Multi-channel protein purification instrument

Abstract

The utility model discloses a multichannel protein purification instrument, which belongs to the technical field of protein purification instruments and comprises a purification instrument body, a base is arranged at the bottom end of one side of the purification instrument body, moving devices are symmetrically arranged at the upper end of the base, driven frames are arranged at the upper ends of the moving devices, and positioning mounting components are arranged at the upper ends of the driven frames. An adjusting and placing assembly is arranged at the upper end of the positioning and mounting assembly; by arranging the adjusting and placing assembly, the opening plates with proper specifications can be selected according to use requirements to clamp a centrifugal tube, the applicability of the equipment is improved, the opening plates are convenient and stable to mount, the use effect of the equipment is ensured, the two groups of opening plates can synchronously move when the opening plates are clamped, and the opening plates can be limited after being clamped, so that the use efficiency of the equipment is improved. And the bottom plate can be conveniently and stably clamped and fixed by arranging the positioning and mounting assembly, so that the mounting convenience of the bottom plate is improved, and human resources are saved.

Description

Technical Field

[0001] This utility model belongs to the technical field of protein purification instruments, specifically relating to a multi-channel protein purification instrument. Background Technology

[0002] A protein purification instrument is a device used for protein purification. The protein purification system is designed for fully automated two-step affinity purification of proteins labeled with His and GST peptides. This user-friendly integrated system is superior to traditional gravity flow affinity chromatography techniques for the purification and desalting of affinity-labeled proteins.

[0003] Existing multichannel protein purification instruments are inconvenient to use when placing and clamping centrifuge tubes, and the stability of the centrifuge tubes cannot be guaranteed after clamping, which affects the protein purification effect. In addition, when installing the placement components, it is not possible to quickly position and install base plates of different sizes, which reduces work efficiency and the applicability of the equipment is poor. Utility Model Content

[0004] To address the problems mentioned in the background section, this invention provides a multi-channel protein purification instrument with strong applicability and stable centrifuge tube positioning and installation.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a multi-channel protein purification instrument, comprising a purification instrument body, a base being provided at the bottom of one side of the purification instrument body, a moving device being symmetrically provided at the upper end of the base, a driven frame being provided at the upper end of the moving device, a positioning and mounting component being provided at the upper end of the driven frame, and an adjustment and placement component being provided at the upper end of the positioning and mounting component.

[0006] Preferably, the adjustment and placement assembly includes a limiting groove, a limiting spring, a plug-in block, a limiting latch block, a fixed opening plate, a movable opening plate, a mounting frame, a connecting column, a limiting device, and a base plate. The upper end of the positioning and mounting assembly is provided with a base plate, the middle of the upper end of the base plate is provided with a limiting device, one side of the upper end of the base plate is provided with a connecting column, the upper end of the connecting column is provided with a mounting frame, the mounting frame has symmetrically formed limiting grooves inside, a fixed opening plate is provided inside the limiting groove, a movable opening plate is provided on one side of the fixed opening plate, a limiting latch block is provided at the upper end of the limiting groove, limiting springs are symmetrically arranged inside the limiting latch block, and one end of the limiting spring is provided with a plug-in block.

[0007] Preferably, the fixed opening plate and the movable opening plate are provided with limit blocks at both ends, the fixed opening plate has positioning holes on both sides inside, and the bottom end of the limit block is provided with a limit post corresponding to the positioning hole.

[0008] Preferably, the limiting device includes a pull rod, a guide plate, a positioning spring, a positioning tooth block, a guide post, a gear, a threaded rod, and a tapered post. The threaded rod is provided at the middle of the upper end of the base plate, and the upper end of the threaded rod is connected to the tapered post. Guide posts are symmetrically arranged on both sides of the tapered post. A gear is provided on the bottom surface of the threaded rod, and a guide plate is provided on one side of the gear. A pull rod is provided inside the guide plate, and a positioning spring is sleeved on the surface of the pull rod. A positioning tooth block is provided at one end of the pull rod corresponding to the gear.

