Magnetic stirring and oxygen content linkage control system for suspension cell culture

Abstract

The utility model discloses a magnetic stirring and oxygen content linkage control system for suspension cell culture, which comprises a machine body, a placing table is arranged on the surface of the machine body, a culture tank is arranged on the upper portion of the placing table, a sealing cover is arranged on the upper portion of the culture tank, and a connector is arranged on the upper portion of the sealing cover. A mounting groove is formed in the machine body, a driving motor is arranged in the mounting groove, and a driving plate is arranged at the power output end of the driving motor. The limiting groove is formed in the bottom of the placement table, the driving plate is located in the limiting groove, the magnetic stirrer is arranged in the culture tank, the center magnetic block and the multiple sets of magnetic strips are arranged on the surface of the driving plate, and the driving motor drives the driving plate to rotate; the central magnetic force and the plurality of groups of magnetic strips drive the magnetic stirrer to rotate through the magnetic force, so that the magnetic stirrer rotates in the cell culture fluid, the suspension liquid is stirred, and the damage to cells is reduced compared with the traditional stirring mode.

Description

Technical Field

[0001] This invention relates to the field of suspended cell culture technology, and more specifically to a magnetic stirring and oxygen content linkage control system for suspended cell culture. Background Technology

[0002] Suspension culture refers to a tissue culture system in which single cells and small cell clusters are cultured in a liquid culture medium that is constantly agitated or shaken. It is a culture method for non-adherent cells. Some adherent cells can also be cultured using this method after adaptation and selection. Increasing the scale of suspension culture is relatively simple; just increase the volume. For depths exceeding 5 mm, the culture medium needs to be agitated; for depths exceeding 10 cm, CO2 and air need to be introduced into the deeper layers to ensure sufficient gas exchange. It is a culture method that uses shaking or rotating devices to keep cells constantly dispersed and suspended in the culture medium.

[0003] Current suspension cell culture processes require stirring and oxygen supply to the culture vessel. Traditional stirring methods can easily damage cells and make it impossible to monitor internal oxygen levels. Therefore, a new technical solution is needed to address this issue. Utility Model Content

[0004] The purpose of this invention is to provide a magnetic stirring and oxygen content linkage control system for suspension cell culture, which solves the problem that existing suspension cell culture processes require stirring and oxygen supply in the culture tank, and that traditional stirring methods can easily damage cells and cannot monitor the internal oxygen content.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a magnetic stirring and oxygen content linkage control system for suspension cell culture, comprising: a body, a placement platform on the surface of the body and a culture tank on the upper part of the placement platform, a sealing cover on the upper part of the culture tank and a connector on the upper part of the sealing cover, an installation groove inside the body and a drive motor inside the installation groove, a drive plate at the power output end of the drive motor, a limit groove at the bottom of the placement platform and the drive plate inside the limit groove, a magnetic stir bar inside the culture tank and a central magnetic block and several sets of magnetic strips on the surface of the drive plate, an oxygen supply mechanism on the side of the body and a delivery pipe on the upper part of the oxygen supply mechanism, the delivery pipe being connected to the connector, the connector extending to the lower part of the sealing cover and having an air outlet at its bottom, a concentration sensor and several sets of through-holes inside the sealing cover, and a control panel on the surface of the body and the control panel being connected to the power mechanism via wires.

[0006] In a preferred embodiment of this utility model, a fixing plate is provided on both sides of the machine body, and a support rod is provided on the upper part of the fixing plate. A lifting block is provided on the surface of the support rod, and a connecting block is provided on the side of the lifting block. An adjusting rod is provided inside the connecting block, and a bracket is provided at one end of the adjusting rod. A temperature sensor is provided inside the bracket, and the temperature sensor is electrically connected to the control panel through a wire.

[0007] In a preferred embodiment of this utility model, the lifting block, adjusting rod, and temperature sensor all have a certain amount of damping friction with the support rod, connecting block, and bracket.

[0008] In a preferred embodiment of the present invention, the magnetic stir bar is arranged in a cross shape with both sides slightly protruding from the surface.

[0009] In a preferred embodiment of this invention, the temperature sensor is inserted into one of a set of vents and extends into the interior of the culture tank.

