Fermentation tank for preparing phenylalanine

Abstract

According to the fermentation tank for preparing phenylalanine provided by the utility model, the constant-temperature heating pipe is arranged on the inner wall of the inner layer of the tank body, so that the temperature in the tank body is actively regulated and controlled, the temperature in the tank body is compensated, and fermentation can be efficiently carried out at a constant temperature; meanwhile, heat exchange inside and outside the tank body is remarkably reduced by arranging the insulating layer, heat loss of a high-temperature medium or cold loss of a low-temperature medium is reduced, temperature stability in the tank body is further maintained, and fermentation efficiency is improved. Secondly, a spiral aeration pipe is arranged, oxygen entering from an air inlet pipe is released into the tank body in the form of tiny bubbles through aeration holes, and four first rotating shafts are arranged around the air inlet pipe to drive a negative pressure area generated by rotation of first stirring blades to accelerate cutting of oxygen bubbles generated by central aeration, so that the contact area of gas and liquid is effectively increased; the oxygen dissolving efficiency and the oxygen dissolving uniformity are improved while the phenylalanine fermentation materials are mixed more uniformly, so that the fermentation effect is improved.

Description

Technical Field

[0001] This utility model relates to the field of enzyme preparation processing technology, and in particular to a fermenter for preparing phenylalanine. Background Technology

[0002] Phenylalanine is one of the essential amino acids for the human body. In the body, most of it is oxidized to tyrosine by phenylalanine hydroxylase, and together with tyrosine, it synthesizes important neurotransmitters and hormones, participating in the body's sugar and fat metabolism.

[0003] There are three main methods for preparing phenylalanine: microbial fermentation, protein hydrolysis, and organic synthesis. In the microbial fermentation process, the metabolic activity of the microorganisms is closely related to gas solubility and fermentation temperature. When the dissolved oxygen level in the fermentation broth is too low, such as below 15%, the growth of microorganisms such as *Corynebacterium glutamicum* is inhibited, leading to the accumulation of metabolic byproducts and thus reducing the yield of phenylalanine. Conversely, controlling the dissolved oxygen within a suitable range, such as 30%-40%, can significantly improve the metabolic activity of the microorganisms and promote the synthesis of phenylalanine. Furthermore, experiments have shown that *Corynebacterium glutamicum* achieves the highest phenylalanine yield at 30-33℃, and temperature fluctuations exceeding ±1℃ significantly affect cell metabolism and product synthesis.

[0004] Therefore, in order to improve the efficiency of phenylalanine preparation by microbial fermentation, it is necessary to provide a fermenter that is at a constant temperature and can provide an appropriate amount of dissolved oxygen. Utility Model Content

[0005] In view of the technical problems of low fermentation efficiency caused by low and uneven dissolved oxygen content and unstable temperature in the fermenter in the existing technology, this utility model provides a fermenter for preparing phenylalanine.

[0006] A fermenter for preparing phenylalanine includes a tank body and a lid for sealing the tank opening. The tank body has a discharge pipe at its bottom and an exhaust pipe on one side of its top. A pressure relief valve is installed on the exhaust pipe. The tank body includes an inner tank layer and an outer tank layer fitted over the inner tank layer. A heat insulation layer is provided between the inner and outer tank layers. The inner wall of the inner tank layer is provided with several constant-temperature heating pipes. An air inlet pipe is penetrating the center of the lid. Four first rotating shafts and a feed pipe are also penetrating the lid, with each first rotating shaft surrounding the air inlet pipe. One end of the first rotating shaft is connected to a first driving mechanism, and the other end extends into the interior of the tank. Several first stirring blades are respectively provided at both ends of the first rotating shaft inside the tank. A spiral aeration pipe is provided inside the tank, with aeration holes evenly spaced on the spiral aeration pipe, and the spiral aeration pipe is connected to the air inlet pipe. A hydrophobic and antibacterial filter and a gas flow control valve are provided on the air inlet pipe, and the air inlet pipe is connected to an oxygen supply mechanism. A dissolved oxygen sensor is provided on the inner wall of the inner layer of the tank. A pressure detection mechanism for detecting the internal air pressure of the tank is also provided on the top of the tank lid.

