the drive shaft.

[0010] Preferably, both feet of the rotating crank are provided with external threads, and the fixed end of the transmission shaft has a fixed cylinder fixedly connected to the transmission shaft. A threaded cylinder is rotatably connected inside the fixed cylinder. The threaded cylinder has an internal thread that matches the external thread on the feet of the rotating crank. By rotating the threaded cylinder, the position of the threaded cylinder on the feet of the rotating crank can be adjusted.

[0011] Preferably, the movable end of the drive shaft is fixed with a detachable connecting flange, and the connecting flanges are fixed together by bolts to realize the connection between the drive shafts.

[0012] Preferably, the fixed cylinder is provided with a set screw for fixing the threaded cylinder and the fixed cylinder. Tightening the set screw can realize the synchronous rotation of the fixed cylinder and the threaded cylinder. At this time, the threaded cylinder can be rotated through the transmission shaft, thereby adjusting its position on the rotating crank foot.

[0013] Preferably, the two feet of the rotating crank are provided with grooves extending along the axis of the feet, and the grooves are provided with scale lines, which can be used as a reference when adjusting the position of the threaded cylinder.

[0014] Preferably, the connecting rod is provided with a connecting ring at one end of the rotating crank, and the inner diameter of the connecting ring is the same as the shaft diameter at the middle of the rotating crank; the connecting ring includes two opposing semicircular rings, one of which is fixedly connected to the connecting rod, and one end of the other semicircular ring is hinged to one end of the aforementioned semicircular ring by a hinge, and the other end is fastened together by a J-shaped or U-shaped connector, with the two legs of the connector passing through the two semicircular rings in a direction parallel to the axis of the connecting ring.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] This invention enables the simultaneous and multiple quantitative input of air and carbon dioxide, which can precisely match the carbon dioxide requirements of mesenchymal stem cell spheroid culture. While simplifying the gas path structure and reducing equipment costs, it significantly improves the efficiency and stability of gas ratio and ensures the uniformity of the cell spheroid microenvironment.

[0017] Furthermore, this application achieves purely mechanical control of the gas proportioning device, eliminating the need for electronic sensors and solenoid valve systems, effectively simplifying the equipment structure. The overall structure can operate without a power supply, which not only reduces equipment manufacturing costs but also reduces maintenance requirements. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of a carbon dioxide proportioning device.

[0019] Figure 2 This is a three-dimensional diagram of a carbon dioxide mixing device.

[0020] Figure 3 This is a schematic diagram of the rotating crank in a carbon dioxide proportioning device.

[0021] Figure 4 A schematic diagram of the drive shaft in a carbon dioxide proportioning device. Figure 1 .

[0022] Figure 5 A schematic diagram of the drive shaft in a carbon dioxide proportioning device. Figure 2 .

[0023] Figure 6 This is a schematic diagram of the connecting rod in a carbon dioxide proportioning device.

[0024] Figure 7 This is a schematic diagram of the proportioning cylinder in a carbon dioxide proportioning device. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] In existing technologies, the spheroidization and culture of mesenchymal stem cells requires a quantitative concentration of carbon dioxide, and traditional methods rely on a gas supply mode that mixes high-pressure carbon dioxide cylinders with air. Such systems employ a control method that combines sensor monitoring with solenoid valve linkage, resulting in complex structures and high equipment costs. Especially in laboratory environments or medical equipment applications, frequent sensor calibration and maintenance, along with high solenoid valve failure rates, increase operating costs and affect equipment operational stability.

[0027] To address these issues, researchers discovered that traditional electronic control methods suffer from high system complexity. Inspired by the synchronicity of mechanical transmission, they proposed constructing a parallel gas input channel. By adjusting the amount of gas input per cycle, the sensor feedback mechanism can be replaced, thus forming a mechanical mixing scheme.

[0028] Please see Figure 1-7 This application proposes a carbon dioxide proportioning device, including two parallel gas input mechanisms. The output ends of the two gas input mechanisms are connected to the same gas storage tank 6. The input end of one gas input mechanism is connected to a gas source that stores only carbon dioxide, while the input end of the other gas input mechanism is placed in the air. The two gas input mechanisms are driven by the same drive device to synchronously and quantitatively input air and carbon dioxide into the gas storage tank 6 multiple times. The amount of air and carbon dioxide input in each instance is adjustable.