[0010] In a preferred embodiment of the present invention, an electric heater is provided on the side of the placement platform and an electric heating wire is provided on the side of the motor heater. The electric heating wire extends into the interior of the placement platform and is distributed in a serpentine structure.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0012] This invention features a placement platform on the surface of the machine body, with a culture tank on top of the platform. A sealing cap is located on top of the culture tank, and a connector is positioned above the sealing cap. Inside the machine body, a drive motor is installed, with a drive plate at its power output end. A limiting groove is located at the bottom of the placement platform, and the drive plate is situated within the limiting groove. Inside the culture tank, a magnetic stir bar is installed, and the surface of the drive plate is equipped with a central magnetic block and several sets of magnetic strips. The drive motor rotates the drive plate, causing the central magnetic block and the sets of magnetic strips to magnetically drive the magnetic stir bar to rotate, thus influencing the cell... The culture medium is rotated to agitate the suspension, which reduces cell damage compared to traditional agitation methods. An oxygen supply mechanism is located on the side of the unit, and a delivery pipe is located on the upper part of the oxygen supply mechanism. The delivery pipe is connected to a connector, which extends to the lower part of the sealing cap and has an air outlet at its bottom. Inside the sealing cap, there is a concentration sensor and several sets of through-holes. The oxygen supply device delivers oxygen to the connector through the delivery pipe and discharges it through the air outlet. The concentration sensor monitors the oxygen content inside the culture tank and replenishes oxygen in time when it is insufficient. The air outlets facilitate air exchange. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a front view structural diagram of the present invention;

[0015] Figure 3 This is a schematic cross-sectional view of the present invention.

[0016] Figure 4 This is a schematic diagram of the internal structure of the placement platform of this utility model.

[0017] In the diagram: 1. Main body; 2. Control panel; 3. Oxygen supply mechanism; 4. Placement platform; 5. Fixing plate; 6. Support rod; 7. Lifting block; 8. Connecting block; 9. Adjusting rod; 10. Temperature sensor; 11. Incubator; 12. Sealing cap; 13. Connector; 14. Electric heater; 15. Delivery pipe; 16. Bracket; 17. Mounting slot; 18. Drive motor; 19. Drive plate; 20. Gas outlet; 21. Concentration sensor; 22. Air vent; 23. Electric heating wire; 24. Mounting slot; 25. Magnetic strip; 26. Central magnetic block; 27. Magnetic stir bar. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figure 1-4This utility model provides a technical solution: a magnetic stirring and oxygen content linkage control system for suspension cell culture, comprising: a body 1, a placement platform 4 on the surface of the body 1, a culture tank 11 on the upper part of the placement platform 4, a sealing cover 12 on the upper part of the culture tank 11, a connector 13 on the upper part of the sealing cover 12, an installation groove 17 inside the body 1, a drive motor 18 inside the installation groove 17, a drive plate 19 at the power output end of the drive motor 18, a limiting groove 24 at the bottom of the placement platform 4, and the drive plate 19 located inside the limiting groove 24; and a system for controlling the magnetic stirring and oxygen content linkage control system for suspension cell culture. A magnetic stir bar 27 is provided, and a central magnetic block 26 and several sets of magnetic strips 25 are provided on the surface of the drive plate 19. An oxygen supply mechanism 3 is provided on the side of the body 1, and a delivery pipe 15 is provided on the upper part of the oxygen supply mechanism 3. The delivery pipe 15 is connected to a connector 13. The connector 13 extends to the lower part of the sealing cover 12 and has an air outlet 20 at its bottom. A concentration sensor 21 and several sets of through air holes 22 are provided inside the sealing cover 12. A control panel 2 is provided on the surface of the body 1 and is connected to the power mechanism through wires. A placement platform 4 is provided on the surface of the body 1, and a culture tank is provided on the upper part of the placement platform 4. 11. A sealing cover 12 is provided on the upper part of the culture tank 11, and a connector 13 is provided on the upper part of the sealing cover 12. A drive motor 18 is provided inside the body 1, and a drive plate 19 is provided at the power output end of the drive motor 18. A limiting groove 24 is provided at the bottom of the placement platform 4, and the drive plate 19 is located inside the limiting groove 24. A magnetic stir bar 27 is provided inside the culture tank 11, and a central magnetic block 26 and several sets of magnetic strips 25 are provided on the surface of the drive plate 19. The drive plate 19 is rotated by the drive motor 18, so that the central magnetic block and several sets of magnetic strips 25 drive the magnetic stir bar 27 to rotate through magnetic force, so that it rotates in the cell culture medium, thereby achieving the rotation of the magnetic stir bar 27. Compared to traditional stirring methods, the stirring of the suspension reduces cell damage. An oxygen supply mechanism 3 is provided on the side of the body 1, and a delivery pipe 15 is provided on the upper part of the oxygen supply mechanism 3. The delivery pipe 15 is connected to the connector 13. The connector 13 extends to the lower part of the sealing cover 12 and has an air outlet 20 at its bottom. Inside the sealing cover 12, a concentration sensor 21 and several sets of through vents 22 are provided. The oxygen supply device delivers oxygen to the connector 13 through the delivery pipe 15 and discharges it through the air outlet 20. The concentration sensor 21 monitors the oxygen content inside the culture tank 11 and replenishes oxygen in time when it is insufficient. It also facilitates air exchange with the air outlets 22.

