Rapid identification culture drug sensitivity kit for salmonella

Abstract

The utility model provides a kit for rapidly identifying and culturing drug sensitivity of salmonella, and relates to the field of salmonella culture. The salmonella rapid identification culture drug sensitivity kit comprises a kit body and a kit cover, a container used for culturing salmonella is fixedly installed in the kit body, a base plate is fixedly installed on the inner bottom wall of the container, a telescopic rod used for rotating is fixedly connected to the base plate, a filter screen and an arc-shaped plate are fixedly installed at the movable end of the telescopic rod, and the arc-shaped plate is fixedly connected with the filter screen. The filter screen is slidably connected with the container; the rapid identification and culture drug sensitivity kit for salmonella integrates sample separation, dynamic culture and drug sensitivity test, after culture is completed, a drug sensitivity reagent can be directly added for the drug sensitivity test, sample transfer is not needed, operation steps and time are reduced, rapid identification and drug sensitivity detection of salmonella are achieved, and the kit is suitable for large-scale popularization and application. And the detection efficiency and accuracy are improved.

Description

Technical Field

[0001] This utility model relates to the field of Salmonella culture, specifically a rapid identification and culture drug susceptibility kit for Salmonella. Background Technology

[0002] Salmonella identification is performed using microbiological methods to determine the presence of Salmonella in a sample. Samples containing Salmonella are inoculated onto a specific culture medium to provide a suitable growth environment for the bacteria, allowing them to multiply rapidly and obtain a sufficient number for identification, drug sensitivity testing, and other studies. Selective media, such as SS agar, are typically used to inhibit the growth of other bacteria, facilitating Salmonella isolation and culture. During culture, temperature, humidity, and gas conditions must be carefully controlled, usually at a constant temperature of 37°C for 18-24 hours to allow Salmonella to form visible colonies. Drug sensitivity testing determines the susceptibility of Salmonella to various antimicrobial agents to guide rational clinical drug use. Kits integrate all necessary reagents, consumables, and operating instructions, eliminating the need for users to prepare and configure reagents themselves. This simplifies the experimental process, allowing even less experienced operators to follow the instructions correctly, reducing the difficulty and complexity of the experiment.

[0003] Traditional test kits involve culturing Salmonella samples in the culture medium provided within the kit. During this process, nutrients diffuse naturally, leading to slow Salmonella growth and a testing cycle that can take 3-5 days, resulting in a prolonged testing time. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a rapid identification, culture, and drug susceptibility testing kit for Salmonella, which solves the problem of long detection times.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution: a rapid identification and culture drug susceptibility kit for Salmonella, comprising a box body and a box lid, wherein a container for culturing Salmonella is fixedly installed inside the box body, a base plate is fixedly installed on the inner bottom wall of the container, a telescopic rod for rotation is fixedly connected to the base plate, a filter screen and an arc plate are fixedly installed on the movable end of the telescopic rod, and the filter screen is slidably connected to the container;

[0006] The filter screen is used to separate the mixture of culture medium and sample into upper and lower layers. The arc plate is used to rotate in the upper mixture inside the container. An air jet pipe is fixedly installed in the chassis and is used to inject a mixture of oxygen and carbon dioxide into the bottom of the container.

[0007] Preferably, the telescopic rod includes a fixed rod and a sleeve, the fixed rod and the sleeve are slidably connected, one end of the sleeve passes through the lid and is slidably and rotatably connected to the lid.

[0008] Preferably, the outer surface of the fixing rod is provided with a sliding groove, a slider is fixedly connected inside the sleeve, the slider is slidably connected to the sliding groove, and the end of the sleeve away from the chassis is connected to an external power source.

[0009] Preferably, a partition is fixedly connected inside the box body, the upper surface of the partition is concave, the partition is fixed to the container, and a feed inlet is opened on the outer surface of the container.

[0010] Preferably, a baffle is fixedly connected to the outer surface of the fixing rod, and the baffle contacts the bottom of the sleeve.