[0009] Preferably, the positioning and installation assembly includes a positioning plate, a snap-fit ​​block, an intermediate stop block, a positioning post, a bidirectional lead screw, and a slider. The positioning plate is inserted into the upper end of the driven frame. An intermediate stop block is provided in the middle of the positioning plate. A bidirectional lead screw passes through the middle stop block. Slider blocks are symmetrically arranged on the surface of the bidirectional lead screw. Snap-fit ​​blocks are symmetrically arranged at the upper end of the sliders. Positioning posts are symmetrically arranged at the upper end of the intermediate stop block.

[0010] Preferably, the bottom end of the base plate has an installation hole corresponding to the positioning post, and the bottom end of the base plate has a guide groove corresponding to the snap-fit ​​block.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. This utility model, by setting an adjustable placement component, allows for the selection of appropriate specifications of open plates to clamp centrifuge tubes according to usage needs, improving the applicability of the equipment. The installation of the open plates is convenient and stable, ensuring the effectiveness of the equipment. When clamping the open plates, it can ensure that the two sets of open plates move synchronously, and after clamping, they can be limited to prevent loosening and ensure the strength of the equipment.

[0013] 2. By setting up positioning and installation components, this utility model can conveniently and stably clamp and fix the base plate, improve the convenience of base plate installation, save manpower, improve the applicability of equipment, and determine the installation position of the base plate through positioning columns, thereby improving the accuracy of installation. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This is a schematic diagram of the adjustment and placement component structure of this utility model;

[0016] Figure 3 This is a schematic diagram of the limiting device structure of this utility model;

[0017] Figure 4 This is a schematic diagram of the positioning and installation component structure of this utility model.

[0018] In the diagram: 1. Purifier body; 2. Base; 3. Moving device; 4. Driven frame; 5. Positioning and mounting assembly; 51. Positioning plate; 52. Snap-fit ​​block; 53. Intermediate stop block; 54. Positioning column; 55. Bidirectional lead screw; 56. Slider; 6. Adjustment and placement assembly; 60. Limiting groove; 61. Limiting spring; 62. Insertion block; 63. Limiting snap-fit ​​block; 64. Fixed opening plate; 65. Movable opening plate; 66. Mounting frame; 67. Connecting column; 68. Limiting device; 681. Pull rod; 682. Guide plate; 683. Positioning spring; 684. Positioning tooth block; 685. Guide column; 686. Gear; 687. Threaded rod; 688. Conical column; 69. Base plate. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Example 1

[0021] Please see Figure 1-4 The present invention provides the following technical solution: a multi-channel protein purification instrument, including a purification instrument body 1, a base 2 is provided at the bottom of one side of the purification instrument body 1, a moving device 3 is symmetrically provided at the upper end of the base 2, a driven frame 4 is provided at the upper end of the moving device 3, a positioning and mounting component 5 is provided at the upper end of the driven frame 4, and an adjustment and placement component 6 is provided at the upper end of the positioning and mounting component 5.

[0022] Specifically, the adjustment and placement assembly 6 includes a limiting groove 60, a limiting spring 61, a plug-in block 62, a limiting latch block 63, a fixed opening plate 64, a movable opening plate 65, a mounting bracket 66, a connecting column 67, a limiting device 68, and a base plate 69. The upper end of the positioning and installation assembly 5 is provided with a base plate 69, the middle of the upper end of the base plate 69 is provided with a limiting device 68, one side of the upper end of the base plate 69 is provided with a connecting column 67, the upper end of the connecting column 67 is provided with a mounting bracket 66, the mounting bracket 66 has symmetrically opened limiting grooves 60 inside, the limiting groove 60 has a fixed opening plate 64 inside, one side of the fixed opening plate 64 is provided with a movable opening plate 65, the upper end of the limiting groove 60 has a limiting latch block 63 inside, the limiting latch block 63 has symmetrically arranged limiting springs 61 inside, and one end of the limiting spring 61 is provided with a plug-in block 62.

[0023] By adopting the above technical solution, after the base plate 69 is installed, a suitable fixed opening plate 64 and a movable opening plate 65 are selected according to the specifications of the centrifuge tube for installation and use. The fixed opening plate 64 and the movable opening plate 65 are placed inside the limiting groove 60, and then the limiting spring 61 is pulled to drive the plug-in block 62 to retract, and the limiting block 63 is locked inside the upper end of the limiting groove 60. The limiting spring 61 is then released, and the plug-in block 62 is inserted into the mounting bracket 66, ensuring that the limiting block 63 can limit the fixed opening plate 64 and the movable opening plate 65.

