Petal-shaped tension chip and pouring mold thereof
By designing a petal-shaped tension chip and its casting mold, the problem of the difficulty in multi-group cell culture and experimentation in existing organ-on-a-chip has been solved. It realizes diversified mechanical stimulation simulation and drug stimulation combination, improves the physiological simulation accuracy and experimental flexibility of organ-on-a-chip, and is suitable for cardiovascular, respiratory system and tissue engineering research.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- JINLIN MEDICAL COLLEGE
- Filing Date
- 2025-05-16
- Publication Date
- 2026-04-21
AI Technical Summary
Existing organ-on-a-chip systems are difficult to conduct multiple cell cultures and experiments simultaneously, and their mechanical stimulation simulation is not flexible enough to meet the diverse needs of cell culture and research.
A petal-shaped tension chip and its casting mold were designed, comprising a petal-shaped culture structure, a cell culture chamber, an immersion channel, a liquid exchange channel, a pressure tank, and a gas channel. The cell attachment membrane treated with plasma oxygen is bonded to the petal-shaped culture structure to simulate a diverse mechanical microenvironment, and the cell morphology is affected by the tension applied by the pressure tank.
It enables flexible adjustment of multiple cell cultures and experiments, simulates diverse combinations of mechanical and drug stimuli, and improves the physiological simulation accuracy and experimental flexibility of organ-on-a-chip, making it suitable for fields such as cardiovascular, respiratory system, and tissue engineering.
Smart Images
Figure CN224148074U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of biotechnology, and specifically relates to a petal-shaped tension chip and its casting mold. Background Technology
[0002] Organ-on-a-chip (OAS) is a biotechnology that simulates and reproduces the microenvironments of various living cells, tissues, and organs within microfluidic chips; this technology can reproduce the mechanisms of action in vivo in vitro. Different types of OAS, such as intestinal-on-a-chip, heart-on-a-chip, lung-on-a-chip, liver-on-a-chip, brain-on-a-chip, and tumor-on-a-chip, have been developed and are widely used in drug screening, disease simulation, personalized medicine, and toxicity testing. Among these OAS, simulating and reproducing the personalized biomechanical environment of cells is particularly important, such as simulating the periodic contraction and relaxation of alveoli, blood flow in blood vessels, the regular beating of the heart, the movement of fluid in bone pores, and the peripheral environment of tumor cell migration. While existing OAS can simulate certain mechanical stimuli, they cannot simultaneously conduct controlled experiments. Utility Model Content
[0003] The purpose of this invention is to provide a petal-shaped tension chip and its casting mold, which can meet diverse cell culture and research needs and simultaneously conduct multiple cell cultures and experiments.
[0004] The technical solution provided by this utility model is as follows:
[0005] A petal-shaped tension chip, comprising:
[0006] The petal-shaped culture structure has a cylindrical shape.
[0007] The petal-shaped culture structure includes:
[0008] Five cell culture structures are arranged in a petal-like pattern;
[0009] The cell culture structure includes:
[0010] Cell culture chamber, which is a cylindrical groove;
[0011] The immersion channel and the medium exchange channel are connected at one end to the cell culture chamber; the immersion channel and the medium exchange channel are symmetrically arranged about the center of the cell culture chamber.
[0012] Two air pressure grooves, each a semi-circular annular groove; the two air pressure grooves are symmetrically arranged about the center of the cell culture chamber;
[0013] A gas passage, one end of which is connected to the two pressure tanks respectively;
[0014] The immersion channel, the liquid exchange channel, and the gas channel are all arranged radially along the petal-shaped culture structure.
[0015] The cell attachment membrane, after being treated with plasma oxygen, bonds and seals itself to the petal-shaped culture structure.
[0016] Preferably, the cell culture structure further includes:
[0017] The immersion channel interface is a circular through hole, and the immersion channel interface is located at the other end of the immersion channel and communicates with the immersion channel.
[0018] The fluid exchange channel interface is a circular through hole, and the fluid exchange channel interface is located at the other end of the fluid exchange channel and communicates with the fluid exchange channel.