[0011] Preferably, the chassis includes a turntable, a central cylinder, and several hollow shells, with the hollow shells communicating with the central cylinder and the jet pipes fixed to the hollow shells.

[0012] Preferably, an air supply pipe is fixedly installed on the outside of the box body, with the outlet end of the air supply pipe rotatably connected to the turntable and the inlet end connected to an external air pump.

[0013] Preferably, the upper surface of the box cover is connected to an exhaust pipe, and both the exhaust pipe and the jet pipe are equipped with one-way valves.

[0014] Compared with existing technologies, this invention has the following advantages: The kit integrates sample separation, dynamic culture, and drug susceptibility testing. After culture, drug susceptibility reagents can be added directly for testing without sample transfer, reducing operational steps and time. This enables rapid identification and drug susceptibility detection of Salmonella, improving detection efficiency and accuracy. The filter separates the sample and culture medium mixture into upper and lower layers. The upper layer uses dynamic culture, which reduces interference from impurities in the lower layer, ensuring a pure culture environment. Dynamic culture also allows Salmonella to grow better in the relatively pure upper culture medium, preventing impurities from affecting bacterial growth and subsequent detection. The lower layer mainly contains impurities intercepted by the filter. The delivery of mixed gas promotes gas flow throughout the container, preventing anaerobic or other unfavorable environments for Salmonella growth due to lack of gas exchange in the lower layer. Furthermore, gas flow can prevent impurity precipitation and accumulation to some extent, contributing to the stability of the entire culture system. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a sectional view of the front view of the box body of this utility model;

[0017] Figure 3 This is a sectional view of the front view of the box and container of this utility model;

[0018] Figure 4 This is a sectional view of the front view of the turntable of this utility model;

[0019] Figure 5 This is a sectional view of the front view of the telescopic rod of this utility model.

[0020] The components are: 1. Box body; 2. Box lid; 3. Container; 4. Chassis; 401. Turntable; 402. Central cylinder; 403. Hollow shell; 5. Telescopic rod; 501. Fixed rod; 502. Sleeve; 6. Filter screen; 7. Arc plate; 8. Jet pipe; 9. Slide groove; 10. Slider; 11. Exhaust pipe; 12. Partition plate; 13. Feed inlet; 14. Baffle plate; 15. Air supply pipe. Detailed Implementation

[0021] like Figures 1-5 As shown, a rapid identification and culture susceptibility test kit for Salmonella includes a box body 1 and a box lid 2. A container 3 for culturing Salmonella is fixedly installed inside the box body 1. A base plate 4 is fixedly installed on the inner bottom wall of the container 3. The base plate 4 includes a turntable 401, a central cylinder 402, and several hollow shells 403. The hollow shells 403 communicate with the central cylinder 402. An air jet pipe 8 is fixed to the hollow shells 403. An air supply pipe 15 is fixedly installed on the outer side of the box body 1. The outlet end of the air supply pipe 15 is rotatably connected to the turntable 401, and its inlet end is connected to an external air pump. A telescopic rod 5 for rotation is fixedly connected to the base plate 4. The telescopic rod 5 includes a fixed rod 501 and a sleeve 502. The fixed rod 501 and the sleeve 502 are slidably connected. One end of the sleeve 502 penetrates the box lid 2 and is connected to the box lid 2. The cover 2 is slidably and rotatably connected. The outer surface of the fixed rod 501 is provided with a groove 9. The sleeve 502 is fixedly connected with a slider 10. The slider 10 is slidably connected with the groove 9. The end of the sleeve 502 away from the chassis 4 is connected to an external power source. The external power source drives the sleeve 502 to rotate. The outer surface of the fixed rod 501 is fixedly connected with a baffle 14. The baffle 14 contacts the bottom of the sleeve 502. The baffle 14 can support the sleeve 502 and also limit the lowest position of the filter screen 6. The movable end of the telescopic rod 5 is fixedly installed with the filter screen 6 and the arc plate 7. The filter screen 6 is slidably connected to the container 3. The inside of the box 1 is fixedly connected with a partition 12. The upper surface of the partition 12 is concave. The partition 12 is fixed to the container 3. The outer surface of the container 3 is provided with a feed inlet 13.