[0020] Further improvements, such as Figure 1As shown: Both sides of the body 1 are equipped with fixing plates 5, and the upper part of the fixing plates 5 is equipped with a support rod 6. The surface of the support rod 6 is equipped with a lifting block 7, and the side of the lifting block 7 is equipped with a connecting block 8. The connecting block 8 has a slidingly connected adjusting rod 9, and one end of the adjusting rod 9 is equipped with a bracket 16. The bracket 16 has a temperature sensor 10 installed inside, and the temperature sensor 10 is electrically connected to the control panel 2 via a wire. This setup allows for real-time monitoring of the temperature inside the culture tank 11. The temperature sensor 10 can accurately measure the temperature inside the culture tank 11 and display it on the control panel 2, facilitating timely adjustment of culture conditions by the operator to ensure cell growth at a suitable temperature.

[0021] Further improvements, such as Figure 1 As shown: There is a certain amount of damping friction between the lifting block 7, the adjusting rod 9 and the temperature sensor 10 and the support rod 6, the connecting block 8 and the bracket 16. The presence of damping friction can prevent the lifting block 7, the adjusting rod 9 and the temperature sensor 10 from moving unintentionally, thereby ensuring the stability and accuracy of the measurement results.

[0022] Further improvements, such as Figure 3 As shown: The magnetic stir bar 27 is arranged in a cross shape with slightly protruding sides. The magnetic stir bar 27 can more effectively stir the cell culture medium, making it more uniform. The cross shape also makes the stir bar more stable and less likely to deviate or rotate during the stirring process.

[0023] Further improvements, such as Figure 2 As shown: The temperature sensor 10 is inserted into one of the sets of air holes 22 and extends into the interior of the culture tank 11. By directly inserting the temperature sensor 10 into the air holes 22 inside the culture tank 11, the temperature inside the tank can be measured more accurately.

[0024] Further improvements, such as Figure 4 As shown: An electric heater 14 is provided on the side of the placement platform 4, and an electric heating wire 23 is provided on the side of the electric heater. The electric heating wire 23 extends into the interior of the placement platform 4 and is distributed in a serpentine structure. The combined design of the electric heater 14 and the electric heating wire 23 can quickly and evenly heat the placement platform 4 and the culture tank 11 on it. The serpentine structure of the electric heating wire 23 can increase the heating area and improve the heating efficiency. At the same time, this design also facilitates precise temperature control to ensure that the cells in the culture tank 11 grow at a suitable temperature.