[0019] A gas channel interface, which is a circular through hole, is located at the other end of the gas channel and communicates with the gas channel.
[0020] Preferably, the cell attachment membrane is an elastic PDMS membrane.
[0021] Preferably, the thickness of the cell attachment membrane is 50-100 μm.
[0022] Preferably, the diameter of the void circle formed by the other end of the medium exchange channel of the five cell culture structures is 2.4 mm; the distance from the gas channel interface of the five cell culture structures to the edge of the petal-shaped culture structure is 2 mm.
[0023] Preferably, the distance between the outer wall of the cell culture chamber and the inner ring of the air pressure groove is 1.2 mm.
[0024] Preferably, the cell culture chamber has a diameter of 3 mm; the inner diameter of the pressure tank is 5.4 mm and the outer diameter is 7.8 mm; the width of the immersion channel and the liquid exchange channel is 0.4 mm; the width of the gas channel is 0.5 mm; and the diameters of the immersion channel interface, the liquid exchange channel interface, and the gas channel interface are all 1.2 mm.
[0025] Preferably, the height of the cell culture chamber is 4 mm; the height of the air pressure tank is 3 mm; and the heights of the immersion channel, the liquid exchange channel, and the gas channel are all 0.6 mm.
[0026] Preferably, the gas channel includes:
[0027] A first gas passage, one end of which is connected to the gas passage interface;
[0028] Two second gas channels, one end of which is connected to the other end of the first gas channel;
[0029] Two third gas channels, one end of which is connected to the pressure tank and the other end of which is connected to the other end of the second gas channel; the two third gas channels are arranged parallel to each other on both sides of the immersion channel;
[0030] The two second gas channels, the two third gas channels, and the two pressure grooves are connected in a one-to-one correspondence; the second gas channel has an angle with the first gas channel and the third gas channel.
[0031] Preferably, a casting mold for a petal-shaped tension chip is provided for casting the petal-shaped culture structure of the petal-shaped tension chip, the casting mold comprising:
[0032] The pouring height control frame is a circular ring;
[0033] The petal-shaped culture structure molding embossing structure has a cylindrical base that matches and fits into the casting height control frame; the upper surface of the petal-shaped culture structure molding embossing structure is provided with a embossed pattern corresponding to the petal-shaped culture structure;
[0034] The casting height control frame is located on the outside of the petal-shaped culture structure molding plate structure.
[0035] The beneficial effects of this utility model are:
[0036] The petal-shaped tension chip provided by this invention simulates diverse mechanical microenvironments, flexibly adjusts the combination of mechanical stimulation and drug stimulation, and simultaneously performs multiple cell cultures and experiments.
[0037] The petal-shaped tension chip casting mold provided by this utility model has a simple structure and can efficiently manufacture petal-shaped tension chips, thus meeting the needs of petal-shaped tension chip manufacturing. Attached Figure Description
[0038] Figure 1 This is a schematic diagram of the petal-shaped culture structure described in this utility model.
[0039] Figure 2 This is a schematic diagram of the cell culture structure described in this utility model.
[0040] Figure 3 This is a schematic diagram of the petal-shaped culture structure compression molding plate structure described in this utility model. Detailed Implementation
[0041] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.
[0042] like Figure 1 As shown, this utility model provides a petal-shaped tension chip and its casting mold, which includes:
[0043] The petal-shaped culture structure 100 is cylindrical in shape; the petal-shaped culture structure 100 includes five cell culture structures, which are evenly distributed in a petal shape.