[0007] Preferably, the bottom of the tank is provided with a plurality of second rotating shafts, the second rotating shafts being located below the first rotating shaft and being perpendicular to the first rotating shaft; the second rotating shafts are provided with a plurality of second stirring blades, and one end of the second rotating shaft passes through the tank and is connected to a second driving mechanism.

[0008] Preferably, both the first drive mechanism and the second drive mechanism are variable frequency motors.

[0009] Preferably, the inner layer of the tank is recessed along the direction close to the outer layer of the tank to form a plurality of grooves, and the constant temperature heating tube is disposed in the grooves.

[0010] Preferably, the air pressure detection mechanism includes an air pressure sensor and an air pressure gauge disposed on the can lid.

[0011] Preferably, a filter screen is provided at the connection between the discharge pipe and the tank.

[0012] Preferably, the insulation layer is a rock wool layer or a polyurethane foam material layer.

[0013] Preferably, a check valve is also provided on the air inlet pipe, and a solenoid valve is provided on both the feed pipe and the discharge pipe.

[0014] Preferably, the top of the can lid is provided with a pair of C-shaped handles; the bottom of the can body is provided with several support feet.

[0015] Preferably, the oxygen supply mechanism is an oxygen supply pump, which is connected to the air inlet pipe via an air delivery pipe.

[0016] The beneficial effects of this utility model are as follows: This utility model provides a fermenter for preparing phenylalanine. By setting a constant temperature heating tube on the inner wall of the inner layer of the tank, the internal temperature of the tank is actively regulated and compensated for, which can ensure that the fermentation can be carried out efficiently at a constant temperature. At the same time, the heat insulation layer significantly reduces the heat exchange between the inside and outside of the tank, reduces the heat loss of high temperature medium or the loss of cold energy of low temperature medium, further maintains the temperature stability inside the tank, and improves the fermentation efficiency.

[0017] Secondly, a spiral aeration pipe is installed, which releases oxygen from the air inlet pipe into the tank as tiny bubbles through the aeration holes. Four first rotating shafts are set around the air inlet pipe, which drive the first stirring blades to rotate, creating a negative pressure zone that accelerates the cutting of oxygen bubbles generated by the central aeration. This effectively increases the contact area between gas and liquid, making the phenylalanine fermentation material more uniformly mixed while improving dissolved oxygen efficiency and uniformity, thereby improving the fermentation effect.

[0018] Finally, by setting up a dissolved oxygen sensor to monitor the dissolved oxygen level in the tank in real time, and in conjunction with a gas flow control valve, the oxygen supply can be dynamically adjusted, thereby avoiding situations where too much or too little dissolved oxygen affects the normal fermentation process, making the fermentation more efficient. Attached Figure Description

[0019] Figure 1 A three-dimensional structural diagram of a fermenter for preparing phenylalanine provided by this utility model;

[0020] Figure 2 This is a cross-sectional structural diagram of a fermenter for preparing phenylalanine, provided by this utility model.

[0021] Attached Figure Labels

[0022] 1. Tank body; 101. Inner layer of tank; 102. Outer layer of tank; 2. Tank cover; 3. Discharge pipe; 4. Exhaust pipe; 5. Pressure relief valve; 6. Pressure sensor; 7. Pressure gauge; 8. Insulation layer; 9. Constant temperature heating tube; 10. Air inlet pipe; 11. First rotating shaft; 12. Feed pipe; 13. First drive mechanism; 14. First stirring blade; 15. Spiral aeration pipe; 16. Aeration hole; 17. Dissolved oxygen sensor; 18. Hydrophobic and sterilizing filter; 19. Gas flow control valve; 20. Second rotating shaft; 21. Second stirring blade; 22. Filter screen; 23. Check valve; 24. Solenoid valve; 25. C-shaped handle; 26. Support foot. Detailed Implementation

[0023] The embodiments described below are merely some embodiments of this utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without inventive effort are within the scope of protection of this utility model.