[0029] The gas input mechanism includes a fixed mixing cylinder 4 and a piston 41 disposed inside the mixing cylinder 4 and movable along the axial direction of the mixing cylinder 4. The mixing cylinder 4 can be fixed by a special clamp, the specific design of which will not be described in detail in this application. The piston 41 is driven by a power mechanism to reciprocate in the mixing cylinder 4. A three-way pipe 5 is fixed at the opening of the mixing cylinder 4. One-way valves are fixed on both ends of the three-way pipe 5. The one-way valves make one end of the three-way pipe 5 the input end and the other end the output end. As the piston 41 reciprocates in the mixing cylinder 4, gas is drawn and sent into the gas storage tank 6.

[0030] The proportioning cylinder 4 refers to a cylindrical cavity with a fixed volume, which can be made of metal or polymer materials. It is used to form a sealed space for gas suction and discharge, and its axial dimension determines the amount of gas delivered in a single operation. The piston uses a rubber sealing ring to achieve airtightness, and changes the effective volume inside the proportioning cylinder by axial displacement.

[0031] Specifically, the two mixing cylinders 4 are respectively connected to pure carbon dioxide and air. The piston 41 is driven to reciprocate synchronously by the power mechanism. The single delivery volume is changed by adjusting the piston stroke. After multiple deliveries, a predetermined proportion of mixed gas is formed in the gas storage tank 6. The mechanical linkage ensures that the number of air and carbon dioxide deliveries is strictly consistent.

[0032] Compared with existing technologies, this invention achieves simultaneous, multiple, quantitative input of air and carbon dioxide, precisely matching the carbon dioxide requirements for mesenchymal stem cell spheroidization culture. While simplifying the gas path structure and reducing equipment costs, it significantly improves gas mixing efficiency and stability, ensuring the uniformity of the cell spheroid microenvironment. This solution employs a purely mechanical structure to enhance operational reliability.

[0033] It should be noted that the mixing device of this application can be used not only for mixing carbon dioxide and air, but also for mixing any two gases. Furthermore, the gas input mechanism in this mixing device can be configured with multiple sets according to actual conditions, and the mixing ratio of various gases can all be achieved using this mixing device.

[0034] The power mechanisms of the two gas input mechanisms are connected in series, with one power mechanism being driven by a drive motor to rotate, thereby causing the two power mechanisms to rotate synchronously.

[0035] Specifically, the power mechanism includes a U-shaped rotating crank 1, a connecting rod 3, and a piston rod 42. Each of the two feet of the rotating crank 1 is equipped with an adjustable transmission shaft 2. The axis of the transmission shaft 2 is perpendicular to the axis of the foot of the rotating crank 1, and the fixed end of the transmission shaft 2 is circularly connected to the foot of the rotating crank 1, while the movable end rotates around the circumference of the fixed end. The rotating cranks 1 are connected in series via the transmission shaft 2 and rotate synchronously. The rotating crank 1 rotates around the axis of the transmission shaft 2. The middle part of the rotating crank 1 is rotatably connected to one end of the connecting rod 3, and the other end of the connecting rod 3 is rotatably connected to one end of the piston rod 42. One end of the piston rod 42 is also fixedly connected to the piston 41. When the rotating crank 1 rotates, it sequentially drives the piston 41 in the proportioning cylinder 4 to reciprocate through the connecting rod 3 and the piston rod 42.

[0036] To prevent piston 41 from falling out of proportioning cylinder 4, a limit plug 43 is threaded to the tail end of proportioning cylinder 4. Piston column 42 passes through limit plug 43 and is slidably connected to it. A connecting ring 44 is fixedly connected to the end of piston column 42 away from piston 41. The connecting ring 44 is connected to the end of connecting rod 3 by a pin that passes through both, so as to realize the rotational connection between connecting rod 3 and piston column 42.

[0037] Multiple vent holes are provided on the limiting plug 43 to ensure that no high pressure resistance is formed between the piston 41 and the limiting plug 43 inside the proportioning cylinder 41 when the piston 41 is running.