[0044] The cell culture structure includes: a cell culture chamber 110, which is a cylindrical groove; an immersion channel 120 and a medium exchange channel 130, one end of which is connected to the cell culture chamber 110; the immersion channel 120 and the medium exchange channel 130 are symmetrically arranged about the center of the cell culture chamber 110; an immersion channel interface 121, which is a circular through hole, is located at the other end of the immersion channel 120 and is connected to the immersion channel 120; a medium exchange channel 131, which is a circular through hole, is located at the other end of the medium exchange channel 130 and is connected to the medium exchange channel 130; two pressure tanks 140, both of which are semi-circular annular grooves; the two pressure tanks 140 are symmetrically arranged about the center of the cell culture chamber 110; and a gas channel 150, one end of which is connected to each of the two pressure tanks 140; wherein the immersion channel 120, the medium exchange channel 130, and the gas channel 150 are... All are arranged radially along the petal-shaped culture structure 100; a gas channel interface 151 is a circular through hole, the gas channel interface 151 is located at the other end of the gas channel 150 and communicates with the gas channel 150; the gas channel 150 includes: a first gas channel 152, one end of which communicates with the gas channel interface 151; two second gas channels 153, one end of which communicates with the other end of the first gas channel 152; two third gas channels 154, one end of which communicates with the pressure tank 140 and the other end of which communicates with the other end of the second gas channel 153; the two third gas channels 154 are arranged parallel to each other on both sides of the immersion channel 120; wherein, the two second gas channels 153, the two third gas channels 154 and the two pressure tanks 140 are connected in a one-to-one correspondence; the second gas channel 153 has an included angle with the first gas channel 152 and the third gas channel 154.
[0045] The cell attachment membrane, after being treated with plasma oxygen, is bonded and sealed to the petal-shaped culture structure 100; the cell attachment membrane is an elastic PDMS membrane with a thickness of 50-100 μm; the cell attachment membrane has a certain stretching and recovery ability, and can still maintain a good working state after several stretchings; cells attach and grow on the cell attachment membrane, and can grow into a monolayer of cells under good conditions; the cell attachment membrane is a transparent membrane, which facilitates the observation of cell growth status and cell growth morphology under a microscope.
[0046] The cells to be observed are placed in the cell culture chamber 110 for culture, sample addition, reaction, and observation. A pulling force is applied through the air pressure tank 140, causing deformation of the cell adhesion membrane, which in turn affects the cell morphology, allowing observation of the impact of different cell morphologies on the reaction. Simultaneously, nutrients can be injected into the cell culture chamber 110 through the immersion channel 120, and the medium can be changed through the medium exchange channel 130, removing ineffective liquid, thus achieving continuous cell culture in the petal-shaped tension chip. The petal-shaped culture structure 100 is made of transparent material, facilitating observation of the petal-shaped tension chip's usage and cell morphology under a microscope.
[0047] In this embodiment, the diameter of the gap circle formed by the other end of the medium exchange channel 130 of the five cell culture structures is 2.4 mm; the distance from the gas channel interface 151 of the five cell culture structures to the edge of the petal-shaped culture structure 100 is 2 mm.
[0048] like Figure 2 As shown, in this embodiment, the distance between the outer wall of the cell culture chamber 110 and the inner ring of the air pressure groove 140 is 1.2 mm.
[0049] In this embodiment, the cell culture chamber 110 has a diameter of 3 mm and a height of 4 mm.
[0050] In this embodiment, the inner diameter of the air pressure groove 140 is 5.4 mm, the outer diameter is 7.8 mm, and the height is 3 mm.
[0051] In this embodiment, the width of both the immersion channel 120 and the liquid exchange channel 130 is 0.4 mm; the length of the immersion channel 120 is 3 mm; the length of the liquid exchange channel 130 is 4.6 mm; wherein, the liquid exchange channel 130 includes: a first liquid exchange channel 132 and a second liquid exchange channel 133, one end of the first liquid exchange channel 132 is connected to the cell culture chamber 110, one end of the second liquid exchange channel 133 is connected to the liquid exchange channel 131, and the other end of the first liquid exchange channel 132 and the other end of the second liquid exchange channel 133 are connected at an angle, the length of the first liquid exchange channel 132 is 3 mm, and the length of the second liquid exchange channel 133 is 1.6 mm.
[0052] In this embodiment, the width of the gas channel 150 is 0.5 mm; the angle θ between the two second gas channels 153 is 60°~80°; the length of the first gas channel 152 is 1.5 mm; the length of the third gas channel 154 is 3 mm; and the radial length of the second gas channel 153 is 3.3 mm.
[0053] In this embodiment, the heights of the immersion channel 120, the liquid exchange channel 130, and the gas channel 150 are all 0.6 mm.