[0024] Reference Figure 1 As shown, this utility model discloses a fermenter for preparing phenylalanine, including a tank body 1 and a tank lid 2 for sealing the opening of the tank body.

[0025] Specifically, the tank body 1 is provided with a discharge pipe 3 at the bottom and an exhaust pipe 4 on one side of the top of the tank body 1; a pressure relief valve 5 is provided on the exhaust pipe 4. The top of the tank cover 1 is also provided with a pressure detection mechanism (not shown in the figure) for detecting the internal air pressure of the tank.

[0026] The exhaust pipe 4 and the pressure relief valve 5 form a mechanical safety valve structure. When the gas pressure in tank 1 exceeds the set threshold, the pressure relief valve 5 automatically opens to release gas, ensuring that the gas pressure inside tank 1 remains within the normal threshold range, thus guaranteeing the safe and efficient fermentation process in tank 1.

[0027] The pressure detection mechanism includes a pressure sensor 6 and a pressure gauge 7 mounted on the tank cover 2. The pressure sensor 6 detects and feeds back the pressure data inside the tank 1 in real time, which is then displayed on the pressure gauge 7. This mechanism, in conjunction with the exhaust pipe 4 and the pressure relief valve 5, allows for precise control of pressure parameters during fermentation, maintaining the optimal pressure environment required for fermentation, thereby effectively improving microbial metabolic efficiency and accelerating the fermentation rate.

[0028] The tank body 1 includes an inner tank layer 101 and an outer tank layer 102 sleeved around the inner tank layer; a thermal insulation layer 8 is provided between the inner tank layer 101 and the outer tank layer 102; the inner wall of the inner tank layer 101 is provided with a plurality of constant temperature heating tubes 9. In this embodiment, the thermal insulation layer 8 is a rock wool layer or a polyurethane foam material layer. The constant temperature heating tubes 9 can convert electrical energy into heat energy and have a built-in temperature controller, which can maintain the set temperature by automatically adjusting the current to achieve precise temperature control. The rock wool layer or the polyurethane foam material layer has a low thermal conductivity, which can effectively prevent heat convection and conduction, and achieve a good thermal insulation effect.

[0029] The insulation layer 8 reduces heat exchange between the inside and outside of tank 1, minimizing heat loss from high-temperature media or cold loss from low-temperature media, thus maintaining a stable temperature inside tank 1 and ensuring efficient fermentation at a suitable temperature. Simultaneously, a constant-temperature heating pipe 9 is installed on the inner wall of the inner layer 101 of the tank to actively regulate and compensate for the internal temperature of tank 1, ensuring fermentation proceeds at a suitable temperature. This allows for precise temperature control of the materials inside tank 1, maintaining a constant temperature during fermentation and promoting efficient phenylalanine fermentation production. Furthermore, the constant-temperature heating pipe 9 can heat, dry, and sterilize the inside of tank 1 before production, creating a dry and sterile reaction environment to ensure stable fermentation without interference.

[0030] In this embodiment, the inner layer 101 of the tank is recessed along the direction close to the outer layer 102 of the tank to form several grooves (not shown in the figure), and the constant temperature heating tube 9 is disposed in the grooves. The grooves reduce the space occupied by the constant temperature heating tube 9 in the inner layer 101 of the tank while maintaining good heat transfer efficiency.

[0031] An air inlet pipe 10 is provided through the center of the tank cover 2; four first rotating shafts 11 and a feed pipe 12 are also provided through the tank cover 2, and the first rotating shafts 11 are arranged around the air inlet pipe 12; one end of each first rotating shaft 11 is connected to a first driving mechanism 13, and the other end extends into the interior of the tank body 1, and several first stirring blades 14 are respectively provided at both ends of the first rotating shafts 11 inside the tank body 1; a spiral aeration pipe 15 is provided inside the tank body 1, and aeration holes 16 are opened at equal intervals on the spiral aeration pipe 15, and the spiral aeration pipe 15 is connected to the air inlet pipe 12. A dissolved oxygen sensor 17 is provided on the inner wall of the inner layer 101 of the tank. A hydrophobic and sterilizing filter 18 and a gas flow control valve 19 are provided on the air inlet pipe 12, and the air inlet pipe 12 is connected to an oxygen supply mechanism (not shown in the figure). In this embodiment, the oxygen supply mechanism is an oxygen supply pump, and the oxygen supply pump is connected to the air inlet pipe 12 through a gas delivery pipe (not shown in the figure).