[0038] Furthermore, all the drive shafts 2 are coaxially arranged, and the two drive shafts 2 that are furthest apart are rotatably connected to a bearing seat. One end of the two drive shafts 2 is connected to the drive shaft of an external drive motor. The movable end of the drive shaft 2 between the two rotating cranks 1 abuts and is fixed. The drive motor drives all the rotating cranks 1 to rotate through the drive shaft 2.

[0039] In the absence of power, a hand-cranked turntable or handle can be installed at the end of the drive shaft 2 to drive the crank 1 to rotate, and the proportioning work can still be completed.

[0040] Furthermore, both feet of the crank 1 are provided with external threads 12, and the fixed end of the transmission shaft 2 has a fixed cylinder 23 fixedly connected to the transmission shaft 2. A threaded cylinder 24 is rotatably connected inside the fixed cylinder 23. The threaded cylinder 24 has an internal thread that matches the external threads 12 on the feet of the crank 1. By rotating the threaded cylinder 24, the position of the threaded cylinder 24 on the feet of the crank 1 can be adjusted.

[0041] The movable end of the drive shaft 2 is fixed with a detachable connecting flange 22, and the connecting flanges 22 are fixed together by bolts to realize the connection between the drive shafts 2.

[0042] It should be noted that the connection structure between the connecting flange 22 and the drive shaft 2 can be bolted. The bolt passes through the connecting flange 22 along the axial direction of the drive shaft 2 and is fixed to the end of the drive shaft 2. In order to ensure that the connecting flange 22 does not interfere when it is in contact, a recessed groove can be opened on the side of the connecting flange away from the drive shaft 2, or countersunk bolts can be used.

[0043] The fixed cylinder 23 is equipped with a set screw that fixes the threaded cylinder 24 and the fixed cylinder 23. By tightening the set screw, the fixed cylinder 23 and the threaded cylinder 24 can rotate synchronously. At this time, the threaded cylinder 24 can be rotated through the transmission shaft 2, thereby adjusting its position on the crank 1 foot.

[0044] The two feet of the crank 1 are also provided with grooves 13 extending along the axis of the feet. The grooves 13 are provided with scale lines, which can be used as a reference when adjusting the position of the threaded cylinder 24.

[0045] The connecting rod 3 is connected to one end of the rotating crank 1 and is provided with a connecting ring 31. The inner diameter of the connecting ring 31 is the same as the shaft diameter in the middle of the rotating crank 1. The connecting ring 31 includes two opposing semicircular rings. One semicircular ring is fixedly connected to the connecting rod 3. One end of the other semicircular ring is hinged to one end of the aforementioned semicircular ring through a hinge. The other end is fastened together by a J-shaped or U-shaped connector. The two legs of the connector pass through the two semicircular rings in a direction parallel to the axis of the connecting ring 31.

[0046] To secure the connector, at least one of the feet is threaded with a nut or a pin after passing through the semicircular ring to fix the connector.

[0047] Through the above technical solution, this application achieves purely mechanical control of the gas proportioning device, eliminating the need for electronic sensors and solenoid valve systems, effectively simplifying the equipment structure. The fixed-volume proportioning cylinder and piston linkage design ensures that the gas delivery rate depends solely on mechanical motion parameters, reducing reliance on precision electronic components. The combination of a one-way valve and a three-way pipe prevents gas backflow, ensuring the stability of unidirectional gas flow. The overall structure can operate without a power supply, reducing both equipment manufacturing costs and maintenance requirements.

[0048] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention. Furthermore, the contents not described in detail in this specification are all prior art known to those skilled in the art.

Claims

1. A carbon dioxide proportioning device, characterized by comprising: It includes two parallel gas input mechanisms, the output ends of which are connected to the same gas storage tank (6). The input end of one gas input mechanism is connected to a gas source that stores only carbon dioxide, while the input end of the other gas input mechanism is placed in the air. The two gas input mechanisms are driven by the same drive device, and synchronously and quantitatively input air and carbon dioxide into the gas storage tank (6) multiple times. The amount of air and carbon dioxide input in a single input is adjustable.