[0054] In this embodiment, the diameters of the immersion channel interface 121, the liquid exchange channel 131, and the gas channel interface 151 are all 1.2 mm.
[0055] like Figure 3 As shown, a casting mold for a petal-shaped tension chip is used to cast a petal-shaped culture structure 100 of the petal-shaped tension chip. The casting mold includes: a casting height control frame, which is an annular ring; a petal-shaped culture structure pressing plate structure 200, the base of which is a cylinder 210 and is matched and fitted with the casting height control frame; the upper surface of the petal-shaped culture structure pressing plate structure 200 is provided with a raised pattern 220 corresponding to the petal-shaped culture structure 100; wherein, the casting height control frame is located on the outside of the petal-shaped culture structure pressing plate structure 200.
[0056] After the casting height control frame is installed in conjunction with the petal-shaped culture structure pressing plate structure 200 to form a casting mold, the gel is poured into the casting mold, and the corresponding spatial structure is formed by the plate pattern 220. After the gel solidifies, the gel is separated from the casting mold to obtain a petal-shaped tension gel chip with a petal-shaped culture structure 100, which is used for cell experiments.
[0057] The experimental procedure is as follows: Step 1, chip assembly: After immersing all glass items in sulfuric acid for 12 hours, remove them while wearing gloves, rinse off the remaining sulfuric acid with running water, rinse several times with 75% alcohol, then clean them in distilled water, and place them on a slide rack to dry for later use; clean silicone items with a special detergent; prepare the cell attachment membrane; assemble the petal-shaped culture structure 100 with the cell attachment membrane, and bond and seal the cell attachment membrane to the petal-shaped culture structure 100 after plasma oxygen treatment, so that the cell attachment membrane is tightly adsorbed on the petal-shaped culture structure 100 to ensure that no liquid seepage occurs.
[0058] The second step involves planting cells in the cell culture chamber 110, sealing it, and injecting the nutrient solution required for cell culture into the cell culture chamber 110 through the immersion channel interface 121 and the immersion channel 120. After completion, the petal-shaped tension chip is placed in a carbon dioxide constant temperature incubator for culture. After a period of time, it is removed for observation. If the cell culture meets the required standards, the third step is performed; if the culture medium is insufficient, negative pressure is applied through the medium replacement channel 131 and the medium replacement channel 130 to extract the original nutrients and other waste liquid, and the nutrient solution is re-injected into the cell culture chamber 110 through the immersion channel interface 121 and the immersion channel 120 for culture.
[0059] Step 3: Applying tension for testing: After cell culture is completed, the seal is removed, and the gas channel interface 151 is connected to negative pressure, so that gas passes through the gas channel 150 to control the pressure tank 140, causing the pressure tank 140 to deform, which in turn causes the cell attachment membrane to deform, applying tension to the cells, thereby observing the cell morphology and structure.
[0060] This invention provides a petal-shaped tension chip. The petal-shaped multi-petal layout simulates the geometry of natural organs (such as heart valves and alveolar clusters), enhancing the biomimicry of the cellular microenvironment and strengthening the physiological relevance of mechanical stimulation. Arc-shaped pressure grooves are distributed along the edges of the petals, allowing independent or collaborative control of the stretching amplitude of each petal, achieving precise simulation of complex mechanical microenvironments (such as shear force and periodic tension). The modular petal design includes five petals, each independently or collaboratively controllable, providing 360° stretching, fluid, or drug stimulation to adapt to diverse experimental needs (such as multi-organ combination and gradient drug testing). This petal-shaped tension chip can meet diverse cell culture and research needs, simulate diverse mechanical microenvironments, flexibly adjust the combination of mechanical and drug stimulation, and simultaneously conduct multiple cell cultures and experiments. It significantly improves the physiological simulation accuracy and experimental flexibility of organ-on-a-chip, exhibiting significant advantages in complex mechanical environment simulation, long-term cell culture stability, and high-throughput experimental compatibility. It is suitable for cutting-edge research fields such as cardiovascular, respiratory, and tissue engineering.