[0032] Oxygen entering the air inlet pipe 10 passes through the spiral aeration pipe 15 and is released into the phenylalanine fermentation broth inside the tank 1 in the form of microbubbles through the aeration holes 16 of the spiral aeration pipe 15. The three-dimensional structure of the spiral aeration pipe 15 allows oxygen to diffuse better into the fermentation broth and effectively increases the contact area between gas and liquid, thereby improving the rate and uniformity of dissolved oxygen in the fermentation broth. This ensures a sufficient oxygen supply to all areas within the tank 1, guaranteeing fermentation efficiency. Simultaneously, four first rotating shafts 11 are arranged around the air inlet pipe 12 for multi-axis stirring. The negative pressure zone generated by the rotation of the first stirring blades 14 accelerates the cutting of oxygen bubbles generated by the central aeration, effectively enhancing the stirring and dispersion effect of oxygen. This makes the phenylalanine fermentation material more uniformly mixed while improving dissolved oxygen efficiency and uniformity, thereby improving the fermentation effect. Finally, by setting a dissolved oxygen sensor 17 to monitor the dissolved oxygen level in the tank 1 in real time, and in conjunction with the gas flow control valve 19, the oxygen supply can be dynamically adjusted, thereby avoiding situations where too much or too little dissolved oxygen affects the normal fermentation process, making the fermentation more efficient. The hydrophobic and sterile filter 18 can filter out any microorganisms that may be present in the oxygen entering through the air inlet pipe 10, ensuring that the oxygen is sterile and preventing contamination of the material in the tank 1 by miscellaneous bacteria.

[0033] The bottom of the tank body 1 is also provided with several second rotating shafts 20. The second rotating shafts 20 are located below the first rotating shaft 11 and are arranged perpendicular to the first rotating shaft 11. Several second stirring blades 21 are provided on the second rotating shafts 20, and one end of the second rotating shaft 20 passes through the tank body 1 and is connected to a second driving mechanism (not shown in the figure).

[0034] By providing several second rotating shafts 20 at the bottom of the tank 1, and several second stirring blades 21 on the second rotating shafts 20, the material at the bottom of the tank 1 can be stirred, preventing the phenylalanine fermentation material from settling at the bottom of the tank 1 while the stirring force of the first rotating shaft 11 is insufficient, resulting in uneven mixing of the reaction material. The cooperation of the first rotating shaft 11 and the second rotating shaft 12 can greatly accelerate the stirring efficiency of the reaction material and improve the stirring effect.

[0035] The first drive mechanism 13 and the second drive mechanism are both variable frequency motors. By adjusting the variable frequency motors, the first rotating shaft 11 and the second rotating shaft 12 can be made to stir the phenylalanine fermentation material at different rotation speeds, thereby achieving different stirring effects.

[0036] In some embodiments, a filter screen 22 is provided at the connection between the discharge pipe 3 and the tank 1. The filter screen 22 can intercept incompletely reacted phenylalanine fermentation material, preventing the phenylalanine fermentation material from clogging the discharge pipe 3.

[0037] In some embodiments, a check valve 23 is also provided on the air inlet pipe to prevent the medium in the tank 1 from flowing back into the air inlet pipe 10 and affecting normal air intake. A solenoid valve 24 is provided on both the feed pipe 10 and the discharge pipe 3 to control the entry and exit of phenylalanine fermentation material.