[0024] Specifically, limit blocks are provided at both ends of the fixed opening plate 64 and the movable opening plate 65, positioning holes are provided on both sides of the interior of the fixed opening plate 64, and a limit post is provided at the bottom of the limit block 63 corresponding to the positioning hole.

[0025] By adopting the above technical solution, the limiting post at the bottom of the limiting block 63 is inserted into the positioning hole inside the fixed opening plate 64 to prevent the fixed opening plate 64 from moving and improve the stability of subsequent centrifuge tube clamping.

[0026] Specifically, the limiting device 68 includes a pull rod 681, a guide plate 682, a positioning spring 683, a positioning tooth block 684, a guide post 685, a gear 686, a threaded rod 687, and a conical post 688. The threaded rod 687 is provided in the middle of the upper end of the base plate 69. The upper end of the threaded rod 687 is connected to the conical post 688. Guide posts 685 are symmetrically arranged on both sides of the conical post 688. The gear 686 is provided on the bottom surface of the threaded rod 687. The guide plate 682 is provided on one side of the gear 686. The pull rod 681 is provided inside the guide plate 682. The positioning spring 683 is sleeved on the surface of the pull rod 681. The positioning tooth block 684 is provided at one end of the pull rod 681 corresponding to the gear 686.

[0027] By adopting the above technical solution, pulling the lever 681 causes the positioning tooth block 684 to separate from the gear 686. At this time, the positioning spring 683 is compressed by force. Then, the gear 686 is rotated to drive the threaded rod 687 to rotate. At this time, the conical column 688 moves upward along the gear 686. The conical column 688 abuts against the side of the two sets of movable opening plates 65 and pushes them to slide along the inside of the limiting groove 60, thereby clamping and fixing the centrifuge tube, ensuring the synchronicity of the movement of the two sets of movable opening plates 65. When the lever 681 is released, the positioning spring 683 resets, causing the positioning tooth block 684 to re-mesh with the gear 686, preventing the gear 686 from becoming loose, ensuring the clamping strength of the movable opening plate 65 on the centrifuge tube, and improving the stability of the equipment.

[0028] In this embodiment, after the base plate 69 is installed, select the appropriate fixed opening plate 64 and movable opening plate 65 according to the specifications of the centrifuge tube for installation. Place the fixed opening plate 64 and movable opening plate 65 inside the limiting groove 60, and then pull the limiting spring 61 to drive the plug block 62 to retract, locking the limiting block 63 inside the upper end of the limiting groove 60. Release the limiting spring 61, and at this time, the plug block 62 is inserted into the mounting bracket 66, ensuring that the limiting block 63 can limit the fixed opening plate 64 and movable opening plate 65. The limiting post at the bottom of the limiting block 63 is inserted into the positioning hole inside the fixed opening plate 64 to prevent the fixed opening plate 64 from moving and improve the stability of the centrifuge tube clamping.

[0029] After the installation of the fixed opening plate 64 and the movable opening plate 65 is completed, pulling the pull rod 681 causes the positioning tooth block 684 to separate from the gear 686. At this time, the positioning spring 683 is compressed by force. Then, the gear 686 is rotated to drive the threaded rod 687 to rotate. At this time, the conical column 688 moves upward along the gear 686. The conical column 688 abuts against the side of the two sets of movable opening plates 65 and pushes them to slide along the inside of the limiting groove 60, thereby clamping and fixing the centrifuge tube, ensuring the synchronicity of the movement of the two sets of movable opening plates 65. When the pull rod 681 is released, the positioning spring 683 resets, causing the positioning tooth block 684 to re-mesh with the gear 686, preventing the gear 686 from becoming loose, ensuring the clamping strength of the movable opening plate 65 on the centrifuge tube, and improving the stability of the equipment.