[0022] The filter screen 6 is used to separate the mixture of culture medium and sample into upper and lower layers. The arc plate 7 is used to rotate in the upper mixture in the container 3. The base 4 is fixedly installed with a jet pipe 8. The inside of the jet pipe 8 is used to inject a mixture of oxygen and carbon dioxide into the bottom of the container 3. The upper surface of the lid 2 is connected to an exhaust pipe 11. Both the exhaust pipe 11 and the jet pipe 8 are equipped with one-way valves.

[0023] When using the container, first remove the lid 2 from the body 1, then pull the sleeve 502 upwards. The sleeve 502 will cause the filter screen 6 and several arc-shaped plates 7 to rise, making the filter screen 6 higher than the feed inlet 13. Then, mix the culture medium and the Salmonella sample and pour it onto the partition 12. The mixture of culture medium and sample can be in a liquid state. The upper surface of the partition 12 is concave. The mixture of culture medium and sample enters the container 3 through the feed inlet 13. The culture medium provides nutrients and suitable environmental conditions for the growth of Salmonella, while the sample contains microorganisms to be cultured. After mixing them, the microorganisms will grow and multiply in the culture medium.

[0024] Next, the sleeve 502 can be manually lowered to reset the filter screen 6 and the arc plate 7. It should be noted that when the sleeve 502 lowers the filter screen 6, the filter screen 6 moves from a high position to a low position inside the container 3. During the descent, larger particulate impurities in the mixture, such as tissue fragments and food residues, will be intercepted by the filter screen 6. As the filter screen 6 continues to descend, the larger particulate impurities gradually accumulate below the filter screen 6, while the culture medium and smaller Salmonella cells enter the area above the filter screen 6 through the pores of the filter screen 6, thereby separating the mixture into upper and lower layers. At the same time, during the descent of the filter screen 6, it slides in contact with the inner wall of the container 3, ensuring the sealing of the stratification process and avoiding unnecessary mixing of the upper and lower layers.

[0025] Next, after the filter screen 6 and the arc-shaped plate 7 are reset, the lid 2 is installed on the box body 1. Then, an external power source is connected to one end of the sleeve 502. The external power source can be a motor, used to drive the sleeve 502 to rotate. The sleeve 502 drives the fixed rod 501 to rotate through the cooperation of the slider 10 and the groove 9, thereby causing the arc-shaped plate 7 to rotate in the upper layer of the mixture in the container 3. The rotation of the arc-shaped plate 7 agitates the upper layer of culture medium and Salmonella mixture, allowing Salmonella to fully contact the culture medium, increasing the opportunity for the bacteria to obtain nutrients, and promoting the diffusion of metabolites, thus improving the culture efficiency. The jet pipe 8 in the chassis 4 is connected to an external air pump through the gas supply pipe 15. The air pump delivers a mixture of oxygen and carbon dioxide gas to the jet pipe 8 through the gas supply pipe 15. The jet pipe 8 injects the mixed gas into the bottom of the container 3. As the gas rises, it passes through the lower impurity layer, supplementing the upper culture environment with oxygen and promoting the aerobic respiration of Salmonella. At the same time, carbon dioxide can adjust the pH value of the culture medium. This keeps the mixture within a suitable range for Salmonella growth. Since both the exhaust pipe 11 and the jet pipe 8 are equipped with one-way valves, the exhaust pipe 11 only allows excess gas from container 3 to escape, preventing backflow of outside air and contamination of the culture environment. The jet pipe 8 ensures that gas can only be injected into container 3 in one direction, maintaining the stability of the culture environment. It should be noted that after the filter 6 separates the mixture into upper and lower layers, the upper layer mainly consists of culture medium and Salmonella. Dynamic culture reduces interference from impurities in the lower layer, ensuring a pure culture environment. Simultaneously, dynamic culture allows Salmonella to grow better in the relatively pure upper culture medium, avoiding the impact of impurities on bacterial growth and subsequent detection. The lower layer mainly consists of impurities intercepted by the filter 6. Transporting mixed gas promotes gas flow throughout container 3, preventing the lower layer from becoming anaerobic or forming other environments unfavorable to Salmonella growth due to lack of gas exchange. Furthermore, gas flow can, to some extent, prevent impurities from precipitating and accumulating, which is beneficial for maintaining the stability of the entire culture system.