2. The carbon dioxide proportioner of claim 1, wherein, The gas input mechanism includes a fixed mixing cylinder (4) and a piston (41) installed inside the mixing cylinder (4) that can move along the axial direction of the mixing cylinder (4). The piston (41) is driven by a power mechanism to reciprocate in the mixing cylinder (4). A three-way pipe (5) is fixed at the opening of the mixing cylinder (4). A one-way valve is fixed on both sides of the three-way pipe (5). The one-way valve makes one side of the three-way pipe (5) form the input end and the other side form the output end. As the piston (41) reciprocates in the mixing cylinder (4), the gas is drawn and sent into the gas storage tank (6).

3. The carbon dioxide proportioner of claim 2, wherein, The power mechanisms of the two gas input mechanisms are connected in series, with one power mechanism being driven by a drive motor to rotate, thereby causing the two power mechanisms to rotate synchronously.

4. The carbon dioxide proportioning device according to claim 2 or 3, characterized in that, The power mechanism includes a U-shaped rotating crank (1), a connecting rod (3), and a piston rod (42). Each of the two feet of the rotating crank (1) is equipped with an adjustable transmission shaft (2). The axis of the transmission shaft (2) is perpendicular to the axis of the foot of the rotating crank (1), and the fixed end of the transmission shaft (2) is connected to the foot of the rotating crank (1), while the movable end rotates around the fixed end. The rotating cranks (1) are connected in series through the transmission shaft (2) and rotate synchronously. The rotating crank (1) rotates around the axis of the transmission shaft (2). The middle part of the rotating crank (1) is rotatably connected to one end of the connecting rod (3), and the other end of the connecting rod (3) is rotatably connected to one end of the piston rod (42). One end of the piston rod (42) is fixedly connected to the piston (41). When the rotating crank (1) rotates, the piston (41) in the proportioning cylinder (4) is driven to reciprocate through the connecting rod (3) and the piston rod (42) in sequence.

5. The carbon dioxide proportioner of claim 4, wherein, All the drive shafts (2) are coaxially arranged. The two drive shafts (2) that are furthest apart are rotatably connected to a bearing seat. One end of the two drive shafts (2) is connected to the drive shaft of a fixedly installed drive motor. The movable end of the drive shaft (2) between the two rotating cranks (1) abuts and is fixed. The drive motor drives all the rotating cranks (1) to rotate through the drive shaft (2).

6. The carbon dioxide proportioner of claim 4, wherein, Both feet of the rotating crank (1) are provided with external threads (12). The fixed end of the transmission shaft (2) has a fixed cylinder (23) fixedly connected to the transmission shaft (2). A threaded cylinder (24) is rotatably connected inside the fixed cylinder (23). The threaded cylinder (24) has an internal thread that matches the external threads (12) on the feet of the rotating crank (1). By rotating the threaded cylinder (24), the position of the threaded cylinder (24) on the feet of the rotating crank (1) can be adjusted.

7. The carbon dioxide proportioning device according to claim 5 or 6, characterized in that The movable end of the drive shaft (2) is fixed with a detachable connecting flange (22), and the connecting flanges (22) are fixed together by bolts to realize the connection between the drive shafts (2).

8. The carbon dioxide proportioner of claim 6, wherein, The fixed cylinder (23) is provided with a set screw for fixing the threaded cylinder (24) and the fixed cylinder (23). By tightening the set screw, the fixed cylinder (23) and the threaded cylinder (24) can rotate synchronously. At this time, the threaded cylinder (24) can be rotated through the transmission shaft (2), thereby adjusting its position on the crank (1) foot.

9. The carbon dioxide proportioner of claim 6, wherein, The two feet of the rotating crank (1) are also provided with grooves (13) extending along the axis of the feet. The grooves (13) are provided with scale lines, which can be used as a reference when adjusting the position of the threaded cylinder (24).

10. The carbon dioxide proportioner of claim 4, wherein, The connecting rod (3) is connected to the crank (1) at one end with a connecting ring (31). The inner diameter of the connecting ring (31) is the same as the shaft diameter at the middle of the crank (1). The connecting ring (31) includes two opposing semicircular rings. One semicircular ring is fixedly connected to the connecting rod (3). One end of the other semicircular ring is hinged to one end of the aforementioned semicircular ring through a hinge. The other end is fastened together through a J-shaped or U-shaped connector. The two legs of the connector pass through the two semicircular rings in a direction parallel to the axis of the connecting ring (31).

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