[0030] Example 2

[0031] The difference between this embodiment and embodiment 1 is that, specifically, the positioning and installation component 5 includes a positioning plate 51, a snap-fit ​​block 52, an intermediate stop block 53, a positioning post 54, a bidirectional lead screw 55, and a slider 56. The positioning plate 51 is inserted into the upper end of the driven frame 4. An intermediate stop block 53 is provided in the middle of the interior of the positioning plate 51. A bidirectional lead screw 55 passes through the interior of the intermediate stop block 53. Slider 56 is symmetrically arranged on the surface of the bidirectional lead screw 55. A snap-fit ​​block 52 is symmetrically arranged at the upper end of the slider 56. A positioning post 54 is symmetrically arranged at the upper end of the intermediate stop block 53.

[0032] By adopting the above technical solution, when using the purification instrument body 1, it is necessary to assemble each component first. Insert the positioning plate 51 into the upper end of the driven frame 4, and then insert the mounting hole at the bottom of the base plate 69 into the corresponding positioning column 54. At this time, rotate the bidirectional lead screw 55 to drive the slider 56 to slide along the inside of the positioning plate 51, so that the locking block 52 moves along the guide groove and abuts against the inner wall surface of the base plate 69, ensuring the accuracy and stability of the installation of the base plate 69.

[0033] Specifically, the bottom end of the base plate 69 has a mounting hole corresponding to the positioning post 54, and the bottom end of the base plate 69 has a guide groove corresponding to the snap-fit ​​block 52.

[0034] By adopting the above technical solution, the accuracy and stability of the base plate 69 installation are ensured, the equipment's performance is improved, and base plates 69 of different specifications can be clamped to limit their position, ensuring the applicability of the equipment.

[0035] In this embodiment, when using the purification instrument body 1, the components need to be assembled first. The positioning plate 51 is inserted into the upper end of the driven frame 4, and then the mounting hole at the bottom of the base plate 69 is inserted into the corresponding positioning post 54. At this time, rotating the bidirectional lead screw 55 drives the slider 56 to slide along the inside of the positioning plate 51, so that the locking block 52 moves along the guide groove and abuts against the inner wall surface of the base plate 69, ensuring the accuracy and stability of the base plate 69 installation, improving the use effect of the equipment, and can limit and clamp base plates 69 of different specifications to ensure the applicability of the equipment.

[0036] The structure and operating principle of the purification instrument body 1, the moving device 3, and the driven frame 4 in this utility model have been disclosed in a multi-channel protein purification instrument disclosed in Chinese patent application number 202420673608.X.

[0037] The working principle and usage process of this utility model: When using the purification instrument body 1, the components need to be assembled first. The positioning plate 51 is inserted into the upper end of the driven frame 4. Then, the mounting hole at the bottom of the base plate 69 is inserted into the corresponding positioning column 54. At this time, the bidirectional lead screw 55 is rotated to drive the slider 56 to slide along the inside of the positioning plate 51, so that the locking block 52 moves along the guide groove and abuts against the inner wall surface of the base plate 69, ensuring the accuracy and stability of the installation of the base plate 69, improving the use effect of the equipment, and can limit and clamp the base plate 69 of different specifications to ensure the applicability of the equipment.

[0038] After the base plate 69 is installed, select the appropriate fixed opening plate 64 and movable opening plate 65 according to the specifications of the centrifuge tubes for installation and use. Place the fixed opening plate 64 and movable opening plate 65 inside the limiting groove 60, and then pull the limiting spring 61 to drive the plug block 62 to retract, locking the limiting block 63 inside the upper end of the limiting groove 60. Release the limiting spring 61, and at this time, the plug block 62 is inserted into the mounting bracket 66 to ensure that the limiting block 63 can limit the fixed opening plate 64 and movable opening plate 65. The limiting post at the bottom of the limiting block 63 is inserted into the positioning hole inside the fixed opening plate 64 to prevent the fixed opening plate 64 from moving and improve the stability of the centrifuge tube clamping in the future.