[0026] Finally, after the culture is completed, antimicrobial susceptibility reagents can be added through the feed inlet 13 as needed. The continuous rotation of the arc plate 7 ensures that the antimicrobial susceptibility reagents come into full contact with Salmonella, facilitating the observation and detection of Salmonella's sensitivity to different drugs, thereby achieving rapid identification and antimicrobial susceptibility testing of Salmonella. It should be noted that this kit integrates sample isolation, dynamic culture, and antimicrobial susceptibility testing. After the culture is completed, antimicrobial susceptibility reagents can be added directly for antimicrobial susceptibility testing without the need to transfer samples, reducing operation steps and time, achieving rapid identification and antimicrobial susceptibility detection of Salmonella, and improving detection efficiency and accuracy.

[0027] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A rapid identification and culture antimicrobial susceptibility test kit for Salmonella, comprising a box body (1) and a box lid (2), characterized in that: The box (1) is fixedly installed with a container (3) for culturing Salmonella. A base plate (4) is fixedly installed on the inner bottom wall of the container (3). A telescopic rod (5) for rotation is fixedly connected to the base plate (4). A filter screen (6) and an arc plate (7) are fixedly installed on the movable end of the telescopic rod (5). The filter screen (6) is slidably connected to the container (3). The filter (6) is used to separate the mixture of culture medium and sample into upper and lower layers. The arc plate (7) is used to rotate in the upper mixture in the container (3). The jet pipe (8) is fixedly installed in the chassis (4). The jet pipe (8) is used to inject a mixture of oxygen and carbon dioxide into the bottom of the container (3).

2. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 1, characterized in that: The telescopic rod (5) includes a fixed rod (501) and a sleeve (502). The fixed rod (501) is slidably connected to the sleeve (502). One end of the sleeve (502) passes through the lid (2) and is slidably and rotatably connected to the lid (2).

3. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 2, characterized in that: The outer surface of the fixed rod (501) is provided with a sliding groove (9), and a slider (10) is fixedly connected inside the sleeve (502). The slider (10) is slidably connected to the sliding groove (9), and the end of the sleeve (502) away from the chassis (4) is connected to an external power source.

4. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 1, characterized in that: The box (1) is fixedly connected to a partition (12), the upper surface of the partition (12) is concave, the partition (12) is fixed to the container (3), and the outer surface of the container (3) is provided with a feed inlet (13).

5. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 2, characterized in that: A baffle (14) is fixedly connected to the outer surface of the fixing rod (501), and the baffle (14) contacts the bottom of the sleeve (502).

6. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 1, characterized in that: The chassis (4) includes a turntable (401), a central cylinder (402) and several hollow shells (403). The hollow shells (403) are connected to the central cylinder (402), and the jet pipe (8) is fixed to the hollow shells (403).

7. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 6, characterized in that: An air supply pipe (15) is fixedly installed on the outside of the box (1). The outlet end of the air supply pipe (15) is rotatably connected to the turntable (401), and its inlet end is connected to an external air pump.

8. The Salmonella rapid identification, culture, and antimicrobial susceptibility testing kit according to claim 1, characterized in that: The upper surface of the box cover (2) is connected to an exhaust pipe (11), and a one-way valve is installed in both the exhaust pipe (11) and the jet pipe (8).

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