[0061] The petal-shaped tension chip casting mold provided by this utility model has a simple structure and can efficiently manufacture petal-shaped tension chips, thus meeting the needs of petal-shaped tension chip manufacturing.
[0062] Although the embodiments of this utility model have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for this utility model. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, this utility model is not limited to the specific details and the illustrations shown and described herein.
Claims
1. A petal-shaped tension chip, characterized by, include: The petal-shaped culture structure has a cylindrical shape. The petal-shaped culture structure includes: Five cell culture structures are arranged in a petal-like pattern; The cell culture structure includes: Cell culture chamber, which is a cylindrical groove; The immersion channel and the medium exchange channel are connected at one end to the cell culture chamber; the immersion channel and the medium exchange channel are symmetrically arranged about the center of the cell culture chamber. Two air pressure grooves, each a semi-circular annular groove; the two air pressure grooves are symmetrically arranged about the center of the cell culture chamber; A gas passage, one end of which is connected to the two pressure tanks respectively; The immersion channel, the liquid exchange channel, and the gas channel are all arranged radially along the petal-shaped culture structure. The cell attachment membrane, after being treated with plasma oxygen, bonds and seals itself to the petal-shaped culture structure.
2. The petal-shaped tension chip of claim 1, wherein, The cell culture structure also includes: The immersion channel interface is a circular through hole, and the immersion channel interface is located at the other end of the immersion channel and communicates with the immersion channel. The fluid exchange channel interface is a circular through hole, and the fluid exchange channel interface is located at the other end of the fluid exchange channel and communicates with the fluid exchange channel. A gas channel interface, which is a circular through hole, is located at the other end of the gas channel and communicates with the gas channel.
3. The petal tension core of claim 1, wherein, The cell attachment membrane is an elastic PDMS membrane.
4. The petal tension core of claim 3, wherein, The thickness of the cell attachment membrane is 50-100 μm.
5. The petal tension core of claim 2, wherein, The diameter of the void circle formed by the other end of the medium exchange channel of the five cell culture structures is 2.4 mm; the distance from the gas channel interface of the five cell culture structures to the edge of the petal-shaped culture structure is 2 mm.
6. The petal tension core of claim 5, wherein, The distance between the outer wall of the cell culture chamber and the inner ring of the air pressure groove is 1.2 mm.
7. The petal-shaped tension chip of claim 6, wherein, The cell culture chamber has a diameter of 3 mm; the inner diameter of the pressure tank is 5.4 mm, and the outer diameter is 7.8 mm; the width of the immersion channel and the liquid exchange channel is 0.4 mm; the width of the gas channel is 0.5 mm; and the diameters of the immersion channel interface, the liquid exchange channel interface, and the gas channel interface are all 1.2 mm.
8. The petal-shaped tension chip of claim 7, wherein, The cell culture chamber has a height of 4 mm; the air pressure tank has a height of 3 mm; and the immersion channel, the liquid exchange channel, and the gas channel all have a height of 0.6 mm.
9. The petal-shaped tension chip of claim 8, wherein, The gas channel includes: A first gas passage, one end of which is connected to the gas passage interface; Two second gas channels, one end of which is connected to the other end of the first gas channel; Two third gas channels, one end of which is connected to the pressure tank and the other end of which is connected to the other end of the second gas channel; the two third gas channels are arranged parallel to each other on both sides of the immersion channel; The two second gas channels, the two third gas channels, and the two pressure grooves are connected in a one-to-one correspondence; the second gas channel has an angle with the first gas channel and the third gas channel.
10. A casting mold for a petal-shaped tension core for casting a petal-shaped culture structure of a petal-shaped tension core as claimed in any one of claims 1 to 9, characterized in that, The casting mold includes: The pouring height control frame is a circular ring; The petal-shaped culture structure pressure mold relief structure has a cylindrical base and is matched and embedded with the pouring height control frame; the upper surface of the petal-shaped culture structure pressure mold relief structure is provided with a relief pattern corresponding to the petal-shaped culture structure. The pouring height control frame is arranged outside the petal-shaped culture structure pressure mold relief structure.