[0039] After the installation of the fixed opening plate 64 and the movable opening plate 65 is completed, pulling the pull rod 681 causes the positioning tooth block 684 to separate from the gear 686. At this time, the positioning spring 683 is compressed by force. Then, the gear 686 is rotated to drive the threaded rod 687 to rotate. At this time, the conical column 688 moves upward along the gear 686. The conical column 688 abuts against the side of the two sets of movable opening plates 65 and pushes them to slide along the inside of the limiting groove 60, thereby clamping and fixing the centrifuge tube, ensuring the synchronicity of the movement of the two sets of movable opening plates 65. When the pull rod 681 is released, the positioning spring 683 resets, causing the positioning tooth block 684 to re-mesh with the gear 686, preventing the gear 686 from becoming loose, ensuring the clamping strength of the movable opening plate 65 on the centrifuge tube, and improving the stability of the equipment.

[0040] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multi-channel protein purification instrument, comprising a purification instrument body (1), wherein a base (2) is provided at the bottom of one side of the purification instrument body (1), and a moving device (3) is symmetrically provided at the upper end of the base (2), and a driven frame (4) is provided at the upper end of the moving device (3), characterized in that: The upper end of the driven frame (4) is provided with a positioning and mounting component (5), and the upper end of the positioning and mounting component (5) is provided with an adjustment and placement component (6). The adjustment and placement assembly (6) includes a limiting groove (60), a limiting spring (61), a plug-in block (62), a limiting latch (63), a fixed opening plate (64), a movable opening plate (65), a mounting bracket (66), a connecting column (67), a limiting device (68), and a base plate (69). The upper end of the positioning and installation assembly (5) is provided with a base plate (69), the middle of the upper end of the base plate (69) is provided with a limiting device (68), and one side of the upper end of the base plate (69) is provided with a connecting column (68). 7) A mounting bracket (66) is provided at the upper end of the connecting column (67). A limiting groove (60) is symmetrically opened inside the mounting bracket (66). A fixed opening plate (64) is provided inside the limiting groove (60). A movable opening plate (65) is provided on one side of the fixed opening plate (64). A limiting block (63) is provided at the upper end of the limiting groove (60). A limiting spring (61) is symmetrically provided inside the limiting block (63). A plug-in block (62) is provided at one end of the limiting spring (61).

2. The multichannel protein purification instrument according to claim 1, characterized in that: Limiting blocks are provided at both ends of the fixed opening plate (64) and the movable opening plate (65). Positioning holes are provided on both sides of the interior of the fixed opening plate (64), and a limiting post is provided at the bottom of the limiting block (63) corresponding to the positioning hole.

3. The multichannel protein purification instrument according to claim 1, characterized in that: The limiting device (68) includes a pull rod (681), a guide plate (682), a positioning spring (683), a positioning tooth block (684), a guide post (685), a gear (686), a threaded rod (687), and a conical post (688). The threaded rod (687) is provided in the middle of the upper end of the base plate (69). The upper end of the threaded rod (687) is connected to the conical post (688). Guide posts (685) are symmetrically arranged on both sides of the conical post (688). The gear (686) is provided on the bottom surface of the threaded rod (687). The guide plate (682) is provided on one side of the gear (686). The pull rod (681) is provided inside the guide plate (682). The positioning spring (683) is sleeved on the surface of the pull rod (681). The positioning tooth block (684) is provided at one end of the pull rod (681) corresponding to the gear (686).

4. The multichannel protein purification instrument according to claim 1, characterized in that: The positioning and installation assembly (5) includes a positioning plate (51), a snap-fit ​​block (52), an intermediate stop block (53), a positioning post (54), a two-way lead screw (55), and a slider (56). The positioning plate (51) is inserted into the upper end of the driven frame (4). An intermediate stop block (53) is provided in the middle of the interior of the positioning plate (51). A two-way lead screw (55) passes through the interior of the intermediate stop block (53). A slider (56) is symmetrically arranged on the surface of the two-way lead screw (55). A snap-fit ​​block (52) is symmetrically arranged at the upper end of the slider (56). A positioning post (54) is symmetrically arranged at the upper end of the intermediate stop block (53).

5. A multichannel protein purification instrument according to claim 1, characterized in that: The bottom end of the base plate (69) is provided with a mounting hole corresponding to the positioning post (54), and the bottom end of the base plate (69) is provided with a guide groove corresponding to the snap-fit ​​block (52).

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