[0025] Working Principle: This invention features a placement platform 4 on the surface of the body 1, with a culture tank 11 positioned above the platform 4. A sealing cover 12 is located above the culture tank 11, and a connector 13 is positioned above the sealing cover 12. A drive motor 18 is located inside the body 1, with a drive plate 19 at its power output end. A limiting groove 24 is located at the bottom of the placement platform 4, and the drive plate 19 is positioned inside the limiting groove 24. A magnetic stir bar 27 is located inside the culture tank 11, and a central magnetic block 26 and several sets of magnetic strips 25 are arranged on the surface of the drive plate 19. The drive motor 18 rotates the drive plate 19, causing the central magnetic block and the sets of magnetic strips 25 to magnetically drive the magnetic stir bar 27. 7. Rotation allows the cell culture medium to be stirred, reducing cell damage compared to traditional stirring methods. An oxygen supply mechanism 3 is provided on the side of the body 1, and a delivery pipe 15 is provided on the upper part of the oxygen supply mechanism 3. The delivery pipe 15 is connected to the connector 13. The connector 13 extends to the lower part of the sealing cover 12 and has an air outlet 20 at its bottom. Inside the sealing cover 12, there is a concentration sensor 21 and several sets of through vents 22. The oxygen supply device delivers oxygen to the connector 13 through the delivery pipe 15 and discharges it through the air outlet 20. The concentration sensor 21 monitors the oxygen content inside the culture tank 11 and replenishes oxygen in time when it is insufficient. It also works with the vents 22 to achieve air exchange.

[0026] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0027] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection", and "linkage" should be interpreted broadly, and can refer to mechanical or electrical connections, or internal connections between two components, or direct connections. "Up", "down", "left", "right", etc., are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may change.

[0028] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A magnetic stirring and oxygen content linkage control system for suspension cell culture, characterized in that: include: The machine body (1) has a placement platform (4) on its surface and a culture tank (11) on its upper part. The culture tank (11) has a sealing cover (12) on its upper part and a connector (13) on its upper part. The machine body (1) has an installation groove (17) inside and a drive motor (18) inside the installation groove (17). The drive motor (18) has a drive plate (19) at its power output end. The placement platform (4) has a limit groove (24) at its bottom and the drive plate (19) is located inside the limit groove (24). The culture tank (11) has a magnetic stirrer inside. The surface of the drive plate (19) is provided with a central magnetic block (26) and several sets of magnetic strips (25). The side of the body (1) is provided with an oxygen supply mechanism (3) and the upper part of the oxygen supply mechanism (3) is provided with a delivery pipe (15). The delivery pipe (15) is connected to the connector (13). The connector (13) extends to the lower part of the sealing cover (12) and has an air outlet (20) at its bottom. The interior of the sealing cover (12) is provided with a concentration sensor (21) and several sets of through air holes (22). The surface of the body (1) is provided with a control panel (2) and the control panel (2) is connected to the power mechanism through wires.

2. The magnetic stirring and oxygen content linkage control system for suspension cell culture according to claim 1, characterized in that: The body (1) is provided with a fixing plate (5) on both sides and a support rod (6) on the upper part of the fixing plate (5). The support rod (6) is provided with a lifting block (7) on its surface and a connecting block (8) on its side. The connecting block (8) is provided with a sliding adjustment rod (9) inside and a bracket (16) at one end of the adjustment rod (9). The bracket (16) is provided with a temperature sensor (10) inside and the temperature sensor (10) is electrically connected to the control panel (2) through a wire.

3. The magnetic stirring and oxygen content linkage control system for suspension cell culture according to claim 2, characterized in that: There is a certain amount of damping friction between the lifting block (7), adjusting rod (9) and temperature sensor (10) and the support rod (6), connecting block (8) and bracket (16).

4. The magnetic stirring and oxygen content linkage control system for suspension cell culture according to claim 1, characterized in that: The magnetic stir bar (27) is arranged in a cross shape and has slightly protruding sides on the surface.

5. The magnetic stirring and oxygen content linkage control system for suspension cell culture according to claim 2, characterized in that: The temperature sensor (10) is inserted into one of the vents (22) and extends into the interior of the culture vessel (11).

6. The magnetic stirring and oxygen content linkage control system for suspension cell culture according to claim 1, characterized in that: An electric heater (14) is provided on the side of the placement platform (4), and an electric heating wire (23) is provided on the side of the electric heater. The electric heating wire (23) extends into the interior of the placement platform (4) and is distributed in a serpentine structure.

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