[0038] In some embodiments, the top of the can lid 2 is provided with a pair of C-shaped handles 25; the bottom of the can body 1 is provided with a plurality of support feet 26.

[0039] This invention provides a fermenter for preparing phenylalanine. By installing a constant-temperature heating pipe 9 and an insulation layer 8 on the inner wall of the inner layer of the tank, the temperature inside the tank 1 is kept constant, thereby effectively improving the fermentation efficiency. Simultaneously, a spiral aeration pipe 15 and four first rotating shafts 11 drive first stirring blades 14 to stir the phenylalanine fermentation material, making the phenylalanine fermentation material more uniformly mixed while improving dissolved oxygen efficiency and uniformity, thus enhancing the fermentation effect.

[0040] The above-disclosed embodiments are merely some preferred embodiments of the present utility model, and should not be construed as limiting the scope of the present utility model. Therefore, any equivalent changes made in accordance with the scope of the present utility model patent application shall still fall within the scope of the present utility model.

Claims

1. A fermenter for preparing phenylalanine, comprising a tank body and a lid for sealing the opening of the tank body, characterized in that, The tank body is provided with a discharge pipe at the bottom and an exhaust pipe on one side of the top of the tank body; a pressure relief valve is provided on the exhaust pipe; the tank body includes an inner tank layer and an outer tank layer sleeved outside the inner tank layer; an insulation layer is provided between the inner tank layer and the outer tank layer; The inner wall of the inner layer of the tank is provided with several constant temperature heating tubes; an air inlet pipe is provided through the center of the tank cover; four first rotating shafts and a feed pipe are also provided through the tank cover, and the first rotating shafts are arranged around the air inlet pipe; one end of the first rotating shaft is connected to a first driving mechanism, and the other end extends into the inside of the tank, and several first stirring blades are provided at both ends of the first rotating shaft inside the tank. The tank is equipped with a spiral aeration pipe inside, and aeration holes are opened at equal intervals on the spiral aeration pipe. The spiral aeration pipe is connected to the air inlet pipe. The air inlet pipe is equipped with a hydrophobic and sterilizing filter and a gas flow control valve. The air inlet pipe is connected to the oxygen supply mechanism. An oxygen sensor is installed on the inner wall of the inner layer of the tank; a pressure detection mechanism for detecting the internal air pressure of the tank is also provided on the top of the tank cover.

2. The fermenter for preparing phenylalanine according to claim 1, characterized in that, The bottom of the tank is also provided with several second rotating shafts, which are located below the first rotating shaft and are perpendicular to the first rotating shaft; several second stirring blades are provided on the second rotating shafts, and one end of the second rotating shaft passes through the tank and is connected to a second driving mechanism.

3. A fermenter for preparing phenylalanine according to claim 1, characterized in that, Both the first drive mechanism and the second drive mechanism are variable frequency motors.

4. A fermenter for preparing phenylalanine according to claim 1, characterized in that, The inner layer of the tank is recessed along the direction close to the outer layer of the tank to form several grooves, and the constant temperature heating tube is disposed in the grooves.

5. A fermenter for preparing phenylalanine according to claim 1, characterized in that, The air pressure detection mechanism includes an air pressure sensor and an air pressure gauge installed on the can lid.

6. A fermenter for preparing phenylalanine according to claim 1, characterized in that, A filter screen is installed at the connection between the discharge pipe and the tank.

7. A fermenter for preparing phenylalanine according to claim 1, characterized in that, The insulation layer is a rock wool layer or a polyurethane foam material layer.

8. A fermenter for preparing phenylalanine according to claim 1, characterized in that, The air inlet pipe is also equipped with a check valve, and both the feed pipe and the discharge pipe are equipped with a solenoid valve.

9. A fermenter for preparing phenylalanine according to claim 1, characterized in that, The top of the can lid is provided with a pair of C-shaped handles; the bottom of the can body is provided with several support feet.

10. A fermenter for preparing phenylalanine according to claim 1, characterized in that, The oxygen supply mechanism is an oxygen pump, which is connected to the air inlet pipe via an air delivery pipe.

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